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https://www.readbyqxmd.com/read/28916657/tenascin-c-and-integrin-%C3%AE-9-mediate-interactions-of-prostate-cancer-with-the-bone-microenvironment
#1
Rebeca San Martin, Ravi Pathak, Antrix Jain, Sung Yun Jung, Susan G Hilsenbeck, Maria C Piňa-Barba, Andrew G Sikora, Kenneth J Pienta, David R Rowley
Deposition of the extracellular matrix protein tenascin-C is part of the reactive stroma response, which has a critical role in prostate cancer progression. Here we report that tenascin-C is expressed in the bone endosteum and involved associated with formation of prostate bone metastases. Metastatic cells cultured on osteo-mimetic surfaces coated with tenascin-C exhibited enhanced adhesion and colony formation as mediated by integrin α9β1. Additionally, metastatic cells preferentially migrated and colonized tenascin-C-coated trabecular bone xenografts in a novel system that employed chorioallantoic membranes of fertilized chicken eggs as host...
September 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28889977/novel-organelles-with-elements-of-bacterial-and-eukaryotic-secretion-systems-weaponize-parasites-of-drosophila
#2
Mary Ellen Heavner, Johnny Ramroop, Gwenaelle Gueguen, Girish Ramrattan, Georgia Dolios, Michael Scarpati, Jonathan Kwiat, Sharmila Bhattacharya, Rong Wang, Shaneen Singh, Shubha Govind
The evolutionary success of parasitoid wasps, a highly diverse group of insects widely used in biocontrol, depends on a variety of life history strategies in conflict with those of their hosts [1]. Drosophila melanogaster is a natural host of parasitic wasps of the genus Leptopilina. Attack by L. boulardi (Lb), a specialist wasp to flies of the melanogaster group, activates NF-κB-mediated humoral and cellular immunity. Inflammatory blood cells mobilize and encapsulate Lb eggs and embryos [2-5]. L. heterotoma (Lh), a generalist wasp, kills larval blood cells and actively suppresses immune responses...
September 6, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28862340/in-vitro-reconstitution-of-the-endoplasmic-reticulum
#3
Csilla-Maria Ferencz, Gernot Guigas, Andreas Veres, Brigitte Neumann, Olaf Stemmann, Matthias Weiss
Reconstitution of cellular organelles in vitro offers the possibility to perform quantitative and qualitative experiments in a controlled environment that cannot be done with the same accuracy in living cells. Following a previous report, the subsequent list of protocols describes how to reconstitute and quantify a tubular ER network in vitro based on purified microsomes from culture cells and cytosol from Xenopus laevis egg extracts. Biological material preparation and reconstitution assays require mostly basic laboratory instrumentation and chemicals, and can be executed without any specific training, making them appealing to a wide range of laboratories...
September 1, 2017: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/28853253/-establishment-of-a-high-sensitive-indirect-elisa-for-detecting-specific-antibodies-against-h9-subtype-avian-influenza-virus
#4
Wei Zhang, Lidan Hou, Jie Song, Shuang Zhang, Yun Li, Jing Li, Lei Sun, Wenhui Fan, Wenjun Liu
H9 subtype avian influenza virus causes worldwide epidemic, resulting in enormous economic losses of poultry production. In the present study, an indirect ELISA method was established for more accurate and specific detection. The recombinant protein of the globular head domain of HA of H9 subtype avian influenza virus was used as antigen. Specific blocking buffers and dilution buffers were determined to increase the sensitivity and specificity. The sensitivity of ELISA was higher than that of hemagglutination inhibition (HI) test...
August 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28818776/bacillus-thuringiensis-coated-zinc-oxide-nanoparticle-and-its-biopesticidal-effects-on-the-pulse-beetle-callosobruchus-maculatus
#5
Balasubramanian Malaikozhundan, Baskaralingam Vaseeharan, Sekar Vijayakumar, Merlin P Thangaraj
Insect pests belonging to the genus Callosobruchus are the major cause of damage to stored pulse crops. Recently, nanotechnology has emerged as a promising tool for pest control. In the present study, we report for the first time the synthesis and biological evaluation of Bacillus thuringiensis coated zinc oxide nanoparticles (Bt-ZnO NPs) on the pulse beetle, Callosobruchus maculatus. The biologically synthesized Bt-ZnO NPs were extensively characterized using UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Zeta potential...
August 9, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28807296/myiasis-of-domestic-and-wild-ruminants-caused-by-hypodermatinae-in-the-mediterranean-and-indian-subcontinent
#6
REVIEW
Anish Yadav, Rosario Panadero, Rajesh Katoch, Rajesh Godara, Eva Cabanelas
Myiasis caused by Hypodermatinae flies is an economically important disease affecting domesticated and wild ruminants in countries of the Mediterranean and Indian subcontinent. The adult flies have a short life span of 1 week, and they spread disease by laying their eggs on the coat of animals. Hypoderma spp. primarily lay their eggs on cattle, buffalo, roe deer, red deer and reindeer, while Przhevalskiana spp. lay eggs on the coat of goats. The larvae live as tissue parasites, inducing myiasis in the host, and have a major impact on the productivity and welfare of infested animals...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28804244/development-of-new-contrast-agents-for-imaging-function-and-metabolism-by-magnetic-resonance-imaging
#7
Alexandra Carvalho, M Clara Gonçalves, M Luísa Corvo, M Bárbara F Martins
Liposomes are interesting nanosystems with a wide range of medical application. One particular application is their ability to enhance contrast in magnetic resonance images; when properly loaded with magnetic/superparamagnetic nanoparticles, this means to act as contrast agents. The design of liposomes loaded with magnetic particles, magnetoliposomes, presents a large number of possibilities depending on the application from image function to metabolism. More interesting is its double function application as theranostics (diagnostics and therapy)...
2017: Magnetic Resonance Insights
https://www.readbyqxmd.com/read/28796817/histological-study-on-maturation-fertilization-and-the-state-of-gonadal-region-following-spawning-in-the-model-sea-anemone-nematostella-vectensis
#8
Elizabeth Moiseeva, Claudette Rabinowitz, Guy Paz, Baruch Rinkevich
The starlet sea-anemone Nematostella vectensis has emerged as a model organism in developmental biology. Still, our understanding of various biological features, including reproductive biology of this model species are in its infancy. Consequently, through histological sections, we study here key stages of the oogenesis (oocyte maturation/fertilization), as the state of the gonad region immediately after natural spawning. Germ cells develop in a secluded mesenterial gastrodermal zone, where the developing oocytes are surrounded by mucoid glandular cells and trophocytes (accessory cells)...
2017: PloS One
https://www.readbyqxmd.com/read/28764459/electrically-conductive-synthetic-vocal-fold-replicas-for-voice-production-research
#9
Kyle L Syndergaard, Shelby Dushku, Scott L Thomson
A method of fabricating electrically conductive synthetic vocal fold replicas and monitoring their vibration via resistance measurement is presented. Normally non-conductive silicone replicas were coated with conductive graphite and subjected to long-term vibration tests. Synchronized resistance and imaging data using hemilarynx and full larynx configurations showed an inverse correlation between replica contact area and resistance during vibration, similar to clinical electroglottography (EGG) used to estimate vocal fold contact area...
July 2017: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/28748836/morphometric-and-morphological-appraisal-of-the-eggs-of-anopheles-stephensi-diptera-culicidae-from-india
#10
Varun Tyagi, Sunil Dhiman, Ajay K Sharma, A R Srivastava, Bipul Rabha, D Sukumaran, Vijay Veer
BACKGROUND & OBJECTIVES: Anopheles stephensi is one of the most important urban malaria vectors in India and contribute about 12% of total malaria cases. An. stephensi has three ecological variants; type, intermediate and mysorensis that can be differentiated on the basis of differences in number of ridges on egg float and on the basis of spiracular indices. Because of its anthropophilic nature the 'type' form is an efficient malaria vector. In the present study, the egg surface morphometry and morphology of An...
April 2017: Journal of Vector Borne Diseases
https://www.readbyqxmd.com/read/28720818/structural-and-rheological-properties-conferring-fertilization-competence-to-xenopus-egg-coating-envelope
#11
Mayu Hanaue, Naofumi Miwa
The extracellular egg-coating envelope that comprises a meshwork of filaments polymerized by glycoproteins plays a pivotal role in species-selective sperm recognition and subsequent fertilization; however, the structural and rheological properties conferring fertilization competence to the egg-coating envelope remain poorly unveiled. Here we show several nanoscale-structural and viscoelastic properties of the egg-coat using the transmission electron microscopy and the quartz crystal microbalance experiments, following clamp of the egg-coat at either fertilization-competent or -incompetent statuses by short-term pretreatment with synthetic peptides...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28695569/in-vitro-evidence-for-the-participation-of-drosophila-melanogaster-sperm-%C3%AE-n-acetylglucosaminidases-in-the-interactions-with-glycans-carrying-terminal-n-acetylglucosamine-residues-on-the-egg-s-envelopes
#12
Jari Intra, Concetta Veltri, Daniela De Caro, Maria Elisa Perotti, Maria Enrica Pasini
Fertilization is a complex and multiphasic process, consisting of several steps, where egg-coating envelope's glycoproteins and sperm surface receptors play a critical role. Sperm-associated β-N-acetylglucosaminidases, also known as hexosaminidases, have been identified in a variety of organisms. Previously, two isoforms of hexosaminidases, named here DmHEXA and DmHEXB, were found as intrinsic proteins in the sperm plasma membrane of Drosophila melanogaster. In the present work, we carried out different approaches using solid-phase assays in order to analyze the oligosaccharide recognition ability of D...
September 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28675377/investigation-of-novel-superparamagnetic-ni0-5zn0-5fe2o4-albumen-nanoparticles-for-controlled-delivery-of-anticancer-drug
#13
Mohd Qasim, Khushnuma Asghar, Gangappa Dharmapuri, D Das
In the present work, multifunctional Ni0.5Zn0.5Fe2O4@albumen (NZF@Alb) and doxorubicin-loaded Ni0.5Zn0.5Fe2O4@albumen (NZF@Alb-Dox) core-shell nanoparticles have been prepared by a green and simple method using inexpensive chicken egg albumen and have been characterized for different physiochemical properties. The structural, morphological, thermal, and magnetic properties of the prepared nanoparticles have been investigated by an x-ray diffractometer, high-resolution transmission electron microscopy (HRTEM), field emission scanning electron microscopy, Fourier-transformed infrared, thermogravimetric analysis, and vibrating sample magnetometer techniques...
July 4, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28656787/quality-assessment-of-chicken-eggshell-cuticle-by-infrared-spectroscopy-and-staining-techniques-a-comparative-study
#14
N Dominguez-Gasca, A Muñoz, A B Rodriguez-Navarro
1. The cuticle is a very thin organic layer that coats the eggshell surface and plugs the eggshell pores preventing bacterial penetration. It also reduces egg shell permeability which is important to maintain egg internal quality. Thus, the eggshell cuticle quality is crucial to ensure the food safety and quality of eggs. 2. A new methodology to assess eggshell cuticle quality, based on attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), with a more traditional method based on cuticle staining techniques...
June 28, 2017: British Poultry Science
https://www.readbyqxmd.com/read/28622512/structural-basis-of-egg-coat-sperm-recognition-at-fertilization
#15
Isha Raj, Hamed Sadat Al Hosseini, Elisa Dioguardi, Kaoru Nishimura, Ling Han, Alessandra Villa, Daniele de Sanctis, Luca Jovine
Recognition between sperm and the egg surface marks the beginning of life in all sexually reproducing organisms. This fundamental biological event depends on the species-specific interaction between rapidly evolving counterpart molecules on the gametes. We report biochemical, crystallographic, and mutational studies of domain repeats 1-3 of invertebrate egg coat protein VERL and their interaction with cognate sperm protein lysin. VERL repeats fold like the functionally essential N-terminal repeat of mammalian sperm receptor ZP2, whose structure is also described here...
June 15, 2017: Cell
https://www.readbyqxmd.com/read/28622504/sex-at-atomic-resolution
#16
COMMENT
Jae Yeon Hwang, Jean-Ju Chung
Interspecies fertilization is rare, partly due to species separation enforced at the molecular level. In this issue, Raj et al. now reveal the crystal structures of mollusk egg coat protein, VERL, complexed with cognate sperm protein lysin. Given that VERL is structurally similar to mammalian ZP2, the mechanism elucidating species-specific gamete recognition likely exists in mammals.
June 15, 2017: Cell
https://www.readbyqxmd.com/read/28580709/early-ovariectomy-reveals-the-germline-encoding-of-natural-anti-a-and-tn-cross-reactive-immunoglobulin-m-igm-arising-from-developmental-o-galnac-glycosylations-germline-encoded-natural-anti-a-tn-cross-reactive-igm
#17
Peter Arend
While native blood group A-like glycans have not been demonstrated in prokaryotic microorganisms as a source of human "natural" anti-A isoagglutinin production, and metazoan eukaryotic N-acetylgalactosamine O-glycosylation of serine or threonine residues (O-GalNAc-Ser/Thr-R) does not occur in bacteria, the O-GalNAc glycan-bearing ovarian glycolipids, discovered in C57BL/10 mice, are complementary to the syngeneic anti-A-reactive immunoglobulin M (IgM), which is not present in animals that have undergone ovariectomy prior to the onset of puberty...
July 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28544760/a-novel-pattern-of-yolk-processing-in-developing-snake-eggs-colubridae-lampropeltini-and-its-functional-and-evolutionary-implications
#18
Kathryn G Powers, Daniel G Blackburn
Early amniotic vertebrates evolved large-yolked eggs that permitted production of well-developed, terrestrial hatchlings. This reproductive pattern required new mechanisms for cellularizing the yolk and mobilizing it for embryonic use. In birds, cells that line the yolk sac cavity phagocytose and digest the yolk material, a pattern that is commonly assumed to be universal among oviparous amniotes. However, recent evidence challenges the assumption that all squamate reptiles conform to the avian developmental pattern...
May 24, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28499203/efficient-protein-digestion-using-immobilized-trypsin-onto-tannin-modified-fe3o4-magnetic-nanoparticles
#19
Keziban Atacan, Bekir Çakıroğlu, Mahmut Özacar
Fe3O4 magnetic nanoparticles (MNPs) were prepared via solvothermal method. A commercially available trypsin was covalently immobilized onto MNPs modified with tannin (T) via a novel binding process. The morphology, structure, surface and magnetic properties of the obtained nanostructures were characterized comprehensively. The Fe3O4 MNPs had a saturation magnetization value of 60.18emu/g at room temperature, while the tannin modified Fe3O4 MNPs, and the trypsin immobilized on tannin modified-Fe3O4 MNPs possessed a saturation magnetization value of 57...
May 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28403915/the-egg-coat-zona-pellucida-3-glycoprotein-evolution-of-its-putative-sperm-binding-region-in-old-world-murine-rodents-rodentia-muridae
#20
Christine A Swann, Steven J B Cooper, William G Breed
In eutherian mammals, before fertilisation can occur the spermatozoon has to bind to, and penetrate, the egg coat, the zona pellucida (ZP). In the laboratory mouse there is good evidence that the primary sperm-binding site is a protein region encoded by Exon 7 of the ZP3 gene and it has been proposed that binding is species specific and evolves by sexual selection. In the present study we investigate these hypotheses by comparing Exon 6 and 7 sequences of ZP3 in 28 species of murine rodents of eight different divisions from Asia, Africa and Australasia, in which a diverse array of sperm morphologies occurs...
April 13, 2017: Reproduction, Fertility, and Development
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