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egg enzyme treatment

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https://www.readbyqxmd.com/read/28321408/novel-sequential-screening-and-enhanced-production-of-fibrinolytic-enzyme-by-bacillus-sp-ind12-using-response-surface-methodology-in-solid-state-fermentation
#1
Ponnuswamy Vijayaraghavan, P Rajendran, Samuel Gnana Prakash Vincent, Arumugaperumal Arun, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Oh Young Kwon, Young Ock Kim
Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings, Bacillus sp. IND12 was selected for fibrinolytic enzyme production. Bacillus sp. IND12 effectively used cow dung for its growth and enzyme production (687 ± 6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28283013/-molecular-characterization-of-echinococcus-granulosus-isolates-obtained-from-different-hosts
#2
Emrah Erdoğan, Bora Özkan, Fatih Mutlu, Serkan Karaca, İzzet Şahin
Echinococcus granulosus is a parasite that can be seen throughout the world. So far, five species of genus Echinococcus have been identified as parasite in people: E.granulosus, E.multilocularis, E.vogeli, E.oligarthrus, E.shiquicus. Larval (metacestod) form of parasite settles in internal organs of hoofed animals (cattle, goats, pigs, horses, sheep, etc.) and human; the adult form is found in small intestine of final host, canine. Disease caused by parasite called as "Cystic echinococcosis" (CE) is an important health problem and causes economic losses in many countries including our country that livestock is common...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28203647/the-potential-of-blue-lupins-as-a-protein-source-in-the-diets-of-laying-hens
#3
Michael R F Lee, Sarah Parkinson, Hannah R Fleming, Vince J Theobald, Dave K Leemans, Tony Burgess
Layers diets typically contain 15-20% soya due to its high crude protein content (ca. 36%). Reliance on soya for protein can result in large increases in cost of feed due to the law of supply and demand as a global commodity. Lupin grains have high protein content (35-40%) but previous experience with white lupins has shown toxic effects in poultry due to high levels alkaloids and poor performance due to anti-nutritional Non-starch polysaccharides (NSP). Here blue lupins either processed or whole were trialled for their potential as a protein source...
December 2016: Veterinary and Animal Science
https://www.readbyqxmd.com/read/28149163/dietary-inclusion-of-raw-faba-bean-instead-of-soybean-meal-and-enzyme-supplementation-in-laying-hens-effect-on-performance-and-egg-quality
#4
M E Abd El-Hack, M Alagawany, V Laudadio, R Demauro, V Tufarelli
An experiment was conducted with 160 Hisex Brown laying hens to evaluate the effect of different inclusion levels of faba bean (FB) and enzyme supplementation on productive performance and egg quality parameters. The experimental diets consisted of five levels of FB: 0% (control), 25%, 50%, 75% and 100%, substituting soybean meal (SBM), and two levels of enzyme supplementation (0 or 250 mg/kg). Each dietary treatment was assigned to four replicate groups and the experiment lasted 22 weeks. A positive relationship (P < 0...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28144765/control-of-haemonchus-contortus-in-sheep-using-basidiocarps-of-agaricus-blazei-murril
#5
Thallyta Maria Vieira, Leydiana Duarte Fonseca, Gabriela Almeida Bastos, Viviane de Oliveira Vasconcelos, Maria Luíza França Silva, Franciellen Morais-Costa, Adriano Vinícius de Paiva Ferreira, Neide Judith Faria de Oliveira, Eduardo Robson Duarte
OBJECTIVE: This study evaluated the effects in vitro and in vivo of Agaricus blazei against Haemonchus contortus in sheep. METHODS: The in vitro efficacy of aqueous extract on egg hatching inhibition (EHI) was investigated and after 72 h incubation with varying concentrations the effects on, blastomeres, embryonated eggs, and first stage larvae (L1) were evaluated. Larval development inhibition (LDI) for dry powder and the aqueous extract were evaluated in fecal cultures of sheep infected with H...
January 31, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28111290/suppression-of-neu1-sialidase-delays-the-absorption-of-yolk-sac-in-medaka-oryzias-latipes-accompanied-with-the-accumulation-of-%C3%AE-2-3-sialo-glycoproteins
#6
Sena Ryuzono, Ryo Takase, Yuko Kamada, Takanori Ikenaga, Petros Kingstone Chigwechokha, Masaharu Komatsu, Kazuhiro Shiozaki
Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiological functions in fish are still unclear. Here, to understand Neu1 significance in medaka embryogenesis, sialidase translation knockdown was carried out with one-cell stage fertilized egg using morpholino oligo injection...
April 2017: Biochimie
https://www.readbyqxmd.com/read/28107750/effect-of-clinically-approved-hdac-inhibitors-on-plasmodium-leishmania-and-schistosoma-parasite-growth
#7
Ming Jang Chua, Megan S J Arnold, Weijun Xu, Julien Lancelot, Suzanne Lamotte, Gerald F Späth, Eric Prina, Raymond J Pierce, David P Fairlie, Tina S Skinner-Adams, Katherine T Andrews
Malaria, schistosomiasis and leishmaniases are among the most prevalent tropical parasitic diseases and each requires new innovative treatments. Targeting essential parasite pathways, such as those that regulate gene expression and cell cycle progression, is a key strategy for discovering new drug leads. In this study, four clinically approved anti-cancer drugs (Vorinostat, Belinostat, Panobinostat and Romidepsin) that target histone/lysine deacetylase enzymes were examined for in vitro activity against Plasmodium knowlesi, Schistosoma mansoni, Leishmania amazonensis and L...
December 23, 2016: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/28043567/detection-of-mycobacterium-avium-subsp-paratuberculosis-in-cattle-by-using-indirect-absorbed-elisa-enzyme-linked-immunosorbent-assay-system-and-culture-in-alborz-province-iran
#8
Hedyeh Teymouri, Nader Mosavari, Hadi Poor Taghi
OBJECTIVE/BACKGROUND: Mycobacterium avium subsp. paratuberculosis is a slow growing, Gram-positive, and acid-fast bacillus. It is the causative agent of the chronic enteritis disease of ruminants called Johne's disease. Most of the infected animals are young. However, most clinical cases belong to adult cattle aged 3-5years. Due to a prolonged incubation period, identification of subclinically infected animals is one of the most crucial problems in Johne's disease. The aim of this study was to detect M...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28027870/novel-triclabendazole-prodrug-a-highly-water-soluble-alternative-for-the-treatment-of-fasciolosis
#9
Miguel Flores-Ramos, Froylán Ibarra-Velarde, Helgi Jung-Cook, Alicia Hernández-Campos, Yolanda Vera-Montenegro, Rafael Castillo
In this work we present the synthesis, aqueous solubility and stability, hydrolysis by alkaline phosphatase, and in vivo fasciolicidal activity in sheep of a highly water soluble phosphate salt prodrug of triclabendazole (MFR-5). The aqueous solubility of MFR-5 at pH 7 was 88,000-fold that of triclabendazole. MFR-5 showed excellent aqueous stability (>95% after 26h) at pH 7, making it ideal for developing pharmaceutical compositions in the form of solutions that can easily be hydrolyzed by the enzyme alkaline phosphatase (t=13...
December 2, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27941611/transcriptional-response-of-silkworm-bombyx-mori-eggs-to-o%C3%A2-or-hcl-treatment
#10
Jing Gong, Sha Tian, Xia Zhou, Huan Yang, Yong Zhu, Yong Hou
Diapause is a common biological phenomenon that occurs in many organisms, including fish, insects, and nematodes. In the silkworm (Bombyx mori), diapause generally occurs in the egg stage. Treatment with O₂, HCl, or other compounds can prevent egg diapause. Here, we characterized the transcriptomic responses of newly laid eggs treated with O₂ or HCl. Digital gene expression analysis showed that 610 genes in O₂-treated eggs and 656 in HCl-treated eggs were differentially expressed. Of these, 343 genes were differentially expressed in both treatments...
December 7, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27916895/validation-of-folate-enriched-eggs-as-a-functional-food-for-improving-folate-intake-in-consumers
#11
Leslie Altic, Helene McNulty, Leane Hoey, Liadhan McAnena, Kristina Pentieva
Functional foods enriched with folate may be beneficial as a means of optimizing folate status in consumers. We recently developed novel eggs enriched with folate through folic acid supplementation of the hen's feed, but their potential to influence consumer folate status is unknown because the natural folate forms incorporated into the eggs may not necessarily be retained during storage and cooking. This study aimed to determine the stability of natural folates in folate-enriched eggs under typical conditions of storage and cooking...
November 30, 2016: Nutrients
https://www.readbyqxmd.com/read/27896806/nitric-oxide-synthase-during-early-embryonic-development-in-silkworm-bombyx-mori-gene-expression-enzyme-activity-and-tissue-distribution
#12
Ryo Kitta, Marina Kuwamoto, Yumi Yamahama, Keisuke Mase, Hiroshi Sawada
To elucidate the mechanism for embryonic diapause or the breakdown of diapause in Bombyx mori, we biochemically analyzed nitric oxide synthase (NOS) during the embryogenesis of B. mori. The gene expression and enzyme activity of B. mori NOS (BmNOS) were examined in diapause, non-diapause, and HCl-treated diapause eggs. In the case of HCl-treated diapause eggs, the gene expression and enzyme activity of BmNOS were induced by HCl treatment. However, in the case of diapause and non-diapause eggs during embryogenesis, changes in the BmNOS activity and gene expressions did not coincide except 48-60 h after oviposition in diapause eggs...
December 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27869499/capsaicinoids-improve-egg-production-by-regulating-ovary-nuclear-transcription-factors-against-heat-stress-in-quail
#13
N Sahin, C Orhan, M Tuzcu, V Juturu, K Sahin
1. To examine the molecular mechanism of capsaicinoid supplementation from capsicum extract, laying Japanese quail (n = 180, 5 weeks old) were reared either at 22°C for 24 h/d (thermoneutral, TN) or at 34°C for 8 h/d (heat stress, HS) and fed on one of three diets containing 0, 25 or 50 mg of capsaicinoids per kilogram for 12 weeks (2 × 3 factorial arrangement). 2. The results revealed that exposure to HS decreased feed consumption by 10.7% and egg production by 13.6%, increased serum and ovary malondialdehyde (MDA) levels by 66...
April 2017: British Poultry Science
https://www.readbyqxmd.com/read/27865412/expression-and-localization-of-matrix-metalloproteinases-mmp-2-7-9-and-their-tissue-inhibitors-timp-2-3-in-the-chicken-oviduct-during-pause-in-laying-induced-by-tamoxifen
#14
Agnieszka Leśniak-Walentyn, Anna Hrabia
Induced pause in egg laying simulates natural molting events in which the hen's reproductive organs regress and rejuvenate. Such processes require extracellular matrix remodeling that is maintained, at least in part, by the action of proteolytic enzymes known as matrix metalloproteinases (MMPs). Nevertheless, information concerning the expression and hormonal regulation of MMP system members in chickens is scarce. Therefore, MMP-2, -7, and -9 and their tissue inhibitors (TIMP-2, -3) expression and localization were investigated in all segments of the domestic hen oviduct (infundibulum, magnum, isthmus, shell gland, vagina) during a pause in egg laying induced by tamoxifen (TMX)-an estrogen receptor modulator...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27821159/ceratonia-siliqua-pod-extract-ameliorates-schistosoma-mansoni-induced-liver-fibrosis-and-oxidative-stress
#15
Ebtesam M Al-Olayan, Manal F El-Khadragy, Reem A Alajmi, Mohamed S Othman, Amira A Bauomy, Shaimaa R Ibrahim, Ahmed E Abdel Moneim
BACKGROUND: Schistosomiasis is a prevalent parasitic disease found predominantly in tropical and sub-tropical areas of the developing world, with the second highest socioeconomic and public health burden despite strenuous control efforts. In the present study, we aimed to investigate the ameliorative effects of Ceratonia siliqua pod extract (CPE) on liver fibrosis and oxidative stress in mice infected with Schistosoma mansoni. METHODS: The schistosomal hepatopathologic mouse model was established by tail immersion with schistosomal cercaria...
November 8, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27810261/effect-of-paecilomyces-lilacinus-trichoderma-harzianum-and-trichoderma-virens-fungal-extracts-on-the-hatchability-of-ancylostoma-eggs
#16
Bianca Delgado Menezes Hofstätter, Anelise Oliveira da Silva Fonseca, Fernando de Souza Maia Filho, Julia de Souza Silveira, Beatriz Maroneze Persici, Luciana Pötter, Andressa Silveira, Zaida Inês Antoniolli, Daniela Isabel Brayer Pereira
BACKGROUND: Ancylostoma species have demanded attention due to their zoonotic potential. The use of anthelmintics is the usual method to prevent environmental contamination by Ancylostoma eggs and larvae. Nematophagous fungi have been widely used in their biological control due to the fungus ability to capture and digest free nematode forms. AIMS: The aim of this study was to evaluate the effect of four different fungal extracts of Paecilomyces lilacinus (n=2), Trichoderma harzianum (n=1) and Trichoderma virens (n=1) isolates on the hatchability of Ancylostoma eggs...
October 31, 2016: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/27798285/endocannabinoid-system-and-pregnancy
#17
REVIEW
Fernando Correa, Manuel L Wolfson, Paula Valchi, Julieta Aisemberg, Ana María Franchi
The endocannabinoid system (eCS), is a complex system, comprising the main endogenous ligands anandamide and 2-arachidonoyl glycerol, the cannabinoid receptors CB1 and CB2 and the biosynthetic and degrading enzymes. Cumulative evidence shows that the eCS plays an important role in reproduction, from egg fertilization to parturition. Therefore, alterations in this system, either by recreation/therapeutic use of cannabis or deregulation of the endogenous cannabinoids, might lead to adverse pregnancy outcomes, including retardation in embryo development, poor blastocyst implantation, inhibition of decidualization, miscarriage and compromised placentation...
December 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27761283/efficiency-of-direct-microscopy-of-stool-samples-using-an-antigen-specific-adhesin-test-for-entamoeba-histolytica
#18
Arzu İrvem, Kamil Özdil, Zuhal Çalışkan, Muhterem Yücel
BACKGROUND: E. histolytica is among the common causes of acute gastroenteritis. The pathogenic species E. histolytica and the nonpathogenic species E. dispar cannot be morphologically differentiated, although correct identification of these protozoans is important for treatment and public health. In many laboratories, the screening of leukocytes, erythrocytes, amoebic cysts, trophozoites and parasite eggs is performed using Native-Lugol's iodine for pre-diagnosis. AIMS: In this study, we aimed to investigate the frequency of E...
September 2016: Balkan Medical Journal
https://www.readbyqxmd.com/read/27744603/single-and-combined-impacts-of-vitamin-a-and-selenium-in-diet-on-productive-performance-egg-quality-and-some-blood-parameters-of-laying-hens-during-hot-season
#19
Mohamed E Abd El-Hack, Khalid Mahrose, Ali A Askar, Mahmoud Alagawany, Muhammad Arif, Muhammad Saeed, Farzana Abbasi, Rab Nawaz Soomro, Farman Ali Siyal, Maria Tabassum Chaudhry
A study was conducted using 162 Bovans laying hens to investigate the impacts of extra dietary vitamin A (0, 8000, 16,000 IU/kg), selenium (0, 0.25, 0.50 mg/kg), and their combinations on the performance, egg quality, and blood biological parameters of laying hens during summer months. Supplemental vitamin A up to 16,000 IU/kg diet significantly (P < 0.05) improved all productive traits studied except feed intake which increased with 8000 IU/kg diet compared with control. Feed intake and feed conversion of hens fed diet supplemented with selenium revealed high statistical (P = 0...
October 15, 2016: Biological Trace Element Research
https://www.readbyqxmd.com/read/27664643/changes-in-the-antigenicity-and-allergenicity-of-ovalbumin-in-chicken-egg-white-by-n-acetylglucosaminidase
#20
Ho-Young Park, Taek Joon Yoon, Ha Hyung Kim, Young Shin Han, Hee-Don Choi
Ovalbumin (OVA), an (hen) egg allergen, is one of the most abundant glycoprotein allergens associated with IgE-mediated hypersensitivity through the T-helper type 2 immune response. The effect of deglycosylation of the N-terminal glycan in OVA on allergenicity and antigenicity after N-acetylglucosaminidase treatment was studied. N-acetylglucosaminidase-treated OVA (N-OVA) evaluated using an enzyme-linked immunosorbent assay, respectively. N-OVA significantly (p<0.05) OVA-specific IgE and histamine levels...
February 15, 2017: Food Chemistry
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