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https://www.readbyqxmd.com/read/28917164/uplc-ms-ms-assay-of-riluzole-in-human-plasma-and-cerebrospinal-fluid-csf-application-in-samples-from-spinal-cord-injured-patients
#1
Mahua Sarkar, Robert G Grossman, Elizabeth G Toups, Diana S-L Chow
In the present study, a sensitive and robust LC-MS/MS method has been developed and validated for the quantification of riluzole in human plasma and cerebrospinal fluid (CSF) in clinical samples from patients with spinal cord injury (SCI). Riluzole and its labeled internal standard (IS) were isolated from plasma and CSF by liquid-liquid extraction using ethyl acetate. Riluzole (m/z 235→166) and IS (m/z 238→169) were detected by electrospray ionization (ESI) using multiple reaction monitoring (MRM) in a positive mode...
September 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28916568/perspective-the-paradox-in-dietary-advanced-glycation-end-products-research-the-source-of-the-serum-and-urinary-advanced-glycation-end-products-is-the-intestines-not-the-food
#2
Luanne R DeChristopher
Inconsistent research results have impeded our understanding of the degree to which dietary advanced glycation end products (dAGEs) contribute to chronic disease. Early research suggested that Western-style fast foods, including grilled and broiled meats and French fries, contain high levels of proinflammatory advanced glycation end products (AGEs). However, recent studies with state-of-the-art ultraperformance LC-tandem mass spectrometry (UPLC-MS) found that there is no evidence that these foods have elevated levels of dAGEs relative to other foods...
September 2017: Advances in Nutrition
https://www.readbyqxmd.com/read/28916333/human-plasma-metabolomics-study-across-all-stages-of-age-related-macular-degeneration-identifies-potential-lipid%C3%A2-biomarkers
#3
Inês Laíns, Rachel S Kelly, John B Miller, Rufino Silva, Demetrios G Vavvas, Ivana K Kim, Joaquim N Murta, Jessica Lasky-Su, Joan W Miller, Deeba Husain
PURPOSE: To characterize the plasma metabolomic profile of patients with age-related macular degeneration (AMD) using mass spectrometry (MS). DESIGN: Cross-sectional observational study. PARTICIPANTS: We prospectively recruited participants with a diagnosis of AMD and a control group (>50 years of age) without any vitreoretinal disease. METHODS: All participants underwent color fundus photography, used for AMD diagnosis and staging, according to the Age-Related Eye Disease Study classification scheme...
August 31, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28916102/identification-of-novel-non-ionic-cationic-zwitterionic-and-anionic-polyfluoroalkyl-substances-using-uplc-tof-ms-e-high-resolution-parent-ion-search
#4
Feng Xiao, Svetlana A Golovko, Mikhail Y Golovko
Poly- and perfluoroalkyl substances (poly- and per-PFASs) are a large group of organic compounds that have become the target of investigation due to their widespread occurrence in the environment and biota, coupled with their known or suspected impacts on human health. Recent studies have shown that a significant portion of poly-PFASs remain unidentified. This study presents a time-of-flight mass spectrometry approach based on continuously interleaving scans at low and high collision energies (ToF-MS(E)) for the rapid identification and characterization of unknown PFASs...
October 2, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28915534/revealing-anti-inflammation-mechanism-of-water-extract-and-oil-of-forsythiae-fructus-on-carrageenan-induced-edema-rats-by-serum-metabolomics
#5
An Yuan, Lihong Gong, Lin Luo, Jue Dang, Xiaohong Gong, Mengjie Zhao, Yan Li, Yunxia Li, Cheng Peng
Forsythiae Fructus is an important Chinese medicine which shows a significant effect against inflammation. This study aimed to investigate the preventive anti-inflammation mechanism of Forsythiae Fructus by serum metabolomics strategy and compare the difference of the metabolism pathways between Forsythia extract and Forsythia oil in rat. Four groups (control group, model group, Forsythia extract group and Forsythia oil group) were orally administered 10mL/kg 0.5% Tween 80 solution, 10mL/kg 0.5% Tween 80 solution, 5g/kg Forsythia extract and 0...
September 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28912714/anti-inflammatory-and-analgesic-effects-of-polygonum-orientale-l-extracts
#6
Kai-Jun Gou, Rui Zeng, Yan Dong, Qi-Qi Hu, Huang-Wan-Yin Hu, Katherine G Maffucci, Qi-Ling Dou, Qing-Bo Yang, Xu-Hua Qin, Yan Qu
Background and Purpose:Polygonum orientale L. (family: Polygonaceae), named Hongcao in China, is a Traditional Chinese Medicinal and has long been used for rheumatic arthralgia and rheumatoid arthritis. However, no pharmacological and mechanism study to confirm these clinic effects have been published. In this investigation, the anti-inflammatory, analgesic effects and representative active ingredient compounds of P. orientale have been studied. Methods: Dried small pieces of the stems and leaves of P. orientale were decocted with water and partitioned successively to obtain ethyl acetate and ethyl ether extract of P...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28912268/structural-and-functional-diversity-in-listeria-cell-wall-teichoic-acids
#7
Yang Shen, Samy Boulos, Eric Sumrall, Benjamin Gerber, Alicia Julian-Rodero, Marcel R Eugster, Lars Fieseler, Laura Nyström, Marc-Olivier Ebert, Martin J Loessner
Wall teichoic acids (WTAs) are the most abundant glycopolymers found on the cell wall of many Gram-positive bacteria, whose diverse surface structures play key roles in multiple biological processes. Despite recent technological advances in glycan analysis, structural elucidation of WTAs remains challenging due to their complex nature. Here, we employed a combination of UPLC-MS/MS and NMR to determine the structural complexity of WTAs from Listeria species. We unveiled more than 10 different types of WTA polymers that vary in their linkage and repeating units...
September 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28911654/extraction-optimization-of-gallic-acid-catechin-%C3%A2-procyanidin-b2-epicatechin-epigallocatechin-gallate-and-epicatechin-gallate-their-simultaneous-identification-and-quantification-in-saraca-asoca
#8
Tushar Dhanani, Raghuraj Singh, Satyanshu Kumar
The objective of the present investigation was to optimize extraction conditions for maximum recovery of bioactive phenolics from different parts of Saraca asoca. Extraction recovery was optimized using a mixture of methanol and water in different proportions. For identification and quantification of six analytes, a rapid reversed phase ultra-performance liquid chromatography (UPLC) photo diode array detection method was developed. UPLC separation was achieved in a gradient elution mode on a C18 column with acetonitrile and aqueous phosphoric acid (0...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28910408/effects-of-tumour-necrosis-factor-%C3%AE-upon-the-metabolism-of-the-endocannabinoid-anandamide-in-prostate-cancer-cells
#9
Jessica Karlsson, Sandra Gouveia-Figueira, Mireille Alhouayek, Christopher J Fowler
Tumour necrosis factor α (TNFα) is involved in the pathogenesis of prostate cancer, a disease where disturbances in the endocannabinoid system are seen. In the present study we have investigated whether treatment of DU145 human prostate cancer cells affects anandamide (AEA) catabolic pathways. Additionally, we have investigated whether cyclooxygenase-2 (COX-2) can regulate the uptake of AEA into cells. Levels of AEA synthetic and catabolic enzymes were determined by qPCR. AEA uptake and hydrolysis in DU145 and RAW264...
2017: PloS One
https://www.readbyqxmd.com/read/28906164/comparative-pharmacokinetics-study-of-anastrozole-after-single-administration-and-combination-with-celecoxib
#10
Xiaoyang Sun, Mingming Lv, Binjie Wang, Simin Zhao, Ping Guo, Shixiao Wang, Liqiang Gu, Xiaohui Chen
1. There are numerous investigations demonstrating that the cyclooxygenase-2 (COX-2) inhibitors might enhance the efficiency of anastrozole in breast cancer. Hence, this study was conducted to investigate the comparative pharmacokinetics of anastrozole after single administration and combination with celecoxib. 2. A simple protein precipitation procedure was adopted for the sample preparation with satisfactory extraction recovery for both anastrozole and the internal standard, and then anastrozole was separated and analysed on an ACQUITY BEH UPLC C18 column (50 × 2...
September 14, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/28906007/intergrated-metabonomic-study-of-the-effects-of-guizhi-fuling-capsule-intervention-on-primary-dysmenorrheal-using-rp-uplc-ms-complementary-with-hilic-uplc-ms-technique
#11
Lang Lang, Zhaorui Meng, Lan Sun, Wei Xiao, Longshan Zhao, Zhili Xiong
Guizhi Fuling capsule (GFC), developed from the traditional Chinese prescription of Guizhi Fuling Wan, has been commonly used for the treatment of primary dysmenorrheal (PD). However, the intervention effective mechanism in vivo has not been well elucidated. In this study, an integrated plasma metabonomic strategy based on RP-UPLC-MS coupled with HILIC-UPLC-MS technique has been developed to investigate the global therapeutic effects and intervention mechanisms of Guizhi Fuling capsule (GFC) on dysmenorrhea rats induced by oxytocin...
September 14, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28906001/overcoming-interference-of-plasma-phospholipids-using-hybridspe-for-the-determination-of-trimetazidine-by-uplc-ms-ms
#12
Pravin G Vanol, Manish Yadav, Mallika Sanyal, Priyanka A Shah, Pranav S Shrivastav
An improved, precise and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the quantification of trimetazidine, using trimetazidine-d8 as the internal standard (IS). Interference due to plasma phospholipids during sample preparation was overcome by using hybrid solid phase extraction-phospholipid ultra cartridge. The mean extraction recovery of trimetazidine (98.66 %) and trimetazidine-d8 (97.63 %) from spiked plasma was consistent and reproducible...
September 14, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28903089/simultaneous-determination-of-pentoxifylline-metabolites-m1-lisofylline-m4-and-m5-and-caffeine-in-plasma-and-dried-blood-spots-for-pharmacokinetic-studies-in-preterm-infants-and-neonates
#13
Madhu Page-Sharp, Tobias Strunk, Sam Salman, Julie Hibbert, Sanjay K Patole, Laurens Manning, Kevin T Batty
Advances in bioanalytical methods are facilitating micro-volume and dried blood spot (DBS) analysis of drugs in biological matrices for pharmacokinetic studies in children and neonates. We sought to develop a UPLC-MS/MS assay for simultaneous measurement of caffeine, pentoxifylline (PTX) and three metabolites of PTX in both plasma and DBS. Caffeine, PTX, the metabolites M1 (lisofylline), M4 and M5, and the internal standards (caffeine-d9 and PTX-d6) were separated using a Waters Aquity T3 UPLC C18 column and gradient mobile phase (water-methanol-formic acid)...
August 26, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28901489/uplc%C3%A2-qtofms%C3%A2-based-metabolomic-analysis-of-the-serum-of-hypoxic-preconditioning-mice
#14
Jie Liu, Gang Zhan, Dewei Chen, Jian Chen, Zhi-Bin Yuan, Er-Long Zhang, Yi-Xing Gao, Gang Xu, Bing-Da Sun, Wenting Liao, Yu-Qi Gao
Hypoxic preconditioning (HPC) is well‑known to exert a protective effect against hypoxic injury; however, the underlying molecular mechanism remains unclear. The present study utilized a serum metabolomics approach to detect the alterations associated with HPC. In the present study, an animal model of HPC was established by exposing adult BALB/c mice to acute repetitive hypoxia four times. The serum samples were collected by orbital blood sampling. Metabolite profiling was performed using ultra‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry (UPLC‑QTOFMS), in conjunction with univariate and multivariate statistical analyses...
September 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28895795/glycan-characterization-of-the-nist-rm-monoclonal-antibody-using-a-total-analytical-solution-from-sample-preparation-to-data-analysis
#15
Mark Hilliard, William R Alley, Ciara A McManus, Ying Qing Yu, Sinead Hallinan, John Gebler, Pauline M Rudd
Glycosylation is an important attribute of biopharmaceutical products to monitor from development through production. However, glycosylation analysis has traditionally been a time-consuming process with long sample preparation protocols and manual interpretation of the data. To address the challenges associated with glycan analysis, we developed a streamlined analytical solution that covers the entire process from sample preparation to data analysis. In this communication, we describe the complete analytical solution that begins with a simplified and fast N-linked glycan sample preparation protocol that can be completed in less than 1 hr...
September 12, 2017: MAbs
https://www.readbyqxmd.com/read/28895216/metabolic-alterations-in-two-cirsium-species-identified-at-distinct-phenological-stages-using-uplc-qtof-ms
#16
Min-Sun Kim, Miso Nam, Geum-Sook Hwang
INTRODUCTION: Cirsium chanroenicum and C. setidens are commonly used both in traditional folk medicine and as a food source. The quality of different species of Cirsium at different harvest times is a function of their metabolite composition, which is determined by the phenological stage. OBJECTIVE: We sought to determine the differences in the metabolite composition of two species of Cirsium during different phenological stages using ultra-performance liquid chromatography (UPLC) quadrupole time-of-flight (QTOF) mass spectrometry (MS)...
September 12, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28892155/pharmacokinetics-of-tildipirosin-in-beagle-dogs
#17
J Wang, T Zhao, X Sun, Y Liu, J Zhu, S Zhang, X Cao
The objective of this study was to investigate the pharmacokinetic profile of tildipirosin (TD) in 24 beagle dogs following intravenous (i.v.) and intramuscular (i.m.) administration, respectively, at 2, 4, and 6 mg/kg. Plasma samples at certain time points (0-14 days) were collected, and the concentrations of drug were quantified by UPLC-MS/MS. Plasma concentration-time data and relevant parameters were described by noncompartmental through WinNonlin 6.4 software. After single i.m. injection at 2, 4, and 6 mg/kg body weight, mean maximum concentration (Cmax ) was 412...
September 11, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28892000/development-of-a-matrix-solid-phase-dispersion-extraction-combined-with-uplc-q-tof-ms-for-determination-of-phenolics-and-terpenoids-from-the-euphorbia-fischeriana
#18
Wenjing Li, Yu Lin, Yuchun Wang, Bo Hong
A method based on a simplified extraction by matrix solid phase dispersion (MSPD) followed by ultra-performance liquid chromatography coupled with the quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS) determination is validated for analysis of two phenolics and three terpenoids in Euphorbia fischeriana. The optimized experimental parameters of MSPD including dispersing sorbent (silica gel), ratio of sample to dispersing sorbent (1:2), elution solvent (water-ethanol: 30-70) and volume of the elution solvent (10 mL) were examined and set down...
September 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28890760/insecticide-residues-on-poultry-manures-field-efficacy-test-on-selected-insecticides-in-managing-musca-domestica-population
#19
Song-Quan Ong, Abdul Hafiz Ab Majid, Hamdan Ahmad
In this study, bifenthrin (Maxxthor SC, Ensystex Australasia Pty Ltd), imidacloprid (Prothor SC, Ensystex Australasia Pty Ltd) and fipronil (Regent(®)50SC, Bayer) were applied on the natural infest manures according to the manufacturer rate during a broiler breeding cycle. Solvent direct-immersion extraction (SDIE) was used in detecting the target compound and later, quantification of the insecticide residues in field condition was investigated. The samples were prior cleaned up by solid-phase extraction (SPE) and analysed by Ultra-Performance Liquid Chromatography (UPLC) - photodiode array (PDA) system...
July 2017: Tropical Life Sciences Research
https://www.readbyqxmd.com/read/28889287/spectrophotometric-and-mass-spectroscopic-methods-for-the-quantification-and-kinetic-evaluation-of-in-vitro-c-di-gmp-synthesis
#20
Geoffrey B Severin, Christopher M Waters
The expression and activity of diguanylate cyclase (DGC) and phosphodiesterase (PDE) enzymes are responsible for modulating and maintaining the intracellular concentration of the bacterial second messenger cyclic diguanosine-monophosphate (c-di-GMP). Here, we describe an in vitro method for the spectrophotometric detection and quantification of DGC catalyzed c-di-GMP synthesis through adaptation of the EnzChek(®) Pyrophosphate Assay Kit. We also outline a method for the quantification of c-di-GMP produced in this in vitro reaction using Ultra-Performance Liquid Chromatography tandem Mass Spectrometry (UPLC-MS/MS)...
2017: Methods in Molecular Biology
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