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wheat bran

Liangkun Long, Haoyuan Zhao, Dafan Ding, Meijuan Xu, Shaojun Ding
Feruloyl esterase (FAE)-encoding genes AnfaeA and AnfaeB were isolated from Aspergillus niger 0913. For overexpression of the two genes in Trichoderma reesei, constitutive and inductive expression plasmids were constructed based on parental plasmid pAg1-H3. The constructed plasmids contained AnfaeA or AnfaeB gene under the control of glyceraldehyde-3-phosphate dehydrogenase A gene (gpdA) promoter (from A. nidulans) or cellobiohydrolases I (cbh I) gene promoter (from T. reesei), and cbh I terminator from T. reesei...
January 18, 2018: Bioprocess and Biosystems Engineering
Amina Elahi, Abdul Rehman
In the present work, a yeast strain Pichia kudriavzevii was identified on the basis of 18S rDNA, showing maximum growth at 30°C and pH 7.0. Among all the complex polysaccharides used, wheat bran proved to be the best substrate as indicated by the maximum growth of the yeast strain. The yeast isolate was capable of producing xylanase both intra- and extra-cellularly, the dominant form being extracellular. The maximum enzyme activity was determined at pH 5.0 and at 50°C. Na+, Mg2+ and Fe2+ presence caused a substantial increase in enzyme activity while a slight decrease (4...
January 11, 2018: Revista Argentina de Microbiología
Lin Liu, Weili Gong, Xiaomeng Sun, Guanjun Chen, Lushan Wang
Byproducts of food processing can be utilized for the production of high-value-added enzyme cocktails. In this study, we utilized integrated functional-omics technology to analyze composition and functional characteristics of extracellular enzymes produced by Aspergillus niger grown on food processing byproducts. The results showed that oligosaccharides constituted by arabinose, xylose, and glucose in wheat bran were able to efficiently induce the production of extracellular enzymes of A. niger. Compared with other substrates, wheat bran was more effective at inducing the secretion of β-glucosidases from GH1 and GH3 families, as well as >50% of proteases from A1-family aspartic proteases...
January 15, 2018: Journal of Agricultural and Food Chemistry
D Ruan, A M Fouad, Q L Fan, W Chen, W G Xia, S Wang, Y Y Cui, Y Wang, L Yang, C T Zheng
The study investigated the effects of increasing content of corn distillers' dried grains with solubles (DDGS) in the diets of laying ducks on oxidative status, laying performance, egg quality, and egg yolk fatty acid composition. Longyan females (1080) with similar BW at 17 wk of age were randomly assigned to 6 treatment groups, each consisting of 6 replicates of 30 birds. The basal diet (I) was a typical corn-soybean ration while the experimental diets (II to VI) substituted corn DDGS for soybean meal and wheat bran and a small reduction of corn...
November 29, 2017: Poultry Science
Yu-Si Yan, Shuai Zhao, Lu-Sheng Liao, Qi-Peng He, Ya-Ru Xiong, Long Wang, Cheng-Xi Li, Jia-Xun Feng
Background: The transition to a more environmentally friendly economy has prompted studies of modern biorefineries, including the utilization of low-value lignocellulose. The major challenge facing the widespread application of biorefineries is the high cost of enzymes that can efficiently hydrolyze recalcitrant cellulose to sugars. Penicillium oxalicum produces large amounts of plant-cell-wall-degrading enzymes, but their production is tightly controlled by complex regulatory networks, resulting in low yields of the native enzymes...
2017: Biotechnology for Biofuels
Miriam Zanoletti, Alessandra Marti, Mauro Marengo, Stefania Iametti, M Ambrogina Pagani, Stefano Renzetti
A molecular and material science approach is used to describe the influence of coarse and fine buckwheat bran on wheat dough properties and bread textural quality. Focus is given on (i) gluten solvation and structural arrangements in presence of bran as studied by front-face fluorescence; (ii) thermo-mechanical behavior of dough during heating studied by dynamic mechanical thermal analysis and (iii) texture of bread crumb analyzed in terms of a cellular solid. The thermo-mechanical behavior of dough was found to be largely related to starch phase transitions during heating...
December 2017: Food Research International
Qian Li, Rui Liu, Tao Wu, Min Zhang
The present study assesses the aggregation behavior of wheat bran arabinoxylan-rich soluble dietary fiber (SDF) fractions with diverse molecular weight and substitution in order to provide useful information to prevent the formation of a block network. In the present work, dynamic and static light scattering, diffusing wave spectroscopy, small amplitude dynamic rheology, atomic force microscopy, and the water-holding and swelling capacities were evaluated to assess the SDF aggregation behavior induced by intrinsic and extrinsic factors...
December 2017: Food Research International
Preeti Tripathi, Jyothi Lakshmi A, Mukesh Kapoor
The present study was aimed at enhancing phytase (Phy-Ck) production from Citrobacter koseri PM-7 using response surface methodology (RSM) and improving the bioaccessibility of minerals (Fe and Zn) and protein digestibility in high-phytate food using Phy-Ck. A five-variable and three-level central composite design of RSM using wheat bran (6.681% w/v), inoculum level (2.5% v/v), and triton-x-100 (0.2% v/v) resulted in up to 5.57-fold (1.047 U/ml) improvement in Phy-Ck yield from C. koseri PM-7 when compared with fermentation media I & II...
December 1, 2017: Preparative Biochemistry & Biotechnology
João Heitor C Manfrão-Netto, Thiago M Mello-de-Sousa, Astrid R Mach-Aigner, Robert L Mach, Marcio J Poças-Fonseca
Humicola grisea var. thermoidea (Hgvt) is a thermophilic ascomycete that produces lignocellulolytic enzymes and it is proposed for the conversion of agricultural residues into useful byproducts. Drugs that inhibit the DNA methyltransferases (DNMTs) activity are employed in epigenetic studies but nothing is known about a possible effect on the production of fungal enzymes. We evaluated the effect of 5-aza-2'-deoxycytidine (5-Aza; a chemical inhibitor of DNMTs activity) on the secreted enzyme activity and on the transcription of cellulase and xylanase genes from Hgvt grown in agricultural residues and in glucose...
November 28, 2017: Journal of Basic Microbiology
Brahim Bchir, Thiry Jean-François, Holy Nadia Rabetafika, Christophe Blecker
Cereal bars are nutritious food composed of several ingredients including dry raw and agglutinative ingredients. The objective of this study was to evaluate the effect of pear apple and date fibres, from cooked fruit co-product, addition on the physico-chemical textural and sensory properties of cereal bars. First, five formulations containing an amount of 10% of co-products and commercial fibre, used as a reference, were elaborated and their physico-chemical composition was determined. Second, to determine the acceptability of consumer, apple fibre co-products were added (6, 10 and 14%)...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
Silvia Hüttner, Thanh Thuy Nguyen, Zoraide Granchi, Thomas Chin-A-Woeng, Dag Ahrén, Johan Larsbrink, Vu Nguyen Thanh, Lisbeth Olsson
Background: Genome and transcriptome sequencing has greatly facilitated the understanding of biomass-degrading mechanisms in a number of fungal species. The information obtained enables the investigation and discovery of genes encoding proteins involved in plant cell wall degradation, which are crucial for saccharification of lignocellulosic biomass in second-generation biorefinery applications. The thermophilic fungus Malbranchea cinnamomea is an efficient producer of many industrially relevant enzymes and a detailed analysis of its genomic content will considerably enhance our understanding of its lignocellulolytic system and promote the discovery of novel proteins...
2017: Biotechnology for Biofuels
Renganath Rao Ramesh, Vimudha Muralidharan, Saravanan Palanivel
Usage of the animal fleshing waste as the source of carbon and nitrogen for animal skin unhairing protease (EC 3.4.21) production along with agro-industrial wastes like wheat bran has been investigated. Thermal hydrolysis of delimed fleshing waste for 3 h yielded a fleshing hydrolysate (FH) having a protein content of 20.86 mg/mL and total solids of 46,600 ppm. The FH was lyophilized and spray dried to obtain fleshing hydrolysate powder (FHP) to be used along with wheat bran and rice bran for protease production...
November 6, 2017: Environmental Science and Pollution Research International
Minmin Cai, Ruiqi Hu, Ke Zhang, Shiteng Ma, Longyu Zheng, Ziniu Yu, Jibin Zhang
Treating municipal sewage sludge (MSS) sustainably and economically in China remains a challenge because of risks associated with the heavy metals it contains. In this study, black solider fly larvae (BSFL) were used for MSS treatment. The resistance of larvae to combined heavy metals and their potential use in conversion of MSS were investigated. The results indicated that seven MSS samples contained large amounts of heavy metals, with the lead and nickel contents of several samples exceeding Chinese national discharge standards...
November 2, 2017: Environmental Science and Pollution Research International
Arpita Vats, Saroj Mishra
Multiplicity in laccases among lignin degrading fungal species is of interest as it confers the ability to degrade several types of lignocellulosics. The combination of laccases produced on such substrates could be beneficial for treatment of complex aromatics, including dyes. In this study, we report on production of high units (679.6Ug(-1) substrate) of laccase on solid wheat bran (WB) by Cyathus bulleri. Laccase, purified from the culture filtrates of WB grown fungus, was effective for oxidation of veratryl alcohol, Reactive blue 21 and textile effluent without assistance of externally added mediators...
October 23, 2017: Journal of Hazardous Materials
Diogo Taschetto, Sergio Luiz Vieira, Clara Roselina Angel, Catarina Stefanello, Liris Kindlein, Marco Antonio Ebbing, Cristina Tonial Simões
A study was conducted to investigate Fe requirements of broiler breeders. One-hundred-fifty-six Cobb 500 broiler breeder hens were individually placed in electrostatically painted cages at 22 weeks. The study was composed of an adaptation phase, in which hens were fed corn-soy-wheat bran diets until 35 wks. An Fe deficient mash diet (24.6 ppm Fe) was provided from 35 to 46 wk in order to induce a partial body Fe depletion. A production phase followed from 47 to 70 wk when hens were fed 6 diets with increasing Fe sulfate supplementation, which, upon analyses had 24...
September 1, 2017: Poultry Science
Sharanappa T Talawar, Nanishankar V Harohally, Chetana Ramakrishna, G Suresh Kumar
Wheat bran, an abundant byproduct of the milling industry, comprises fat-soluble bioactives and fibers. In the present study, two concentrates were prepared from wheat bran oil (WBO) using silicic acid coupled with acetone (WBA) and hexane (WBH). WBA extract had enhanced color and viscosity and was enriched with fat-soluble bioactives (sterols, oryzanol-like compounds, tocopherols, and carotenoids) as evidenced from NMR and other techniques. In in vitro studies, WBA exhibited significant free radical scavenging activity, limited DNA and LDL oxidation, and inhibiting HMG-CoA reductase and lipase activity better than WBH and WBO...
October 31, 2017: Journal of Agricultural and Food Chemistry
Arkadiusz Damian Liśkiewicz, Daniela Kasprowska-Liśkiewicz, Anna Sługocka, Marta Maria Nowacka-Chmielewska, Jan Wiaderkiewicz, Halina Jędrzejowska-Szypułka, Jarosław Jerzy Barski, Joanna Lewin-Kowalik
The high fat and low carbohydrate ketogenic diet (HFKD) is extensively studied within the fields of numerous diseases, including cancer and neurological disorders. Since most studies incorporate animal models, ensuring the quality of ketogenic rodent diets is important, both in the context of laboratory animal welfare as well as for the accuracy of the obtained results. In this study we implemented a modification to a commonly-used ketogenic rodent chow by replacing non-resorbable cellulose with wheat bran...
October 18, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
Bin Xiao Fu, Constance Chiremba, Curtis J Pozniak, Kun Wang, Shin Nam
The aim of this study was to investigate the distribution of total yellow pigments, total phenolic compounds, and their antioxidant activities in various durum wheat milling fractions. Carotenoid composition of yellow pigment extract was also examined using UPLC. The ABTS radical scavenging activity of the milling fractions decreased in the order of short bran/bran > feed flour > flour/semolina in both total phenolic and total yellow pigment extracts. Yellow pigments extracts from bran, short bran, and feed flour exhibited 5...
October 14, 2017: Antioxidants (Basel, Switzerland)
Joanna E Kowalczyk, Claire Khosravi, Samuel Purvine, Alice Dohnalkova, William B Chrisler, Galya Orr, Errol Robinson, Erika Zink, Ad Wiebenga, Mao Peng, Evy Battaglia, Scott Baker, Ronald P de Vries
In A. niger, two transcription factors, AraR and XlnR, regulate the production of enzymes involved in degradation of arabinoxylan and catabolism of the released l-arabinose and d-xylose. Deletion of both araR and xlnR in leads to reduced production of (hemi)cellulolytic enzymes and reduced growth on arabinan, arabinogalactan and xylan. In this study, we investigated the colonization and degradation of wheat bran by the A. niger reference strain CBS 137562 and araR/xlnR regulatory mutants using high-resolution microscopy and exo-proteomics...
October 13, 2017: Environmental Microbiology
A C B Menezes, S C Valadares Filho, P P Rotta, S A Santos, M V C Pacheco, B C Silva, P Pucetti, H M Alhadas, E Detmann, J S Caton
The effects of microbial contamination (MC) on CP degradability of concentrate feeds are still controversial. Therefore, the aim of this study was to use N to estimate the impact of MC on estimations of CP fractions (the soluble fraction of CP [a], the insoluble but potentially degradable fraction of CP [b], and the rate of digestion of fraction b [kd]) of concentrate feeds. Twelve types of feed were evaluated: 6 energy concentrates-wheat bran ( L.), rice meal ( L.), ground corn ( L.), ground sorghum ( Pers...
September 2017: Journal of Animal Science
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