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Alexander M Kiel, Adam G Goodwill, Jillian N Noblet, April L Barnard, Daniel J Sassoon, Johnathan D Tune
This study was designed to identify mechanisms responsible for coronary vasodilation in response to progressive decreases in hematocrit. Isovolemic hemodilution was produced in open-chest, anesthetized swine via concurrent removal of 500 ml of arterial blood and the addition of 500 ml of 37 °C saline or synthetic plasma expander (Hespan, 6% hetastarch in 0.9% sodium chloride). Progressive hemodilution with Hespan resulted in an increase in coronary flow from 0.39 ± 0.05 to 1.63 ± 0.16 ml/min/g (P < 0...
September 30, 2017: Basic Research in Cardiology
Gang Zhao, Wei Wu, Qi-Ming Feng, Jian Sun
OBJECTIVE: The objective of the present study was to explore the resuscitative effect of small-volume resuscitation on uncontrolled hemorrhagic shock in emergency. METHODS: In this study, the resuscitative effects in 200 trauma patients with uncontrolled hemorrhagic shock in emergency were studied. Half of these patients were infused with hypertonic/hyperoncotic fluid (small-volume resuscitation group, n=100), whereas the rest were infused with Hespan and lactated Ringer's solution (conventional fluid resuscitation group, n=100)...
2017: Therapeutics and Clinical Risk Management
Katharina Dullinger, Irene Pamler, Andreas Brosig, Morad Mohrez, Viola Hähnel, Robert Offner, Frauke Dormann, Christine Becke, Ernst Holler, Norbert Ahrens
BACKGROUND: Granulocyte apheresis requires a sedimentation agent. Usually, hydroxyethyl starch (HES) is administered to donors for this purpose and, as granulocyte concentrate (GC) ingredient, also to patients. Authorities recently recommended suspending market authorizations for starch-containing products due to side effects. Therefore, we tested the efficacy of modified fluid gelatin (MFG, Gelafundin 4%) versus hetastarch (Hespan) for GC apheresis. STUDY DESIGN AND METHODS: This retrospective matched-pair analysis compared MFG- and hetastarch-derived GCs...
February 2017: Transfusion
Michelle Griego-Valles, Yekaterina Buriko, Jennifer E Prittie, Philip R Fox
OBJECTIVE: To assess primary and secondary hemostasis following in vitro dilution of canine whole blood (WB) with hydroxyethyl starch (HES) 130/0.4 and HES 670/0.75. DESIGN: In vitro experimental study. SETTING: Private practice, teaching hospital. ANIMALS: Twenty-five healthy dogs. INTERVENTIONS: Each dog underwent venipuncture and 18 mL of venous blood was sampled once. MEASUREMENTS AND MAIN RESULTS: Collected blood was separated in 4 aliquots...
January 2017: Journal of Veterinary Emergency and Critical Care
Marc Cloutier, Carl Simard, Christine Jobin, Diane Fournier, Sonia Néron
BACKGROUND: Preparation of umbilical cord blood units (CBUs) for infusion requires a step of dilution or washing to reduce the toxicity of the dimethyl sulfoxide present in the freezing solution. However, the worldwide shortage of clinical-grade dextran 40, the most widely used cord blood dilution or washing solution, prompted us to search for an alternative solution. We elected to evaluate the performance of alternative solutions that could be used as potential replacements for the dextran 40-based solution...
July 2016: Transfusion
Nimit Patel, Olga Solovyova, Greg Matthews, Sivasenthil Arumugam, Sanjay K Sinha, Courtland G Lewis
In a retrospective analysis, we evaluated the safety and efficacy of peripheral nerve blocks (PNB) compared to epidural anesthesia in 221 consecutive patients undergoing same-day bilateral total knee arthroplasty (TKA). Primary outcome measures included: hypotension requiring physician intervention, number of blood transfusions, perioperative hespan and crystalloid consumption, incidences of respiratory desaturation, pruritis, urinary retention, and nausea/vomiting. The incidences of hypotension, urinary retention, and pruritis were all higher in the epidural group, compared to PNB...
February 2015: Journal of Arthroplasty
Yu-Jen Chang, Kuei-Erh Tien, Cheng-Hao Wen, Tzu-Bou Hsieh, Shiaw-Min Hwang
BACKGROUND AIMS: Very small embryonic-like (VSEL) stem cells are a rare cell population present in bone marrow, cord blood and other tissues that displays a distinct small cell size and the ability to give rise to cells of the three germ layers. VSEL stem cells were reported to be discarded in the red blood cell fraction by Ficoll-Paque density gradient centrifugation during the processing of bone marrow and cord blood specimens. However, most cord blood banks do not include density gradient centrifugation in their procedures while red blood cells are removed by Hespan sedimentation following the Cord Blood Transplantation Study cord blood bank standard operating procedures (COBLT SOP)...
April 2014: Cytotherapy
J N Mehrishi, Tibor Bakács
BACKGROUND: Umbilical cord blood (UCB) is rich in the heavily glycosylated CD34 antigen-bearing hematopoietic stem cells that are valuable for transplantation therapy of malignant and nonmalignant disease. CD34+ cell yields (0.13%-0.25%-0.3%) of mononuclear cells (UCMCs) isolated by anti-CD34 monoclonal antibody (MoAb) on immunomagnetic particles (e.g., Miltenyi particles) are insufficient to treat adults. STUDY DESIGN AND METHODS: We fractionated UCMCs by physicochemical charge-based methods...
November 2013: Transfusion
J N Mehrishi
Umbilical cord blood (UCB) hematopoietic stem cells (HSC-CD34+) are valuable for treating malignant or nonmalignant disease. Processing UCB by HESPAN-6% and anti-CD34-Miltenyi particles provides insufficient cells for treating adults. Physicochemical-electrokinetic studies on UCB-mononuclear cells (MNCs) under conditions of delayed processing, ice or very low temperatures, and some cell separation media identified artifacts introduced by procedures. Adsorption of biomaterials from cell damage by temperature, degradation products after using enzymes, harsh reagents, dithiothreitol, and HESPAN affect cell properties and distribution...
November 2013: Transfusion
Daniel N Darlington, Angel V Delgado, Bijan S Kheirabadi, Chriselda G Fedyk, Michael R Scherer, Anthony E Pusateri, Charles E Wade, Andrew P Cap, John B Holcomb, Michael A Dubick
BACKGROUND: This study evaluates the effect of hemodilution by various common resuscitation fluids, and the efficacy of activated recombinant factor VII (rFVIIa) on coagulation parameters in human blood in vitro. METHODS: Samples from normal healthy volunteers (n = 9) were hemodiluted from 0% to 90% with normal saline, or 0%, 40%, 60%, and 80% with 5% albumin, Hespan, Hextend, normal saline, or lactated Ringer's, and incubated at 37°C ± 1°C for 30 minutes with and without rFVIIa (1...
November 2011: Journal of Trauma
Ahmed Turkistani, Khalid Abdullah, Essam Manaa, Bilal Delvi, Gamal Khairy, Badiah Abdulghani, Nancy Khalil, Fatma Damas, Abdelazeem El-Dawlatly
BACKGROUND: Postoperative nausea and vomiting (PONV) is a common complication following general anesthesia. Different regimens have been described for the treatment of PONV with few that mention the prevention of it. Therefore, we conducted this study to compare the effect of preloading with either crystalloids or colloids on the incidence of PONV following laparoscopic cholecystectomy (LC), under general anesthesia. MATERIALS AND METHODS: This study was carried out on 80 patients who underwent LC...
July 2009: Saudi Journal of Anaesthesia
Min Li, Shu-Ying Fan, Guang-Hui Sun, Pei-Ru Xu, Aerziguli Turerxun
OBJECTIVE: To compare the characteristics of immunomagnetic beads and hespan precipitation for isolation of mononuclear cells (MNCs) from umbilical cord blood and try to find a better isolation method for MNCs. METHODS: Fifteen umbilical cord blood samples from healthy parturiens were collected between December 2007 and March 2008. MNCs were isolated using hespan precipitation and CD133 immunomagnetic beads, respectively. MNCs were identified using the surface marker CD34 by flow cytometry on the 30th of primary culture...
September 2009: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
Hasan B Alam, Christian Shults, Naresh Ahuja, Eduardo C Ayuste, Huazhen Chen, Elena Koustova, Elizabeth A Sailhamer, Yongqing Li, Baoling Liu, Marc de Moya, George C Velmahos
BACKGROUND: Chromatin remodeling through histone acetylation is a key control mechanism in gene transcription. We have shown previously that fluid resuscitation in rodents is coupled with highly structured post-translational modifications of cardiac histones. The current experiment was performed to validate this concept in a clinically relevant large animal model of hemorrhage and resuscitation, and to correlate the changes in histone acetylation with altered expression of immediate-early response genes...
February 2008: Resuscitation
Anthony T Cheung, Patricia L Duong, Bernd Driessen, Peter C Chen, Jonathan S Jahr, Robert A Gunther
Systemic function and oxygenation changes during hemorrhagic shock treatment were continuously monitored and correlated with real-time microvascular changes. After splenectomy, each dog (n = 12) was hemorrhaged (MAP = approximately 50 mmHg; approximately 40% blood loss = 32-36 ml/kg) and randomly assigned to 4 resuscitation groups: autologous/shed blood, hemoglobin-based oxygen-carrier/Oxyglobin, crystalloid/saline, and colloid/Hespan. Systemic function and oxygenation changes were continuously monitored and measured using standard operating room protocols...
2006: Clinical Hemorheology and Microcirculation
Eduardo C Ayuste, Huazhen Chen, Elena Koustova, Peter Rhee, Naresh Ahuja, Zhang Chen, C Robert Valeri, Konstantinos Spaniolas, Tina Mehrani, Hasan B Alam
BACKGROUND: Cytotoxic properties of racemic (D-,L-isomers) lactated Ringer's solution detected in vitro and in small animal experiments, have not been confirmed in large animal models. Our hypothesis was that in a clinically relevant large animal model of hemorrhage, resuscitation with racemic lactated Ringer's solution would induce cellular apoptosis, which can be attenuated by elimination of d-lactate. METHODS: Yorkshire swine (n = 49, weight 40-58 kg) were subjected to uncontrolled (iliac arterial and venous injuries) and controlled hemorrhage, totaling 40% of estimated blood volume...
January 2006: Journal of Trauma
Angie L Vlahos, Robert S Crawford, Howard T Matthew, Anna M Ledgerwood, Charles E Lucas
BACKGROUND: Sepsis and hemorrhagic shock (HS) cause hypoalbuminemia, with extravascular albumin relocation. Prior studies on cultured rat hepatocytes showed a fall in albumin genesis when exposed to albumin and Hespan (HES) supplementation. This study assesses the effects of increased colloid osmotic pressure on albumin and urea genesis from hepatocytes exposed to HS or septic insult before harvesting. METHODS: Hepatocyte synthesis of albumin was measured in an in vitro collagen sandwich preparation with a seeding density of 1 million cells per dish in Dulbecco's modified Eagle's medium without additional colloid (control cultures)...
September 2005: Journal of Trauma
Timothy Gannon, Kristen McConnell, Jeffrey Riley
The use of hydroxy-ethyl starch (HES) has been used in the cord blood banking industry for the separation of blood into its individual components. The focus of this study is to examine whether the use of HES is feasible in obtaining a buffy coat for the use in the construction of platelet gels. Blood was collected from seven canines into anticoagulant citrate dextrose at a ratio of 1:8. A sample of whole blood was used to obtain initial cell counts and a base line Thrombelastograph (TEG). 6% Hespan was added to the whole blood and allowed to sediment for 60 minutes...
September 2005: Journal of Extra-corporeal Technology
Stephen E Dailey, Christopher B Dysart, Daniel R Langan, Michael J Slye, Gregory A Nuttall, Lisa M Schrader, Brent A Williams, William C Oliver
OBJECTIVE: The purpose of this study was to determine if 6% HES 450/0.7 (hydroxyethyl starch 450/0.7) in normal saline (Hespan) and 6% HES 450/0.7 in lactated Ringer's solution (Hextend) have the same inhibition of the intrinsic coagulation pathway and platelet function. Multiple studies have suggested that 6% Hespan inhibits coagulation and increases chest tube drainage and transfusion requirements in cardiac surgical patients. There have been few studies of the effects of 6% Hextend, a relatively new plasma volume expander, on coagulation and the results thus far have been mixed...
June 2005: Journal of Cardiothoracic and Vascular Anesthesia
Anthony T W Cheung, Bernd Driessen, Jonathan S Jahr, Patricia L Duong, Sahana Ramanujam, Peter C Y Chen, Robert A Gunther
Blood substitute resuscitation as a treatment modality for moderate hypovolemia (approximately 40% blood loss) in a canine model has been evaluated using Oxyglobin (Biopure Hemoglobin Glutamer-200/ Bovine; a hemoglobin-based oxygen-carrier) and Hespan (6% hetastarch; a nonoxygen-carrier) as resuscitants. Autologous (shed) blood served as control. Nine dogs were studied--after splenectomy, each dog was hemorrhaged (32-36 mL/kg; MAP = approximately 50 mmHg) and randomly assigned to the three resuscitation groups...
May 2004: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Eugene Vovenko, Aleksander Golub, Roland Pittman
No abstract text is available yet for this article.
2003: Advances in Experimental Medicine and Biology
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