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https://www.readbyqxmd.com/read/29791480/complex-interactions-in-a-novel-scn5a-compound-mutation-associated-with-long-qt-and-brugada-syndrome-implications-for-na-channel-blocking-pharmacotherapy-for-de-novo-conduction-disease
#1
Jie Liu, Jason D Bayer, Roozbeh Aschar-Sobbi, Marianne Wauchop, Danna Spears, Michael Gollob, Edward J Vigmond, Robert Tsushima, Peter H Backx, Vijay S Chauhan
BACKGROUND: The SCN5A mutation, P1332L, is linked to a malignant form of congenital long QT syndrome, type 3 (LQT3), and affected patients are highly responsive to the Na+ channel blocking drug, mexiletine. In contrast, A647D is an atypical SCN5A mutation causing Brugada syndrome. An asymptomatic male with both P1332L and A647D presented with varying P wave/QRS aberrancy and mild QTc prolongation which did not shorten measurably with mexiletine. OBJECTIVE: We characterized the biophysical properties of P1332L, A647D and wild-type (WT) Na+ channels as well as their combinations in order to understand our proband's phenotype and to guide mexilitine therapy...
2018: PloS One
https://www.readbyqxmd.com/read/29788776/lenstar%C3%A2-ls-900-vs-pentacam%C3%A2-axl-comparative-study-of-ocular-biometric-measurements-and-intraocular-lens-power-calculation
#2
Joana Maria Mendes Pereira, Arminda Neves, Pedro Alfaiate, Mónica Santos, Henrique Aragão, João C Sousa
PURPOSE: Comparison of biometric measurements and calculation of intraocular lens with a new biometer (Pentacam®-AXL, Oculus, Germany) and a reference biometer (Lenstar LS 900®, Haag-Streit AG, Switzerland), in order to assess the agreement between these two devices. SETTING: Centro Hospitalar de Leiria, Portugal Materials and methods: Prospective, institutional study, in which measurements of axial length, anterior chamber depth from the corneal epithelium and endothelium to the anterior surface of the lens (anterior chamber depth ext and anterior chamber depth int), central corneal thickness and keratometry readings of the flattest and steepest meridians (K1 and K2) were obtained with the two systems...
May 1, 2018: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/29787928/principle-of-k-na-selectivity-in-the-active-site-of-group-ii-intron-at-various-stages-of-self-splicing-pathway
#3
Abhishek Kumar, Priyadarshi Satpati
Group II introns are ribozymes which can catalyze its own splicing and relegate itself. They share common structural features and are evolutionarily related to eukaryotic spliceosome. Hence, group II introns are an excellent model system for understanding the mechanism of RNA-splicing in gene expression. Recent advancement of structural studies has provided x-ray structures of group II intron at different stages (Pre-hydrolytic, post-hydrolytic and free intron) of splicing pathway and revealed heteronuclear metal ion cluster (two potassium; K1 and K2, two magnesium; M1 and M2) in the active site as common structural feature of group II introns...
May 14, 2018: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29781908/musculoskeletal-injury-in-paddle-sport-athletes
#4
Andrew R Griffin, Diana M Perriman, Teresa M Neeman, Paul N Smith
INTRODUCTION: Kayak racing has been an Olympic sport since 1936. The sport is evolving with the introduction of ocean skis and stand-up-paddle boards (SUP). Musculoskeletal injury incidence surveys have been conducted for ultra-marathon events, but no data have been published for other racing formats. OBJECTIVE: To identify and compare the rates and types of injuries sustained by paddling athletes as a function of discipline and training parameters in Sprint, Marathon, Ultra-Marathon, and Ocean events...
May 16, 2018: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
https://www.readbyqxmd.com/read/29778454/influence-of-the-beam-profile-crosslinking-setting-on-changes-in-corneal-topography-and-tomography-in-progressive-keratoconus-preliminary-results
#5
Robert Herber, Kathleen S Kunert, Věra Veliká, Eberhard Spoerl, Lutz E Pillunat, Frederik Raiskup
PURPOSE: To evaluate the effect of accelerated corneal crosslinking (CXL) with a "higher peripheral intensity" profile setting compared with a standard "top hat" profile setting performed using different CXL systems. SETTING: Department of Ophthalmology, University Hospital Carl Gustav Carus, Dresden, Germany. DESIGN: Retrospective case series. METHODS: Eyes from patients with progressive keratoconus were included...
May 16, 2018: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/29777593/degradation-of-recombinant-proteins-by-cho-host-cell-proteases-is-prevented-by-matriptase-1-knock-out
#6
Holger Laux, Sandrine Romand, Sandro Nuciforo, Christopher J Farady, Joel Tapparel, Stine Buechmann-Moeller, Benjamin Sommer, Edward J Oakeley, Ursula Bodendorf
An increasing number of non-antibody format proteins are entering the clinical development. However, one of the major hurdles for the production of non-antibody glycoproteins is host cell-related proteolytic degradation, which can drastically impact developability and timelines of pipeline projects. Chinese hamster ovary (CHO) cells are the preferred production host for recombinant therapeutic proteins. Using protease inhibitors, transcriptomics and genetic knockdowns we have identified, out of the more than 700 known proteases in rodents, Matriptase-1 as the major protease involved in degradation of recombinant proteins expressed in CHO-K1 cells...
May 19, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29772356/assessment-of-toxicity-and-differential-antimicrobial-activity-of-methanol-extract-of-rhizome-of-simaba-ferruginea-a-st-hil-and-its-isolate-canthin-6-one
#7
Vanessa Fátima Gazoni, Sikiru Olaitan Balogun, Karuppusamy Arunacham, Darley Maria Oliveira, Valdir Cechinel Filho, Samara Rosolem Lima, Edson Moleta Colodel, Ilsamar Mendes Soares, Sérgio Donizeti Ascêncio, Domingos Tabajara de Oliveira Martins
ETHNOPHARMACOLOGICAL RELEVANCE: Simaba ferruginea A. St.-Hil., Simaroubaceae, popularly known as "calunga" is a typical subtropical shrub used in Central Brazil mainly for infection, anti-inflammatory, analgesic and gastric duodenal-ulcers. It presents in its composition the alkaloid canthin-6-one, an alkaloid indole β-carboxylic. AIM: This study aims to investigate the toxicity, antimicrobial activities of methanol extract of Simaba ferruginea (MESf) and canthin-6-one by using different experimental models...
May 14, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29772212/a-new-microcystin-producing-nostoc-strain-discovered-in-broad-toxicological-screening-of-non-planktic-nostocaceae-cyanobacteria
#8
Andreja Kust, Petra Urajová, Pavel Hrouzek, Dai Long Vu, Kateřina Čapková, Lenka Štenclová, Klára Řeháková, Eliška Kozlíková-Zapomělová, Olga Lepšová-Skácelová, Alena Lukešová, Jan Mareš
Benthic cyanobacteria recognized as producers of natural products, including cyanotoxins, have been neglected for systematic toxicological studies. Thus, we have performed a broad study investigating cyanotoxin potential of 311 non-planktic nostocacean representatives combining molecular and chemical analyses. Out of these, a single strain Nostoc sp. Treb K1/5, was identified as a new microcystin producer. Microcystins [Asp3 ]MC-YR, [Asp3 ]MC-FR, [Asp3 ]MC-HtyR and Ala-Leu/Ile-Asp-Arg-Adda-Glu-Mdha are reported for the first time from the genus Nostoc...
May 14, 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29771883/-transepithelial-corneal-collagen-crosslinking-in-patients-with-progressive-keratoconus
#9
A I Kopaenko, N V Ivanova
PURPOSE: To assess the effectiveness of transepithelial collagen crosslinking (CXL) in patients with keratoconus. MATERIAL AND METHODS: The study involved 44 patients (44 eyes) with stage I-II progressive keratoconus. Patients were divided into two groups. The 1st group included 22 patients that had underwent transepithelial CXL. The 2nd group included 22 patients that had underwent traditional CXL. RESULTS AND DISCUSSION: All patients of the group 2 all patients had corneal syndrome, which lasted 2-4 days...
2018: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/29762913/an-online-compendium-of-cho-rna-seq-data-allows-identification-of-cho-cell-line-specific-transcriptomic-signatures
#10
Ankita Singh, Helene F Kildegaard, Mikael R Andersen
Chinese hamster ovary (CHO) cell lines can fold, assemble and modify proteins post-translationally to produce human-like proteins; as a consequence, it is the single most common expression systems for industrial production of recombinant therapeutic proteins. A thorough knowledge of cultivation conditions of different CHO cell lines has been developed over the last decade, but comprehending gene or pathway-specific distinctions between CHO cell lines at transcriptome level remains a challenge. To address these challenges, we compiled a compendium of 23 RNA-Seq studies from public and in-house data on CHO cell lines, i...
May 15, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29758033/phylogenetic-relationships-biofilm-formation-motility-antibiotic-resistance-and-extended-virulence-genotypes-among-escherichia-coli-strains-from-women-with-community-onset-primitive-acute-pyelonephritis
#11
Arianna Pompilio, Valentina Crocetta, Vincenzo Savini, Dezemona Petrelli, Marta Di Nicola, Silvia Bucco, Luigi Amoroso, Mario Bonomini, Giovanni Di Bonaventura
The present work set out to search for a virulence repertoire distinctive for Escherichia coli causing primitive acute pyelonephritis (APN). To this end, the virulence potential of 18 E. coli APN strains was genotypically and phenotypically assessed, comparatively with 19 strains causing recurrent cystitis (RC), and 16 clinically not significant (control, CO) strains. Most of the strains belong to phylogenetic group B1 (69.8%; p<0.01), and APN strains showed unique features, which are the presence of phylogroup A, and the absence of phylogroup B2 and non-typeable strains...
2018: PloS One
https://www.readbyqxmd.com/read/29757586/production-of-recombinant-protein-of-salsola-kali-sal-k1-pollen-allergen-in-lactococcus-lactis
#12
Ziba Ghasemi, Abdol-Reza Varasteh, Malihe Moghadam, Farnaz Sedghi, Farahzad Jabbariazad, Mojtaba Sankian
The Salsola kali pollen is considered the main cause of allergic sensitization in desert and semi-desert regions. We have constructed recombinant Lactococcus lactis producing Sal k1 protein with the aim of using it as a mucosal vaccine for specific immunotherapy. The Sal k1 gene was amplified, and transferred into a PNZ 8148 plasmid. The PNZ8148-Sal k1 recombinant plasmid was transformed into competent E.coli strain MC1061 for replication, and then was isolated and cloned into competent L. lactis by electroporation...
April 2018: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29756273/expanding-the-sar-of-non-toxic-antiplasmodial-indolyl-3-ethanone-ethers-and-thioethers
#13
Mayibongwe J Lunga, Ruramai L Chisango, Carli Weyers, Michelle Isaacs, Dale Taylor, Adrienne L Edkins, Setshaba D Khanye, Heinrich C Hoppe, Clinton Veale
Despite major strides in reducing Plasmodium falciparum infections, this parasite still accounts for roughly half a million annual deaths. This problem is compounded by reduced efficacy of artemisinin combination therapies (ACT). Therefore, the development and optimisation of novel antimalarial chemotypes is critical. In this study, we describe our strategic approach to optimise a class of previously reported antimalarials, resulting in the discovery compounds 13 and 14 whose activity was equipotent to chloroquine against the P...
May 13, 2018: ChemMedChem
https://www.readbyqxmd.com/read/29746106/improved-efficacy-of-synthesizing-m-iii-labeled-dota-complexes-in-binary-mixtures-of-water-and-organic-solvents-a-combined-radio-and-physicochemical-study
#14
Marylaine Pérez-Malo, Gergely Szabó, Elisabeth Eppard, Adrienn Vagner, Ernő Brücher, Imre Tóth, Alessandro Maiocchi, Eul Hyun Suh, Zoltán Kovács, Zsolt Baranyai, Frank Rösch
Typically, the synthesis of radiometal-based radiopharmaceuticals is performed in buffered aqueous solutions. We found that the presence of organic solvents like ethanol increased the radiolabeling yields of [68 Ga]Ga-DOTA (DOTA = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacatic acid). In the present study, the effect of organic cosolvents [ethanol (EtOH), isopropyl alcohol, and acetonitrile] on the radiolabeling yields of the macrocyclic chelator DOTA with several trivalent radiometals (gallium-68, scandium-44, and lutetium-177) was systematically investigated...
May 10, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29744043/genome-based-analysis-of-carbapenemase-producing-klebsiella-pneumoniae-isolates-from-german-hospital-patients-2008-2014
#15
Laura Becker, Martin Kaase, Yvonne Pfeifer, Stephan Fuchs, Annicka Reuss, Anja von Laer, Muna Abu Sin, Miriam Korte-Berwanger, Sören Gatermann, Guido Werner
Background: By using whole genome sequence data we aimed at describing a population snapshot of carbapenemase-producing K. pneumoniae isolated from hospitalized patients in Germany between 2008 and 2014. Methods: We selected a representative subset of 107 carbapenemase-producing K. pneumoniae clinical isolates possessing the four most prevalent carbapenemase types in Germany (KPC-2, KPC-3, OXA-48, NDM-1). Isolates were processed via illumina NGS. Data were analysed using different SNP-based mapping and de-novo assembly approaches...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29741057/-effects-of-different-potassium-levels-on-growth-and-no-3-uptake-and-utilization-of-malus-hupehensis-seedlings
#16
Ge Tian, Fen Wang, Ling Peng, Liu He, Yuan Mao Jiang, Shun Feng Ge
In order to explore the effects of potassium fertilizer on the absorption and utilization of nitrogen fertilizer, and provide theoretical basis for scientific fertilization in apple orchard, Malus hupehensis seedlings were used to study the effects of different potassium levels (K0 , K1 , K2 , K3 , K4 , K5 , and K6 equivalent to 0, 2, 4, 6, 8, 10, and 12 mmol·L-1 K+ , respectively) on growth and NO3 - uptake and utilization using the track technology of 15 N and non-damage microtest technique. The results showed that root vigor, nitrate reductase activity and root morphological index of seedlings in K3 treatment were significantly higher than those in the other treatments...
July 18, 2017: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/29739105/blood-donation-are-we-willing-a-cross-sectional-study-among-dental-students
#17
Dolar Doshi, P Karunakar, D Satyanarayana, B Srikanth Reddy
BACKGROUND: To assess the knowledge, attitude, and practice towards blood donation among a cohort of dental students. METHODS: This cross-sectional study included all the undergraduate and post-graduate dental students of Panineeya Institute of Dental Sciences and Hospital. A self-administered questionnaire comprised of four parts. The first part gathered socio-demographic data. The subsequent parts consisted of 11-knowledge questions (K1-11), 11attitude questions (A1-11), and the fourth part evaluated the practice of blood donation (P1-11)...
March 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29735656/suppressor-mutation-analysis-combined-with-3d-modeling-explains-cohesin-s-capacity-to-hold-and-release-dna
#18
Xingya Xu, Ryuta Kanai, Norihiko Nakazawa, Li Wang, Chikashi Toyoshima, Mitsuhiro Yanagida
Cohesin is a fundamental protein complex that holds sister chromatids together. Separase protease cleaves a cohesin subunit Rad21/SCC1, causing the release of cohesin from DNA to allow chromosome segregation. To understand the functional organization of cohesin, we employed next-generation whole-genome sequencing and identified numerous extragenic suppressors that overcome either inactive separase/Cut1 or defective cohesin in the fission yeast Schizosaccharomyces pombe Unexpectedly, Cut1 is dispensable if suppressor mutations cause disorders of interfaces among essential cohesin subunits Psm1/SMC1, Psm3/SMC3, Rad21/SCC1, and Mis4/SCC2, the crystal structures of which suggest physical and functional impairment at the interfaces of Psm1/3 hinge, Psm1 head-Rad21, or Psm3 coiled coil-Rad21...
May 7, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29732524/integrated-analysis-of-dynamic-fet-pet-ct-parameters-histology-and-methylation-profiling-of-44-gliomas
#19
Manuel Röhrich, Kristin Huang, Daniel Schrimpf, Nathalie L Albert, Thomas Hielscher, Andreas von Deimling, Ulrich Schüller, Antonia Dimitrakopoulou-Strauss, Uwe Haberkorn
PURPOSE: Dynamic 18 F-FET PET/CT is a powerful tool for the diagnosis of gliomas.18 F-FET PET time-activity curves (TAC) allow differentiation between histological low-grade gliomas (LGG) and high-grade gliomas (HGG). Molecular methods such as epigenetic profiling are of rising importance for glioma grading and subclassification. Here, we analysed dynamic 18 F-FET PET data, and the histological and epigenetic features of 44 gliomas. METHODS: Dynamic 18 F-FET PET was performed in 44 patients with newly diagnosed, untreated glioma: 10 WHO grade II glioma, 13 WHO grade III glioma and 21 glioblastoma (GBM)...
May 7, 2018: European Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/29730503/fibulin-7-a-heparin-binding-matricellular-protein-promotes-renal-tubular-calcification-in-mice
#20
Jun Tsunezumi, Hidekazu Sugiura, Lalhaba Oinam, Aktar Ali, Bui Quoc Thang, Aiko Sada, Yoshito Yamashiro, Makoto Kuro-O, Hiromi Yanagisawa
Ectopic calcification occurs during development of chronic kidney disease and has a negative impact on long-term prognosis. The precise molecular mechanism and prevention strategies, however, are not established. Fibulin-7 (Fbln7) is a matricellular protein structurally similar to elastogenic short fibulins, shown to bind dental mesenchymal cells and heparin. Here, we report that Fbln7 is highly expressed in renal tubular epithelium in the adult kidney and mediates renal calcification in mice. In vitro analysis revealed that Fbln7 bound heparin at the N-terminal coiled-coil domain...
May 3, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
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