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https://www.readbyqxmd.com/read/28222834/acute-and-chronic-outcomes-of-gas-bubble-disease-in-a-colony-of-african-clawed-frogs-xenopus-laevis
#1
Julia Y Tsai, Stephen A Felt, Donna M Bouley, Sherril L Green
Gas-bubble disease occurs in aquatic species that are exposed to water that is supersaturated with gases. In February 2007, municipal water supersaturated with gas was inadvertently pumped into the vivarium's aquatic housing systems and affected approximately 450 adult female Xenopus laevis. The inflow of supersaturated water was stopped immediately, the holding tanks aggressively aerated, and all experimental manipulations and feeding ceased. Within the first 6 h after the event, morbidity approached 90%, and mortality reached 3...
February 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28213538/colonization-and-transmission-of-the-gut-microbiota-of-the-burying-beetle-nicrophorus-vespilloides-through-development
#2
Yin Wang, Daniel E Rozen
Carrion beetles in the genus Nicrophorus rear their offspring on decomposing carcasses where larvae are exposed to a diverse community of decomposer bacteria. Parents coat the carcass with antimicrobial secretions prior to egg hatch (defined as Pre-Hatch care) and also feed regurgitated food, and potentially bacteria, to larvae throughout development (defined as Full care). Here we partition the roles of pre- and post-hatch parental care in the transmission and persistence of culturable symbiotic bacteria to larvae...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28199121/smart-contact-lenses-with-graphene-coating-for-electromagnetic-interference-shielding-and-dehydration-protection
#3
Sangkyu Lee, Insu Jo, Sangmin Kang, Bongchul Jang, Joonhee Moon, Jong Bo Park, Soochang Lee, Sichul Rho, Youngsoo Kim, Byung Hee Hong
Recently, smart contact lenses with electronic circuits have been proposed for various sensor and display applications where the use of flexible and biologically stable electrode materials is essential. Graphene is an atomically thin carbon material with a two-dimensional hexagonal lattice that shows outstanding electrical and mechanical properties as well as excellent biocompatibility. In addition, graphene is capable of protecting eyes from electromagnectic (EM) waves that may cause eye diseases such as cataracts...
February 21, 2017: ACS Nano
https://www.readbyqxmd.com/read/28114360/extracellular-ca2-is-required-for-fertilization-in-the-african-clawed-frog-xenopus-laevis
#4
Katherine L Wozniak, Brianna L Mayfield, Alexis M Duray, Maiwase Tembo, David O Beleny, Marc A Napolitano, Monica L Sauer, Bennett W Wisner, Anne E Carlson
BACKGROUND: The necessity of extracellular Ca2+ for fertilization and early embryonic development in the African clawed frog, Xenopus laevis, is controversial. Ca2+ entry into X. laevis sperm is reportedly required for the acrosome reaction, yet fertilization and embryonic development have been documented to occur in high concentrations of the Ca2+ chelator BAPTA. Here we sought to resolve this controversy. METHODOLOGY/PRINCIPAL FINDING: Using the appearance of cleavage furrows as an indicator of embryonic development, we found that X...
2017: PloS One
https://www.readbyqxmd.com/read/28109029/reproductive-performance-of-breeder-quails-fed-diets-supplemented-with-l-cysteine-coated-iron-oxide-nanoparticles
#5
H Mohammadi, A Farzinpour, A Vaziry
The objective of this study was to investigate the effects of L-cysteine-coated iron oxide nanoparticles on reproductive performance in breeder quails. The five treatment diets consisted of (i) negative control diet not supplemented with iron, (ii) positive control diet supplemented with 60 mg/kg of Fe3 O4 and (iii) experimental diets supplemented with 0.6, 6 and 60 mg/kg of L-cysteine-coated iron oxide nanoparticles. A total of 100 seven-day-old quail chicks were weighed and randomly placed to five groups of five replicate cages...
January 20, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28063183/symbiotic-bacteria-associated-with-a-bobtail-squid-reproductive-system-are-detectable-in-the-environment-and-stable-in-the-host-and-in-eggs-throughout-development
#6
Allison H Kerwin, Spencer V Nyholm
Female Hawaiian bobtail squid, Euprymna scolopes, have an accessory nidamental gland (ANG) housing a bacterial consortium that is hypothesized to be environmentally transmitted and to function in the protection of eggs from fouling and infection. The composition, stability, and variability of the ANG and egg jelly coat (JC) communities were characterized and compared to the bacterial community composition of the surrounding environment using Illumina sequencing and transmission electron microscopy. The ANG bacterial community was conserved throughout hosts collected from the wild and was not affected by maintaining animals in the laboratory...
January 7, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28043567/detection-of-mycobacterium-avium-subsp-paratuberculosis-in-cattle-by-using-indirect-absorbed-elisa-enzyme-linked-immunosorbent-assay-system-and-culture-in-alborz-province-iran
#7
Hedyeh Teymouri, Nader Mosavari, Hadi Poor Taghi
OBJECTIVE/BACKGROUND: Mycobacterium avium subsp. paratuberculosis is a slow growing, Gram-positive, and acid-fast bacillus. It is the causative agent of the chronic enteritis disease of ruminants called Johne's disease. Most of the infected animals are young. However, most clinical cases belong to adult cattle aged 3-5years. Due to a prolonged incubation period, identification of subclinically infected animals is one of the most crucial problems in Johne's disease. The aim of this study was to detect M...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28007977/genomic-basis-of-adaptive-evolution-the-survival-of-amur-ide-leuciscus-waleckii-in-an-extremely-alkaline-environment
#8
Jian Xu, Jiong-Tang Li, Yanliang Jiang, Wenzhu Peng, Zongli Yao, Baohua Chen, Likun Jiang, Jingyan Feng, Peifeng Ji, Guiming Liu, Zhanjiang Liu, Ruyu Tai, Chuanju Dong, Xiaoqing Sun, Zi-Xia Zhao, Yan Zhang, Jian Wang, Shangqi Li, Yunfeng Zhao, Jiuhui Yang, Xiaowen Sun, Peng Xu
The Amur ide (Leuciscus waleckii) is a cyprinid fish that is widely distributed in Northeast Asia. The Lake Dali Nur population inhabits one of the most extreme aquatic environments on Earth, with an alkalinity up to 50 mmol/L (pH 9.6), thus providing an exceptional model with which to characterize the mechanisms of genomic evolution underlying adaptation to extreme environments. Here, we developed the reference genome assembly for L. waleckii from Lake Dali Nur. Intriguingly, we identified unusual expanded long terminal repeats (LTRs) with higher nucleotide substitution rates than in many other teleosts, suggesting their more recent insertion into the L...
October 20, 2016: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28002933/effects-of-different-methionine-sources-on-production-and-reproduction-performance-egg-quality-and-serum-biochemical-indices-of-broiler-breeders
#9
Xue Xiao, Yongxia Wang, Weilong Liu, Tingting Ju, Xiuan Zhan
The study was conducted to evaluate the effects of different methionine (Met) sources on production performance, reproduction performance, egg quality and serum biochemical indices in broiler breeders. After receiving a basal diet (containing 0.25% Met) for a 2-wk pretreatment period, a total of 360 39-wk-old Lingnan yellow broiler breeders were randomly allocated to four treatments with six replicates each (15 birds per replicate). Breeders were fed with basal diets (control) or diets supplemented with DL-methionine (DLM), DL-2-hydroxy-4-methylthio butytric calcium (MHA-Ca) and coated DL-Met (CME) respectively (containing 0...
December 17, 2016: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/27982574/an-environmentally-benign-antimicrobial-coating-based-on-a-protein-supramolecular-assembly
#10
Jin Gu, Yajuan Su, Peng Liu, Peng Li, Peng Yang
The use of antimicrobial materials, for example, silver nanoparticles, has been a cause for concern because they often exert an adverse effect on environmental and safety during their preparation and use. In this study, we report a class of green antimicrobial coating based on a supramolecular assembly of a protein extracted from daily food, without the addition of any other hazardous agents. It is found that a self-assembled nanofilm by mere hen egg white lysozyme has durable in vitro and in vivo broad-spectrum antimicrobial efficacy against Gram-positive/negative and fungi...
December 23, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27976499/imidacloprid-seed-treatments-affect-individual-ant-behavior-and-community-structure-but-not-egg-predation-pest-abundance-or-soybean-yield
#11
Hannah J Penn, Andrew M Dale
BACKGROUND: Neonicotinoid seed treatments are under scrutiny because of their variable efficacy against crop pests and for their potential negative impacts on non-target organisms. Ants provide important biocontrol services in agroecosystems and can be indicators of ecosystem health. This study tested for effects of exposure to imidacloprid plus fungicide or fungicide treated seeds on individual ant survival, locomotion and foraging capabilities, and on field ant community structure, pest abundance, ant predation, and yield...
December 15, 2016: Pest Management Science
https://www.readbyqxmd.com/read/27886653/waffle-production-influence-of-baking-plate-material-on-sticking-of-waffles
#12
Regina Huber, Georg Kalss, Regine Schoenlechner
Background of this study was to understand the factors that contribute to sticking of fresh egg waffles on baking plates. The aim of this study was to investigate the sticking (adhesion) behavior of waffles on 4 different baking plate materials (ductile iron, grey iron, low alloyed steel, and steel with titanium nitrite coating) at different baking parameters (temperature and time) and application of 3 different release agents (different fat compositions). Baking plates from ductile and grey iron showed lower release properties of waffles than the 2 steel baking plates...
January 2017: Journal of Food Science
https://www.readbyqxmd.com/read/27855494/chitosan-coated-plga-nanoparticles-of-bevacizumab-as-novel-drug-delivery-to-target-retina-optimization-characterization-and-in-vitro-toxicity-evaluation
#13
Jayamanti Pandit, Yasmin Sultana, Mohd Aqil
In several ocular diseases, the vascular endothelial growth factor (VEGF) level has been found to be upregulated. Bevacizumab, an anti-VEGF drug, is the most commonly used off level drug for these conditions. Delivery of drug to the posterior site is desired for the effective management of these diseases. The present study was to develop and optimize the chitosan (CS)-coated poly(lactide-co-glycolic acid) (PLGA) nanoparticles (NPs) of bevacizumab for sustained and effective delivery to posterior ocular tissues...
November 18, 2016: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/27853913/differences-in-pregnancy-outcomes-in-donor-egg-frozen-embryo-transfer-fet-cycles-following-preimplantation-genetic-screening-pgs-a-single-center-retrospective-study
#14
Alison Coates, Brandon J Bankowski, Allen Kung, Darren K Griffin, Santiago Munne
PURPOSE: This study aims to test the hypothesis, in a single-center retrospective analysis, that live birth rates are significantly different when utilizing preimplantation genetic screening (PGS) compared to not utilizing PGS in frozen-thawed embryo transfers in our patients that use eggs from young, anonymous donors. The question therefore arises of whether PGS is an appropriate intervention for donor egg cycles. METHODS: Live birth rates per cycle and live birth rates per embryo transferred after 398 frozen embryo transfer (FET) cycles were examined from patients who elected to have PGS compared to those who did not...
November 16, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27848912/auxin-production-in-the-endosperm-drives-seed-coat-development-in-arabidopsis
#15
Duarte D Figueiredo, Rita A Batista, Pawel J Roszak, Lars Hennig, Claudia Köhler
In flowering plants, seed development is initiated by the fusion of the maternal egg and central cells with two paternal sperm cells, leading to the formation of embryo and endosperm, respectively. The fertilization products are surrounded by the maternally derived seed coat, whose development prior to fertilization is blocked by epigenetic regulators belonging to the Polycomb Group (PcG) protein family. Here we show that fertilization of the central cell results in the production of auxin and most likely its export to the maternal tissues, which drives seed coat development by removing PcG function...
November 16, 2016: ELife
https://www.readbyqxmd.com/read/27819041/pollen-tube-contents-initiate-ovule-enlargement-and-enhance-seed-coat-development-without-fertilization
#16
Ryushiro D Kasahara, Michitaka Notaguchi, Shiori Nagahara, Takamasa Suzuki, Daichi Susaki, Yujiro Honma, Daisuke Maruyama, Tetsuya Higashiyama
In angiosperms, pollen tubes carry two sperm cells toward the egg and central cells to complete double fertilization. In animals, not only sperm but also seminal plasma is required for proper fertilization. However, little is known regarding the function of pollen tube content (PTC), which is analogous to seminal plasma. We report that the PTC plays a vital role in the prefertilization state and causes an enlargement of ovules without fertilization. We termed this phenomenon as pollen tube-dependent ovule enlargement morphology and placed it between pollen tube guidance and double fertilization...
October 2016: Science Advances
https://www.readbyqxmd.com/read/27709298/removal-of-extracellular-coat-from-giant-sperm-in-female-receptacle-induces-sperm-motility-in-mytilocypris-mytiloides-cyprididae-ostracoda-crustacea
#17
Renate Matzke-Karasz, Robin J Smith, Martin Heß
Previous studies of cypridoidean ostracods have noted that (1) their giant spermatozoa are immotile inside the male, (2) these spermatozoa are motile in the female seminal receptacle and (3) these receptacles are often filled with empty sperm coats. Such findings have led previous authors to hypothesize that sperm must shed their coats in the female receptacle to become motile. We present light and electron microscopy results and video recordings of mating experiments with virgin specimens of Mytilocypris mytiloides...
October 5, 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27660622/leisingera-sp-jc1-a-bacterial-isolate-from-hawaiian-bobtail-squid-eggs-produces-indigoidine-and-differentially-inhibits-vibrios
#18
Samantha M Gromek, Andrea M Suria, Matthew S Fullmer, Jillian L Garcia, Johann Peter Gogarten, Spencer V Nyholm, Marcy J Balunas
Female members of many cephalopod species house a bacterial consortium in the accessory nidamental gland (ANG), part of the reproductive system. These bacteria are deposited into eggs that are then laid in the environment where they must develop unprotected from predation, pathogens, and fouling. In this study, we characterized the genome and secondary metabolite production of Leisingera sp. JC1, a member of the roseobacter clade (Rhodobacteraceae) of Alphaproteobacteria isolated from the jelly coat of eggs from the Hawaiian bobtail squid, Euprymna scolopes...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27622321/coated-protein-nanoclusters-from-influenza-h7n9-ha-are-highly-immunogenic-and-induce-robust-protective-immunity
#19
Li Wang, Timothy Z Chang, Yuan He, Jong R Kim, Shelly Wang, Teena Mohan, Zachary Berman, S Mark Tompkins, Ralph A Tripp, Richard W Compans, Julie A Champion, Bao-Zhong Wang
Recurring influenza viruses pose an annual threat to public health. A time-saving, cost-effective and egg-independent influenza vaccine approach is important particularly when responding to an emerging pandemic. We fabricated coated, two-layer protein nanoclusters from recombinant trimeric hemagglutinin from an avian-origin H7N9 influenza A virus as an approach for vaccine development in response to an emerging pandemic. Assessment of the virus-specific immune responses and protective efficacy in mice immunized with the nanoclusters demonstrated that the vaccine candidates were highly immunogenic, able to induce protective immunity and long-lasting humoral antibody responses to this virus without the use of adjuvants...
January 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27602804/gels-prepared-from-egg-yolk-and-its-fractions-for-tissue-engineering
#20
Andrea Rodil, Amanda Laca, Benjamín Paredes, Manuel Rendueles, Álvaro Meana, Mario Díaz
New biomaterials prepared from egg yolk and its main fractions (plasma and granules) have been developed for use in tissue engineering. Protein gels obtained via transglutaminase cross-linking were characterized by rheometry, texturometry and scanning electron microscopy. All the gels exhibited suitable physical and mechanical characteristics for use as potential biomaterials in skin regeneration. Specifically, results showed that these materials presented a compact, uniform structure, with granular gel being found to be the most resistant as well as the most elastic material...
November 2016: Biotechnology Progress
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