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https://www.readbyqxmd.com/read/28550720/development-and-validation-of-an-uplc-uv-method-for-determination-of-a-novel-triazine-coccidiostat-ethanamizuril-and-its-metabolite-m3-in-chicken-tissues
#1
Xiao Zhao, Youfa Xu, Lifang Zhang, Chunmei Wang, Chunna Guo, Chenzhong Fei, Keyu Zhang, Xiaoyang Wang, Yingchun Liu, Mi Wang, Wenli Zheng, Feiqun Xue
Ethanamizuril (EZL) is a novel anti-coccidial triazine synthesized by the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences. As a novel coccidiostat, it is essential to study its residue in food animal tissues. In this study, a reliable UPLC method for the determination of ethanamizuril (EZL) and one of its metabolites (M3) in chicken muscle, skin and fat, liver, and kidney samples was developed. Analytes were extracted using acetonitrile and 10% sodium carbonate solution, defatted by n-hexane, and further purified by using an Oasis MCX cartridge...
May 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28550653/phytochemical-profile-and-angiotensin-i-converting-enzyme-ace-inhibitory-activity-of-limonium-michelsonii-lincz
#2
Janar Jenis, Jeong Yoon Kim, Zia Uddin, Yeong Hun Song, Hyeong-Hwan Lee, Ki Hun Park
Members of the genus Limonium are widely used as medicinal herbs due to their health-promoting effects, such as an ability to improve blood circulation by inhibiting angiotensin I converting enzyme (ACE). While the potential of L. michelsonii Lincz. (a medicinal plant endemic to Kazakhstan) to inhibit ACE has been demonstrated, the inhibitory activities of its secondary metabolites have not been explored. In this work, the principal phenolic compounds (1-20) among these metabolites were isolated to determine the components responsible for ACE inhibition...
May 26, 2017: Journal of Natural Medicines
https://www.readbyqxmd.com/read/28550076/high-throughput-analysis-of-sulfatides-in-cerebrospinal-spinal-fluid-using-automated-extraction-and-uplc-ms-ms
#3
Mara Blomqvist, Jan Bor Eacuten, Henrik Zetterberg, Kaj Blennow, Jan-Erik M Aringnsson, Marcus St Aringhlman
Sulfatides are a group of glycosphingolipids that are highly expressed in brain. Due to their importance for normal brain function and their potential involvement in neurological diseases, development of accurate and sensitive methods for their determination are needed. Here we describe a high-throughput oriented and quantitative method for the determination of sulfatides in cerebrospinal fluid. The sulfatides are extracted using a fully automated liquid/liquid extraction method and quantified using ultra-performance liquid chromatography coupled to tandem mass spectrometry...
May 26, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28547736/rice-bran-metabolome-contains-amino-acids-vitamins-cofactors-and-phytochemicals-with-medicinal-and-nutritional-properties
#4
Iman Zarei, Dustin G Brown, Nora Jean Nealon, Elizabeth P Ryan
BACKGROUND: Rice bran is a functional food that has shown protection against major chronic diseases (e.g. obesity, diabetes, cardiovascular disease and cancer) in animals and humans, and these health effects have been associated with the presence of bioactive phytochemicals. Food metabolomics uses multiple chromatography and mass spectrometry platforms to detect and identify a diverse range of small molecules with high sensitivity and precision, and has not been completed for rice bran...
December 2017: Rice
https://www.readbyqxmd.com/read/28547389/anti-inflammation-compounds-from-the-seedpods-of-pongamia-pinnata-l-pierre-guided-by-the-bioactivity-and-uplc-hresims
#5
Hong Guo, Zisong Bai, Yangyang Xu, Xueqin Wu, Ning Li, Yan Zhu, Xiaoming Wang, Peng Zhang
Pongamia pinnata (Linn.) Pierre has anti-inflammatory activity and could significantly decrease serum tumor necrosis factor-α and IL-10 in arthritic rats. Previous research indicated the typical chemical constituent in P. pinnata is furanoflavone. Guided by anti-inflammatory active assay and UPLC-HRESIMS chromatography, 22 compounds were isolated from the ethanol extract of P. pinnata seedpods. One novel furanoflavone, 4'-hydroxypinnatin, was elucidated by HRESIMS, 1D- and 2D-NMR spectra. The 21 known compounds, including 9 furanoflavone, were identified by comparing their NMR data with the previous data in reference...
May 25, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28545666/mahuannin-b-an-adenylate-cyclase-inhibitor-attenuates-hyperhidrosis-via-suppressing-%C3%AE-2-adrenoceptor-camp-signaling-pathway
#6
Zengyong Wang, Yu Cui, Guoyu Ding, Mengge Zhou, Xiaoyao Ma, Yuanyuan Hou, Min Jiang, Dailin Liu, Gang Bai
BACKGROUND: Based on the traditional application of traditional Chinese Medicines (TCMs), Ephedra Herba (EH) is used to cure cold fever by inducing sweating, whereas Ephedra Radix (ER) is used to treat hyperhidrosis. Although they come from the same plant, Ephedra sinica Stapf, but have play opposing roles in clinical applications. EH is known to contain ephedrine alkaloids, which is the driver of the physiological changes in sweating, heart rate and blood pressure. However, the active pharmacological ingredients (APIs) of ER and the mechanisms by which it restricts sweating remain unknown...
July 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28544884/a-validated-uhplc-qtof-ms-method-for-quantification-of-metformin-and-teneligliptin-in-rat-plasma-application-to-pharmacokinetic-interaction-study
#7
David Paul, Lingesh Allakonda, Nanjappan Satheeshkumar
In this study a sensitive UHPLC-QTOF-MS method was developed and validated for the quantitation of the metformin (MET) and teneligliptin (TEN) in rat plasma using dapagliflozin as an internal standard (IS). Chromatographic separation were carried out on a Acquity UPLC HSS Cyano column (100mm x 2.1mm, 1.8μm) using gradient mobile phase system consisting of 0.1% formic acid and acetonitrile at a flow rate of 0.4mL/min, within a run time of 6min. Protein precipitation method was used as sample preparation approach...
May 15, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28544073/hydrophilic-interaction-and-reversed-phase-ultra-performance-liquid-chromatography-tof-ms-for-metabolomic-analysis-of-veratrum-nigrum-induced-cardiotoxicity
#8
Ziheng Wei, Xu Dong, Hanzhe Zhang, Songyan Gao, Wei Shi, Feng Yang, Xin Dong
The acute cardiotoxicity induced by Veratrum nigrum (VN) is explored by analysing heart tissue metabolic profiles in mouse models and applying reversed-phase liquid chromatography mass spectrometry (RPLC-MS) and hydrophilic interaction liquid chromatography mass spectrometry (HILIC-MS) that are based on ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). An animal model of acute heart injury was established in mice via intra-gastric administration of VN. Then, electrocardiogram, echocardiograph monitoring of cardiac function and pathological examination were performed on mice both in the control and VN groups, and it was successfully verified that acute heart injury was caused...
May 22, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28544042/forced-degradation-studies-of-lansoprazole-using-lc-esi-hrms-and-1-h-nmr-experiments-in-vitro-toxicity-evaluation-of-major-degradation-products
#9
G Shankar, R M Borkar, S Udutha, L Guntuku, V G M Naidu, N Nagesh, R Srinivas
Regulatory agencies from all over the world have set up stringent guidelines with regard to drug degradation products due to their toxic effects or carcinogenicity. Lansoprazole, a proton-pump inhibitor, was subjected to forced degradation studies as per ICH guidelines Q1A (R2). The drug was found to degrade under acidic, basic, neutral hydrolysis and oxidative stress conditions, whereas it was found to be stable under thermal and photolytic conditions. The chromatographic separation of the drug and its degradation products were achieved on a Hiber Purospher, C18 (250 X 4...
May 20, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28543613/comparative-pharmacokinetics-of-acteoside-from-total-glycoside-extracted-from-leaves-of-rehmannia-and-dihuangye-total-glycoside-capsule-in-normal-and-diabetic-nephropathy-rats
#10
Xin-Xin Dai, Shu-Lan Su, Hong-Die Cai, Dan-Dan Wei, Tian-Yao Zheng, Zhen-Hua Zhu, Hui Yan, Er-Xin Shang, Sheng Guo, Da-Wei Qian, Jin-Ao Duan
Rehmannia glutinosa Libosch (RG), is officially listed in the Chinese Pharmacopoeia and is widely used in China. In this paper, a sensitive and rapid ultra-performance liquid chromatography-mass spectrometry (UPLC-TQ-MS) including multiple-reaction monitoring mode was developed and applied to study the pharmacokinetics effect of acteoside from Total glycoside Extracted from Leaves of Rehmannia (TLR) and Dihuangye total glycoside capsule (DTG) in normal and diabetic nephropathy rats. Diabetic nephropathy rats model was induced by intraperitoneal injection of a small dose of streptozotocin and high-fat diet and plus 5% glucose drinking water...
May 23, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28543360/relationship-between-the-uplc-q-tof-ms-fingerprinted-constituents-from-daphne-genkwa-and-their-anti-inflammatory-anti-oxidant-activities
#11
Wen-Juan Du, Jun Ji, Ling Wang, Xin-Yi Lan, Jia Li, Jun-Qiu Lei, Xin He, Chun-Feng Zhang, Wen-Ze Huang, Zhen-Zhong Wang, Wei Xiao, Chong-Zhi Wang, Chun-Su Yuan
Daphne genkwa Sieb.et Zucc. is a well-known medicinal plant. This study was designed to apply the ultra-high performance liquid chromatography (UPLC) system to establish a quality control method of Daphne genkwa. Data revealed that there were fifteen common peaks in ten batches of D. genkwa Sieb. Et Zucc.(Thymelaeaceae) from different provinces of China. On this basis, the fingerprint chromatogram was established to provide references for quality control. Afterwards, the chemical constitutions of these common peaks were analyzed using the UPLC-Q-TOF-MS system and nine of them were identified primarily...
May 22, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28541461/screening-with-quantification-for-64-drugs-and-metabolites-in-human-urine-using-uplc-ms-ms-analysis-and-a-threshold-accurate-calibration
#12
Thomas G Rosano, Patrice Y Ohouo, Michelle Wood
Drug and metabolite (analytes) identification together with quantification is an important analytical tool in forensic and clinical toxicology. We report the development and validation of a definitive detection and quantification method (UPLC-MS-MS) for initial screening of 64 analytes in urine. The principle of the method is a quantitative extension of a recently reported threshold accurate calibration (TAC) technique which employs a rapid dual-specimen analysis i.e., with and without addition of a reference-analyte standard for normalization of matrix effects...
May 24, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/28541451/uplc-q-tof-ms-based-metabolic-profiling-comparison-of-two-major-bioactive-components-and-their-metabolites-in-normal-and-ckd-rat-plasma-urine-and-feces-following-oral-administration-of-fructus-corni-extract
#13
Min Zhao, Jinhua Tao, Leyue Du, Shu Jiang, Dawei Qian, Jinao Duan
Fructus Corni has been used for nourishing liver and kidney in clinical practice for many years. However, the in vivo integrated metabolism profile of its bioactive components remains unknown. In the present study, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry coupled with the MetaboLynxTM software was established for screening and identification of the metabolites of the loganin and the morroniside in normal and chronic kidney disease (CKD) rat plasma, urine and feces after oral administration of Fructus Corni extract...
May 24, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28540856/an-uplc-msms-method-to-measure-plasma-homocysteine-concentration
#14
Caroline Vayne, Luc Deroche, Nicolas Drillaud, Franck Patin, Clément Bruno, François Maillot, François Labarthe, Philippe Corcia, Isabelle Benz-de Bretagne, Christian R Andres, Patrick Emond, Hélène Blasco
Homocysteine (Hcy) is monitored in a growing number of diseases and requires a rapid and reliable method to measure its concentration in routine practice. We validated a new mass spectrometry method to measure plasma Hcy concentration and to determinate our own targeted concentrations according to COFRAC (French accreditation committee) recommendations. We collected the Hcy concentrations measured in the laboratory from 2014 to 2015 and we compared the values between different clinical groups. We obtained excellent performances of reproducibility, sensitivity (coefficient of variation <10%)...
June 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28540172/nonlinear-relationship-between-enteric-coated-mycophenolate-sodium-dose-and-mycophenolic-acid-exposure-in-han-kidney-transplantation-recipients
#15
Jun Zhang, Mengmeng Jia, Lihua Zuo, Na Li, Yonggang Luo, Zhi Sun, Xiaojian Zhang, Zhenfeng Zhu
The aim of the research was to investigate the pharmacokinetics (PK) of enteric-coated mycophenolate sodium (EC-MPS) by quantification of the active metabolite of mycophenolic acid (MPA) after multiple escalating oral doses in Han kidney transplant recipients. A total of 28 Han postoperative kidney transplant recipients were given a multiple-dose of 540, 720 or 900 mg of EC-MPS two times a day in combination with tacrolimus for 6 days. Blood specimens were collected at each time point from 0 to 12 h after EC-MPS administration...
May 2017: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/28539642/combinatory-evaluation-of-transcriptome-and-metabolome-profiles-of-low-temperature-induced-resistant-ascites-syndrome-in-broiler-chickens
#16
Shourong Shi, Yiru Shen, Shan Zhang, Zhenhua Zhao, Zhuocheng Hou, Huaijun Zhou, Jianmin Zou, Yuming Guo
To select metabolic biomarkers and differentially expressed genes (DEGs) associated with resistant-ascites syndrome (resistant-AS), we used innovative techniques such as metabolomics and transcriptomics to comparatively examine resistant-AS chickens and AS controls. Metabolomic evaluation of chicken serum using ultra-performance liquid chromatography-quadruple time-of-flight high-sensitivity mass spectrometry (UPLC-QTOF/HSMS) showed significantly altered lysoPC(18:1), PE(18:3/16:0), PC(20:1/18:3), DG(24:1/22:6/0:0), PS(18:2/18:0), PI(16:0/16:0), PS(18:0/18:1), PS(14:1/14:0), dihydroxyacetone, ursodeoxycholic acid, tryptophan, L-valine, cycloserine, hypoxanthine, and 4-O-Methylmelleolide concentrations on day 21 and LysoPC(18:0), LysoPE(20:1/0:0), LysoPC(16:0), LysoPE(16:0/0:0), hypoxanthine, dihydroxyacetone, 4-O-Methylmelleolide, LysoPC(18:2), and PC(14:1/22:1) concentrations on day 35, between the susceptible and resistant groups...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28538661/mass-spectrometry-based-profiling-and-imaging-of-various-ginsenosides-from-panax-ginseng-roots-at-different-ages
#17
Jae Won Lee, Seung-Heon Ji, Young-Seob Lee, Doo Jin Choi, Bo-Ram Choi, Geum-Soog Kim, Nam-In Baek, Dae Young Lee
(1) Background: Panax ginseng root is one of the most important herbal products, and the profiling of ginsenosides is critical for the quality control of ginseng roots at different ages in the herbal markets. Furthermore, interest in assessing the contents as well as the localization of biological compounds has been growing. The objective of this study is to carry out the mass spectrometry (MS)-based profiling and imaging of ginsenosides to assess ginseng roots at different ages; (2) Methods: Optimal ultra performance liquid chromatography coupled to quadrupole time of flight/MS (UPLC-QTOF/MS) was used to profile various ginsenosides from P...
May 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28538203/uhplc-ms-ms-method-with-sample-dilution-to-test-therapeutic-adherence-through-quantification-of-ten-antihypertensive-drugs-in-urine-samples
#18
Amedeo De Nicolò, Valeria Avataneo, Franco Rabbia, Mauro Sciandra, Francesco Tosello, Jessica Cusato, Elisa Perlo, Paolo Mulatero, Franco Veglio, Giovanni Di Perri, Antonio D'Avolio
Nowadays, hypertension represents an important health problem, particularly in developed countries. In some cases the standard therapeutic approaches are not able to reestablish the normal blood pressure values: this condition is called "resistant hypertension". However, a fraction of cases of resistant hypertension are actually due to poor adherence to the prescribed therapy. Therapeutic Drug Monitoring could represent a direct and useful tool to correctly identify non-compliant patients. Nevertheless, high throughput methods for the simultaneous monitoring of a wide panel of drugs in the same analysis are lacking and, furthermore, there is not a generally acknowledged "standard" matrix for this test (plasma or urine)...
May 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28535676/uplc-ms-it-tof-identification-of-circumdatins-produced-by-a-ochraceus
#19
Jesus Maria Gonzalez, Amparo Alfonso, M J Sainz, Mercedes R Vieytes, Luis M Botana
A method based on the combined use of ultra performance liquid chromatography coupled to mass spectrometry - ion trap - time of flight (UPLC-MS-IT-TOF) detection was employed to identify the metabolite production of Aspergillus ochraceus, which is the major cause of food and feed contamination due to ochratoxin A (OTA). Under the proposed chromatographic conditions, the separation and identification of seven metabolites belonging to the family of circumdatins was achieved. Their initial identification was performed through the exact molecular formula, as a function of their accurate mass...
May 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28535078/complementation-of-ctb7-in-the-maize-pathogen-cercospora-zeina-overcomes-the-lack-of-in-vitro-cercosporin-production
#20
Velushka Swart, Bridget Genevieve Crampton, John Ridenour, Burton Bluhm, Nicholas Olivier, Marion Meyer, Dave Kenneth Berger
Gray leaf spot (GLS), caused by the sibling species Cercospora zeina or Cercospora zeae-maydis, is cited as one of the most important diseases threatening global maize production. C. zeina fails to produce cercosporin in vitro, and in most cases causes large coalescing lesions during maize infection; a symptom generally absent from cercosporin-deficient mutants in other Cercospora spp. Here we describe the C. zeina cercosporin toxin biosynthetic gene cluster. The oxidoreductase gene CTB7 contained several insertions and deletions as compared to the C...
May 23, 2017: Molecular Plant-microbe Interactions: MPMI
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