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https://www.readbyqxmd.com/read/27921101/the-effect-of-common-anticoagulants-in-detection-and-quantification-of-malaria-parasitemia-in-human-red-blood-cells-by-atr-ftir-spectroscopy
#1
Miguela Martin, David Perez-Guaita, Dean W Andrew, Jack S Richards, Bayden R Wood, Philip Heraud
Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) has the potential to become a new diagnostic tool for malaria and other diseases. For point-of-care testing, the use of ATR-FTIR in malaria diagnosis enables the analysis of blood in the aqueous state, which represents an enormous advantage by minimising the sample preparation by removing the need for cell fixation. Here we report the quantification of malaria parasitemia in human RBCs in their normal physiological aqueous state...
December 6, 2016: Analyst
https://www.readbyqxmd.com/read/27917178/a-comparative-nitrogen-balance-and-productivity-analysis-of-legume-and-non-legume-supported-cropping-systems-the-potential-role-of-biological-nitrogen-fixation
#2
Pietro P M Iannetta, Mark Young, Johann Bachinger, Göran Bergkvist, Jordi Doltra, Rafael J Lopez-Bellido, Michele Monti, Valentini A Pappa, Moritz Reckling, Cairistiona F E Topp, Robin L Walker, Robert M Rees, Christine A Watson, Euan K James, Geoffrey R Squire, Graham S Begg
The potential of biological nitrogen fixation (BNF) to provide sufficient N for production has encouraged re-appraisal of cropping systems that deploy legumes. It has been argued that legume-derived N can maintain productivity as an alternative to the application of mineral fertilizer, although few studies have systematically evaluated the effect of optimizing the balance between legumes and non N-fixing crops to optimize production. In addition, the shortage, or even absence in some regions, of measurements of BNF in crops and forages severely limits the ability to design and evaluate new legume-based agroecosystems...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27914666/reprint-of-spatial-modeling-of-cutaneous-leishmaniasis-in-iran-from-1983-to-2013
#3
Kourosh Holakouie-Naieni, Ehsan Mostafavi, Ali Darvishi Boloorani, Mehdi Mohebali, Reza Pakzad
INTRODUCTION: Cutaneous Leshmaniasis (CL), a parasitic skin infection caused by Leishmania species, is endemic in some regions of Iran. In this study, the effect of location on the incidence and distribution of CL in Iran was studied. METHODS: We collected datas including the number of Cutaneous Leishmaniasis cases and populations at-risk of disease in Iran's different provinces reported by the Iranian ministry of health and the National Bureau of Statistics, respectively...
November 30, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27912754/bovine-mastitis-prevalence-risk-factors-and-isolation-of-staphylococcus-aureus-in-dairy-herds-at-hawassa-milk-shed-south-ethiopia
#4
Rahmeto Abebe, Hagere Hatiya, Mesele Abera, Bekele Megersa, Kassahun Asmare
BACKGROUND: Mastitis is a disease of major economic importance in dairy industry worldwide. It is of particular concern in developing countries like Ethiopia, where milk and milk products are scarce. The objectives of the study were to estimate the prevalence of mastitis, identify the cow-and herd-level potential risk factors and isolate Staphylococcus aureus, one of etiological agents for contagious mastitis, from cows positive for mastitis. A total of 529 lactating cows selected randomly from 95 herds were screened by California mastitis test (CMT) for sub-clinical mastitis...
December 3, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27908699/fast-methodology-for-the-determination-of-a-broad-set-of-antibiotics-and-some-of-their-metabolites-in-seafood
#5
Albert Serra-Compte, Diana Álvarez-Muñoz, Sara Rodríguez-Mozaz, Damià Barceló
The presence of antibiotics in seafood for human consumption may pose a risk for consumers. A methodology for the analysis of antibiotics in seafood based on QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction, followed by detection and quantification using liquid chromatography coupled to mass spectrometry was developed. The analytical method was evaluated for the determination of 23 antibiotics (including parent compounds and some metabolites) in fish, mussels and clams. Recoveries ranged between 30% and 70% for most of the compounds and method detection and quantification limits (MDLs and MQLs) were between 0...
November 28, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27903591/platelet-rich-plasma-powder-a-new-preparation-method-for-the-standardization-of-growth-factor-concentrations
#6
Matthias Kieb, Frank Sander, Cornelia Prinz, Stefanie Adam, Anett Mau-Möller, Rainer Bader, Kirsten Peters, Thomas Tischer
BACKGROUND: Platelet-rich plasma (PRP) is widely used in sports medicine. Available PRP preparations differ in white blood cell, platelet, and growth factor concentrations, making standardized research and clinical application challenging. PURPOSE: To characterize a newly standardized procedure for pooled PRP that provides defined growth factor concentrations. STUDY DESIGN: Controlled laboratory study. METHODS: A standardized growth factor preparation (lyophilized PRP powder) was prepared using 12 pooled platelet concentrates (PCs) derived from different donors via apheresis...
November 30, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27901198/diversity-of-anurans-in-dry-forest-fragments-of-a-subtropical-region-in-brazil
#7
Eduardo J Dos Santos, Carlos E Conte
This study aimed to identify and compare the diversity between two areas within different matrices located in the Mesophytic Semideciduous Forest; to compare composition and diversity registered to other assemblages; and to evaluate which criteria used here were the most important when sampling them. Data was obtained from September 2011 to March 2012 from 10 sites with different habitats in two areas of mid northern state of Paraná, southern Brazil. We recorded 18 species. Alfa-diversity between the areas did not vary, but the population within one of them was more stable, suggesting better distribution and equity of species in the available habitat...
2016: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/27900225/internal-jugular-vein-thrombosis-in-isolated-tuberculous-cervical-lymphadenopathy
#8
Sanjay Khaladkar, Avadhesh Chauhan, Arijit Ghosh, Kunaal Jain, Surbhi Chauhan
Tuberculosis is a common infectious disease with a high prevalence in developing countries and presents a major public health issue. Internal jugular vein (IJV) thrombosis is a rare complication in tuberculous cervical lymphadenopathy. We report a case of 26-year male patient with a history of low-grade evening rise in fever, dry cough, loss of appetite, and loss of weight with swelling in lower neck on right side. Ultrasonography (USG) neck showed well-defined hypoechoic lymph nodes posterior to right IJV and common carotid artery in the lower neck at level IV and in the right supraclavicular region showing central necrotic areas with adjoining IJV thrombosis...
2016: Case Reports in Radiology
https://www.readbyqxmd.com/read/27899175/bioaccumulation-of-sediment-bound-dichlorodiphenyltrichloroethane-and-heavy-metals-in-benthic-polychaete-nereis-succinea-from-a-typical-mariculture-zone-in-south-china
#9
Huizhen Li, Fei Wang, Jing You
: Bioaccumulation potential and associated ecological risk of sediment-bound DDT and its metabolites (DDXs) and heavy metals in Hailing Bay, a typical mariculture zone along the southern coast of China, were evaluated. The estuarine sediments were co-polluted by DDXs (120-4882ng/g dry wt.) and heavy metals (292-409mg/kg dry wt.). Both DDXs and metals in sediment were bioavailable to Nereis succinea, although the biota-sediment accumulation factors were less than 1 except for DDE. Significant transformation of DDT in organism was observed and DDE was the main metabolite...
November 26, 2016: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/27895707/fungal-fermentation-on-anaerobic-digestate-for-lipid-based-biofuel-production
#10
Yuan Zhong, Zhiguo Liu, Christine Isaguirre, Yan Liu, Wei Liao
BACKGROUND: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27894697/an-innovative-ultrasound-assisted-extraction-micro-scale-cell-combined-with-gas-chromatography-mass-spectrometry-in-negative-chemical-ionization-to-determine-persistent-organic-pollutants-in-air-particulate-matter
#11
E Beristain-Montiel, R Villalobos-Pietrini, G E Arias-Loaiza, S L Gómez-Arroyo, O Amador-Muñoz
New clean technologies are needed to determine concentration of organic pollutants without generating more pollution. A method to extract Persistent Organic Pollutants (POPs) from airborne particulate matter was developed using a novel technology recently patented called ultrasound assisted extraction micro-scale cell (UAE-MSC). This technology extracts, filters, collects the sample, and evaporates the solvent, on-line. No sample transfer is needed. The cell minimizes sample manipulation, solvent consumption, waste generation, time, and energy; fulfilling most of the analytical green chemistry protocol...
November 23, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27893066/symptoms-and-satisfaction-of-patients-in-the-patient-reported-outcomes-with-laser-in-situ-keratomileusis-prowl-studies
#12
Malvina Eydelman, Gene Hilmantel, Michelle E Tarver, Elizabeth M Hofmeister, Jeanine May, Keri Hammel, Ron D Hays, Frederick Ferris
Importance: Patient-reported outcomes should be collected using validated questionnaires prior to and following laser in situ keratomileusis (LASIK) surgery. Objective: To report the frequency of patient-reported visual symptoms, dry eye symptoms, satisfaction with vision, and satisfaction with LASIK surgery in the Patient-Reported Outcomes With LASIK (PROWL) studies. Design, Setting, and Participants: The PROWL-1 and PROWL-2 studies were prospective, observational studies conducted from September 13, 2011, to June 27, 2014...
November 23, 2016: JAMA Ophthalmology
https://www.readbyqxmd.com/read/27893063/assessment-of-the-psychometric-properties-of-a-questionnaire-assessing-patient-reported-outcomes-with-laser-in-situ-keratomileusis-prowl
#13
Ron D Hays, Michelle E Tarver, Karen L Spritzer, Steve Reise, Gene Hilmantel, Elizabeth M Hofmeister, Keri Hammel, Jeanine May, Frederick Ferris, Malvina Eydelman
Importance: Patient-reported outcome (PRO) measures for laser in situ keratomileusis (LASIK) are needed. Objective: To develop PRO measures to assess satisfaction, eye-related symptoms, and their effect on functioning and well-being following LASIK based on patient and expert input. Design, Setting, and Participants: The Patient-Reported Outcomes With LASIK (PROWL) studies were prospective observational studies of patients undergoing LASIK surgery for myopia, hyperopia, or astigmatism...
November 23, 2016: JAMA Ophthalmology
https://www.readbyqxmd.com/read/27891036/complete-morphometric-analysis-of-jugular-foramen-and-its-clinical-implications
#14
Sushant Swaroop Das, Sandeep Saluja, Neelam Vasudeva
INTRODUCTION: Tumors affecting structures in the vicinity of jugular foramen such as glomus jugulare require microsurgical approach to access this region. These tumors tend to alter the normal architecture of the jugular foramen by invading it. Therefore, it is not feasible to have correct anatomic visualization of the foramen in the presence of such pathologies. Hence, a comprehensive knowledge of the jugular foramen is needed by all the neurosurgeons while doing surgery in this region...
October 2016: Journal of Craniovertebral Junction and Spine
https://www.readbyqxmd.com/read/27890244/development-and-optimization-of-a-wet-granulation-process-at-elevated-temperature-for-a-poorly-compactible-drug-using-twin-screw-extruder-for-continuous-manufacturing
#15
Anuprabha K Meena, Divyakant Desai, Abu T M Serajuddin
The objective of this study was to enhance tabletability of a poorly compactible drug, acetaminophen, by wet granulation using twin screw extruder at high temperature. It was desired that there would be minimum amounts of excipients used and the granules obtained after extrusion would be dry and fall within a size range suitable for tableting without any further processing. Mixtures of acetaminophen (95%) with binders (5% povidone or partially pregelatinized starch) were wet granulated through twin screw extruder at 70°C by adding 7% w/w water...
November 24, 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27889933/development-and-field-performance-of-nitrogen-use-efficient-rice-lines-for-africa
#16
Michael Gomez Selvaraj, Milton Orlando Valencia, Satoshi Ogawa, Yingzhi Lu, Liying Wu, Christopher Downs, Wayne Skinner, Zhongjin Lu, Jean C Kridl, Manabu Ishitani, Jos van Boxtel
Nitrogen (N) fertilizers are a major input cost in rice production and its excess application leads to major environmental pollution. Development of rice varieties with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. Here, we report the results of field evaluations of marker-free transgenic NERICA4 (New Rice for Africa 4) rice lines over-expressing barley alanine amino transferase (HvAlaAT) under the control of a rice stress-inducible promoter (pOsAnt1). Field evaluations over three growing seasons and two rice growing ecologies (lowland and upland) revealed that grain yield of pOsAnt1:HvAlaAT transgenic events was significantly higher than sibling nulls and wild type controls under different N application rates...
November 27, 2016: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/27889512/genotoxicity-and-growth-inhibition-effects-of-aniline-on-wheat
#17
Nan Tao, Guanyi Liu, Lu Bai, Lu Tang, Changhong Guo
Aniline is a synthetic compound widely used in industrial and pesticide production, which can lead to environmental pollution. Its high concentration in rivers and lakes is hazardous to aquatic species. Although the mechanism of aniline toxicity has been studied extensively in animals and algae, little is known about its genotoxicity in plants. In this study, we investigated the genotoxicity effects of aniline on wheat root tip cells. The mitotic index of wheat root tip cells decreased when the aniline test concentration was higher than 10 mg L(-1)...
November 24, 2016: Chemosphere
https://www.readbyqxmd.com/read/27889125/analysis-of-pasture-supplementation-strategies-by-means-of-a-mechanistic-model-of-ruminal-digestion-and-metabolism-in-the-dairy-cow
#18
J P McNamara, M J Auldist, L C Marett, P J Moate, W J Wales
Effective pasture supplementation is critical to the efficiency of resource management in milk production. We understand a great deal about ruminal and metabolic processes in dairy cattle that control efficiency but we need to improve our ability to predict effects of practical feeding strategies based on the basic biological processes of the cow. Therefore, a large-scale pasture supplementation study was used to explore the details of both practical management and the underlying biological principles and processes involved...
November 23, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27887656/looking-beyond-the-cancer-cell-for-effective-drug-combinations
#19
Jonathan R Dry, Mi Yang, Julio Saez-Rodriguez
Combinations of therapies are being actively pursued to expand therapeutic options and deal with cancer's pervasive resistance to treatment. Research efforts to discover effective combination treatments have focused on drugs targeting intracellular processes of the cancer cells and in particular on small molecules that target aberrant kinases. Accordingly, most of the computational methods used to study, predict, and develop drug combinations concentrate on these modes of action and signaling processes within the cancer cell...
November 25, 2016: Genome Medicine
https://www.readbyqxmd.com/read/27883144/robust-synthesis-of-f-bodipys
#20
Michael Helmut Reynolds Beh, Katherine Isabelle Biehn Douglas, Kelsey Taylor Elver House, Alexandra Celeste Murphy, Jackson Scott Testemale Sinclair, Alison Thompson
A protocol is established for the high-yielding synthesis of F-BODIPYs involving non-anhydrous reagents and not requiring precautions to exclude moisture. This simple and robust strategy simply requires a second addition of NEt3 and BF3·OEt2, midway through the reaction period. The ratio and amounts of NEt3 and BF3·OEt2 used in each aliquot are critical to success (6 : 9 for each aliquot). The protocol can be completed using bench-dry apparatus, without need to achieve and maintain anhydrous conditions or solvents...
November 24, 2016: Organic & Biomolecular Chemistry
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