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https://www.readbyqxmd.com/read/27886653/waffle-production-influence-of-baking-plate-material-on-sticking-of-waffles
#1
Regina Huber, Georg Kalss, Regine Schoenlechner
Background of this study was to understand the factors that contribute to sticking of fresh egg waffles on baking plates. The aim of this study was to investigate the sticking (adhesion) behavior of waffles on 4 different baking plate materials (ductile iron, grey iron, low alloyed steel, and steel with titanium nitrite coating) at different baking parameters (temperature and time) and application of 3 different release agents (different fat compositions). Baking plates from ductile and grey iron showed lower release properties of waffles than the 2 steel baking plates...
November 25, 2016: Journal of Food Science
https://www.readbyqxmd.com/read/27855494/chitosan-coated-plga-nanoparticles-of-bevacizumab-as-novel-drug-delivery-to-target-retina-optimization-characterization-and-in-vitro-toxicity-evaluation
#2
Jayamanti Pandit, Yasmin Sultana, Mohd Aqil
In several ocular diseases, the vascular endothelial growth factor (VEGF) level has been found to be upregulated. Bevacizumab, an anti-VEGF drug, is the most commonly used off level drug for these conditions. Delivery of drug to the posterior site is desired for the effective management of these diseases. The present study was to develop and optimize the chitosan (CS)-coated poly(lactide-co-glycolic acid) (PLGA) nanoparticles (NPs) of bevacizumab for sustained and effective delivery to posterior ocular tissues...
November 18, 2016: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/27853913/differences-in-pregnancy-outcomes-in-donor-egg-frozen-embryo-transfer-fet-cycles-following-preimplantation-genetic-screening-pgs-a-single-center-retrospective-study
#3
Alison Coates, Brandon J Bankowski, Allen Kung, Darren K Griffin, Santiago Munne
PURPOSE: This study aims to test the hypothesis, in a single-center retrospective analysis, that live birth rates are significantly different when utilizing preimplantation genetic screening (PGS) compared to not utilizing PGS in frozen-thawed embryo transfers in our patients that use eggs from young, anonymous donors. The question therefore arises of whether PGS is an appropriate intervention for donor egg cycles. METHODS: Live birth rates per cycle and live birth rates per embryo transferred after 398 frozen embryo transfer (FET) cycles were examined from patients who elected to have PGS compared to those who did not...
November 16, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27848912/auxin-production-in-the-endosperm-drives-seed-coat-development-in-arabidopsis
#4
Duarte D Figueiredo, Rita A Batista, Pawel J Roszak, Lars Hennig, Claudia Köhler
In flowering plants, seed development is initiated by the fusion of the maternal egg and central cells with two paternal sperm cells, leading to the formation of embryo and endosperm, respectively. The fertilization products are surrounded by the maternally derived seed coat, whose development prior to fertilization is blocked by epigenetic regulators belonging to the Polycomb Group (PcG) protein family. Here we show that fertilization of the central cell results in the production of auxin and most likely its export to the maternal tissues, which drives seed coat development by removing PcG function...
November 16, 2016: ELife
https://www.readbyqxmd.com/read/27819041/pollen-tube-contents-initiate-ovule-enlargement-and-enhance-seed-coat-development-without-fertilization
#5
Ryushiro D Kasahara, Michitaka Notaguchi, Shiori Nagahara, Takamasa Suzuki, Daichi Susaki, Yujiro Honma, Daisuke Maruyama, Tetsuya Higashiyama
In angiosperms, pollen tubes carry two sperm cells toward the egg and central cells to complete double fertilization. In animals, not only sperm but also seminal plasma is required for proper fertilization. However, little is known regarding the function of pollen tube content (PTC), which is analogous to seminal plasma. We report that the PTC plays a vital role in the prefertilization state and causes an enlargement of ovules without fertilization. We termed this phenomenon as pollen tube-dependent ovule enlargement morphology and placed it between pollen tube guidance and double fertilization...
October 2016: Science Advances
https://www.readbyqxmd.com/read/27709298/removal-of-extracellular-coat-from-giant-sperm-in-female-receptacle-induces-sperm-motility-in-mytilocypris-mytiloides-cyprididae-ostracoda-crustacea
#6
Renate Matzke-Karasz, Robin J Smith, Martin Heß
Previous studies of cypridoidean ostracods have noted that (1) their giant spermatozoa are immotile inside the male, (2) these spermatozoa are motile in the female seminal receptacle and (3) these receptacles are often filled with empty sperm coats. Such findings have led previous authors to hypothesize that sperm must shed their coats in the female receptacle to become motile. We present light and electron microscopy results and video recordings of mating experiments with virgin specimens of Mytilocypris mytiloides...
October 5, 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27660622/leisingera-sp-jc1-a-bacterial-isolate-from-hawaiian-bobtail-squid-eggs-produces-indigoidine-and-differentially-inhibits-vibrios
#7
Samantha M Gromek, Andrea M Suria, Matthew S Fullmer, Jillian L Garcia, Johann Peter Gogarten, Spencer V Nyholm, Marcy J Balunas
Female members of many cephalopod species house a bacterial consortium in the accessory nidamental gland (ANG), part of the reproductive system. These bacteria are deposited into eggs that are then laid in the environment where they must develop unprotected from predation, pathogens, and fouling. In this study, we characterized the genome and secondary metabolite production of Leisingera sp. JC1, a member of the roseobacter clade (Rhodobacteraceae) of Alphaproteobacteria isolated from the jelly coat of eggs from the Hawaiian bobtail squid, Euprymna scolopes...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27622321/coated-protein-nanoclusters-from-influenza-h7n9-ha-are-highly-immunogenic-and-induce-robust-protective-immunity
#8
Li Wang, Timothy Z Chang, Yuan He, Jong R Kim, Shelly Wang, Teena Mohan, Zachary Berman, S Mark Tompkins, Ralph A Tripp, Richard W Compans, Julie A Champion, Bao-Zhong Wang
Recurring influenza viruses pose an annual threat to public health. A time-saving, cost-effective and egg-independent influenza vaccine approach is important particularly when responding to an emerging pandemic. We fabricated coated, two-layer protein nanoclusters from recombinant trimeric hemagglutinin from an avian-origin H7N9 influenza A virus as an approach for vaccine development in response to an emerging pandemic. Assessment of the virus-specific immune responses and protective efficacy in mice immunized with the nanoclusters demonstrated that the vaccine candidates were highly immunogenic, able to induce protective immunity and long-lasting humoral antibody responses to this virus without the use of adjuvants...
September 10, 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27602804/gels-prepared-from-egg-yolk-and-its-fractions-for-tissue-engineering
#9
Andrea Rodil, Amanda Laca, Benjamín Paredes, Manuel Rendueles, Álvaro Meana, Mario Díaz
New biomaterials prepared from egg yolk and its main fractions (plasma and granules) have been developed for use in tissue engineering. Protein gels obtained via transglutaminase cross-linking were characterized by rheometry, texturometry and scanning electron microscopy. All the gels exhibited suitable physical and mechanical characteristics for use as potential biomaterials in skin regeneration. Specifically, results showed that these materials presented a compact, uniform structure, with granular gel being found to be the most resistant as well as the most elastic material...
September 7, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27591546/sugarcoated-sperm
#10
Jean-Ju Chung
The'sugarcoat', a dense carbohydrate coat (glycocalyx), is critical to cell-to-cell recognition, including sperm-egg interactions. Lectins are used to probe the localization of glycoproteins on the cell surface, and to track their changes during sperm maturation and capacitation (Tecle and Gagneux, 2015, Mol. Reprod. Dev 82:635-650). Among them, wheat germ agglutinin (WGA) binds to the sperm head, cytoplasmic droplet, midpiece, as well as the principal piece and end piece of the sperm tail (Fabrega et al, 2012, Reprod...
September 3, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27581379/characterization-of-a-liposome-based-artificial-skin-membrane-for-in-vitro-permeation-studies-using-franz-diffusion-cell-device
#11
Hui Zhang, Xuemin Zhu, Jingjing Shen, Haiheng Xu, Min Ma, Wei Gu, Qiudong Jiang, Jun Chen, Jinao Duan
A prerequisite for successful transdermal or dermal drug therapy is the drug ability to penetration through the skin, especially stratum corneum (SC). The most acceptable technique for measuring skin permeation in vitro is the application of both the Franz diffusion cell device and the skin model. As the skin model, a liposome-based artificial skin membrane (LASM) consisting of tight layers of liposomes immobilized on a filter was prepared and characterized. Using porcine ear skin, rat skin and Strat-M(TM) artificial membrane as control, the LASM was then evaluated in permeation studies with five active compounds, i...
August 31, 2016: Journal of Liposome Research
https://www.readbyqxmd.com/read/27579691/a-novel-cysteine-knot-protein-for-enhancing-sperm-motility-that-might-facilitate-the-evolution-of-internal-fertilization-in-amphibians
#12
Misato Yokoe, Eriko Takayama-Watanabe, Yoko Saito, Megumi Kutsuzawa, Kosuke Fujita, Haruki Ochi, Yuni Nakauchi, Akihiko Watanabe
Internal fertilization ensures successful reproduction of tetrapod vertebrates on land, although how this mode of reproduction evolved is unknown. Here, we identified a novel gene encoding sperm motility-initiating substance (SMIS), a key protein for the internal fertilization of the urodele Cynops pyrrhogaster by Edman degradation of an isolated protein and subsequent reverse transcription polymerase chain reaction. The SMIS gene encoded a 150 amino-acid sequence including the cysteine knot (CK) motif. No gene with substantial similarity to the SMIS was in the data bank of any model organisms...
2016: PloS One
https://www.readbyqxmd.com/read/27555440/polydopamine-assisted-fabrication-of-titanium-oxide-nanoparticles-modified-column-for-proteins-separation-by-capillary-electrochromatography
#13
Yamin Zhang, Wentao Wang, Xiangdong Ma, Li Jia
Development of a simple method for preparation of stable open tubular (OT) columns for proteins separation by capillary electrochromatography is still challenging. In this work, the titanium oxide (TiO2) nanoparticles coated OT column was successfully prepared for separation of proteins by capillary electrochromatography. The polydopamine (PDA) film was first formed in the inner surface of a fused-silica capillary by the self-polymerization of dopamine under alkaline conditions. Then the TiO2 coating was deposited onto the surface of pre-modified capillary with PDA by a liquid phase deposition process...
November 1, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/27534384/salinity-dependent-toxicity-of-water-dispersible-single-walled-carbon-nanotubes-to-japanese-medaka-embryos
#14
Chisato Kataoka, Kousuke Nakahara, Kaori Shimizu, Shinsuke Kowase, Seiji Nagasaka, Shinsuke Ifuku, Shosaku Kashiwada
To investigate the effects of salinity on the behavior and toxicity of functionalized single-walled carbon nanotubes (SWCNTs), which are chemical modified nanotube to increase dispersibility, medaka embryos were exposed to non-functionalized single-walled carbon nanotubes (N-SWCNTs), water-dispersible, cationic, plastic-polymer-coated, single-walled carbon nanotubes (W-SWCNTs), or hydrophobic polyethylene glycol-functionalized, single-walled carbon nanotubes (PEG-SWCNTs) at different salinities, from freshwater to seawater...
August 18, 2016: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/27499207/vascular-toxicity-of-silver-nanoparticles-to-developing-zebrafish-danio-rerio
#15
Jiejun Gao, Cecon T Mahapatra, Christopher D Mapes, Maria Khlebnikova, Alexander Wei, Marisol S Sepúlveda
Nanoparticles (NPs, 1-100 nm) can enter the environment and result in exposure to humans and other organisms leading to potential adverse health effects. The aim of the present study is to evaluate the effects of early life exposure to polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs, 50 nm), particularly with respect to vascular toxicity on zebrafish embryos and larvae (Danio rerio). Previously published data has suggested that PVP-AgNP exposure can inhibit the expression of genes within the vascular endothelial growth factor (VEGF) signaling pathway, leading to delayed and abnormal vascular development...
November 2016: Nanotoxicology
https://www.readbyqxmd.com/read/27433575/clozapine-loaded-polysorbate-coated-polymeric-nanocapsules-physico-chemical-characterization-and-toxicity-evaluation-in-caenorhabditis-elegans-model
#16
BarbraKatyúscya Sanches Moraes, Simone Machado Vieira, Willian Goulart Salgueiro, Luana Roberta Michels, Letícia Marques Colomé, Daiana Silva Avila, Sandra Elisa Haas
The aim of this work was to develop and characterize clozapine loaded polysorbate-coated polymeric nanocapsules and assess their toxicity in Caenorhabditis elegans, an invertebrate animal model. Formulations were prepared by nanoprecipitation method and characterized by particle size, zeta potential, pH, drug loading, entrapment efficiency and in vitro drug release. All nanocapsules prepared presented diameter around 140 nm, pH slightly acid and negative zeta potential. In vitro studies showed biphasic drug release from nanocapsules with decreasing of the release rate on nanoencapsulation...
February 2016: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/27371913/liposomal-nanoformulations-of-rhodamine-for-targeted-photodynamic-inactivation-of-multidrug-resistant-gram-negative-bacteria-in-sewage-treatment-plant
#17
Mohan Vimaladevi, Kurunchi Chellapathi Divya, Agnishwar Girigoswami
The antimicrobial photodynamic therapy is an alternative method for killing bacterial cells in view of the rising problem of antibiotic resistance microorganisms. The present study examined the effect of a water soluble photosensitizer, Rhodamine 6G (R6G) in stealth liposomes on multidrug resistant Pseudomonas aeruginosa in the presence of visible light. Liposomes were prepared with cholesterol and phospholipids that extracted from hen eggs in a cost effective way and characterized by light microscopy, particle size analyzer, electron microscopy, steady state spectrophotometry and spectrofluorometry...
September 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27333873/embryology-of-cardiopteris-cardiopteridaceae-aquifoliales-with-emphasis-on-unusual-ovule-and-seed-development
#18
Hiroshi Tobe
Cardiopteris (Cardiopteridaceae), a twining herb of two or three species distributed from Southeast Asia to Northern Australia, requires an embryological study for better understanding of its reproductive features. The present study of C. quinqueloba showed that the ovule and seed development involves a number of unusual structures, most of which are unknown elsewhere in angiosperms. The ovule pendant from the apical placenta is straight (not orthotropous), ategmic, and tenuinucellate, developing a monosporic seven-celled/eight-nucleate female gametophyte with an egg apparatus on the funicular side...
September 2016: Journal of Plant Research
https://www.readbyqxmd.com/read/27282368/effects-of-%C3%AE-fe2o3-nanoparticles-on-the-survival-and-reproduction-of-biomphalaria-glabrata-say-1818-and-their-elimination-from-this-benthic-aquatic-snail
#19
Eduardo C Oliveira-Filho, José Sousa Filho, Luana A Novais, Wilson S Peternele, Ricardo B Azevedo, Cesar K Grisolia
This study aims to evaluate the effects of maghemite nanoparticles (γ-Fe2O3) coated with meso-2, 3-dimercaptosuccinic acid (DMSA) stabilizer on the survival and reproduction of the aquatic snail Biomphalaria glabrata. The cumulative means of egg masses and eggs per individual in the control group at the end of 4 weeks were 18.8 and 326.7, respectively. These values at the concentration of 1 mg/L were 17.2 and 291.6; at 10 mg/L, they were 19.6 and 334.4 ,and at 100 mg/L, they were 14.3 and 311.1. Results showed no significant differences between the tested and the control groups at the level of p < 0...
September 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27252373/inactivation-of-salmonella-enteritidis-on-shell-eggs-by-coating-with-phytochemicals
#20
Indu Upadhyaya, H-B Yin, M Surendran Nair, C-H Chen, R Lang, M J Darre, K Venkitanarayanan
Salmonella enteritidis (SE) is a major foodborne pathogen that causes human infections largely by consumption of contaminated eggs. The external surface of eggs becomes contaminated with SE from multiple sources, highlighting the need for effective egg surface disinfection methods. This study investigated the efficacy of three GRAS-status, phytochemicals, namely carvacrol (CR), eugenol (EG), and β-resorcylic acid (BR) applied as pectin or gum arabic based coating for reducing SE on shell eggs. White-shelled eggs, spot inoculated with a 5-strain mixture of nalidixic acid (NA) resistant SE (8...
September 1, 2016: Poultry Science
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