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https://www.readbyqxmd.com/read/29474275/development-and-validation-of-a-simultaneous-quantification-method-of-ruxolitinib-vismodegib-olaparib-and-pazopanib-in-human-plasma-using-lc-ms-ms
#1
Claire Pressiat, Huu-Hien Huynh, Alain Plé, Hélène Sauvageon, Isabelle Madelaine, Cécile Chougnet, Christine Le Maignan, Samia Mourah, Lauriane Goldwirt
BACKGROUND: A simple, rapid, and sensitive LC-MS/MS method has been developed and validated for the quantification of ruxolitinib, olaparib, vismodegib, and pazopanib in human plasma. METHODS: After a simple protein precipitation of plasma samples, the chromatographic separation was performed using an UPLC system coupled with MS/MS in a positive ionization mode. The mobile phase consisted of a gradient elution of 10 mmol/L formate ammonium buffer containing 0.1% (v/v) formic acid (phase A) and acetonitrile with 0...
February 22, 2018: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/29473927/screening-and-identification-of-inhibitors-of-advanced-glycation-endproduct-formation-from-microalgal-extracts
#2
Peipei Sun, Ka-Wing Cheng, Yongjin He, Bin Liu, Xuemei Mao, Feng Chen
The formation and accumulation of advanced glycation endproducts (AGEs) have been implicated in the pathogenesis of many chronic diseases, such as aging, Alzheimer' s disease, diabetes and diabetic complications. The present study was aimed to investigate the inhibitory effects of the extracts from nine microalgae on the formation of AGEs by using in vitro models and identify key antiglycation constituents of the microalgae. A BSA-glucose model with simulated physiological conditions was used to evaluate the inhibitory effect on total AGE formation...
February 23, 2018: Food & Function
https://www.readbyqxmd.com/read/29473886/antioxidation-and-cytoprotection-of-acteoside-and-its-derivatives-comparison-and-mechanistic-chemistry
#3
Xican Li, Yulu Xie, Ke Li, Aizhi Wu, Hong Xie, Qian Guo, Penghui Xue, Yerkingul Maleshibek, Wei Zhao, Jiasong Guo, Dongfeng Chen
The study tried to explore the role of sugar-residues and mechanisms of phenolic phenylpropanoid antioxidants. Acteoside, along with its apioside forsythoside B and rhamnoside poliumoside, were comparatively investigated using various antioxidant assays. In three electron-transfer (ET)-based assays (FRAP, CUPRAC, PTIO•-scavenging at pH 4.5), the relative antioxidant levels roughly ruled as: acteoside >forsythoside B > poliumoside. Such order was also observed in H⁺-transfer-involved PTIO•-scavenging assay at pH 7...
February 23, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29472858/an-integrated-lipidomics-and-phenotype-study-reveals-protective-effect-and-biochemical-mechanism-of-traditionally-used-alisma-orientale-juzepzuk-in-chronic-kidney-disease
#4
Fang Dou, Hua Miao, Jing-Wen Wang, Lin Chen, Ming Wang, Hua Chen, Ai-Dong Wen, Ying-Yong Zhao
Alisma orientale Juzepzuk (AO) is widely used for various diuretic and nephropathic treatments in traditional Chinese medicines (TCM). In a clinical setting, AO is used as both a lipid-lowering and tubular interstitial fibrosis agent. However, the mechanisms of AO for the treatment of renal interstitial fibrosis and abnormal lipid metabolism are not well-understood. In this study, pharmacological and UPLC-HDMS-based lipidomic approaches were employed to investigate the lipid-lowering and tubular interstitial fibrosis effect of AO on rats with adenine-induced chronic kidney disease (CKD)...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29471254/uplc-ms-ms-assay-validation-for-tacrolimus-quantitative-determination-in-peripheral-blood-t-cd4-and-b-cd19-lymphocytes
#5
Paschoalina Romano, Maria da Luz Fernandes, Pérsio de Almeida Rezende Ebner, Nayara Duarte de Oliveira, Larissa Mitsue Okuda, Fabiana Agena, Maria Elizabete Mendes, Nairo Massakazu Sumita, Verônica Coelho, Elias David-Neto, Nelson Zocoler Galante
Monitoring tacrolimus (Tac) exposure in cell matrices enriched with lymphocytes can improve Tac therapeutic drug monitoring (TDM) in solid organ transplant recipients. An UPLC-MS/MS based assay for Tac quantification in peripheral blood T CD4+ and B CD19+ lymphocytes was developed. Peripheral blood mononuclear cells (PBMC) were obtained by density gradient centrifugation and highly purified (purity >90%) T CD4+ and B CD19+ cell suspensions were acquired by magnetic negative selection from whole blood of 6 healthy volunteers...
January 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29470453/uhplc-uv-method-for-simultaneous-determination-of-perindopril-arginine-and-indapamide-hemihydrate-in-combined-dosage-form-a-stability-indicating-assay-method
#6
Naser F Al-Tannak
Perindopril arginine and Indapamide hemihydrate in combination were proven to have a synergistic antihypertensive impact when compared with the use of each component alone. Therefore, a new Ultra-High Performance Liquid Chromatography coupled with Ultraviolet detector (UHPLC-UV) method has been developed and subsequently validated for simultaneous determination of the anti-hypertensive combination of Perindopril arginine and Indapamide hemihydrate. The separation of Perindopril arginine and Indapamide hemihydrate was achieved using a BEH C18 (1...
February 22, 2018: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/29468821/urinary-metabolomics-profiles-associated-to-bovine-meat-ingestion-in-humans
#7
Nadezda V Khodorova, Douglas N Rutledge, Marion Oberli, David Mathiron, Paulo Marcelo, Robert Benamouzig, Daniel Tomé, Claire Gaudichon, Serge Pilard
SCOPE: The impact of meat consumption on human health is widely examined in nutritional epidemiological studies, especially due to the connexion between the consumption of red and processed meat and the risk of colon cancer. Food questionnaires do not assess the exposure to different methods of meat cooking. This study aimed to identify biomarkers of the acute ingestion of bovine meat cooked with 2 different processes. METHODS AND RESULTS: Non-targeted UPLC-MS metabolite profiling was done on urine samples obtained from 24 healthy volunteers before and 8h after the ingestion of a single meal composed of intrinsically15 N labelled bovine meat, either cooked at 55 °C for 5 min or at 90 °C for 30 min...
February 22, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29468750/wheezing-preschool-children-with-early-onset-asthma-reveal-a-specific-metabolomic-profile
#8
Silvia Carraro, Sara Bozzetto, Giuseppe Giordano, Dania El Mazloum, Matteo Stocchero, Paola Pirillo, Stefania Zanconato, Eugenio Baraldi
BACKGROUND: Many children of preschool age present with recurrent wheezing. Most of them outgrow their symptoms, while some have early-onset asthma. Aim of this prospective preliminary study was to apply a metabolomic approach to see whether biochemical-metabolic urinary profiles can have a role in the early identification of the children with asthma. METHODS: Preschool children with recurrent wheezing were recruited and followed up for 3 years, after which they were classified as cases of transient wheezing or early-onset asthma...
February 22, 2018: Pediatric Allergy and Immunology
https://www.readbyqxmd.com/read/29465116/polyphenols-rich-extract-from-pleurotus-eryngii-with-growth-inhibitory-of-hct116-colon-cancer-cells-and-anti-inflammatory-function-in-raw264-7-cells
#9
Qiuhui Hu, Biao Yuan, Hang Xiao, Liyan Zhao, Xian Wu, Kanyasiri Rakariyatham, Lei Zhong, Yanhui Han, Benard Muinde Kimatu, Wenjian Yang
Edible mushrooms are rich sources of bioactive components. In this study, a polyphenol-rich extract, designated as PPEP, was isolated from an edible mushroom, Pleurotus eryngii. Using ultra high performance liquid chromatograph combined with triple time-of-flight mass spectrometry (UPLC-TOF/MS/MS), gallic acid monohydrate, 3-(3,4-dihydroxyphenyl)-propionic acid, methyl gallate, syringic acid, ellagic acid and catechin were identified in PPEP. This phenolic-rich extract PPEP exhibited anti-inflammatory effect in lipopolysaccharide-stimulated RAW264...
February 21, 2018: Food & Function
https://www.readbyqxmd.com/read/29463053/nutritional-composition-and-antioxidant-properties-of-the-fruits-of-a-chinese-wild-passiflora-foetida
#10
Ya Song, Xiao-Qun Wei, Mei-Ying Li, Xue-Wu Duan, Yuan-Ming Sun, Rui-Li Yang, Xiang-Dong Su, Ri-Ming Huang, Hong Wang
The aim of this work was to evaluate the main nutrients and their antioxidant properties of a Chinese wild edible fruit, Passiflora foetida , collected from the ecoregion of Hainan province, China. The analytical results revealed that P. foetida fruits were rich in amino acids (1097 mg/100 g in total), minerals (595.75 mg/100 g in total), and unsaturated fatty acids (74.18 g/100 g in total fat). The lyophilized powder of edible portion contained the higher polyphenols content than the inedible portion powder...
February 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29462910/dynamic-changes-in-phenolics-and-antioxidant-capacity-during-pecan-carya-illinoinensis-kernel-ripening-and-its-phenolics-profiles
#11
Xiaodong Jia, Huiting Luo, Mengyang Xu, Min Zhai, Zhongren Guo, Yushan Qiao, Liangju Wang
Pecan ( Carya illinoinensis ) kernels have a high phenolics content and a high antioxidant capacity compared to other nuts-traits that have attracted great interest of late. Changes in the total phenolic content (TPC), condensed tannins (CT), total flavonoid content (TFC), five individual phenolics, and antioxidant capacity of five pecan cultivars were investigated during the process of kernel ripening. Ultra-performance liquid chromatography coupled with quadruple time-of-flight mass (UPLC-Q/TOF-MS) was also used to analyze the phenolics profiles in mixed pecan kernels...
February 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29462800/anti-versus-pro-inflammatory-metabololipidome-upon-cupping-treatment
#12
Qi Zhang, Xiang Wang, Guifang Yan, Juan Lei, Yu Zhou, Lei Wu, Ting Wang, Xiao Zhang, Duyun Ye, Yongsheng Li
BACKGROUND/AIMS: This study aimed to explore the metabololipidome in mice upon cupping treatment. METHODS: A nude mouse model mimicking the cupping treatment in humans was established by administrating four cupping sets on the back skin for 15 minutes. UPLC-MS/ MS was performed to determine the PUFA metabolome in mice skin and blood before and after cupping treatment. The significantly changed lipids were administered in macrophages to assess the production of pro-inflammatory cytokines IL-6 and TNF-α by ELISA...
February 15, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29461009/metabolomic-responses-to-lumacaftor-ivacaftor-in-cystic-fibrosis
#13
Benjamin T Kopp, Scott McCulloch, Chandra L Shrestha, Shuzhong Zhang, Lisa Sarzynski, Frederick W Woodley, Don Hayes
BACKGROUND: Cystic fibrosis (CF) is a life-limiting disease caused by a defect in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Lumacaftor/Ivacaftor is a novel CFTR modulator approved for patients that are homozygous for Phe508del CFTR, but its clinical effectiveness varies amongst patients, making it difficult to determine clinical responders. Therefore, identifying biochemical biomarkers associated with drug response are clinically important for follow-up studies...
February 20, 2018: Pediatric Pulmonology
https://www.readbyqxmd.com/read/29459349/spatial-distribution-of-bisphenol-s-in-surface-water-and-human-serum-from-yangtze-river-watershed-china-implications-for-exposure-through-drinking-water
#14
Yanjian Wan, Wei Xia, Shunyi Yang, Xinyun Pan, Zhenyu He, Kurunthachalam Kannan
Bisphenol S (BPS) is an emerging environmental contaminant. The occurrence of this compound in humans and the environment is not well described. In this study, 120 surface water samples and 240 human serum samples were collected along the Yangtze River in 2015 for the determination of the occurrence of BPS. Surface water and human serum samples were extracted by solid phase extraction and liquid-liquid extraction, respectively, and analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)...
February 9, 2018: Chemosphere
https://www.readbyqxmd.com/read/29457805/urinary-metabolomic-study-of-the-antagonistic-effect-of-p-ginseng-in-rats-with-estrogen-decline-using-ultra-performance-liquid-chromatography-coupled-with-quadrupole-time-of-flight-mass-spectrometry
#15
He Lin, Zhongying Liu, Zifeng Pi, Lihui Men, Weijia Chen, Zhiqiang Liu
Estrogens are biologically active steroid hormones mainly released from the ovary by ovarian secretion of estrogen into the circulating blood to regulate or function at the distal target. Estrogens play an important role in the central nervous system, cardiovascular system and immune system, especially for post-menopausal women. Panax ginseng Mayer has been reported to relieve women's menopausal symptoms and affect estrogen activities. However, the mechanism of its estrogen regulation has not yet been clearly investigated...
February 19, 2018: Food & Function
https://www.readbyqxmd.com/read/29455091/an-uplc-ms-ms-method-to-determine-ct-707-and-its-two-metabolites-in-plasma-of-alk-positive-advanced-non-small-cell-lung-cancer-patients
#16
Cheng Cui, Pei Hu, Ji Jiang, Fansheng Kong, Hong Luo, Qian Zhao
CT-707, a mutant-selective inhibitor of an important cancer target, anaplastic lymphoma kinase (ALK), is designed to be a targeted therapeutic agent for non-small cell lung cancer (NSCLC) patients harboring ALK active and crizotinib resistant mutations. A rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the determination of CT-707 and its two metabolites (M1 and M2) in human plasma. The samples were purified by solid phase extraction (SPE) and separated on a BEH C18 column (2...
January 31, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29454961/first-line-anti-tuberculosis-drugs-induce-hepatotoxicity-a-novel-mechanism-based-on-a-urinary-metabolomics-platform
#17
Jun Cao, Yijun Mi, Cuilin Shi, Yicong Bian, Chenrong Huang, Zhijian Ye, Linsheng Liu, Liyan Miao
Tuberculosis (TB) has become a global public health and social threat. As clinical first-line drugs, rifampicin and isoniazid used in combination with pyrazinamide and ethambutol (the HRZE regimen) usually induce hepatotoxicity. However, the mechanisms underlying this phenomenon remain unclear, and studying the metabolic impact of co-treating TB patients with the HRZE regimen can provide new hepatotoxicity evidence. In this study, urine metabolites from TB patients were profiled using a high-resolution ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) platform...
February 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29454926/chinese-patent-medicine-xin-ke-shu-inhibits-ca-2-overload-and-dysfunction-of-fatty-acid-%C3%AE-oxidation-in-rats-with-myocardial-infarction-induced-by-lad-ligation
#18
Yong Yang, Hongmei Jia, Meng Yu, Chao Zhou, Lili Sun, Yang Zhao, Hongwu Zhang, Zhongmei Zou
Myocardial infarction (MI) occurs during a sustained insufficient blood supply to the heart, eventually leading to myocardial necrosis. Xin-Ke-Shu tablet (XKS) is a prescription herbal compound and a patented medicine extensively used in the clinical treatment of coronary heart disease (CHD). To understand the molecular mechanism of the XKS action against MI in detail, it is necessary to investigate the altered metabolome and related pathways coincident with clinical features. In this study, tissue-targeted metabonomics based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) were developed to explore the metabolic changes associated with XKS treatment in the heart tissue of rats with MI induced by a left anterior descending coronary artery ligation (LAD)...
February 3, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29454264/development-and-validation-of-uplc-ms-ms-assay-for-quantification-of-cladrin-absolute-bioavailability-and-dose-proportionality-study-in-rats
#19
Mamunur Rashid, Sandeep K Singh, Mohd Yaseen Malik, Sadaf Jahan, Swati Chaturvedi, Isha Taneja, Kanumuri Sivarama Raju, Zaiba Naseem, J R Gayen, Muhammad Wahajuddin
Cladrin, an isoflavone is a major bioactive constituent found in stem bark of Butea monosperma with remarkable osteogenic activity. A speedy and sensitive UPLC coupled tandem mass spectrometry (UPLC-MS/MS) method was developed, validated and successfully applied to bioavailability, blood partitioning, plasma protein binding, intravenous and multiple-dose oral pharmacokinetics of cladrin in rats. Separation was done on C18 column (5.0 μm, 4.6 × 50 mm) using mobile phase containing acetonitrile and 0.10% formic acid in the ratio of 65:35 (v/v) with 0...
January 31, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29453042/evaluation-of-the-anti-hypertensive-effect-of-tengfu-jiangya-tablet-by-combination-of-uplc-q-exactive-ms-based-metabolomics-and-itraq-based-proteomics-technology
#20
Yanpeng Tian, Feng Jiang, Yunlun Li, Haiqiang Jiang, Yanjun Chu, Lijuan Zhu, Weixing Guo
OBJECTIVE: Tengfu Jiangya tablet (TJT) is a traditional Chinese medicine formulation composed of Uncaria rhynchophylla and Semen raphani. It is a hospital preparation that is widely used in clinics for treating hypertension. A previous metabolomics study reported that TJT exerted a protective effect on hypertension by restoring impaired NO production, ameliorating the inflammatory state, and vascular remodeling. A clinical proteomics study also revealed five key target proteins during TJT intervention...
February 13, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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