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Intermediate monocyte

Enrico Ammirati, Francesco Moroni, Marco Magnoni, Simona Di Terlizzi, Chiara Villa, Federico Sizzano, Alessio Palini, Katia Garlaschelli, Fernanda Tripiciano, Isabella Scotti, Alberico Luigi Catapano, Angelo A Manfredi, Giuseppe Danilo Norata, Paolo G Camici
BACKGROUND AND AIMS: Monocytes are known to play a key role in the initiation and progression of atherosclerosis and contribute to plaque destabilization through the generation of signals that promote inflammation and neoangiogenesis. In humans, studies investigating the features of circulating monocytes in advanced atherosclerotic lesions are lacking. METHODS: Patients (mean age 69 years, 56% males) with intermediate asymptomatic carotid stenosis (40-70% in diameter) were evaluated for maximal stenosis in common carotid artery, carotid bulb and internal carotid artery, overall disease burden as estimated with total plaque area (TPA), greyscale and neovascularization in 244 advanced carotid plaques...
October 6, 2016: Atherosclerosis
M B Huante, S Gupta, V C Calderon, S J Koo, M Sinha, B A Luxon, N J Garg, J J Endsley
Pathogens frequently exploit or evade inflammasome activation in order to survive and proliferate. Alternatively, inadequate inflammasome activation by attenuated microorganisms or adjuvanted subunit vaccines may contribute to poor longevity of protection. To further understand these pathways, we determined the differential inflammasome transcriptome of human THP monocyte-derived macrophages in response to Mycobacterium bovis BCG, as compared to LPS or Trypanosoma cruzi. The results identify the highly specific innate recognition programs associated with inflammasome activation by human macrophages exposed to these microbial stimuli...
September 28, 2016: Tuberculosis
Priyanka Gaur, Arpita Myles, Ramnath Misra, Amita Aggarwal
Microarray of peripheral blood (PB) and synovial fluid mononuclear cells (PBMC, SFMC) of patients with Enthesitis-related-arthritis (JIA-ERA) has shown the involvement of monocytes. On basis of CD14 and CD16 expression monocytes are classified as classical, intermediate and non-classical. In response to Toll-like Receptor (TLR) stimulation intermediate-monocytes produce pro-inflammatory cytokines and play a role in inflammatory diseases. Thus we have studied the microarray profile of monocytes, frequency of their subsets and cytokine production...
October 5, 2016: Clinical and Experimental Immunology
Aurélie Gabrion, Isabelle Hmitou, Despina Moshous, Bénédicte Neven, Alain Lefèvre-Utile, Jean-Sébastien Diana, Félipe Suarez, Capucine Picard, Stéphane Blanche, Alain Fischer, Marina Cavazzana, Fabien Touzot
BACKGROUND: Chronic granulomatous disease (CGD) is primary immunodeficiency caused by defective production of reactive oxygen species in phagocytic cells that results in life-threatening infections and severe inflammatory manifestations. The treatment of inflammatory manifestations remains challenging, as it can be associated with an increased risk of infections. Previous studies have shown that CGD phagocytes display a defect in autophagy and a reactive oxygen species-independent activation of the inflammasome...
October 1, 2016: Journal of Allergy and Clinical Immunology
Brett A Sponseller, Sandra K Clark, Jessica Gilbertie, David M Wong, Kate Hepworth, Sarah Wiechert, Prashanth Chandramani, Beatrice T Sponseller, Cody J Alcott, Bryan Bellaire, Andrew C Petersen, Douglas E Jones
Reactive intermediates contribute to innate immunity by providing phagocytes with a mechanism of defense against bacteria, viruses and parasites. To better characterize the role of CD154 in the production of reactive intermediates, we cloned and expressed recombinant equine CD154 (reqCD154) in Chinese Hamster Ovary (CHO). In co-culture experiments, CHO cells ectopically expressing reqCD154 elicited superoxide production in monocyte-derived macrophages (MDM). Collectively, our results indicate that regulation of CD154 expression plays a role in innate host defenses...
November 1, 2016: Veterinary Immunology and Immunopathology
Maria E Stålhammar, Lena Douhan Håkansson, Anders Jonzon, Richard Sindelar
BACKGROUND: A prerequisite for an effective innate immunity is the migrative ability of neutrophils to respond to inflammatory and infectious agents such as the intermediate interleukin (IL)-8 and the end-target formyl-methionyl-leucyl-phenylalanine (fMLP) chemoattractants. The aim was to study the chemotactic capacity of neutrophils from newborn infants and adults in response to IL-8 and the bacterial peptide fMLP. METHODS: In the under-agarose cell migration assay, isolated leukocytes from healthy adults and from cord blood of healthy term newborn infants were studied with dose responses towards IL-8 and fMLP...
October 3, 2016: Upsala Journal of Medical Sciences
Elise L Donovan, Emma J Buckels, Serina Hancock, Danielle Smeitink, Mark H Oliver, Frank H Bloomfield, Anne L Jaquiery
Twins are often born small and early and have increased risk of obesity and diabetes later in life. Twin conception in sheep, regardless of whether the pregnancy continues as twins or is reduced to singleton in early gestation, alters offspring growth trajectory and body composition in young adulthood. We hypothesized that twin conception would result in insulin resistance in adulthood, with insulin-resistant adipose tissue and skeletal muscle phenotypes. At 3 years of age, body weight was not different among singletons, twins, and reductions; females weighed less than males...
September 27, 2016: Reproductive Sciences
Sam Famenini, Elizabeth A Rigali, Henry M Olivera-Perez, Johnny Dang, Michael To Chang, Ramesh Halder, Rammohan V Rao, Matteo Pellegrini, Verna Porter, Dale Bredesen, Milan Fiala
Monocyte/macrophages of patients with mild cognitive impairment (MCI) and Alzheimer disease (AD) are defective in phagocytosis and degradation amyloid-β 1-42 (Aβ1-42), but are improved by ω-3 fatty acids (ω-3). The hypothesis of this study was that active Aβ1-42 phagocytosis by macrophages prevents brain amyloidosis and thus maintains cognition. We studied the effects of self-supplementation with a drink with ω-3, antioxidants, and resveratrol on Mini-Mental State Examination (MMSE) scores, macrophage M1M2 phenotype (the ratio of inflammatory CD54+CD80 and proresolution markers CD163+CD206), and Aβ1-42 phagocytosis in patients initially diagnosed as having MCI or subjective cognitive impairment (SCI)...
September 27, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Zhichen Xing, Junyan Han, Xing Hao, Jinhong Wang, Chunjing Jiang, Yu Hao, Hong Wang, Xueying Wu, Liwei Shen, Xiaojun Dong, Tong Li, Guoli Li, Jianping Zhang, Xiaotong Hou, Hui Zeng
BACKGROUND: As immune regulatory and effector cells, monocytes play an important role in the blood-extracorporeal circuit contact-related acute lung injury in patients undergoing cardiopulmonary bypass (CPB). However, circulating monocytes are phenotypically and functionally heterogeneous, so we characterised how immature monocytes affect acute lung injury induced by CPB. METHODS: The identification and dynamic changes in monocyte subsets were monitored by flow cytometry in patients undergoing CPB and in a rat model of CPB...
September 22, 2016: Thorax
Alexandru Schiopu, Eva Bengtsson, Isabel Gonçalves, Jan Nilsson, Gunilla Nordin Fredrikson, Harry Björkbacka
BACKGROUND: Myeloid cells play a central role in atherosclerosis. We investigated the associations between the plasma levels of growth factors and chemokines that regulate myeloid cell homeostasis and function and the risk of first-time acute coronary events in middle-aged persons. METHODS AND RESULTS: We measured baseline plasma levels of macrophage colony-stimulating factor; monocyte chemotactic protein 1; C-C motif chemokine ligands 3, 4, and 20; C-X-C motif chemokine ligands 1, 6, and 16; and C-X3-C motif chemokine ligand 1 in 292 participants who had a coronary event during follow-up and 366 controls matched for age, sex, and time of inclusion who remained event free...
September 2016: Journal of the American Heart Association
Hege Lund, Preben Boysen, Caroline Piercey Åkesson, Anna Monika Lewandowska-Sabat, Anne K Storset
The dynamics of skin-draining cells following infection or vaccination provide important insight into the initiation of immune responses. In this study, the local recruitment and activation of immune cells in draining lymph nodes (LNs) was studied in calves in an adjuvant-induced inflammation. A transient but remarkably strong recruitment of monocytes was demonstrated after onset of inflammation, constituting up to 41% of live cells in the draining LNs after 24 h. Numerous CD14(+) cells were visualized in subcutaneous tissues and draining LNs, and the majority of these cells did not express dendritic cell-associated markers CD205 and CD11c...
2016: Frontiers in Immunology
Moritz Wildgruber, Maria Czubba, Teresa Aschenbrenner, Heiko Wendorff, Alexander Hapfelmeier, Almut Glinzer, Matthias Schiemann, Alexander Zimmermann, Hans-Henning Eckstein, Hermann Berger, Walter A Wohlgemuth, Reinhard Meier, Peter Libby, Alma Zernecke
BACKGROUND AND AIMS: We aimed at studying the association of three major human monocyte subsets after percutaneous transluminal angioplasty (PTA) in patients with femoropopliteal disease. METHODS: We prospectively studied 67 sequential patients (40 male, 27 female; mean age 71 ± 11 years) treated with femoropopliteal angioplasty. Multi-color flow cytometry characterized monocyte subsets from venous blood for expression of CD14 and CD16 and intracellular myeloperoxidase (MPO) prior to, and 3, 6 and 12 months post PTA...
October 2016: Atherosclerosis
Konstantin A Krychtiuk, Max Lenz, Lorenz Koller, Maria C Honeder, Lisa Wutzlhofer, Chao Zhang, Lijian Chi, Gerald Maurer, Alexander Niessner, Kurt Huber, Johann Wojta, Gottfried Heinz, Walter S Speidl
Although patients admitted to an intensive care unit (ICU) suffer from various pathologies, many develop a systemic inflammatory response syndrome (SIRS). As key regulators of innate immunity, monocytes may be crucially involved in SIRS development. Monocytes can be distinguished into three subsets: Classical monocytes (CD14++CD16-; CM), non-classical monocytes (CD14+CD16++CCR2-; NCM) and intermediate monocytes (CD14++CD16+CCR2+; IM). The aim of this prospective, observational study was to analyse whether monocyte subset distribution is associated with 30-day survival in critically ill patients...
September 8, 2016: Thrombosis and Haemostasis
Brianna Pomeroy, Anja Sipka, Suzanne Klaessig, Ynte Schukken
Changes in monocyte and dendritic cell populations during bovine pregnancy and lactation remain poorly described despite the key roles these cells play in immune tolerance and activation. Using a prospective longitudinal study, we characterized CD14+ monocyte-derived dendritic cell (moDC) differentiation and maturation and captured monocyte composition dynamics from mid-gestation through calving and into the subsequent lactation in dairy cows (n=7). First, we measured absolute counts of classical (CD14+CD16-, cM), intermediate (CD14+CD16+, intM), and nonclassical (CD14-CD16+, ncM) monocytes in the blood and determined proportions of individual subsets within the total monocyte population...
August 30, 2016: Journal of Reproductive Immunology
Clovis S Palmer, Joshua J Anzinger, Tiffany R Butterfield, Joseph M McCune, Suzanne M Crowe
Monocytes are innate immune cells that can be activated by pathogens and inflammation associated with certain chronic inflammatory diseases. Activation of monocytes induces effector functions and a concomitant shift from oxidative to glycolytic metabolism that is accompanied by increased glucose transporter expression. This increased glycolytic metabolism is also observed for trained immunity of monocytes, a form of innate immunological memory. Although in vitro protocols examining glucose transporter expression and glucose uptake by monocytes have been described, none have been examined by multi-parametric flow cytometry in whole blood...
2016: Journal of Visualized Experiments: JoVE
Ilvira M Khan, Yashashwi Pokharel, Razvan T Dadu, Dorothy E Lewis, Ron C Hoogeveen, Huaizhu Wu, Christie M Ballantyne
CONTEXT: Postprandial hyperlipidemia has been suggested to contribute to atherogenesis by inducing proinflammatory changes in monocytes. Individuals with metabolic syndrome (MS), shown to have higher blood triglyceride concentration and delayed triglyceride clearance, may thus have increased risk for development of atherosclerosis. OBJECTIVE: To examine fasting levels and effects of a high-fat meal on phenotypes of monocyte subsets in individuals with obesity and MS and in healthy controls...
August 30, 2016: Journal of Clinical Endocrinology and Metabolism
L Boff, I T Silva, D F Argenta, L M Farias, L F Alvarenga, R M Pádua, F C Braga, J P V Leite, J M Kratz, C M O Simões
AIMS: To investigate the anti-HSV and anti-inflammatory effects of a standardized ethyl acetate extract (SEAE) prepared with the stem bark of Strychnos pseudoquina, along with two isolated compounds: quercetin 3-O-methyl ether (3MQ) and strychnobiflavone (SBF). METHODS AND RESULTS: The mechanisms of action were evaluated by different methodological strategies. SEAE and SBF affected the early stages of viral infection and reduced HSV-1 protein expression. Both flavonoids elicited a concentration-dependent inhibition of monocyte chemoattractant protein-1 (MCP-1), whereas 3MQ reduced the chemokine release more significantly than SBF...
August 26, 2016: Journal of Applied Microbiology
Delphine Diana Acar, Dominique A J Olyslaegers, Annelike Dedeurwaerder, Inge D M Roukaerts, Wendy Baetens, Sebastiaan Van Bockstael, Gaëtan M A De Gryse, Lowiese M B Desmarets, Hans J Nauwynck
One of the most characteristic pathological changes in cats that have succumbed to feline infectious peritonitis (FIP) is a multifocal granulomatous phlebitis. Although it is now well established that leukocyte extravasation elicits the inflammation typically associated with FIP lesions, relatively few studies have aimed at elucidating this key pathogenic event. The upregulation of adhesion molecules on the endothelium is a prerequisite for stable leukocyte-endothelial cell (EC) adhesion that necessarily precedes leukocyte diapedesis...
August 19, 2016: Journal of General Virology
Šárka Lhoták, Gabriel Gyulay, Jean-Claude Cutz, Ali Al-Hashimi, Bernardo L Trigatti, Carl D Richards, Suleiman A Igdoura, Gregory R Steinberg, Jonathan Bramson, Kjetil Ask, Richard C Austin
BACKGROUND: Monocyte recruitment leads to accumulation of macrophage foam cells and contributes to atherosclerotic lesion growth. Recent studies have reported that lesion-resident macrophages can proliferate and represent a major cellular component during lesion development. This study was designed to assess whether the rate of macrophage proliferation changes during well-established stages of lesion growth and to characterize other populations of proliferating cells within these lesions...
2016: Journal of the American Heart Association
Clare Pridans, Gemma M Davis, Kristin A Sauter, Zofia M Lisowski, Yolanda Corripio-Miyar, Anna Raper, Lucas Lefevre, Rachel Young, Mary E McCulloch, Simon Lillico, Elspeth Milne, Bruce Whitelaw, David A Hume
Expression of Csf1r in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse Fms-intronic regulatory element and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate, and nonclassical monocytes in humans, based upon CD14 and CD16 expression...
September 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
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