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V protein of paramyxovirus

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https://www.readbyqxmd.com/read/29343567/parainfluenza-virus-infection-sensitizes-cancer-cells-to-dna-damaging-agents-implications-for-oncolytic-virus-therapy
#1
Candace R Fox, Griffith D Parks
We have previously shown that the Parainfluenza virus 5 (PIV5) mutant P/V-CPI- is restricted for spread in normal cells but not in cancer cells in vitro and is effective at reducing tumor burden in mouse model systems. Here we show that P/V-CPI- infection of human laryngeal cancer HEp-2 cells results in the majority of the cells dying, but unexpectedly, over time there is an emergence of a population of cells which survive as P/V-CPI- persistently infected (PI) cells. P/V-CPI- PI cells had elevated levels of basal caspase activation, and viability was highly dependent on activity of cellular inhibitors of apoptosis (IAPs) such as Survivin and XIAP...
January 17, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29325564/engineered-recombinant-protein-products-of-the-avian-paramyxovirus-type-1-nucleocapsid-and-phosphoprotein-genes-for-serological-diagnosis
#2
Na Zhao, Christian Grund, Martin Beer, Timm C Harder
BACKGROUND: Virulent Newcastle disease virus (NDV, avian Avulavirus-1, APMV-1) induces a highly contagious and lethal systemic disease in gallinaceous poultry. APMV-1 antibody detection is used for surveillance and to control vaccination, but is hampered by cross-reactivity to other subtypes of avian Avulaviruses. Data are lacking concerning the applicability of NDV V proteins as differential diagnostic marker to distinguish vaccinated from virus-infected birds (DIVA strategy). METHODS: Full length and C-terminally truncated nucleocapsid (NP) protein, and the unique C-terminal regions of the phospho- (P) and V proteins of the NDV LaSota strain were bacterially expressed as fusion proteins with the multimerization domain of the human C4 binding protein, and used as diagnostic antigens in indirect ELISA...
January 11, 2018: Virology Journal
https://www.readbyqxmd.com/read/29321677/recognition-by-host-nuclear-transport-proteins-drives-disorder-to-order-transition-in-hendra-virus-v
#3
Sarah C Atkinson, Michelle D Audsley, Kim G Lieu, Glenn A Marsh, David R Thomas, Steven M Heaton, Jason J Paxman, Kylie M Wagstaff, Ashley M Buckle, Gregory W Moseley, David A Jans, Natalie A Borg
Hendra virus (HeV) is a paramyxovirus that causes lethal disease in humans, for which no vaccine or antiviral agent is available. HeV V protein is central to pathogenesis through its ability to interact with cytoplasmic host proteins, playing key antiviral roles. Here we use immunoprecipitation, siRNA knockdown and confocal laser scanning microscopy to show that HeV V shuttles to and from the nucleus through specific host nuclear transporters. Spectroscopic and small angle X-ray scattering studies reveal HeV V undergoes a disorder-to-order transition upon binding to either importin α/β1 or exportin-1/Ran-GTP, dependent on the V N-terminus...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321315/paramyxovirus-v-proteins-interact-with-the-rig-i-trim25-regulatory-complex-and-inhibit-rig-i-signaling
#4
Maria T Sanchez-Aparicio, Leighland J Feinman, Adolfo García-Sastre, Megan L Shaw
Paramyxovirus V proteins are known antagonists of the RIG-I-like receptor (RLR)-mediated interferon induction pathway, interacting with and inhibiting the RLR MDA5. We report interactions between the Nipah virus V protein and both the RIG-I regulatory protein, TRIM25, and RIG-I. We also observed interactions between these host proteins and the V proteins of measles virus, Sendai virus and parainfluenza virus. These interactions are mediated by the conserved C-terminal domain of the V protein, which binds to the tandem CARDs of RIG-I (the region of TRIM25-ubiquitination) and to the SPRY domain of TRIM25, which mediates TRIM25 interaction with the RIG-I CARDs...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29237838/a-single-amino-acid-substitution-within-the-paramyxovirus-sendai-virus-nucleoprotein-is-a-critical-determinant-for-production-of-ifn-beta-inducing-copyback-type-defective-interfering-genomes
#5
Asuka Yoshida, Ryoko Kawabata, Tomoyuki Honda, Kouji Sakai, Yasushi Ami, Takemasa Sakaguchi, Takashi Irie
One of the first defenses against infecting pathogens is the innate immune system activated by cellular recognition of pathogen-associated molecular patterns (PAMPs). Although virus-derived RNA species, especially copyback (cb)-type defective-interfering (DI) genomes, have been shown to serve as real PAMPs, which strongly induce interferon (IFN)-beta during mononegavirus infection, the mechanisms underlying DI generation remain unclear. Here, for the first time, we identified a single amino acid substitution causing production of cbDI genomes by successful isolation of two distinct types of viral clones with cbDI-producing and cbDI-non-producing phenotypes, from the stock Sendai virus (SeV) strain Cantell, which is widely used in a number of studies on anti-viral innate immunity as a representative IFN-beta-inducing virus...
December 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29129019/genetic-characterization-of-bank-vole-virus-bavv-a-new-paramyxovirus-isolated-from-kidneys-of-bank-voles-in-russia
#6
Sergey Alkhovsky, Alexander Butenko, Aykaz Eremyan, Alexey Shchetinin
A genome of bank vole virus (BaVV), isolated from kidney tissues of bank voles (Myodes glareolus) in Russia in 1973, was sequenced. The genomic organization of BaVV (3'-N-P/V/C-M-F-G-L-5', 16,992 nt in length; GenBank accession number MF943130) is most similar to that of Mossman virus (MoV) and Nariva virus (NarPV), two ungrouped paramyxoviruses isolated from rodents in Australia and Trinidad, respectively. The proteins of BaVV have the highest level of sequence identity (ranging from 23-28% for G protein to 66-73% for M protein) to proteins of MoV and NarPV...
November 11, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28950869/phylogenetic-assessment-reveals-continuous-evolution-and-circulation-of-pigeon-derived-virulent-avian-avulaviruses-1-in-eastern-europe-asia-and-africa
#7
Mahmoud Sabra, Kiril M Dimitrov, Iryna V Goraichuk, Abdul Wajid, Poonam Sharma, Dawn Williams-Coplin, Asma Basharat, Shafqat F Rehmani, Denys V Muzyka, Patti J Miller, Claudio L Afonso
BACKGROUND: The remarkable diversity and mobility of Newcastle disease viruses (NDV) includes virulent viruses of genotype VI. These viruses are often referred to as pigeon paramyxoviruses 1 because they are normally isolated and cause clinical disease in birds from the Columbidae family. Genotype VI viruses occasionally infect, and may also cause clinical disease in poultry. Thus, the evolution, current spread and detection of these viruses are relevant to avian health. RESULTS: Here, we describe the isolation and genomic characterization of six Egyptian (2015), four Pakistani (2015), and two Ukrainian (2007, 2013) recent pigeon-derived NDV isolates of sub-genotype VIg...
September 26, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28840181/measles-virus-nucleocapsid-protein-modulates-the-signal-regulatory-protein-%C3%AE-1-sirp%C3%AE-1-to-enhance-osteoclast-differentiation-in-paget-s-disease-of-bone
#8
Kumaran Sundaram, Yuvaraj Sambandam, Srinivasan Shanmugarajan, D Sudhaker Rao, Sakamuri V Reddy
Paget's disease of bone (PDB) is a chronic localized bone disorder in an elderly population. Environmental factors such as paramyxovirus are implicated in PDB and measles virus nucleocapsid protein (MVNP) has been shown to induce pagetic osteoclasts (OCLs). However, the molecular mechanisms underlying MVNP stimulation of OCL differentiation in the PDB are unclear. We therefore determined the MVNP regulated gene expression profiling during OCL differentiation. Agilent microarray analysis of gene expression identified high levels of SIRPβ1 (353-fold) expression in MVNP transduced human bone marrow mononuclear cells stimulated with RANKL...
December 2017: Bone Reports
https://www.readbyqxmd.com/read/28835499/nipah-and-hendra-virus-nucleoproteins-inhibit-nuclear-accumulation-of-signal-transducer-and-activator-of-transcription-1-stat1-and-stat2-by-interfering-with-their-complex-formation
#9
Akihiro Sugai, Hiroki Sato, Ikuyo Takayama, Misako Yoneda, Chieko Kai
Henipaviruses, such as Nipah (NiV) and Hendra (HeV) viruses, are highly pathogenic zoonotic agents within the Paramyxoviridae family. The phosphoprotein (P) gene products of the paramyxoviruses have been well characterized for their interferon (IFN) antagonist activity and their contribution to viral pathogenicity. In this study, we demonstrated that the nucleoprotein (N) of henipaviruses also prevents the host IFN signaling response. Reporter assays demonstrated that the NiV and HeV N proteins (NiV-N and HeV-N, respectively) dose-dependently suppressed both type I and type II IFN responses and that the inhibitory effect was mediated by their core domains...
November 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28339499/evaluation-of-fusion-protein-cleavage-site-sequences-of-newcastle-disease-virus-in-genotype-matched-vaccines
#10
Shin-Hee Kim, Zongyan Chen, Asuka Yoshida, Anandan Paldurai, Sa Xiao, Siba K Samal
Newcastle disease virus (NDV) causes a devastating poultry disease worldwide. Frequent outbreaks of NDV in chickens vaccinated with conventional live vaccines suggest a need to develop new vaccines that are genetically matched against circulating NDV strains, such as the genotype V virulent strains currently circulating in Mexico and Central America. In this study, a reverse genetics system was developed for the virulent NDV strain Mexico/01/10 strain and used to generate highly attenuated vaccine candidates by individually modifying the cleavage site sequence of fusion (F) protein...
2017: PloS One
https://www.readbyqxmd.com/read/27622505/the-matrix-protein-of-nipah-virus-targets-the-e3-ubiquitin-ligase-trim6-to-inhibit-the-ikk%C3%AE%C2%B5-kinase-mediated-type-i-ifn-antiviral-response
#11
Preeti Bharaj, Yao E Wang, Brian E Dawes, Tatyana E Yun, Arnold Park, Benjamin Yen, Christopher F Basler, Alexander N Freiberg, Benhur Lee, Ricardo Rajsbaum
For efficient replication, viruses have developed mechanisms to evade innate immune responses, including the antiviral type-I interferon (IFN-I) system. Nipah virus (NiV), a highly pathogenic member of the Paramyxoviridae family (genus Henipavirus), is known to encode for four P gene-derived viral proteins (P/C/W/V) with IFN-I antagonist functions. Here we report that NiV matrix protein (NiV-M), which is important for virus assembly and budding, can also inhibit IFN-I responses. IFN-I production requires activation of multiple signaling components including the IκB kinase epsilon (IKKε)...
September 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27512068/human-ifit1-inhibits-mrna-translation-of-rubulaviruses-but-not-other-members-of-the-paramyxoviridae-family
#12
D F Young, J Andrejeva, X Li, F Inesta-Vaquera, C Dong, V H Cowling, S Goodbourn, R E Randall
UNLABELLED: We have previously shown that IFIT1 is primarily responsible for the antiviral action of interferon (IFN) alpha/beta against parainfluenza virus type 5 (PIV5), selectively inhibiting the translation of PIV5 mRNAs. Here we report that while PIV2, PIV5, and mumps virus (MuV) are sensitive to IFIT1, nonrubulavirus members of the paramyxoviridae such as PIV3, Sendai virus (SeV), and canine distemper virus (CDV) are resistant. The IFIT1 sensitivity of PIV5 was not rescued by coinfection with an IFIT1-resistant virus (PIV3), demonstrating that PIV3 does not specifically inhibit the antiviral activity of IFIT1 and that the inhibition of PIV5 mRNAs is regulated by cis-acting elements...
October 15, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27498841/roles-of-nuclear-trafficking-in-infection-by-cytoplasmic-negative-strand-rna-viruses-paramyxoviruses-and-beyond
#13
REVIEW
Michelle D Audsley, David A Jans, Gregory W Moseley
Genome replication and virion production by most negative-sense RNA viruses (NSVs) occurs exclusively in the cytoplasm, but many NSV-expressed proteins undergo active nucleocytoplasmic trafficking via signals that exploit cellular nuclear transport pathways. Nuclear trafficking has been reported both for NSV accessory proteins (including isoforms of the rabies virus phosphoprotein, and V, W and C proteins of paramyxoviruses) and for structural proteins. Trafficking of the former is thought to enable accessory functions in viral modulation of antiviral responses including the type I IFN system, but the intranuclear roles of structural proteins such as nucleocapsid and matrix proteins, which have critical roles in extranuclear replication and viral assembly, are less clear...
October 2016: Journal of General Virology
https://www.readbyqxmd.com/read/26982470/kinetic-analysis-of-rna-editing-of-newcastle-disease-virus-p-gene-in-the-early-period-of-infection
#14
X Qiu, Q Fu, C Meng, S Yu, Y Zhan, L Dong, T Ren, Y Sun, L Tan, C Song, X Han, C Ding
UNLABELLED: As a paramyxovirus, Newcastle disease virus (NDV) has the ability to edit its P (phosphoprotein) gene to synthesize three kinds of viral protein (P, V and W). It is technically very difficult to differentiate P, V and W mRNAs, and little was known about NDV regulation of RNA-editing frequency. To investigate the rules of NDV RNA editing, the ratio of the P gene-derived transcripts (P, V and W) was determined by sequencing at different time points post-infection. The results showed unstable ratio of V and W mRNA at different time points, and the frequency of NDV editing was significantly increased at the early period of infection (P  KEYWORDS: Newcastle disease virus; phosphoprotein; RNA editing; G insertion...
March 2016: Acta Virologica
https://www.readbyqxmd.com/read/26867212/multiple-novel-functions-of-henipavirus-o-glycans-the-first-o-glycan-functions-identified-in-the-paramyxovirus-family
#15
Jacquelyn A Stone, Anthony V Nicola, Linda G Baum, Hector C Aguilar
O-linked glycosylation is a ubiquitous protein modification in organisms belonging to several kingdoms. Both microbial and host protein glycans are used by many pathogens for host invasion and immune evasion, yet little is known about the roles of O-glycans in viral pathogenesis. Reportedly, there is no single function attributed to O-glycans for the significant paramyxovirus family. The paramyxovirus family includes many important pathogens, such as measles, mumps, parainfluenza, metapneumo- and the deadly Henipaviruses Nipah (NiV) and Hendra (HeV) viruses...
February 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/26859759/newcastle-disease-virus-v-protein-targets-phosphorylated-stat1-to-block-ifn-i-signaling
#16
Xusheng Qiu, Qiang Fu, Chunchun Meng, Shengqing Yu, Yuan Zhan, Luna Dong, Cuiping Song, Yingjie Sun, Lei Tan, Shunlin Hu, Xiaoquan Wang, Xiaowen Liu, Daxin Peng, Xiufan Liu, Chan Ding
Newcastle disease virus (NDV) V protein is considered as an effector for IFN antagonism, however, the mechanism remains unknown. In this study, the expression of STAT1 and phospho-STAT1 in cells infected with NDV or transfected with V protein-expressing plasmids were analyzed. Our results showed that NDV V protein targets phospho-STAT1 reduction in the cells depends on the stimulation of IFN-α. In addition, a V-deficient genotype VII recombinant NDV strain rZJ1-VS was constructed using reverse genetic technique to confirm the results...
2016: PloS One
https://www.readbyqxmd.com/read/26640816/oncolysis-by-paramyxoviruses-multiple-mechanisms-contribute-to-therapeutic-efficiency
#17
Olga V Matveeva, Zong S Guo, Svetlana A Shabalina, Peter M Chumakov
Oncolytic paramyxoviruses include some strains of Measles, Mumps, Newcastle disease, and Sendai viruses. All these viruses are well equipped for promoting highly specific and efficient malignant cell death, which can be direct and/or immuno-mediated. A number of proteins that serve as natural receptors for oncolytic paramyxoviruses are frequently overexpressed in malignant cells. Therefore, the preferential interaction of paramyxoviruses with malignant cells rather than with normal cells is promoted. Due to specific genetic defects of cancer cells in the interferon (IFN) and apoptotic pathways, viral replication has the potential to be promoted specifically in tumors...
2015: Molecular Therapy Oncolytics
https://www.readbyqxmd.com/read/26546155/la-piedad-michoac%C3%A3-n-mexico-virus-v-protein-antagonizes-type-i-interferon-response-by-binding-stat2-protein-and-preventing-stats-nuclear-translocation
#18
Giuseppe Pisanelli, Maudry Laurent-Rolle, Balaji Manicassamy, Alan Belicha-Villanueva, Juliet Morrison, Bernardo Lozano-Dubernard, Felipa Castro-Peralta, Giuseppe Iovane, Adolfo García-Sastre
La Piedad Michoacán Mexico Virus (LPMV) is a member of the Rubulavirus genus within the Paramyxoviridae family. LPMV is the etiologic agent of "blue eye disease", causing a significant disease burden in swine in Mexico with long-term implications for the agricultural industry. This virus mainly affects piglets and is characterized by meningoencephalitis and respiratory distress. It also affects adult pigs, causing reduced fertility and abortions in females, and orchitis and epididymitis in males. Viruses of the Paramyxoviridae family evade the innate immune response by targeting components of the interferon (IFN) signaling pathway...
February 2, 2016: Virus Research
https://www.readbyqxmd.com/read/26526590/the-non-pathogenic-henipavirus-cedar-paramyxovirus-phosphoprotein-has-a-compromised-ability-to-target-stat1-and-stat2
#19
Kim G Lieu, Glenn A Marsh, Lin-Fa Wang, Hans J Netter
Immune evasion by the lethal henipaviruses, Hendra (HeV) and Nipah virus, is mediated by its interferon (IFN) antagonist P gene products, phosphoprotein (P), and the related V and W proteins, which can target the signal transducer and activator of transcription 1 (STAT1) and STAT2 proteins to inhibit IFN/STAT signaling. However, it is not clear if the recently identified non-pathogenic Henipavirus, Cedar paramyxovirus (CedPV), is also able to antagonize the STAT proteins. We performed comparative studies between the HeV P gene products (P/V/W) and CedPV-P (CedPV does not encode V or W) and demonstrate that differences exist in their ability to engage the STAT proteins using immunoprecipitation and quantitative confocal microscopic analysis...
December 2015: Antiviral Research
https://www.readbyqxmd.com/read/26378167/regulation-of-viral-rna-synthesis-by-the-v-protein-of-parainfluenza-virus-5
#20
Yang Yang, James Zengel, Minghao Sun, Katrina Sleeman, Khalid Amine Timani, Jason Aligo, Paul Rota, Jianguo Wu, Biao He
UNLABELLED: Paramyxoviruses include many important animal and human pathogens. The genome of parainfluenza virus 5 (PIV5), a prototypical paramyxovirus, encodes a V protein that inhibits viral RNA synthesis. In this work, the mechanism of inhibition was investigated. Using mutational analysis and a minigenome system, we identified regions in the N and C termini of the V protein that inhibit viral RNA synthesis: one at the very N terminus of V and the second at the C terminus of V. Furthermore, we determined that residues L16 and I17 are critical for the inhibitory function of the N-terminal region of the V protein...
December 2015: Journal of Virology
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