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yolk enzyme treatment

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https://www.readbyqxmd.com/read/28817948/effects-of-dietary-supplementation-of-resveratrol-on-performance-egg-quality-yolk-cholesterol-and-antioxidant-enzyme-activity-of-laying-hens
#1
Z H Feng, J G Gong, G X Zhao, X Lin, Y C Liu, K W Ma
1. This experiment was conducted to evaluate the effects of dietary supplementation of resveratrol on laying performance, egg quality, egg yolk cholesterol and antioxidant enzyme activities of laying hens. 2. A total of 360 Beijing PINK-1 laying hens (60 weeks old) were randomly distributed among five dietary treatments, each of which included 6 replicates of 12 hens. Dietary treatments were basal diet supplemented with 0 (control), 0.5, 1.0, 2.0 and 4.0 g/kg diet resveratrol. The study lasted for 9 weeks including 1 week of adaptation and 8 weeks of the main experimental period...
September 4, 2017: British Poultry Science
https://www.readbyqxmd.com/read/28697990/avian-antibodies-igy-against-trypanosoma-cruzi-purification-and-characterization-studies
#2
Thirssa H Grando, Matheus D Baldissera, Mariângela F de Sá, Guilherme M do Carmo, Bianca Carolina Z Porto, Gisele S V Aguirre, Maria Isabel Azevedo, Francielli P K de Jesus, Janio M Santurio, Michele R Sagrillo, Lenita Moura Stefani, Silvia Gonzalez Monteiro
Trypanosoma cruzi is a flagellated protozoan belonging to the Trypanosomatidae family, the etiologic agent of Chagas disease. Currently, there is neither a licensed vaccine nor effective treatment, characterizing an unmet clinical need. The IgY refers to the egg yolk immunoglobulin (Y=yolk) and its production and use are subjects of many studies due to the diversity of its diagnostic and therapeutic applications. Several researchers have shown that the use of specific IgY may prevent and/or control infectious and parasitic diseases...
July 8, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28597141/apparent-metabolizable-energy-value-of-whole-date-palm-phoenix-dactylifera-l-and-its-possible-use-as-a-feedstuff-for-aged-laying-hens
#3
Mohamad Hamed Salajegheh, Mostafa Yousef Elahi, Mohamad Salarmoini, Akbar Yaghobfar
This study was conducted to evaluate the feeding value of whole date palm (WDP) with and without enzyme supplementation on aged laying hen's performance. Apparent metabolizable energy value of WDP was determined by the total collection method using ten adult leghorn cockerels. WDP was substituted with a corn-soybean meal basal diet at 40% level, and then the metabolizable energy of this experimental diet and basal diet was determined. After that, a total number of 256 Bovanz 95-week-old hens were randomly allocated into eight groups consisting of four replicates of eight birds each, based on a 4 × 2 factorial arrangement of the treatments...
August 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28416387/differential-regulation-of-the-lipoxygenase-pathway-in-shrimp-hepatopancreases-and-ovaries-during-ovarian-development-in-the-black-tiger-shrimp-penaeus-monodon
#4
Wananit Wimuttisuk, Punsa Tobwor, Pacharawan Deenarn, Chakapong Intaraudom, Thapanee Pruksatrakul, Sutichai Nithithanasilp, Somjai Wongtripop, Suwanchai Phomklad, Panomkorn Chaitongsakul, Vanicha Vichai
Dietary polyunsaturated fatty acids (PUFAs) are critical to the success of ovarian development in marine crustaceans, especially for domesticated species such as the black tiger shrimp Penaeus monodon. These fatty acids are stored in a midgut gland called the hepatopancreas and subsequently serve as an energy source or are incorporated in yolk during ovarian development. PUFAs are known precursors of hydroxy fatty acids, including hydroxyeicosatetraenoic acid and hydroxyeicosapentaenoic acid (HEPE), which are catalyzed by lipoxygenases (LOX)...
May 27, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28410665/impact-of-dietary-precursor-ala-versus-preformed-dha-on-fatty-acid-profiles-of-eggs-liver-and-adipose-tissue-and-expression-of-genes-associated-with-hepatic-lipid-metabolism-in-laying-hens
#5
M Neijat, P Eck, J D House
Dietary omega-3 polyunsaturated fatty acids (n-3 PUFA), including alpha-linolenic acid (ALA) and preformed longer chain PUFA (LCPUFA, particularly docosahexaenoic acid, DHA) differ in their egg LCPUFA enrichment efficiency. However, mechanisms leading to these differences are unclear. To this end, omega-3 PUFA contents in different lipid classes, including triacylglycerol (TAG) and total phospholipid (PL) in yolk, liver and adipose, as well as the expression of key hepatic enzymes in lipid metabolism were evaluated in laying hens in response to changes in dietary supply...
April 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28391155/an-approach-to-clarify-the-effect-mechanism-of-glyphosate-on-body-malformations-during-embryonic-development-of-zebrafish-daino-rerio
#6
Ekrem Sulukan, Mine Köktürk, Hamid Ceylan, Şükrü Beydemir, Mesut Işik, Muhammed Atamanalp, Saltuk Buğrahan Ceyhun
In this study, it has been investigated that the effects of glyphosate, which is a herbicide within organophosphate and unselective widely used in agriculture on enzyme activity of carbonic anhydrase, production of reactive oxygen species, cell apoptosis and body morphology during the embryonic development of zebrafish. To this end, it has been treated embryo with 1, 5, 10 and 100 mg/L gyphosate at 96 h. The embryos treated with glyphosate from 4 hpf were evaluated by considering the survival rates, hatching rates, body malformations under the stereo microscope in 24, 48, 72 and 96th hours...
August 2017: Chemosphere
https://www.readbyqxmd.com/read/28203647/the-potential-of-blue-lupins-as-a-protein-source-in-the-diets-of-laying-hens
#7
Michael R F Lee, Sarah Parkinson, Hannah R Fleming, Vince J Theobald, Dave K Leemans, Tony Burgess
Layers diets typically contain 15-20% soya due to its high crude protein content (ca. 36%). Reliance on soya for protein can result in large increases in cost of feed due to the law of supply and demand as a global commodity. Lupin grains have high protein content (35-40%) but previous experience with white lupins has shown toxic effects in poultry due to high levels alkaloids and poor performance due to anti-nutritional Non-starch polysaccharides (NSP). Here blue lupins either processed or whole were trialled for their potential as a protein source...
December 2016: Veterinary and Animal Science
https://www.readbyqxmd.com/read/28149163/dietary-inclusion-of-raw-faba-bean-instead-of-soybean-meal-and-enzyme-supplementation-in-laying-hens-effect-on-performance-and-egg-quality
#8
M E Abd El-Hack, M Alagawany, V Laudadio, R Demauro, V Tufarelli
An experiment was conducted with 160 Hisex Brown laying hens to evaluate the effect of different inclusion levels of faba bean (FB) and enzyme supplementation on productive performance and egg quality parameters. The experimental diets consisted of five levels of FB: 0% (control), 25%, 50%, 75% and 100%, substituting soybean meal (SBM), and two levels of enzyme supplementation (0 or 250 mg/kg). Each dietary treatment was assigned to four replicate groups and the experiment lasted 22 weeks. A positive relationship (P < 0...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28111290/suppression-of-neu1-sialidase-delays-the-absorption-of-yolk-sac-in-medaka-oryzias-latipes-accompanied-with-the-accumulation-of-%C3%AE-2-3-sialo-glycoproteins
#9
Sena Ryuzono, Ryo Takase, Yuko Kamada, Takanori Ikenaga, Petros Kingstone Chigwechokha, Masaharu Komatsu, Kazuhiro Shiozaki
Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiological functions in fish are still unclear. Here, to understand Neu1 significance in medaka embryogenesis, sialidase translation knockdown was carried out with one-cell stage fertilized egg using morpholino oligo injection...
April 2017: Biochimie
https://www.readbyqxmd.com/read/27896806/nitric-oxide-synthase-during-early-embryonic-development-in-silkworm-bombyx-mori-gene-expression-enzyme-activity-and-tissue-distribution
#10
Ryo Kitta, Marina Kuwamoto, Yumi Yamahama, Keisuke Mase, Hiroshi Sawada
To elucidate the mechanism for embryonic diapause or the breakdown of diapause in Bombyx mori, we biochemically analyzed nitric oxide synthase (NOS) during the embryogenesis of B. mori. The gene expression and enzyme activity of B. mori NOS (BmNOS) were examined in diapause, non-diapause, and HCl-treated diapause eggs. In the case of HCl-treated diapause eggs, the gene expression and enzyme activity of BmNOS were induced by HCl treatment. However, in the case of diapause and non-diapause eggs during embryogenesis, changes in the BmNOS activity and gene expressions did not coincide except 48-60 h after oviposition in diapause eggs...
December 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27540477/recent-advances-in-pathogenesis-assessment-and-treatment-of-atherosclerosis
#11
REVIEW
J David Spence
In recent years, there have been a number of advances in the pathogenesis and treatment of atherosclerosis and in assessing prognosis in carotid atherosclerosis. Risk stratification to improve vascular prevention by identifying patients most likely to benefit from intensive therapy is much improved by measuring carotid plaque burden. In patients with asymptomatic carotid stenosis, a number of modalities can be used to identify the 10-15% who could benefit from endarterectomy or stenting. Transcranial Doppler embolus detection, echolucency and ulceration on 3D ultrasound, intraplaque hemorrhage on magnetic resonance imaging (MRI), and reduced cerebrovascular reserve are useful already; new approaches including plaque texture on ultrasound and imaging of plaque inflammation and early calcification on positron emission tomography/computed tomography (PET/CT) are in development...
2016: F1000Research
https://www.readbyqxmd.com/read/27143774/diet-supplementation-with-a-specific-melon-concentrate-improves-oviduct-antioxidant-defenses-and-egg-characteristics-in-laying-hens
#12
J Carillon, F Barbé, S Barial, M Saby, A Sacy, J-M Rouanet
The objectives of this study were to investigate the effects of a specific melon concentrate on oviduct antioxidant defenses and egg characteristics of laying hens.Lohmann Brown hens were assigned to 2 treatment groups (n = 16 in each). One group was supplemented with the melon concentrate (26 mg/kg of feed) during 6 wk. The other group was composed of untreated hens, which served as control. Eggs were collected, weighed (yolk, albumen, shell), and analyzed (Haugh unit and albumen pH relevant for egg freshness) at the end of the supplementation period...
August 1, 2016: Poultry Science
https://www.readbyqxmd.com/read/27022687/primary-endodermal-epithelial-cell-culture-from-the-yolk-sac-membrane-of-japanese-quail-embryos
#13
Han Jen Lin, Siou Huei Wang, Yu Hui Pan, Shih-Torng Ding
We established an endodermal epithelial cell culture model (EEC) for studying the function of certain enzymes and proteins in mediating nutrient utilization by avian embryos during development. Fertilized Japanese quail eggs were incubated at 37 °C for 5 days and then yolk sac membranes (YSM) were collected to establish the EEC culture system. We isolated the embryonic endoderm layer from YSM, and sliced the membrane into 2 - 3 mm pieces and partially digested with collagenase before seeding in 24-well culture plates...
March 10, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/26980114/lipovitellin-as-an-antigen-to-improve-the-precision-of-sandwich-elisa-for-quantifying-zebrafish-danio-rerio-vitellogenin
#14
Jun Wang, Xiaona Zhang, Ruihou Shan, Shuwei Ma, Hua Tian, Wei Wang, Shaoguo Ru
Vitellogenin (Vtg) in zebrafish (Danio rerio) is a core biomarker for screening environmental estrogens in test guidelines of the Organization for Economic Cooperation and Development. To accurately quantify zebrafish Vtg, lipovitellin (Lv), the main Vtg-derived yolk protein, was used as the antigen to establish a sandwich enzyme-linked immunosorbent assay (ELISA). The purified Lv was a phospholipoglycoprotein with apparent molecular weight of ~445kDa, and separated into three polypeptides corresponding to ~117, ~102, and ~23...
July 2016: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/26847677/effects-of-cerium-oxide-supplementation-to-laying-hen-diets-on-performance-egg-quality-some-antioxidant-enzymes-in-serum-and-lipid-oxidation-in-egg-yolk
#15
RANDOMIZED CONTROLLED TRIAL
S C Bölükbaşı, A A Al-Sagan, H Ürüşan, M K Erhan, O Durmuş, N Kurt
This study was conducted to determine the effects of dietary cerium oxide levels (0, 100, 200, 300 or 400 mg/kg) on the laying performance, egg quality, some blood serum parameters and egg lipid peroxidation of laying hen. In total, one hundred and twenty 22-week-old brown Lohman LSL laying hens were randomly assigned to five groups equally (n = 24). Each treatment was replicated six times. Dietary supplementation of cerium oxide had no significant effect on feed intake and egg weight. The addition of cerium oxide to the laying hens' feed improved feed conversion ratio and increased (p < 0...
August 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/26615573/effect-of-oral-antiseptic-agents-on-phospholipase-and-proteinase-enzymes-of-candida-albicans
#16
Banu Uygun-Can, Tanju Kadir, Birsay Gumru
OBJECTIVE: Candida-associated denture stomatitis is the most prevalent form of oral candida infections among the denture wearers. Generally, antiseptic oral rinses used in the treatment of these infections are considered as an adjunct or alternative antifungal treatment. Studies have suggested that the intraoral concentrations of antiseptics decrease substantially to the sub-therapeutic levels on account of the dynamics of the oral cavity. This condition yields the question about the minimum antiseptic concentration that effect the character or pathogenesis of Candida during treatment...
February 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/26475102/antibodies-against-venom-of-the-snake-deinagkistrodon-acutus
#17
Chi-Hsin Lee, Yu-Ching Lee, Meng-Huei Liang, Sy-Jye Leu, Liang-Tzung Lin, Jen-Ron Chiang, Yi-Yuan Yang
Snake venom protein from Deinagkistrodon acutus (DA protein), one of the major venomous species in Taiwan, causes hemorrhagic symptoms that can lead to death. Although horse-derived antivenin is a major treatment, relatively strong and detrimental side effects are seen occasionally. In our study, yolk immunoglobulin (IgY) was purified from eggs, and DA protein was recognized using Western blotting and an enzyme-linked immunosorbent assay (ELISA), similar to therapeutic horse antivenin. The ELISA also indicated that specific IgY antibodies were elicited after the fifth booster, plateaued, and lasted for at least 3 months...
October 16, 2015: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/26456929/toxicity-and-cytochrome-p450-1a-mrna-induction-by-6-formylindolo-3-2-b-carbazole-ficz-in-chicken-and-japanese-quail-embryos
#18
Maria E Jönsson, Anna Mattsson, Siraz Shaik, Björn Brunström
The tryptophan derivative formylindolo[3,2-b]carbazole (FICZ) binds with high ligand affinity to the aryl hydrocarbon receptor (AHR) and is readily degraded by AHR-regulated cytochrome P450 family 1 (CYP1) enzymes. Whether in vivo exposure to FICZ can result in toxic effects has not been examined and the main objective of this study was to determine if FICZ is embryotoxic in birds. We examined toxicity and CYP1 mRNA induction of FICZ in embryos from chicken (Gallus domesticus) and Japanese quail (Coturnix japonica) exposed to FICZ (2-200μgkg(-1)) by yolk and air sac injections...
January 2016: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/26173627/effect-of-flaxseed-on-the-fatty-acid-profile-of-egg-yolk-and-antioxidant-status-of-their-neonatal-offspring-in-huoyan-geese
#19
W Chen, Y Y Jiang, J P Wang, B X Yan, Y Q Huang, Z X Wang
The aim of this study was to evaluate the effects of geese's maternal diet supplemented with flaxseed on the fatty acid profiles of egg yolks and the antioxidant status of their offspring. A total of 288 female Huoyan geese (42 weeks old) were randomly allotted to four experimental groups in this 56-day experiment and fed on diets containing flaxseed at 0% (control), 5%, 10% and 15%, respectively. There were nine replicate pens per treatment, with eight geese per replicate pen. The concentration of α-linolenic acid (linear, P<0...
November 2015: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/25825784/oral-peptide-specific-egg-antibody-to-intestinal-sodium-dependent-phosphate-co-transporter-2b-is-effective-at-altering-phosphate-transport-in-vitro-and-in-vivo
#20
Elizabeth A Bobeck, Erica M Hellestad, Jordan M Sand, Michelle L Piccione, Jeff W Bishop, Christian Helvig, Martin Petkovich, Mark E Cook
Hyperimmunized hens are an effective means of generating large quantities of antigen specific egg antibodies that have use as oral supplements. In this study, we attempted to create a peptide specific antibody that produced outcomes similar to those of the human pharmaceutical, sevelamer HCl, used in the treatment of hyperphosphatemia (a sequela of chronic renal disease). Egg antibodies were generated against 8 different human intestinal sodium-dependent phosphate cotransporter 2b (NaPi2b) peptides, and hNaPi2b peptide egg antibodies were screened for their ability to inhibit phosphate transport in human intestinal Caco-2 cell line...
June 2015: Poultry Science
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