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glycosaminoglycan

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https://www.readbyqxmd.com/read/28731675/sustained-release-of-a-peptide-based-matrix-metalloproteinase-2-inhibitor-to-attenuate-adverse-cardiac-remodeling-and-improve-cardiac-function-following-myocardial-infarction
#1
Zhaobo Fan, Minghuan Fu, Zhaobin Xu, Bo Zhang, Zhihong Li, Haichang Li, Xinyu Zhou, Xuanyou Liu, Yunyan Duan, Pei-Hui Lin, Pu Duann, Xiaoyun Xie, Jianjie Ma, Zhenguo Liu, Jianjun Guan
Following myocardial infarction (MI), degradation of extracellular matrix (ECM) by upregulated matrix metalloproteinases (MMPs) especially MMP-2 decreases tissue mechanical properties, leading to cardiac function deterioration. Attenuation of cardiac ECM degradation at the early stage of MI has the potential to preserve tissue mechanical properties, resulting in cardiac function increase. Yet the strategy for efficiently preventing cardiac ECM degradation remains to be established. Current preclinical approaches have shown limited efficacy due to low drug dosage allocated to the heart tissue, dose-limiting side effects, and cardiac fibrosis...
July 21, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28730833/short-term-administration-of-melatonin-or-ghrelin-on-diabetic-rats-effects-on-angiotensin-ii-and-vasopressin-induced-uterine-contractility
#2
D Sugár, R Agócs, E Tatár, G Tóth, P Horváth, E Sulyok, A J Szabó
The glycosaminoglycan (GAG) molecules are a group of high molecular weight, negatively charged polysaccharides present abundantly in the mammalian organism. By their virtue of ion and water binding capacity, they may affect the redistribution of body fluids and ultimately the blood pressure. Data from the literature suggests that the mitogens Vascular Endothelial Growth Factor (VEGF)-A and VEGF-C are able to regulate the amount and charge density of GAGs and their detachment from the cell surface. Based on these findings we investigated the relationship between the level of dietary sodium intake, the expression levels of VEGF-A and VEGF-C, and the amount of the skin GAGs hyaluronic acid and chondroitin sulphate in an in vivo rat model...
July 18, 2017: Physiological Research
https://www.readbyqxmd.com/read/28729949/modeling-the-effect-of-blunt-impact-on-mitochondrial-function-in-cartilage-implications-for-development-of-osteoarthritis
#3
Georgi I Kapitanov, Bruce P Ayati, James A Martin
OBJECTIVE: Osteoarthritis (OA) is a disease characterized by degeneration of joint cartilage. It is associated with pain and disability and is the result of either age and activity related joint wear or an injury. Non-invasive treatment options are scarce and prevention and early intervention methods are practically non-existent. The modeling effort presented in this article is constructed based on an emerging biological hypothesis-post-impact oxidative stress leads to cartilage cell apoptosis and hence the degeneration observed with the disease...
2017: PeerJ
https://www.readbyqxmd.com/read/28723112/surfen-assembled-graphene-oxide-for-fluorescence-turn-on-detection-of-sulfated-glycosaminoglycans-in-biological-matrix
#4
Yen-Ting Wang, Wei-Lung Tseng
Sulfated glycosaminoglycans (GAGs) not only serve as a biomarker for mucopolysaccharidoses disease but also participate in various biological processes, such as blood clot medication (heparin) and signal transduction (heparan sulfate). However, few fluorescent sensors, such as 1,9-dimethylmethylene blue, have been developed for the detection of sulfated GAGs in the real world. Herein, we fabricated a surfen/few-layer graphene oxide (FLGO) nanocomplex for sensing sulfated GAGs in biological fluids. Surfen molecules are self-assembled onto the surface of FLGO through electrostatic attraction, and their fluorescence was then quenched by the creation of the FLGO-surfen complex (static quenching) and partially combined with the energy transfer from surfen to FLGO (dynamic quenching)...
June 23, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28721664/reciprocal-influence-of-hmscs-hacat-cultivated-on-electrospun-scaffolds
#5
Sirsendu Bhowmick, Sandra Rother, Heike Zimmermann, Poh S Lee, Stephanie Moeller, Matthias Schnabelrauch, Veena Koul, Dieter Scharnweber
Here, we investigated the synergistic effect of electrospun nanofibrous scaffolds made of gelatin /sulfated hyaluronan (sHA) or native hyaluronan (HA)/chondroitin sulfate (CS) and, keratinocytes (HaCaT)-human mesenchymal stem cells (hMSCs) contact co-culture on epithelial differentiation of hMSCs. The hMSCs were co-cultured in contact with HaCaT cells for 5 days on electrospun scaffold. Results show that electrospun scaffolds containing sulfated glycosaminoglycans (GAGs) stimulate epithelial differentiation in terms of various protein expression markers (keratin 14, ΔNp63α and Pan-cytokeratin) and gene expression of several dermal proteins (keratin 14, ΔNp63α)...
August 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28721300/extracellular-matrix-alterations-in-the-peyronie-s-disease
#6
Marcelo Silva Watanabe, Thérèse Rachel Theodoro, Natália Lima Coelho, Aline Mendes, Monica Luzia Pereira Leonel, Ana Maria Mader, Helena Bonciani Nader, Sidney Glina, Maria Aparecida Silva Pinhal
Peyronie's disease is characterized by fibrous plaque formation of the tunica albuginea, causing penile deformity and fertility problems. The aim of the present study was to investigate alterations in the extracellular matrix in Peyronie's disease. The study used tissues collected by surgical procedure from individuals that presented a well-established disease, while control samples were obtained by biopsies of fresh cadavers. Immunohistochemistry analysis followed by digital quantification was performed to evaluate TGF-β, heparanases and metalloproteinases (MMPs)...
July 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28720847/the-glycointeractome-of-serogroup-b-neisseria-meningitidis-strain-mc58
#7
Tsitsi D Mubaiwa, Lauren E Hartley-Tassell, Evgeny A Semchenko, Freda E-C Jen, Yogitha N Srikhanta, Christopher J Day, Michael P Jennings, Kate L Seib
Neisseria meningitidis express numerous virulence factors that enable it to interact with diverse microenvironments within the host, during both asymptomatic nasopharyngeal colonization and invasive disease. Many of these interactions involve bacterial or host glycans. In order to characterise the meningococcal glycointeractome, glycan arrays representative of structures found on human cells, were used as a screening tool to investigate host glycans bound by N. meningitidis. Arrays probed with fluorescently labelled wild-type MC58 revealed binding to 223 glycans, including blood group antigens, mucins, gangliosides and glycosaminoglycans...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28716818/intimal-hyperplasia-induced-by-vascular-intervention-causes-lipoprotein-retention-and-accelerated-atherosclerosis
#8
Siavash Kijani, Ana Maria Vázquez, Malin Levin, Jan Borén, Per Fogelstrand
Accelerated atherosclerosis diminishes the long term patency of vascular interventions, such as percutaneous coronary intervention and implantation of saphenous vein grafts. However, the cause of this accelerated atherosclerosis is unclear. In this study, we tested the hypothesis that intimal hyperplasia formed following vascular intervention promotes retention of atherogenic lipoproteins. Intimal hyperplasia was surgically induced in the mouse common carotid artery. The surgery was combined with different mouse models of hypercholesterolemia to obtain different cholesterol levels and to control the onsets of hypercholesterolemia...
July 2017: Physiological Reports
https://www.readbyqxmd.com/read/28715962/in-vitro-analysis-of-the-differentiation-capacity-of-postmortally-isolated-human-chondrocytes-influenced-by-different-growth-factors-and-oxygen-levels
#9
Anika Jonitz-Heincke, Annett Klinder, Diana Boy, Achim Salamon, Doris Hansmann, Juliane Pasold, Andreas Buettner, Rainer Bader
Objective In the present in vitro study, we analyzed the chondrogenic differentiation capacity of human chondrocytes postmortally isolated from unaffected knee cartilage by the addition of transforming growth factor-β1 (TGF-β1) and/or insulin-like growth factor-1 (IGF-1) and different oxygen levels. Design After 14 and 35 days, DNA concentrations and protein contents of Col1, Col2, aggrecan as well as glycosaminoglycans (GAGs) of chondrocytes cultivated as pellet cultures were analyzed. Additionally, expression rates of mesenchymal stem cell (MSC)-associated differentiation markers were assessed in monolayer cultures...
July 1, 2017: Cartilage
https://www.readbyqxmd.com/read/28713940/a-novel-method-to-identify-hub-pathways-of-rheumatoid-arthritis-based-on-differential-pathway-networks
#10
Shi-Tong Wei, Yong-Hua Sun, Shi-Hua Zong
The aim of the current study was to identify hub pathways of rheumatoid arthritis (RA) using a novel method based on differential pathway network (DPN) analysis. The present study proposed a DPN where protein‑protein interaction (PPI) network was integrated with pathway‑pathway interactions. Pathway data was obtained from background PPI network and the Reactome pathway database. Subsequently, pathway interactions were extracted from the pathway data by building randomized gene‑gene interactions and a weight value was assigned to each pathway interaction using Spearman correlation coefficient (SCC) to identify differential pathway interactions...
July 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28709984/micrometer-scale-guidance-of-mesenchymal-stem-cells-to-form-structurally-oriented-large-scale-tissue-engineered-cartilage
#11
Chih-Ling Chou, Alexander L Rivera, Valencia Williams, Jean F Welter, Joseph M Mansour, Judith A Drazba, Takao Sakai, Harihara Baskaran
Current clinical methods to treat articular cartilage lesions provide temporary relief of the symptoms but fail to permanently restore the damaged tissue. Tissue engineering, using mesenchymal stem cells (MSCs) combined with scaffolds and bioactive factors, is viewed as a promising method for repairing cartilage injuries. However, current tissue engineered constructs display inferior mechanical properties compared to native articular cartilage, which could be attributed to the lack of structural organization of the extracellular matrix (ECM) of these engineered constructs in comparison to the highly oriented structure of articular cartilage ECM...
July 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28708486/improved-quality-of-porcine-embryos-cultured-with-hyaluronan-due-to-the-modification-of-the-mitochondrial-membrane-potential-and-reactive-oxygen-species-level
#12
Marek Romek, Barbara Gajda, Ewa Krzysztofowicz, Marcin Kucia, Agnieszka Uzarowska, Zdzislaw Smorag
Although considerable progress has been made in pig embryo culture systems, the developmental competence and quality of the produced embryos are still lower than their in vivo-derived counterparts. Because hyaluronan (HA) regulates various cellular processes and possesses antioxidant properties, this glycosaminoglycan seems to be a promising supplement in culture media. However, until now, its beneficial influence on in vitro pig embryo development has been debatable. Hence, we aimed to investigate the effect of 0...
June 29, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708257/aquaporin1-and-3-modification-as-a-result-of-chondrogenic-differentiation-of-human-mesenchymal-stem-cell
#13
Adriana Carol Eleonora Graziano, Rosanna Avola, Giovanna Pannuzzo, Venera Cardile
Chondrocytes are cells of articular cartilage particularly sensitive to water transport and ionic and osmotic changes from extracellular environment and responsible for the production of the synovial fluid. Aquaporins (AQPs) are a family of water and small solute transport channel proteins identified in several tissues, involved in physiological pathways and in manifold human diseases. In a recent period, AQP1 and 3 seem to have a role in metabolic water regulation in articular cartilage of load bearing joints...
July 14, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28706280/radiation-induced-changes-in-the-glycome-of-endothelial-cells-with-functional-consequences
#14
Cyprien Jaillet, Willy Morelle, Marie-Christine Slomianny, Vincent Paget, Georges Tarlet, Valérie Buard, Sonia Selbonne, Fanny Caffin, Emilie Rannou, Pierre Martinez, Agnès François, François Foulquier, Fabrice Allain, Fabien Milliat, Olivier Guipaud
As it is altered by ionizing radiation, the vascular network is considered as a prime target in limiting normal tissue damage and improving tumor control in radiation therapy. Irradiation activates endothelial cells which then participate in the recruitment of circulating cells, especially by overexpressing cell adhesion molecules, but also by other as yet unknown mechanisms. Since protein glycosylation is an important determinant of cell adhesion, we hypothesized that radiation could alter the glycosylation pattern of endothelial cells and thereby impact adhesion of circulating cells...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28706183/modulation-of-interleukin-12-activity-in-the-presence-of-heparin
#15
Srinivas Jayanthi, Bhanu Prasanth Koppolu, Khue G Nguyen, Sean G Smith, Barbara K Felber, Thallapuranam Krishnaswamy Suresh Kumar, David A Zaharoff
Glycosaminoglycans (GAGs), especially heparin and heparan sulfate (HS), modulate the functions of numerous cytokines. The aims of this multidisciplinary research were to characterize heparin binding to interleukin-12 (IL-12) and determine the mechanism(s) by which heparin influences IL-12 bioactivity. Heparin and HS were found to bind human IL-12 (hIL-12) with low micromolar affinity and increase hIL-12 bioactivity by more than 6-fold. Conversely, other GAGs did not demonstrate significant binding, nor did their addition affect hIL-12 bioactivity...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28704566/development-and-characterization-of-a-human-three-dimensional-chondrosarcoma-culture-for-in-vitro-drug-testing
#16
Aurélien Voissiere, Elodie Jouberton, Elise Maubert, Françoise Degoul, Caroline Peyrode, Jean-Michel Chezal, Élisabeth Miot-Noirault
It has been suggested that chemoresistance of chondrosarcoma (CHS), the cartilage tumor, is caused by the phenotypic microenvironmental features of the tumor tissue, mainly the chondrogenic extracellular matrix (ECM), and hypoxia. We developed and characterized a multicellular tumor spheroid (MCTS) of human chondrosarcoma HEMC-SS cells to gain insight into tumor cell biology and drug response. At Day 7, HEMC-SS spheroids exhibited a homogeneous distribution of proliferative Ki-67 positive cells, whereas in larger spheroids (Day 14 and Day 20), proliferation was mainly localized in the periphery...
2017: PloS One
https://www.readbyqxmd.com/read/28704392/redifferentiation-of-aged-human-articular-chondrocytes-by-combining-bone-morphogenetic-protein-2-and-melanoma-inhibitory-activity-protein-in-3d-culture
#17
Stephan Payr, Brigitte Tichy, Clemens Atteneder, Marc Michel, Thomas Tiefenboeck, Nikolaus Lang, Sylvia Nuernberger, Stefan Hajdu, Elizabeth Rosado-Balmayor, Stefan Marlovits, Christian Albrecht
Melanoma inhibitory activity (MIA) affects the differentiation to hyaline cartilage and can inhibit the osteogenic potential of bone morphogenetic protein (BMP)-2 in mesenchymal stem cells (MSC). The aim of this study was to investigate if MIA also inhibits the osteogenic potential of BMP-2 in human articular chondrocytes during redifferentiation, which may lead to a higher grade of differentiation without calcification. HAC of four female patients (mean age: 73.75 ±6.98) were seeded into 3D culture for 28 days; after adding the recombinant proteins, four groups were formed (Control, BMP-2, MIA, BMP-2+MIA)...
2017: PloS One
https://www.readbyqxmd.com/read/28702876/cardiovascular-histopathology-of-a-11-year-old-with-mucopolysaccharidosis-vii-demonstrates-fibrosis-macrophage-infiltration-and-arterial-luminal-stenosis
#18
Valerie Lew, Louis Pena, Robert Edwards, Raymond Y Wang
Mucopolysaccharidosis type VII (MPS VII) is caused by β-glucuronidase deficiency, resulting in lysosomal accumulation of glycosaminoglycans (GAGs) and multisystemic disease. We present cardiovascular gross and histopathology findings from a 11-year-old MPS VII male, who expired after developing ventricular fibrillation following anesthesia induction. Gross anatomic observations were made at autopsy; postmortem formalin-fixed paraffin-embedded samples of the carotid artery, aorta, myocardium, and valves were sectioned and stained with hematoxylin-eosin, Verhoeff-Van Gieson, CD68, and trichrome stains...
July 13, 2017: JIMD Reports
https://www.readbyqxmd.com/read/28702111/innate-function-of-house-dust-mite-allergens-robust-enzymatic-degradation-of-extracellular-matrix-at-elevated-ph
#19
Kumiko Oida, Lukas Einhorn, Ina Herrmann, Lucia Panakova, Yvonne Resch, Susanne Vrtala, Gerlinde Hofstetter, Akane Tanaka, Hiroshi Matsuda, Erika Jensen-Jarolim
BACKGROUND: Exposure to the house dust mite Dermatophagoides pteronyssinus (D.p.) increases the risk for developing allergic diseases in humans and their best friends, the dogs. Here, we explored whether this allergenic mite via its enzymes may impact the cutaneous extracellular matrix (ECM), which critically determines epithelial barrier integrity both structurally and functionally. METHODS: Two extracts obtained from either dust-purified or cultured D.p. bodies were used in the present study...
2017: World Allergy Organization Journal
https://www.readbyqxmd.com/read/28700908/endothelial-glycocalyx-mediated-nitric-oxide-production-in-response-to-selective-afm-pulling
#20
Anne Marie W Bartosch, Rick Mathews, John M Tarbell
Nitric oxide (NO) is a regulatory molecule in the vascular system and its inhibition due to endothelial injury contributes to cardiovascular disease. The glycocalyx is a thin layer of glycolipids, glycoproteins, and proteoglycans on the surface of mammalian epithelial cells. Extracellular forces are transmitted through the glycocalyx to initiate intracellular signaling pathways. In endothelial cells (ECs), previous studies have shown the glycocalyx to be a significant mediator of NO production; degradation of the endothelial glycocalyx layer (EGL) drastically reduces EC production of NO in response to fluid shear stress...
July 11, 2017: Biophysical Journal
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