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Wen Xu, Quankun Zhuang, Xia Chen, Ji Jiang, Pei Hu, Hongyun Wang
A robust ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of morphine-6-D-glucuronide (M6G), morphine-3-D-glucuronide (M3G) and morphine (MOR) in human plasma and urine has been developed and validated. The analytes of interest were extracted from plasma by protein precipitation. Urine sample was prepared by dilution. Both plasma and urine samples were chromatographed on an Acquity UPLC HSS T3 column using gradient elution. Detection was performed on a Xevo TQ-S tandem mass spectrometer in multiple reactions monitoring (MRM) mode using positive electrospray ionization (ESI)...
August 19, 2017: Biomedical Chromatography: BMC
Stanislav Kislyuk, Jerome Kroonen, Erwin Adams, Patrick Augustijns, Peter de Witte, Deirdre Cabooter
An analytical procedure to measure the whole-body uptake of pharmaceuticals in zebrafish has been developed using state-of-the-art methodologies. A sample preparation procedure for 9 pharmaceuticals displaying a variety in physicochemical properties was developed using 10-day old zebrafish (TG898). For an efficient homogenization of the samples and subsequent recovery of the compounds of interest, different amounts of organic solvents in combination with acidic modifiers were added to zebrafish samples. Samples were subsequently processed using a powerful bath sonicator and centrifuged...
November 1, 2017: Talanta
Johanna M Lindner, Michael Vogeser, Stefanie H Grimm
The measurement of steroid hormones and their corticoid precursors is an important aspect in endocrinology since these analytes are biomarkers for several endocrine disorders. Over the last few years, HPLC-MS/MS has become the method of choice to analyze these compounds. There are already several methods using stationary phases modified with C18 groups. However, since these columns sometimes do not enable sufficient separation of some isobaric steroids, we investigated the potential of a different RP modification using biphenyl groups for the separation of challenging isobars such as corticosterone, 11- and 21-deoxycortisol...
April 19, 2017: Journal of Pharmaceutical and Biomedical Analysis
Pengqiang Du, Xiaohu Wu, Jun Xu, Fengshou Dong, Xingang Liu, Dongmei Wei, Yongquan Zheng
In this study, an efficient and fast method for the determination of the herbicide mesotrione and its two metabolites [4-methylsulfonyl-2-nitrobenzoic acid (MNBA) and 2-amino-4-methylsulfonylbenzoic acid (AMBA)] in rice, rice plants, rice hulls, water, and soil was developed, using ultra performance liquid chromatography coupled with tandem quadrupole (Xevo TQ-S) mass spectrometry (UPLC-Xevo TQ-S-MS/MS). The recoveries of the compounds were 68.6%-108.4% at different levels. The intra-day relative standard deviation (RSD) ranged from 2...
August 15, 2017: Food Chemistry
Vaida Šulniūtė, Audrius Pukalskas, Petras Rimantas Venskutonis
Ten Salvia species, S. amplexicaulis, S. austriaca, S. forsskaolii S. glutinosa, S. nemorosa, S. officinalis, S. pratensis, S. sclarea, S. stepposa and S. verticillata were fractionated using supercritical carbon dioxide and pressurized liquid (ethanol and water) extractions. Fifteen phytochemicals were identified using commercial standards (some other compounds were identified tentatively), 11 of them were quantified by ultra high pressure chromatography (UPLC) with quadruple and time-of-flight mass spectrometry (Q/TOF, TQ-S)...
June 1, 2017: Food Chemistry
Helen M Talbot, Frances R Sidgwick, Juliane Bischoff, Kate A Osborne, Darci Rush, Angela Sherry, Charlotte L Spencer-Jones
RATIONALE: Traditional investigation of bacteriohopanepolyols (BHPs) has relied on derivatisation by acetylation prior to gas chromatography/mass spectrometry (GC/MS) or liquid chromatography/MS (LC/MS) analysis. Here, modern chromatographic techniques (ultrahigh-performance liquid chromatography (UPLC)) and new column chemistries were tested to develop a method for BHP analysis without the need for derivatisation. METHODS: Bacterial culture and sedimentary lipid extracts were analysed using a Waters Acquity Xevo TQ-S triple quadrupole mass spectrometer in positive ion atmospheric pressure chemical ionisation (APCI) mode...
October 15, 2016: Rapid Communications in Mass Spectrometry: RCM
Surekha Ramachandran, Sumathi Thangarajan
Huntington's disease (HD), a devastating neurodegenerative disease causing a remarkable pathogenesis involves mitochondrial dysfunction and bioenergetics failure. 3-Nitropropionic acid (3-NP) is a unique toxin model of HD that are mainly confined to mitochondrial complex-II inhibition and free radical generation. Recently, several nanoparticle formulations were developed to treat against various neurodegenerative diseases including HD. One among them is solid lipid nanoparticles (SLNs), a colloidal carrier designed to enhance the brain drug delivery and to prolong the bio-availability of drugs in the system...
August 25, 2016: Chemico-biological Interactions
Haiyan Liu, Hongjiang Xu, Wei Song, Yinsheng Zhang, Sen Yu, Xin Huang
Rapid, sensitive, selective and accurate ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of elbasvir (ELB) in rat plasma with deuterated elbasvir (ELB-D6) as internal standard (IS).Sample preparation was done by protein precipitation using acetonitrile containing 50 ng/mL IS. Chromatographic separation was achieved by an UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) column with a gradient mobile phase consisting of acetonitrile-water (containing 5...
March 15, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Takahiro Takayama, Haruhito Tsutsui, Ippei Shimizu, Tatsuya Toyama, Nobuyasu Yoshimoto, Yumi Endo, Koichi Inoue, Kenichiro Todoroki, Jun Zhe Min, Hajime Mizuno, Toshimasa Toyo'oka
BACKGROUND: Breast cancer is one of the most fearful diseases due to its increasing worldwide prevalence. A number of screening tests has been employed including clinical examinations and mammography. However, another screening method, which is a simple, not embarrassing, and low cost, is highly desired. Based on these findings, we are currently investigating the determination of polyamines including their acetylated structures for the diagnosis of breast cancer patients. We established a diagnostic approach to breast cancer patients based on the ratios of polyamines in saliva by a UPLC-MS/MS analysis...
January 15, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
Hera Chaudhry, Nida Fatima, Iffat Zareen Ahmad
Nigella sativa L. (family Ranunculaceae) is an annual herb of immense medicinal properties because of its major active components (i.e., thymoquinone (TQ), thymohydroquinone (THQ), and thymol (THY)). Plant tissue culture techniques like elicitation, Agrobacterium mediated transformation, hairy root culture, and so on, are applied for substantial metabolite production. This study enumerates the antibacterial and antioxidant potentials of N. sativa epicotyl suspension cultures under biotic and abiotic elicitation along with concentration optimization of the elicitors for enhanced TQ and THY production...
2015: BioMed Research International
Rana Keyhanmanesh, Saeideh Saadat, Mostafa Mohammadi, Amir-Ali Shahbazfar, Maryam Fallahi
In the present study, the preventive effect of two different concentrations of α-hederin, the active constituent of Nigella sativa, on lung inflammation and blood cytokines in ovalbumin sensitized guinea pigs was examined. Forty eight male adult guinea pigs were divided into control (C), sensitized (S) and sensitized pretreated groups; with thymoquinone (S+TQ), low dose (S+LAH) and high dose of α-hederin (S+HAH) and inhaled fluticasone propionate (S+FP). The lung histopathology and blood levels of IL-4, IFN-γ and IL-17 were assessed...
November 2015: Phytotherapy Research: PTR
Ke Li, Tyler J Buchinger, Ugo Bussy, Skye D Fissette, Nicholas S Johnson, Weiming Li
Many fishes are hypothesized to use bile acids (BAs) as chemical cues, yet quantification of BAs in biological samples and the required methods remain limited. Here, we present an UHPLC-MS/MS method for simultaneous, sensitive, and rapid quantification of 15 BAs, including free, taurine, and glycine conjugated BAs, and application of the method to fecal samples from lake charr (Salvelinus namaycush). The analytes were separated on a C18 column with acetonitrile-water (containing 7.5mM ammonium acetate and 0...
September 15, 2015: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Magdalena Wszedybyl-Winklewska, Jacek Wolf, Ewa Swierblewska, Katarzyna Kunicka, Marcin Gruszecki, Wojciech Guminski, Pawel J Winklewski, Andrzej F Frydrychowski, Leszek Bieniaszewski, Krzysztof Narkiewicz
BACKGROUND: Little is known about intracranial pressure (ICP)-cerebral haemodynamic interplay during repetitive apnoea. A recently developed method based on near-infrared transillumination/backscattering sounding (NIR-T/BSS) noninvasively measures changes in pial artery pulsation (cc-TQ) as well as subarachnoid width (sas-TQ) in humans. METHOD: We tested the complex response of the pial artery and subarachnoid width to apnoea using this method. The pial artery and subarachnoid width response to consecutive apnoeas lasting 30, 60 s and maximal breath-hold (91...
September 2015: Journal of Hypertension
Saeed Samarghandian, Mohsen Azimi-Nezhad, Hassan Mehrad-Majd, Seyed Reza Mirhafez
BACKGROUND: Gentamicin (GEN) is considered as a main aminoglycoside antibiotic medicine. The top therapeutic side effect of GEN is nephrotoxicity. The current research was proposed to determine the protective effect of thymoquinone (TQ) on GEN-induced acute renal failure (ARF). METHODS: The rats were divided into 6 groups: sham group, control group, GEN-treated group and the TQ-treated groups. At the end of the research period, the serum blood urea nitrogen (BUN) and creatinine (Cr) were measured...
2015: Pharmacology
Ülkü Mete Ural, Yeşim Bayoğlu Tekin, İbrahim Şehitoğlu, Yıldıray Kalkan, Medine Cumhur Cüre
AIM: To evaluate the antioxidant effects of thymoquinone (TQ) and to investigate the biochemical, histopathological and immunohistochemical changes in experimental rat ovarian torsion. METHODS: A total of 48 female adult rats were used in this study and randomly divided into 7 groups: (1) sham operation; (2) bilateral 3-hour ovarian ischemia; (3) 3-hour ischemia and 3-hour reperfusion; (4) and (5) rats were administered 20 and 40 mg/kg of TQ, respectively, before 0...
2016: Gynecologic and Obstetric Investigation
Ting Zhou, Shan Liu, Ting Zhao, Jing Zeng, Mingzhi He, Beining Xu, Shanshan Qu, Ling Xu, Wen Tan
A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for simultaneous chiral analysis of an antiasthma drug bambuterol, its key intermediate monocarbamate bambuterol and its active drug terbutaline in human plasma. All samples were extracted with ethyl acetate and separated on an Astec Chirobiotic T column under isocratic elution with a mobile phase consisting of methanol and water with the addition of 20mm ammonium acetate and 0.005% (v/v) formic acid at 0.6mL/min...
August 1, 2015: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
M Ayan, U Tas, E Sogut, S Caylı, H Kaya, M Esen, F Erdemir, M Uysal
We aimed to determine the protective effects of thymoquinone (TQ), against ischaemia-reperfusion (I/R) injury in the testis tissue of rats. Twenty-seven male Wistar albino rats were randomly divided into three equal groups as follows: Group I, sham group; Group II, torsion group; and Group III, torsion + thymoquinone group. The ischaemia period was 2 h, and orchiectomy was performed after 30 min of detorsion. Testis tissue sections were analysed with the terminal transferase mediated dUTP-nick end labelling (TUNEL) assay to determine in situ apoptotic DNA fragmentation...
March 2016: Andrologia
Omar Patiño-Rodríguez, Juan Ramón Zapata-Morales, Abraham Escobedo-Moratilla, Manuel Díaz de León-Cabrero, Irma Torres-Roque, José Pérez-Urizar
A high-throughput ultra-performance liquid chromatography coupled to tandem mass spectrometry (LC-ESI-MS-MS) method was developed for the determination of pinaverium bromide in human plasma. Protein precipitation with acetonitrile was used to extract pinaverium and itraconazole (as internal standard) from 500 µL plasma samples. The chromatographic separation was achieved with an Acquity UPLC BEH C18 column (1.7 µm, 2.1 × 100 mm) using a mixture of acetonitrile-5 mM ammonium formate (80:20, v/v) as mobile phase...
September 2015: Journal of Chromatographic Science
Sabah A A Linjawi, Wagdy K B Khalil, Mahrosa M Hassanane, Ekram S Ahmed
INTRODUCTION: The historical use of black cumin seed (Nigella sativa) dates back centuries, being embedded in Arabian culture and having a long history of unsurpassed medicinal value with versatility to treat a wide range of ailments. Thymoquinone (TQ) is now known to be the primary active constituent of black cumin seed oil (BCS oil) responsible for its medicinal effects and also showing promise for treatment of cancer. MATERIAL AND METHODS: In the current study, we have studied the effects of TQ and BCS oil on tumor markers (MDA, LDH, ALP and AST), histopathological alterations and the regulation of several genes (Brca1, Brca2, Id-1 and P53 mutation) related to breast cancer in female rats induced by 7,12-dimethylbenz[a]anthracene (DMBA) treatment...
March 16, 2015: Archives of Medical Science: AMS
Laleh Pejman, Hasan Omrani, Zahra Mirzamohammadi, Rana Keyhanmanesh
OBJECTIVES: For determining the mechanism of anti-asthmatic effect of thymoquinone, this investigation evaluated the effect of thymoquinone in the presence of selective A2A and A2B adenosine receptor antagonists (ZM241385 and MRS1706, respectively). MATERIALS AND METHODS: Seventy guinea pigs were randomly divided to 7 groups; control (C), sensitized with ovalbumin (S), sensitized groups pretreated with thymoquinone (S+TQ), ZM241385 (S+Anta A2A), MRS1706 (S+Anta A2B), thymoquinone and antagonists (S+Anta A2A+TQ and S+Anta A2B+TQ)...
December 2014: Iranian Journal of Basic Medical Sciences
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