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egg enzyme treatment

Nanping Xiao, Fenghua Liu, Guangxi Zhou, Mingming Sun, Fengfu Ai, Zhanju Liu
Objective Dietary antigens are common luminal antigens in the gastrointestinal tract and have been considered to contribute to the pathogenesis of inflammatory bowel disease (IBD). We analyzed the levels of food-specific IgGs against a variety of dietary antigens, explored the clinical relevance of food allergy to the pathogenesis of IBD, and investigated whether or not infliximab (IFX) treatment could regulate the immune responses induced by dietary antigens. Methods A total of 301 IBD patients, including 201 patients with Crohn's disease (CD) and 100 patients with ulcerative colitis (UC), were recruited, and their serum food-specific IgGs against 14 food antigens were detected by a semi-quantitative enzyme linked immunosorbent assay (ELISA)...
May 18, 2018: Internal Medicine
Lisa-Marie Schaden, Miriam Wimmer-Teubenbacher, Johannes Poms, Peter Laggner, Karl Lohner, Stephan Sacher, Johannes G Khinast, Sharareh Salar-Behzadi
PURPOSE: The effect of different irradiation doses on the structure and activity of lyophilized powders of Hen Egg-White Lysozyme (HEWL) and alcohol dehydrogenase (ADH) was investigated using these substances as models for robust and sensitive proteins, respectively. Three doses were selected to cover the ranges of radio-sterilization (25kGy), treatment of blood products (25Gy) and annual background radiation dose (approximately 2mGy). The results offer an initial screening of different irradiation doses and support the development of X-ray imaging methods as non-destructive process analytical technology (PAT) tools for detecting the visible particulate matters in such products...
May 7, 2018: Pharmaceutical Research
Hongmei Wang, Ruili Zhang, Yangyang Song, Tianqi Li, Ming Ge
Studies have shown that cadmium can cause chicken testicular damage, but a protective effect of Ganoderma triterpenoids on cadmium-induced testicular damage in chickens has not yet been reported. The present study was designed to research the protective effect of Ganoderma triterpenoids on cadmium-induced testicular damage in chicken. Eighty healthy 7-day-old Hyline egg laying chickens were randomly divided into four groups with 20 in each group. The control group was fed with normal full-fodder, the model group was fed with normal full-fodder with 140 mg/kg of CdCl2 , the Ganoderma triterpenoid treatment group was fed with a full-fodder diet containing 140 mg/kg of CdCl2 and 0...
May 2, 2018: Biological Trace Element Research
S A El-Refai, A F Atia, S F Mahmoud
Schistosomiasis is a chronic debilitating parasitic disease that causes hepatic damage and is known to be endemic in developing countries. Recent control strategies for schistosomiasis depend exclusively on chemotherapeutic agents, specifically praziquantel. Unfortunately, praziquantel has low efficacy in the early phase of infection, and resistance to treatment is increasingly reported. The aim of this work was to find an alternative treatment by assessing the in vivo activity of aqueous extract of Callistemon citrinus against Schistosoma mansoni in both prepatent and patent phases in experimentally infected mice...
May 2, 2018: Journal of Helminthology
J Herrera, B Saldaña, L Cámara, J D Berrocoso, G G Mateos
The influence of grinding size of the main cereal of the diet on production and egg quality traits was studied in brown hens from 33 to 65 wk of age. The experiment was completely randomized with 6 treatments arranged as a 3 × 2 factorial with 3 main cereals (barley, corn, and wheat) and 2 grinding size of the cereal (6 and 10 mm screen). Each treatment was replicated 11 times (10 hens/replicate). Diets were isonutritive and contained 2,740 kcal/kg AMEn and 16.8% CP. Egg production, ADFI, egg weight, and feed conversion ratio (FCR) were determined by period (4 wk) and for the entire experiment...
April 17, 2018: Poultry Science
Silvana Dinaintang Harikedua, Christina A Mireles DeWitt
Brine injection of natural proteases, dried egg white (EW), and dried potato extract (PE), was investigated as a means to prevent softening during cooking of Pacific whiting fillets. Treatments included fillets injected with Water (W), 3% sodium chloride and 3% sodium tripolyphosphate brine (B), B with 0.1% xanthan gum (BXG ), B with 1%, 2%, or 3% EW (B1-3EW ), B with 1%, 2%, or 3% PE (B1-3PE ) or non-injected (NI). Non-injected Pacific cod (Cod) was also utilized as a reference for texture analysis. Fillets were subsequently cooked using cooking protocol 1 (90 °C for 20 min) or cooking protocol 2 (60 °C for 30 min then 90 °C for 20 min)...
April 16, 2018: Journal of Food Science
E V N Beshay, A A Rady, A F Afifi, A H Mohamed
Despite the seriousness of schistosomiasis, its treatment depends only on praziquantel (PZQ), which has begun to lose its efficacy against the emergent Schistosoma mansoni-resistant strains. Therefore, the discovery of a novel schistosomicidal drug is an urgent priority. This study was designed to evaluate treatment with Cucurbita pepo L. (pumpkin) seed oil (PSO) alone and combined with PZQ against S. mansoni in experimentally infected mice. The study involved five groups: GI was the normal control; GII was the infected control; GIII was treated with an oral dose of PZQ of 500 mg/kg/day for two successive days, starting in the sixth week post infection; GIV was treated with an oral dose of PSO of 50 mg/kg/day for four weeks, starting in the fourth week post infection; and GV was treated with combined PSO-PZQ...
April 15, 2018: Journal of Helminthology
Grace VanHoy, Michelle Carman, Greg Habing, Jeffrey Lakritz, C Austin Hinds, Andrew Niehaus, Ray M Kaplan, Antoinette E Marsh
Haemonchosis in camelids remains a challenging disease to treat, and prevention has become increasingly problematic due to widespread anthelmintic resistance. Barbervax® is an adjuvanted vaccine containing natural H-11, H-gal-GP antigens obtained from Haemonchus contortus adults via a proprietary process and solubilized in Quil A. This vaccine is approved for use in Australia, after demonstrating its safety and efficacy in sheep and goats. There are no published studies evaluating Barbervax in other ruminants/pseudoruminants such as camelids which can be parasitized with H...
March 15, 2018: Veterinary Parasitology
Fernando Schemelzer Moraes Bezerra, Joames Kauffimann Freitas Leal, Mariana Silva Sousa, Marta Cristhiany Cunha Pinheiro, Alberto Novaes Ramos, Vanessa Silva-Moraes, Naftale Katz
Schistosomiasis is still a public health problem in Brazil. The Kato-Katz test is the most frequently used diagnostic method for Schistosoma mansoni infection. However, it lacks sensitivity in areas of low prevalence. We have assessed the positivity rate of S. mansoni infection in Bananeiras, a village on Capistrano, Ceara, Brazil by performing a point-of-care test in urine to determine the circulating cathodic antigens (POC-CCA), and we compared the findings with those of the Kato-Katz technique for egg detection in stool and an enzyme-linked immunosorbent assay for specific antibodies against adult worms (SWAP-ELISA) in serum before treatment (baseline)...
June 2018: Acta Tropica
Paul Ogongo, Thomas M Kariuki, R Alan Wilson
The inadequacy of current diagnostics for the detection of low worm burdens in humans means that schistosomiasis mansoni is more widespread than previously acknowledged. With the inception of mass drug treatment programmes aimed at disease elimination and the advent of human vaccine trials, the need for more sensitive diagnostics is evident. In this review, we evaluate the merits and limitations of the principal diagnostic methods, namely detection of eggs in faeces; anti-schistosome antibodies in serum; parasite-derived proteins and glycans in serum or urine; parasite DNA in blood, faeces or urine...
March 6, 2018: Parasitology
Sutisa Khempaka, Prapot Maliwan, Supattra Okrathok, Wittawat Molee
Two experiments were conducted to investigate the potential use of dried cassava pulp (DCP) supplemented with enzymes as an alternative feed ingredient in laying hen diets. In experiment 1, 45 laying hens (Isa Brown) aged 45 weeks were placed in individual cages to measure nutrient digestibility for 10 days. Nine dietary treatments were control and DCP as a replacement for corn at 20, 25, 30, and 35% supplemented with mixed enzymes (cellulase, glucanase, and xylanase) at 0.10 and 0.15%. Results showed that the use of DCP at 20-35% added with mixed enzymes had no negative effects on dry matter digestibility, while organic matter digestibility and nitrogen retention decreased with increased DCP up to 30-35% in diets...
February 24, 2018: Tropical Animal Health and Production
You Wu, Jun Ding, Bing Xu, Linlin You, Linquan Ge, Guoqing Yang, Fang Liu, David Stanley, Qisheng Song, Jincai Wu
Aside from their intended actions, fungicides can drive pest insect outbreaks due to virtually continuous use and pest evolution. Small brown planthopper (SBPH), Laodelphax striatellus, outbreaks occurred recently in many provinces in China, with devastating rice losses. Because exposure to the fungicide jinggangmycin (JGM) increased reproduction of the brown plant hopper, Nilaparvata lugens, via its influence on fatty acid synthase, we posed the hypothesis that JGM and carbendazim (CBM) influence SBPH reproduction via their influence on enzymes involved in other aspects of lipid metabolism...
March 2, 2018: Journal of Proteome Research
Jitse M van der Meer
The genetic regulation of anterior-posterior segment pattern development has been elucidated in detail for Drosophila, but it is not canonical for insects. A surprising diversity of regulatory mechanisms is being uncovered not only between insect orders, but also within the order of the Diptera. The question is whether the same diversity of regulatory mechanisms exists within other insect orders. I show that anterior puncture of the egg of the pea beetle Callosobruchus maculatus submerged in RNase can induce double abdomen development suggesting a role for maternal mRNA...
January 2018: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
G K Walker, S Jalukar, J Brake
Hatching eggs collected from resident broiler breeders at 48 wk of age were used to produce male and female chicks that were assigned sex separately to 96 new litter pens and fed either a 0 or 50 g/MT RFC (refined functional carbohydrate feed additive derived from yeast) diet. There were 24 replicate pens of 12 broilers each per diet per sex. Feed intake and BW were determined at 14, 28, and 42 d of age. Litter was sampled by pen using sterile socks at 35 d and tested for Salmonella spp. using an enzyme linked fluorescence assay method...
April 1, 2018: Poultry Science
Keiichi Yoshida, Woojin Kang, Akihiro Nakamura, Natsuko Kawano, Maito Hanai, Mami Miyado, Yoshitaka Miyamoto, Maki Iwai, Toshio Hamatani, Hidekazu Saito, Kenji Miyado, Akihiro Umezawa
The ubiquitin-proteasome system, which is initiated by a single ubiquitin-activating enzyme E1 (UBE1), is involved in male reproduction via spermatogenesis and function in mammals. Here we explored the influence of UBE1-specific inhibitor, 4[4-(5-nitro-furan-2-ylmethylene)-3,5-dioxo-pyrazolidin-1-yl]-benzoic acid ethyl ester (pyrazone-41 or PYR-41) in female reproduction. UBE-1 was detected by immunoblotting and immunocytochemistry in mouse eggs and was localized mainly under the egg plasma membrane. PYR-41 pretreatment suppresses the development of eggs into two-cell embryos...
March 2018: Reproductive Toxicology
E D N S Abeyrathne, X Huang, D U Ahn
Egg white contains many functionally important proteins: ovalbumin (54%), ovotransferrin (12%), ovomucoid (11%), ovoglobulin (G2 and G3, 8%), ovomucin (3.5%), and lysozyme (3.5%) are major proteins, while ovoinhibitors, ovomacroglobulin, ovoglycoprotein, ovoflavoprotein, thiamine-binding proteins, and avidin are minor proteins present in egg white. These proteins, as well as the peptides derived from the proteins, have been recognized for their functional importance as antioxidant, antimicrobial, metal-chelating, anti-viral, anti-tumour, and angiotensin-converting enzyme (ACE)-inhibitory activities...
April 1, 2018: Poultry Science
Myoungjin Son, Catherine B Chan, Jianping Wu
SCOPE: The renin-angiotensin system (RAS) is a major contributor to the development of insulin resistance and its related complications. Egg white ovotransferrin-derived tripeptides, IRW (Ile-Arg-Trp), IQW (Ile-Gln-Trp), or LKP (Leu-Lys-Pro) are previously identified as the inhibitors of angiotensin-converting enzyme (ACE), a key enzyme in the RAS. This study aims at determining whether these peptides are effective in improving insulin resistance, and their mechanisms of action, in a rat derived skeletal muscle cell line (L6 cells)...
February 2018: Molecular Nutrition & Food Research
D Ruan, A M Fouad, Q L Fan, W Chen, W G Xia, S Wang, Y Y Cui, Y Wang, L Yang, C T Zheng
The study investigated the effects of increasing content of corn distillers' dried grains with solubles (DDGS) in the diets of laying ducks on oxidative status, laying performance, egg quality, and egg yolk fatty acid composition. Longyan females (1080) with similar BW at 17 wk of age were randomly assigned to 6 treatment groups, each consisting of 6 replicates of 30 birds. The basal diet (I) was a typical corn-soybean ration while the experimental diets (II to VI) substituted corn DDGS for soybean meal and wheat bran and a small reduction of corn...
February 1, 2018: Poultry Science
Nicoleta Stănciuc, Alexandra Andreea Creţu, Iuliana Banu, Iuliana Aprodu
BACKGROUND: Ovomucoid (OVM) is the dominant allergen found in egg white. The heat-induced changes on chicken OVM structure and antigenic properties were assessed at acidic, neutral and alkaline pH values. RESULTS: The fluorescence spectroscopy measurements indicated changes in the conformation of OVM caused by both pH and thermal treatment. The OVM molecule exhibited higher exposure of hydrophobic residues at 7.0, as indicated by the synchronous spectra, intrinsic fluorescence and quenching experiments...
December 6, 2017: Journal of the Science of Food and Agriculture
Guillermina Canesini, Cora Stoker, Germán H Galoppo, Milena L Durando, María V Tschopp, Enrique H Luque, Mónica M Muñoz-de-Toro, Jorge G Ramos
Caiman latirostris is a species with temperature dependent sex determination (TSD), which implies that the incubation temperature of the eggs is the main factor that determines the sex during a thermo-sensitive period (TSP). However, estrogens play a critical role in this process. The administration of 17β-estradiol (E2 ) previous to TSP overrides the effects of male incubation temperature, producing phenotypic females. This effect has been defined as sex reversal or estrogen-induced sex determination (E2 SD)...
April 1, 2018: General and Comparative Endocrinology
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