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https://www.readbyqxmd.com/read/29096248/retrospective-proteomic-analysis-of-a-novel-cancer-metastasis-promoting-rgd-containing-peptide
#1
Michiyo Tsuru, Michio Sata, Maki Tanaka, Hideaki Umeyama, Yoshio Kodera, Mieko Shiwa, Norikazu Aoyagi, Kaori Yasuda, Kei Matsuoka, Takaaki Fukuda, Hideaki Yamana, Kensei Nagata
Patients who undergo surgical extirpation of a primary liver carcinoma followed by radiotherapy and chemotherapy leading to complete remission are nevertheless known to develop cancerous metastases 3-10 years later. We retrospectively examined the blood sera collected over 8 years from 30 patients who developed bone metastases after the complete remission of liver cancer to identify serum proteins showing differential expression compared to patients without remission. We detected a novel RGD (Arg-Gly-Asp)-containing peptide derived from the C-terminal portion of fibrinogen in the sera of metastatic patients that appeared to control the EMT (epithelial-mesenchymal transition) of cancer cells, in a process associated with miR-199a-3p...
December 2017: Translational Oncology
https://www.readbyqxmd.com/read/29053871/cellular-and-morphological-characterization-of-blastoderms-from-freshly-laid-broiler-eggs
#2
N Pokhrel, E Ben-Tal Cohen, O Genin, D Sela-Donenfeld, Y Cinnamon
The pioneering study of Eyal-Giladi and Kochav (EG&K; Eyal-Giladi and Kochav, 1976) on the early developmental stages-from fertilization, through oviposition, to the gastrulation process-set the standard for characterizing chicken embryos, and has been used in numerous studies over the years. During uterine development, the chicken embryo undergoes dramatic changes, extremely rapid cell cycles, massive cell death, and axial determination processes. However, once the egg is laid, the temperature drops and the embryo enters into a diapause-like state...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29034438/the-pathways-fertility-preservation-decision-aid-website-for-women-with-cancer-development-and-field-testing
#3
Terri L Woodard, Aubri S Hoffman, Laura A Covarrubias, Deborah Holman, Leslie Schover, Andrea Bradford, Derek B Hoffman, Aakrati Mathur, Jerah Thomas, Robert J Volk
PURPOSE: To improve survivors' awareness and knowledge of fertility preservation counseling and treatment options, this study engaged survivors and providers to design, develop, and field-test Pathways: a fertility preservation patient decision aid website for young women with cancer©. METHODS: Using an adapted user-centered design process, our stakeholder advisory group and research team designed and optimized the Pathways patient decision aid website through four iterative cycles of review and revision with clinicians (n = 21) and survivors (n = 14)...
October 15, 2017: Journal of Cancer Survivorship: Research and Practice
https://www.readbyqxmd.com/read/28992814/field-efficacy-and-safety-of-fluralaner-solution-for-administration-in-drinking-water-for-the-treatment-of-poultry-red-mite-dermanyssus-gallinae-infestations-in-commercial-flocks-in-europe
#4
Emmanuel Thomas, Mathieu Chiquet, Björn Sander, Eva Zschiesche, Annie Sigognault Flochlay
BACKGROUND: Welfare concerns, production losses caused by Dermanyssus gallinae, the poultry red mite (PRM), and widespread mite resistance to environmentally applied acaricides continue to drive an urgent need for new and effective control measures. Fluralaner is a novel systemic acaricide developed to address that need. A series of field studies was initiated to investigate the safety and efficacy of a fluralaner solution (10 mg/ml) administered in drinking water at a dose rate of 0...
October 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28917743/selection-of-an-aptamer-against-muscovy-duck-parvovirus-for-highly-sensitive-rapid-visual-detection-by-label-free-aptasensor
#5
Taofeng Lu, Qin Ma, Wenzhuo Yan, Yuanzhi Wang, Yuanyuan Zhang, Lili Zhao, Hongyan Chen
Muscovy duck parvovirus (MDPV) causes high mortality and morbidity in ducks. This study investigated a novel aptamer-based, label-free aptasensor detection of MDPV. In this study, we developed an ssDNA aptamer using the filtration partition and lambda exonuclease method with an affinity-based monitor and counter-screening process. After 15 rounds of SELEX (systematic evolution of ligands by exponential enrichment), the ssDNA aptamer Apt-10, which specifically bound to MDPV with high affinity (Kd = 467nM) was successfully screened, and the aptamer was also found to be good specific to MDPV...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28916657/tenascin-c-and-integrin-%C3%AE-9-mediate-interactions-of-prostate-cancer-with-the-bone-microenvironment
#6
Rebeca San Martin, Ravi Pathak, Antrix Jain, Sung Yun Jung, Susan G Hilsenbeck, María C Piña-Barba, Andrew G Sikora, Kenneth J Pienta, David R Rowley
Deposition of the extracellular matrix protein tenascin-C is part of the reactive stroma response, which has a critical role in prostate cancer progression. Here, we report that tenascin C is expressed in the bone endosteum and is associated with formation of prostate bone metastases. Metastatic cells cultured on osteo-mimetic surfaces coated with tenascin C exhibited enhanced adhesion and colony formation as mediated by integrin α9β1. In addition, metastatic cells preferentially migrated and colonized tenascin-C-coated trabecular bone xenografts in a novel system that employed chorioallantoic membranes of fertilized chicken eggs as host...
September 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28916030/fellowship-candidate-selection-at-the-best-medical-center-a-novel-process
#7
Philip R Cohen
Dr. Ida Lystic completed her MD degree at the prestigious Harvey Medical School (which has since been renamed the Harvey Provider School) and her residency in internal medicine and gastroenterology fellowship at the OTHER (Owen T. Henry and Eugene Rutherford) Medical Center. She was subsequently hired as an assistant professor at the BEST (Byron Edwards and Samuel Thompson) Medical Center in 2015. After eventually completing an extensive list of employment requirements, she was able to begin seeing patients...
September 2017: Clinics in Dermatology
https://www.readbyqxmd.com/read/28901845/a-review-of-patents-on-therapeutic-potential-and-delivery-of-hydrogen-sulfide
#8
Richa Verma, Yumna Akhtar, Somnath Singh
Hydrogen sulfide (H2S) is a colorless gas with a characteristic smell of rotten eggs. Once only thought of as a toxic gas, evidence now shows that H2S plays major roles in pathological and physiological activities. These roles are being utilized to treat diseases and disorders ranging from hypertension, inflammation, edema, cardiovascular issues, chronic pain, cancer, and many more. Challenges facing the use of H2S currently involve achieving the optimum therapeutic concentrations, synthesizing chemically and physiologically stable donors, and developing clinically appropriate delivery systems...
September 11, 2017: Recent Patents on Drug Delivery & Formulation
https://www.readbyqxmd.com/read/28886878/divergently-expressed-gene-identification-and-interaction-prediction-of-long-noncoding-rna-and-mrna-involved-in-duck-reproduction
#9
Jindong Ren, Xue Du, Tao Zeng, Li Chen, Junda Shen, Lizhi Lu, Jianhong Hu
Long noncoding RNAs (lncRNAs) and divergently expressed genes exist widely in different tissues of mammals and birds, in which they are involved in various biological processes. However, there is limited information on their role in the regulation of normal biological processes during differentiation, development, and reproduction in birds. In this study, whole transcriptome strand-specific RNA sequencing of the ovary from young ducks (60days), first-laying ducks (160days), and old ducks, i.e., ducks that stopped laying eggs (490days) was performed...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28884645/the-chicken-and-egg-development-of-political-opinionsthe-roles-of-genes-social-status-ideology-and-information
#10
Beattie J D Peter
Twin studies have revealed political ideology to be partially heritable. Neurological research has shown that ideological differences are reflected in brain structure and response, suggesting a direct genotype-phenotype link. Social and informational environments, however, also demonstrably affect brain structure and response. This leads to a "chicken-and-egg" question: do genes produce brains with ideological predispositions, causing the preferential absorption of consonant information and thereby forming an ideology, or do social and informational environments do most of the heavy lifting, with genetic evidence the spurious artifact of outdated methodology? Or are both inextricably intertwined contributors? This article investigates the relative contributions of genetic and environmental factors to ideological development using a role-play experiment investigating the development of opinions on a novel political issue...
2017: Politics and the Life Sciences: the Journal of the Association for Politics and the Life Sciences
https://www.readbyqxmd.com/read/28880872/microtubule-actin-crosslinking-factor-1-macf1-domain-function-in-balbiani-body-dissociation-and-nuclear-positioning
#11
Matias Escobar-Aguirre, Hong Zhang, Allison Jamieson-Lucy, Mary C Mullins
Animal-vegetal (AV) polarity of most vertebrate eggs is established during early oogenesis through the formation and disassembly of the Balbiani Body (Bb). The Bb is a structure conserved from insects to humans that appears as a large granule, similar to a mRNP granule composed of mRNA and proteins, that in addition contains mitochondria, ER and Golgi. The components of the Bb, which have amyloid-like properties, include germ cell and axis determinants of the embryo that are anchored to the vegetal cortex upon Bb disassembly...
September 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28794984/seasonality-richness-and-prevalence-of-intestinal-parasites-of-three-neotropical-primates-alouatta-seniculus-ateles-hybridus-and-cebus-versicolor-in-a-fragmented-forest-in-colombia
#12
Silvia Rondón, Mario Ortiz, Cielo León, Nelson Galvis, Andrés Link, Camila González
Studies on parasites infecting non-human primates are essential to better understand the potential threat to humans of zoonoses transmission, particularly under the current processes of pervasive land use change and biodiversity loss. The natural ecosystems in the Middle Magdalena river basin in Colombia have suffered a dramatic reduction and transformation into pastures and agroindustrial monocultures, threatening their biodiversity, and probably affecting the dynamics between parasites and their hosts, as well as altering the disease transmission cycles between wild populations and humans...
December 2017: International Journal for Parasitology. Parasites and Wildlife
https://www.readbyqxmd.com/read/28765643/mir-202-5p-is-a-novel-germ-plasm-specific-microrna-in-zebrafish
#13
Jing Zhang, Wei Liu, Yilin Jin, Peng Jia, Kuntong Jia, Meisheng Yi
Gametogenesis is a complicated biological process by which sperm and egg are produced for genetic transmission between generations. In many animals, the germline is segregated from the somatic lineage in early embryonic development through the specification of primordial germ cells (PGCs), the precursors of gametes for reproduction and fertility. In some species, such as fruit fly and zebrafish, PGCs are determined by the maternally provided germ plasm which contains various RNAs and proteins. Here, we identified a germ plasm/PGC-specific microRNA miR-202-5p for the first time in zebrafish...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747474/male-female-relatedness-at-specific-snp-linkage-groups-influences-cryptic-female-choice-in-chinook-salmon-oncorhynchus-tshawytscha
#14
Cornelia Geßner, Sheri L Johnson, Paul Fisher, Shannon Clarke, Kim Rutherford, Jane Symonds, Neil J Gemmell
In a range of taxa, the relatedness between mates influences both pre- and post-mating processes of sexual selection. However, relatively little is known about the genetic loci facilitating such a bias, with the exception of the major histocompatibility complex. Here, we performed tightly controlled replicated in vitro fertilization trials to explore the impact of relatedness on two possible mechanisms of cryptic female choice (CFC) in Chinook salmon (Oncorhynchus tshawytscha). We tested (i) whether relatedness of mates, assessed using 682 single nucleotide polymorphisms (SNPs) on 29 SNP-linkage groups (LGs), biases a male's sperm velocity in ovarian fluid (a parameter previously shown to predict male fertilization success), and (ii) whether relatedness of mates governs fertilization success via other mechanisms, probably via sperm-egg interactions...
July 26, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28740415/the-extracellular-matrix-of-eggshell-displays-anti-inflammatory-activities-through-nf-%C3%AE%C2%BAb-in-lps-triggered-human-immune-cells
#15
Tram T Vuong, Sissel B Rønning, Henri-Pierre Suso, Ralf Schmidt, Kristian Prydz, Marlene Lundström, Anders Moen, Mona E Pedersen
Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage on burned and cut skin injuries for >400 years in Asian countries, and is available in large quantities from egg industries. Our aim was to characterize ESM that was separated and processed from egg waste, and to study whether this material possesses anti-inflammatory properties, making it suitable as an ingredient in industrial production of low cost wound healing products. Our results show that the processed ESM particles retain a fibrous structure similar to that observed for the native membrane, and contain collagen, and carbohydrate components such as hyaluronic acid and sulfated glycosaminoglycans, as well as N-glycans, mostly with uncharged structures...
2017: Journal of Inflammation Research
https://www.readbyqxmd.com/read/28727079/detection-of-dietary-dna-protein-and-glyphosate-in-meat-milk-and-eggs
#16
A L Van Eenennaam, A E Young
Products such as meat, milk, and eggs from animals that have consumed genetically engineered (GE) feed are not currently subject to mandatory GE labeling requirements. Some voluntary "non-genetically modified organism" labeling has been associated with such products, indicating that the animals were not fed GE crops, as there are no commercialized GE food animals. This review summarizes the available scientific literature on the detection of dietary DNA and protein in animal products and briefly discusses the implications of mandatory GE labeling for products from animals that have consumed GE feed...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28715428/cip29-is-phosphorylated-following-activation-of-the-dna-damage-response-in-xenopus-egg-extracts
#17
Janet Holden, Elaine M Taylor, Howard D Lindsay
Acting through a complex signalling network, DNA lesions trigger a range of cellular responses including DNA repair, cell cycle arrest, altered gene expression and cell death, which help to limit the mutagenic effects of such DNA damage. RNA processing factors are increasingly being recognised as important targets of DNA damage signalling, with roles in the regulation of gene expression and also more directly in the promotion of DNA repair. In this study, we have used a Xenopus laevis egg extract system to analyse the DNA damage-dependent phosphorylation of a putative RNA export factor, Cip29...
2017: PloS One
https://www.readbyqxmd.com/read/28700597/differentially-expressed-micrornas-in-diapausing-versus-hcl-treated-bombyx-embryos
#18
Wentao Fan, Yangsheng Zhong, Mingyue Qin, Bimin Lin, Fangyan Chen, Huichao Yan, Wenchu Li, Jianrong Lin
Differentially expressed microRNAs were detected to explore the molecular mechanisms of diapause termination. The total small RNA of diapause-destined silkworm eggs and HCl-treated eggs was extracted and then sequenced using HiSeq high-throughput method. 44 novel miRNAs were discovered. Compared to those in the diapause-destined eggs, 61 miRNAs showed significant changes in the acid-treated eggs, with 23 being up-regulated and 38 being down-regulated. The potential target genes of differentially expressed miRNAs were predicted by miRanda...
2017: PloS One
https://www.readbyqxmd.com/read/28675377/investigation-of-novel-superparamagnetic-ni0-5zn0-5fe2o4-albumen-nanoparticles-for-controlled-delivery-of-anticancer-drug
#19
Mohd Qasim, Khushnuma Asghar, Gangappa Dharmapuri, D Das
In the present work, multifunctional Ni0.5Zn0.5Fe2O4@albumen (NZF@Alb) and doxorubicin-loaded Ni0.5Zn0.5Fe2O4@albumen (NZF@Alb-Dox) core-shell nanoparticles have been prepared by a green and simple method using inexpensive chicken egg albumen and have been characterized for different physiochemical properties. The structural, morphological, thermal, and magnetic properties of the prepared nanoparticles have been investigated by an x-ray diffractometer, high-resolution transmission electron microscopy (HRTEM), field emission scanning electron microscopy, Fourier-transformed infrared, thermogravimetric analysis, and vibrating sample magnetometer techniques...
July 4, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28663368/betaine-is-accumulated-via-transient-choline-dehydrogenase-activation-during-mouse-oocyte-meiotic-maturation
#20
COMPARATIVE STUDY
Taylor McClatchie, Megan Meredith, Mariame O Ouédraogo, Sandy Slow, Michael Lever, Mellissa R W Mann, Steven H Zeisel, Jacquetta M Trasler, Jay M Baltz
Betaine (N,N,N-trimethylglycine) plays key roles in mouse eggs and preimplantation embryos first in a novel mechanism of cell volume regulation and second as a major methyl donor in blastocysts, but its origin is unknown. Here, we determined that endogenous betaine was present at low levels in germinal vesicle (GV) stage mouse oocytes before ovulation and reached high levels in the mature, ovulated egg. However, no betaine transport into oocytes was detected during meiotic maturation. Because betaine can be synthesized in mammalian cells via choline dehydrogenase (CHDH; EC 1...
August 18, 2017: Journal of Biological Chemistry
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