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Intermediate monocyte

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https://www.readbyqxmd.com/read/28323396/toll-like-receptor-2-bright-cells-identify-circulating-monocytes-in-human-and-non-human-primates
#1
Erin N Shirk, Brian G Kral, Lucio Gama
Polychromatic flow cytometry is a useful tool for monitoring circulating whole blood monocytes, although gating strategies often vary depending on the study. Increased analyses of the myeloid system have revealed monocytes to be more plastic than previously understood and uncovered changes among surface markers previously considered to be stable. The myeloid system has also been found to have disparate surface markers between mouse, human, and non-human primate studies, which further complicates examination between species...
March 21, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28293233/the-soil-bacterium-methylococcus-capsulatus-bath-interacts-with-human-dendritic-cells-to-modulate-immune-function
#2
Stine Indrelid, Charlotte Kleiveland, René Holst, Morten Jacobsen, Tor Lea
The prevalence of inflammatory bowel disease (IBD) has increased in Western countries during the course of the twentieth century, and is evolving to be a global disease. Recently we showed that a bacterial meal of a non-commensal, non-pathogenic methanotrophic soil bacterium, Methylococcus capsulatus Bath prevents experimentally induced colitis in a murine model of IBD. The mechanism behind the effect has this far not been identified. Here, for the first time we show that M. capsulatus, a soil bacterium adheres specifically to human dendritic cells, influencing DC maturation, cytokine production, and subsequent T cell activation, proliferation and differentiation...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28281897/patients-with-acute-myeloid-leukemia-can-be-subclassified-based-on-the-constitutive-cytokine-release-of-the-leukemic-cells-the-possible-clinical-relevance-and-the-importance-of-cellular-iron-metabolism
#3
Annette K Brenner, Tor Henrik Anderson Tvedt, Ina Nepstad, Kristin P Rye, Karen M Hagen, Håkon Reikvam, Øystein Bruserud
OBJECTIVE: Acute myeloid leukaemia (AML) is a heterogeneous malignancy; we studied how the constitutive cytokine release by the AML cells varies among patients. METHODS: We investigated the constitutive release of 28 mediators during in vitro culture for 79 consecutive patients. RESULTS: Constitutive cytokine release profiles differed among patients, and hierarchical clustering identified three subsets with high, intermediate and low release, respectively...
April 2017: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/28255273/increase-in-peripheral-blood-intermediate-monocytes-is-associated-with-the-development-of-recent-onset-type-1-diabetes-mellitus-in-children
#4
Xiaoya Ren, Wenjun Mou, Chang Su, Xi Chen, Hui Zhang, Bingyan Cao, Xiaoqiao Li, Di Wu, Xin Ni, Jingang Gui, Chunxiu Gong
Monocytes play important roles in antigen presentation and cytokine production to achieve a proper immune response, and are therefore largely implicated in the development and progression of autoimmune diseases. The aim of this study was to analyze the change in the intermediate (CD14+CD16+) monocyte subset in children with recent-onset type 1 diabetes mellitus (T1DM) and its possible association with clinical parameters reflecting islet β-cell dysfunction. Compared with age- and sex-matched healthy controls, intermediate monocytes were expanded in children with T1DM, which was positively associated with hemoglobin A1C and negatively associated with serum insulin and C-peptide...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28250118/imaging-hiv-1-genomic-dna-from-entry-through-productive-infection
#5
Ryan D Stultz, Jennifer J Cenker, David McDonald
In order to track the fate of HIV-1 particles from early entry events through productive infection, we developed a method to visualize HIV-1 DNA reverse transcription complexes by incorporation and fluorescent labeling of the thymidine analog 5-ethynyl-2' -deoxyuridine (EdU) into nascent viral DNA during cellular entry. Monocyte-derived macrophages were chosen as natural targets of HIV-1 which do not divide and therefore do not incorporate the EdU into chromosomal DNA, which would obscure detection of intranuclear HIV-1 genomes...
March 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28239800/intermediate-cd14-cd16-monocyte-predicts-severe-coronary-stenosis-and-extensive-plaque-involvement-in-asymptomatic-individuals
#6
Shyh-Chyi Lo, Wen-Jeng Lee, Ching-Yi Chen, Bai-Chin Lee
Circulating leukocyte subtypes and monocyte subsets are independent predictors of cardiovascular events. We hypothesized that an increased leukocyte subtype would predict severe coronary stenosis and extensive plaque involvement. We retrospectively analyzed clinical, laboratory, and coronary CT data in a total of 588 asymptomatic adults (69% men; mean age, 57 ± 9 years) undergoing a general health check-up. Intermediate CD14(++)CD16(+) monocyte count had the strongest association with mixed and calcified plaque scores, whereas the numbers of neutrophils and classical CD14(++)CD16(-) monocytes were significantly associated with non-calcified plaque score...
February 26, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28229168/cd14-cd16-nonclassical-monocytes-are-associated-with-endothelial-dysfunction-in-patients-with-coronary-artery-disease
#7
Karol Urbanski, Dominik Ludew, Grzegorz Filip, Magdalena Filip, Agnieszka Sagan, Piotr Szczepaniak, Grzegorz Grudzien, Jerzy Sadowski, Barbara Jasiewicz-Honkisz, Tomasz Sliwa, Boguslaw Kapelak, Eilidh McGinnigle, Tomasz Mikolajczyk, Tomasz J Guzik
Endothelial dysfunction and inflammation are key mechanisms of vascular disease. We hypothesised that heterogeneity of monocyte subpopulations may be related to the development of vascular dysfunction in coronary artery disease (CAD). Therefore, we examined the relationships between monocyte subsets (CD14(++)CD16(-) "classical - Mon1", CD14(++)CD16(+) "intermediate - Mon2" and CD14(+)CD16(++) "nonclassical - Mon3"), endothelial function and risk factor profiles in 130 patients with CAD undergoing coronary artery bypass grafting...
February 23, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28228523/vpx-overcomes-a-samhd1-independent-block-to-hiv-reverse-transcription-that-is-specific-to-resting-cd4-t-cells
#8
Hanna-Mari Baldauf, Lena Stegmann, Sarah-Marie Schwarz, Ina Ambiel, Maud Trotard, Margarethe Martin, Manja Burggraf, Gina M Lenzi, Helena Lejk, Xiaoyu Pan, Oliver I Fregoso, Efrem S Lim, Libin Abraham, Laura A Nguyen, Frank Rutsch, Renate König, Baek Kim, Michael Emerman, Oliver T Fackler, Oliver T Keppler
Early after entry into monocytes, macrophages, dendritic cells, and resting CD4 T cells, HIV encounters a block, limiting reverse transcription (RT) of the incoming viral RNA genome. In this context, dNTP triphosphohydrolase SAM domain and HD domain-containing protein 1 (SAMHD1) has been identified as a restriction factor, lowering the concentration of dNTP substrates to limit RT. The accessory lentiviral protein X (Vpx) proteins from the major simian immunodeficiency virus of rhesus macaque, sooty mangabey, and HIV-2 (SIVsmm/SIVmac/HIV-2) lineage packaged into virions target SAMHD1 for proteasomal degradation, increase intracellular dNTP pools, and facilitate HIV cDNA synthesis...
March 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28223716/preoperative-red-cell-distribution-width-and-neutrophil-to-lymphocyte-ratio-predict-survival-in-patients-with-epithelial-ovarian-cancer
#9
Zheng Li, Na Hong, Melissa Robertson, Chen Wang, Guoqian Jiang
Several parameters of preoperative complete blood count (CBC) and inflammation-associated blood cell markers derived from them have been reported to correlate with prognosis in patients with epithelial ovarian cancer (EOC), but their prognostic importance and optimal cutoffs are still needed be elucidated. Clinic/pathological parameters, 5-year follow-up data and preoperative CBC parameters were obtained retrospectively in 654 EOC patients underwent primary surgery at Mayo Clinic. Cutoffs for neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and monocyte-to-lymphocyte ratio (MLR) were optimized by receiver operating characteristic (ROC) curve...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28222802/counts-of-bovine-monocyte-subsets-prior-to-calving-are-predictive-for-postpartum-occurrence-of-mastitis-and-metritis
#10
Brianna Pomeroy, Anja Sipka, Jamal Hussen, Melanie Eger, Ynte Schukken, Hans-Joachim Schuberth
The heightened susceptibility to infectious diseases in postpartum dairy cows is often attributed to immune dysfunction associated with the transition period. However, the cell populations involved in this immune dysfunction and the dynamics between those populations are not well defined. Monocytes play a crucial role in governing initial immune response in bacterial infections. Bovine monocytes are subdivided in classical (CD14(+)/CD16(-)), intermediate (CD14(+)/CD16(+)) and non-classical monocytes (CD14(-)/CD16(+)) with distinct phenotypic and functional differences...
February 21, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28193827/monocyte-subsets-coregulate-inflammatory-responses-by-integrated-signaling-through-tnf-and-il-6-at-the-endothelial-cell-interface
#11
Myriam Chimen, Clara M Yates, Helen M McGettrick, Lewis S C Ward, Matthew J Harrison, Bonita Apta, Lea H Dib, Beat A Imhof, Paul Harrison, Gerard B Nash, G Ed Rainger
Two major monocyte subsets, CD14(+)CD16(-) (classical) and CD14(+/dim)CD16(+) (nonclassical/intermediate), have been described. Each has different functions ascribed in its interactions with vascular endothelial cells (EC), including migration and promoting inflammation. Although monocyte subpopulations have been studied in isolated systems, their influence on EC and on the course of inflammation has been ignored. In this study, using unstimulated or cytokine-activated EC, we observed significant differences in the recruitment, migration, and reverse migration of human monocyte subsets...
April 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28183821/exercise-induced-upregulation-of-endothelial-adhesion-molecules-in-human-skeletal-muscle-and-number-of-circulating-cells-with-remodeling-properties
#12
Anna Strömberg, Eric Rullman, Eva Jansson, Thomas Gustafsson
Multipotent cells have received great interest due to their potential capacity to repair and remodel peripheral tissues. We examined the effect of an acute exercise bout on the number of circulating cells with known remodeling properties, and the level of factors in plasma and skeletal muscle tissue with potential to recruit these cells. Twenty healthy male subjects performed a 60 min cycling exercise. Blood samples for flow cytometry were drawn from 10 subjects (Group 1) before and up to 2 h after exercise, and absolute cell counts of the classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)), and nonclassical (CD14(+)CD16(++)) monocyte (MO) subpopulations, and of CD45(dim)CD34(+)VEGFR2(+) endothelial progenitor cells (EPCs) were measured using bead-based determination...
February 9, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28183329/cd14-bright-cd16-intermediate-monocytes-are-induced-by-interleukin-10-and-positively-correlate-with-disease-activity-in-rheumatoid-arthritis
#13
Masako Tsukamoto, Noriyuki Seta, Keiko Yoshimoto, Katsuya Suzuki, Kunihiro Yamaoka, Tsutomu Takeuchi
BACKGROUND: Three different subsets of circulating human monocytes, CD14(bright)CD16- (classical), CD14(bright)CD16+ (intermediate), and CD14(dim)CD16+ (non-classical) have been recently identified. It has been reported that CD14(bright)CD16+ monocytes are increased in rheumatoid arthritis (RA). However, the role of each monocyte subset in the pathogenesis of RA is still unclear. The purpose of this study was to investigate the association of CD14(bright)CD16+ monocytes with RA. METHODS: The study enrolled 35 patients with RA and 14 healthy volunteers...
February 10, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28176950/circulating-monocytes-and-b-lymphocytes-in-neovascular-age-related-macular-degeneration
#14
Sven Magnus Hector, Torben Lykke Sørensen
BACKGROUND: Individuals with neovascular age-related macular degeneration (AMD) have altered number and distribution of retinal macrophages and show changes in circulating antibodies. We wanted to investigate the corresponding precursors, with subpopulations. We therefore measured monocyte and B-lymphocyte populations in individuals with neovascular AMD. DESIGN: This was an observational case-control study. PARTICIPANTS OR SAMPLES: A total of 31 individuals with neovascular AMD and 30 healthy age-matched controls were included...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28102045/inflammatory-profile-discriminates-clinical-subtypes-in-lrrk2-associated-parkinson-s-disease
#15
K Brockmann, C Schulte, N Schneiderhan-Marra, A Apel, C Pont-Sunyer, D Vilas, J Ruiz-Martinez, M Langkamp, J-C Corvol, F Cormier, T Knorpp, T O Joos, A Bernard, T Gasser, C Marras, B Schüle, J O Aasly, T Foroud, J F Marti-Masso, A Brice, E Tolosa, D Berg, W Maetzler
BACKGROUND AND PURPOSE: The presentation of Parkinson's disease patients with mutations in the LRRK2 gene (PDLRRK2 ) is highly variable, suggesting a strong influence of modifying factors. In this context, inflammation is a potential candidate inducing clinical subtypes. METHODS: An extensive battery of peripheral inflammatory markers was measured in human serum in a multicentre cohort of 142 PDLRRK2 patients from the MJFF LRRK2 Consortium, stratified by three different subtypes as recently proposed for idiopathic Parkinson's disease: diffuse/malignant, intermediate and mainly pure motor...
February 2017: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
https://www.readbyqxmd.com/read/28097522/severe-aortic-valve-stenosis-in-adults-is-associated-with-increased-levels-of-circulating-intermediate-monocytes
#16
Bernd Hewing, Sebastian Chi-Diep Au, Antje Ludwig, Rena Ellerbroek, Phillip van Dijck, Lisa Hartmann, Herko Grubitzsch, Carolin Giannini, Michael Laule, Verena Stangl, Gert Baumann, Karl Stangl
Individual monocyte subsets have been associated with atherosclerotic disease, but their distribution has not been evaluated in aortic valve stenosis (AS) so far. In the present study, we have asked whether levels of the circulating intermediate monocyte subset are increased in AS. Classical (CD14++CD16-), intermediate (CD14++CD16+), and non-classical (CD14+CD16++) CD86-positive monocytes and monocyte activation (intensity of CD11b expression) were determined by flow cytometry in peripheral blood of patients with severe AS (n = 100) and matched AS-free controls (n = 75)...
February 2017: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/28095688/effect-of-gold-nanoparticle-size-and-coating-on-labeling-monocytes-for-ct-tracking
#17
Peter Chhour, Johoon Kim, Barbara Benardo, Alfredo Tovar, Shaameen Mian, Harold I Litt, Victor A Ferrari, David P Cormode
With advances in cell therapies, interest in cell tracking techniques to monitor the migration, localization, and viability of these cells continues to grow. X-ray computed tomography (CT) is a cornerstone of medical imaging but has been limited in cell tracking applications due to its low sensitivity toward contrast media. In this study, we investigate the role of size and surface functionality of gold nanoparticles for monocyte uptake to optimize the labeling of these cells for tracking in CT. We synthesized gold nanoparticles (AuNP) that range from 15 to 150 nm in diameter and examined several capping ligands, generating 44 distinct AuNP formulations...
January 18, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28044051/maturation-phenotype-of-peripheral-blood-monocyte-macrophage-after-stimulation-with-lipopolysaccharides-in-irritable-bowel-syndrome
#18
Oscar A Rodríguez-Fandiño, Joselín Hernández-Ruiz, Yolanda López-Vidal, Luis Charúa-Guindic, Galileo Escobedo, Max J Schmulson
Background/Aims: Abnormal immune regulation and increased intestinal permeability augmenting the passage of bacterial molecules that can activate immune cells, such as monocytes/macrophages, have been reported in irritable bowel syndrome (IBS). To compare the maturation phenotype of monocytes/macrophages (CD14+) from IBS patients and controls in the presence or absence of E. coli lipopolysaccharides (LPS), in vitro. Methods: Mononuclear cells were isolated from peripheral blood of 20 Rome II-IBS patients and 19 controls and cultured with or without LPS for 72 hours...
January 2, 2017: Journal of Neurogastroenterology and Motility
https://www.readbyqxmd.com/read/28039482/preoperative-pulmonary-function-correlates-with-systemic-inflammatory-response-and-prognosis-in-patients-with-non-small-cell-lung-cancer-results-of-a-single-institution-retrospective-study
#19
Yongyin Gao, Hongdian Zhang, Yue Li, Dandan Wang, Yinlu Ma, Qing Chen
This study aimed at analyzing the relationship between preoperative pulmonary function and systemic inflammatory response (SIR) biomarkers, such as neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and lymphocyte-to-monocyte ratio (LMR) in patients with non-small cell lung cancer (NSCLC). Furthermore, the prognostic significance of these markers was also examined. The medical records of 358 NSCLC patients, who underwent curative lung resection, were retrospectively analyzed. Pulmonary function test values <80% of the predicted values were used to indicate impairment...
December 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27992874/differential-expression-of-lipoprotein-associated-phospholipase-a2-in-monocyte-subsets-impact-of-uremia-and-atherosclerosis
#20
Christof Ulrich, Bogusz Trojanowicz, Roman Fiedler, Felix Kohler, Anna-Franziska Wolf, Eric Seibert, Matthias Girndt
BACKGROUND: Monocytic products, such as lipoprotein-associated phospholipase A2 (Lp-PLA2), may participate in the development of atherosclerosis. Heterogeneity of monocytes is widely acknowledged. Classical, intermediate, and non-classical subsets can be discerned. Recently, an inflammatory, pro-atherogenic monocyte population could be identified in hemodialysis patients. In this study, we investigated the expression of Lp-PLA2 on leucocytes and different monocyte subpopulations and their possible role in uremia, inflammation, and atherosclerosis...
2017: Nephron
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