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lung cancer rna

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https://www.readbyqxmd.com/read/28529576/increased-mir31hg-lncrna-expression-increases-gefitinib-resistance-in-non-small-cell-lung-cancer-cell-lines-through-the-egfr-pi3k-akt-signaling-pathway
#1
Bing Wang, Hong Jiang, Limin Wang, Xueqin Chen, Kan Wu, Shirong Zhang, Shenglin Ma, Bing Xia
The aim of the present study was to gain insight into the molecular mechanism of gefitinib resistance in non-small cell lung cancer (NSCLC), and demonstrate whether long noncoding RNA (lncRNA) expression signatures differ between gefitinib-sensitive PC9 and gefitinib-resistant PC9 (PC9-R) cell lines. PC9 and PC9-R cells were treated with gefitinib and, after 48 h, proliferation and apoptosis were analyzed using a Cell Counting Kit-8 (CCK-8) assay and flow cytometry. Microarray expression profiling of lncRNAs was undertaken in both PC9 and PC9-R cells, and the expression profiles were verified by reverse transcription quantitative-polymerase chain reaction...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28529241/downregulation-of-transcription-factor-sp1-suppresses-malignant-properties-of-a549-human-lung-cancer-cell-line-with-decreased-%C3%AE-4-galactosylation-of-highly-branched-n-glycans
#2
Kodai Muramoto, Riho Tange, Takayuki Ishii, Kana Miyauchi, Takeshi Sato
Dramatic changes in the glycan structures of cell surface proteins have been observed upon malignant transformation of cells as induced by the altered expression levels of glycosyltransferases. Such changes are closely associated with the malignant properties of cancer cells. Transcription factor Sp1 regulates the gene expression of various molecules including glycosyltransferases. Herein, we investigated whether or not Sp1-downregulation affects to N-glycosylation of glycoproteins and malignant properties of A549 human lung carcinoma cell line...
May 20, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28527113/mutant-p53-promotes-cell-spreading-and-migration-via-arhgap44
#3
Jinjin Xu, Jian Jiao, Wei Xu, Lei Ji, Dongjie Jiang, Shaofang Xie, Syeda Kubra, Xiaotao Li, Junjiang Fu, Jianru Xiao, Bianhong Zhang
The tumor suppressor p53 protein is either lost or mutated in about half of all human cancers. Loss of p53 function is well known to influence cell spreading, migration and invasion. While expression of mutant p53 is not equivalent to p53 loss, mutant p53 can acquire new functions to drive cell spreading and migration via different mechanisms. In our study, we found that mutant p53 significantly increased cell spreading and migration when comparing with p53-null cells. RNA-Seq analysis suggested that Rho GTPase activating protein 44 (ARHGAP44) is a new target of mutant p53, which suppressed ARHGAP44 transcription...
May 16, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28523597/prognostic-value-of-chemotherapy-resistant-ck19mrna-positive-circulating-tumor-cells-in-patients-with-advanced-metastatic-non-small-cell-lung-cancer
#4
G Milaki, I Messaritakis, F Koinis, A Kotsakis, S Apostolaki, E K Dermitzaki, M Perraki, D Hatzidaki, V Georgoulias
BACKGROUND: The detection of circulating tumor cells (CTCs) is of prognostic significance in several tumor types. The present study evaluated the detection and the clinical relevance of CK19mRNA(+) CTCs in patients with advanced/metastatic non-small cell lung cancer before and after front-line chemotherapy. PATIENTS AND METHODS: Peripheral blood was obtained from 642 patients with treatment-naïve unresectable stage IIIB and IV non-small cell lung cancer and from 455 patients after the completion of 1st line chemotherapy...
May 18, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28522900/high-throughput-rna-sequencing-utility-for-diagnosis-and-prognosis-in-colon-diseases
#5
EDITORIAL
Mamie Gao, Allen Zhong, Neil Patel, Chiraag Alur, Dinesh Vyas
RNA sequencing is the use of high throughput next generation sequencing technology to survey, characterize, and quantify the transcriptome of a genome. RNA sequencing has been used to analyze the pathogenesis of several malignancies such melanoma, lung cancer, and colorectal cancer. RNA sequencing can identify differential expression of genes (DEG's), mutated genes, fusion genes, and gene isoforms in disease states. RNA sequencing has been used in the investigation of several colorectal diseases such as colorectal cancer, inflammatory bowel disease (ulcerative colitis and Crohn's disease), and irritable bowel syndrome...
April 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28521440/t-box-3-overexpression-is-associated-with-poor-prognosis-of-non-small-cell-lung-cancer
#6
Yueming Wu, Jiang Feng, Weiwei Hu, Yawei Zhang
T-box 3 (Tbx3), a member of the T-box transcription factor family, serves a crucial role in embryonic development and cancer progression. Previous studies have demonstrated the clinical significance of Tbx3 in numerous types of cancer. However, the expression level and pathological function of Tbx3 in non-small cell lung cancer (NSCLC) are unknown. To the best of our knowledge, the present study provided the first evidence demonstrating the clinicopathological significance of Tbx3 in NSCLC. Tbx3 was revealed to be overexpressed in NSCLC cell lines and tissues obtained from patients with NSCLC by reverse transcription-quantitative polymerase chain reaction and western blot analysis...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28521430/reduced-expression-levels-of-pten-are-associated-with-decreased-sensitivity-of-hcc827-cells-to-icotinib
#7
Yang Zhai, Yanjun Zhang, Kejun Nan, Xuan Liang
The clinical resistance of non-small cell lung cancer (NSCLC) to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) has been linked to EGFR T790M resistance mutations or MET amplifications. Additional mechanisms underlying EGFR-TKI drug resistance remain unclear. The present study demonstrated that icotinib significantly inhibited the proliferation and increased the apoptosis rate of HCC827 cells; the cellular mRNA and protein expression levels of phosphatase and tensin homolog (PTEN) were also significantly downregulated...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28521411/reduced-rkip-expression-levels-are-associated-with-frequent-non-small-cell-lung-cancer-metastasis-and-stat3-phosphorylation-and-activation
#8
Ansheng Wang, Guixin Duan, Chengling Zhao, Yuan Gao, Xuegang Liu, Zuyi Wang, Wei Li, Kangwu Wang, Wei Wang
The current study examined the role of Raf kinase inhibitor protein (RKIP) in non-small cell lung cancer (NSCLC) metastasis. A total of 100 patients with NSCLC were recruited following pathological diagnosis in the First Affiliated Hospital of Bengbu Medical College. The patients were classified and statistically analyzed according to their clinicopathological characteristics and tumor-node-metastasis stage. Paired tumor tissue and adjacent non-tumor tissue samples were subject to pathological diagnosis and western blot analysis...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28515040/a-systematic-review-of-overlapping-microrna-patterns-in-systemic-sclerosis-and-idiopathic-pulmonary-fibrosis
#9
REVIEW
Gianluca Bagnato, William Neal Roberts, Jesse Roman, Sebastiano Gangemi
Lung fibrosis can be observed in systemic sclerosis and in idiopathic pulmonary fibrosis, two disorders where lung involvement carries a poor prognosis. Although much has been learned about the pathogenesis of these conditions, interventions capable of reversing or, at the very least, halting disease progression are not available. Recent studies point to the potential role of micro messenger RNAs (microRNAs) in cancer and tissue fibrogenesis. MicroRNAs are short non-coding RNA sequences (20-23 nucleotides) that are endogenous, evolutionarily conserved and encoded in the genome...
June 30, 2017: European Respiratory Review: An Official Journal of the European Respiratory Society
https://www.readbyqxmd.com/read/28514759/charge-reversible-calcium-phosphate-lipid-hybridnanoparticle-for-sirna-delivery
#10
Rong-Qiao Cai, Dao-Zhou Liu, Han Cui, Ying Cheng, Miao Liu, Bang-Le Zhang, Qi-Bing Mei, Si-Yuan Zhou
Bcl-2 gene is an important target to treat lung cancer. The small interference RNA (siRNA) of Bcl-2 gene (siBcl-2) can specifically silence Bcl-2 gene. However, naked siBcl-2 is difficult to accumulate in the tumor tissue to exert its activity. In this paper, a calcium phosphate lipid hybrid nanoparticle that possessed charge reversible property was prepared to enhance the activity of siBcl-2 in vivo. The average diameter and zeta potential of siBcl-2 loaded calcium phosphate lipid hybrid nanoparticles (LNPS@siBcl-2) were 80 nm and -13 mV at pH7...
April 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28510306/a-polymorphism-in-mir-1262-regulatory-region-confers-the-risk-of-lung-cancer-in-chinese-population
#11
Kaipeng Xie, Mengxi Chen, Meng Zhu, Cheng Wang, Na Qin, Cheng Liang, Ci Song, Juncheng Dai, Guangfu Jin, Hongbing Shen, Dongxing Lin, Hongxia Ma, Zhibin Hu
It has been proposed that the majority of disease-associated loci identified by genome-wide association studies (GWAS) are enriched in non-coding regions, such as the promoter, enhancer or non-coding RNA genes. Thus, we performed a two-stage case-control study to systematically evaluate the association of genetic variants in miRNA regulatory regions (promoter and enhancer) with lung cancer risk in 7,763 subjects (discovery stage: 2,331 cases and 3,077 controls; validation stage: 1,065 cases and 1,290 controls)...
May 16, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28510291/associations-between-rna-splicing-regulatory-variants-of-stemness-related-genes-and-racial-disparities-in-susceptibility-to-prostate-cancer
#12
Yanru Wang, Jennifer A Freedman, Hongliang Liu, Patricia G Moorman, Terry Hyslop, Daniel J George, Norman H Lee, Steven R Patierno, Qingyi Wei
Evidence suggests that cells with a stemness phenotype play a pivotal role in oncogenesis, and prostate cells exhibiting this phenotype have been identified. We used two genome-wide association study (GWAS) datasets of African descendants, from the Multiethnic/Minority Cohort Study of Diet and Cancer (MEC) and the Ghana Prostate Study, as well as two GWAS datasets of non-Hispanic whites, from the Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Trial and the Breast and Prostate Cancer Cohort Consortium (BPC3), to analyze the associations between genetic variants of stemness-related genes and racial disparities in susceptibility to prostate cancer...
May 16, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28509438/deguelin-inhibits-epithelial-to-mesenchymal-transition-and-metastasis-of-human-non-small-cell-lung-cancer-cells-by-regulating-nima-related-kinase-2
#13
Dejian Zhao, Wenzheng Han, Xia Liu, Dawei Cui, Yu Chen
BACKGROUND: Non-small cell lung cancer is a lethal malignancy with a high mortality rate. Deguelin displays an anti-tumor effect and inhibits metastasis in various cancers. The aberrant expression of NIMA-related kinase 2 (NEK2) indicates poor prognosis and induces epithelial-to-mesenchymal transition (EMT) and metastasis processes. However, the underlying mechanism between deguelin and NEK2 has remained elusive. METHODS: NSCLC cell lines were treated with deguelin...
May 16, 2017: Thoracic Cancer
https://www.readbyqxmd.com/read/28501870/long-non-coding-rna-xloc_008466-functions-as-an-oncogene-in-human-non-small-cell-lung-cancer-by-targeting-mir-874
#14
Rui Yang, Ping Li, Guojun Zhang, Chunya Lu, Huaqi Wang, Guoqiang Zhao
BACKGROUND: The therapy and prognosis of lung cancer are difficult because of multiple genetic and epigenetic alterations. Long non-coding RNAs (lncRNAs) have been verified as new mediators of cancer development and progression by virtue of their various functions. Here, we focused on the lncRNA XLOC_008466 based on previous microarray data. However, whether aberrant expression of XLOC_008466 in human non-small cell lung cancer (NSCLC) is correlated with malignancy, metastasis or prognosis has not been elucidated...
May 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28501728/effects-of-fine-air-particulates-on-gene-expression-in-non-small-cell-lung-cancer
#15
Biao Yang, Xinming Li, Dongmei Chen, Chunling Xiao
PURPOSE: Airborne particulate matter smaller than 2.5μm (PM2.5) has been shown to induce adverse health effects through various mechanisms. However, its effects on gene expression in non-small-cell lung cancer (NSCLC) remain undefined. The aim of this study was to analyze the expression profile of PM2.5-induced adverse health effects on human. MATERIALS AND METHODS: We performed RNA sequencing to elucidate key molecular effects of PM2.5 collected from Shenyang China, to identify potential diagnostic markers or therapeutic targets, and further validated these differences in gene expression by using quantitative PCR in A549 and H1299 human non-small-cell lung cancer cell lines...
May 11, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28500171/platelet-microparticles-infiltrating-solid-tumors-transfer-mirnas-that-suppress-tumor-growth
#16
James V Michael, Jeremy G T Wurtzel, Guang Fen Mao, A Koneti Rao, Mikhail A Kolpakov, Abdelkarim Sabri, Nicholas E Hoffman, Sudarsan Rajan, Dhanendra Tomar, Muniswamy Madesh, Marvin T Nieman, Johnny Yu, Leonard C Edelstein, Jesse W Rowley, Andrew S Weyrich, Lawrence E Goldfinger
Platelet-derived microparticles (PMPs) are associated with enhancement of metastasis and poor cancer outcomes. Circulating PMPs transfer platelet microRNAs (miRNAs) to vascular cells. Solid tumor vasculature is highly permeable, allowing the possibility of PMP-tumor cell interaction. Here we show that PMPs infiltrate solid tumors in humans and mice and transfer platelet-derived RNA, including miRNAs, to tumor cells in vivo and in vitro, resulting in tumor cell apoptosis. MiR-24 was a major species in this transfer...
May 12, 2017: Blood
https://www.readbyqxmd.com/read/28498804/increased-s100a15-expression-and-decreased-dna-methylation-of-its-gene-promoter-are-involved-in-high-metastasis-potential-and-poor-outcome-of-lung-adenocarcinoma
#17
Yung-Che Chen, Meng-Chih Lin, Chang-Chun Hsiao, Yi-Xin Zheng, Kuang-Den Chen, Ming-Tse Sung, Chung-Jen Chen, Ting-Ya Wang, Yong-Yong Lin, Huang-Chih Chang, Yu-Mu Chen, Jen-Chieh Chang
PURPOSE: This study aims to determine the functional role of S100A15 and its promoter DNA methylation patterns in lung cancer progression. EXPERIMENTAL DESIGN: We analyzed 178 formalin-fixed paraffin embedded specimens from lung cancer patients, including 24 early stage and 91 advanced stage adenocarcinoma. S100A15 protein expression was evaluated by immunohistochemistry stain, and its DNA methylation levels were measured by pyrosequencing. RESULTS: S100A15 nuclear staining was increased in lung adenocarcinoma patients with distant metastasis versus those without distant metastasis...
April 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28498474/effect-of-mir-1244-on-cisplatin-treated-non-small-cell-lung-cancer-via-mef2d-expression
#18
Guang-Jian Li, Guang-Qiang Zhao, Jia-Peng Yang, Yong-Chun Zhou, Kai-Yun Yang, Yu-Jie Lei, Yun-Chao Huang
The aim of this study was to investigate the function of miR-1244 in cisplatin-treated non-small cell lung cancer (NSCLC). The results of quantitative PCR analysis revealed that the expression levels of miR-1244 in cisplatin‑treated A549 and NCI-H522 human lung cancer cell lines were lower than those in untreated A549 and NCI-H522 cells. Similarly, the expression level of miR-1244 in NSCLC tissue samples from cisplatin-treated patients was also lower than that in non-cisplatin-treated NSCLC patients. Notably, the overall survival times of cisplatin-treated NSCLC patients with high miR-1244 expression were superior to those patients with low miR-1244 expression...
May 4, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28498455/overexpression-of-magea2-has-a-prognostic-significance-and-is-a-potential-therapeutic-target-for-patients-with-lung-cancer
#19
Hideki Ujiie, Tatsuya Kato, Daiyoon Lee, Hsin-Pei Hu, Kosuke Fujino, Mitsuhito Kaji, Kichizo Kaga, Yoshiro Matsui, Kazuhiro Yasufuku
Melanoma-associated antigens (MAGE) are expressed in different type of cancers including lung cancer and have been shown to be functionally related to p53 tumor suppressor gene. Little is known about the relationship between MAGE genes and p53 aberrant expression in lung cancer. The aims of this study were to observe the expression of MAGEA2, examine the role of MAGEA2 in lung cancer survival, investigate its correlation between MAGEA2 and p53, and explore its clinicopathologic significance as a prognostic marker...
May 5, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28498425/gene-silencing-of-indoleamine-2-3-dioxygenase-hinders-tumor-growth-through-angiogenesis-inhibition
#20
Jinfeng Pan, Keng Yuan, Shanshan Peng, Yanqin Huang, Yujuan Zhang, Yinying Hu, Yuanyuan Feng, Yanmei Shi, Yanling Liu, Hongmei Wang, Nanjin Zhou, Weiping Min
The significance of indoleamine 2,3-dioxygenase-1 (IDO1) has been studied in various types of tumors, but the relationship between IDO1 and tumor angiogenesis needs further delineation. We aimed to clarify the relationship between tumor angiogenesis and IDO1 expression, and to explore the possibility of IDO1-targeting molecular therapy for lung cancer. For the first time, we found that silencing the IDO1 gene using small interfering RNA (siRNA) inhibits in vitro cancer cell invasion and migration. We further demonstrated that knockdown of IDO1 decreased the formation of vasculogenic mimicry...
April 25, 2017: International Journal of Oncology
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