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https://www.readbyqxmd.com/read/29331592/combined-creatinine-cystatin-c-ckd-epi-equation-significantly-underestimates-measured-glomerular-filtration-rate-in-people-with-type-2-diabetes-mellitus
#1
Júlia Dauernheimer Machado, Eduardo Guimarães Camargo, Roberta Boff, Laís da Silva Rodrigues, Joíza Lins Camargo, Ariana Aguiar Soares, Sandra Pinho Silveiro
AIM: To evaluate the accuracy of creatinine and cystatin C (cysC) equations to estimate glomerular filtration rate (GFR) in type 2 diabetes mellitus (DM) patients and healthy adults. METHODS: Case-control study including 84 patients with type 2 DM and 100 healthy adults with measured GFR (mGFR)≥60mL/min/1.73m2. GFR was measured by 51Cr-EDTA and estimated (eGFR) by the following equations using creatinine, cysC or both markers: Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI), Caucasian Asian Pediatrics and Adults (CAPA), CKD-EPI creatinine-cystatin C (CKDEPI-CC), and CKD-EPI cystatin C (CKDEPIcysC)...
January 10, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29329981/molecular-cloning-and-characterization-of-leucine-aminopeptidase-gene-from-taenia-pisiformis
#2
Shaohua Zhang, Xuepeng Cai, Xuenong Luo, Shuai Wang, Aijiang Guo, Junling Hou, Run Wu
Leucine aminopeptidase (LAP, EC: 3.4.11.1) is an important metalloexopeptidase that catalyze the hydrolysis of amino-terminal leucine residues from polypeptides and proteins. In this study, a full length of cDNA encoding leucine aminopeptidase of Taenia pisiformis (TpLAP) was cloned by rapid amplification of cDNA-ends using the polymerase chain reaction (RACE-PCR) method. The full-length cDNA of the TpLAP gene is 1823 bp and contains a 1569 bp ORF encoding 533 amino acids with a putative mass of 56.4 kDa...
January 9, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29329710/hydride-generation-coupled-with-thioglycolic-acid-coated-gold-nanoparticles-as-simple-and-sensitive-headspace-colorimetric-assay-for-visual-detection-of-sb-iii
#3
Tesfaye Tolessa, Zhi-Qiang Tan, Jing-Fu Liu
Antimony (Sb) is a toxic element which causes different health problems including cardiac problems and lung cancer in humans, and its levels in surface water can be noticeably increased to 100 μg/L typically in the proximity of anthropogenic sources. Thus, besides instrumental techniques, it is of great significance to develop a simple, sensitive and selective analytical method for direct analysis of Sb(III) at trace level without the need of any expensive and/or complicated instrumentations and sample preparation processes...
April 3, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29329231/development-of-a-cytocompatible-scaffold-from-pig-immature-testicular-tissue-allowing-human-sertoli-cell-attachment-proliferation-and-functionality
#4
Maxime Vermeulen, Federico Del Vento, Francesca de Michele, Jonathan Poels, Christine Wyns
Cryopreservation of immature testicular tissue before chemo/radiotherapy is the only option to preserve fertility of cancer-affected prepubertal boys. To avoid reintroduction of malignant cells, development of a transplantable scaffold by decellularization of pig immature testicular tissue (ITT) able to support decontaminated testicular cells could be an option for fertility restoration in these patients. We, therefore, compared decellularization protocols to produce a cytocompatible scaffold. Fragments of ITT from 15 piglets were decellularized using three protocols: sodium dodecyl sulfate (SDS)-Triton (ST), Triton-SDS-Triton (TST) and trypsin 0...
January 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29329098/effect-of-organic-amendments-on-cadmium-stress-to-pea-a-multivariate-comparison-of-germinating-vs-young-seedlings-and-younger-vs-older-leaves
#5
Saliha Shamshad, Muhammad Shahid, Marina Rafiq, Sana Khalid, Camille Dumat, Muhammad Sabir, Behzad Murtaza, Abu Bakr Umer Farooq, Noor S Shah
Despite significant recent advancement in research, biogeochemical behavior of heavy metals with respect to their applied form is still topical. Moreover, metal toxicity to plants may vary with their stage of development/maturity. Therefore, this study for the first time evaluated the influence of ethylenediaminetetraacetic acid (EDTA) and citric acid (CA) on cadmium (Cd) accumulation and toxicity to germinating and young pea seedlings as well as in younger and older leaves. The experimental setup of current study consisted of two separate studies...
January 9, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29329026/the-interaction-of-pyridoxal-isonicotinoyl-hydrazone-pih-and-salicylaldehyde-isonicotinoyl-hydrazone-sih-with-iron
#6
Yu-Lin Chen, Xiaole Kong, Yuanyuan Xie, Robert C Hider
The interaction of pyridoxal isonicotinoyl hydrazone (PIH) and salicylaldehyde isonicotinoyl hydrazone (SIH), two important biologically active chelators, with iron has been investigated by spectrophotometric methods. High iron(III) affinity constants were determined for PIH, logβ2=37.0 and SIH, logβ2=37.6. The associated redox potentials of the iron complexes were determined using cyclic voltammetry at pH7.4 as +130mV (vs normal hydrogen electrode, NHE) for PIH and +136mV(vs NHE) for SIH. These redox potentials are much higher than those corresponding to iron chelators in clinical use, namely deferiprone, -620mV; desferasirox, -600mV and desferrioxamine, -468mV...
December 21, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29328981/a-simple-lc-ms-ms-method-for-determination-of-deferasirox-in-human-plasma-troubleshooting-of-interference-from-ferric-ion-in-method-development-and-its-application
#7
Tengfei Li, Zhimin Cui, Yan Wang, Wen Yang, Duo Li, QinXin Song, Luning Sun, Li Ding
As an orally active iron chelator, deferasirox forms its ion complexes in the prepared plasma samples and LC-MS mobile phase where ferric ion exists, and then comparing with the nominal concentration level, a lower detected concentration level of deferasirox would be obtained after LC-MS analysis, if no proper treatment was adopted. Meanwhile, the phenomenon would be observed that multiple repeat injections of the same deferasirox plasma sample in the same tube would show the lower and lower detected concentration levels of deferasirox, which caused by more and more ferric ions from the injection needle dissolved in the sample solution as multiple repeated injections...
December 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29326515/scanning-electron-microscopic-evaluation-of-efficacy-of-17-ethylenediaminetetraacetic-acid-and-chitosan-for-smear-layer-removal-with-ultrasonics-an-in-vitro-study
#8
Aradhana Babu Kamble, Sathish Abraham, Deepak Dadarao Kakde, C Shashidhar, Disha Lalit Mehta
Introduction: The main aim of root canal treatment is cleaning, shaping and then obturating three dimensionally to prevent reinfection. This includes chemicomechanical cleansing by instrumentation and the use of irrigating solutions. Therefore, the purpose of this study was to compare the smear layer removal from root canal dentine subjected to two root canal irrigants, 17% EDTA and 0.2% Chitosan, a new irrigant using Scanning Electron Microscope. Methodology: 40 single rooted premolars were decoronated followed by instrumentation with I Race files and intermediate irrigation with 3% sodium hypochlorite and activation with ultrasonics...
October 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/29325746/first-report-of-the-characterization-of-a-snake-venom-apyrase-ruviapyrase-from-indian-russell-s-viper-daboia-russelii-venom
#9
Bhargab Kalita, Aparup Patra, Shagufta Jahan, Ashis K Mukherjee
A novel apyrase from Russell's viper venom (RVV) was purified and characterized, and it was named Ruviapyrase (Russell's viper apyrase). It is a high molecular weight (79.4 kDa) monomeric glycoprotein that contains 2.4% neutral sugars and 58.4% N-linked oligosaccharides and strongly binds to Concanavalin A. The LC-MS/MS analysis did not identify any protein in NCBI protein database, nevertheless some de novo sequences of Ruviapyrase showed putative conserved domain of apyrase superfamily. Ruviapyrase hydrolysed adenosine triphosphate (ATP) to a significantly greater extent (p < ...
January 8, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29319814/single-versus-multiple-sample-method-to-measure-glomerular-filtration-rate
#10
Pierre Delanaye, Martin Flamant, Laurence Dubourg, Emmanuelle Vidal-Petiot, Sandrine Lemoine, Etienne Cavalier, Elke Schaeffner, Natalie Ebert, Hans Pottel
Background: There are many different ways to measure glomerular filtration rate (GFR) using various exogenous filtration markers, each having their own strengths and limitations. However, not only the marker, but also the methodology may vary in many ways, including the use of urinary or plasma clearance, and, in the case of plasma clearance, the number of time points used to calculate the area under the concentration-time curve, ranging from only one (Jacobsson method) to eight (or more) blood samples...
January 8, 2018: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29319507/inhibition-of-multidrug-resistant-gram-positive-and-gram-negative-bacteria-by-a-photoactivated-porphyrin
#11
Moreno Bondi, Anna Mazzini, Simona de Niederhäusern, Ramona Iseppi, Patrizia Messi
The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable susceptibility of S. aureus and E. faecalis to treatment while, under the same conditions, E. coli and P. aeruginosa showed very low susceptibility...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29319417/control-of-h2s-generation-in-simultaneous-removal-of-no-and-so2-by-rotating-drum-biofilter-coupled-with-feii-edta
#12
Jun Chen, Bingbin Li, Ji Zheng, Jianmeng Chen
Simultaneous removal of SO2 and NO from flue gas can be biologically achieved by the rotating drum biofilter with FeII(EDTA) as a solvent. One issue related with this process is the generation of H2S. To control its generation, the pathways of H2S formation was investigated, and the parametric tests were conducted. The addition of FeII(EDTA) (10 mM) increased the removal efficiency of NO and SO2, while the S2- concentration decreased from 81 mM to 3.5 mM. The parametric tests showed that the high NO concentration favored the SO2 removal and significantly inhibited the H2S generation...
January 10, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29318027/diagnostic-performances-of-two-rapid-tests-for-detection-of-feline-leukemia-virus-antigen-in-sera-of-experimentally-feline-leukemia-virus-infected-cats
#13
Matthew R Krecic, Sridhar Velineni, Patrick Meeus, Huihao Fan, Michael Loenser
Objectives: The objective of this study was to compare the diagnostic sensitivities and specificities of WITNESS FeLV-FIV (Zoetis) and SNAP FIV/FeLV Combo Test (IDEXX) for the detection of FeLV p27 antigen in the sera of experimentally feline leukemia virus (FeLV)-infected cats. Methods: Diagnostic sensitivities of WITNESS and SNAP were determined through testing of 47 serum samples collected from cats day 56 post-experimental infection with a virulent FeLV Rickard strain...
January 2018: JFMS Open Reports
https://www.readbyqxmd.com/read/29315768/the-effect-of-uric-acid-lowering-treatment-on-albuminuria-and-renal-function-in-type-1-diabetes-a-randomized-clinical-trial
#14
S Pilemann-Lyberg, F Persson, J Frystyk, P Rossing
Significant improvements have been made in the management of Type 1 diabetes over the last three decades [1]. We wanted to examine if uric acid lowering with allopurinol changes the urinary albumin excretion rate (UAER) or 51 Cr EDTA GFR in people with Type 1 diabetes and diabetic nephropathy. This article is protected by copyright. All rights reserved.
January 7, 2018: Diabetic Medicine: a Journal of the British Diabetic Association
https://www.readbyqxmd.com/read/29315029/correlations-among-resistances-to-different-antimicrobial-compounds-in-salmonella-strains-from-hen-eggshells
#15
Maria Luisa Fernández Márquez, María José Grande Burgos, Rubén Pérez Pulido, Antonio Gálvez, Rosario Lucas López
Persistence of antibiotic-resistant Salmonella in the food chain may depend on strain tolerance to other antimicrobials and also on biofilm formation capacity. Yet, there is limited information on sensitivity of antibiotic-resistant Salmonella to other antimicrobials, such as phenolic compounds, chemical preservatives, or antimicrobial peptides. This study aimed at correlating antimicrobial resistance and biofilm formation capacity in antibiotic-resistant, biocide-tolerant Salmonella strains from hen eggshells...
February 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29313389/multi-target-stool-dna-test-in-the-surveillance-of-inflammatory-bowel-disease-a-cross-sectional-cohort-study
#16
Pasquale Klepp, John B Kisiel, Milada Cvancarova Småstuen, Arne Røseth, Solveig N Andersen, Morten H Vatn, David A Ahlquist, Bjørn A Moum, Stephan Brackmann
BACKGROUND AND AIM: Colonoscopic surveillance is recommended in patients with longstanding inflammatory bowel disease (IBD) as they are at increased risk of colorectal cancer (CRC). Non-invasive surveillance may improve compliance and access. Multi-target stool DNA (MT-sDNA) has been validated for screening of sporadic CRC but has not been assessed in IBD. Our aim was to assess the performance of a MT-sDNA test in a real-life surveillance setting of patients with longstanding IBD. MATERIAL AND METHODS: A total of 192 IBD patients enrolled from two prospective cohorts submitted an EDTA buffered stool sample and underwent chromo- or white light colonoscopy...
January 9, 2018: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/29313383/comparison-of-decalcifying-agents-and-techniques-for-human-dental-tissues
#17
A Choube, M Astekar, A Choube, G Sapra, A Agarwal, A Rana
Teeth are among the hardest animal tissues, because they are composed of large amounts of inorganic compounds. Consequently, teeth are difficult to prepare for microscopic examination. Acids and chelating agents traditionally have been used to remove calcium ions. We compared decalcifying agents including strong acids, weak acids, chelating agents, techniques using electric current, agitation and heat. Freshly extracted teeth were fixed and decalcified using formic acid-formalin, formal-nitric acid, formalin-EDTA, Von Ebner's fluid and Perenyi's fluid...
January 9, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29310313/quality-monitoring-methods-of-initial-and-terminal-manufacture-of-lifepo4-based-lithium-ion-batteries-by-capillary-electrophoresis
#18
Xia Xie, Yang Yang, Henghui Zhou, Meixian Li, Zhiwei Zhu
Magnetic impurities of lithium ion battery degrade both the capacity and cycling rates, even jeopardize the safety of the battery. During the material manufacture of LiFePO4, two opposite and extreme cases (trace impurity Fe(II) with high content of Fe(III) background in FePO4 of initial end and trace Fe(III) with high content of Fe(II) background in LiFePO4 of terminal end) can result in the generation of magnetic impurities. Accurate determination of impurities and precise evaluation of raw material or product are necessary to ensure reliability, efficiency and economy in lithium ion battery manufacture...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29306536/orthodontic-movement-after-regenerative-endodontic-procedure-case-report-and-long-term-observations
#19
Antonis Chaniotis
INTRODUCTION: Although regenerative treatment approaches in teeth with incomplete root formation and pulp necrosis have become part of the suggested therapeutic endodontic spectrum, little is known about the effect of orthodontic movement in the tissue that has been regenerated. Furthermore, as the number of adults undergoing orthodontic treatment increases, there is an increasing need to investigate the changes that these tissues may undergo during orthodontic movement. Here we describe the alterations observed after the application of orthodontic forces in a case of an apically root-fractured necrotic immature root that had been managed with regenerative endodontic procedures in the past...
January 3, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29305959/enhanced-in-vitro-tigecycline-activity-in-the-presence-of-chelating-agents
#20
Amelia N Deitchman, Ravi Shankar Prasad Singh, Kenneth H Rand, Hartmut Derendorf
Lack of availability of novel antibiotic agents and the rise of resistance to existing therapies has led clinicians to utilize combination therapy to adequately treat bacterial infections. Here we examine how chelators may impact the in vitro activity of tigecycline (TIG) against Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae. Minimum inhibitory concentrations (MICs) were determined by broth dilution with and without various combinations of chelators (EDTA and other tetracyclines), and metal ions (i...
January 3, 2018: International Journal of Antimicrobial Agents
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