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https://www.readbyqxmd.com/read/29152418/generation-and-characterization-of-cross-neutralizing-human-monoclonal-antibody-against-4-serotypes-of-dengue-virus-without-enhancing-activity
#1
Subenya Injampa, Nataya Muenngern, Chonlatip Pipattanaboon, Surachet Benjathummarak, Khwanchit Boonha, Hathairad Hananantachai, Waranya Wongwit, Pongrama Ramasoota, Pannamthip Pitaksajjakul
Background: Dengue disease is a leading cause of illness and death in the tropics and subtropics. Most severe cases occur among patients secondarily infected with a different dengue virus (DENV) serotype compared with that from the first infection, resulting in antibody-dependent enhancement activity (ADE). Our previous study generated the neutralizing human monoclonal antibody, D23-1B3B9 (B3B9), targeting the first domain II of E protein, which showed strong neutralizing activity (NT) against all four DENV serotypes...
2017: PeerJ
https://www.readbyqxmd.com/read/29152257/a-new-model-for-evaluating-maintenance-energy-requirements-in-dogs-allometric-equation-from-319-pet-dogs
#2
Guilhem Divol, Nathalie Priymenko
Reports concerning maintenance energy requirements (MER) in dogs are common but most of the data cover laboratory or utility dogs. This study establishes those of healthy adult pet dogs and the factors which cause these energy requirements to vary. Within the framework of a nutrition teaching exercise, each student followed a pet from his entourage and gathered accurate records of its feeding habits. Data have been restricted to healthy adult dogs with an ideal body weight (BW) which did not vary more than 5 % during the study period...
2017: Journal of Nutritional Science
https://www.readbyqxmd.com/read/29142205/coupling-of-emergent-octahedral-rotations-to-polarization-in-k-na-nbo3-ferroelectrics
#3
I Levin, V Krayzman, G Cibin, M G Tucker, M Eremenko, K Chapman, R L Paul
Perovskite potassium sodium niobates, K1-xNaxNbO3, are promising lead-free piezoelectrics. Their dielectric and piezoelectric characteristics peak near x = 0.5, but the reasons for such property enhancement remain unclear. We addressed this uncertainty by analyzing changes in the local and average structures across the x = 0.5 composition, which have been determined using simultaneous Reverse Monte Carlo fitting of neutron and X-ray total-scattering data, potassium EXAFS, and diffuse-scattering patterns in electron diffraction...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29139561/the-novel-potent-and-highly-selective-5-ht4-receptor-agonist-yh12852-significantly-improves-both-upper-and-lower-gastrointestinal-motility
#4
Eun Jeong Jeong, Soo Yong Chung, Han Na Hong, Se-Woong Oh, Jae Young Sim
BACKGROUND AND PURPOSE: 5-HT4 receptor agonists have been shown to be effective to treat various gastrointestinal tract disorders. However, a lack of selectivity against off-targets has been a limiting factor. EXPERIMENTAL APPROACH: The binding affinity and selectivity of YH12852 for human 5-HT4(a) receptor in CHO-K1 cells were evaluated using radioligand binding assays, and agonistic activity was assessed using a beta-lactamase reporter system. Contractile activity and propulsive motility were measured in isolated guinea pig distal colon...
November 15, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29125165/syntheses-and-characterization-of-phosphonates-and-diphosphonates-of-molybdenum-a4-moo3-5-o3pr-2-%C3%A2-xh2o-a2-mo2o5-o3pr-2-and-a2-mo2o5-o3p-r-po3-a-k-rb-cs-tl-nh4
#5
D Elias Jesu Packiam, Kanamaluru Vidyasagar
Twenty new molybdenum phosphonates and diphosphonates have been synthesized and structurally characterized by single crystal and powder X-ray diffraction, CHN analyses, spectroscopic and thermal studies. Four of them are molecular phenyl- and benzyl-phosphonates containing discrete [(MoO3)5(O3PR)2](4-) (R = Ph or CH2Ph) cyclic anions. The sixteen non-molecular compounds are layered isostructural phenylphosphonates, A2[Mo2O5(O3PPh)2] (A = NH4, Tl, Rb, Cs) and K1.5(H3O)0.5[Mo2O5(O3PPh)2] and the corresponding diphosphonate compounds with pillared anionic layers, A2[Mo2O5(O3P(CH2)3PO3)], A2[Mo2O5(O3P(CH2)4PO3)] and A2[Mo2O5(O3P(C6H4)PO3)]...
November 10, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29120943/native-prion-protein-homodimers-are-destabilized-by-oligomeric-amyloid-%C3%AE-1-42-species-as-shown-by-single-molecule-imaging
#6
Sachin S Tiwari, Yuki M Shirai, Yuri L Nemoto, Kumiko Kojima, Kenichi G N Suzuki
Prion proteins (PrPc) are receptors for amyloid β 1-42 (Aβ1-42) oligomers, but we do not know the impact of Aβ1-42 binding to PrPc on the interaction of membrane-bound PrPc with molecules that regulate downstream biological pathways. Stability of the PrPc dimeric complex and subsequent intermolecular interactions with membranous or cytoplasmic molecules are important for physiological functions of PrPc including neuroprotection. The principal aim of this study was to determine whether homodimer lifetime of PrPc is affected by the presence of Aβ1-42 oligomers...
November 8, 2017: Neuroreport
https://www.readbyqxmd.com/read/29114443/evaluating-the-toxicity-of-tio2-based-nanoparticles-to-chinese-hamster-ovary-cells-and-escherichia-coli-a-complementary-experimental-and-computational-approach
#7
Alicja Mikolajczyk, Natalia Sizochenko, Ewa Mulkiewicz, Anna Malankowska, Michal Nischk, Przemyslaw Jurczak, Seishiro Hirano, Grzegorz Nowaczyk, Adriana Zaleska-Medynska, Jerzy Leszczynski, Agnieszka Gajewicz, Tomasz Puzyn
Titania-supported palladium, gold and bimetallic nanoparticles (second-generation nanoparticles) demonstrate promising photocatalytic properties. However, due to unusual reactivity, second-generation nanoparticles can be hazardous for living organisms. Considering the ever-growing number of new types of nanoparticles that can potentially contaminate the environment, a determination of their toxicity is extremely important. The main aim of presented study was to investigate the cytotoxic effect of surface modified TiO2-based nanoparticles, to model their quantitative nanostructure-toxicity relationships and to reveal the toxicity mechanism...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/29102678/a-monoclonal-antibody-raised-against-a-thermo-stabilised-%C3%AE-1-adrenoceptor-interacts-with-extracellular-loop-2-and-acts-as-a-negative-allosteric-modulator-of-a-sub-set-of-%C3%AE-1-adrenoceptors-expressed-in-stable-cell-lines
#8
Mark Soave, Gabriella Cseke, Catherine J Hutchings, Alastair J H Brown, Jeanette Woolard, Stephen J Hill
Recent interest has focused on antibodies that can discriminate between different receptor conformations. Here we have characterised the effect of a monoclonal antibody (mAb3), raised against a purified thermo-stabilised turkey β1-adrenoceptor (β1AR-m23 StaR), on β1-ARs expressed in CHO-K1 or HEK 293 cells. Immunohistochemical and radioligand-binding studies demonstrated that mAb3 was able to bind to ECL2 of the tβ1-AR, but not its human homologue. Specific binding of mAb3 to tβ1-AR was inhibited by a peptide based on the turkey, but not the human, ECL2 sequence...
November 1, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29100613/density-dependence-of-retention-factors-of-trans-stilbene-oxide-for-chiral-separation-by-supercritical-fluid-chromatography
#9
Toshitaka Funazukuri, Yuna Ono, Junichi Sakabe, Chang Yi Kong
Retention factors for two enantiomers of trans-stilbene oxide, k1 and k2, were measured with a chiral AD-H column using two syringe pumps to feed CO2 and methanol as a co-solvent at various temperatures, pressures and co-solvent mole fractions to determine the effects of these operating conditions on the retention factors. The retention factors k1 and k2 are for the (R,R)- and (S,S)-forms, respectively. When the isothermal compressibilities of a mixture of CO2 and MeOH were lower than 0.01, far from the critical locus of the CO2 and methanol mixture, both retention factors were well expressed with the solvent density and temperature with an average absolute relative deviation of 1-2%...
October 28, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29098938/stereochemical-assignment-of-the-unique-electron-acceptor-5-hydroxyphylloquinone-a-polar-analog-of-vitamin-k1-in-photosystem-i
#10
Makiko Kosugi, Changwoo Lee, Tomonori Misaki, Yasuhiro Kashino, Morifumi Fujita, Takashi Sugimura
A unique electron-accepting analog of vitamin K1 found in photosystem I in several species of oxygenic photosynthetic microorganisms was confirmed to be 5'-hydroxyphylloquinone (1) through stereo-uncontrolled synthesis. Furthermore, the stereochemistry of 1 obtained from Synechococcus sp. PCC 7942 was assigned to be 5'S using proline-catalyzed stereocontrolled reactions.
November 3, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29094808/dietary-vitamin-k-and-association-with-hepatic-vitamin-k-status-in-a-yup-ik-study-population-from-southwestern-alaska
#11
Nicholas T Au, Tove Ryman, Allan E Rettie, Scarlett E Hopkins, Bert B Boyer, Jynene Black, Jacques Philip, Joseph Yracheta, Alison E Fohner, Morayma Reyes, Timothy A Thornton, Melissa A Austin, Kenneth E Thummel
SCOPE: We investigated the relationship between dietary vitamin K and plasma PIVKA-II concentration, a biomarker of hepatic vitamin K status, in a Yup'ik study population in southwestern Alaska. METHODS AND RESULTS: A total of 659 male and female, self-reported Yup'ik people, ≥ 14 years of age, were enrolled. Blood was collected for genotyping and plasma PIVKA-II biomarker analysis. A Yup'ik-specific dietary food frequency questionnaire was used to assess vitamin K intake...
November 2, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29093508/vp2-of-chicken-anaemia-virus-interacts-with-apoptin-for-down-regulation-of-apoptosis-through-de-phosphorylated-threonine-108-on-apoptin
#12
Guan-Hua Lai, Yi-Yang Lien, Ming-Kuem Lin, Jai-Hong Cheng, Jason Tc Tzen, Fang-Chun Sun, Meng-Shiunn Lee, Hsi-Jien Chen, Meng-Shiou Lee
Chicken anaemia virus (CAV) is an important contagious agent that causes immunosuppressive disease in chickens. CAV Apoptin is a nucleoplasmic shuffling protein that induces apoptosis in chicken lymphoblastoid cells. In the present study, confocal microscopy revealed co-localisation of expressed CAV non-structural protein VP2 with Apoptin in the nucleus of MDCC-MSB1 cells and the nucleoplasmic compartment of CHO-K1 cells. In vitro pull-down and ex vivo biomolecular fluorescent complementation (BiFC) assays further showed that the VP2 protein directly interacts with Apoptin...
November 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29090854/optimized-selection-marker-and-cho-host-cell-combinations-for-generating-high-monoclonal-antibody-producing-cell-lines
#13
Jessna H M Yeo, Steven C L Ho, Mariati Mariati, Esther Koh, Shi Jie Tay, Susanto Woen, Peiqing Zhang, Yuansheng Yang
There are several selection markers which are suitable for generating stably transfected Chinese hamster ovary (CHO) cell lines. Due to their different modes of action, each selection marker has its own optimal selection stringency in different host cells for obtaining high productivity. Using an internal ribosome entry site (IRES)-mediated tricistronic vector and a set of IRES variants with different strengths, the expression of five antibiotics resistance genes (ARGs) in CHO-K1 cells and dihydrofolate reductase (DHFR) in CHO DG44 cells is optimized to enhance the stringency of selection for high producing cells...
September 25, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29090488/validation-of-keratometric-measurements-obtained-with-an-intraoperative-image-guided-system-intra-session-repeatability-and-interchangeability-with-an-optical-biometer
#14
Clara Ruiz-Belda, Fermín Rodrigo, David P Piñero
PURPOSE: To evaluate the intra-session repeatability of keratometric measurements obtained in healthy eyes with the Verion image-guided system (Alcon Laboratories Inc, Fort Worth, Texas, USA) as well as the interchangeability of such measurements with those obtained with an optical biometer (Aladdin, Topcon, Tokyo, Japan). METHODS: A total of 53 eyes of 53 patients (age 31-67 years) were enrolled in the study. All eyes received a comprehensive ophthalmologic examination including an analysis with the Verion image-guided and Aladdin systems...
October 31, 2017: Clinical & Experimental Optometry: Journal of the Australian Optometrical Association
https://www.readbyqxmd.com/read/29090231/cloning-and-expression-of-recombinant-human-interleukin-7-in-chinese-hamster-ovary-cho-cells
#15
Fatemeh Sadat Toghraie, Seyedeh Masoumeh Sharifzadeh, Amin Ramezani, Elham Mahmoudi Maymand, Mahsa Yazdanpanah-Samani, Abbas Ghaderi
BACKGROUND: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. METHODS: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected with both circular and linear forms of the pBud-hIL-7 recombinant by electroporation...
October 2017: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29084156/the-dietary-furocoumarin-imperatorin-increases-plasma-glp-1-levels-in-type-1-like-diabetic-rats
#16
Lin-Yu Wang, Kai-Chun Cheng, Yingxiao Li, Chiang-Shan Niu, Juei-Tang Cheng, Ho-Shan Niu
Imperatorin, a dietary furocoumarin, is found not only in medicinal plants, but also in popular culinary herbs, such as parsley and fennel. Recently, imperatorin has been shown to activate GPR119 in cells. Another GPR, GPR131, also called TGR5 or G-protein-coupled bile acid receptor 1 (GPBAR1), is known to regulate glucose metabolism. Additionally, TGR5 activation increases glucagon-like peptide (GLP-1) secretion to lower blood sugar levels in animals. Therefore, the present study aims to determine whether the effects of imperatorin on GLP-1 secretion are mediated by TGR5...
October 30, 2017: Nutrients
https://www.readbyqxmd.com/read/29081866/conventional-versus-accelerated-collagen-cross-linking-for-keratoconus-a-comparison-of-visual-refractive-topographic-and-biomechanical-outcomes
#17
Jyh Haur Woo, Jayant Venkatramani Iyer, Li Lim, M Htoon Hla, Jodhbir S Mehta, Cordelia Ml Chan, Donald Th Tan
OBJECTIVE: The aim was to compare the visual, refractive, topographic and biomechanical outcomes in patients with progressive keratoconus treated with either conventional or accelerated crosslinking at one year follow up. METHODS: It is a prospective, non-randomised interventional study of 76 patients who underwent conventional (CXL; 3mW/cm(2) for 30 minutes) or accelerated cross linking (KXL; 30mW/cm(2) for 4 minutes) for progressive keratoconus. Baseline and postoperative visual acuity, manifest refraction, corneal topography, pachymetry, endothelial cell density and biomechanical parameters of corneal hysteresis and corneal resistance factor were evaluated and compared...
2017: Open Ophthalmology Journal
https://www.readbyqxmd.com/read/29076990/expression-of-k1-toxin-derivatives-in-saccharomyces-cerevisiae-mimics-treatment-with-exogenous-toxin-and-provides-a-useful-tool-for-elucidating-k1-mechanisms-of-action-and-immunity
#18
Stefanie Gier, Manfred J Schmitt, Frank Breinig
Killer toxin K1 is a heterodimeric protein toxin secreted by Saccharomyces cerevisiae strains infected with the M1 double-stranded RNA 'killer' virus. After binding to a primary receptor at the level of the cell wall, K1 interacts with its secondary plasma membrane receptor Kre1p, eventually leading to an ionophoric disruption of membrane function. Although it has been under investigation for decades, neither the particular mechanisms leading to toxicity nor those leading to immunity have been elucidated. In this study, we constructed derivatives of the K1α subunit and expressed them in sensitive yeast cells...
October 27, 2017: Toxins
https://www.readbyqxmd.com/read/29071236/performance-and-specificity-of-6-immunoassays-for-tsh-receptor-antibodies-a-multicenter-study
#19
Tanja Diana, Christian Wüster, Paul D Olivo, Angelica Unterrainer, Jochem König, Michael Kanitz, Artur Bossowski, Brigitte Decallonne, George J Kahaly
BACKGROUND: The measurement of TSH receptor (TSHR) antibodies is warranted for diagnosis of Graves' disease (GD). OBJECTIVE: The performance, detection sensitivity, and specificity of 6 TSHR immunoassays were compared. METHODS: Two bioassays and 4 binding assays (Kronus, Immulite, Kryptor, Dynex) were compared in a dilution study performed in patients with autoimmune thyroid disease. Both bioassays were compared to 2 binding assays using stimulatory (M22) and blocking (K1-70) monoclonal antibody (MAb) mixtures...
September 2017: European Thyroid Journal
https://www.readbyqxmd.com/read/29066548/genome-wide-discovery-of-genes-required-for-capsule-production-by-uropathogenic-escherichia-coli
#20
Kelvin G K Goh, Minh-Duy Phan, Brian M Forde, Teik Min Chong, Wai-Fong Yin, Kok-Gan Chan, Glen C Ulett, Matthew J Sweet, Scott A Beatson, Mark A Schembri
Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract and bloodstream infections and possesses an array of virulence factors for colonization, survival, and persistence. One such factor is the polysaccharide K capsule. Among the different K capsule types, the K1 serotype is strongly associated with UPEC infection. In this study, we completely sequenced the K1 UPEC urosepsis strain PA45B and employed a novel combination of a lytic K1 capsule-specific phage, saturated Tn5 transposon mutagenesis, and high-throughput transposon-directed insertion site sequencing (TraDIS) to identify the complement of genes required for capsule production...
October 24, 2017: MBio
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