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https://www.readbyqxmd.com/read/28443982/enzyme-linked-immunosorbent-assay-for-diagnosis-of-amphimerus-spp-liver-fluke-infection-in-humans
#1
William Cevallos, Manuel Calvopiña, Victoria Nipáz, Belén Vicente-Santiago, Julio López-Albán, Pedro Fernández-Soto, Ángel Guevara, Antonio Muro
BACKGROUND: Amphimerus spp. is a liver fluke that infects humans and domestic animals. It is highly prevalent in some Ecuadorian communities. Currently, diagnosis is based on the microscopic observation of eggs in faeces, but this has variable sensitivity. More sensitive methods are needed for diagnostic testing. OBJECTIVE: The main objective of this work was to develop an enzyme-linked immunosorbent assay (ELISA) using crude antigens from Amphimerus spp. adult worms to detect anti-Amphimerus IgG in human sera...
May 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28431176/effect-of-melatonin-on-renewal-of-chicken-small-intestinal-mucosa
#2
J Li, R X Li, G Liu, C F Lv, Y L Mi, C Q Zhang
A higher concentration of melatonin (MEL) in the intestine - even more than that in the plasma and pineal gland - implies its putative important role in the gastrointestinal structural or functional regulation. However, little evidence has shown that MEL can regulate the physiological functions of the intestinal mucosa. In this study, fertilized chicken eggs were treated with MEL (0.1 to 10 μg/d) from embryonic d 12 (E12) to post-hatching d 6 (D6), and the small intestine samples were collected at D6 to determine the changes in mucosal construction and function...
April 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/28428873/a-maize-landrace-that-emits-defense-volatiles-in-response-to%C3%A2-herbivore-eggs-possesses-a-strongly-inducible-terpene-synthase-gene
#3
Amanuel Tamiru, Toby J A Bruce, Annett Richter, Christine M Woodcock, Charles A O Midega, Jörg Degenhardt, Segenet Kelemu, John A Pickett, Zeyaur R Khan
Maize (Zea mays) emits volatile terpenes in response to insect feeding and egg deposition to defend itself against harmful pests. However, maize cultivars differ strongly in their ability to produce the defense signal. To further understand the agroecological role and underlying genetic mechanisms for variation in terpene emission among maize cultivars, we studied the production of an important signaling component (E)-caryophyllene in a South American maize landrace Braz1006 possessing stemborer Chilo partellus egg inducible defense trait, in comparison with the European maize line Delprim and North American inbred line B73...
April 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28410665/impact-of-dietary-precursor-ala-versus-preformed-dha-on-fatty-acid-profiles-of-eggs-liver-and-adipose-tissue-and-expression-of-genes-associated-with-hepatic-lipid-metabolism-in-laying-hens
#4
M Neijat, P Eck, J D House
Dietary omega-3 polyunsaturated fatty acids (n-3 PUFA), including alpha-linolenic acid (ALA) and preformed longer chain PUFA (LCPUFA, particularly docosahexaenoic acid, DHA) differ in their egg LCPUFA enrichment efficiency. However, mechanisms leading to these differences are unclear. To this end, omega-3 PUFA contents in different lipid classes, including triacylglycerol (TAG) and total phospholipid (PL) in yolk, liver and adipose, as well as the expression of key hepatic enzymes in lipid metabolism were evaluated in laying hens in response to changes in dietary supply...
April 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28373144/efficacy-of-soluble-glycoprotein-fraction-from-allium-sativum-purified-by-size-exclusion-chromatography-on-murine-schistosomiasis-mansoni
#5
Ibrahim Aly, Eman E Taher, Hoda El-Sayed, Faten A Mohammed, Gehan ELnain, Rabab S Hamad, Elsayed M Bayoumy
In this work, the efficiency of crude MeOH extracts and soluble glycoprotein fraction of Allium sativum purified by size-exclusion chromatography (SEC) on parasitological, histopathological and some biochemical parameters in Schistosoma mansoni infected mice were investigated. Animals were infected by tail immersion with 100 cercariae/each mouse and divided into five groups in addition to the normal control. The results revealed a significant decrease in mean worm burden in all treated mice especially in the group treated with soluble glycoprotein fraction of A...
March 31, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28321408/novel-sequential-screening-and-enhanced-production-of-fibrinolytic-enzyme-by-bacillus-sp-ind12-using-response-surface-methodology-in-solid-state-fermentation
#6
Ponnuswamy Vijayaraghavan, P Rajendran, Samuel Gnana Prakash Vincent, Arumugaperumal Arun, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Oh Young Kwon, Young Ock Kim
Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings, Bacillus sp. IND12 was selected for fibrinolytic enzyme production. Bacillus sp. IND12 effectively used cow dung for its growth and enzyme production (687 ± 6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28283013/-molecular-characterization-of-echinococcus-granulosus-isolates-obtained-from-different-hosts
#7
Emrah Erdoğan, Bora Özkan, Fatih Mutlu, Serkan Karaca, İzzet Şahin
Echinococcus granulosus is a parasite that can be seen throughout the world. So far, five species of genus Echinococcus have been identified as parasite in people: E.granulosus, E.multilocularis, E.vogeli, E.oligarthrus, E.shiquicus. Larval (metacestod) form of parasite settles in internal organs of hoofed animals (cattle, goats, pigs, horses, sheep, etc.) and human; the adult form is found in small intestine of final host, canine. Disease caused by parasite called as "Cystic echinococcosis" (CE) is an important health problem and causes economic losses in many countries including our country that livestock is common...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28203647/the-potential-of-blue-lupins-as-a-protein-source-in-the-diets-of-laying-hens
#8
Michael R F Lee, Sarah Parkinson, Hannah R Fleming, Vince J Theobald, Dave K Leemans, Tony Burgess
Layers diets typically contain 15-20% soya due to its high crude protein content (ca. 36%). Reliance on soya for protein can result in large increases in cost of feed due to the law of supply and demand as a global commodity. Lupin grains have high protein content (35-40%) but previous experience with white lupins has shown toxic effects in poultry due to high levels alkaloids and poor performance due to anti-nutritional Non-starch polysaccharides (NSP). Here blue lupins either processed or whole were trialled for their potential as a protein source...
December 2016: Veterinary and Animal Science
https://www.readbyqxmd.com/read/28149163/dietary-inclusion-of-raw-faba-bean-instead-of-soybean-meal-and-enzyme-supplementation-in-laying-hens-effect-on-performance-and-egg-quality
#9
M E Abd El-Hack, M Alagawany, V Laudadio, R Demauro, V Tufarelli
An experiment was conducted with 160 Hisex Brown laying hens to evaluate the effect of different inclusion levels of faba bean (FB) and enzyme supplementation on productive performance and egg quality parameters. The experimental diets consisted of five levels of FB: 0% (control), 25%, 50%, 75% and 100%, substituting soybean meal (SBM), and two levels of enzyme supplementation (0 or 250 mg/kg). Each dietary treatment was assigned to four replicate groups and the experiment lasted 22 weeks. A positive relationship (P < 0...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28144765/control-of-haemonchus-contortus-in-sheep-using-basidiocarps-of-agaricus-blazei-murril
#10
Thallyta Maria Vieira, Leydiana Duarte Fonseca, Gabriela Almeida Bastos, Viviane de Oliveira Vasconcelos, Maria Luíza França Silva, Franciellen Morais-Costa, Adriano Vinícius de Paiva Ferreira, Neide Judith Faria de Oliveira, Eduardo Robson Duarte
OBJECTIVE: This study evaluated the effects in vitro and in vivo of Agaricus blazei against Haemonchus contortus in sheep. METHODS: The in vitro efficacy of aqueous extract on egg hatching inhibition (EHI) was investigated and after 72 h incubation with varying concentrations the effects on, blastomeres, embryonated eggs, and first stage larvae (L1) were evaluated. Larval development inhibition (LDI) for dry powder and the aqueous extract were evaluated in fecal cultures of sheep infected with H...
January 31, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28111290/suppression-of-neu1-sialidase-delays-the-absorption-of-yolk-sac-in-medaka-oryzias-latipes-accompanied-with-the-accumulation-of-%C3%AE-2-3-sialo-glycoproteins
#11
Sena Ryuzono, Ryo Takase, Yuko Kamada, Takanori Ikenaga, Petros Kingstone Chigwechokha, Masaharu Komatsu, Kazuhiro Shiozaki
Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiological functions in fish are still unclear. Here, to understand Neu1 significance in medaka embryogenesis, sialidase translation knockdown was carried out with one-cell stage fertilized egg using morpholino oligo injection...
April 2017: Biochimie
https://www.readbyqxmd.com/read/28107750/effect-of-clinically-approved-hdac-inhibitors-on-plasmodium-leishmania-and-schistosoma-parasite-growth
#12
Ming Jang Chua, Megan S J Arnold, Weijun Xu, Julien Lancelot, Suzanne Lamotte, Gerald F Späth, Eric Prina, Raymond J Pierce, David P Fairlie, Tina S Skinner-Adams, Katherine T Andrews
Malaria, schistosomiasis and leishmaniases are among the most prevalent tropical parasitic diseases and each requires new innovative treatments. Targeting essential parasite pathways, such as those that regulate gene expression and cell cycle progression, is a key strategy for discovering new drug leads. In this study, four clinically approved anti-cancer drugs (Vorinostat, Belinostat, Panobinostat and Romidepsin) that target histone/lysine deacetylase enzymes were examined for in vitro activity against Plasmodium knowlesi, Schistosoma mansoni, Leishmania amazonensis and L...
April 2017: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/28043567/detection-of-mycobacterium-avium-subsp-paratuberculosis-in-cattle-by-using-indirect-absorbed-elisa-enzyme-linked-immunosorbent-assay-system-and-culture-in-alborz-province-iran
#13
Hedyeh Teymouri, Nader Mosavari, Hadi Poor Taghi
OBJECTIVE/BACKGROUND: Mycobacterium avium subsp. paratuberculosis is a slow growing, Gram-positive, and acid-fast bacillus. It is the causative agent of the chronic enteritis disease of ruminants called Johne's disease. Most of the infected animals are young. However, most clinical cases belong to adult cattle aged 3-5years. Due to a prolonged incubation period, identification of subclinically infected animals is one of the most crucial problems in Johne's disease. The aim of this study was to detect M...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28027870/novel-triclabendazole-prodrug-a-highly-water-soluble-alternative-for-the-treatment-of-fasciolosis
#14
Miguel Flores-Ramos, Froylán Ibarra-Velarde, Helgi Jung-Cook, Alicia Hernández-Campos, Yolanda Vera-Montenegro, Rafael Castillo
In this work we present the synthesis, aqueous solubility and stability, hydrolysis by alkaline phosphatase, and in vivo fasciolicidal activity in sheep of a highly water soluble phosphate salt prodrug of triclabendazole (MFR-5). The aqueous solubility of MFR-5 at pH 7 was 88,000-fold that of triclabendazole. MFR-5 showed excellent aqueous stability (>95% after 26h) at pH 7, making it ideal for developing pharmaceutical compositions in the form of solutions that can easily be hydrolyzed by the enzyme alkaline phosphatase (t=13...
December 2, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27941611/transcriptional-response-of-silkworm-bombyx-mori-eggs-to-o%C3%A2-or-hcl-treatment
#15
Jing Gong, Sha Tian, Xia Zhou, Huan Yang, Yong Zhu, Yong Hou
Diapause is a common biological phenomenon that occurs in many organisms, including fish, insects, and nematodes. In the silkworm (Bombyx mori), diapause generally occurs in the egg stage. Treatment with O₂, HCl, or other compounds can prevent egg diapause. Here, we characterized the transcriptomic responses of newly laid eggs treated with O₂ or HCl. Digital gene expression analysis showed that 610 genes in O₂-treated eggs and 656 in HCl-treated eggs were differentially expressed. Of these, 343 genes were differentially expressed in both treatments...
December 7, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27916895/validation-of-folate-enriched-eggs-as-a-functional-food-for-improving-folate-intake-in-consumers
#16
Leslie Altic, Helene McNulty, Leane Hoey, Liadhan McAnena, Kristina Pentieva
Functional foods enriched with folate may be beneficial as a means of optimizing folate status in consumers. We recently developed novel eggs enriched with folate through folic acid supplementation of the hen's feed, but their potential to influence consumer folate status is unknown because the natural folate forms incorporated into the eggs may not necessarily be retained during storage and cooking. This study aimed to determine the stability of natural folates in folate-enriched eggs under typical conditions of storage and cooking...
November 30, 2016: Nutrients
https://www.readbyqxmd.com/read/27896806/nitric-oxide-synthase-during-early-embryonic-development-in-silkworm-bombyx-mori-gene-expression-enzyme-activity-and-tissue-distribution
#17
Ryo Kitta, Marina Kuwamoto, Yumi Yamahama, Keisuke Mase, Hiroshi Sawada
To elucidate the mechanism for embryonic diapause or the breakdown of diapause in Bombyx mori, we biochemically analyzed nitric oxide synthase (NOS) during the embryogenesis of B. mori. The gene expression and enzyme activity of B. mori NOS (BmNOS) were examined in diapause, non-diapause, and HCl-treated diapause eggs. In the case of HCl-treated diapause eggs, the gene expression and enzyme activity of BmNOS were induced by HCl treatment. However, in the case of diapause and non-diapause eggs during embryogenesis, changes in the BmNOS activity and gene expressions did not coincide except 48-60 h after oviposition in diapause eggs...
December 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27869499/capsaicinoids-improve-egg-production-by-regulating-ovary-nuclear-transcription-factors-against-heat-stress-in-quail
#18
N Sahin, C Orhan, M Tuzcu, V Juturu, K Sahin
1. To examine the molecular mechanism of capsaicinoid supplementation from capsicum extract, laying Japanese quail (n = 180, 5 weeks old) were reared either at 22°C for 24 h/d (thermoneutral, TN) or at 34°C for 8 h/d (heat stress, HS) and fed on one of three diets containing 0, 25 or 50 mg of capsaicinoids per kilogram for 12 weeks (2 × 3 factorial arrangement). 2. The results revealed that exposure to HS decreased feed consumption by 10.7% and egg production by 13.6%, increased serum and ovary malondialdehyde (MDA) levels by 66...
April 2017: British Poultry Science
https://www.readbyqxmd.com/read/27865412/expression-and-localization-of-matrix-metalloproteinases-mmp-2-7-9-and-their-tissue-inhibitors-timp-2-3-in-the-chicken-oviduct-during-pause-in-laying-induced-by-tamoxifen
#19
Agnieszka Leśniak-Walentyn, Anna Hrabia
Induced pause in egg laying simulates natural molting events in which the hen's reproductive organs regress and rejuvenate. Such processes require extracellular matrix remodeling that is maintained, at least in part, by the action of proteolytic enzymes known as matrix metalloproteinases (MMPs). Nevertheless, information concerning the expression and hormonal regulation of MMP system members in chickens is scarce. Therefore, MMP-2, -7, and -9 and their tissue inhibitors (TIMP-2, -3) expression and localization were investigated in all segments of the domestic hen oviduct (infundibulum, magnum, isthmus, shell gland, vagina) during a pause in egg laying induced by tamoxifen (TMX)-an estrogen receptor modulator...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27821159/ceratonia-siliqua-pod-extract-ameliorates-schistosoma-mansoni-induced-liver-fibrosis-and-oxidative-stress
#20
Ebtesam M Al-Olayan, Manal F El-Khadragy, Reem A Alajmi, Mohamed S Othman, Amira A Bauomy, Shaimaa R Ibrahim, Ahmed E Abdel Moneim
BACKGROUND: Schistosomiasis is a prevalent parasitic disease found predominantly in tropical and sub-tropical areas of the developing world, with the second highest socioeconomic and public health burden despite strenuous control efforts. In the present study, we aimed to investigate the ameliorative effects of Ceratonia siliqua pod extract (CPE) on liver fibrosis and oxidative stress in mice infected with Schistosoma mansoni. METHODS: The schistosomal hepatopathologic mouse model was established by tail immersion with schistosomal cercaria...
November 8, 2016: BMC Complementary and Alternative Medicine
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