Rei-Ichi Ohno, Kenta Ichimaru, Seitaro Tanaka, Hikari Sugawa, Nana Katsuta, Shiori Sakake, Yu-Ki Tominaga, Ikuho Ban, Jun-Ichi Shirakawa, Yoshiki Yamaguchi, Emi Ito, Naoyuki Taniguchi, Ryoji Nagai
Prolonged hyperglycemia generates advanced glycation end-products (AGEs), which are believed to be involved in the pathogenesis of diabetic complications. In the present study, we developed a polyclonal antibody against fructose-modified proteins (Fru-P antibody) and identified its epitope as glucoselysine (GL) by NMR and LC- electrospray ionization (ESI)- quadrupole TOF (QTOF) analyses and evaluated its potential role in diabetes sequelae. Although the molecular weight of GL was identical to that of fructoselysine (FL), GL was distinguishable from FL because GL was resistant to acid hydrolysis, which converted all of the FLs to furosine...
October 8, 2019: Journal of Biological Chemistry