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wheat bran

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https://www.readbyqxmd.com/read/27927976/metabolic-profiling-reveals-differences-in-plasma-concentrations-of-arabinose-and-xylose-after-consumption-of-fiber-rich-pasta-and-wheat-bread-with-differential-rates-of-systemic-appearance-of-exogenous-glucose-in-healthy-men
#1
Andre J Pantophlet, Suzan Wopereis, Coby Eelderink, Roel J Vonk, Johanna H Stroeve, Sabina Bijlsma, Leo van Stee, Ivana Bobeldijk, Marion G Priebe
BACKGROUND: The consumption of products rich in cereal fiber and with a low glycemic index is implicated in a lower risk of metabolic diseases. Previously, we showed that the consumption of fiber-rich pasta compared with bread resulted in a lower rate of appearance of exogenous glucose and a lower glucose clearance rate quantified with a dual-isotope technique, which was in accordance with a lower insulin and glucose-dependent insulinotropic polypeptide response. OBJECTIVE: To gain more insight into the acute metabolic consequences of the consumption of products resulting in differential glucose kinetics, postprandial metabolic profiles were determined...
December 7, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27924363/characteristics-and-applications-of-recombinant-thermostable-amylopullulanase-of-geobacillus-thermoleovorans-secreted-by-pichia-pastoris
#2
M Nisha, T Satyanarayana
The 3'-deleted amylopullulanase gene from the extreme thermophile Geobacillus thermoleovorans (Gt-apuΔC) was expressed extracellularly in Pichia pastoris under both methanol-inducible AOX1 and constitutive GAP promoters. The expression of the gene (Gt-apuΔC) was higher under GAP promoter (36.2 U ml(-1), α-amylase; 33.5 U ml(-1), pullulanase) than that under AOX1 promoter (32.5 and 28.6 U ml(-1)). The heavily glycosylated Gt-apuΔC from the recombinant P. pastoris displays higher substrate specificity, thermal stability and starch saccharification efficiency than that expressed in Escherichia coli...
December 6, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27896860/preference-of-goats-capra-hircus-l-for-tanniniferous-browse-species-available-in-semi-arid-areas-in-ethiopia
#3
G Mengistu, M Bezabih, W H Hendriks, W F Pellikaan
The objectives were to determine browse species preference of goats using dry matter intake (DMI) as a proxy, to compare preference when offered in combination with polyethylene glycol (PEG) and to establish relationships between browse species intake and chemical compositional data. Air-dried leaves of Acacia etbaica, Cadaba farinosa, Capparis tomentosa, Dichrostachys cinerea, Dodonaea angustifolia, Euclea racemosa, Maerua angolensis, Maytenus senegalensis, Rhus natalensis and Senna singueana were used. Two cafeteria trials, each lasting 10 days, were conducted using four local mature male goats of 2-2...
November 29, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27878454/pectinase-production-by-aspergillus-giganteus-in-solid-state-fermentation-optimization-scale-up-biochemical-characterization-and-its-application-in-olive-oil-extraction
#4
Gastón E Ortiz, María C Ponce-Mora, Diego G Noseda, Gabriela Cazabat, Celina Saravalli, María C López, Guillermo P Gil, Martín Blasco, Edgardo O Albertó
The application of pectinases in industrial olive-oil processes is restricted by its production cost. Consequently, new fungal strains able to produce higher pectinase titers are required. The aim of this work was to study the capability of Aspergillus giganteus NRRL10 to produce pectinolytic enzymes by SSF and evaluate the application of these in olive-oil extraction. A. giganteus was selected among 12 strains on the basis of high pectinolytic activity and stability. A mixture composed by wheat bran, orange, and lemon peels was selected as the best substrate for enzyme production...
November 22, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27876437/the-content-and-distribution-of-steryl-ferulates-in-wheat-produced-in-japan
#5
Wakako Tsuzuki, Hiroyuki Mogushi, Shuji Kawahara, Eiichi Kotake-Nara, Shiro Komba, Yoshikazu Kanai, Sumiyo Yamada, Akira Horigane
Oryzanol contained in rice bran is a complex mixture of steryl ferulates (SFs) with many identified health benefits. Recently, SF has been shown to exist in other cereals such as wheat, rye, and corn. In this study, SFs in several wheats produced in Japan were analyzed. For instance, SF content of whole wheat grain, Yumekaori (Japan) was 15.2 ± 1.4 mg-oryzanol-equivalent/100 g grain, while that of the imported one, 1CW (Canada) was 11.4 ± 1.3 mg-oryzanol-equivalent/100 g grain. The main SF components in the examined wheats were campesteryl ferulate, campestanyl ferulate, and sitostanyl ferulate...
November 23, 2016: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/27872878/serum-antioxidant-capacity-and-peroxide-level-of-seven-healthy-subjects-after-consumption-of-different-foods
#6
Maura N Laus, Mario Soccio, Michela Alfarano, Antonella Pasqualone, Marcello S Lenucci, Giuseppe Di Miceli, Donato Pastore
This article reports experimental data related to the research article entitled "Different effectiveness of two pastas supplemented with either lipophilic or hydrophilic/phenolic antioxidants in affecting serum as evaluated by the novel Antioxidant/Oxidant Balance approach" (M.N. Laus, M. Soccio, M. Alfarano, A. Pasqualone, M.S. Lenucci, G. Di Miceli, D. Pastore, 2016) [1]. Antioxidant status of blood serum of seven healthy subjects was evaluated during four hours after consumption of two functional pastas, supplemented with either bran oleoresin or bran water extract obtained from durum wheat...
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27863994/understanding-the-physics-of-functional-fibers-in-the-gastrointestinal-tract-an-evidence-based-approach-to-resolving-enduring-misconceptions-about-insoluble-and-soluble-fiber
#7
Johnson W McRorie, Nicola M McKeown
Enduring misconceptions about the physical effects of fiber in the gut have led to misunderstandings about the health benefits attributable to insoluble and soluble fiber. This review will focus on isolated functional fibers (eg, fiber supplements) whose effects on clinical outcomes have been readily assessed in well-controlled clinical studies. This review will also focus on three health benefits (cholesterol lowering, improved glycemic control, and normalizing stool form [constipation and diarrhea]) for which reproducible evidence of clinical efficacy has been published...
November 15, 2016: Journal of the Academy of Nutrition and Dietetics
https://www.readbyqxmd.com/read/27844340/production-of-mycophenolic-acid-by-penicillium-brevicompactum-using-solid-state-fermentation
#8
Gopal Patel, Mahesh D Patil, Surbhi Soni, Yusuf Chisti, Uttam Chand Banerjee
Solid-state fermentation using the microfungus Penicillium brevicompactum for the production of mycophenolic acid is reported in this paper. Of the initial substrates tested (whole wheat, cracked wheat, long grain Basmati rice, and short grain Parmal rice), Parmal rice proved to be the best. Under initial conditions, using steamed Parmal rice with 80% (w/w) initial moisture content, a maximum mycophenolic acid concentration of 3.4 g/kg substrate was achieved in 12 days of fermentation at 25 °C. The above substrate was supplemented with the following additional nutrients (g/L packed substrate): glucose 40...
November 14, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27824279/agricultural-wastes-as-substrates-for-%C3%AE-glucosidases-production-by-talaromyces-thermophilus-role-of-these-enzymes-in-enhancing-waste-paper-saccharification
#9
Hanen Mallek-Fakhfakh, Jawhar Fakhfakh, Najla Masmoudi, Fatma Rezgui, Ali Gargouri, Hafedh Belghith
In the present study we investigated a potent extracellular β-glucosidases secreted by the thermophilic fungal strain AX4 of Talaromyces thermophilus, isolated from Tunisian soil samples. This strain was selected referring to the highest thermostability of its β-glucosidases compared to the other fungal isolates. The β-glucosidase production was investigated by submerged fermentation (SmF). The optimal temperature and initial pH for maximum β-glucosidase production were 50°C and 7.0, respectively. Several carbon sources were assayed for their effects on β-glucosidase production, significant yields were obtained in media containing lactose 1% (3...
November 8, 2016: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27798344/obese-mice-fed-a-diet-supplemented-with-enzyme-treated-wheat-bran-display-marked-shifts-in-the-liver-metabolome-concurrent-with-altered-gut-bacteria
#10
Dorothy A Kieffer, Brian D Piccolo, Maria L Marco, Eun Bae Kim, Michael L Goodson, Michael J Keenan, Tamara N Dunn, Knud Erik Bach Knudsen, Sean H Adams, Roy J Martin
BACKGROUND: Enzyme-treated wheat bran (ETWB) contains a fermentable dietary fiber previously shown to decrease liver triglycerides (TGs) and modify the gut microbiome in mice. It is not clear which mechanisms explain how ETWB feeding affects hepatic metabolism, but factors (i.e., xenometabolites) associated with specific microbes may be involved. OBJECTIVE: The objective of this study was to characterize ETWB-driven shifts in the cecal microbiome and to identify correlates between microbial changes and diet-related differences in liver metabolism in diet-induced obese mice that typically display steatosis...
October 19, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27792924/production-of-%C3%AE%C2%B5-poly-lysine-by-streptomyces-albulus-pd-1-via-solid-state-fermentation
#11
Delei Xu, Haiqing Yao, Zhaoxian Xu, Rui Wang, Zheng Xu, Sha Li, Xiaohai Feng, Youhua Liu, Hong Xu
The aim of this study was to produce ε-poly-lysine (ε-PL) by Streptomyces albulus PD-1 through solid-state fermentation (SSF) using agro-industrial residues. Maximum ε-PL production (86.62mg/g substrate) was obtained a mixed substrate of rapeseed cake and wheat bran (2:1, w/w) supplemented with glucose (4%, w/w), (NH4)2SO4 (3%, w/w), with an initial moisture content of 65%, initial pH of 7.0 and inoculum size of 13% v/w, incubated at 30°C for 8days. The results of scanning electron microscopy indicated that the filamentous thallus could penetrate the substrate surface...
January 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27790372/aflatoxin-b1-zearalenone-and-deoxynivalenol-in-feed-ingredients-and-complete-feed-from-different-province-in-china
#12
Li Wu, Jianjun Li, Yunhu Li, Tiejun Li, Qinghua He, Yulong Tang, Hongnan Liu, Yongteng Su, Yulong Yin, Peng Liao
BACKGROUND: The current study was carried out to provide a reference for monitory of aflatoxin B1 (AFB1), zearalenone (ZEN) and deoxynivalenol (DON) contamination in feed ingredients and complete feeds were collected from different Province in China from 2013 to 2015. METHODS: A total of 443 feed ingredients, including 220 corn, 24 wheat, 24 domestic distillers dried grains with soluble (DDGS), 55 bran, 20 wheat shorts and red dog, 37 imported DDGS, 34 corn germ meal and 29 soybean meal as well as 127 complete feeds including 25 pig complete feed (powder), 90 pig complete feed (pellet), six duck complete feed and six cattle complete feed were randomly collected from different Province in China, respectively, by high-performance chromatography in combined with UV or fluorescence analysis...
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/27789882/kinetic-characterization-thermo-stability-and-reactive-red-195a-dye-detoxifying-properties-of-manganese-peroxidase-coupled-gelatin-hydrogel
#13
Muhammad Bilal, Muhammad Asgher, Hongbo Hu, Xuehong Zhang
An indigenous and industrially important manganese peroxidase (MnP) was isolated from solid-state bio-processing of wheat bran by white-rot fungal strain Ganoderma lucidum IBL-05 under pre-optimized growth conditions. Crude MnP extract was partially purified (2.34-fold) to apparent homogeneity by ammonium sulphate precipitation and dialysis. The homogeneous enzyme preparation was encapsulated on gelatin matrix using glutaraldehyde as a cross-linking agent. Optimal conditions for highest immobilization (82.5%) were: gelatin 20% (w/v), glutaraldehyde 0...
October 2016: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/27780957/purification-and-properties-of-extracellular-lipases-with-transesterification-activity-and-1-3-regioselectivity-from-rhizomucor-miehei-and-rhizopus-oryzae
#14
Miklós Takó, Alexandra KotogÁn, TamÁs Papp, Shine Kadaikunnan, Naiyf S Alharbi, Csaba VÁgvölgyi
Rhizomucor miehei NRRL 5282 and Rhizopus oryzae NRRL 1526 can produce lipases with high synthetic activities in wheat bran-based solid state culture. In this study, purification and biochemical characterization of the lipolytic activities of these lipases are presented. SDS-PAGE indicated a molecular mass of about 55 and 35 kDa for the purified R. miehei and Rh. oryzae enzymes, respectively. p-Nitrophenyl palmitate (pNPP) hydrolysis was maximal at 40°C and pH 7.0 for the R. miehei lipase, and at 30°C and pH 5...
October 25, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27770795/replacement-of-carbohydrate-binding-modules-improves-acetyl-xylan-esterase-activity-and-its-synergistic-hydrolysis-of-different-substrates-with-xylanase
#15
Shiping Liu, Shaojun Ding
BACKGROUND: Acetylation of the xylan backbone was a major obstacle to enzymatic decomposition. Removal of acetyl groups by acetyl xylan esterases (AXEs) is essential for completely enzymatic hydrolysis of xylan. Appended carbohydrate binding modules (CBMs) can promote the enzymatic deconstruction of plant cell walls by targeting and proximity effects. Fungal acetyl xylan esterases are strictly appended to cellulose-specific CBM1. It is still unclear whether xylan-specific CBMs have a greater advantage than CBM1 in potentiating the activity of fungal deacetylating enzymes and its synergistic hydrolysis of different substrates with xylanase...
October 22, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27765973/enhancing-antioxidant-activity-and-antiproliferation-of-wheat-bran-through-steam-flash-explosion
#16
Yongsheng Chen, Ruiting Zhang, Chong Liu, Xueling Zheng, Benguo Liu
The effect of steam flash explosion (SFE), a green processing technology, on the phenolic composition, antioxidant activity and antiproliferation to HepG2 of wheat bran was investigated. Moderate SFE treatment significantly enhanced the total soluble phenolic content of wheat bran. After SFE pretreatment, the free and conjugated ferulic acid content in the wheat bran were significantly increased. Antioxidant activities of SFE treated wheat bran were higher than those untreated wheat bran. The cellular antioxidant and antiproliferative activities of SFE treated wheat bran were also significantly ameliorated...
July 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27757749/effects-of-alkaloid-extracts-of-mesquite-pod-on-the-products-of-in-vitro-rumen-fermentation
#17
Taiala Cristina de Jesus Pereira, Mara Lúcia Albuquerque Pereira, Jeruzia Vitória Moreira, José Augusto Gomes Azevêdo, Ronan Batista, Vanderlúcia Fonseca de Paula, Brena Santos Oliveira, Edileusa de Jesus Dos Santos
The objective of this study was to evaluate the effects of alkaloid extracts of Prosopis juliflora (Sw.) D.C. pods obtained by two extraction methods as compared with sodium monensin on the gas production kinetic, mitigation of methane, and rumen fermentation products using wheat bran or Tifton 85 hay as substrates, by the semi-automatic in vitro gas production technique. A completely randomized design was adopted, and two natural additives were tested made from mesquite pod (alkaloid extract I and alkaloid extract II) at three levels (3...
October 18, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27744696/soluble-dietary-fiber-fractions-in-wheat-bran-and-their-interactions-with-wheat-gluten-have-impacts-on-dough-properties
#18
Qian Li, Rui Liu, Tao Wu, Min Zhang
Six soluble dietary fiber (SDF) fractions were prepared via stepwise ethanol precipitation from natural and fermented wheat bran. The chemical composition, molecular weight distribution, glycosidic linkage and substitution pattern of the SDF fractions were elucidated by sugar analysis, periodate oxidation and Smith degradation, molecular determination, and 1H nuclear magnetic resonance (NMR) analysis. The impacts of SDF fractions on the rheological properties and morphologies of doughs were investigated by farinography, rheometry and scanning electron microscopy (SEM) to clarify the relationship between the microstructural features of SDF fractions and the macroscopic properties of SDF-containing doughs...
October 17, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27744242/mycothermus-thermophilus-syn-scytalidium-thermophilum-repertoire-of-a-diverse-array-of-efficient-cellulases-and-hemicellulases-in-the-secretome-revealed
#19
Neha Basotra, Baljit Kaur, Marcos Di Falco, Adrian Tsang, Bhupinder Singh Chadha
Mycothermus thermophilus (Syn. Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium. The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied. Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present...
December 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27734757/agrowaste-to-vanillin-conversion-by-a-natural-pediococcus-acidilactici-strain-bd16
#20
Debkumar Chakraborty, Baljinder Kaur, Karthikeyan Obulisamy, Ammaiyappan Selvam, Jonathan W C Wong
Owing to its flavoring, antimicrobial, antioxidant and anticarcinogenic nature, vanillin is widely used in foods, beverages, perfumes and pharmaceutical products. Ferulic acid (FA) is an important precursor of vanillin which is abundant in cereals like maize, rice and wheat and sugar beet. A major drawback of microbial vanillin production from FA is the degradation and biotransformation of toxic vanillin to other phenolic derivatives. The present study is undertaken to explore microbial vanillin production from FA precursor rice bran by employing vanillin-resistant Pediococcus acidilactici BD16, a natural lactic acid bacteria isolate...
October 13, 2016: Environmental Technology
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