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https://www.readbyqxmd.com/read/28644879/identification-of-a-novel-fungus-trichoderma-asperellum-gdfs1009-and-comprehensive-evaluation-of-its-biocontrol-efficacy
#1
Qiong Wu, Ruiyan Sun, Mi Ni, Jia Yu, Yaqian Li, Chuanjin Yu, Kai Dou, Jianhong Ren, Jie Chen
Due to its efficient broad-spectrum antimicrobial activity, Trichoderma has been established as an internationally recognized biocontrol fungus. In this study, we found and identified a novel strain of Trichoderma asperellum, named GDFS1009. The mycelium of T. asperellum GDFS1009 exhibits a high growth rate, high sporulation capacity, and strong inhibitory effects against pathogens that cause cucumber fusarium wilt and corn stalk rot. T. asperellum GDFS1009 secretes chitinase, glucanase, and protease, which can degrade the cell walls of fungi and contribute to mycoparasitism...
2017: PloS One
https://www.readbyqxmd.com/read/28644028/understanding-the-effects-of-genotype-growing-year-and-breeding-on-tunisian-durum-wheat-allergenicity-part-1-the-baker-s-asthma-case
#2
Fatma Boukid, Barbara Prandi, Stefano Sforza, Rhouma Sayar, Yong Weon Seo, Mondher Mejri, Ines Yacoubi
Baker's asthma is a serious airway disease triggered by wheat proteins CM3 α-amylase/trypsin inhibitor. The purpose of the present study was to investigate the impact of genotype and crop year on allergen CM3 α-amylase/trypsin inhibitor associated to baker's asthma. A historical series of Tunisian durum wheat (100 accessions), derived from three crop years, was used to compare the amount of CM3 from landraces to advanced cultivars. CM3 protein quantification was assessed after an enzymatic cleavage of the soluble protein extracts on an UPLC/ESI-MS system, using a marker peptide for its quantification...
June 23, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28643517/a-novel-strategy-for-comprehensive-profiling-and-identification-of-acidic-glycosphingolipids-using-ultra-high-performance-liquid-chromatography-coupled-with-quadrupole-time-of-flight-mass-spectrometry
#3
Ting Hu, Zhixin Jia, Jinlan Zhang
Acidic glycosphingolipids (AGSLs), which mainly consist of ganglioside and sulfatide moieties, are highly concentrated in the central nervous system. Comprehensive profiling of AGSLs has historically been challenging because of their high complexity and the lack of standards. In this paper, a novel strategy was developed to comprehensively profile AGSLs using ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS). Ganglioside isomers with different glycan chains such as GD1a/ GD1b were completely separated on a C18 column for the first time to our knowledge, facilitated by the addition of formic acid in the mobile phase...
June 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28643437/silibinin-affects-the-pharmacokinetics-of-methadone-in-rats
#4
Pei-Pei Pan, Jun Wang, Jun Luo, Shuang-Hu Wang, Yun-Fang Zhou, Sai-Zhen Chen, Zhou Du
The aim of the present study was to investigate the pharmacokinetic effect of silibinin on methadone in rats. 24 male Sprague-Dawley rats were randomly divided into 4 groups: control group, single dose of 100 mg/kg group, multiple doses of 100 mg/kg group and multiple doses of 30 mg/kg group. A single dose of 6 mg/kg methadone was administrated to rats orally without or with silibinin. Plasma samples were collected via tail vein at different time points and concentrations of methadone and its metabolite, EDDP, were determined by UPLC-MS/MS...
June 22, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28641225/investigation-of-dissociation-constants-for-individual-and-total-naphthenic-acids-species-using-ultra-performance-liquid-chromatography-ion-mobility-time-of-flight-mass-spectrometry-analysis
#5
Rongfu Huang, Pamela Chelme-Ayala, Yanyan Zhang, Mohamed Changalov, Mohamed Gamal El-Din
Ultra-performance liquid chromatography ion mobility time-of-flight mass spectrometry (UPLC-IM-TOFMS) was utilized for the analysis of naphthenic acids (NAs) in fractions of pH-dependent sequential liquid-liquid extractions from oil sands process-affected water. Ion-mobility separation technique allowed the differentiation of OyS-NAs (2 ≤ y ≤ 4) from Ox-NAs (2 ≤ x ≤ 5) via drift time versus retention time separations. The results indicated that the addition of S atom to the O2-NA molecule led to a lower increase in the dissociation constant (pKa) compared to that caused by the addition of O atom...
June 18, 2017: Chemosphere
https://www.readbyqxmd.com/read/28636885/differential-changes-in-the-pharmacokinetics-of-statins-in-collagen-induced-arthritis-rats
#6
Chun-Han Lin, Ke-Wei Hsu, Chia-Hao Chen, Yow-Shieng Uang, Chun-Jung Lin
The elevated systemic levels of cytokines in rheumatoid arthritis (RA) can change the expression of metabolic enzymes and transporters. Given that statins are lipid-lowering agents frequently used in RA patients with concurrent cardiovascular diseases, the objective of the present study was to investigate the impacts of RA on the pharmacokinetics of statins of different disposition properties in rats with collagen-induced arthritis (CIA). The expression of metabolic enzymes and transporters in tissues of CIA rats were analyzed by RT-qPCR...
June 18, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28636672/metabolomic-profile-and-nucleoside-composition-of-cordyceps-nidus-sp-nov-cordycipitaceae-a-new-source-of-active-compounds
#7
Juan Chiriví, Giovanna Danies, Rocio Sierra, Nicolas Schauer, Sandra Trenkamp, Silvia Restrepo, Tatiana Sanjuan
Cordyceps sensu lato is a genus of arthropod-pathogenic fungi, which have been used traditionally as medicinal in Asia. Within the genus, Ophiocordyceps sinensis is the most coveted and expensive species in China. Nevertheless, harvesting wild specimens has become a challenge given that natural populations of the fungus are decreasing and because large-scale culture of it has not yet been achieved. The worldwide demand for products derived from cultivable fungal species with medicinal properties has increased recently...
2017: PloS One
https://www.readbyqxmd.com/read/28635280/anti-adhesive-activity-and-metabolomics-analysis-of-rat-urine-after-cranberry-vaccinium-macrocarpon-aiton-administration
#8
Gregorio Peron, Pellizzaro Anna, Brun Paola, Elisabetta Schievano, Stefano Mammi, Stefania Sut, Ignazio Castagliuolo, Stefano Dall'Acqua
Cranberry (Vaccinium macrocarpon Aiton) is used to treat non-complicated urinary tract infections (UTIs). A-type procyanidins (PAC-A) are considered the active constituents being able to inhibit bacterial adhesion to the urinary epithelium. However, there are doubts about PAC-A role in UTIs, due to their poor bioavailability, extensive metabolism, limited knowledge about urinary excretion and contradictory clinical trials. The effects of 35 days cranberry (11 mg/kg PAC-A, 4 mg/kg PAC-B) supplementation were studied in healthy rats using a UPLC-MS-based metabolomics approach...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28635270/understanding-the-effects-of-genotype-growing-year-and-breeding-on-tunisian-durum-wheat-allergenicity-part-2-the-celiac-disease-case
#9
Fatma Boukid, Barbara Prandi, Stefano Sforza, Rhouma Sayar, Yong Weon Seo, Mondher Mejri, Ines Yacoubi
The aim of this study was to compare immunogenic and toxic gluten peptides related to celiac disease (CD). 100 accessions of genotypes selected during the 20th century in Tunisia were in vitro digested, then analyzed by UPLC/ESI-MS technique using an isotopically labeled internal standard. The first MANOVA confirmed a high variability in the content of immunogenic and toxic peptides reflecting high genetic diversity in the germplasm released during the last century in Tunisia, consistently with PCA and clustering analysis results...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28632497/dha-reduces-oxidative-stress-following-hypoxia-ischemia-in-newborn-piglets-a-study-of-lipid-peroxidation-products-in-urine-and-plasma
#10
Marianne Ullestad Huun, Håvard T Garberg, Javier Escobar, Consuelo Chafer, Maximo Vento, Ingar M Holme, Ola Didrik Saugstad, Rønnaug Solberg
BACKGROUND: Lipid peroxidation mediated by reactive oxygen species is a major contributor to oxidative stress. Docosahexaenoic acid (DHA) has anti-oxidant and neuroprotective properties. Our objective was to assess how oxidative stress measured by lipid peroxidation was modified by DHA in a newborn piglet model of hypoxia-ischemia (HI). METHODS: Fifty-five piglets were randomized to (i) hypoxia, (ii) DHA, (iii) hypothermia, (iv) hypothermia+DHA or (v) sham. All groups but sham were subjected to hypoxia by breathing 8% O2...
June 20, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28631226/introduction-of-a-simple-second-tier-screening-test-for-c5-isobars-in-dried-blood-spots-reducing-the-false-positive-rate-for-isovaleric-acidaemia-in-expanded-newborn-screening
#11
R S Carling, D Burden, I Hutton, R Randle, K John, J R Bonham
In 2015 the English Newborn Screening programme expanded to include Isovaleric Acidaemia (IVA). Screening is performed by flow injection analysis tandem mass spectrometry of isovalerylcarnitine. Isovalerylcarnitine is isobaric with pivaloylcarnitine which can be present in blood due to the use of pivalic ester pro-drugs or pivalic acid derivatives used as emollients in some nipple creams; the potential for false positives (FP) is well documented. A pilot study in England screened 438,164 babies, 18 had presumptive positive results but only 4 were confirmed as true positives (TP)...
June 20, 2017: JIMD Reports
https://www.readbyqxmd.com/read/28629539/uplc-esi-qtof-ms-based-metabolomics-survey-on-the-toxicity-of-triptolide-and-detoxication-of-licorice
#12
Zhuo Wang, Jian-Qun Liu, Jin-Di Xu, He Zhu, Ming Kong, Guo-Hua Zhang, Su-Min Duan, Xiu-Yang Li, Guang-Fu Li, Li-Fang Liu, Song-Lin Li
Triptolide (TP) from Tripterygium wilfordii has been demonstrated to possess anti-inflammatory, immunosuppressive, and anticancer activities. TP is specially used for the treatment of awkward rheumatoid arthritis, but its clinical application is confined by intense side effects. It is reported that licorice can obviously reduce the toxicity of TP, but the detailed mechanisms involved have not been comprehensively investigated. The current study aimed to explore metabolomics characteristics of the toxic reaction induced by TP and the intervention effect of licorice water extraction (LWE) against such toxicity...
June 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28628838/validated-uplc-ms-ms-method-for-simultaneous-quantification-of-eight-saikosaponins-in-rat-plasma-application-to-a-comparative-pharmacokinetic-study-in-depression-rats-after-oral-administration-of-extracts-of-raw-and-vinegar-baked-bupleuri-radix
#13
Tianli Lei, Dandan Zhang, Kun Guo, Mingxiao Li, Chongning Lv, Jing Wang, Lingyun Jia, Jincai Lu
A sensitive and efficient UPLC-MS/MS method was developed and validated for simultaneous quantification of eight saikosaponins (SSa, SSb1, SSb2, SSb3, SSb4, SSc, SSd and SSf) in rat plasma. Furthermore, comparative pharmacokinetic profiles of these sakosaponins were investigated, following oral administration of extracts of raw and vinegar-baked Bupleuri Radix to depression rats. Biosamples were processed with liquid-liquid extraction technique using ethyl acetate. Chromatographic separation was accomplished on Waters BEH C18 UPLC column...
June 16, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28628837/the-influence-of-essential-oils-from-xiang-fu-si-wu-decoction-on-its-non-volatile-components-and-its-application-for-pharmacokinetics-in-normal-rats
#14
Ying Pan, Dawei Qian, Pei Liu, Yang Zhang, Zhenhua Zhu, Li Zhang, Jin-Ao Duan
Xiang-Fu-Si-Wu Decoction (XFSWD) has been used to treat gynecology diseases in clinical practice for hundreds of years in China, especially for primary dysmenorrheal of QiZhi Type. In previous researches, both essential oils (XEO) and non-volatile components (NXEO) extracted from XFSWD showed significant pharmacological activities, but the influence of XEO on NXEO was unclear. The purpose of this paper was to develop a validated analytical method for simultaneous determination of ten components including Vanillic acid, Ferulic acid, Caffeic acid, Gallic acid, Paeoniflorin, Albiflorin, Tetrahydropalmatine, Protopine, Berberine and Tetrahydrocolumbamine, and to compare the pharmacokinetic parameters of these components to illustrate the influences of XEO on NXEO in normal rats...
June 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28628333/coupling-targeted-and-untargeted-mass-spectrometry-for-metabolome-microbiome-wide-association-studies-of-human-fecal-samples
#15
Alexey V Melnik, Pieter C Dorrestein, Ricardo R da Silva, Embriette R Hyde, Alexander A Aksenov, Fernando Vargas, Amina Bouslimani, Ivan Protsyuk, Alan Jarmusch, Anupriya Tripathi, Theodore Alexandrov, Rob Knight
Increasing appreciation of the gut microbiome's role in health motivates understanding the molecular composition of human feces. To analyze such complex samples, we developed a platform coupling targeted and untargeted metabolomics. The approach is facilitated through split flow from one UPLC, joint timing triggered by contact closure relays and a script to retrieve the data. It is designed to detect specific metabolites of interest with high sensitivity, allows for correction of targeted information, enables better quantitation thus providing an advanced analytical tool for exploratory studies...
June 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28626037/autopolyploidization-geographic-origin-and-metabolome-evolution-in-arabidopsis-thaliana
#16
Fredd Vergara, Bart Rymen, Ayuko Kuwahara, Yuji Sawada, Muneo Sato, Masami Y Hirai
PREMISE OF THE STUDY: Autopolyploidy, or whole-genome duplication, is a recurrent phenomenon in plant evolution. Its existence can be inferred from the presence of massive levels of genetic redundancy revealed by comparative plant phylogenomics. Whole-genome duplication is theoretically associated with evolutionary novelties such as the development of new metabolic reactions and therefore contributes to the evolution of new plant metabolic profiles. However, very little is yet known about the impact of autopolyploidy on the metabolism of recently formed autopolyploids...
June 16, 2017: American Journal of Botany
https://www.readbyqxmd.com/read/28623927/caffeoylquinic-acid-derivatives-rich-extract-from-gnaphalium-pensylvanicum-willd-ameliorates-hyperuricemia-and-acute-gouty-arthritis-in-animal-model
#17
Yan Jiang, Yan Lin, Yi-Juan Hu, Xiao-Jun Song, Hong-Hua Pan, Hong-Jian Zhang
BACKGROUND: The Gnaphalium pensylvanicum willd. is used in China as a folk medicine to treat anti-inflammatory, cough and rheumatism arthritis. The aim of this study was to evaluate the potential of the extract of G. pensylvanicum to treat hyperuricemia and acute gouty arthritis in animal model. METHODS: G. pensylvanicum extract was evaluated in an experimental model with potassium oxonate (PO) induced hyperuricemia in mice which was used to evaluate anti-hyperuricemia activity and xanthine oxidase (XO) inhibition...
June 17, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28622994/ultrasound-assisted-acid-hydrolysis-of-cellulose-to-chemical-building-blocks-application-to-furfural-synthesis
#18
Daniel Santos, Ubiratan F Silva, Fabio A Duarte, Cezar A Bizzi, Erico M M Flores, Paola A Mello
In this work, the use of ultrasound energy for the production of furanic platforms from cellulose was investigated and the synthesis of furfural was demonstrated. Several systems were evaluated, as ultrasound bath, cup horn and probe, in order to investigate microcrystalline cellulose conversion using simply a diluted acid solution and ultrasound. Several acid mixtures were evaluated for hydrolysis, as diluted solutions of HNO3, H2SO4, HCl and H2C2O4. The influence of the following parameters in the ultrasound-assisted acid hydrolysis (UAAH) were studied: sonication temperature (30 to 70°C) and ultrasound amplitude (30 to 70% for a cup horn system) for 4 to 8molL(-1) HNO3 solutions...
April 25, 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28618388/simultaneous-determination-of-nine-%C3%AE-lactam-antibiotics-in-human-plasma-by-an-ultrafast-hydrophilic-interaction-chromatography-tandem-mass-spectrometry
#19
Alan Abdulla, Soma Bahmany, Rixt A Wijma, Bart C H van der Nagel, Birgit C P Koch
Contemporary β-lactam antibiotic dosing is debatable in severely ill patients, since the occurrence of pathophysiological changes in critical illness can result in great inter-individual variability. Therapeutic drug monitoring (TDM) is a commonly used dosing strategy to optimize exposure and thereby minimize toxicity and maximize the efficacy. Currently, TDM of β-lactam antibiotics is rarely performed, due to poor availability in clinical practice. We describe an ultrafast Hydrophilic-Interaction Chromatography (HILIC) based UPLC-MS/MS method for the determination of amoxicillin, benzylpenicillin, cefotaxime, cefuroxime, ceftazidime, flucloxacillin, imipenem, meropenem and piperacillin in human plasma...
June 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28618281/chlor-am-ination-of-iopamidol-kinetics-pathways-and-disinfection-by-products-formation
#20
Fu-Xiang Tian, Bin Xu, Yi-Li Lin, Chen-Yan Hu, Tian-Yang Zhang, Sheng-Ji Xia, Wen-Hai Chu, Nai-Yun Gao
The degradation kinetics, pathways and disinfection by-products (DBPs) formation of iopamidol by chlorine and chloramines were investigated in this paper. The chlorination kinetics can be well described by a second-order model. The apparent second-order rate constants of iopamidol chlorination significantly increased with solution pH. The rate constants of iopamidol with HOCl and OCl(-) were calculated as (1.66 ± 0.09) × 10(-3) M(-1) s(-1) and (0.45± 0.02) M(-1) s(-1), respectively. However, the chloramination of iopamidol fitted well with third-order kinetics and the maximum of the apparent rate constant occurred at pH 7...
June 5, 2017: Chemosphere
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