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egg enzyme treatment

Arzu İrvem, Kamil Özdil, Zuhal Çalışkan, Muhterem Yücel
BACKGROUND: E. histolytica is among the common causes of acute gastroenteritis. The pathogenic species E. histolytica and the nonpathogenic species E. dispar cannot be morphologically differentiated, although correct identification of these protozoans is important for treatment and public health. In many laboratories, the screening of leukocytes, erythrocytes, amoebic cysts, trophozoites and parasite eggs is performed using Native-Lugol's iodine for pre-diagnosis. AIMS: In this study, we aimed to investigate the frequency of E...
September 2016: Balkan Medical Journal
Mohamed E Abd El-Hack, Khalid Mahrose, Ali A Askar, Mahmoud Alagawany, Muhammad Arif, Muhammad Saeed, Farzana Abbasi, Rab Nawaz Soomro, Farman Ali Siyal, Maria Tabassum Chaudhry
A study was conducted using 162 Bovans laying hens to investigate the impacts of extra dietary vitamin A (0, 8000, 16,000 IU/kg), selenium (0, 0.25, 0.50 mg/kg), and their combinations on the performance, egg quality, and blood biological parameters of laying hens during summer months. Supplemental vitamin A up to 16,000 IU/kg diet significantly (P < 0.05) improved all productive traits studied except feed intake which increased with 8000 IU/kg diet compared with control. Feed intake and feed conversion of hens fed diet supplemented with selenium revealed high statistical (P = 0...
October 15, 2016: Biological Trace Element Research
Ho-Young Park, Taek Joon Yoon, Ha Hyung Kim, Young Shin Han, Hee-Don Choi
Ovalbumin (OVA), an (hen) egg allergen, is one of the most abundant glycoprotein allergens associated with IgE-mediated hypersensitivity through the T-helper type 2 immune response. The effect of deglycosylation of the N-terminal glycan in OVA on allergenicity and antigenicity after N-acetylglucosaminidase treatment was studied. N-acetylglucosaminidase-treated OVA (N-OVA) evaluated using an enzyme-linked immunosorbent assay, respectively. N-OVA significantly (p<0.05) OVA-specific IgE and histamine levels...
February 15, 2017: Food Chemistry
Fangjie Cao, Lizhen Zhu, Hui Li, Song Yu, Chengju Wang, Lihong Qiu
In the past few decades, extensive application of azoxystrobin has led to great concern regarding its adverse effects on aquatic organisms. The objective of the present study was to evaluate the reproductive toxicity of azoxystrobin to zebrafish. After adult zebrafish of both sexes were exposed to 2, 20 and 200 μg/L azoxystrobin for 21 days, egg production, the fertilization rate, the gonadosomatic index (GSI) and hepatosomatic index (HSI), 17β-estradiol (E2), testosterone (T) and vitellogenin (Vtg) concentrations, and histological alterations in the gonads and livers were measured...
September 8, 2016: Environmental Pollution
Seockmo Ku, Eduardo Ximenes, Thomas Kreke, Kirk Foster, Amanda J Deering, Michael R Ladisch
We report detection of less than 13 CFU of Salmonella per 25 g egg white within 7 hours by concentrating the bacteria using microfiltration through 0.2 μm cutoff polyethersulfone hollow fiber membranes. A combination of enzyme treatment, controlled cross-flow on both sides of the hollow fibers, and media selection were key to controlling membrane fouling so that rapid concentration and the subsequent detection of low numbers of microbial cells were achieved. We leveraged the protective effect of egg white proteins and peptone so that the proteolytic enzymes did not attack the living cells while hydrolyzing the egg white proteins responsible for fouling...
August 24, 2016: Biotechnology Progress
J David Spence
In recent years, there have been a number of advances in the pathogenesis and treatment of atherosclerosis and in assessing prognosis in carotid atherosclerosis. Risk stratification to improve vascular prevention by identifying patients most likely to benefit from intensive therapy is much improved by measuring carotid plaque burden. In patients with asymptomatic carotid stenosis, a number of modalities can be used to identify the 10-15% who could benefit from endarterectomy or stenting. Transcranial Doppler embolus detection, echolucency and ulceration on 3D ultrasound, intraplaque hemorrhage on magnetic resonance imaging (MRI), and reduced cerebrovascular reserve are useful already; new approaches including plaque texture on ultrasound and imaging of plaque inflammation and early calcification on positron emission tomography/computed tomography (PET/CT) are in development...
2016: F1000Research
Nai-Xin Kang, Yuan-Jian Zhu, Jian-Ping Zhao, Wei-Feng Zhu, Yan-Li Liu, Qiong-Ming Xu, Hong-Xiang Zhuge, Ikhlas A Khan, Shi-Lin Yang
The present study was undertaken to investigate whether hederacochiside C (HSC) possesses antischistosomal effects and anti-inflammatory response activities in Schistosoma japonicum-infected mice. Different concentrations of HSC were administrated to the mice infected by schistosomula or adult worm by intravenous injection twice a day for five consecutive days. The total worm burden, female worm burden, and the egg burden in liver of mice treated with 400 mg/kg HSC were fewer than those in non-treated ones...
July 25, 2016: Journal of Asian Natural Products Research
Edward-Sam Edwin, Prabhakaran Vasantha-Srinivasan, Sengottayan Senthil-Nathan, Annamalai Thanigaivel, Athirstam Ponsankar, Venkatraman Pradeepa, Selvaraj Selin-Rani, Kandaswamy Kalaivani, Wayne B Hunter, Ahmed Abdel-Megeed, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi
The current study investigated the toxic effect of the leaf extract compound andrographolide from Andrographis paniculata (Burm.f) against the dengue vector Ae. aegypti. GC-MS analysis revealed that andrographolide was recognized as the major chemical constituent with the prominent peak area compared with other compounds. All isolated toxic compounds were purified and confirmed through RP-HPLC against chemical standards. The larvicidal assays established at 25ppm of bioactive compound against the treated instars of Ae...
November 2016: Acta Tropica
Xiaoping Wu, Shulei Pan, Wuduo Zhou, Yijiang Wu, Yifan Huang, Baocheng Wu
In July 2009, some farms of breeding Muscovy ducks on the peak of egg laying suffered the decrease of hatching rate and the quality of the eggs showing low mortality and no evident respiratory symptoms. The swelling and congestive ovary was visible after autopsy. This study was brought out for the diagnosis of these cases. The virus was isolated and identified by the methods of virus culture in chicken embryo, physical and chemical properties test, hemagglutinin test, NDV (Newcastle diseases Virus) interference test, electron microscope observation, pathogenicity test and the gene sequence analysis...
March 2016: Bing du Xue Bao, Chinese Journal of Virology
Kaveh Taghipoor, Saeed Keyvanshokooh, Amir Parviz Salati, Hossein Pasha-Zanoosi, Samad Bahrami Babaheydari
The objective of the present study was to examine the antioxidant status of rainbow trout (Oncorhynchus mykiss) during the early stages of development (fertilized egg, eyed egg, alevin and fry) as an effect of triploidy induction. Eggs and milt were taken from eight females and six males. After insemination, the eggs were incubated at 10°C for 10min. Half of the fertilized eggs were then subjected to heat-shock for 10min submerged in a 28°C water bath to induce triploidy. The remainder were incubated normally and used as diploid controls...
August 2016: Animal Reproduction Science
Zhenhua Yan, Guanghua Lu, Qiuxia Ye, Jianchao Liu
A partial life-cycle study with zebrafish (Danio rerio) was conducted to evaluate the long-term effects of antibiotics, norfloxacin (NOR) and sulfamethoxazole (SMX). A series of bio-endpoints correlated to the growth, development, and reproduction was assessed. The results showed that the body weight and the condition factor were depressed by SMX at 200 μg/L during the growth period. Meanwhile, the activities of metabolic enzyme (ethoxyresorufin O-deethylase, EROD) and antioxidant enzymes (superoxide dismutase, SOD and catalase, CAT) were stimulated in all cases...
September 2016: Environmental Science and Pollution Research International
Chang-Chun Li, Guo-Yuan Li, Yue-Li Yun, Jian Chen, Zeng-Tao Zhang, Yu Peng
The objective of the present study was to assess the ecotoxicological responses of Pardosa pseudoannulata to a common environmental pollutant, cadmium. Third-instar spiderlings and adult spiders were exposed to sublethal concentrations of CdCl2 solution in their drinking water. The Cd content in P. pseudoannulata adults increased significantly with the number of days of exposure to a 0.2 mM CdCl2 solution, when exposed to 2 mM CdCl2 solution, the Cd content in the spiders increased sharply in the first two (male) or three (female) weeks, and then no significant changes were recorded following with the next three (male) or two (female) weeks exposure...
July 2016: Bulletin of Environmental Contamination and Toxicology
Hye Seong Hwang, Heajin Park, Jihye Kim, Jai Yeon Choi, Young Kwang Lee, Ho-Young Park, Hee-Don Choi, Ha Hyung Kim
Ovalbumin (OA) is the most abundant ingredient of chicken egg-white allergenic proteins. In the present study we investigated the possibility of reducing OA allergenicity by treatment with a natural protein exhibiting N-acetylglucosaminidase (NA) activity. Ascidian is cultivated as a food resource in northeast Asia. The ascidian viscera NA (AVNA) with almost no other exoglycosidases or proteolytic enzymes was isolated by applying size-exclusion chromatography to a protein precipitate of ascidian viscera. Intact OA was mixed with AVNA containing 0...
June 17, 2016: Biochemical and Biophysical Research Communications
K Yogesh
Thermal processing ensures safety and enhances the shelf-life of most of the food products. It alters the structural-chemical composition, modifies heat labile components, as well as affects the functional properties of food products. This has driven the development of non-thermal food processing techniques, primarily for extending the shelf-life of different food products. These techniques are currently also being evaluated for their effects on product processing, quality and other safety parameters. Pulsed electric field (PEF) is an example of non-thermal technique which can be applied for a variety of purpose in the food processing industry...
February 2016: Journal of Food Science and Technology
J Carillon, F Barbé, S Barial, M Saby, A Sacy, J-M Rouanet
The objectives of this study were to investigate the effects of a specific melon concentrate on oviduct antioxidant defenses and egg characteristics of laying hens.Lohmann Brown hens were assigned to 2 treatment groups (n = 16 in each). One group was supplemented with the melon concentrate (26 mg/kg of feed) during 6 wk. The other group was composed of untreated hens, which served as control. Eggs were collected, weighed (yolk, albumen, shell), and analyzed (Haugh unit and albumen pH relevant for egg freshness) at the end of the supplementation period...
August 1, 2016: Poultry Science
Inayat U Rahman, Mohammad Bashir, Muhammad Idrees, Khalil Ur Rahman, Muhammad Iqbal Khan
OBJECTIVE: To determine the effect of Zubex (aqueous extract of Curcuma longa linn, Iron murakab, Eugenia jambolana, Lin seed or flflax seed, processed egg shell calcium and Asphaltum) on plasma sialic acid (PSA) along with cardiovascular (CV) risk factors in streptozotocin-induced diabetic rats. METHODS: Thirty male albino rats were divided into groups (1-6) with 5 rats in each group. Group 1 and 2 served as normal control (NC) and diabetic control (DC), respectively, and were given normal saline only...
April 27, 2016: Chinese Journal of Integrative Medicine
Alessio Soggiu, Cristian Piras, Stefano Levi Mortera, Isabella Alloggio, Andrea Urbani, Luigi Bonizzi, Paola Roncada
UNLABELLED: Grana Padano is a typical Italian Protected Designation of Origin (PDO) hard cheese largely consumed all over the world. The major problem during its production is represented by late blowing. Clostridia are gasogen bacteria responsible of the swelling during ripening, and they are partially counteracted by the use of egg white lysozyme as additive. In this work was applied, for the first time in cheese, a metaproteomic approach that identified the functional dynamics of microbial consortia in relation to the number of clostridial spores and lysozyme treatment using experimental samples of Grana Padano cheese...
September 16, 2016: Journal of Proteomics
Han Jen Lin, Siou Huei Wang, Yu Hui Pan, Shih-Torng Ding
We established an endodermal epithelial cell culture model (EEC) for studying the function of certain enzymes and proteins in mediating nutrient utilization by avian embryos during development. Fertilized Japanese quail eggs were incubated at 37 °C for 5 days and then yolk sac membranes (YSM) were collected to establish the EEC culture system. We isolated the embryonic endoderm layer from YSM, and sliced the membrane into 2 - 3 mm pieces and partially digested with collagenase before seeding in 24-well culture plates...
2016: Journal of Visualized Experiments: JoVE
Tino Heimburg, Alokta Chakrabarti, Julien Lancelot, Martin Marek, Jelena Melesina, Alexander-Thomas Hauser, Tajith B Shaik, Sylvie Duclaud, Dina Robaa, Frank Erdmann, Matthias Schmidt, Christophe Romier, Raymond J Pierce, Manfred Jung, Wolfgang Sippl
Schistosomiasis is a major neglected parasitic disease that affects more than 265 million people worldwide and for which the control strategy consists of mass treatment with the only available drug, praziquantel. In this study, a series of new benzohydroxamates were prepared as potent inhibitors of Schistosoma mansoni histone deacetylase 8 (smHDAC8). Crystallographic analysis provided insights into the inhibition mode of smHDAC8 activity by these 3-amidobenzohydroxamates. The newly designed inhibitors were evaluated in screens for enzyme inhibitory activity against schistosome and human HDACs...
March 24, 2016: Journal of Medicinal Chemistry
D Kumar, K Budachetri, V C Meyers, S Karim
As obligate blood-sucking ectoparasites, to avoid tissue damage, ticks must neutralize the reactive oxygen species (ROS) generated from uptake and digestion of a bloodmeal. Consequently, ticks utilize a battery of antioxidant molecules, including catalase (CAT), an enzyme that converts hydrogen peroxide (H2 O2 ) into water and oxygen. Here, we investigated the tick antioxidant machinery by exogenous injection of sublethal doses of H2 O2 or paraquat. The relative transcript levels of selected Amblyomma maculatum antioxidant targets in tissues were determined by quantitative reverse transcriptase PCR following treatment...
June 2016: Insect Molecular Biology
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