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https://www.readbyqxmd.com/read/29333616/determination-of-vitamins-k1-mk-4-and-mk-7-in-human-serum-of-postmenopausal-women-by-hplc-with-fluorescence-detection
#1
Eva Klapkova, Jana Cepova, Katerina Dunovska, Richard Prusa
BACKGROUND: New high-performance liquid chromatography (HPLC) method was developed for the determination of vitamin K1 and two forms of vitamin K2 (MK-4 and MK-7) in human serum, and the levels of vitamin K were determined in 350 samples of postmenopausal women. METHODS: Vitamin K was determined by HPLC with fluorescence detection after postcolumn zinc reduction. The detection was performed at 246 nm (excitation) and 430 nm (emission). The internal standard and 2 mL of ethanol were added to 500 μL of serum...
January 15, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29331715/toxicokinetics-of-zn-and-cd-in-the-earthworm-eisenia-andrei-exposed-to-metal-contaminated-soils-under-different-combinations-of-air-temperature-and-soil-moisture-content
#2
M Nazaret González-Alcaraz, Susana Loureiro, Cornelis A M van Gestel
This study evaluated how different combinations of air temperature (20 °C and 25 °C) and soil moisture content (50% and 30% of the soil water holding capacity, WHC), reflecting realistic climate change scenarios, affect the bioaccumulation kinetics of Zn and Cd in the earthworm Eisenia andrei. Earthworms were exposed for 21 d to two metal-contaminated soils (uptake phase), followed by 21 d incubation in non-contaminated soil (elimination phase). Body Zn and Cd concentrations were checked in time and metal uptake (k1) and elimination (k2) rate constants determined; metal bioaccumulation factor (BAF) was calculated as k1/k2...
January 8, 2018: Chemosphere
https://www.readbyqxmd.com/read/29329781/dopamine-transporter-trafficking-is-regulated-by-neutral-sphingomyelinase-2-ceramide-kinase
#3
Jong Hoon Won, Seok Kyun Kim, In Chul Shin, Hae Chan Ha, Ji Min Jang, Moon Jung Back, Dae Kyong Kim
Dopamine (DA) reuptake is the primary mechanism to terminate dopaminergic transmission in the synaptic cleft. The dopamine transporter (DAT) has an important role in the regulation of DA reuptake. This study provides anatomical and physiological evidence that DAT recycling is regulated by ceramide kinase via the sphingomyelin pathway. First, the results show that DAT and neutral sphingomyelinase 2 (nSMase2) were successfully co-precipitated from striatal samples and were colocalized in the mouse striatum or PC12 cells...
January 9, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29328525/a-role-of-sialic-acid-containing-glycans-of-matrix-metalloproteinase-9-mmp-9-in-the-interaction-between-mmp-9-and-staphylococcal-superantigen-like-protein-5-ssl5
#4
Chisato Kurisaka, Teruaki Oku, Saotomo Itoh, Tsutomu Tsuji
The staphylococcal superantigen-like proteins (SSLs) exhibit no superantigenic activity but have recently been considered to act as immune suppressors. It was previously reported that SSL5 bound to P-selectin glycoprotein ligand-1 (PSGL-1) and matrix metalloproteinase (MMP)-9, leading to inhibition of leukocyte adhesion and invasion. These interactions were suggested to depend on sialic acid-containing glycans of MMP-9, but the roles of sialic acids in the interaction between SSL5 and MMP-9 are still controversial...
January 12, 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/29327295/common-deficiencies-of-in-vitro-binding-bioequivalence-be-studies-submitted-in-abbreviated-new-drug-applications-andas
#5
Dongmei Lu, Diana Vivian, Ping Ren, Yongsheng Yang, Hongling Zhang, Xiaojian Jiang, Ethan Stier
There are several drug products that bind phosphate or bile acid in the gastrointestinal (GI) tract to exert their therapeutic efficacy. In vitro binding studies are used to assess bioequivalence (BE) of these products. The objective of this study is to identify the common deficiencies in Abbreviated New Drug Applications (ANDAs) for these products. Deficiencies were compiled from ANDAs containing in vitro binding BE studies. The deficiencies were classified into eight categories: Pre-Study Method Validation, During-Study Sample Analysis, Study Design, Study Procedure, Dissolution/Disintegration, Analytical Site Inspection, Data Submission, and Formulations...
January 11, 2018: AAPS Journal
https://www.readbyqxmd.com/read/29326806/negative-impact-of-prolonged-antibiotics-or-persistent-diarrhea-on-vitamin-k1-levels-in-2-24-weeks-aged-egyptian-infants
#6
Mohsen S Elalfy, Fatma S E Ebeid, Iman A Elagouza, Fatma A Ibrahim, Noura B M Hassan, Beshoy A Botros
Background: To evaluate the hazard of prolonged antibiotic therapy and/or persistent diarrhea on vitamin K1 (VK1) level and bleeding profile in infants (2-24 weeks). Methods: A one-year case-control study, conducted at Ain Shams University, Egypt. 338 infants (2-24 weeks) were recruited and divided into 3 groups (1:1:3 ratios); group A (n=67) patients who received antibiotics for ≥10 days, group B (n=67) who had persistent diarrhea ≥ 14 days and group C (n=204) age- and gender- matched infants who had not either received antibiotics nor had diarrhea...
2018: Mediterranean Journal of Hematology and Infectious Diseases
https://www.readbyqxmd.com/read/29324813/increase-in-extraction-of-i-123-iomazenil-in-patients-with-chronic-cerebral-ischemia
#7
Hiroki Kato, Kayako Isohashi, Eku Shimosegawa, Jun Hatazawa
BACKGROUND: Cerebral extraction of diffusively distributed substances like oxygen has been suggested to change according to the cerebral blood flow (CBF) and status of the microvasculature. The relationships between the cerebral extraction of diffusively distributed lipophilic tracers and the severity of cerebral ischemia has not yet been clarified. In the present study, we attempted to elucidate the association between the extraction fraction of the lipophilic tracer I-123 iomazenil (IMZ) (IMZ-EF) and the oxygen extraction fraction (OEF) derived from O-15 PET in patients with chronic steno-occlusive disease of internal carotid artery (ICA) or middle cerebral artery (MCA)...
2018: PloS One
https://www.readbyqxmd.com/read/29324704/dissection-of-myogenic-differentiation-signatures-in-chickens-by-rna-seq-analysis
#8
Tingting Li, Genxi Zhang, Pengfei Wu, Lian Duan, Guohui Li, Qiuhong Liu, Jinyu Wang
A series of elaborately regulated and orchestrated changes in gene expression profiles leads to muscle growth and development. In this study, RNA sequencing was used to profile embryonic chicken myoblasts and fused myotube transcriptomes, long non-coding RNAs (lncRNAs), and messenger RNAs (mRNAs) at four stages of myoblast differentiation. Of a total of 2484 lncRNA transcripts, 2288 were long intergenic non-coding RNAs (lincRNAs) and 198 were antisense lncRNAs. Additionally, 1530 lncRNAs were neighboring 2041 protein-coding genes (<10 kb upstream and downstream) and functionally enriched in several pathways related to skeletal muscle development that have been extensively studied, indicating that these genes may be in cis-regulatory relationships...
January 11, 2018: Genes
https://www.readbyqxmd.com/read/29319752/effectiveness-of-aqueous-and-hydroalcoholic-extracts-of-acanthospermum-australe-loefl-kuntze-against-diarrhea-inducing-bacteria
#9
R Mallmann, E M Ethur, P Bianchetti, D Faleiro, L Hoehne, M I Goettert
Leaves and roots of Acanthospermum australe (Asteraceae) have been used in Brazilian folk medicine for the treatment of various ailments including diarrhea, skin diseases, blennorrhagia, dyspepsia, parasitic worms and malaria. The aim of study was to characterize the chemical profiles of the aqueous and hydroalcoholic extracts of leaves and roots of A. australe, and to evaluate their antimicrobial activities against diarrhea-inducing bacteria (Enterococcus faecalis, Shigella dysenteriae and Yersinia enterocolitica), as well as their cytotoxic properties...
January 8, 2018: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/29319515/the-determination-and-correlation-of-various-virulence-genes-esbl-serum-bactericidal-effect-and-biofilm-formation-of-clinical-isolated-classical-klebsiella-pneumoniae-and-hypervirulent-klebsiella-pneumoniae-from-respiratory-tract-infected-patients
#10
Rambha K Shah, Zhao H Ni, Xiao Y Sun, Guo Q Wang, Fan Li
Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of 96 K. pneumoniae strains were isolated from sputum of respiratory tract infected patients...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29317166/synthesis-in-vitro-antimalarial-activities-and-cytotoxicities-of-amino-artemisinin-ferrocene-derivatives
#11
Christo de Lange, Dina Coertzen, Frans J Smit, Johannes F Wentzel, Ho Ning Wong, Lyn-Marie Birkholtz, Richard K Haynes, David D N'Da
Novel derivatives bearing a ferrocene attached via a piperazine linker to C-10 of the artemisinin nucleus were prepared from dihydroartemisinin and screened against chloroquine (CQ) sensitive NF54 and CQ resistant K1 and W2 strains of Plasmodium falciparum (Pf) parasites. The overall aim is to imprint oxidant (from the artemisinin) and redox (from the ferrocene) activities. In a preliminary assessment, these compounds were shown to possess activities in the low nM range with the most active being compound 6 with IC50 values of 2...
December 26, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29315781/bioconcentration-model-for-non-ionic-polar-and-ionizable-organic-compounds-in-amphipod
#12
Ciara Chun Chen, Dave Ta Fu Kuo
This study presents a bioconcentration model for non-ionic, polar, and ionizable organic compounds in amphipod based on first-order kinetics. Uptake rate constant k1 is modeled as log k1=10.81logKOW+0.15 (root mean square error, RMSE = 0.52). Biotransformation rate constant kM is estimated using an existing polyparameter linear free energy relationship model. Respiratory elimination k2 is calculated as modeled k1 over theoretical biota-water partition coefficient Kbiow considering the contributions of lipid, protein, carbohydrate and water...
January 9, 2018: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29306077/the-e3-ubiquitin-ligase-hectd1-is-involved-in-abca1-mediated-cholesterol-export-from-macrophages
#13
Shereen M Aleidi, Alryel Yang, Laura J Sharpe, Geetha Rao, Blake J Cochran, Kerry-Anne Rye, Maaike Kockx, Andrew J Brown, Ingrid C Gelissen
The ABC lipid transporters, ABCA1 and ABCG1, are essential for maintaining lipid homeostasis in cells such as macrophages by exporting excess cholesterol to extracellular acceptors. These transporters are highly regulated at the post-translational level, including protein ubiquitination. Our aim was to investigate the role of the E3 ubiquitin ligase HECTD1, recently identified as associated with ABCG1, on ABCG1 and ABCA1 protein levels and cholesterol export function. Here, we show that HECTD1 protein is widely expressed in a range of human and murine primary cells and cell lines, including macrophages, neuronal cells and insulin secreting β-cells...
January 3, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29304283/kinetics-and-products-of-the-reaction-of-oh-radicals-with-clno-from-220-to-940k
#14
Yuri Bedjanian
The kinetics and products of the reaction of OH radicals with ClNO have been studied in a flow reactor coupled with an electron impact ionization mass spectrometer at nearly 2 Torr total pressure of helium and over a wide temperature range, T = 220 - 940 K. The rate constant of the reaction OH + ClNO  products (1) was determined under pseudo-first order conditions, monitoring the kinetics of OH consumption in excess of ClNO: k1 = 1.48 × 10-18 × T2.12 exp(146/T) cm3molecule-1s-1 (uncertainty of 15%). HOCl, Cl and HONO were observed as the reaction products...
January 5, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29299168/lncrna-crnde-promotes-cell-proliferation-invasion-and-migration-by-competitively-binding-mir-384-in-papillary-thyroid-cancer
#15
Honggang Sun, Liqin He, Lan Ma, Tao Lu, Jianguo Wei, Kejie Xie, Xingmu Wang
Thyroid cancer is one of the most prevalent endocrine neoplasm. The present study examined the effects of Colorectal Neoplasia Differentially Expressed (CRNDE) on the progression of papillary thyroid cancer (PTC), and explored the underlying molecular mechanisms. Quantitative real-time PCR was used to detect CRNDE, miR-384 and pleiotrophin (PTN) mRNA expression. Western blot was used to measure PTN protein levels. Cell proliferation, cell growth, cell invasion and migration of PTC cells were determined by CCK-8, colony formation, transwell invasion and migration assays, respectively...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29291530/cellular-biomechanics-impairment-in-keratinocytes-is-associated-with-a-c-terminal-truncated-desmoplakin-an-atomic-force-microscopy-investigation
#16
Luca Puzzi, Daniele Borin, Valentina Martinelli, Luisa Mestroni, David P Kelsell, Orfeo Sbaizero
In a tissue continuously challenged by mechanical stresses, such as the skin or the heart, cells perceive information about their microenvironment through several adhesive protein complexes and activate cell-signaling events to maintain tissue cohesion. Consequently, alteration of cell adhesion components leads to aberrant assembly of the associated cytoplasmic scaffolding and signaling pathways, which may reflect changes to the tissue physiology and mechanical resistance. Desmoplakin is an essential component of the cell-cell junction, anchoring the desmosomal protein complex to the intermediate filaments (IFs)...
December 19, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29290400/evaluation-of-reversed-phase-nano-liquid-chromatography-conditions-by-using-reversed-phase-thin-layer-chromatography-based-on-hansen-solubility-parameters-for-the-analysis-of-amphiphilic-glycosylsphingolipid-transformations
#17
Yoshimi Kanie, Mizuki Taniuchi, Osamu Kanie
Pulse chase analysis is often used in investigating dynamics of cellular substances. Fluorescently labeled lactosyl sphingosine molecule is useful in chasing its transformation, however the analysis of such metabolites in attomole level is of extreme difficult due to the presence of large amount of endogenous amphiphilic molecules such as glycosphingolipids, sphingomyerin, and glycerophospholipids. Nano LC suites for analyzing the attomole scale metabolites, therefore removal of endogenous substances prior to nano LC and finding appropriate nano LC conditions are necessary...
December 23, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29281044/radiobiological-response-of-u251mg-cho-k1-and-v79-cell-lines-to-accelerator-based-boron-neutron-capture-therapy
#18
Eisuke Sato, Alexander Zaboronok, Tetsuya Yamamoto, Kei Nakai, Sergey Taskaev, Olga Volkova, Ludmila Mechetina, Alexander Taranin, Vladimir Kanygin, Tomonori Isobe, Bryan J Mathis, Akira Matsumura
In the current article, we provide in vitro efficacy evaluation of a unique accelerator-based neutron source, constructed at the Budker Institute of Nuclear Physics (Novosibirsk, Russian Federation), for boron neutron capture therapy (BNCT), which is particularly effective in the case of invasive cancers. U251MG, CHO-K1 and V79 cells were incubated and irradiated in various concentrations of boric acid with epithermal neutrons for 2-3 h in a plexiglass phantom, using 2.0 MeV proton energy and 1.5-3.0 mA proton current, resulting in a neutron fluence of 2...
December 21, 2017: Journal of Radiation Research
https://www.readbyqxmd.com/read/29276973/molecular-and-functional-characterization-of-tumor-induced-factor-tif-hamster-homolog-of-cxcl3-gro%C3%AE-displays-tumor-suppressive-activity
#19
Lili Jin, Zhou-Fang Li, Da-Kui Wang, Meina Sun, Wei Qi, Qiang Ma, Li Zhang, Chun Chu, Elaine Y M Chan, Susanna S T Lee, Helen Wise, Ka-Fai To, Ying Shi, Naiming Zhou, Wing-Tai Cheung
Previously our lab has created a mouse ovarian xenograft model of copy number variation (CNV)-mediated G protein-coupled receptor (GPCR) MAS-driven tumorigenesis, and RNA profiling identified a putative chemokine tumor-induced factor (Tif). Sequence analysis and chemotactic study suggested that Tif was likely to be a hamster homolog of human GROγ (CXCL3) [IJC 125 (2009): 1316-1327]. In the present study, we report the molecular and functional characterization of the Tif gene. Genomic study of CHO-K1 cells indicated that Tif gene consisted of 4 exons, characterized with an antisense B1 element which is embedded in the fourth exon...
December 22, 2017: Cytokine
https://www.readbyqxmd.com/read/29275478/degradation-reaction-of-diazo-reactive-black-5-dye-with-copper-ii-sulfate-catalyst-in-thermolysis-treatment
#20
Yen-Yie Lau, Yee-Shian Wong, Tze-Zhang Ang, Soon-An Ong, Nabilah Aminah Lutpi, Li-Ngee Ho
The theme of present research demonstrates performance of copper (II) sulfate (CuSO4) as catalyst in thermolysis process to treat reactive black 5 (RB 5) dye. During thermolysis without presence of catalyst, heat was converted to thermal energy to break the enthalpy of chemical structure bonding and only 31.62% of color removal. With CuSO4 support as auxiliary agent, the thermally cleaved molecular structure was further destabilized and reacted with CuSO4. Copper ions functioned to delocalize the coordination of π of the lone paired electron in azo bond, C=C bond of the sp2 carbon to form C-C of the sp3 amorphous carbon in benzene and naphthalene...
December 23, 2017: Environmental Science and Pollution Research International
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