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https://www.readbyqxmd.com/read/28619798/metagenomes-of-soil-samples-from-an-established-perennial-cropping-system-of-asparagus-treated-with-biostimulants-in-southern-france
#1
Julien Crovadore, Ali Asaff Torres, Raúl Rodríguez Heredia, Bastien Cochard, Romain Chablais, François Lefort
We report here the metagenomes of soil samples from a perennial cropping system of asparagus that was treated with two biostimulants. Two treatments were compared to an untreated control. Control soil samples were taken at the beginning and at end of the experiment.
June 15, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28573207/epidithiodiketopiperazines-strain-promoted-thiol-mediated-cellular-uptake-at-the-highest-tension
#2
Lili Zong, Eline Bartolami, Daniel Abegg, Alexander Adibekian, Naomi Sakai, Stefan Matile
The disulfide dihedral angle in epidithiodiketopiperazines (ETPs) is near 0°. Application of this highest possible ring tension to strain-promoted thiol-mediated uptake results in efficient delivery to the cytosol and nucleus. Compared to the previous best asparagusic acid (AspA), ring-opening disulfide exchange with ETPs occurs more efficiently even with nonactivated thiols, and the resulting thiols exchange rapidly with nonactivated disulfides. ETP-mediated cellular uptake is more than 20 times more efficient compared to AspA, occurs without endosomal capture, depends on temperature, and is "unstoppable" by inhibitors of endocytosis and conventional thiol-mediated uptake, including siRNA against the transferrin receptor...
May 24, 2017: ACS Central Science
https://www.readbyqxmd.com/read/28572584/comparative-de-novo-transcriptome-profiles-in-asparagus-officinalis-and-a-kiusianus-during-the-early-stage-of-phomopsis-asparagi-infection
#3
Mostafa Abdelrahman, Naoyuki Suzumura, Mai Mitoma, Satoshi Matsuo, Takao Ikeuchi, Mitsutaka Mori, Kyoko Murakami, Yukio Ozaki, Masaru Matsumoto, Atsuko Uragami, Akira Kanno
Asparagus kiusianus, an important wild relative of cultivated asparagus (A. officinalis), exhibits resistance to stem blight disease caused by Phomopsis asparagi. However, the mechanisms underlying this resistance are not understood and no transcriptomic or genetic resources are available for this species. De novo transcriptome sequencing of A. officinalis and A. kiusianus stems was performed 24 h after inoculation with P. asparagi. In total, 35,259 and 36,321 transcripts were annotated in A. officinalis and A...
June 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28530188/simple-assay-for-screening-phytoestrogenic-compounds-using-the-oestrogen-receptor-immobilised-magnetite-nanoparticles
#4
Pimchanok Busayapongchai, Sineenat Siri
With increasing interests of phytoestrogens for their potential applications, a rapid and simple tool for screening these phytochemicals is still required. In this study, a simple assay to detect phytoestrogens was developed based on the competition binding between the tested samples and the fluorescently labelled oestrogen (E2) to the human ligand binding domain of oestrogen receptor (LBD-ER) that was immobilised on the magnetite nanoparticles (MNPs). The 40-kDa LBD-ER peptide was produced in an Escherichia coli system...
June 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28439480/development-of-microsatellite-markers-based-on-expressed-sequence-tags-in-asparagus-cochinchinensis-asparagaceae
#5
Bo-Yun Kim, Han-Sol Park, Jung-Hoon Lee, Myounghai Kwak, Young-Dong Kim
PREMISE OF THE STUDY: Transcriptome-derived simple sequence repeat (SSR) markers were developed in Asparagus cochinchinensis (Asparagaceae). Due to its application in traditional medicine, its wild populations are threatened by over-collection even in protected areas, requiring immediate conservation efforts. METHODS AND RESULTS: Based on transcriptome data of A. cochinchinensis, 96 primer pairs with two to seven alleles per locus were selected for initial validation; of those, 27 primer pairs amplified across all samples, resulting in 15 polymorphic and 12 monomorphic microsatellite markers...
April 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28411810/multivariate-curve-resolution-applied-to-kinetic-spectroscopic-data-matrices-dye-determination-in-foods-by-means-of-enzymatic-oxidation
#6
Valeria Boeris, Juan A Arancibia, Alejandro C Olivieri
In this work, the combination of chemometric techniques with kinetic-spectroscopic data allowed quantifying two dyes (tartrazine and carminic acid) in complex matrices as mustard, ketchup, asparagus soup powder, pumpkin soup powder, plum jam and orange-strawberry juice. Quantitative analysis was performed without the use of tedious sample pretreatment, due to the achievement of the second-order advantage. The results obtained showed an improvement in simplicity, speed and cost with respect to usual separation techniques, allowing to properly quantifying these dyes obtaining limits of detection below 0...
July 1, 2017: Talanta
https://www.readbyqxmd.com/read/28388915/improved-curdlan-production-with-discarded-bottom-parts-of-asparagus-spear
#7
Rex Frimpong Anane, Huifang Sun, Lamei Zhao, Le Wang, Chun Lin, Zichao Mao
BACKGROUND: This work evaluated the improvement of curdlan production of Agrobacterium sp. ATCC 31749 by using culture medium containing juice of discarded bottom part of green Asparagus spear (MJDA). Curdlan production was carried out using Agrobacterium sp. ATCC 31749 in flasks with different volumes of MJDA and its non-juice-adding control (CK) incubated in shaker at 30 °C, 200 rpm rotation for 168 h. RESULTS: All MJDA media increased Agrobacterium sp. ATCC 31749 cell mass and enhanced the cells' ability to utilise sucrose, the carbon source for curdlan biosynthesis, and thereby produced higher concentration of curdlan than CK which is used for commercial production of curdlan...
April 7, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28380562/metals-and-metalloids-in-wild-asparagus-at-uncontaminated-and-mining-contaminated-sites
#8
R Biddau, R Cidu
Wild edible plants are often considered healthier than crops because they are unaffected by fertilizers, herbicides, and pesticides. However, the occurrence of potentially toxic elements in wild plants is poorly known and needs to be assessed. In this study, 22 elements were determined by inductively coupled plasma mass spectrometry in wild asparagus ( L.). Asparagus shoots were collected in uncontaminated environments (20 sites) and in areas contaminated by past mining (15 sites) in Sardinia, Italy. Beryllium, Bi, Te, Tl, and U were not detected in asparagus (<0...
March 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28341907/utilization-of-inulin-containing-waste-in-industrial-fermentations-to-produce-biofuels-and-bio-based-chemicals
#9
REVIEW
Stephen R Hughes, Nasib Qureshi, Juan Carlos López-Núñez, Marjorie A Jones, Joshua M Jarodsky, Luz Ángela Galindo-Leva, Mitchell R Lindquist
Inulins are polysaccharides that belong to an important class of carbohydrates known as fructans and are used by many plants as a means of storing energy. Inulins contain 20 to several thousand fructose units joined by β-2,1 glycosidic bonds, typically with a terminal glucose unit. Plants with high concentrations of inulin include: agave, asparagus, coffee, chicory, dahlia, dandelion, garlic, globe artichoke, Jerusalem artichoke, jicama, onion, wild yam, and yacón. To utilize inulin as its carbon and energy source directly, a microorganism requires an extracellular inulinase to hydrolyze the glycosidic bonds to release fermentable monosaccharides...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28314158/asparagus-decline-autotoxicity-and-autotoxic-compounds-in-asparagus-rhizomes
#10
Hisashi Kato-Noguchi, Keisuke Nakamura, Osamu Ohno, Kiyotake Suenaga, Nobuyuki Okuda
Asparagus (Asparagus officinalis L.) is a perennial vegetable, but its crop productivity and quality decrease gradually. One possible reason for "asparagus decline" is thought to be the autotoxicity of asparagus. However, the autotoxic property of asparagus rhizomes remains unknown. The objective of this study was to determine the potential role of rhizomes in the autotoxicity of asparagus. An aqueous methanol extract of asparagus rhizomes inhibited the growth of asparagus seedlings and six other test plants in a concentration-dependent manners: garden cress (Lepidum sativum L...
March 1, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28275781/aqueous-extracts-from-asparagus-stems-prevent-memory-impairments-in-scopolamine-treated-mice
#11
Zifang Sui, Ce Qi, Yunxiang Huang, Shufeng Ma, Xinguo Wang, Guowei Le, Jin Sun
Aqueous extracts from Asparagus officinalis L. stems (AEAS) are rich in polysaccharides, gamma-amino butyric acid (GABA), and steroidal saponin. This study was designed to investigate the effects of AEAS on learning, memory, and acetylcholinesterase-related activity in a scopolamine-induced model of amnesia. Sixty ICR mice were randomly divided into 6 groups (n = 10) including the control group (CT), scopolamine group (SC), donepezil group (DON), low, medium, and high dose groups of AEAS (LS, MS, HS; 1.6 mL kg(-1), 8 mL kg(-1), 16 mL kg(-1))...
April 19, 2017: Food & Function
https://www.readbyqxmd.com/read/28260011/ethyl-acetate-extract-from-asparagus%C3%A2-cochinchinensis-exerts-anti%C3%A2-inflammatory-effects-in-lps%C3%A2-stimulated-raw264-7-macrophage-cells-by-regulating-cox%C3%A2-2-inos-inflammatory-cytokine-expression-map-kinase-pathways-the-cell-cycle-and-anti-oxidant-activity
#12
Hyun Ah Lee, Eun Kyoung Koh, Ji Eun Sung, Ji Eun Kim, Sung Hwa Song, Dong Seob Kim, Hong Joo Son, Chung Yeoul Lee, Hee Seob Lee, Chang Joon Bae, Dae Youn Hwang
Asparagus cochinchinesis (A. cochinchinesis) is a medicine traditionally used to treat fever, cough, kidney disease, breast cancer, inflammatory disease and brain disease in northeast Asian countries. Although numerous studies of the anti‑inflammatory effects of A. cochinchinesis have been conducted, the underlying mechanisms of such effects in macrophages remain to be demonstrated. To investigate the mechanism of suppressive effects on the inflammatory response in macrophages, alterations of the nitric oxide (NO) level, the cell viability, inducible nitric oxide synthase (iNOS) and cyclooxygenase‑2 (COX‑2) expression levels, inflammatory cytokine expression, the mitogen-activated protein kinase (MAPK) signaling pathway, cell cycle arrest and reactive oxygen species (ROS) levels were measured in lipopolysaccharide (LPS)-activated RAW264...
April 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28244754/1-2-dithiolane-derived-dynamic-covalent-materials-cooperative-self-assembly-and-reversible-cross-linking
#13
Xiangyi Zhang, Robert M Waymouth
The use of dithiolane-containing polymers to construct responsive and dynamic networks is an attractive strategy in material design. Here, we provide a detailed mechanistic study on the self-assembly and gelation behavior of a class of ABA triblock copolymers containing a central poly(ethylene oxide) block and terminal polycarbonate blocks with pendant 1,2-dithiolane functionalities. In aqueous solution, these amphiphilic block copolymers self-assemble into bridged flower micelles at high concentrations. The addition of a thiol initiates the reversible ring-opening polymerizations of dithiolanes in the micellar cores to induce the cross-linking and gelation of the micellar network...
February 28, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28198114/immunoaffinity-knockout-of-saponin-glycosides-from-asparagus-racemosus-to-assess-anti-lipid-peroxidation
#14
Churanya Onlom, Watoo Phrompittayarat, Waraporn Putalun, Neti Waranuch, Kornkanok Ingkaninan
INTRODUCTION: Asparagus racemosus Willd (Asparagaceae family), known as Shatavari, is important in Ayurveda and traditional Thai medicines. The saponin glycosides, shatavarin I and IV are major constituents in its roots and may be responsible for their actions including protection against lipid peroxidation and carcinogenesis. OBJECTIVE: To develop an immunoaffinity column for isolating compounds with structures related to shatavarin IV from crude extracts of A...
February 15, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28182033/preliminary-screening-of-a-classical-ayurvedic-formulation-for-anticonvulsant-activity
#15
Arnab Dhar, Santosh Kumar Maurya, Ashish Mishra, Gireesh Kumar Singh, Manoj Kumar Singh, Ankit Seth
BACKGROUND: Epilepsy is a serious and complex central nervous system disorder associated with recurrent episodes of convulsive seizures due to the imbalance between excitatory (glutamatergic) and inhibitory (GABAergic) neurotransmitters level in the brain. The available treatments are neither competent to control the seizures nor prevent progress of disease. Since ages, Herbal medicines have remained important sources of medicines in many parts of world which is evidenced through their uses in traditional systems of medicine i...
July 2016: Ancient Science of Life
https://www.readbyqxmd.com/read/28176806/a-putative-myb35-ortholog-is-a-candidate-for-the-sex-determining-genes-in-asparagus-officinalis
#16
Daisuke Tsugama, Kohei Matsuyama, Mayui Ide, Masato Hayashi, Kaien Fujino, Kiyoshi Masuda
Asparagus officinalis (garden asparagus) is a dioecious perennial crop. For agricultural production of A. officinalis, male plants have advantages over female plants. The dioecism of A. officinalis is determined by the single dominant masculinizing M locus, which is involved in tapetal cell development in stamens, but thus far no specific M locus genes have been identified. We re-analyzed previously published RNA-Seq data for the A. officinalis transcriptome, cloned some genes, and discovered that a putative ortholog of MYB35, which is indispensable for tapetal cell development in Arabidopsis thaliana, is absent in the genome of female plants in A...
February 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28152205/saponin-profile-of-wild-asparagus-species
#17
Sara Jaramillo-Carmona, Rocío Rodriguez-Arcos, Ana Jiménez-Araujo, Sergio López, Juan Gil, Roberto Moreno, Rafael Guillén-Bejarano
The aim of this work was to study the saponin profiles from spears of different wild asparagus species in the context of its genetic diversity aside from geographical seed origin. They included Asparagus pseudoscaber Grecescu, Asparagus maritimus (L.) Mill., Asparagus brachiphyllus Turcz., Asparagus prostrates Dumort., and Asparagus officinalis L. The saponin analysis by LC-MS has shown that saponin profile from wild asparagus is similar to that previously described for triguero asparagus from Huétor-Tájar landrace (triguero HT), which had not ever been reported in the edible part of asparagus...
March 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28071026/antiradical-capacity-and-polyphenol-composition-of-asparagus-spears-varieties-cultivated-under-different-sunlight-conditions
#18
Bartosz Kulczyński, Joanna Kobus-Cisowska, Dominik Kmiecik, Anna Gramza-Michałowska, Dorota Golczak, Józef Korczak
BACKGROUND: Asparagus officinalis has a high nutritional value. Asparagus is rich in a number of bioactive compounds, mainly flavonoids (quercetin), glutathione, vitamin C, vitamin E, fructans (inulin and fructooligosaccharides) and phytosterols (b-sitosterol). These compounds may play an important role in human health. The purpose of this study was to examine the antioxidant potential and polyphenol composition of white, pale-colored and green asparagus spears of different cultivars...
July 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28024292/adjuvant-effect-of-asparagus-racemosus-willd-derived-saponins-in-antibody-production-allergic-response-and-pro-inflammatory-cytokine-modulation
#19
Nimisha Tiwari, Vivek Kumar Gupta, Pallavi Pandey, Dinesh Kumar Patel, Suchitra Banerjee, Mahendra Pandurang Darokar, Anirban Pal
The study manifests the immunoadjuvant potential of saponin rich fraction from Asparagus racemosus in terms of cellular and humoral immune response that can be exploited against microbial infections. Asparagus racemosus (AR) has been attributed as an adaptogen and rasayana in traditional medication systems for enhancing the host defence mechanism. Spectrophotometric and HPTLC analysis ensured the presence of saponins. The saponin rich fractions were tested for immunoadjuvant property in ovalbumin immunised mice for the humoral response, quantified in terms of prolonged antibody production upto a duration of 56days...
February 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28001050/strained-cyclic-disulfides-enable-cellular-uptake-by-reacting-with-the-transferrin-receptor
#20
Daniel Abegg, Giulio Gasparini, Dominic G Hoch, Anton Shuster, Eline Bartolami, Stefan Matile, Alexander Adibekian
In this study, we demonstrate that appendage of a single asparagusic acid residue (AspA tag) is sufficient to ensure efficient cellular uptake and intracellular distribution of fully unprotected peptides. We apply this new delivery method to induce apoptotic response in cancer cells using long (up to 20mer) BH3 domain peptides. Moreover, to understand the molecular mechanism of the cellular uptake, we perform chemical proteomics experiments and identify the direct molecular targets of the asparagusic acid tag...
January 11, 2017: Journal of the American Chemical Society
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