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https://www.readbyqxmd.com/read/28086873/cecal-micrornaome-response-to-salmonella-enterica-serovar-enteritidis-infection-in-white-leghorn-layer
#1
Guixian Wu, Yukai Qi, Xiaoyi Liu, Ning Yang, Guiyun Xu, Liying Liu, Xianyao Li
BACKGROUND: Salmonella enterica serovar Enteritidis (SE) is a food-borne pathogen and of great threat to human health through consuming the contaminated poultry products. MicroRNAs (miRNAs) play an important role in different biological activities and have been shown to regulate the innate immunity in the bacterial infection. The objective of this study is to identify miRNAs associated with SE infection in laying chicken cecum. RESULTS: Average number of reads of three libraries constructed from infected and non-infected chickens was 12,476,156 and 10,866,976, respectively...
January 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28017550/a-novel-bioconversion-for-value-added-products-from-food-waste-using-musca-domestica
#2
Yi Niu, Dong Zheng, Binghua Yao, Zizhe Cai, Zhimin Zhao, Shengqing Wu, Peiqing Cong, Depo Yang
Food waste, as a major part of the municipal solid waste has been generated increasingly worldwide. Efficient and feasible utilization of this waste material for productivity process is significant for both economical and environmental reasons. In the present study, Musca domestica larva was used as the carrier to conduct a bioconversion with food waste to get the value-added maggot protein, oil and organic fertilizers. Methods of adult flies rearing, culture medium adjuvant selection, maggot culture conditions, stocking density and the valorization of the waste have been explored...
December 22, 2016: Waste Management
https://www.readbyqxmd.com/read/28012319/a-novel-fluorimetric-sensing-platform-for-highly-sensitive-detection-of-organophosphorus-pesticides-by-using-egg-white-encapsulated-gold-nanoclusters
#3
Xu Yan, Hongxia Li, Tianyu Hu, Xingguang Su
Assays for organophosphorus pesticides (OPs) with high sensitivity as well as on-site screening have been urgently required to protect ecosystem and prevent disease. Herein, a novel fluorimetric sensing platform was constructed for quantitative detection of OPs via tyrosinase (TYR) enzyme-controlled quenching of gold nanoclusters (AuNCs). One-step green synthetic approach was developed for the synthesis of AuNCs by using chicken egg white (CEW) as template and stabilizer. Initially, TYR can catalyze the oxidation of dopamine to dopaminechrome, which can efficiently quench the fluorescence intensity of AuNCs at 630nm based on dynamic quenching process...
November 25, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28002417/predicting-rift-valley-fever-inter-epidemic-activities-and-outbreak-patterns-insights-from-a-stochastic-host-vector-model
#4
Sansao A Pedro, Shirley Abelman, Henri E Z Tonnang
Rift Valley fever (RVF) outbreaks are recurrent, occurring at irregular intervals of up to 15 years at least in East Africa. Between outbreaks disease inter-epidemic activities exist and occur at low levels and are maintained by female Aedes mcintoshi mosquitoes which transmit the virus to their eggs leading to disease persistence during unfavourable seasons. Here we formulate and analyse a full stochastic host-vector model with two routes of transmission: vertical and horizontal. By applying branching process theory we establish novel relationships between the basic reproduction number, R0, vertical transmission and the invasion and extinction probabilities...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27988227/methods-for-the-analysis-of-early-oogenesis-in-zebrafish
#5
Yaniv M Elkouby, Mary C Mullins
Oocyte differentiation is a highly dynamic and intricate developmental process whose mechanistic understanding advances female reproduction, fertility, and ovarian cancer biology. Despite the many attributes of the zebrafish model, it has yet to be fully exploited for the investigation of early oocyte differentiation and ovarian development. This is partly because the properties of the adult zebrafish ovary make it technically challenging to access early stage oocytes. As a result, characterization of these stages has been lacking and tools for their analysis have been insufficient...
December 14, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27941611/transcriptional-response-of-silkworm-bombyx-mori-eggs-to-o%C3%A2-or-hcl-treatment
#6
Jing Gong, Sha Tian, Xia Zhou, Huan Yang, Yong Zhu, Yong Hou
Diapause is a common biological phenomenon that occurs in many organisms, including fish, insects, and nematodes. In the silkworm (Bombyx mori), diapause generally occurs in the egg stage. Treatment with O₂, HCl, or other compounds can prevent egg diapause. Here, we characterized the transcriptomic responses of newly laid eggs treated with O₂ or HCl. Digital gene expression analysis showed that 610 genes in O₂-treated eggs and 656 in HCl-treated eggs were differentially expressed. Of these, 343 genes were differentially expressed in both treatments...
December 7, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27921298/female-induced-remote-regulation-of-sperm-physiology-may-provide-opportunities-for-gamete-level-mate-choice
#7
Jukka Kekäläinen, Jonathan P Evans
In sedentary externally fertilizing species, direct interactions between mating partners are limited and prefertilization communication between sexes occurs largely at the gamete level. Certain combinations of eggs and sperm often have higher fertilization success than others, which may be contingent on egg-derived chemical factors that preferentially attract sperm from compatible males. Here, we examine the mechanisms underlying such effects in the marine mussel Mytilus galloprovincialis, where differential sperm attraction has recently been shown to be associated with variation in offspring viability...
December 6, 2016: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/27918611/moisture-absorption-and-water-dynamics-in-the-powder-of-egg-albumen-peptide-met-pro-asp-ala-his-leu
#8
Shuailing Yang, Xuye Liu, Mingdi Zhang, Songyi Lin, Feng Chen
Moisture absorbed into the powder of Met-Pro-Asp-Ala-His-Leu (MPDAHL)-a novel egg albumen antioxidant peptide-profoundly affects its properties. In this study, we elucidated water dynamics in MPDAHL using DVS, DSC, and low-field (1) H NMR. Based on the DVS data, we found that MPDAHL sorption kinetics obey a parallel exponential model. DSC results indicated that both water and heating could change the microstructure of MPDAHL. The T2 parameters of NMR reflected the different phases of moisture absorption revealed that there were 4 categories of water with different states or mobility in the MPDAHL during the moisture absorption process...
January 2017: Journal of Food Science
https://www.readbyqxmd.com/read/27911710/eml-proteins-in-microtubule-regulation-and-human-disease
#9
REVIEW
Andrew M Fry, Laura O'Regan, Jessica Montgomery, Rozita Adib, Richard Bayliss
The EMLs are a conserved family of microtubule-associated proteins (MAPs). The founding member was discovered in sea urchins as a 77-kDa polypeptide that co-purified with microtubules. This protein, termed EMAP for echinoderm MAP, was the major non-tubulin component present in purified microtubule preparations made from unfertilized sea urchin eggs [J. Cell Sci. (1993) 104: , 445-450; J. Cell Sci. (1987) 87: (Pt 1), 71-84]. Orthologues of EMAP were subsequently identified in other echinoderms, such as starfish and sand dollar, and then in more distant eukaryotes, including flies, worms and vertebrates, where the name of ELP or EML (both for EMAP-like protein) has been adopted [BMC Dev...
October 15, 2016: Biochemical Society Transactions
https://www.readbyqxmd.com/read/27894513/complement-in-the-fundamental-processes-of-the-cell
#10
Owen A Hawksworth, Liam G Coulthard, Trent M Woodruff
Once regarded solely as an activator of innate immunity, it is now clear that the complement system acts in an assortment of cells and tissues, with immunity only one facet of a diverse array of functions under the influence of the complement proteins. Throughout development, complement activity has now been demonstrated from early sperm-egg interactions in fertilisation, to regulation of epiboly and organogenesis, and later in refinement of cerebral synapses. Complement has also been shown to regulate homeostasis of adult tissues, controlling cell processes such as migration, survival, repair, and regeneration...
November 25, 2016: Molecular Immunology
https://www.readbyqxmd.com/read/27886633/a-novel-method-to-harvest-chlorella-sp-via-low-cost-bioflocculant-influence-of-temperature-with-kinetic-and-thermodynamic-functions
#11
Richa Kothari, Vinayak V Pathak, Arya Pandey, Shamshad Ahmad, Chandni Srivastava, V V Tyagi
In this study, harvesting efficiency (HE) of bioflocculant (egg shell) was observed with variation in flocculent concentrations (0-100mgL(-1)), temperature (30°C, 35°C 40°C, 45°C and 50°C) and variable contact time (0-50min). It was found maximum (≈95.6%) with 100mgL(-1) bioflocculant concentration whereas influence of temperature was also observed with optimized concentration of bioflocculant (100mgL(-1)) at 40°C (≈98.1%) and 50°C (≈99.3%), in 30min of contact time. Significant changes in algal cell structures were also analyzed after exposure to various temperatures with microscopy, SEM (Scanning electron microscopy) and EDS (Energy dispersive X-ray spectroscopy) images with and without bioflocculant...
February 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27878214/lethal-and-sublethal-effects-of-essential-oil-of-lippia-sidoides-verbenaceae-and-monoterpenes-on-chagas-disease-vector-rhodnius-prolixus
#12
Marcela B Figueiredo, Geovany A Gomes, Jayme M Santangelo, Emerson G Pontes, Patricia Azambuja, Elói S Garcia, Mário G de Carvalho
The aim of this study was to identify the composition of the essential oil from leaves of Lippia sidoides (EOLS), a typical shrub commonly found in the dry northeast of Brazil, popularly known as "alecrim-pimenta". Additionally, we investigated the nymphicidal, ovicidal, phagoinhibitory and excretion effects of EOLS, its major constituent thymol and its isomer carvacrol, on fourth instar nymphs and eggs of Rhodnius prolixus, the Chagas' disease vector. The nymphicidal and ovicidal activity of thymol, carvacrol, and EOLS was assessed by tests using impregnated Petri dishes...
January 1, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/27805357/facile-synthesis-of-three-dimensional-heteroatom-doped-and-hierarchical-egg-box-like-carbons-derived-from-moringa-oleifera-branches-for-high-performance-supercapacitors
#13
Yijin Cai, Ying Luo, Yong Xiao, Xiao Zhao, Yeru Liang, Hang Hu, Hanwu Dong, Luyi Sun, Yingliang Liu, Mingtao Zheng
In this paper, we demonstrate that Moringa oleifera branches, a renewable biomass waste with abundant protein content, can be employed as novel precursor to synthesize three-dimensional heteroatom-doped and hierarchical egg-box-like carbons (HEBLCs) by a facile room-temperature pretreatment and direct pyrolysis process. The as-prepared HEBLCs possess unique egg-box-like frameworks, high surface area, and interconnected porosity as well as the doping of heteroatoms (oxygen and nitrogen), endowing its excellent electrochemical performances (superior capacity, high rate capability, and outstanding cycling stability)...
December 7, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27788573/optimized-methods-for-the-isolation-of-arabidopsis-female-central-cells-and-their-nuclei
#14
Kyunghyuk Park, Jennifer M Frost, Adam James Adair, Dong Min Kim, Hyein Yun, Janie S Brooks, Robert L Fischer, Yeonhee Choi
The Arabidopsis female gametophyte contains seven cells with eight haploid nuclei buried within layers of sporophytic tissue. Following double fertilization, the egg and central cells of the gametophyte develop into the embryo and endosperm of the seed, respectively. The epigenetic status of the central cell has long presented an enigma due both to its inaccessibility, and the fascinating epigenome of the endosperm, thought to have been inherited from the central cell following activity of the DEMETER demethylase enzyme, prior to fertilization...
October 2016: Molecules and Cells
https://www.readbyqxmd.com/read/27788149/transcriptional-profiling-of-egg-allergy-and-relationship-to-disease-phenotype
#15
Roman Kosoy, Charuta Agashe, Alexander Grishin, Donald Y Leung, Robert A Wood, Scott H Sicherer, Stacie M Jones, A Wesley Burks, Wendy F Davidson, Robert W Lindblad, Peter Dawson, Miriam Merad, Brian A Kidd, Joel T Dudley, Hugh A Sampson, M Cecilia Berin
BACKGROUND: Egg allergy is one of the most common food allergies of childhood. There is a lack of information on the immunologic basis of egg allergy beyond the role of IgE. OBJECTIVE: To use transcriptional profiling as a novel approach to uncover immunologic processes associated with different phenotypes of egg allergy. METHODS: Peripheral blood mononuclear cells (PBMCs) were obtained from egg-allergic children who were defined as reactive (BER) or tolerant (BET) to baked egg, and from food allergic controls (AC) who were egg non-allergic...
2016: PloS One
https://www.readbyqxmd.com/read/27781291/expressed-microrna-associated-with-high-rate-of-egg-production-in-chicken-ovarian-follicles
#16
N Wu, U Gaur, Q Zhu, B Chen, Z Xu, X Zhao, M Yang, D Li
MicroRNA (miRNA) is a highly conserved class of small noncoding RNA about 19-24 nucleotides in length that function in a specific manner to post-transcriptionally regulate gene expression in organisms. Tissue miRNA expression studies have discovered a myriad of functions for miRNAs in various aspects, but a role for miRNAs in chicken ovarian tissue at 300 days of age has not hitherto been reported. In this study, we performed the first miRNA analysis of ovarian tissues in chickens with low and high rates of egg production using high-throughput sequencing...
October 26, 2016: Animal Genetics
https://www.readbyqxmd.com/read/27768087/delivery-of-nucleic-acids-through-embryo-microinjection-in-the-worldwide-agricultural-pest-insect-ceratitis-capitata
#17
Paolo Gabrieli, Francesca Scolari
The Mediterranean fruit fly (medfly) Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) is a pest species with extremely high agricultural relevance. This is due to its reproductive behavior: females damage the external surface of fruits and vegetables when they lay eggs and the hatched larvae feed on their pulp. Wild C. capitata populations are traditionally controlled through insecticide spraying and/or eco-friendly approaches, the most successful being the Sterile Insect Technique (SIT). The SIT relies on mass-rearing, radiation-based sterilization and field release of males that retain their capacity to mate but are not able to generate fertile progeny...
October 1, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27717305/a-novel-method-for-quantifying-the-rate-of-embryogenesis-uncovers-considerable-genetic-variation-for-the-duration-of-embryonic-development-in-drosophila-melanogaster
#18
Barbara Horváth, Andrea J Betancourt, Alex T Kalinka
BACKGROUND: Embryogenesis is a highly conserved, canalized process, and variation in the duration of embryogenesis (DOE), i.e., time from egg lay to hatching, has a potentially profound effect on the outcome of within- and between-species competition. There is both intra- and inter-specific variation in this trait, which may provide important fuel for evolutionary processes, particularly adaptation. However, while genetic variation underlying simpler morphological traits, or with large phenotypic effects is well described in the literature, less is known about the underlying genetics of traits, such as DOE, partly due to a lack of tools with which to study them...
October 7, 2016: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/27609093/molecular-characterization-and-expression-analysis-of-three-novel-autophagy-related-genes-from-the-cattle-tick-rhipicephalus-boophilus-microplus-acari-ixodidae
#19
José Miguel Flores Fernández, Carla Patricia Barragán Álvarez, Carla Vanessa Sánchez Hernández, Eduardo Padilla Camberos, Celia González Castillo, Daniel Ortuño Sahagún, Moisés Martínez Velázquez
The cattle tick Rhipicephalus (Boophilus) microplus is a hematophagous ectoparasite of major importance for the livestock industry. It shows a remarkable ability to survive over long periods without feeding. However, the mechanisms used to endure long-term starvation are poorly understood. It is believed that autophagy, a process of intracellular protein degradation, may play a significant role to confront adverse environmental conditions. To advance our understanding of autophagy in R. microplus, in the present study we report the molecular characterization of three autophagy-related (ATG) genes, namely, RmATG3, RmATG4 and RmATG6, as well as their expression profiles in different developmental stages and organs of the parasite...
November 2016: Parasitology
https://www.readbyqxmd.com/read/27603331/minimally-processed-functional-foods-technological-and-operational-pathways
#20
Svetlana Rodgers
This paper offers a concise review of technical and operational concepts underpinning commercialization of minimally processed functional foods (FFs), foods with fresh-like qualities commanding premium prices. The growing number of permitted nutritional content/health claims, many of which relate to well-being, coupled with emerging extraction and food processing technologies offers new exciting opportunities for small and medium size enterprises (SMEs) specializing in fresh produce to play an active role in the health market...
October 2016: Journal of Food Science
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