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https://www.readbyqxmd.com/read/29761641/uhplc-ms-ms-method-for-determining-dinotefuran-and-its-main-metabolites-in-samples-of-plants-animal-derived-foods-soil-and-water
#1
Ying Zhang, Xiaohu Wu, Tingting Duan, Jun Xu, Fengshou Dong, Xingang Liu, Xiaogang Li, Pengqiang Du, Yongquan Zheng
An ultra high performance liquid chromatography with tandem triple quadrupole mass spectrometry residue method for the quantification and identification of dinotefuran and its main metabolites 1-methyl-3-(tetrahydro-3-furylmethyl) urea and 1-methyl-3-(tetrahydro-3-furylmethyl) guanidine in fruit (watermelon), vegetable (cucumber), cereal (rice), animal-derived foods (milk, egg, and pork), soil and water was developed and validated. The samples were extracted with acetonitrile containing 15% v/v acetic acid and purified with dispersive solid-phase extraction with octadecylsilane, primary secondary amine, graphitized carbon black, or zirconia-coated silica prior to analysis...
May 15, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29715032/nanoplasmonic-sensing-and-capillary-electrophoresis-for-fast-screening-of-interactions-between-phosphatidylcholine-biomembranes-and-surfactants
#2
Filip Dusa, Wen Chen, Joanna Witos, Susanne Kristina Wiedmer
Nanoplasmonic sensing (NPS), based on localized surface plasmon resonance, with sensors composed of glass covered with golden nanodisks and overlaid with a SiO2 coating was applied in this study. Egg phosphatidylcholine (eggPC), being an easily accessible membrane-forming lipid, was used for preparation of biomimicking membranes. Small unilamellar vesicles with an approximate hydrodynamic diameter of 30 nm, formed by sonication in HEPES buffer, were adsorbed within 10 min on the sensor surface either as intact vesicles or as a planar bilayer...
May 1, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29710633/endocrine-disruptors-in-breeding-ponds-and-reproductive-health-of-toads-in-agricultural-urban-and-natural-landscapes
#3
Veronika Bókony, Bálint Üveges, Nikolett Ujhegyi, Viktória Verebélyi, Edina Nemesházi, Olivér Csíkvári, Attila Hettyey
Many chemical pollutants have endocrine disrupting effects which can cause lifelong reproductive abnormalities in animals. Amphibians are the most threatened group of vertebrates, but there is little information on the nature and quantity of pollutants occurring in typical amphibian breeding habitats and on the reproductive capacities of amphibian populations inhabiting polluted areas. In this study we investigated the occurrence and concentrations of endocrine disrupting chemicals in the water and sediment of under-studied amphibian breeding habitats in natural, agricultural and urbanized landscapes...
April 13, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29674376/cherchez-la-femme-impact-of-ocean-acidification-on-the-egg-jelly-coat-and-attractants-for-sperm
#4
Shawna A Foo, Dione Deaker, Maria Byrne
The impact of ocean acidification on marine invertebrate eggs and consequences for sperm chemotaxis are unknown. In the sea urchins Heliocidaris tuberculata and H. erythrogramma , with small (93µm) and large (393µm) eggs, respectively, we documented the effect of decreased pH on the egg jelly coat, an extracellular matrix that increases target size for sperm and contains sperm attracting molecules. In near future conditions (pH 7.8, 7.6) the jelly coat of H. tuberculata decreased by 11 and 21%, reducing egg target size by 9 and 17%, respectively...
April 19, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29600280/reproductive-system-symbiotic-bacteria-are-conserved-between-two-distinct-populations-of-euprymna-scolopes-from-oahu-hawaii
#5
Allison H Kerwin, Spencer V Nyholm
Female Hawaiian bobtail squid, Euprymna scolopes, harbor a symbiotic bacterial community in a reproductive organ, the accessory nidamental gland (ANG). This community is known to be stable over several generations of wild-caught bobtail squid but has, to date, been examined for only one population in Maunalua Bay, Oahu, HI. This study assessed the ANG and corresponding egg jelly coat (JC) bacterial communities for another genetically isolated host population from Kaneohe Bay, Oahu, HI, using 16S amplicon sequencing...
March 2018: MSphere
https://www.readbyqxmd.com/read/29594602/preparation-of-magnetic-molecularly-imprinted-polymers-based-on-a-deep-eutectic-solvent-as-the-functional-monomer-for-specific-recognition-of-lysozyme
#6
Kaijia Xu, Yuzhi Wang, Xiaoxiao Wei, Jing Chen, Panli Xu, Yigang Zhou
A magnetized molecularly imprinted polymer (MIP) was prepared via a surface-imprinting technique. An allyl-based deep eutectic solvent was chosen as the functional monomer to obtain the polymer for specific recognition of lysozyme. It was deposited on silica-coated magnetite nanoparticles. The structure of the polymer was confirmed by X-ray diffraction, Fourier transform infrared spectrometry, transmission electron microscopy, thermogravimetric analysis and vibrating sample magnetometry. The maximum binding capacity of the imprinted polymer is found to be 108 mg·g-1 , which is higher than that of non-imprinted polymer...
January 31, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29594583/impedimetric-detection-of-bacteria-by-using-a-microfluidic-chip-and-silver-nanoparticle-based-signal-enhancement
#7
Renjie Wang, Yi Xu, Thomas Sors, Joseph Irudayaraj, Wen Ren, Rong Wang
The authors describe a method that can significantly improve the performance of impedimetric detection of bacteria. A multifunctional microfluidic chip was designed consisting of interdigitated microelectrodes and a micro-mixing zone with a Tesla structure. This maximizes the coating of bacterial surfaces with nanoparticles and results in improved impedimetric detection. The method was applied to the detection of Escherichia coli O157:H7 (E. coli). Silver enhancement was accomplished by coating E.coli with the cationic polymer diallyldimethylammonium chloride (PDDA) to form positively charged E...
February 19, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29576478/mammalian-oocytes-locally-remodel-follicular-architecture-to-provide-the-foundation-for-germline-soma-communication
#8
Stephany El-Hayek, Qin Yang, Laleh Abbassi, Greg FitzHarris, Hugh J Clarke
Germ cells develop in a microenvironment created by the somatic cells of the gonad [1-3]. Although in males, the germ and somatic support cells lie in direct contact, in females, a thick extracellular coat surrounds the oocyte, physically separating it from the somatic follicle cells [4]. To bypass this barrier to communication, narrow cytoplasmic extensions of the follicle cells traverse the extracellular coat to reach the oocyte plasma membrane [5-9]. These delicate structures provide the sole platform for the contact-mediated communication between the oocyte and its follicular environment that is indispensable for production of a fertilizable egg [8, 10-15]...
March 8, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29485013/development-of-microemulsion-based-nabumetone-transdermal-delivery-for-treatment-of-arthritis
#9
Swati Jagdale, Gokul Deore, Anuruddha Chabukswar
Background Nabumetone is biopharmaceutics classification system (BCS) class II drug, widely used in the treatment of osteoarthritis and rheumatoid arthritis. The most frequently reported adverse reactions for the drug involve disturbance in gastrointestinal tract , diarrhea, dyspepsia and abdominal pain. Microemulgel has advantages of microemulsion for improving solubility for hydrophobic drug. Patent literature had shown that the work for drug has been carried on spray chilling, enteric coated tablet, and topical formulation which gave idea for present research work for development of transdermal delivery...
February 26, 2018: Recent Patents on Drug Delivery & Formulation
https://www.readbyqxmd.com/read/29448602/preparation-of-silica-coated-magnetic-nanoparticles-for-bioseparation
#10
Dae-Won Lee, Hira Fatima, Kyo-Seon Kim
Magnetic nanoparticles have been intensively developed and applied in several biomedical applications such as targeted delivery, drug therapy, hyperthermia, magnetic resonance imaging and bioseparation etc. This work describes a simple and convenient method to synthesize silica coated Fe3O4 nanoparticles which have the potential to be used in the bioseparation of fetuin from fetal bovine serum and albumin from the egg. In this work, uniform Fe3O4 particles were prepared through the one-pot solvothermal process at 200 °C for 12 h using a sole iron precursor (FeCl3 · 6H2O) and then were coated with SiO2 to prepare silica coated Fe3O4 nanoparticles by hydrolysis and condensation of tetraethyl orthosilicate in ethanol and H2O medium...
February 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29407938/colorimetric-aptasensors-for-determination-of-tobramycin-in-milk-and-chicken-eggs-based-on-dna-and-gold-nanoparticles
#11
Qiang Ma, Yuxian Wang, Jie Jia, Yuhong Xiang
Colorimetric aptasensors were designed for detection of tobramycin (TOB) based on unmodified gold nanoparticles (AuNPs) and single-strand DNA (ssDNA). In the absence of TOB, the DNA aptamer was coated on the surface of AuNPs to keep it against salt-induced aggregation. In the presence of TOB, aptamer will bind with TOB and detach from the surface of AuNPs because of higher affinities between aptamer and TOB. Then less protection of DNA may result in the aggregation of AuNPs by salt and an apparent color change from red to purple-blue...
May 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29385797/in-vitro-bioavailability-cellular-antioxidant-activity-and-cytotoxicity-of-%C3%AE-carotene-loaded-emulsions-stabilized-by-catechin-egg-white-protein-conjugates
#12
Luping Gu, Che Pan, Yujie Su, Ruojie Zhang, Hang Xiao, David Julian McClements, Yanjun Yang
Previously, it was shown that catechin-egg white protein (CT-EWP) conjugates were effective antioxidant emulsifiers that could form and stabilize emulsions, and also inhibit the degradation of encapsulated carotenoids. The objective of the current study was to evaluate the impact of conjugation on the in vitro bioavailability, cellular antioxidant activity, and cytotoxicity of β-carotene-loaded emulsions. Lipid droplets coated with EWP or with CT-EWP conjugates exhibited quite similar behavior when they were passed through a simulated gastrointestinal tract...
February 21, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29340252/positive-selection-on-human-gamete-recognition-genes
#13
Michael W Hart, Daryn A Stover, Vanessa Guerra, Sahar V Mozaffari, Carole Ober, Carina F Mugal, Ingemar Kaj
Coevolution of genes that encode interacting proteins expressed on the surfaces of sperm and eggs can lead to variation in reproductive compatibility between mates and reproductive isolation between members of different species. Previous studies in mice and other mammals have focused in particular on evidence for positive or diversifying selection that shapes the evolution of genes that encode sperm-binding proteins expressed in the egg coat or zona pellucida (ZP). By fitting phylogenetic models of codon evolution to data from the 1000 Genomes Project, we identified candidate sites evolving under diversifying selection in the human genes ZP3 and ZP2 ...
2018: PeerJ
https://www.readbyqxmd.com/read/29307115/bioinformatic-analyses-of-zona-pellucida-genes-in-vertebrates-and-their-expression-in-nile-tilapia
#14
Tianli Wu, Yunying Cheng, Zhilong Liu, Wenjing Tao, Shuqing Zheng, Deshou Wang
Zona pellucida (ZP) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and other vertebrates. Totally 16, 9, 17, 27, 21, 20, 26, 19, 14,11, 24, 17, 9, 18, 8, 11, 9, 8, 5, and 4 ZP genes belonging to 5 subfamilies (ZPA, ZPB, ZPC, ZPD, and ZPAX) were found in the sea lamprey, elephant shark, coelacanth, spotted gar, zebrafish, medaka, stickleback, Nile tilapia, Amazon molly, platyfish, seahorse, Northern snakehead, cavefish, tetraodon, clawed frog, turtle, chicken, platypus, kangaroo rat, and human genomes, respectively...
April 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29247381/development-of-a-light-initiated-chemiluminescent-assay-for-the-quantitation-of-sige-against-egg-white-allergens-based-on-component-resolved-diagnosis
#15
Ying Bian, Changshan Liu, Tiantian She, Mengjuan Wang, Juanjuan Yan, Dianjun Wei, Huiqiang Li
The determination of specific IgE (sIgE) level is of great importance in IgE-mediated food allergies. Our aim was to develop a homogeneous immunoassay-light-initiated chemiluminescent assay (LICA)-for measuring allergen sIgE of a single component in egg white, thus evaluating the LICA-sIgE assay as a useful tool in the diagnosis of food allergy. The LICA-sIgE assay was performed by incubating serum sample with anti-human IgE antibody coated with chemiluminescer beads, streptavidin-coated sensitizer beads, and biotinylated antigens, which consist of four components in egg white...
February 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29227533/functional-magnetic-nanoparticles-for-highly-efficient-cholesterol-removal
#16
Jun Sun, Bin Xu, Yaoyao Mu, Haile Ma, Wenjuan Qu
In this study, magnetic nanoparticles functionalized with carboxylated β-cyclodextrin (CM-β-CD; referred to Fe3 O4 @CM-β-CD) were synthesized and used for the efficient removal of cholesterol from milk and egg yolk via host-guest interactions. The results of Fourier-transform infrared, X-ray photoelectron spectroscopy, and thermogravimetric analysis indicated that the CM-β-CD was successfully conjugated to the surface of Fe3 O4 , and the amount of CM-β-CD attached on Fe3 O4 @CM-β-CD was determined to be 9...
January 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29210017/vampirolepis-kulkinae-n-sp-cyclophyllidea-hymenolepididae-from-the-common-noctule-bat-nyctalus-noctula-schreber-chiroptera-vespertilionidae-in-kazakhstan
#17
Tatiana A Makarikova
A previously unrecognised species of hymenolepidid cestode attributable to Vampirolepis Spassky, 1954 is described based on specimens from the common noctule bat Nyctalus noctula (Schreber) (Chiroptera: Vespertilionidae) from southeastern Kazakhstan (Dzungarian Alatau). Specimens of Vampirolepis kulkinae n. sp. differ from the morphologically similar congeners based on the number, size and shape of the rostellar hooks. The new species is further distinguished from additional cestodes attributed to Vampirolepis (sensu lato) by the arrangement of the testes in one row, egg structure (i...
January 2018: Systematic Parasitology
https://www.readbyqxmd.com/read/29199426/characterization-of-key-factors-of-anchovy-engraulis-japonicus-meat-in-the-nanoparticle-mediated-enhancement-of-non-heme-iron-absorption
#18
Yaqun Zou, Liang Zhao, Guangxin Feng, Yu Miao, Haohao Wu, Mingyong Zeng
Anchovy (Engraulis japonicus) meat (AM) has been shown to promote nonheme iron absorption via a ferric oxyhydroxide nanoparticle (FeONP)-mediated mechanism. Here, formulation modifications of an egg-white-based AIN-93G diet with AM fractions resulted hemoglobin regeneration efficiencies in anemic rats following an order control (23.69 ± 3.99%) < ferrous-sulfate-replacement of ferric citrate (39.89 ± 2.97%) ≈ dehemeed-AM-protein-replacement of egg white (45.88 ± 4.76%) ≈ AM-lipid-replacement of soybean oil (43...
December 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29187577/prc1e-and-kif4a-control-microtubule-organization-within-and-between-large-xenopus-egg-asters
#19
P A Nguyen, C M Field, T J Mitchison
The cleavage furrow in Xenopus zygotes is positioned by two large microtubule asters that grow out from the poles of the first mitotic spindle. Where these asters meet at the midplane, they assemble a disk-shaped interaction zone consisting of anti-parallel microtubule bundles coated with chromosome passenger complex (CPC) and centralspindlin that instructs the cleavage furrow. Here we investigate the mechanism that keeps the two asters separate and forms a distinct boundary between them, focusing on the conserved cytokinesis midzone proteins Prc1 and Kif4A...
February 1, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29143139/effect-of-protein-adsorption-layers-and-solution-treatments-on-hydroxyapatite-deposition-on-polystyrene-plate-surfaces-in-simulated-body-fluids
#20
Kazutoshi Iijima, Ayako Iizuka, Ryo Suzuki, Hitomi Ueno-Yokohata, Nobutaka Kiyokawa, Mineo Hashizume
We have developed a method to functionalize polystyrene (PS) cell culture plates with hydroxyapatite (HAp) via protein adsorption layers such as human serum albumin (HSA) in simulated body fluids (SBFs). In order to investigate the versatility the method, in this study the effect of protein adsorption layers on HAp deposition on PS plate surfaces in SBF was evaluated. Pretreatments with alternate soaking process (ASP) using solutions containing calcium ions and phosphate ions followed by incubation with SBF for 24 h resulted in HAp deposition on PS plates with adsorption layers of HSA, type I collagen, hen egg white lysozyme, and poly L-glutamic acid, an acidic protein analogue: the deposition behaviors were correlated with adsorption ability and charge state of proteins...
November 15, 2017: Journal of Materials Science. Materials in Medicine
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