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https://www.readbyqxmd.com/read/28335741/high-throughput-sequencing-of-pituitary-and-hypothalamic-microrna-transcriptome-associated-with-high-rate-of-egg-production
#1
Nan Wu, Qing Zhu, Binlong Chen, Jian Gao, Zhongxian Xu, Diyan Li
BACKGROUND: MicroRNAs exist widely in viruses, plants and animals. As endogenous small non-coding RNAs, miRNAs regulate a variety of biological processes. Tissue miRNA expression studies have discovered numerous functions for miRNAs in various tissues of chicken, but the regulation of miRNAs in chicken pituitary and hypothalamic development related to high and low egg-laying performance has remained unclear. RESULTS: In this study, using high-throughput sequencing technology, we sequenced two tissues (pituitary and hypothalamus) in 3 high- and 3 low-rate egg production Luhua chickens at the age of 300 days...
March 23, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28325896/egg-antigen-p40-of-schistosoma-japonicum-promotes-senescence-in-activated-hepatic-stellate-cells-via-skp2-p27-signaling-pathway
#2
Tianhua Xu, Jinling Chen, Dandan Zhu, Liuting Chen, Jianxin Wang, Xiaolei Sun, Bin Hu, Yinong Duan
Schistosomiasis is characterized by egg deposition, granulomatous inflammatory reaction and then subsequent hepatic fibrosis formation. Activated HSCs are regarded as the main effector cells in the progression of liver fibrosis and induction of senescence in hepatic stellate cells (HSCs) is vital to the reversion of hepatic fibrosis. Our previous work has showed that S. japonicum egg antigen p40 (Sjp40) could promote HSCs senescence via a STAT3/p53/p21 mechanism. In this paper, the major aim was to explore whether there are other signaling pathways in the process of Sjp40-induced HSCs aging and the underlying effect of SKP2/P27 signal pathway in this procedure...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28287085/dynamic-transcriptomes-identify-biogenic-amines-and-insect-like-hormonal-regulation-for-mediating-reproduction-in-schistosoma-japonicum
#3
Jipeng Wang, Ying Yu, Haimo Shen, Tao Qing, Yuanting Zheng, Qing Li, Xiaojin Mo, Shuqi Wang, Nana Li, Riyi Chai, Bin Xu, Mu Liu, Paul J Brindley, Donald P McManus, Zheng Feng, Leming Shi, Wei Hu
Eggs produced by the mature female parasite are responsible for the pathogenesis and transmission of schistosomiasis. Female schistosomes rely on a unique male-induced strategy to accomplish reproductive development, a process that is incompletely understood. Here we map detailed transcriptomic profiles of male and female Schistosoma japonicum across eight time points throughout the sexual developmental process from pairing to maturation. The dynamic gene expression pattern data reveal clear sex-related characteristics, indicative of an unambiguous functional division between males and females during their interplay...
March 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28212670/characterization-of-schistosoma-japonicum-cp1412-protein-as-a-novel-member-of-the-ribonuclease-t2-molecule-family-with-immune-regulatory-function
#4
Xue-Dan Ke, Shuang Shen, Li-Jun Song, Chuan-Xin Yu, Mihoko Kikuchi, Kenji Hirayama, Hong Gao, Jie Wang, Xuren Yin, Yuan Yao, Qian Liu, Wei Zhou
BACKGROUND: Schistosome infection typically induces a polarized Th2 type host immune response. As egg antigen molecules play key roles in this immunoregulatory process, clarifying their functions in schistosomiasis would facilitate the development of vaccine and immunotherapeutic methods. Schistosoma japonicum (Sj) CP1412 (GenBank: AY57074.1) has been identified as a new member of the RNase T2 family with immune regulatory functions. METHODS: The expression plasmid Sj CP1412-pET28a was constructed and transformed into bacteria for production of recombinant Sj CP1412 protein (rSj CP1412) via IPTG induction...
February 17, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28179521/egg-discrimination-along-a-gradient-of-natural-variation-in-eggshell-coloration
#5
Daniel Hanley, Tomáš Grim, Branislav Igic, Peter Samaš, Analía V López, Matthew D Shawkey, Mark E Hauber
Accurate recognition of salient cues is critical for adaptive responses, but the underlying sensory and cognitive processes are often poorly understood. For example, hosts of avian brood parasites have long been assumed to reject foreign eggs from their nests based on the total degree of dissimilarity in colour to their own eggs, regardless of the foreign eggs' colours. We tested hosts' responses to gradients of natural (blue-green to brown) and artificial (green to purple) egg colours, and demonstrate that hosts base rejection decisions on both the direction and degree of colour dissimilarity along the natural, but not artificial, gradient of egg colours...
February 8, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28171788/fabrication-of-a-novel-hemin-based-monolithic-column-and-its-application-in-separation-of-protein-from-complex-bio-matrix
#6
Xiaoya Jiang, Doudou Zhang, Xueying Li, Xixi Wang, Ligai Bai, Haiyan Liu, Hongyuan Yan
A novel polymer-based monolithic column was prepared via redox initiation system within the confines of a stainless steel column with 4.6mm i.d. In the processes, hemin and lauryl methacrylate were used as co-monomers; ethylene dimethacrylate as crosslinking agent; n-butyl alcohol, ethanediol, and N, N-dimethylformamide as tri-porogens; benzoyl peroxide and N, N-dimethyl aniline as redox initiation system. The resulting polymer-based monolithic columns were characterized by scanning electron microscopy, nitrogen adsorption-desorption instrument, and mercury intrusion porosimeter, respectively...
January 19, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28091611/multi-response-optimization-of-artemia-hatching-process-using-split-split-plot-design-based-response-surface-methodology
#7
V V Arun, Neelam Saharan, V Ramasubramanian, A M Babitha Rani, K R Salin, Ravindra Sontakke, Harsha Haridas, Deepak George Pazhayamadom
A novel method, BBD-SSPD is proposed by the combination of Box-Behnken Design (BBD) and Split-Split Plot Design (SSPD) which would ensure minimum number of experimental runs, leading to economical utilization in multi- factorial experiments. The brine shrimp Artemia was tested to study the combined effects of photoperiod, temperature and salinity, each with three levels, on the hatching percentage and hatching time of their cysts. The BBD was employed to select 13 treatment combinations out of the 27 possible combinations that were grouped in an SSPD arrangement...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28086873/cecal-micrornaome-response-to-salmonella-enterica-serovar-enteritidis-infection-in-white-leghorn-layer
#8
Guixian Wu, Yukai Qi, Xiaoyi Liu, Ning Yang, Guiyun Xu, Liying Liu, Xianyao Li
BACKGROUND: Salmonella enterica serovar Enteritidis (SE) is a food-borne pathogen and of great threat to human health through consuming the contaminated poultry products. MicroRNAs (miRNAs) play an important role in different biological activities and have been shown to regulate the innate immunity in the bacterial infection. The objective of this study is to identify miRNAs associated with SE infection in laying chicken cecum. RESULTS: Average number of reads of three libraries constructed from infected and non-infected chickens was 12,476,156 and 10,866,976, respectively...
January 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28017550/a-novel-bioconversion-for-value-added-products-from-food-waste-using-musca-domestica
#9
Yi Niu, Dong Zheng, Binghua Yao, Zizhe Cai, Zhimin Zhao, Shengqing Wu, Peiqing Cong, Depo Yang
Food waste, as a major part of the municipal solid waste has been generated increasingly worldwide. Efficient and feasible utilization of this waste material for productivity process is significant for both economical and environmental reasons. In the present study, Musca domestica larva was used as the carrier to conduct a bioconversion with food waste to get the value-added maggot protein, oil and organic fertilizers. Methods of adult flies rearing, culture medium adjuvant selection, maggot culture conditions, stocking density and the valorization of the waste have been explored...
December 22, 2016: Waste Management
https://www.readbyqxmd.com/read/28012319/a-novel-fluorimetric-sensing-platform-for-highly-sensitive-detection-of-organophosphorus-pesticides-by-using-egg-white-encapsulated-gold-nanoclusters
#10
Xu Yan, Hongxia Li, Tianyu Hu, Xingguang Su
Assays for organophosphorus pesticides (OPs) with high sensitivity as well as on-site screening have been urgently required to protect ecosystem and prevent disease. Herein, a novel fluorimetric sensing platform was constructed for quantitative detection of OPs via tyrosinase (TYR) enzyme-controlled quenching of gold nanoclusters (AuNCs). One-step green synthetic approach was developed for the synthesis of AuNCs by using chicken egg white (CEW) as template and stabilizer. Initially, TYR can catalyze the oxidation of dopamine to dopaminechrome, which can efficiently quench the fluorescence intensity of AuNCs at 630nm based on dynamic quenching process...
May 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28002417/predicting-rift-valley-fever-inter-epidemic-activities-and-outbreak-patterns-insights-from-a-stochastic-host-vector-model
#11
Sansao A Pedro, Shirley Abelman, Henri E Z Tonnang
Rift Valley fever (RVF) outbreaks are recurrent, occurring at irregular intervals of up to 15 years at least in East Africa. Between outbreaks disease inter-epidemic activities exist and occur at low levels and are maintained by female Aedes mcintoshi mosquitoes which transmit the virus to their eggs leading to disease persistence during unfavourable seasons. Here we formulate and analyse a full stochastic host-vector model with two routes of transmission: vertical and horizontal. By applying branching process theory we establish novel relationships between the basic reproduction number, R0, vertical transmission and the invasion and extinction probabilities...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27988227/methods-for-the-analysis-of-early-oogenesis-in-zebrafish
#12
Yaniv M Elkouby, Mary C Mullins
Oocyte differentiation is a highly dynamic and intricate developmental process whose mechanistic understanding advances female reproduction, fertility, and ovarian cancer biology. Despite the many attributes of the zebrafish model, it has yet to be fully exploited for the investigation of early oocyte differentiation and ovarian development. This is partly because the properties of the adult zebrafish ovary make it technically challenging to access early stage oocytes. As a result, characterization of these stages has been lacking and tools for their analysis have been insufficient...
December 14, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27941611/transcriptional-response-of-silkworm-bombyx-mori-eggs-to-o%C3%A2-or-hcl-treatment
#13
Jing Gong, Sha Tian, Xia Zhou, Huan Yang, Yong Zhu, Yong Hou
Diapause is a common biological phenomenon that occurs in many organisms, including fish, insects, and nematodes. In the silkworm (Bombyx mori), diapause generally occurs in the egg stage. Treatment with O₂, HCl, or other compounds can prevent egg diapause. Here, we characterized the transcriptomic responses of newly laid eggs treated with O₂ or HCl. Digital gene expression analysis showed that 610 genes in O₂-treated eggs and 656 in HCl-treated eggs were differentially expressed. Of these, 343 genes were differentially expressed in both treatments...
December 7, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27921298/female-induced-remote-regulation-of-sperm-physiology-may-provide-opportunities-for-gamete-level-mate-choice
#14
Jukka Kekäläinen, Jonathan P Evans
In sedentary externally fertilizing species, direct interactions between mating partners are limited and prefertilization communication between sexes occurs largely at the gamete level. Certain combinations of eggs and sperm often have higher fertilization success than others, which may be contingent on egg-derived chemical factors that preferentially attract sperm from compatible males. Here, we examine the mechanisms underlying such effects in the marine mussel Mytilus galloprovincialis, where differential sperm attraction has recently been shown to be associated with variation in offspring viability...
December 6, 2016: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/27918611/moisture-absorption-and-water-dynamics-in-the-powder-of-egg-albumen-peptide-met-pro-asp-ala-his-leu
#15
Shuailing Yang, Xuye Liu, Mingdi Zhang, Songyi Lin, Feng Chen
Moisture absorbed into the powder of Met-Pro-Asp-Ala-His-Leu (MPDAHL)-a novel egg albumen antioxidant peptide-profoundly affects its properties. In this study, we elucidated water dynamics in MPDAHL using DVS, DSC, and low-field (1) H NMR. Based on the DVS data, we found that MPDAHL sorption kinetics obey a parallel exponential model. DSC results indicated that both water and heating could change the microstructure of MPDAHL. The T2 parameters of NMR reflected the different phases of moisture absorption revealed that there were 4 categories of water with different states or mobility in the MPDAHL during the moisture absorption process...
January 2017: Journal of Food Science
https://www.readbyqxmd.com/read/27911710/eml-proteins-in-microtubule-regulation-and-human-disease
#16
REVIEW
Andrew M Fry, Laura O'Regan, Jessica Montgomery, Rozita Adib, Richard Bayliss
The EMLs are a conserved family of microtubule-associated proteins (MAPs). The founding member was discovered in sea urchins as a 77-kDa polypeptide that co-purified with microtubules. This protein, termed EMAP for echinoderm MAP, was the major non-tubulin component present in purified microtubule preparations made from unfertilized sea urchin eggs [J. Cell Sci. (1993) 104: , 445-450; J. Cell Sci. (1987) 87: (Pt 1), 71-84]. Orthologues of EMAP were subsequently identified in other echinoderms, such as starfish and sand dollar, and then in more distant eukaryotes, including flies, worms and vertebrates, where the name of ELP or EML (both for EMAP-like protein) has been adopted [BMC Dev...
October 15, 2016: Biochemical Society Transactions
https://www.readbyqxmd.com/read/27894513/complement-in-the-fundamental-processes-of-the-cell
#17
Owen A Hawksworth, Liam G Coulthard, Trent M Woodruff
Once regarded solely as an activator of innate immunity, it is now clear that the complement system acts in an assortment of cells and tissues, with immunity only one facet of a diverse array of functions under the influence of the complement proteins. Throughout development, complement activity has now been demonstrated from early sperm-egg interactions in fertilisation, to regulation of epiboly and organogenesis, and later in refinement of cerebral synapses. Complement has also been shown to regulate homeostasis of adult tissues, controlling cell processes such as migration, survival, repair, and regeneration...
April 2017: Molecular Immunology
https://www.readbyqxmd.com/read/27886633/a-novel-method-to-harvest-chlorella-sp-via-low-cost-bioflocculant-influence-of-temperature-with-kinetic-and-thermodynamic-functions
#18
Richa Kothari, Vinayak V Pathak, Arya Pandey, Shamshad Ahmad, Chandni Srivastava, V V Tyagi
In this study, harvesting efficiency (HE) of bioflocculant (egg shell) was observed with variation in flocculent concentrations (0-100mgL(-1)), temperature (30°C, 35°C 40°C, 45°C and 50°C) and variable contact time (0-50min). It was found maximum (≈95.6%) with 100mgL(-1) bioflocculant concentration whereas influence of temperature was also observed with optimized concentration of bioflocculant (100mgL(-1)) at 40°C (≈98.1%) and 50°C (≈99.3%), in 30min of contact time. Significant changes in algal cell structures were also analyzed after exposure to various temperatures with microscopy, SEM (Scanning electron microscopy) and EDS (Energy dispersive X-ray spectroscopy) images with and without bioflocculant...
February 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27878214/lethal-and-sublethal-effects-of-essential-oil-of-lippia-sidoides-verbenaceae-and-monoterpenes-on-chagas-disease-vector-rhodnius-prolixus
#19
Marcela B Figueiredo, Geovany A Gomes, Jayme M Santangelo, Emerson G Pontes, Patricia Azambuja, Elói S Garcia, Mário G de Carvalho
The aim of this study was to identify the composition of the essential oil from leaves of Lippia sidoides (EOLS), a typical shrub commonly found in the dry northeast of Brazil, popularly known as "alecrim-pimenta". Additionally, we investigated the nymphicidal, ovicidal, phagoinhibitory and excretion effects of EOLS, its major constituent thymol and its isomer carvacrol, on fourth instar nymphs and eggs of Rhodnius prolixus, the Chagas' disease vector. The nymphicidal and ovicidal activity of thymol, carvacrol, and EOLS was assessed by tests using impregnated Petri dishes...
January 1, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/27805357/facile-synthesis-of-three-dimensional-heteroatom-doped-and-hierarchical-egg-box-like-carbons-derived-from-moringa-oleifera-branches-for-high-performance-supercapacitors
#20
Yijin Cai, Ying Luo, Yong Xiao, Xiao Zhao, Yeru Liang, Hang Hu, Hanwu Dong, Luyi Sun, Yingliang Liu, Mingtao Zheng
In this paper, we demonstrate that Moringa oleifera branches, a renewable biomass waste with abundant protein content, can be employed as novel precursor to synthesize three-dimensional heteroatom-doped and hierarchical egg-box-like carbons (HEBLCs) by a facile room-temperature pretreatment and direct pyrolysis process. The as-prepared HEBLCs possess unique egg-box-like frameworks, high surface area, and interconnected porosity as well as the doping of heteroatoms (oxygen and nitrogen), endowing its excellent electrochemical performances (superior capacity, high rate capability, and outstanding cycling stability)...
December 7, 2016: ACS Applied Materials & Interfaces
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