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egg coating

Swati Jagdale, Gokul Deore, Anuruddha Chabukswar
Background Nabumetone is biopharmaceutics classification system (BCS) class II drug, widely used in the treatment of osteoarthritis and rheumatoid arthritis. The most frequently reported adverse reactions for the drug involve disturbance in gastrointestinal tract , diarrhea, dyspepsia and abdominal pain. Microemulgel has advantages of microemulsion for improving solubility for hydrophobic drug. Patent literature had shown that the work for drug has been carried on spray chilling, enteric coated tablet, and topical formulation which gave idea for present research work for development of transdermal delivery...
February 26, 2018: Recent Patents on Drug Delivery & Formulation
Dae-Won Lee, Hira Fatima, Kyo-Seon Kim
Magnetic nanoparticles have been intensively developed and applied in several biomedical applications such as targeted delivery, drug therapy, hyperthermia, magnetic resonance imaging and bioseparation etc. This work describes a simple and convenient method to synthesize silica coated Fe3O4 nanoparticles which have the potential to be used in the bioseparation of fetuin from fetal bovine serum and albumin from the egg. In this work, uniform Fe3O4 particles were prepared through the one-pot solvothermal process at 200 °C for 12 h using a sole iron precursor (FeCl3 · 6H2O) and then were coated with SiO2 to prepare silica coated Fe3O4 nanoparticles by hydrolysis and condensation of tetraethyl orthosilicate in ethanol and H2O medium...
February 1, 2018: Journal of Nanoscience and Nanotechnology
Qiang Ma, Yuxian Wang, Jie Jia, Yuhong Xiang
Colorimetric aptasensors were designed for detection of tobramycin (TOB) based on unmodified gold nanoparticles (AuNPs) and single-strand DNA (ssDNA). In the absence of TOB, the DNA aptamer was coated on the surface of AuNPs to keep it against salt-induced aggregation. In the presence of TOB, aptamer will bind with TOB and detach from the surface of AuNPs because of higher affinities between aptamer and TOB. Then less protection of DNA may result in the aggregation of AuNPs by salt and an apparent color change from red to purple-blue...
May 30, 2018: Food Chemistry
Luping Gu, Che Pan, Yujie Su, Ruojie Zhang, Hang Xiao, David Julian McClements, Yan-Jun Yang
Previously, it was shown that catechin-egg white protein (CT-EWP) conjugates were effective antioxidant emulsifiers that could form and stabilize emulsions, and also inhibit the degradation of encapsulated carotenoids. The objective of the current study was to evaluate the impact of conjugation on the in vitro bioavailability, cellular antioxidant activity, and cytotoxicity of β-carotene-loaded emulsions. Lipid droplets coated with EWP or with CT-EWP conjugates exhibited quite similar behavior when they were passed through a simulated gastrointestinal tract...
January 31, 2018: Journal of Agricultural and Food Chemistry
Michael W Hart, Daryn A Stover, Vanessa Guerra, Sahar V Mozaffari, Carole Ober, Carina F Mugal, Ingemar Kaj
Coevolution of genes that encode interacting proteins expressed on the surfaces of sperm and eggs can lead to variation in reproductive compatibility between mates and reproductive isolation between members of different species. Previous studies in mice and other mammals have focused in particular on evidence for positive or diversifying selection that shapes the evolution of genes that encode sperm-binding proteins expressed in the egg coat or zona pellucida (ZP). By fitting phylogenetic models of codon evolution to data from the 1000 Genomes Project, we identified candidate sites evolving under diversifying selection in the human genes ZP3 and ZP2...
2018: PeerJ
Tianli Wu, Yunying Cheng, Zhilong Liu, Wenjing Tao, Shuqing Zheng, Deshou Wang
Zona pellucida (ZP) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and other vertebrates. Totally 16, 9, 17, 27, 21, 20, 26, 19, 14,11, 24, 17, 9, 18, 8, 11, 9, 8, 5, and 4 ZP genes belonging to 5 subfamilies (ZPA, ZPB, ZPC, ZPD, and ZPAX) were found in the sea lamprey, elephant shark, coelacanth, spotted gar, zebrafish, medaka, stickleback, Nile tilapia, Amazon molly, platyfish, seahorse, Northern snakehead, cavefish, tetraodon, clawed frog, turtle, chicken, platypus, kangaroo rat, and human genomes, respectively...
January 6, 2018: Fish Physiology and Biochemistry
Ying Bian, Changshan Liu, Tiantian She, Mengjuan Wang, Juanjuan Yan, Dianjun Wei, Huiqiang Li
The determination of specific IgE (sIgE) level is of great importance in IgE-mediated food allergies. Our aim was to develop a homogeneous immunoassay-light-initiated chemiluminescent assay (LICA)-for measuring allergen sIgE of a single component in egg white, thus evaluating the LICA-sIgE assay as a useful tool in the diagnosis of food allergy. The LICA-sIgE assay was performed by incubating serum sample with anti-human IgE antibody coated with chemiluminescer beads, streptavidin-coated sensitizer beads, and biotinylated antigens, which consist of four components in egg white...
December 15, 2017: Analytical and Bioanalytical Chemistry
Jun Sun, Bin Xu, Yaoyao Mu, Haile Ma, Wenjuan Qu
In this study, magnetic nanoparticles functionalized with carboxylated β-cyclodextrin (CM-β-CD; referred to Fe3 O4 @CM-β-CD) were synthesized and used for the efficient removal of cholesterol from milk and egg yolk via host-guest interactions. The results of Fourier-transform infrared, X-ray photoelectron spectroscopy, and thermogravimetric analysis indicated that the CM-β-CD was successfully conjugated to the surface of Fe3 O4 , and the amount of CM-β-CD attached on Fe3 O4 @CM-β-CD was determined to be 9...
January 2018: Journal of Food Science
Tatiana A Makarikova
A previously unrecognised species of hymenolepidid cestode attributable to Vampirolepis Spassky, 1954 is described based on specimens from the common noctule bat Nyctalus noctula (Schreber) (Chiroptera: Vespertilionidae) from southeastern Kazakhstan (Dzungarian Alatau). Specimens of Vampirolepis kulkinae n. sp. differ from the morphologically similar congeners based on the number, size and shape of the rostellar hooks. The new species is further distinguished from additional cestodes attributed to Vampirolepis (sensu lato) by the arrangement of the testes in one row, egg structure (i...
January 2018: Systematic Parasitology
Yaqun Zou, Liang Zhao, Guangxin Feng, Yu Miao, Haohao Wu, Mingyong Zeng
Anchovy (Engraulis japonicus) meat (AM) has been shown to promote nonheme iron absorption via a ferric oxyhydroxide nanoparticle (FeONP)-mediated mechanism. Here, formulation modifications of an egg-white-based AIN-93G diet with AM fractions resulted hemoglobin regeneration efficiencies in anemic rats following an order control (23.69 ± 3.99%) < ferrous-sulfate-replacement of ferric citrate (39.89 ± 2.97%) ≈ dehemeed-AM-protein-replacement of egg white (45.88 ± 4.76%) ≈ AM-lipid-replacement of soybean oil (43...
December 27, 2017: Journal of Agricultural and Food Chemistry
P A Nguyen, C M Field, T J Mitchison
The cleavage furrow in Xenopus zygotes is positioned by two large microtubule asters that grow out from the poles of the first mitotic spindle. Where these asters meet at the midplane, they assemble a disk-shaped interaction zone consisting of anti-parallel microtubule bundles coated with chromosome passenger complex (CPC) and centralspindlin that instructs the cleavage furrow. Here we investigate the mechanism that keeps the two asters separate and forms a distinct boundary between them, focusing on the conserved cytokinesis midzone proteins Prc1 and Kif4A...
February 1, 2018: Molecular Biology of the Cell
Kazutoshi Iijima, Ayako Iizuka, Ryo Suzuki, Hitomi Ueno-Yokohata, Nobutaka Kiyokawa, Mineo Hashizume
We have developed a method to functionalize polystyrene (PS) cell culture plates with hydroxyapatite (HAp) via protein adsorption layers such as human serum albumin (HSA) in simulated body fluids (SBFs). In order to investigate the versatility the method, in this study the effect of protein adsorption layers on HAp deposition on PS plate surfaces in SBF was evaluated. Pretreatments with alternate soaking process (ASP) using solutions containing calcium ions and phosphate ions followed by incubation with SBF for 24 h resulted in HAp deposition on PS plates with adsorption layers of HSA, type I collagen, hen egg white lysozyme, and poly L-glutamic acid, an acidic protein analogue: the deposition behaviors were correlated with adsorption ability and charge state of proteins...
November 15, 2017: Journal of Materials Science. Materials in Medicine
Soad Nady, Gamal Esmat
The aim of this study was to investigate the role of Th17 cytokines on granulocytes recruitment and functional activity in Schistosoma mansoni/hepatitis C virus (HCV) co-infected patients. Granulocytes were isolated from the co-infected blood and stimulated overnight with Schistosoma soluble egg antigen (SEA) in the presence of IL-17, IL-22, or both cytokines. In parallel, granuloma was induced in vitro using the isolated granulocytes and SEA-coated polyacrylamide beads in the presence of IL-17 and/or IL-22 then the sizes of granulomas were measured after one week...
January 2017: Egyptian Journal of Immunology
C Jayanthy, B Nagarajan, Bhaskaran Ravi Latha
A Persian cat with the complaint of lustreless dry coat, salt and pepper appearance was brought to the dermatology unit of Madras Veterinary College, Chennai. Microscopical examination of the tape impression and hair coat brushings revealed Lynxacarus radovskyi mites with eggs adhering to the hair shaft. The cat was treated with Ivermectin @ 300 µg/kg body weight subcutaneously once a week for 5 weeks after getting owner's consent. The cat was free from the parasites 5 weeks post treatment. Fomite and close contact might aid the spread of infestation...
December 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
Shikuan Yang, Nan Sun, Birgitt Boschitsch Stogin, Jing Wang, Yu Huang, Tak-Sing Wong
Since the early discovery of the antireflection properties of insect compound eyes, new examples of natural antireflective coatings have been rare. Here, we report the fabrication and optical characterization of a biologically inspired antireflective surface that emulates the intricate surface architectures of leafhopper-produced brochosomes-soccer ball-like microscale granules with nanoscale indentations. Our method utilizes double-layer colloidal crystal templates in conjunction with site-specific electrochemical growth to create these structures, and is compatible with various materials including metals, metal oxides, and conductive polymers...
November 3, 2017: Nature Communications
Niyo Kato, Yoshitaka Kawasoe, Hannah Williams, Elena Coates, Upasana Roy, Yuqian Shi, Lorena S Beese, Orlando D Schärer, Hong Yan, Max E Gottesman, Tatsuro S Takahashi, Jean Gautier
DNA interstrand crosslinks (ICLs) that are repaired in non-dividing cells must be recognized independently of replication-associated DNA unwinding. Using cell-free extracts from Xenopus eggs that support neither replication nor transcription, we establish that ICLs are recognized and processed by the mismatch repair (MMR) machinery. We find that ICL repair requires MutSα (MSH2-MSH6) and the mismatch recognition FXE motif in MSH6, strongly suggesting that MutSα functions as an ICL sensor. MutSα recruits MutLα and EXO1 to ICL lesions, and the catalytic activity of both these nucleases is essential for ICL repair...
October 31, 2017: Cell Reports
Hiroki Okumura
An ovulated egg of vertebrates is surrounded by unique extracellular matrix, the egg coat or zona pellucida, playing important roles in fertilization and early development. The vertebrate egg coat is composed of two to six zona pellucida (ZP) glycoproteins that are characterized by the evolutionarily conserved ZP-domain module and classified into six subfamilies based on phylogenetic analyses. Interestingly, investigations of biochemical and functional features of the ZP glycoproteins show that the roles of each ZP-glycoprotein family member in the egg-coat formation and the egg-sperm interactions seemingly vary across vertebrates...
2017: Advances in Experimental Medicine and Biology
Wen Bo Liao, Yan Huang, Yu Zeng, Mao Jun Zhong, Yi Luo, Stefan Lüpold
The evolution of sperm quality and quantity is shaped by various selective processes, with sperm competition generally considered the primary selective agent. Particularly in external fertilizers, however, sperm limitation through gamete dispersal can also influence gamete investments, but empirical data examining this effect are limited. Here, we studied the relative importance of sperm competition and the spawning conditions in explaining the macroevolutionary patterns of sperm size and number within two taxa with external fertilization but differences in their reproductive biology...
October 4, 2017: Evolution; International Journal of Organic Evolution
Larissa Bomfim, Priscila Vieira, Ariene Fonseca, Isabela Ramos
Most vectors of arthropod-borne diseases produce large eggs with hard and opaque eggshells. In several species, it is still not possible to induce molecular perturbations to the embryo by delivery of molecules using microinjections or eggshell permeabilization without losing embryo viability, which impairs basic studies regarding development and population control. Here we tested the properties and permeability of the eggshell of R. prolixus, a Chagas disease vector, with the aim to deliver pharmacological inhibitors to the egg cytoplasm and allow controlled molecular changes to the embryo...
2017: PloS One
Chang Han Bok, Sung Jun Woo, Chaoxing Wu, Jae Hyeon Park, Tae Whan Kim
Flexible bio-memristive (FBM) devices utilizing chicken egg albumen (CEA):Au@SiO2 core-shell nanoparticle nanocomposites were fabricated on indium-tin-oxide (ITO) coated polyethylene naphthalate (PEN) substrates. Current-voltage (I-V) curves for the Al/CEA:Au@SiO2 core-shell nanoparticle/ITO/PEN devices showed clockwise current hysteresis behaviors due to the existence of the CEA:Au@SiO2 core-shell nanoparticle nanocomposites. The endurance number of the ON/OFF switching for the FBM devices was above 10(2) cycles...
September 20, 2017: Scientific Reports
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