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Laryngeal carcinoma and radioresistance

Han Lei, Dali Feng, Fuxiang Zhou, Hui Xu, Tian Tang, Haijun Yu, Conghua Xie, Yunfeng Zhou
The close association between telomere length and radiosensitivity has been established by several studies. There is also a hypothesis that telomere length may be regulated by human protection of telomere 1 (hPOT1) in human carcinoma cells. In the present study, the hPOT1 level between the radioresistant Hep-2R cells and the wild-type were compared, and the results showed that the hPOT1 gene was upregulated in the radioresistant Hep-2R cell lines compared with the wild-type. This suggested that the expression level of hPOT1 correlates with radiosensitivity...
August 2015: Oncology Letters
Xing-Mei Luo, Bin Xu, Min-Li Zhou, Yang-Yang Bao, Shui-Hong Zhou, Jun Fan, Zhong-Jie Lu
In the present study, we investigated the role of GLUT-1 and PI3K/Akt signaling in radioresistance of laryngeal carcinoma xenografts. Volume, weight, radiosensitization, and the rate of inhibition of tumor growth in the xenografts were evaluated in different groups. Apoptosis was evaluated by TUNEL assay. In addition, mRNA and protein levels of GLUT-1, p-Akt, and PI3K in the xenografts were measured. Treatment with LY294002, wortmannin, wortmannin plus GLUT-1 AS-ODN, and LY294002 plus GLUT-1 AS-ODN after X-ray irradiation significantly reduced the size and weight of the tumors, rate of tumor growth, and apoptosis in tumors compared to that observed in the 10-Gy group (p<0...
2015: PloS One
Yusuke Demizu, Osamu Fujii, Fumiko Nagano, Kazuki Terashima, Dongcun Jin, Masayuki Mima, Naoharu Oda, Kaoru Takeuchi, Makiko Takeda, Kazuyuki Ito, Nobukazu Fuwa, Tomoaki Okimoto
Carbon ion therapy is a type of radiotherapy that can deliver high-dose radiation to a tumor while minimizing the dose delivered to organs at risk. Moreover, carbon ions are classified as high linear energy transfer radiation and are expected to be effective for even photon-resistant tumors. A 73-year-old man with glottic squamous cell carcinoma, T3N0M0, refused laryngectomy and received carbon ion therapy of 70 Gy (relative biological effectiveness) in 35 fractions. Three months after the therapy, the patient had an upper airway inflammation, and then laryngeal edema and pain occurred...
November 2015: Japanese Journal of Clinical Oncology
Danielle Maia, Ana Carolina de Carvalho, Maria Aderuza Horst, André Lopes Carvalho, Cristovam Scapulatempo-Neto, Andre Luiz Vettore
PURPOSE: Definitive radiation therapy is the mainstay of treatment for early stage laryngeal squamous cell carcinoma (LSCC). However, up to 30% of the patients do not respond to radiotherapy. Unfortunately, we are unable to predict which tumors are likely to respond to radiation, and which will be resistant and persist. Therefore, the development of novel markers to predict response to radiotherapy is urgently needed. This study was designed to evaluate the expression pattern of microRNAs (miRNAs) in LSCC in order to identify markers capable of segregating radioresistant and radiosensitive tumors and to investigate the relationship between the expression of these miRNAs and the prognosis of LSCC...
2015: Journal of Translational Medicine
Yang-Yang Bao, Shui-Hong Zhou, Zhong-Jie Lu, Jun Fan, Ya-Ping Huang
Hypoxia is an important factor in radioresistance of laryngeal carcinoma. Glucose transporter-1 (GLUT-1) is an important hypoxic marker in malignant tumors, including laryngeal carcinoma. Apigenin is a natural phytoestrogen flavonoid that has potential anticancer effects. Various studies have reported that the effects of apigenin on lowering GLUT-1 expression were involved in downregulation of the PI3K/Akt pathway. Thus, apigenin may improve the radiosensitivity of laryngeal carcinoma by suppressing the expression of GLUT-1 via the PI3K/Akt pathway...
October 2015: Oncology Reports
Li Xu, Zhifeng Chen, Fei Xue, Wei Chen, Ruina Ma, Shiyin Cheng, Pengcheng Cui
BACKGROUND: Increasing evidence indicates that dysregulation of microRNAs is involved in tumor progression and development. The aim of this study was to investigate the expression of microRNA-24 (miR-24) and its function in laryngeal squamous cell carcinoma (LSCC). METHODS: Quantitative RT-PCR (qRT-PCR) was used to detect miR-24 expression in LSCC cell lines and tissue samples. MTT, colony formation, and flow cytometry was performed to analyze the effects of miR-24 expression on growth, apoptosis, and radiosensitivity of LSCC cells...
2015: Cancer Cell International
Jae-Sung Kim, Sun Young Kim, Minyoung Lee, Sung Hyun Kim, Sang-Man Kim, Eun Ju Kim
Accumulating evidence suggests that changes in methylation patterns may help mediate the sensitivity or resistance of cancer cells to ionizing radiation. The present study provides evidence for the involvement of radioresistance-induced DNA methylation changes in tumor radioresistance. We established radioresistant laryngeal cancer cells via long-term fractionated irradiation, and examined differences in DNA methylation between control and radioresistant laryngeal cancer cells. Interestingly, we found that the promoter-CpG islands of 5 previously identified radioresistance-related genes (TOPO2A, PLXDC2, ETNK2, GFI1, and IL12B) were significantly altered in the radioresistant laryngeal cancer cells...
2015: Cancer Biology & Therapy
Jiang Hu, Jun Pan, Zhiguo Luo, Zezhang Tao
Radiotherapy is an important treatment modality for laryngeal carcinoma, however, some patients with early stage laryngeal carcinomas showed resistance to radiotherapy. Survivin overexpression is associated with radioresistance of laryngeal squamous cell carcinoma. We speculated that the inhibition of survivin expression might improve the radiosensitivity of laryngeal carcinoma. We assessed the effect of survivin expressions by shRNA on the radiosensitivity of laryngeal squamous cell carcinoma in vitro and in vivo...
May 2015: Cell Biochemistry and Biophysics
Andreas Geretschläger, Beat Bojaxhiu, Alan Dal Pra, Dominic Leiser, Michael Schmücking, Andreas Arnold, Pirus Ghadjar, Daniel M Aebersold
PURPOSE: To assess clinical outcomes and patterns of loco-regional failure (LRF) in relation to clinical target volumes (CTV) in patients with locally advanced hypopharyngeal and laryngeal squamous cell carcinoma (HL-SCC) treated with definitive intensity modulated radiotherapy (IMRT) and concurrent systemic therapy. METHODS: Data from HL-SCC patients treated from 2007 to 2010 were retrospectively evaluated. Primary endpoint was loco-regional control (LRC). Secondary endpoints included local (LC) and regional (RC) controls, distant metastasis free survival (DMFS), laryngectomy free survival (LFS), overall survival (OS), and acute and late toxicities...
2015: Radiation Oncology
Federico Maria Gioacchini, Matteo Alicandri-Ciufelli, Corrado Rubini, Giuseppe Magliulo, Massimo Re
The aim of this systematic review was to determine the prognostic value of Bcl-2 immunostaining in patients affected by laryngeal squamous cell carcinoma. An appropriate search was conducted on PubMed to retrieve articles dealing with this topic. A double cross-check was performed on citations and full-text articles by 2 investigators independently to review all manuscripts and perform a comprehensive quality assessment. Of 115 abstracts identified, 15 articles were included. These studies reported on 1,150 patients with histologically confirmed diagnosis of laryngeal squamous cell carcinoma...
April 2015: International Journal of Biological Markers
Yunzhen Luo, Yangyang Bao, Shuihong Zhou
No abstract text is available yet for this article.
June 2014: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Sen-Xiang Yan, Xing-Mei Luo, Shui-Hong Zhou, Yang-Yang Bao, Jun Fan, Zhong-Jie Lu, Xin-Biao Liao, Ya-Ping Huang, Ting-Ting Wu, Qin-Ying Wang
PURPOSE: Laryngeal carcinomas always resist to radiotherapy. Hypoxia is an important factor in radioresistance of laryngeal carcinoma. Glucose transporter-1 (GLUT-1) is considered to be a possible intrinsic marker of hypoxia in malignant tumors. We speculated that the inhibition of GLUT-1 expression might improve the radiosensitivity of laryngeal carcinoma. METHODS: We assessed the effect of GLUT-1 expression on radioresistance of laryngeal carcinoma and the effect of GLUT-1 expressions by antisense oligodeoxynucleotides (AS-ODNs) on the radiosensitivity of laryngeal carcinoma in vitro and in vivo...
2013: International Journal of Medical Sciences
Monique M Nijkamp, Paul N Span, Christiaan H J Terhaard, Patricia A H Doornaert, Johannes A Langendijk, Piet L A van den Ende, Martin de Jong, Albert J van der Kogel, Johan Bussink, Johannes H A M Kaanders
Accelerated radiotherapy (AR) improves the poor prognosis associated with epidermal growth factor receptor (EGFR) overexpression frequently seen in head and neck carcinomas. Combining AR with carbogen and nicotinamide (ARCON) counteracts enhanced tumour cell proliferation- and hypoxia-related radioresistance. The purpose of this study was to investigate if EGFR expression levels are associated with response to ARCON in patients with carcinoma of the larynx. Patients (N=272) with advanced stage larynx carcinoma were randomised between AR alone and ARCON...
October 2013: European Journal of Cancer
Wenbo Wang, Lei Yang, Liu Hu, Fen Li, Li Ren, Haijun Yu, Yu Liu, Ling Xia, Han Lei, Zhengkai Liao, Fuxiang Zhou, Conghua Xie, Yunfeng Zhou
The known functions of telomerase in tumor cells include replenishing telomeric DNA and maintaining cell immortality. We have previously shown the existence of a negative correlation between human telomerase reverse transcriptase (hTERT) and radiosensitivity in tumor cells. Here we set out to elucidate the molecular mechanisms underlying regulation by telomerase of radiosensitivity in MCF-7 cells. Toward this aim, yeast two-hybrid (Y2H) screening of a human laryngeal squamous cell carcinoma radioresistant (Hep2R) cDNA library was first performed to search for potential hTERT interacting proteins...
2013: PloS One
Maoxin Wang, Xiaoming Li, Yongtao Qu, Ou Xu, Qingjia Sun
Hypoxia promotes the radioresistance of laryngeal carcinomas and CD133 is one of the markers expressed by tumor-initiating, human laryngeal carcinoma cells. In order to investigate whether CD133-positive Hep-2 cells exhibit a radioresistant phenotype and to determine whether hypoxia promotes this phenotype, we performed a series of experiments. Hep-2 cells, and Hep-2 cells stably expressing hypoxia-inducible factor (HIF)-targeted small interfering RNA (siRNA) were cultured under hypoxic and normoxic conditions and were treated with varying doses of γ-rays (0, 5, 10, 15 and 20 Gy)...
July 2013: International Journal of Oncology
Qing Ke, Junbo Wu, Bangchun Ming, Shengming Zhu, Minghua Yu, Yun Wang, Chenghao Hu, Shuangbing Xu, Zhiguo Luo
Laryngeal carcinoma, as a malignant tumor that occurs in the head and neck region, is widely treated by radiation, but in some cases, the cancer is radioresistant to the radiotherapy. The reason for the radioresistant response needs to be clinically understood. We designed our present study to identify the molecules that may be involved in this radioresistant response. In this study, we initially established the inherent radioresistant (Hep-2max) and radiosensitive (Hep-2min) cell lines from the parental laryngeal cancer cell line Hep-2...
December 2012: Cancer Biotherapy & Radiopharmaceuticals
Anthony C Nichols, Fiona Whelan, John Basmaji, Sandeep Dhaliwal, Samuel Dowthwaite, Corina Chapeskie, Nancy Read, David A Palma, Kevin Fung, Varagur Venkatesan, J Alex Hammond, Jason H Franklin, Iram Siddiqui, Bret Wehrli, Keith Kwan, James Koropatnick, Joe S Mymryk, John W Barrett, John Yoo
BACKGROUND: Early-stage laryngeal squamous cell carcinoma is managed with radiotherapy or endoscopic surgery. Although cure rates are high, radiation failures often require total laryngectomy for salvage. Biomarkers that can predict tumour radioresistance may be useful in modifying the treatment approach for individual patients. METHODS: Retrospective patient chart review yielded 75 patients with T1-T2 glottic squamous cell carcinoma treated with radiation therapy at the London Health Sciences Centre...
April 2012: Journal of Otolaryngology—Head & Neck Surgery
Maoxin Wang, Xiaoming Li, Xiuying Lu, Yongtao Qu, Ou Xu, Qingjia Sun
OBJECTIVE: To study whether cancer stem cells promotes resistance of laryngeal squamous cancer to irradiation mediated by hypoxia. METHOD: Hep-2 cells were respectively cultured in hypoxia and normoxia environment, and the express of HIF-la was detected by western blot. Then they were radiated with different doses of gamma-rays. After that we detected growth inhibition ratio with MTT assay, cell circle and ratio of CD133+ cells with Flow cytometry at different times...
September 2011: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
Kenji Yoshida, Ryohei Sasaki, Hideki Nishimura, Yoshiaki Okamoto, Yoko Suzuki, Tetsuya Kawabe, Miki Saito, Naoki Otsuki, Yoshitake Hayashi, Toshinori Soejima, Kenichi Nibu, Kazuro Sugimura
BACKGROUND: The aim of this study was to evaluate the significance of nuclear factor-kappa B (NF-kappaB) expression as a marker of radioresistance in early-stage laryngeal cancer. METHODS: Thirty-five patients with local recurrence and 70 case-matched patients without local recurrence were entered in this study. NF-kappaB expression was compared with Bcl-2 and epidermal growth factor (EGF) receptor expression by immunohistochemistry, using pretreatment biopsy specimens...
May 2010: Head & Neck
So Young Moon, Hyo Won Chang, Jong-Lyel Roh, Gui Chull Kim, Seung-Ho Choi, Sang-wook Lee, Kyung-Ja Cho, Soon Yuhl Nam, Sang Yoon Kim
Targeting hypoxia-inducible factor-1 (HIF-1) active cells in tumors may be an excellent strategy to improve the outcome of radiation therapy. On the basis of the reported role of YC-1 as a HIF-1 inhibitor with anti-cancer activity, we tested the therapeutic efficacy of YC-1 against radioresistance in vitro. The AMC-HN3 cancer cell line, developed from squamous cell carcinoma of the larynx, was cultured under hypoxic conditions or in the presence of cobalt chloride. Both treatments induced nuclear accumulation of HIF-1alpha protein...
October 2009: Oral Oncology
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