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https://www.readbyqxmd.com/read/29223048/extraction-protocol-and-liquid-chromatography-tandem-mass-spectrometry-method-for-determining-micelle-entrapped-paclitaxel-at-the-cellular-and-subcellular-levels-application-to-a-cellular-uptake-and-distribution-study
#1
Nan Zheng, Bin Lian, Wenwen Du, Guobing Xu, Jiafu Ji
Paclitaxel-loaded polymeric micelles (PTX-PM) are commonly used as tumor-targeted nanocarriers and display outstanding antitumor features in clinic, but its accumulation and distribution in vitro are lack of investigation. It is probably due to the complex micellar system and its low concentration at the cellular or subcellular levels. In this study, we developed an improved extraction method, which was a combination of mechanical disruption and liquid-liquid extraction (LLE), to extract the total PTX from micelles in the cell lysate and subcellular compartments...
December 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29222826/application-of-ultra-performance-liquid-chromatography-coupled-with-quadrupole-time-of-flight-mass-spectrometry-in-identification-of-three-isoflavone-glycosides-and-their-corresponding-metabolites
#2
Xiafen Xu, Xinhui Li, Xianrui Liang
RATIONALE: Metabolites of isoflavones raised great attention due to the potential bioactivities in recent years. However, the complex constituents of metabolic system and low level of metabolites made it difficult to analyze. Mass spectrometry (MS) method was applied in our metabolites identification and fragmentation pathways study due to the advantages of rapidity, sensitivity, and low level of sample consumption. METHODS: Three isoflavone glycosides and their metabolites were identified with using ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC/QTOF-MS)...
December 8, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29222715/clinical-validation-study-of-dried-blood-spot-for-determining-everolimus-concentration-in-patients-with-cancer
#3
A E C A B Willemsen, L M Knapen, Y M de Beer, R J M Brüggemann, S Croes, C M L van Herpen, N P van Erp
PURPOSE: Everolimus treatment is seriously hampered by its toxicity profile. As a relationship between everolimus exposure and effectiveness and toxicity has been established, early and ongoing concentration measurement can be key to individualize the dose and optimize treatment outcomes. Dried blood spot (DBS) facilitates sampling at a patients' home and thereby eases dose individualization. The aim of this study is to determine the agreement and predictive performance of DBS compared to whole blood (WB) to measure everolimus concentrations in cancer patients...
December 8, 2017: European Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/29222475/suitability-of-various-chromatographic-and-spectroscopic-techniques-for-analysis-and-kinetic-degradation-study-of-trelagliptin
#4
Wafaa A Zaghary, Shereen Mowaka, Mostafa A Hassan, Bassam M Ayoub
Multifaceted comparative analytical methods for trelagliptin (TRL) were investigated, applied to ZAFATEK tablets and HPLC-UV was selected for a degradation kinetic study. UPLC-MS/MS (Method I), UPLC-UV (Method II), HPLC-UV (Method III), UHPLC-UV (Method IV) and direct UV (Method V) methods were developed. Methods (I-V) showed satisfactory results using TRL concentration ranges of 50-800 ng/mL, 2.5-80 μg/mL, 5-100 μg/mL, 5-100 μg/mL and 5-50 μg/mL, respectively. Multiple Reaction Monitoring (MRM) of the transition pairs of m/z 358...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29220776/analysis-of-metabolites-of-organophosphate-and-pyrethroid-pesticides-in-human-urine-from-urban-and-agricultural-populations-catalonia-and-galicia
#5
Mercè Garí, Yolanda González-Quinteiro, Natalia Bravo, Joan O Grimalt
Isotope dilution solid phase extraction UPLC-MS/MS has been used to develop a robust and rapid methodology for the determination of eight specific metabolites of organophosphate and pyrethroid pesticides in human urine. The use of methanol:acetone (25:75v/v) affords an improvement in extraction efficiency in comparison to these individual solvents. The use of synthetic urine improves selectivity and limits of detection for the calibration straight lines. The method provides detection limits of 14-69pg/ml and 18-19pg/ml for the organophosphate and pyrethroid metabolites, respectively...
December 5, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29218946/-metabolism-of-inula-cappa-extract-by-rat-intestinal-bacteria-in-vitro
#6
Zi-Peng Gong, Jing-Yu Hou, Mei Li, Lin-Lin Wu, Ting-Ting Chen, Yong-Jun Li, Ai-Min Wang, Yan-Yu Lan, Yong-Lin Wang
To investigate the metabolism of major components in Inula cappa by rat intestinal bacteria in vitro. I. cappa extract was incubated for 24 h with rat intestinal bacteria under anaerobic environment. After the samples were precipitated by n-butanol, UPLC-Q-TOF-MS/MS was applied for the qualitative analysis of the metabolites, combined with data software such as Metabolite Tools, Data Analysis and so on. The potential metabolites in rat intestinal bacteria were analyzed by comparing the total ion current of the test samples and blank samples and analyzing the quasi-molecular ion and fragment ion of all chromatograms...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29218942/-analysis-of-alcohol-extract-and-water-extract-of-polygonum-capitatum-by-uplc-q-tof-ms
#7
Wen Zhou, Li-Yan Zhang, Li-Min Xie, Li-Fang Qiu, Jing-Rong Wang, Si-Bu Ma, Zhi-Hong Jiang, Jing-Wen Tang
In this study, we used Ultra Performance Liquid Chromatography-Time-of-Flight Mass Spectrometry(UPLC-TOF-MS)to identify the chemical constituents in both ethanol and water extract of Polygonum capitatum. A Waters ACQUITY UPLC BEH C₁₈ column(2.1 mm×100 mm,1.7 μm) was used for separation. The mobile phase was consisted of(A) 0.10% formic acid in water and(B)0.10% formic acid in acetonitrile, and the flow rate was 0.35 mL•min⁻¹. ESI source in negative ion mode was used for MS detection. Structural identification was carried out according to the accurate mass and matching with database...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29218939/-evaluate-effects-of-different-drying-methods-on-quality-of-tianshu-capsule-on-substance-basis
#8
Meng-Ting Zhang, Yu-An Bi, Xin Zhang, Gui-Hong Xu, Wen-Zhe Huang, Zhen-Zhong Wang, Wei Xiao
To investigate the effect of hot air circulation drying and spray drying on the quality of Tianshu capsule from the view point of chemical compositions. UPLC-DAD was used to establish the fingerprint of Tianshu capsules for the first time, and the main chemical constituents were identified by UPLC-Q-TOF-MS/MS. A total of 62 compounds were identified in this method, 21 of which were reported in Tianshu capsules for the first time. The results showed that there were no significant differences in the identification of the chemical constituents types between these two methods, but the contents of some constituents were different...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29218938/-analysis-of-chemical-markers-in-sulfur-fumigated-anemarrhenae-rhizoma
#9
Wei Zheng, Fang-Xu Wang, Xin-Guang Sun, Li-Ping Kang, Yang Zhao, Jie Zhang, Bai-Ping Ma
Sulfur-containing Anemarrhenae Rhizoma decoction pieces were prepared by using sulfur-fumigating procedure. The difference components before and after sulfur fumigation in Anemarrhenae Rhizoma were analyzed and on-line identified by UPLC-Q-TOF-MSE combined with UNIFI informatics platform, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) respectively. As a result, 16 major differences components were identified, and among them, 9 components were mainly from sulfur-fumigated samples...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29218927/-qualitative-and-quantitative-analyses-of-primary-saponins-in-paris-forrestii
#10
Yuan-Yuan Huang, Li-Ping Kang, Hua-Sheng Peng, Da-Hui Liu, Qing-Xiu Hao, Jia-Liu Zhao, Min Chen, Lu-Qi Huang
In order to study whether Paris forrestii could be developed as a substitute of Paridis Rhizome, chemical compositions of P. forrestii and P. polyphylla var. yunnanensis were investigated by UPLC-Q-TOF MS. In addition, the contents of eight primary steroidal saponins in 77 batches of P. forrestii samples from different habitats were simultaneously determined by HPLC-UV. The results showed that P. forrestii and P. polyphylla var. yunnanensis have similar chemical compositions, and all 22 major common peaks were identified as steroid derivatives...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29218926/-determination-of-eight-steroidal-saponins-in-15-kinds-of-genus-paris
#11
Yuan-Yuan Huang, Da-Hui Liu, Hua-Sheng Peng, Qing-Xiu Hao, Mei-Lan Chen, A-Zhong Zhang, Min Chen, Li-Ping Kang, Lu-Qi Huang
The study was established an HPLC method for simultaneous determination of 8 steroidal saponins (polyphyllins Ⅶ, H, Ⅵ, Ⅱ, Ⅰ, and Ⅴ, dioscin, and gracillin) in Paridis Rhizoma, and made an evaluation by determining steroidal saponins in 15 kinds of genus Paris. The analysis was performed on a Waters Acquity H-ClassTM UPLC ultrafine liquid chromatography system coupled with a PDA detector. The chromatographic separation was achieved through a CAPCELL PAK ADME (4.6 mm× 250 mm, 5 μm) column and the optimal mobile phase consisted of acetonitrile and water...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29218012/anti-tumor-effect-of-rhaponticum-uniflorum-ethyl-acetate-extract-by-regulation-of-peroxiredoxin1-and-epithelial-to-mesenchymal-transition-in-oral-cancer
#12
Hui Chen, Chunxiao Wang, Moci Qi, Lihua Ge, Zhenchuan Tian, Jinhua Li, Min Zhang, Min Wang, Linfang Huang, Xiaofei Tang
Objective: To explore whether Rhaponticum uniflorum (R. uniflorum) had anti-tumor effects in oral cancer and investigate the molecular mechanisms involved in these anti-tumor effects. Methods: Chemical compositions of R. uniflorum ethyl acetate (RUEA) extracts were detected by ultra-performance liquid chromatography-Q/time-of-flight mass spectrometry (UPLC-Q/TOF-MS), followed by pharmacology-based network prediction analysis. The effects of RUEA extracts on proliferation, apoptosis, migration, and invasion ability of human oral squamous cell carcinoma (OSCC) cell line SCC15 were evaluated by CCK8 assay, Annexin V- fluorescein isothiocyanate/propidium iodide staining, wound healing assay, and Matrigel invasion assay, respectively...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29217496/protective-action-of-crtaeva-nurvala-buch-ham-extracts-against-renal-ischaemia-reperfusion-injury-in-rats-via-antioxidant-and-anti-inflammatory-activities
#13
Mouchira Aly Choucry, Mohammed N A Khalil, Sally A El Awdan
ETHNOPHARMACOLOGICAL RELEVANCE: Crateva nurvala stem bark is commonly used in Ayruveda in treatment of many renal injuries, e.g., urinary lithiasis, diuretic and nephroprotective. However, its protective effect against renal ischaemia/reperfusion, the major cause of acute kidnea injury, has never been studied. Moreover, no comprehensive chemical profiling of its extracts was recorded. AIM OF THE STUDY: Assessment of the protective effect of both extracts against renal ischaemia/reperfusion and elucidation of the possible mechanism of action...
December 4, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29217478/identification-of-a-gene-encoding-a-flavoprotein-involved-in-bile-acid-metabolism-by-the-human-gut-bacterium-clostridium-scindens-atcc-35704
#14
Spencer C Harris, Saravanan Devendran, João M P Alves, Sean M Mythen, Phillip B Hylemon, Jason M Ridlon
BACKGROUND: The multi-step bile acid 7α-dehydroxylating pathway by which a few species of Clostridium convert host primary bile acids to toxic secondary bile acids is of great importance to gut microbiome structure and host physiology and disease. While genes in the oxidative arm of the 7α-dehydroxylating pathway have been identified, genes in the reductive arm of the pathway are still obscure. METHODS: We identified a candidate flavoprotein-encoding gene predicted to metabolize steroids...
December 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29217223/temporal-variability-of-global-dna-methylation-and-hydroxymethylation-in-buccal-cells-of-healthy-adults-association-with-air-pollution
#15
Siemon De Nys, Radu-Corneliu Duca, Tim Nawrot, Peter Hoet, Bart Van Meerbeek, Kirsten L Van Landuyt, Lode Godderis
BACKGROUND: Epigenetic changes, such as DNA methylation, are observed in response to environmental exposure and in the development of several chronic diseases. Consequently, DNA methylation alterations might serve as indicators of early effects. In this context, the aim of this study was to assess the temporal variability of global DNA methylation and hydroxymethylation levels in buccal cells from healthy adult volunteers. METHODS: Global DNA methylation (%5mdC) and hydroxymethylation (%5hmdC) levels in human buccal cells, collected from 26 healthy adults at different time points, were quantified by UPLC-MS/MS...
December 4, 2017: Environment International
https://www.readbyqxmd.com/read/29215203/analysis-of-ketamine-and-xylazine-in-complex-matrices-using-two-dimensional-2d-lc-ms-ms
#16
Malorie Mella, Brenda Schweitzer, Sabra Botch-Jones, Claude Mallet
RATIONALE: Analyzing tissue samples is routinely performed when liquid biological samples are not available for replicate analysis. Preparing complex matrices, such as tissue, for analysis can be time-consuming. Traditional sample preparation methods typically begin with homogenization followed by a sample clean up step such as liquid-liquid or solid phase extraction. Samples are typically eluted, evaporated and reconstituted prior to instrumental analysis. The aim of this project was to evaluate multi-dimensional chromatography's utility in reducing the amount of time from sample acquisition to analysis...
December 7, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29213243/mechanism-of-chinese-medicine-herbs-effects-on-chronic-heart-failure-based-on-metabolic-profiling
#17
Kuo Gao, Huihui Zhao, Jian Gao, Binyu Wen, Caixia Jia, Zhiyong Wang, Feilong Zhang, Jinping Wang, Hua Xie, Juan Wang, Wei Wang, Jianxin Chen
Chronic heart failure (CHF) is a major public health problem in huge population worldwide. The detailed understanding of CHF mechanism is still limited. Zheng (syndrome) is the criterion of diagnosis and therapeutic in Traditional Chinese Medicine (TCM). Syndrome prediction may be a better approach for understanding of CHF mechanism basis and its treatment. The authors studied disturbed metabolic biomarkers to construct a predicting mode to assess the diagnostic value of different syndrome of CHF and explore the Chinese herbal medicine (CHM) efficacy on CHF patients...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29212895/functional-metabolomics-characterizes-a-key-role-for-n-acetyl-neuraminic-acid-in-coronary-artery-diseases
#18
Lei Zhang, Ting-Ting Wei, Yong Li, Jing Li, Yong Fan, Feng-Qing Huang, Yuan-Yuan Cai, Gaoxiang Ma, Jin-Feng Liu, Qian-Qian Chen, Shi-Lei Wang, Honglin Li, Raphael N Alolga, Baolin Liu, Dong-Sheng Zhao, Jian-Hua Shen, Xiang-Ming Wang, Wei Zhu, Ping Li, Lian-Wen Qi
Background -As new biomarkers of coronary artery diseases (CAD) emerge via metabolomics, the underlying functional mechanisms remain to be elucidated. Functional metabolomics aims to translate metabolomics-derived biomarkers to disease mechanisms. Methods -A cohort of 2324 patients who underwent coronary angiography from 4 independent centers was studied. A combination of ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) in the negative ion mode was employed for untargeted analysis of metabolites in plasma...
December 6, 2017: Circulation
https://www.readbyqxmd.com/read/29212488/identification-of-the-lipid-biomarkers-from-plasma-in-idiopathic-pulmonary-fibrosis-by-lipidomics
#19
Feng Yan, Zhensong Wen, Rui Wang, Wenling Luo, Yufeng Du, Wenjun Wang, Xianyang Chen
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is an irreversible interstitial pulmonary disease featured by high mortality, chronic and progressive course, and poor prognosis with unclear etiology. Currently, more studies have been focusing on identifying biomarkers to predict the progression of IPF, such as genes, proteins, and lipids. Lipids comprise diverse classes of molecules and play a critical role in cellular energy storage, structure, and signaling. The role of lipids in respiratory diseases, including cystic fibrosis, asthma and chronic obstructive pulmonary disease (COPD) has been investigated intensely in the recent years...
December 6, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/29211893/aldosterone-is-not-associated-with-metabolic-and-microvascular-insulin-sensitivity-in-abdominally-obese-men
#20
Monica T J Schütten, Yvo H A M Kusters, Alfons J H M Houben, Jean L J M Scheijen, Marjo P H van de Waarenburg, Casper G Schalkwijk, Peter J Joris, Jogchum Plat, Ronald P Mensink, Peter W de Leeuw, Coen D A Stehouwer
Context: Impaired insulin-mediated muscle microvascular recruitment (IMMR) in obese individuals may add to the development of metabolic insulin resistance and hypertension. Increased aldosterone levels have been linked to these obesity-related complications in severely to morbidly obese individuals, and have been found to interfere with microvascular function in experimental studies. Objectives: To investigate whether aldosterone levels are related to IMMR, insulin sensitivity and blood pressure in lean and moderately abdominally obese men, and to study the effect of weight loss on these variables...
December 1, 2017: Journal of Clinical Endocrinology and Metabolism
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