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https://www.readbyqxmd.com/read/28454333/a-polysaccharide-component-from-strongylocentrotus-nudus-eggs-inhibited-hepatocellular-carcinoma-in-mice-by-activating-t-lymphocytes
#1
Min Zhang, Yang Liu, Jingwen Li, Mengyun Ke, Jie Yu, Jie Dou, Hui Wang, Changlin Zhou
A component purified from Strongylocentrotus nudus eggs on a diethylaminoethyl cellulose-52 chromatography column and eluted using a NaCl solution gradient (SEP-S), is a homogeneous polysaccharide of α-D-glucan with a reduced molecular weight of 9.33×10(5) Da, compared with that of S. nudus egg polysaccharide (SEP). In an in vivo antitumor assay of histocompatibility-22 hepatocellular carcinoma in tumor-bearing mice, the inhibitory rates at SEP-S doses of 5, 10 and 20 mg/kg/day were 38.8, 50.7 and 70.3%, respectively...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28453652/mhc-b-variability-within-the-finnish-landrace-chicken-conservation-program
#2
J E Fulton, M E Berres, J Kantanen, M Honkatukia
The major histocompatibility complex (MHC) is a cluster of genes involved with immune responses. The chicken MHC has been shown to influence resistance to viruses, bacteria, and infections from both internal and external parasites. The highly variable chicken MHC haplotypes were initially identified by the use of haplotype-specific serological reagents. A novel SNP-based panel encompassing 210,000 bp of the MHC-B locus was developed to allow fine scale genetic analyses including rapid identification of novel haplotypes for which serological reagents are not available...
April 26, 2017: Poultry Science
https://www.readbyqxmd.com/read/28449065/a-homozygous-missense-mutation-in-eral1-encoding-a-mitochondrial-rrna-chaperone-causes-perrault-syndrome
#3
Iliana A Chatzispyrou, Marielle Alders, Sergio Guerrero-Castillo, Ruben Zapata Perez, Martin A Haagmans, Laurent Mouchiroud, Janet Koster, Rob Ofman, Frank Baas, Hans R Waterham, Johannes N Spelbrink, Johan Auwerx, Marcel M Mannens, Riekelt H Houtkooper, Astrid S Plomp
Perrault syndrome (PS) is a rare recessive disorder characterized by ovarian dysgenesis and sensorineural deafness. It is clinically and genetically heterogeneous, and previously mutations have been described in different genes, mostly related to mitochondrial proteostasis. We diagnosed three unrelated females with PS and set out to identify the underlying genetic cause using exome sequencing. We excluded mutations in the known PS genes, but identified a single homozygous mutation in the ERAL1 gene (c.707A>T; p...
April 25, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28448540/bruchid-egg-induced-transcript-dynamics-in-developing-seeds-of-black-gram-vigna-mungo
#4
Indrani K Baruah, Debashis Panda, Jagadale M V, Deba Jit Das, Sumita Acharjee, Priyabrata Sen, Bidyut Kumar Sarmah
Black gram (Vigna mungo) seeds are a rich source of digestible proteins, however, during storage these seeds are severely damaged by bruchids (Callosobruchus spp.), reducing seed quality and yield losses. Most of the cultivated genotypes of black gram are susceptible to bruchids, however, few tolerant genotypes have also been identified but the mechanism of tolerance is poorly understood. We employed Suppression Subtractive Hybridization (SSH) to identify specifically, but rarely expressed bruchid egg induced genes in black gram...
2017: PloS One
https://www.readbyqxmd.com/read/28447702/structures-in-the-meniscus-of-smectic-membranes-the-role-of-dislocations
#5
M Selmi, J-C Loudet, P V Dolganov, T Othman, P Cluzeau
We report an experimental investigation of the structure of periodic patterns observed in the meniscus of free-standing smectic films. Combination of polarizing optical microscopy and phase shifting interferometry enabled us to obtain new information on the structure of the meniscus, and in particular, on the topography of the smectic-air interface. We investigate the profile of the undulations in the striped structure in the thin part of the meniscus, change of the stripe period with the meniscus thickness and subsequent transition into a two-dimensional structure...
April 27, 2017: Soft Matter
https://www.readbyqxmd.com/read/28447298/a-new-species-of-tinrovia-mamaev-1987-monogenea-microcotylidae-from-the-deep-sea-fish-notacanthus-bonaparte-risso-notacanthiformes-notacanthidae-in-the-western-mediterranean-and-the-north-east-atlantic
#6
Wolf Isbert, Maite Carrassón, Ana Pérez-Del-Olmo, Francisco Esteban Montero
A new microcotylid, Tinrovia mamaevi n. sp. (Monogenea: Polyopisthocotylea), is described from the gills of Notacanthus bonaparte Risso (Notacanthiformes: Notacanthidae), sampled in the Western Mediterranean and North East Atlantic. This species is allocated to the subfamily Syncoelicotylinae Mamaev & Zubchenko, 1978 due to the possession of a symmetrical haptor with two separate frills. The clamps in T. mamaevi n. sp. are of the "microcotylid" type, arranged in two distinct lateral haptoral frills; the genital atrium and the copulatory organ are armed and the vaginal pore is unarmed...
April 26, 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28447071/the-effects-of-reduced-rpd3-levels-on-fly-physiology
#7
Jared K Woods, Tahereh Ziafazeli, Blanka Rogina
BACKGROUND: Rpd3 is a conserved histone deacetylase that removes acetyl groups from lysine residues within histones and other proteins. Reduction or inhibition of Rpd3 extends longevity in yeast, worms, and flies. Previous studies in flies suggest an overlap with the mechanism of lifespan extension by dietary restriction. However, the mechanism of rpd3's effects on longevity remains unclear. OBJECTIVES: In this study we investigated how rpd3 reduction affects fly spontaneous physical activity, fecundity, and stress resistance...
March 31, 2017: Nutrition and Healthy Aging
https://www.readbyqxmd.com/read/28447044/molecular-and-phylogenetic-characterization-of-thelohanellus-boggoti-qadri-1962-cnidaria-myxosporea-bivalvulida-infecting-the-fin-of-indian-minor-carp-labeo-dero-hamilton-1822
#8
Harpreet Kaur, Aditya Gupta
A myxozoan parasite belonging to the genus Thelohanellus Kudo, 1933 (Cnidaria, Myxosporea, Bivalvulida) was isolated from the fins of Labeo dero inhabiting Ranjit Sagar Wetland, Punjab, India. The plasmodium was 0.5-0.7 mm in diameter each containing 80- 100 number of myxospores. Myxospores were egg shaped to ovoidal in valvular view having bluntly pointed anterior and broad rounded posterior end measuring 8.36x 4.77 µm. Polar capsule was single, broadly pyriform in shape, measuring 4.77x3.98 µm in size containing a polar filament coiled perpendicular to the longitudinal axis of myxospore body making 7-9 turns...
March 2017: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28446617/is-embryonic-hypothermia-tolerance-common-in-birds
#9
Jin-Ming Zhao, Zhi-Ming Han, Yue-Hua Sun
Avian incubation temperatures oscillate within narrow limits to ensure proper embryonic development. However, field observations and experimental studies have found that some species can tolerate very low incubation temperatures, either regularly or occasionally. We artificially incubated eggs from five domestic species, which represent a range of egg sizes, to examine whether a diversity of avian species could exhibit an unusual hypothermia tolerance, as observed in the field. We found that eggs of the chicken (Gallus gallus domesticus), pigeon (Columba livia domestica), Japanese quail (Coturnix japonica) and budgerigar (Melopsittacus undulatus) survived the incubation period and hatched after experiencing 10°C hypothermia for 6 h each day...
April 2017: Biology Letters
https://www.readbyqxmd.com/read/28446604/cell-cycle-dependent-regulation-of-greatwall-kinase-by-protein-phosphatase-1-and-regulatory-subunit-3b
#10
Dapeng Ren, Laura A Fisher, Jing Zhao, Ling Wang, Byron C Williams, Michael L Goldberg, Aimin Peng
Greatwall (Gwl) kinase plays an essential role in regulation of mitotic entry and progression. Mitotic activation of Gwl requires both cyclin-dependent kinase 1 (CDK1)-dependent phosphorylation and its autophosphorylation at an evolutionarily-conserved serine residue near the carboxyl-terminus (Ser-883 in Xenopus). In this study we show that Gwl associates with protein phosphatase 1 (PP1), particularly PP1γ, which mediates the dephosphorylation of Gwl Ser-883. Consistent with the mitotic activation of Gwl, its association with PP1 is disrupted in mitotic cells and egg extracts...
April 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28446499/food-groups-and-risk-of-all-cause-mortality-a-systematic-review-and-meta-analysis-of-prospective-studies
#11
Lukas Schwingshackl, Carolina Schwedhelm, Georg Hoffmann, Anna-Maria Lampousi, Sven Knüppel, Khalid Iqbal, Angela Bechthold, Sabrina Schlesinger, Heiner Boeing
Background: Suboptimal diet is one of the most important factors in preventing early death and disability worldwide.Objective: The aim of this meta-analysis was to synthesize the knowledge about the relation between intake of 12 major food groups, including whole grains, refined grains, vegetables, fruits, nuts, legumes, eggs, dairy, fish, red meat, processed meat, and sugar-sweetened beverages, with risk of all-cause mortality.Design: We conducted a systematic search in PubMed, Embase, and Google Scholar for prospective studies investigating the association between these 12 food groups and risk of all-cause mortality...
April 26, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28445889/genetic-polymorphisms-in-aromatase-cyp19a1-are-not-associated-with-gonadal-phenotypes-in-red-eared-slider-turtle-hatchlings-developed-at-a-pivotal-temperature
#12
Yuiko Matsumoto, David Crews
Embryonic gonad sex in many reptilian species is determined by the incubation temperature of the egg, a differentiation process known as temperature-dependent sex determination (TSD). Incubation at the pivotal temperature (PvT) results in approximately an equal number of offspring of both sexes. We investigated the potential contribution of genetic variations that drives the gonadal differentiation into testes or ovaries under this temperature in the red-eared slider turtle (Trachemys scripta). Four male and 4 female hatchlings of eggs that had been incubated at the PvT were examined for polymorphisms at an approximately 23-kb region of the aromatase (cyp19a1) gene...
April 27, 2017: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
https://www.readbyqxmd.com/read/28445717/hepatic-elovl6-mrna-is-regulated-by-the-gga-mir-22-3p-in-egg-laying-hen
#13
Zheng Ma, Hong Li, Hang Zheng, Keren Jiang, Fengbin Yan, Yadong Tian, Xiangtao Kang, Yanbin Wang, Xiaojun Liu
The elongation of very long chain fatty acids protein 6 (ELOVL6) encodes a fatty acid elongase that is responsible for the final step in endogenous saturated fatty acid synthesis and involves in de novo lipogenesis. Though the regulatory mechanism of ELOVL6 expression has been studied extensively, little is known about the role of miRNA in regulating ELOVL6 gene expression in chicken until now. To investigate the regulatory mechanism of miRNA on the expression of ELOVL6 gene, bioinformatics analysis was employed to predict the potential miRNAs that binding with the 3'untranslated region (3'UTR) of ELOVL6...
April 23, 2017: Gene
https://www.readbyqxmd.com/read/28444827/fitness-costs-and-life-table-parameters-of-highly-insecticide-resistant-strains-of-plutella-xylostella-l-lepidoptera-plutellidae-at-different-temperatures
#14
Denise Steinbach, Gerald Moritz, Ralf Nauen
BACKGROUND: In many cases resistance alleles have been associated with fitness costs and are often dependent on environmental factors such as temperature. Here, we studied the effects of temperature on the overall fitness, including development, survival, and reproduction of three insecticide resistant and one susceptible strain of diamondback moth (DBM), Plutella xylostella (L.). RESULTS: The broader cross-resistance profile of the resistant strains previously selected by diamide and benzoylurea insecticides was tested...
April 26, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28444789/effect-of-dietary-supplementation-of-hemp-seed-cannabis-sativa-l-on-meat-quality-and-egg-fatty-acid-composition-of-japanese-quail-coturnix-coturnix-japonica
#15
H Yalcin, Y Konca, F Durmuscelebi
This study was conducted in two separate experiments. Experiment I was conducted to investigate the effects of hemp seed (HS) on meat quality traits, and experiment II was designed to determine egg fatty acid (FA) composition in Japanese quail (Coturnix coturnix japonica). In experiment I and experiment II treatments, groups were as follows: (i) control (not included HS0), (ii) 5% HS inclusion to diets, (iii) 10% HS inclusion to diets and (iv) 20% HS inclusion to diets. In experiment I, a total of 192 7-day-old-quail were fed with HS diets for 5 weeks, and end of the experiment I, a total of 64 quail (16 each) slaughtered and meat samples were analysed for meat colour, pH, cooking and thawing loss and FA composition...
April 26, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28444421/metal-levels-in-shorebird-feathers-and-blood-during-migration-through-delaware-bay
#16
Nellie Tsipoura, Joanna Burger, Lawrence Niles, Amanda Dey, Michael Gochfeld, Mark Peck, David Mizrahi
We investigated levels of arsenic mercury, lead, cadmium, and chromium in Red Knot (Calidris canutus), Semipalmated Sandpipers (Calidris pusilla), and Sanderling (Calidris alba) migrating through Delaware Bay, New Jersey to determine if contaminant levels are likely to be causing negative effects on the populations of these shorebirds, to compare among species, and to explore differences among individuals collected early and late during their migration stopover. We analyzed blood and feathers, both nonlethal ways of exploring contaminants in birds...
April 25, 2017: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28444375/evaluation-of-the-alpha-amylase-activity-as-an-indicator-of-pasteurization-efficiency-and-microbiological-quality-of-liquid-whole-eggs
#17
Guilherme Resende da Silva, Liliane Denize Miranda Menezes, Isabela Pereira Lanza, Daniela Duarte de Oliveira, Carla Aparecida Silva, Roger Wilker Tavares Klein, Débora Cristina Sampaio de Assis, Silvana de Vasconcelos Cançado
In order to evaluate the efficiency of the pasteurization process in liquid whole eggs, an UV/visible spectrophotometric method was developed and validated for the assessment of alpha-amylase activity. Samples were collected from 30 lots of raw eggs (n = 30) and divided into three groups: one was reserved for analysis of the raw eggs, the second group was pasteurized at 61.1°C for 3.5 minutes (n = 30), and the third group was pasteurized at 64.4°C for 2.5 minutes (n = 30). In addition to assessing alpha-amylase activity, the microbiological quality of the samples was also evaluated by counting total and thermotolerant coliforms, mesophilic aerobic microorganisms, Staphylococcus spp...
April 24, 2017: Poultry Science
https://www.readbyqxmd.com/read/28444234/inferring-allele-frequency-trajectories-from-ancient-dna-indicates-that-selection-on-a-chicken-gene-coincided-with-changes-in-medieval-husbandry-practices
#18
Liisa Loog, Mark G Thomas, Ross Barnett, Richard Allen, Naomi Sykes, Ptolemaios D Paxinos, Ophelie Lebrasseur, Keith Dobney, Joris Peters, Andrea Manica, Greger Larson, Anders Eriksson
Ancient DNA provides an opportunity to infer the drivers of natural selection by linking allele frequency changes to temporal shifts in environment or cultural practices. However, analyses have often been hampered by uneven sampling and uncertainties in sample dating, as well as being confounded by demographic processes. Here we present a Bayesian statistical framework for quantifying the timing and strength of selection using ancient DNA that explicitly addresses these challenges. We applied this method to time series data for two loci: TSHR and BCDO2, both argued to have undergone strong and recent selection in domestic chickens...
April 21, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28443983/taenia-sp-in-human-burial-from-kan-river-east-siberia
#19
Sergey Mikhailovich Slepchenko, Sergey Nikolaevich Ivanov, Anton Vasilevich Vybornov, Tsybankov Alexander Alekseevich, Slavinsky Vyacheslav Sergeyevich, Danil Nikolaevich Lysenko, Vyacheslav Evgenievich Matveev
We present an arhaeoparasitological analysis of a unique burial from the Neftprovod II burial ground in East Siberia, which dated from the Bronze Age. Analysis of a sediment sample from the sacral region of the pelvis revealed the presence of Taenia sp. eggs. Because uncooked animal tissue is the primary source of Taenia, this indicated that the individual was likely consuming raw or undercooked meat of roe deer, red deer, or elk infected with Taenia. This finding represents the oldest case of a human infected with Taenia sp...
May 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28443982/enzyme-linked-immunosorbent-assay-for-diagnosis-of-amphimerus-spp-liver-fluke-infection-in-humans
#20
William Cevallos, Manuel Calvopiña, Victoria Nipáz, Belén Vicente-Santiago, Julio López-Albán, Pedro Fernández-Soto, Ángel Guevara, Antonio Muro
BACKGROUND: Amphimerus spp. is a liver fluke that infects humans and domestic animals. It is highly prevalent in some Ecuadorian communities. Currently, diagnosis is based on the microscopic observation of eggs in faeces, but this has variable sensitivity. More sensitive methods are needed for diagnostic testing. OBJECTIVE: The main objective of this work was to develop an enzyme-linked immunosorbent assay (ELISA) using crude antigens from Amphimerus spp. adult worms to detect anti-Amphimerus IgG in human sera...
May 2017: Memórias do Instituto Oswaldo Cruz
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