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https://www.readbyqxmd.com/read/28225793/vimentin-regulates-differentiation-switch-via-modulation-of-keratin-14-levels-and-their-expression-together-correlates-with-poor-prognosis-in-oral-cancer-patients
#1
Crismita Dmello, Sharada Sawant, Hunain Alam, Prakash Gangadaran, Saie Mogre, Richa Tiwari, Zinia D'Souza, Manish Narkar, Rahul Thorat, Komal Patil, Devendra Chaukar, Shubhada Kane, Milind Vaidya
Vimentin is an intermediate filament protein, predominantly expressed in cells of mesenchymal origin, although its aberrant expression is seen in many carcinomas during epithelial mesenchymal transition. In cancer, vimentin expression is associated with the transition from a more differentiated epithelial phenotype to a dedifferentiated state. In view of the perceived role of keratins (Ks) as regulators of differentiation in epithelia, it was important to understand whether vimentin modulates differentiation through the reprogramming of keratins, in transformed cells...
2017: PloS One
https://www.readbyqxmd.com/read/28224115/the-escherichia-coli-serogroup-o1-and-o2-lipopolysaccharides-are-encoded-by-multiple-o-antigen-gene-clusters
#2
Sabine Delannoy, Lothar Beutin, Patricia Mariani-Kurkdjian, Aubin Fleiss, Stéphane Bonacorsi, Patrick Fach
Escherichia coli strains belonging to serogroups O1 and O2 are frequently associated with human infections, especially extra-intestinal infections such as bloodstream infections or urinary tract infections. These strains can be associated with a large array of flagellar antigens. Because of their frequency and clinical importance, a reliable detection of E. coli O1 and O2 strains and also the frequently associated K1 capsule is important for diagnosis and source attribution of E. coli infections in humans and animals...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28222865/simulated-oil-release-from-oil-contaminated-marine-sediment-in-the-bohai-sea-china
#3
Lingling Yuan, Longxi Han, Wenjie Bo, Hua Chen, Wenshen Gao, Bo Chen
There is a high degree of heavy oil partitioning into marine sediments when an oil spill occurs. Contaminated sediment, as an endogenous pollution source, can re-pollute overlying water slowly. In this study, a static oil release process and its effects in marine sediment was investigated through a series of experiments with reproductive heavy oil-contaminated marine sediment. The oil release process was accurately simulated with a Lagergren first-order equation and reached equilibration after 48h. The fitted curve for equilibrium concentration (C0) and first-order rate constant (k1) for sediment pollution levels exhibited a first-order log relationship...
February 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28220270/multiplex-assessment-of-serum-cytokine-and-chemokine-levels-in-idiopathic-morphea-and-vitamin-k1-induced-morphea
#4
Lori Ann Cox, Guy F Webster, Sonsoles Piera-Velazquez, Sergio A Jimenez
The levels of 63 cytokines, chemokines, and growth factors were measured in the serum of four patients with idiopathic morphea and of one patient with vitamin K1-induced morphea employing a multiplex assay to identify the role of inflammatory/immunologic events in their pathogenesis. Full-thickness skin biopsies of affected skin were analyzed by histopathology. Luminex assays for 63 cytokines, chemokines, and growth factors were performed in the sera from four patients with idiopathic morphea and in two different samples of serum obtained in two separate occasions from one patient with vitamin K1-induced morphea...
February 20, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28217351/k1-2x-ni1-x-fe2-aso4-3-x-0-125-un-nouvel-ars%C3%A3-niate-%C3%A3-structure-de-type-%C3%AE-crpo4
#5
Ridha Ben Smail, Mohamed Faouzi Zid
A new arsenate K1+2x Ni1-x Fe2(AsO4)3 (x = 1/8) {potassium nickel diiron(III) tris-[arsenate(V)]} was synthesized using a flux method and its crystal structure was determined from single-crystal X-ray diffraction data. This material was also characterized by qualitative energy dispersive X-ray spectroscopy (EDS) analysis. The crystal structure belongs to the α-CrPO4-structure type, space group Imma. It consists of a three-dimensional-framework built up from FeO6 and Ni0.875□1.25O6-octa-hedra and AsO4-tetra-hedra that are sharing corners and/or edges, generating tunnels running along the [010] and [001] directions in which the potassium cations are located...
February 1, 2017: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/28216629/matrix-attachment-region-combinations-increase-transgene-expression-in-transfected-chinese-hamster-ovary-cells
#6
Chun-Peng Zhao, Xiao Guo, Si-Jia Chen, Chang-Zheng Li, Yun Yang, Jun-He Zhang, Shao-Nan Chen, Yan-Long Jia, Tian-Yun Wang
Matrix attachment regions (MARs) are cis-acting DNA elements that can increase transgene expression levels in a CHO cell expression system. To investigate the effects of MAR combinations on transgene expression and the underlying regulatory mechanisms, we generated constructs in which the enhanced green fluorescent protein (eGFP) gene flanked by different combinations of human β-interferon and β-globin MAR (iMAR and gMAR, respectively), which was driven by the cytomegalovirus (CMV) or simian virus (SV) 40 promoter...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28211929/calcium-phosphate-nanoparticles-prepared-from-infusion-fluids-for-stem-cell-transfection-process-optimization-and-cytotoxicity-analysis
#7
Quazi T H Shubhra, Ayako Oyane, Hiroko Araki, Maki Nakamura, Hideo Tsurushima
This is the first study to report the use of infusion fluids for particle-mediated gene delivery with DNA-immobilized calcium phosphate (CaP) nanoparticles (NPs). In conventional CaP systems, CaP NPs are fabricated in labile supersaturated CaP solutions which are prepared from chemical reagents. In the present study, we fabricated CaP NPs via coprecipitation in labile supersaturated CaP solutions that were prepared from infusion fluids (even the water used was of injectable quality) instead of chemical reagents and demonstrated their gene delivery capabilities for the hard to transfect pluripotent stem cell (C3H10T1/2) along with the easy to transfect CHO-K1 cell...
February 17, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28203549/microbiological-and-clinical-characteristics-of-hypermucoviscous-klebsiella-pneumoniae-isolates-associated-with-invasive-infections-in-china
#8
Yinjuan Guo, Shanshan Wang, Lingling Zhan, Ye Jin, Jingjing Duan, Zhihao Hao, Jingnan Lv, Xiuqin Qi, Liang Chen, Barry N Kreiswirth, Liangxing Wang, Fangyou Yu
A distinctive syndrome caused by hypermucoviscous Klebsiella pneumoniae (HMKP) including pyogenic liver abscess (PLA) is now becoming a globally emerging disease. In the present study, 22.8% (84/369) of K. pneumoniae clinical isolates associated with various types of invasive infections were identified as HMKP, with 45.2% associated with PLA. Multivariate regression analysis showed that male patients with 41-50 years, PLA, diabetes mellitus, and hypertension were independent risk factors for HMKP infections...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28197787/dermatux-phase-iv-trial-of-cetuximab-plus-folfiri-in-first-line-metastatic-colorectal-cancer-receiving-a-pre-defined-skin-care
#9
Carl Christoph Schimanski, Frank Staib, Thomas Göhler, Holger Hebart, Michael Heike, Michael Neise, Jochen Rudi, Thomas Geer, Gerrit Dingeldein, Claudia Lang, Peter Ehscheidt, Thomas Flohr, Klaus Maria Josten, Meinolf Karthaus, Alexander Schmittel, Jan Wierecky, Emil Boller, Martin Indorf, Marcus-Alexander Wörns, Peter R Galle, Markus Moehler
PURPOSE: Cetuximab-induced skin rash Gd3+ occurs in ≥16% patients (pts) (Heinemann et al., Lancet Oncol 15(10):1065-1075, 2014; Van Cutsem et al. J Clin Oncol 27(19):3117-25; 2009b). Survival, response, and toxicity parameters were re-evaluated under a pre-defined skin prophylaxis consistent of vitamin K1 ointment and oral doxycycline. METHODS: This is a national, multicenter, phase 4, first-line mCRC (K-RAS wt) trial. Pts received irinotecan 180 mg/m² (d1), FA 400 mg/m² (d1), 5-FU 400 mg/m² (d1), 5-FU 2400 mg/m² (d1-2), and cetuximab [400 mg/m² (d1), and then 250 mg/m² qw], prophylactic 0...
February 14, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28196047/a-time-dependent-model-describes-methotrexate-elimination-and-supports-dynamic-modification-of-mrp2-abcc2-activity
#10
Jean-Baptiste Woillard, Jean Debord, Isabelle Benz-de-Bretagne, Franck Saint-Marcoux, Pascal Turlure, Stéphane Girault, Julie Abraham, Sylvain Choquet, Pierre Marquet, Chantal Barin-Le Guellec
BACKGROUND: MRP2/ABCC2, an efflux transporter expressed at the proximal renal tubule, is rate limiting for urine excretion of coproporphyrin (UCP) isomers I and III, translating in high UCP (I/(I+III)) ratio in MRP2-deficient patients presenting with the Dubin-Johnson Syndrome. MRP2 is also a major contributor to methotrexate (MTX) clearance. As MTX is both a substrate and an inhibitor of MRP2, time course of the concentrations of MTX in blood could induce functional modification of MRP2 over time, which in turn can modify its own elimination rate...
February 11, 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28192590/tomographic-findings-after-implantation-of-ferrara-intrastromal-corneal-ring-segments-in-keratoconus
#11
João Marcelo Lyra, Daniela Lyra, Guilherme Ribeiro, Leonardo Torquetti, Paulo Ferrara, Aydano Machado
PURPOSE: To analyze and compare changes to the anterior and posterior corneal surfaces after Ferrara intracorneal ring segment (ICRS) (AJL, Boecillo, Spain) implantation and to correlate those changes with visual outcomes. METHODS: This retrospective case series study comprised consecutive patients with keratoconus implanted with the Ferrara ICRS. Computed tomography scans of the two corneal surfaces were obtained preoperatively and postoperatively with a rotating Scheimpflug imaging system...
February 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28183993/multiparametric-imaging-of-tumor-hypoxia-and-perfusion-with-18-f-fmiso-dynamic-pet-in-head-and-neck-cancer
#12
Milan Grkovski, Heiko Schöder, Nancy Lee, Sean D Carlin, Bradley Jay Beattie, Nadeem Riaz, Jonathan E Leeman, Joseph A O'Donoghue, John Laurence Humm
Tumor hypoxia and perfusion are independent prognostic indicators of patient outcome. We developed the methodology for, and investigated the utility of multiparametric imaging of tumor hypoxia and perfusion with (18)F-fluoromisonidazole (FMISO) dynamic positron emission tomography (dPET) in head and neck cancer (HNC). Methods: 120 HNC patients underwent 0-30 min FMISO dPET in a customized immobilization mask, followed by 10 min static acquisitions starting at 93±6 and 160±13 min post-injection. A total of 248 lesions (≥2 cm(3)) were analyzed...
February 9, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28183666/fabrication-of-electrospun-hpgl-scaffolds-via-glycidyl-methacrylate-cross-linker-morphology-mechanical-and-biological-properties
#13
Fernando José Costa Baratéla, Olga Zazuco Higa, Esdras Duarte Dos Passos, Alvaro Antonio Alencar de Queiroz
Electrospinning is a suitable method to produce scaffolds composed of nanoscale to microscale fibers, which are comparable to the extracellular matrix (ECM). Hyperbranched polyglycerol (HPGL) is a highly biocompatible polyether polyol potentially useful for the design of fibrous scaffolds mimicking the ECM architecture. However, scaffolds developed from HPGL have poor mechanical properties and morphological stability in the aqueous environments required for tissue engineering applications. This work reports the production of stable electrospun HPGL scaffolds (EHPGLS) using glycidyl methacrylate (GMA) as cross-linker to enhance the water stability and mechanical property of electrospun HPGL...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28183058/in%C3%A2-vitro-and-in%C3%A2-vivo-investigation-of-the-genotoxic-potential-of-waters-from-rivers-under-the-influence-of-a-petroleum-refinery-s%C3%A3-o-paulo-state-brazil
#14
Raquel Vaz Hara, Maria Aparecida Marin-Morales
In recent years concern about the chemical composition of wastewater generated by the oil refining industry has increased, even after its treatment. These wastewaters contain substances that can harm both the entire aquatic ecosystem and the health of any exposed organisms. The aim of this study was to evaluate the genotoxic and mutagenic potentials of the effluent generated by the largest Brazilian petroleum refinery, the effectiveness of the treatments used by the refinery, and whether its effluent can compromise the water quality of the river where it is discarded...
January 31, 2017: Chemosphere
https://www.readbyqxmd.com/read/28171837/efficient-photoreductive-decomposition-of-n-nitrosodimethylamine-by-uv-iodide-process
#15
Zhuyu Sun, Chaojie Zhang, Xiaoyun Zhao, Jing Chen, Qi Zhou
N-nitrosodimethylamine (NDMA) has aroused extensive concern as a disinfection byproduct due to its high toxicity and elevated concentration levels in water sources. This study investigates the photoreductive decomposition of NDMA by UV/iodide process. The results showed that this process is an effective strategy for the treatment of NDMA with 99.2% NDMA removed within 10min. The depletion of NDMA by UV/iodide process obeyed pseudo-first-order kinetics with a rate constant (k1) of 0.60±0.03min(-1). Hydrated electrons (eaq(-)) generated by the UV irradiation of iodide were proven to play a critical role...
December 26, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28168930/avirulence-avr-gene-based-diagnosis-complements-existing-pathogen-surveillance-tools-for-effective-deployment-of-resistance-r-genes-against-rice-blast-disease
#16
Shiela Marie Gines Selisana, Mary Jeanie Yanoria, Berlaine Quime, Chaivarakun Chaipanya, Guodong Lu, Rina Bagsic Opulencia, Guo-Liang Wang, Thomas Mitchell, James C Correll, Nick Talbot, Hei Leung, Bo Zhou
Avirulence genes in Magnaporthe oryzae, the fungal pathogen that causes the devastating rice blast disease, have been documented to be major targets subject to mutations to avoid recognition by resistance (R) genes. In this study, an avirulence (AVR) gene-based diagnosis tool for determining the virulence spectrum of a rice blast pathogen population was developed and validated. A set of 77 single-spore field isolates was subjected to pathotype analysis using differential lines each containing a single R gene and classified into 20 virulent pathotypes except for four isolates that lost pathogenicity...
February 7, 2017: Phytopathology
https://www.readbyqxmd.com/read/28168382/molecular-design-expression-and-evaluation-of-pasylated-human-recombinant-erythropoietin-with-enhanced-functional-properties
#17
Mohammad Hossein Hedayati, Dariush Norouzian, Mahdi Aminian, Shahram Teimourian, Reza Ahangari Cohan, Soroush Sardari, M Reza Khorramizadeh
Erythropoietin (EPO) is the principal hormone which, has somewhat short half-life involved in the differentiation and regulation of circulating red blood cells. The present study was carried out to evaluate the capability of a polyethylene glycol mimetic technology as a biological alternative to improve pharmaceutical properties of human recombinant EPO. In silico models of EPO fused to 200 amino acids of proline, alanine, and serine (PAS) were initially generated and assessed by molecular dynamic (MD) simulation...
February 6, 2017: Protein Journal
https://www.readbyqxmd.com/read/28167160/comparative-study-of-the-cytotoxicity-and-genotoxicity-of-kaurenoic-acid-and-its-semi-synthetic-derivatives-methoxy-kaurenoic-acid-and-kaurenol-in-cho-k1-cells
#18
Bruno Limonti Cano, Monique Rodrigues Moreira, Mirian Oliveira Goulart, Natália Dos Santos Gonçalves, Rodrigo Cassio Sola Veneziani, Jairo Kenupp Bastos, Sérgio Ricardo Ambrósio, Raquel Alves Dos Santos
The diterpene kaurenoic acid (KA) has vasorelaxant, antimicrobial, anti-tumoural and anti-leishmanial effects. Semi-synthetic derivatives were obtained to achieve more satisfactory responses. The assessment of genotoxicity is part of the toxicological evaluation of therapeutic compound candidates. The present study investigated the cytotoxicity and genotoxicity of KA and its semi-synthetic derivatives methoxy kaurenoic acid (MKA) and kaurenol (KRN) using the CHO-K1 cell line. The cytotoxicity evaluation demonstrated that treatments with 200 and 400 μM KA reduced cellular proliferation to 36...
February 3, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28166786/chemical-constituents-and-coagulation-activity-of-agastache-rugosa
#19
Pengran Cao, Pingyao Xie, Xuebiao Wang, Jinmei Wang, Jinfeng Wei, Wen-Yi Kang
BACKGROUND: In the Chinese traditional medicine, plant of Agastache rugosa (Fisch. & C.A. Mey.) Kuntze (A. rugosa) has been used to treat nausea, vomiting and dispel damp. However, currently, few reports about the chemical constituents, especially the non-volatile components of A. rugosa are available. METHODS: Through separation with various column chromatographies to elucidate the chemical constituents of A. rugosa, the biological activities of the major constituents were investigated...
February 6, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28137615/regulation-of-mir-29b-1-a-transcription-and-identification-of-target-mrnas-in-cho-k1-cells
#20
Penn Muluhngwi, Kirsten Richardson, Joshua Napier, Eric C Rouchka, Justin L Mott, Carolyn M Klinge
miR-29b and miR-29a transcript levels were reported to increase in exponentially growing CHO-K1 cells. Here, we examine the regulation of miR-29b-1/a in CHO-K1 cells. We observed that 4-hydroxytamoxifen (4-OHT) increased pri-miR-29b-1 and pri-miR-29a transcription in CHO-K1 cells by activating endogenous estrogen receptor α (ERα). DICER, an established, bona fide target of miR-29b-1/a, was shown to be regulated by 4-OHT in CHO-K1 cells. We showed that miR-29b-1 and miR-29a serve a repressive role in cell proliferation, migration, invasion, and colony formation in CHO-K1 cells...
January 28, 2017: Molecular and Cellular Endocrinology
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