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https://www.readbyqxmd.com/read/28794984/seasonality-richness-and-prevalence-of-intestinal-parasites-of-three-neotropical-primates-alouatta-seniculus-ateles-hybridus-and-cebus-versicolor-in-a-fragmented-forest-in-colombia
#1
Silvia Rondón, Mario Ortiz, Cielo León, Nelson Galvis, Andrés Link, Camila González
Studies on parasites infecting non-human primates are essential to better understand the potential threat to humans of zoonoses transmission, particularly under the current processes of pervasive land use change and biodiversity loss. The natural ecosystems in the Middle Magdalena river basin in Colombia have suffered a dramatic reduction and transformation into pastures and agroindustrial monocultures, threatening their biodiversity, and probably affecting the dynamics between parasites and their hosts, as well as altering the disease transmission cycles between wild populations and humans...
December 2017: International Journal for Parasitology. Parasites and Wildlife
https://www.readbyqxmd.com/read/28765643/mir-202-5p-is-a-novel-germ-plasm-specific-microrna-in-zebrafish
#2
Jing Zhang, Wei Liu, Yilin Jin, Peng Jia, Kuntong Jia, Meisheng Yi
Gametogenesis is a complicated biological process by which sperm and egg are produced for genetic transmission between generations. In many animals, the germline is segregated from the somatic lineage in early embryonic development through the specification of primordial germ cells (PGCs), the precursors of gametes for reproduction and fertility. In some species, such as fruit fly and zebrafish, PGCs are determined by the maternally provided germ plasm which contains various RNAs and proteins. Here, we identified a germ plasm/PGC-specific microRNA miR-202-5p for the first time in zebrafish...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747474/male-female-relatedness-at-specific-snp-linkage-groups-influences-cryptic-female-choice-in-chinook-salmon-oncorhynchus-tshawytscha
#3
Cornelia Geßner, Sheri L Johnson, Paul Fisher, Shannon Clarke, Kim Rutherford, Jane Symonds, Neil J Gemmell
In a range of taxa, the relatedness between mates influences both pre- and post-mating processes of sexual selection. However, relatively little is known about the genetic loci facilitating such a bias, with the exception of the major histocompatibility complex. Here, we performed tightly controlled replicated in vitro fertilization trials to explore the impact of relatedness on two possible mechanisms of cryptic female choice (CFC) in Chinook salmon (Oncorhynchus tshawytscha). We tested (i) whether relatedness of mates, assessed using 682 single nucleotide polymorphisms (SNPs) on 29 SNP-linkage groups (LGs), biases a male's sperm velocity in ovarian fluid (a parameter previously shown to predict male fertilization success), and (ii) whether relatedness of mates governs fertilization success via other mechanisms, probably via sperm-egg interactions...
July 26, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28740415/the-extracellular-matrix-of-eggshell-displays-anti-inflammatory-activities-through-nf-%C3%AE%C2%BAb-in-lps-triggered-human-immune-cells
#4
Tram T Vuong, Sissel B Rønning, Henri-Pierre Suso, Ralf Schmidt, Kristian Prydz, Marlene Lundström, Anders Moen, Mona E Pedersen
Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage on burned and cut skin injuries for >400 years in Asian countries, and is available in large quantities from egg industries. Our aim was to characterize ESM that was separated and processed from egg waste, and to study whether this material possesses anti-inflammatory properties, making it suitable as an ingredient in industrial production of low cost wound healing products. Our results show that the processed ESM particles retain a fibrous structure similar to that observed for the native membrane, and contain collagen, and carbohydrate components such as hyaluronic acid and sulfated glycosaminoglycans, as well as N-glycans, mostly with uncharged structures...
2017: Journal of Inflammation Research
https://www.readbyqxmd.com/read/28727079/detection-of-dietary-dna-protein-and-glyphosate-in-meat-milk-and-eggs
#5
A L Van Eenennaam, A E Young
Products such as meat, milk, and eggs from animals that have consumed genetically engineered (GE) feed are not currently subject to mandatory GE labeling requirements. Some voluntary "non-genetically modified organism" labeling has been associated with such products, indicating that the animals were not fed GE crops, as there are no commercialized GE food animals. This review summarizes the available scientific literature on the detection of dietary DNA and protein in animal products and briefly discusses the implications of mandatory GE labeling for products from animals that have consumed GE feed...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28715428/cip29-is-phosphorylated-following-activation-of-the-dna-damage-response-in-xenopus-egg-extracts
#6
Janet Holden, Elaine M Taylor, Howard D Lindsay
Acting through a complex signalling network, DNA lesions trigger a range of cellular responses including DNA repair, cell cycle arrest, altered gene expression and cell death, which help to limit the mutagenic effects of such DNA damage. RNA processing factors are increasingly being recognised as important targets of DNA damage signalling, with roles in the regulation of gene expression and also more directly in the promotion of DNA repair. In this study, we have used a Xenopus laevis egg extract system to analyse the DNA damage-dependent phosphorylation of a putative RNA export factor, Cip29...
2017: PloS One
https://www.readbyqxmd.com/read/28700597/differentially-expressed-micrornas-in-diapausing-versus-hcl-treated-bombyx-embryos
#7
Wentao Fan, Yangsheng Zhong, Mingyue Qin, Bimin Lin, Fangyan Chen, Huichao Yan, Wenchu Li, Jianrong Lin
Differentially expressed microRNAs were detected to explore the molecular mechanisms of diapause termination. The total small RNA of diapause-destined silkworm eggs and HCl-treated eggs was extracted and then sequenced using HiSeq high-throughput method. 44 novel miRNAs were discovered. Compared to those in the diapause-destined eggs, 61 miRNAs showed significant changes in the acid-treated eggs, with 23 being up-regulated and 38 being down-regulated. The potential target genes of differentially expressed miRNAs were predicted by miRanda...
2017: PloS One
https://www.readbyqxmd.com/read/28675377/investigation-of-novel-superparamagnetic-ni0-5zn0-5fe2o4-albumen-nanoparticles-for-controlled-delivery-of-anti-cancer-drug
#8
M Qasim, Khushnuma Asghar, Gangappa Dharmapuri, Dibakar Das
In the present work, multifunctional Ni0.5Zn0.5Fe2O4@albumen (NZF@Alb) and doxorubicin loaded Ni0.5Zn0.5Fe2O4@albumen (NZF@Alb-Dox) core-shell nanoparticles have been prepared by a green and simple method using inexpensive chicken egg albumen and have been characterized for different physiochemical properties. The structural, morphological, thermal and magnetic properties of the prepared nanoparticles have been investigated by XRD, HRTEM, FESEM, FTIR, TGA, and VSM techniques. Superparamagnetic Ni0.5Zn0.5Fe2O4 nanoparticles (NZF NPs) with the mean size ~20 nm was coated with albumen matrix by an ultrasonication process...
July 4, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28663368/betaine-is-accumulated-via-transient-choline-dehydrogenase-activation-during-mouse-oocyte-meiotic-maturation
#9
Taylor McClatchie, Megan Meredith, Mariame O Ouédraogo, Sandy Slow, Michael Lever, Mellissa R W Mann, Steven H Zeisel, Jacquetta M Trasler, Jay M Baltz
Betaine (N,N,N-trimethylglycine) plays key roles in mouse eggs and preimplantation embryos first in a novel mechanism of cell volume regulation and second as a major methyl donor in blastocysts, but its origin is unknown. Here, we determined that endogenous betaine was present at low levels in germinal vesicle (GV) stage mouse oocytes before ovulation, but reached high levels in the mature, ovulated egg. However, no betaine transport into oocytes was detected during meiotic maturation. Since betaine can be synthesized in mammalian cells via choline dehydrogenase (CHDH; EC1...
June 29, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28659876/improvement-strategies-cost-effective-production-and-potential-applications-of-fungal-glucose-oxidase-god-current-updates
#10
REVIEW
Manish K Dubey, Andleeb Zehra, Mohd Aamir, Mukesh Meena, Laxmi Ahirwal, Siddhartha Singh, Shruti Shukla, Ram S Upadhyay, Ruben Bueno-Mari, Vivek K Bajpai
Fungal glucose oxidase (GOD) is widely employed in the different sectors of food industries for use in baking products, dry egg powder, beverages, and gluconic acid production. GOD also has several other novel applications in chemical, pharmaceutical, textile, and other biotechnological industries. The electrochemical suitability of GOD catalyzed reactions has enabled its successful use in bioelectronic devices, particularly biofuel cells, and biosensors. Other crucial aspects of GOD such as improved feeding efficiency in response to GOD supplemental diet, roles in antimicrobial activities, and enhancing pathogen defense response, thereby providing induced resistance in plants have also been reported...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28571695/influenza-viruses-production-evaluation-of-a-novel-avian-cell-line-duckcelt%C3%A2-t17
#11
Emma Petiot, Anaïs Proust, Aurélien Traversier, Laurent Durous, Frédéric Dappozze, Marianne Gras, Chantal Guillard, Jean-Marc Balloul, Manuel Rosa-Calatrava
The influenza vaccine manufacturing industry is looking for production cell lines that are easily scalable, highly permissive to multiple viruses, and more effective in term of viral productivity. One critical characteristic of such cell lines is their ability to grow in suspension, in serum free conditions and at high cell densities. Influenza virus causing severe epidemics both in human and animals is an important threat to world healthcare. The repetitive apparition of influenza pandemic outbreaks in the last 20years explains that manufacturing sector is still looking for more effective production processes to replace/supplement embryonated egg-based process...
May 29, 2017: Vaccine
https://www.readbyqxmd.com/read/28544760/a-novel-pattern-of-yolk-processing-in-developing-snake-eggs-colubridae-lampropeltini-and-its-functional-and-evolutionary-implications
#12
Kathryn G Powers, Daniel G Blackburn
Early amniotic vertebrates evolved large-yolked eggs that permitted production of well-developed, terrestrial hatchlings. This reproductive pattern required new mechanisms for cellularizing the yolk and mobilizing it for embryonic use. In birds, cells that line the yolk sac cavity phagocytose and digest the yolk material, a pattern that is commonly assumed to be universal among oviparous amniotes. However, recent evidence challenges the assumption that all squamate reptiles conform to the avian developmental pattern...
May 24, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28528111/protection-of-%C3%AE-carotene-from-chemical-degradation-in-emulsion-based-delivery-systems-using-antioxidant-interfacial-complexes-catechin-egg-white-protein-conjugates
#13
Luping Gu, Yujie Su, Mengqi Zhang, Cuihua Chang, Junhua Li, David Julian McClements, Yanjun Yang
The aim of the present study was to fabricate catechin-egg white protein (CT-EWP) conjugates as novel food-grade antioxidant emulsifiers designed to improve the physicochemical stability of β-carotene (BC) emulsions. CT-EWP conjugates were synthesized using free radical grafting, and the formation of conjugates was confirmed by electrophoresis and liquid chromatography-mass spectrometry. The physicochemical stability of BC emulsions was characterized by measuring alterations in particle size, ζ-potential and BC retention...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28499203/efficient-protein-digestion-using-immobilized-trypsin-onto-tannin-modified-fe3o4-magnetic-nanoparticles
#14
Keziban Atacan, Bekir Çakıroğlu, Mahmut Özacar
Fe3O4 magnetic nanoparticles (MNPs) were prepared via solvothermal method. A commercially available trypsin was covalently immobilized onto MNPs modified with tannin (T) via a novel binding process. The morphology, structure, surface and magnetic properties of the obtained nanostructures were characterized comprehensively. The Fe3O4 MNPs had a saturation magnetization value of 60.18emu/g at room temperature, while the tannin modified Fe3O4 MNPs, and the trypsin immobilized on tannin modified-Fe3O4 MNPs possessed a saturation magnetization value of 57...
May 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28479482/embryonic-expression-of-festina-lente-fel-a-novel-maternal-gene-in-the-oligochaete-annelid-tubifex-tubifex
#15
Takuma Nakamura, Inori Shiomi, Takashi Shimizu
We have cloned and characterized the expression of a novel maternal gene festina lente (designated Ttu-fel) from the clitellate annelid Tubifex tubifex. Northern blot analyses have shown that Ttu-fel mRNA is approximately 8 kbp in length and that its expression is restricted to oocytes undergoing maturation division and early embryos up to 22-cell stage. Maternal transcripts of Ttu-fel are first detected in oocytes in the ovary of young adults (ca. 40 days after hatching); its expression continues in growing oocytes in the ovisac...
May 4, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28470499/coffee-its-roasted-form-and-their-residues-cause-birth-failure-and-shorten-lifespan-in-dengue-vectors
#16
Hamady Dieng, Salbiah Binti Ellias, Tomomitsu Satho, Abu Hassan Ahmad, Fatimah Abang, Idris Abd Ghani, Sabina Noor, Hamdan Ahmad, Wan Fatma Zuharah, Ronald E Morales Vargas, Noppawan P Morales, Cirilo N Hipolito, Siriluck Attrapadung, Gabriel Tonga Noweg
In dengue mosquitoes, successful embryonic development and long lifespan are key determinants for the persistence of both virus and vector. Therefore, targeting the egg stage and vector lifespan would be expected to have greater impacts than larvicides or adulticides, both strategies that have lost effectiveness due to the development of resistance. Therefore, there is now a pressing need to find novel chemical means of vector control. Coffee contains many chemicals, and its waste, which has become a growing environmental concern, is as rich in toxicants as the green coffee beans; these chemicals do not have a history of resistance in insects, but some are lost in the roasting process...
June 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28466047/a-sensitive-haemadsorption-technique-based-rt-pcr-for-concentration-and-detection-of-newcastle-disease-virus-from-clinical-samples-and-allantoic-fluid
#17
Perumal Arumugam Desingu, Shambhu Dayal Singh, Kuldeep Dhama, Obli Rajendran Vinodhkumar, Yashpal Singh Malik
The present study describes the exploitation of haemadsorption (HAd) property of the Newcastle disease virus (NDV) for the development of a novel sensitive HAd technique based RT-PCR for detection of NDV from clinical samples of virus infected experimental birds. The NDV propagated allantoic fluid from the infected embryonated chicken eggs or supernatant of the processed clinical samples (tissue triturate, cloaca and tracheal swabs) from the experimentally infected birds were added with chicken red blood cells (RBC) to adsorb the virus on RBC's surface...
September 2016: Virusdisease
https://www.readbyqxmd.com/read/28455449/identification-of-a-new-small-ubiquitin-like-modifier-sumo-interacting-motif-in-the-e3-ligase-piasy
#18
Kawaljit Kaur, Hyewon Park, Nootan Pandey, Yoshiaki Azuma, Roberto N De Guzman
Small ubiquitin-like modifier (SUMO) conjugation is a reversible post-translational modification process implicated in the regulation of gene transcription, DNA repair, and cell cycle. SUMOylation depends on the sequential activities of E1 activating, E2 conjugating, and E3 ligating enzymes. SUMO E3 ligases enhance transfer of SUMO from the charged E2 enzyme to the substrate. We have previously identified PIASy, a member of the Siz/protein inhibitor of activated STAT (PIAS) RING family of SUMO E3 ligases, as essential for mitotic chromosomal SUMOylation in frog egg extracts and demonstrated that it can mediate effective SUMOylation...
June 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28430907/food-intake-affects-sperm-egg-fusion-through-the-gip-psg17-axis-in-mice
#19
Tatsunori Shimizu, Takehiro Sato, Katsushi Tsukiyama, Hiroki Fujita, Shunsuke Kato, Manabu Hoizumi, Hiromitsu Shirasawa, Takuma Narita, Yukihiro Terada, Yutaka Seino, Yuichiro Yamada
In addition to overeating, starvation also reduces fecundity in mammals. However, little is known about the molecular mechanisms linking food intake to fertility, especially in males. Gastric inhibitory polypeptide (GIP), which is released from intestinal K-cells after meal ingestion, stimulates insulin secretion from pancreatic β-cells through the action of incretin and has several extra-pancreatic effects. Here, we identified GIP receptor (Gipr) expression in mouse spermatids. Microarray analysis revealed that pregnancy-specific glycoprotein 17 (Psg17), a potential CD9-binding partner, was significantly decreased in GIP receptor-knockout (Gipr-/-) testes...
April 19, 2017: Endocrinology
https://www.readbyqxmd.com/read/28422159/spastic-paraplegia-linked-phospholipase-papla1-is-necessary-for-development-reproduction-and-energy-metabolism-in-drosophila
#20
Martina Gáliková, Peter Klepsatel, Judith Münch, Ronald P Kühnlein
The human PAPLA1 phospholipase family is associated with hereditary spastic paraplegia (HSP), a neurodegenerative syndrome characterized by progressive spasticity and weakness of the lower limbs. Taking advantage of a new Drosophila PAPLA1 mutant, we describe here novel functions of this phospholipase family in fly development, reproduction, and energy metabolism. Loss of Drosophila PAPLA1 reduces egg hatchability, pre-adult viability, developmental speed, and impairs reproductive functions of both males and females...
April 19, 2017: Scientific Reports
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