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https://www.readbyqxmd.com/read/27916895/validation-of-folate-enriched-eggs-as-a-functional-food-for-improving-folate-intake-in-consumers
#1
Leslie Altic, Helene McNulty, Leane Hoey, Liadhan McAnena, Kristina Pentieva
Functional foods enriched with folate may be beneficial as a means of optimizing folate status in consumers. We recently developed novel eggs enriched with folate through folic acid supplementation of the hen's feed, but their potential to influence consumer folate status is unknown because the natural folate forms incorporated into the eggs may not necessarily be retained during storage and cooking. This study aimed to determine the stability of natural folates in folate-enriched eggs under typical conditions of storage and cooking...
November 30, 2016: Nutrients
https://www.readbyqxmd.com/read/27896806/nitric-oxide-synthase-during-early-embryonic-development-in-silkworm-bombyx-mori-gene-expression-enzyme-activity-and-tissue-distribution
#2
Ryo Kitta, Marina Kuwamoto, Yumi Yamahama, Keisuke Mase, Hiroshi Sawada
To elucidate the mechanism for embryonic diapause or the breakdown of diapause in Bombyx mori, we biochemically analyzed nitric oxide synthase (NOS) during the embryogenesis of B. mori. The gene expression and enzyme activity of B. mori NOS (BmNOS) were examined in diapause, non-diapause, and HCl-treated diapause eggs. In the case of HCl-treated diapause eggs, the gene expression and enzyme activity of BmNOS were induced by HCl treatment. However, in the case of diapause and non-diapause eggs during embryogenesis, changes in the BmNOS activity and gene expressions did not coincide except 48-60 h after oviposition in diapause eggs...
November 29, 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27869499/capsaicinoids-improve-egg-production-by-regulating-ovary-nuclear-transcription-factors-against-heat-stress-in-quail
#3
N Sahin, C Orhan, M Tuzcu, V Juturu, K Sahin
1. To examine the molecular mechanism of capsaicinoid supplementation from Capsicum extract, laying Japanese quail (n=180, 5 weeks old) were reared either at 22(o)C for 24 h/d (thermoneutral, TN) or 34(o)C for 8 h/d (heat stress, HS) and fed on one of three diets containing 0, 25 or 50 mg of capsaicinoids per kg for 12 weeks (2x3 factorial arrangement). 2. The results revealed that exposure to HS decreased feed consumption by 10.7% and egg production by 13.6%, increased serum and ovary malondialdehyde (MDA) levels by 66...
November 21, 2016: British Poultry Science
https://www.readbyqxmd.com/read/27865412/expression-and-localization-of-matrix-metalloproteinases-mmp-2-7-9-and-their-tissue-inhibitors-timp-2-3-in-the-chicken-oviduct-during-pause-in-laying-induced-by-tamoxifen
#4
Agnieszka Leśniak-Walentyn, Anna Hrabia
Induced pause in egg laying simulates natural molting events in which the hen's reproductive organs regress and rejuvenate. Such processes require extracellular matrix remodeling that is maintained, at least in part, by the action of proteolytic enzymes known as matrix metalloproteinases (MMPs). Nevertheless, information concerning the expression and hormonal regulation of MMP system members in chickens is scarce. Therefore, MMP-2, -7, and -9 and their tissue inhibitors (TIMP-2, -3) expression and localization were investigated in all segments of the domestic hen oviduct (infundibulum, magnum, isthmus, shell gland, vagina) during a pause in egg laying induced by tamoxifen (TMX)-an estrogen receptor modulator...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27821159/ceratonia-siliqua-pod-extract-ameliorates-schistosoma-mansoni-induced-liver-fibrosis-and-oxidative-stress
#5
Ebtesam M Al-Olayan, Manal F El-Khadragy, Reem A Alajmi, Mohamed S Othman, Amira A Bauomy, Shaimaa R Ibrahim, Ahmed E Abdel Moneim
BACKGROUND: Schistosomiasis is a prevalent parasitic disease found predominantly in tropical and sub-tropical areas of the developing world, with the second highest socioeconomic and public health burden despite strenuous control efforts. In the present study, we aimed to investigate the ameliorative effects of Ceratonia siliqua pod extract (CPE) on liver fibrosis and oxidative stress in mice infected with Schistosoma mansoni. METHODS: The schistosomal hepatopathologic mouse model was established by tail immersion with schistosomal cercaria...
November 8, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27810261/effect-of-paecilomyces-lilacinus-trichoderma-harzianum-and-trichoderma-virens-fungal-extracts-on-the-hatchability-of-ancylostoma-eggs
#6
Bianca Delgado Menezes Hofstätter, Anelise Oliveira da Silva Fonseca, Fernando de Souza Maia Filho, Julia de Souza Silveira, Beatriz Maroneze Persici, Luciana Pötter, Andressa Silveira, Zaida Inês Antoniolli, Daniela Isabel Brayer Pereira
BACKGROUND: Ancylostoma species have demanded attention due to their zoonotic potential. The use of anthelmintics is the usual method to prevent environmental contamination by Ancylostoma eggs and larvae. Nematophagous fungi have been widely used in their biological control due to the fungus ability to capture and digest free nematode forms. AIMS: The aim of this study was to evaluate the effect of four different fungal extracts of Paecilomyces lilacinus (n=2), Trichoderma harzianum (n=1) and Trichoderma virens (n=1) isolates on the hatchability of Ancylostoma eggs...
October 31, 2016: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/27798285/endocannabinoid-system-and-pregnancy
#7
REVIEW
Fernando Correa, Manuel L Wolfson, Paula Valchi, Julieta Aisemberg, Ana María Franchi
The endocannabinoid system (eCS), is a complex system, comprising the main endogenous ligands anandamide and 2-arachidonoyl glycerol, the cannabinoid receptors CB1 and CB2 and the biosynthetic and degrading enzymes. Cumulative evidence shows that the eCS plays an important role in reproduction, from egg fertilization to parturition. Therefore, alterations in this system, either by recreation/therapeutic use of cannabis or deregulation of the endogenous cannabinoids, might lead to adverse pregnancy outcomes, including retardation in embryo development, poor blastocyst implantation, inhibition of decidualization, miscarriage and compromised placentation...
December 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27761283/efficiency-of-direct-microscopy-of-stool-samples-using-an-antigen-specific-adhesin-test-for-entamoeba-histolytica
#8
Arzu İrvem, Kamil Özdil, Zuhal Çalışkan, Muhterem Yücel
BACKGROUND: E. histolytica is among the common causes of acute gastroenteritis. The pathogenic species E. histolytica and the nonpathogenic species E. dispar cannot be morphologically differentiated, although correct identification of these protozoans is important for treatment and public health. In many laboratories, the screening of leukocytes, erythrocytes, amoebic cysts, trophozoites and parasite eggs is performed using Native-Lugol's iodine for pre-diagnosis. AIMS: In this study, we aimed to investigate the frequency of E...
September 2016: Balkan Medical Journal
https://www.readbyqxmd.com/read/27744603/single-and-combined-impacts-of-vitamin-a-and-selenium-in-diet-on-productive-performance-egg-quality-and-some-blood-parameters-of-laying-hens-during-hot-season
#9
Mohamed E Abd El-Hack, Khalid Mahrose, Ali A Askar, Mahmoud Alagawany, Muhammad Arif, Muhammad Saeed, Farzana Abbasi, Rab Nawaz Soomro, Farman Ali Siyal, Maria Tabassum Chaudhry
A study was conducted using 162 Bovans laying hens to investigate the impacts of extra dietary vitamin A (0, 8000, 16,000 IU/kg), selenium (0, 0.25, 0.50 mg/kg), and their combinations on the performance, egg quality, and blood biological parameters of laying hens during summer months. Supplemental vitamin A up to 16,000 IU/kg diet significantly (P < 0.05) improved all productive traits studied except feed intake which increased with 8000 IU/kg diet compared with control. Feed intake and feed conversion of hens fed diet supplemented with selenium revealed high statistical (P = 0...
October 15, 2016: Biological Trace Element Research
https://www.readbyqxmd.com/read/27664643/changes-in-the-antigenicity-and-allergenicity-of-ovalbumin-in-chicken-egg-white-by-n-acetylglucosaminidase
#10
Ho-Young Park, Taek Joon Yoon, Ha Hyung Kim, Young Shin Han, Hee-Don Choi
Ovalbumin (OVA), an (hen) egg allergen, is one of the most abundant glycoprotein allergens associated with IgE-mediated hypersensitivity through the T-helper type 2 immune response. The effect of deglycosylation of the N-terminal glycan in OVA on allergenicity and antigenicity after N-acetylglucosaminidase treatment was studied. N-acetylglucosaminidase-treated OVA (N-OVA) evaluated using an enzyme-linked immunosorbent assay, respectively. N-OVA significantly (p<0.05) OVA-specific IgE and histamine levels...
February 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27616647/reproductive-toxicity-of-azoxystrobin-to-adult-zebrafish-danio-rerio
#11
Fangjie Cao, Lizhen Zhu, Hui Li, Song Yu, Chengju Wang, Lihong Qiu
In the past few decades, extensive application of azoxystrobin has led to great concern regarding its adverse effects on aquatic organisms. The objective of the present study was to evaluate the reproductive toxicity of azoxystrobin to zebrafish. After adult zebrafish of both sexes were exposed to 2, 20 and 200 μg/L azoxystrobin for 21 days, egg production, the fertilization rate, the gonadosomatic index (GSI) and hepatosomatic index (HSI), 17β-estradiol (E2), testosterone (T) and vitellogenin (Vtg) concentrations, and histological alterations in the gonads and livers were measured...
September 8, 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27556732/microfiltration-of-enzyme-treated-egg-whites-for-accelerated-detection-of-viable-salmonella-topic-food-science
#12
Seockmo Ku, Eduardo Ximenes, Thomas Kreke, Kirk Foster, Amanda J Deering, Michael R Ladisch
We report detection of less than 13 CFU of Salmonella per 25 g egg white within 7 hours by concentrating the bacteria using microfiltration through 0.2 μm cutoff polyethersulfone hollow fiber membranes. A combination of enzyme treatment, controlled cross-flow on both sides of the hollow fibers, and media selection were key to controlling membrane fouling so that rapid concentration and the subsequent detection of low numbers of microbial cells were achieved. We leveraged the protective effect of egg white proteins and peptone so that the proteolytic enzymes did not attack the living cells while hydrolyzing the egg white proteins responsible for fouling...
August 24, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27540477/recent-advances-in-pathogenesis-assessment-and-treatment-of-atherosclerosis
#13
REVIEW
J David Spence
In recent years, there have been a number of advances in the pathogenesis and treatment of atherosclerosis and in assessing prognosis in carotid atherosclerosis. Risk stratification to improve vascular prevention by identifying patients most likely to benefit from intensive therapy is much improved by measuring carotid plaque burden. In patients with asymptomatic carotid stenosis, a number of modalities can be used to identify the 10-15% who could benefit from endarterectomy or stenting. Transcranial Doppler embolus detection, echolucency and ulceration on 3D ultrasound, intraplaque hemorrhage on magnetic resonance imaging (MRI), and reduced cerebrovascular reserve are useful already; new approaches including plaque texture on ultrasound and imaging of plaque inflammation and early calcification on positron emission tomography/computed tomography (PET/CT) are in development...
2016: F1000Research
https://www.readbyqxmd.com/read/27454107/antischistosomal-activity-of-hederacochiside-c-against-schistosoma-japonicum-harbored-in-experimentally-infected-animals
#14
Nai-Xin Kang, Yuan-Jian Zhu, Jian-Ping Zhao, Wei-Feng Zhu, Yan-Li Liu, Qiong-Ming Xu, Hong-Xiang Zhuge, Ikhlas A Khan, Shi-Lin Yang
The present study was undertaken to investigate whether hederacochiside C (HSC) possesses antischistosomal effects and anti-inflammatory response activities in Schistosoma japonicum-infected mice. Different concentrations of HSC were administrated to the mice infected by schistosomula or adult worm by intravenous injection twice a day for five consecutive days. The total worm burden, female worm burden, and the egg burden in liver of mice treated with 400 mg/kg HSC were fewer than those in non-treated ones...
July 25, 2016: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/27443607/anti-dengue-efficacy-of-bioactive-andrographolide-from-andrographis-paniculata-lamiales-acanthaceae-against-the-primary-dengue-vector-aedes-aegypti-diptera-culicidae
#15
Edward-Sam Edwin, Prabhakaran Vasantha-Srinivasan, Sengottayan Senthil-Nathan, Annamalai Thanigaivel, Athirstam Ponsankar, Venkatraman Pradeepa, Selvaraj Selin-Rani, Kandaswamy Kalaivani, Wayne B Hunter, Ahmed Abdel-Megeed, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi
The current study investigated the toxic effect of the leaf extract compound andrographolide from Andrographis paniculata (Burm.f) against the dengue vector Ae. aegypti. GC-MS analysis revealed that andrographolide was recognized as the major chemical constituent with the prominent peak area compared with other compounds. All isolated toxic compounds were purified and confirmed through RP-HPLC against chemical standards. The larvicidal assays established at 25ppm of bioactive compound against the treated instars of Ae...
November 2016: Acta Tropica
https://www.readbyqxmd.com/read/27396165/-the-isolation-and-identification-of-infectious-bronchitis-virus-ptfy-strain-in-muscovy-ducks
#16
Xiaoping Wu, Shulei Pan, Wuduo Zhou, Yijiang Wu, Yifan Huang, Baocheng Wu
In July 2009, some farms of breeding Muscovy ducks on the peak of egg laying suffered the decrease of hatching rate and the quality of the eggs showing low mortality and no evident respiratory symptoms. The swelling and congestive ovary was visible after autopsy. This study was brought out for the diagnosis of these cases. The virus was isolated and identified by the methods of virus culture in chicken embryo, physical and chemical properties test, hemagglutinin test, NDV (Newcastle diseases Virus) interference test, electron microscope observation, pathogenicity test and the gene sequence analysis...
March 2016: Bing du Xue Bao, Chinese Journal of Virology
https://www.readbyqxmd.com/read/27324075/effects-of-triploidy-induction-on-antioxidant-defense-status-in-rainbow-trout-oncorhynchus-mykiss-during-early-development
#17
Kaveh Taghipoor, Saeed Keyvanshokooh, Amir Parviz Salati, Hossein Pasha-Zanoosi, Samad Bahrami Babaheydari
The objective of the present study was to examine the antioxidant status of rainbow trout (Oncorhynchus mykiss) during the early stages of development (fertilized egg, eyed egg, alevin and fry) as an effect of triploidy induction. Eggs and milt were taken from eight females and six males. After insemination, the eggs were incubated at 10°C for 10min. Half of the fertilized eggs were then subjected to heat-shock for 10min submerged in a 28°C water bath to induce triploidy. The remainder were incubated normally and used as diploid controls...
August 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/27272703/long-term-effects-of-antibiotics-norfloxacin-and-sulfamethoxazole-in-a-partial-life-cycle-study-with-zebrafish-danio-rerio-effects-on-growth-development-and-reproduction
#18
Zhenhua Yan, Guanghua Lu, Qiuxia Ye, Jianchao Liu
A partial life-cycle study with zebrafish (Danio rerio) was conducted to evaluate the long-term effects of antibiotics, norfloxacin (NOR) and sulfamethoxazole (SMX). A series of bio-endpoints correlated to the growth, development, and reproduction was assessed. The results showed that the body weight and the condition factor were depressed by SMX at 200 μg/L during the growth period. Meanwhile, the activities of metabolic enzyme (ethoxyresorufin O-deethylase, EROD) and antioxidant enzymes (superoxide dismutase, SOD and catalase, CAT) were stimulated in all cases...
September 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27194251/the-effects-of-cadmium-exposure-on-fitness-related-traits-and-antioxidant-responses-in-the-wolf-spider-pardosa-pseudoannulata
#19
Chang-Chun Li, Guo-Yuan Li, Yue-Li Yun, Jian Chen, Zeng-Tao Zhang, Yu Peng
The objective of the present study was to assess the ecotoxicological responses of Pardosa pseudoannulata to a common environmental pollutant, cadmium. Third-instar spiderlings and adult spiders were exposed to sublethal concentrations of CdCl2 solution in their drinking water. The Cd content in P. pseudoannulata adults increased significantly with the number of days of exposure to a 0.2 mM CdCl2 solution, when exposed to 2 mM CdCl2 solution, the Cd content in the spiders increased sharply in the first two (male) or three (female) weeks, and then no significant changes were recorded following with the next three (male) or two (female) weeks exposure...
July 2016: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/27178210/significant-reduction-in-allergenicity-of-ovalbumin-from-chicken-egg-white-following-treatment-with-ascidian-viscera-n-acetylglucosaminidase
#20
Hye Seong Hwang, Heajin Park, Jihye Kim, Jai Yeon Choi, Young Kwang Lee, Ho-Young Park, Hee-Don Choi, Ha Hyung Kim
Ovalbumin (OA) is the most abundant ingredient of chicken egg-white allergenic proteins. In the present study we investigated the possibility of reducing OA allergenicity by treatment with a natural protein exhibiting N-acetylglucosaminidase (NA) activity. Ascidian is cultivated as a food resource in northeast Asia. The ascidian viscera NA (AVNA) with almost no other exoglycosidases or proteolytic enzymes was isolated by applying size-exclusion chromatography to a protein precipitate of ascidian viscera. Intact OA was mixed with AVNA containing 0...
June 17, 2016: Biochemical and Biophysical Research Communications
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