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https://www.readbyqxmd.com/read/28432922/restricted-access-magnetic-core-mesoporous-shell-microspheres-with-c8-modified-interior-pore-walls-for-the-identification-of-20-s-protopanaxadiol-metabolites-in-rat-plasma-using-uplc-q-tof-ms-ms
#1
Jin Ling, Yingjia Yu, Jianan Feng, Changjiang Xu, Jiebing Jiang, Liping Wang, Jiakun Long, Yan Li, Gengli Duan
In the present study, a novel sample preparation method based on magnetic core-mesoporous shell microspheres with C8-modified interior pore walls (C8-Fe3O4@mSiO2) was established for the identification of 20(S)-protopanaxadiol (PPD) metabolites in rat plasma by UPLC-Q-TOF-MS/MS analysis. C8-Fe3O4@mSiO2 allowed selective extraction of PPD metabolites from rat plasma by excluding macromolecules in the plasma owing to size exclusion effect. Five extraction conditions including the amount of C8-Fe3O4@mSiO2 microspheres used, extraction time, elution solvents, elution volume, and elution time were investigated and optimized...
April 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28432441/direct-quantification-of-lipopeptide-biosurfactants-in-biological-samples-via-hplc-and-uplc-ms-requires-sample-modification-with-an-organic-solvent
#2
Piotr Biniarz, Marcin Łukaszewicz
The rapid and accurate quantification of biosurfactants in biological samples is challenging. In contrast to the orcinol method for rhamnolipids, no simple biochemical method is available for the rapid quantification of lipopeptides. Various liquid chromatography (LC) methods are promising tools for relatively fast and exact quantification of lipopeptides. Here, we report strategies for the quantification of the lipopeptides pseudofactin and surfactin in bacterial cultures using different high- (HPLC) and ultra-performance liquid chromatography (UPLC) systems...
April 22, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28427917/application-of-the-isoniazid-assay-in-dried-blood-spots-using-the-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#3
Kyunghoon Lee, Sun-Hee Jun, Moon-Suk Choi, Sang Hoon Song, Jong Sun Park, Jae Ho Lee, Kyoung Un Park, Junghan Song
OBJECTIVES: Therapeutic drug monitoring (TDM) of anti-tuberculosis (TB) drugs is important for proper treatment of TB. Dried blood spots (DBSs) are widely used for TDM because of their several advantages. Rifampicin and pyrazinamide assays with DBSs have already been developed. However, isoniazid (INH) assay for capillary DBSs have not been reported because of INH instability. We developed an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for measuring INH concentrations in venous and capillary DBSs...
April 17, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28425490/urinary-and-fecal-metabonomics-study-of-the-protective-effect-of-chaihu-shu-gan-san-on-antibiotic-induced-gut-microbiota-dysbiosis-in-rats
#4
Meng Yu, Hong-Mei Jia, Chao Zhou, Yong Yang, Li-Li Sun, Zhong-Mei Zou
Accumulating evidence suggests that the gut microbiota dysbiosis and their host metabolic phenotype alteration is an important factor in human disease development. A traditional Chinese herbal formula, Chaihu-Shu-Gan-San (CSGS), has been effectively used in the treatment of various gastrointestinal (GI) disorders. The present study was carried out to investigate whether CSGS modulates the host metabolic phenotype under the condition of gut microbiota dysbiosis. The metabonomics studies of biochemical changes in urine and feces of antibiotic-induced gut microbiota dysbiosis rats after treatment with CSGS were performed using UPLC-Q-TOF/MS...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425100/mass-spectrometry-analysis-and-in-silico-prediction-of-allergenicity-of-peptides-in-tryptic-hydrolysates-of-the-proteins-from-ruditapes-philippinarum
#5
Yue Yu, Hongwei Liu, Maolin Tu, Meiling Qiao, Zhenyu Wang, Ming Du
BACKGROUND: The Ruditapes philippinarum is nutrient-rich and widely-distributed, but little attentions had been paid on the identification and characterization of bioactive peptides in the bivalve. In this study, we evaluated the peptides of the Ruditapes philippinarum enzymolysised by trypsin using a combination of UPLC separation and a ESI-Q-TOF-MS/MS spectrometer, followed by data processing, sequence-similarity database searching. The potential allergenicity of the peptides was assessed in silico...
April 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28422181/antioxidant-synergistic-effects-of-osmanthus-fragrans-flowers-with-green-tea-and-their-major-contributed-antioxidant-compounds
#6
Shuqin Mao, Kaidi Wang, Yukun Lei, Shuting Yao, Baiyi Lu, Weisu Huang
The antioxidant synergistic effects of Osmanthus fragrans flowers with green tea were evaluated, and their major antioxidant compounds contributed to the total amount of synergy were determined. The antioxidant compounds in O. fragrans flowers with green tea were identified by LC-MS and quantified by UPLC-PDA. The synergistic antioxidant interactions between O. fragrans flowers with green tea and their antioxidant compounds were tested using the Prieto's model after the simulated digestion. The main antioxidant compounds in O...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28422148/chemical-and-bioactive-profiling-and-biological-activities-of-coral-fungi-from-northwestern-himalayas
#7
Sapan Kumar Sharma, Nandini Gautam
Ramaria Fr. and Clavaria L. are the two major genera of coral mushrooms within families Gomphaceae and Clavariaceae, respectively. Besides having important role in forest ecology, some species of these are reported to possess high nutraceutical and bioactive potential. There is a hidden diversity of coral mushrooms in Northwestern Himalayas. Present studies describe the detailed biochemical profiling and antioxidant, and antibacterial activities of twelve coral mushroom species. Biochemical profiling of nutrients and nutraceuticals was done with standard techniques and by using HPLC, UPLC and GC...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28421984/stability-indicating-uplc-method-for-the-estimation-of-nadifloxacin-terbinafine-hydrochloride-mometasone-furoate-methyl-paraben-and-propyl-paraben-in-topical-pharmaceutical-dosage-form
#8
Dileep M Bhosale, Anna Pratima G Nikalje
A novel and simple ultra-performance LC method was developed for the estimation of nadifloxacin (NAD), terbinafine hydrochloride (TBH), mometasone furoate (MMF), methyl paraben (MP), and propyl paraben (PP) in a topical pharmaceutical dosage formulation. The analysis was carried out on a Waters Acquity UPLC ethylene bridged hybrid C18 column (50 × 2.1 mm, 1.7 μm) with a flow rate of 0.4 mL/min in gradient mode at a wavelength of 255 nm. Elution of all components was achieved within 9 min. The retention times of MP, NAD, PP, TBH, and MMF were observed at 1...
April 18, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28421713/identification-isolation-and-synthesis-of-seven-novel-impurities-of-anti-diabetic-drug-repaglinide
#9
Prasad Kancherla, Srinivas Keesari, Pallavi Alegete, Mukkanti Khagga, Parthasarathi Das
Seven unknown impurities in Repaglinide bulk drug batches at below 0.1% (ranging from 0.05-0.10%) were detected by an ultra-performance liquid chromatographic (UPLC) method. These impurities were isolated from the crude sample of Repaglinide using preparative high performance liquid chromatography (prep-HPLC). Based on LC-ESI/MS study, the chemical structures of seven new impurities (8, 9, 10, 11, 13, 14 and 16) were presumed and characterized as 4-(cyanomethyl)-2-ethoxybenzoic acid (8), 4-(cyanomethyl)-2-ethoxy-N-(3-methyl-1-(2-(piperidin-1-yl)phenyl)butyl)benzamide (9), 4-(2-amino-2-oxoethyl)-2-ethoxy-N-(3-methyl-1-(2-(piperidin-1-yl)phenyl)butyl) benzamide (10) and 2-(3-ethoxy-4-((3-methyl-1-(2-(piperidin-1-yl)phenyl)butyl) carbamoyl) phenyl) acetic acid (11) and 4-(cyanomethyl)-N-cyclohexyl-2-ethoxybenzamide (13), 2-(4-(cyclohexylcarbamoyl)-3-ethoxyphenyl) acetic acid (14) and N-cyclohexyl-4-(2-(cyclohexylamino)-2-oxoethyl)-2-ethoxybenzamide (16)...
April 19, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28420061/metabolic-profiling-reveals-new-serum-biomarkers-of-lupus-nephritis
#10
J Li, X-W Xie, H Zhou, B Wang, M-J Zhang, F-Y Tang
Metabolomics has been applied to explore altered metabolite profiles in disease and identify unique metabolic signatures specific to certain pathologies. The aim of the current study is to characterize the metabolic profile of patients diagnosed with lupus nephritis (LN) and explore new insights into underlying disease processes. A metabolomic approach using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS) was developed in serum samples from 32 LN patients, 30 idiopathic nephrotic syndrome (INS) patients and 28 healthy controls (HCs)...
January 1, 2017: Lupus
https://www.readbyqxmd.com/read/28416338/separation-and-purification-of-polyphenols-from-red-wine-extracts-using-high-speed-counter-current-chromatography
#11
Yuanyuan Li, Lingxi Li, Yan Cui, Shuting Zhang, Baoshan Sun
Polyphenols are important compounds of red wine owing to their contribution to sensory properties and antioxidant activities. In this study, high-speed counter-current chromatography (HSCCC) coupled with semi-preparative HPLC was used for large-scale separation and purification of polyphenols from red wine extracts. With the solvent system of hexane-ethyl acetate-water (1-50-50), various oligomeric procyanidins including monomer catechin, epicatechin, dimers B1, B2; phenolic acids including coutaric acid, caftaric acid and other type of polyphenols were largely separated within 370min and most of these compounds presented high yields (0...
March 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28415783/mass-spectral-analysis-of-the-multikinase-inhibitor-bzg-and-its-metabolites-and-analysis-of-their-binding-to-vascular-endothelial-growth-factor-receptor-2
#12
Yan Lou, Wenqi Qiu, Zhe Wu, Qian Wang, Yunqing Qiu, Su Zeng
We previously showed that BZG is a novel multitarget kinase inhibitor, which inhibited hepatocellular carcinoma in vivo and in vitro. In the present study, we used ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) to characterize BZG and its metabolites generated in vivo. The probable metabolic mechanism was further confirmed by analysis of Phase I and Phase II metabolism in liver microsomes and with recombinant enzymes. In addition, the binding affinities of BZG metabolites to vascular endothelial growth factor receptor 2 (VEGFR2) were predicted using electronic high throughput screening (eHiTS)...
March 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28414972/an-lc-ms-ms-method-for-quantification-of-ac0010-a-novel-mutant-selective-epidermal-growth-factor-receptor-egfr-inhibitor-and-its-metabolites-in-human-plasma-and-the-application-to-a-pharmacokinetic-study
#13
Lu Wang, Xin Zheng, Weicong Wang, Pei Hu, Ji Jiang
AC0010 is an irreversible, mutant-selective EGFR inhibitor that effectively inhibits EGFR active and T790M resistance mutations in non-small cell lung cancer (NSCLC). A sensitive ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) method was developed and fully validated for determining AC0010 and its metabolites in human plasma. The samples were purified by solid-phase extraction (SPE) columns and separated on a BEH C18 column. Electrospray ionization (ESI) in positive ion mode and multiple reaction monitoring (MRM) were used to monitor the ion transitions of AC0010 (m/z 488→257) and its metabolites M1 (m/z 474→403), M2 (m/z 504→487), M4 (m/z 434→377), M7 (m/z 490→405), MII-1 (m/z 651→434) and MII-2 (m/z 609→434)...
March 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28414944/direct-and-indirect-electrochemical-reduction-prior-to-a-biological-treatment-for-dimetridazole-removal
#14
Melika Zaghdoudi, Florence Fourcade, Isabelle Soutrel, Didier Floner, Abdeltif Amrane, Hager Maghraoui-Meherzi, Florence Geneste
Two different electrochemical reduction processes for the removal of dimetridazole, a nitroimidazole-based antibiotic, were examined in this work. A direct electrochemical reduction was first carried out in a home-made flow cell in acidic medium at potentials chosen to minimize the formation of amino derivatives and then the formation of azo dimer. Analysis of the electrolyzed solution showed a total degradation of dimetridazole and the BOD5/COD ratio increased from 0.13 to 0.24. An indirect electrochemical reduction in the presence of titanocene dichloride ((C5H5)2TiCl2), which is used to reduce selectively nitro compounds, was then investigated to favour the formation of amino compounds over hydroxylamines and then to prevent the formation of azo and azoxy dimers...
April 9, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28414239/a-novel-sample-preparation-for-shotgun-proteomics-characterization-of-hcps-in-antibodies
#15
Lihua Huang, Ning Wang, Charles E Mitchell, Tammy J Brownlee, Steven R Maple, Michael R De Felippis
Residual host cell proteins (HCPs) in biopharmaceuticals derived from recombinant DNA technology can present potential safety risks to patients or compromise product stability. Thus, the downstream purification process is designed to demonstrate robust removal of these impurities. ELISA using polyclonal anti-HCP antibodies as reagents for capture, detection and quantitation purposes is most commonly used to monitor HCP removal during process development but this technique has limitations. More recently, LC-MS for residual HCP characterization has emerged as a powerful tool to support purification process development...
April 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28412875/amino-acid-and-acylcarnitine-profiles-in-perinatal-asphyxia-a-case-control-study
#16
Ola Galal El-Farghali, Mohamed Sami El-Chimi, Heba Salah El-Abd, Eman El-Desouky
OBJECTIVE: To estimate cord blood amino acid and acylcarnitine levels in term newborns exposed to perinatal asphyxia. MATERIALS AND METHODS: We studied 45 asphyxiated term newborns (cases) and 20 gestational age-matched healthy newborns (control). Sixteen cases developed HIE according to clinical scoring and amplitude-integrated electroencephalography. Asphyxiated cases were accordingly subdivided into: HIE group (n = 16) and Asphyxia group (n = 29). Amino acid and acylcarnitine levels were measured in cord blood dried spot samples from all newborns using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS)...
April 16, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28412859/determination-and-quantification-of-carotenoids-in-sea-sponges-raspaciona-aculeata-and-dictyonella-marsilii-present-in-the-ganzirri-lake-messina-italy
#17
Andrea Salvo, Daniele Giuffrida, Archimede Rotondo, Paolo De Pasquale, Giovanna Loredana La Torre, Giacomo Dugo
Considering the crucial role of carotenoids exploitable both as nutraceuticals and also as dyes in food industry, there are many efforts in seeking for new sources of these pigments, especially in the marine world. In this study, for the first time, we extracted carotenoids from sea sponges Raspaciona aculeata and Dictyonella marsilii taken from Ganzirri Lake Messina (Italy). The determination and quantification of carotenoids was made by UPLC-PDA-MS. Remarkable results concern renieratene content in R. aculeate found to be over 2570 ppm...
April 17, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28412848/proteomic-profile-of-serum-of-pregnant-women-carring-a-fetus-with-down-syndrome-using-nano-uplc-q-tof-ms-ms-technology
#18
Graciela Arelí López-Uriarte, Carlos Horacio Burciaga Flores, Víctor Manuel Torres de la Cruz, María Magdalena Medina Aguado, Viviana Maricela Gómez Puente, Liliana Nayeli Romero Gutiérrez, Laura Elia Martínez de Villarreal
INTRODUCTION: Prenatal diagnosis of Down syndrome (DS) is based on the calculated risk of maternal age, biochemical and ultrasonographic markers and recently by cfDNA. Differences in proteomic profiles may give an opportunity to find new biomarkers. OBJECTIVE: Characterize proteome of serum of mothers carrying DS fetus. MATERIAL AND METHODS: Blood serum samples of three groups of women were obtained, a) 10 non-pregnant, b) 10 pregnant with healthy fetus by ultrasound evaluation, c) 9 pregnant with DS fetus; Sample preparation was as follow: Albumin/IgG depletion, desalting, and trypsin digestion; the process was performed in nanoUPLC MS/MS...
April 16, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28412197/an-uplc-ms-ms-method-for-the-quantification-of-braf-inhibitors-vemurafenib-dabrafenib-and-mek-inhibitors-cobimetinib-trametinib-binimetinib-in-human-plasma-application-to-treated-melanoma-patients
#19
Marine Rousset, Karine Titier, Stephane Bouchet, Caroline Dutriaux, Anne Pham-Ledard, Sorilla Prey, Mireille Canal-Raffin, Mathieu Molimard
Targeted therapies for cancers are fast-growing therapies. For instance kinase inhibitors such as BRAF inhibitors (BRAFi) and MEK inhibitors (MEKi) are increasingly used to treat malignant melanoma. The metabolic profile of these drugs can result in great interindividual variability, justifying therapeutic drug monitoring (TDM). We describe a rapid and specific method for quantification of 2 BRAFi (vemurafenib, dabrafenib) and 3 MEKi (cobimetinib, trametinib and binimetinib). Chromatography was performed on a Waters Acquity-UPLC system with CORTECS C18+ column, under a gradient of 10% acetic acid in water/acetonitrile...
April 12, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28409868/validated-uhplc-ms-ms-assay-for-quantitative-determination-of-etoposide-gemcitabine-vinorelbine-and-their-metabolites-in-lung-cancer-patients
#20
Xiaobin Gong, Le Yang, Feng Zhang, Youtian Liang, Shouhong Gao, Ke Liu, Wansheng Chen
A fully valid ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC- MS/MS) method was developed for the determination of etoposide, gemcitabine, vinorelbine and their metabolites (etoposide catechol, 2',2'-difluorodeoxyuridine and 4-O-deacetylvinorelbine) in human plasma. Multiple reaction monitoring (MRM) mode was performed with an electrospray ionization (ESI) interface operating in both the positive and negative ion modes per compound. The method required only 100 μL plasma with a one-step simple de-proteinization procedure, and a short run time of 7...
April 13, 2017: Biomedical Chromatography: BMC
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