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https://www.readbyqxmd.com/read/28816641/preparation-of-recombinant-human-thymic-stromal-lymphopoietin-protein
#1
Yanlu Zhang, Liehua Wu, Jingai Yu, JianFeng Mei, Yu Yi, JianShu Chen, Guoqing Ying
Human thymic stromal lymphopoietin (hTSLP) protein plays a central role in inflammation. Characterizing properties of hTSLP requires a recombinant overexpression system that produces correctly folded, active hTSLP. In this report, an efficient overexpression system for production of hTSLP was developed. We constructed expression plasmids of the full-length hTslp gene with or without the signal peptide and transformed the plasmids into E. coli. The design of the recombinant proteins included an N-terminal His-tag, which facilitated purification...
August 17, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28816110/reduced-beta-amyloid-sensitivity-for-platelet-monocyte-aggregates-in-edta-blood-of-alzheimer-patients
#2
Michaela Defrancesco, Josef Marksteiner, Christian Humpel
Alzheimer´s disease (AD) is a severe neurodegenerative brain disorder characterized by beta-amyloid plaques, Tau pathology, inflammation, neurodegeneration, and cerebrovascular dysfunction. Besides that, alterations in monocytes and platelets have been reported in the blood of Alzheimer patients. In the present study, we measured circulating levels of platelet-monocyte aggregates in EDTA blood of cognitively healthy participants and 40 AD patients, and examined their changes induced by stimulation with beta-amyloid peptides...
August 17, 2017: International Psychogeriatrics
https://www.readbyqxmd.com/read/28815247/-changes-of-cementum-endotoxin-levels-in-different-teeth-with-periodontitis-treated-with-root-conditioning
#3
Chang-Sheng Fu, Rong-Sen Liu, Yun Luo, Long Ou, Ying-Chao Li, Xian-Hua Zhang
PURPOSE: To observe the changes of endotoxin levels after different teeth with periodontitis were treated with different methods. METHODS: Six healthy premolars extracted for orthodontic reasons and 36 posterior teeth extracted due to severe periodontitis were selected. Each tooth was processed from two 4 mm×4 mm×1 mm cementum pieces 2 mm under the cementum-enamel junction, each tooth with periodontitis was numbered. Healthy teeth served as negative control group, one of the two tablets from each tooth with periodontitis was selected in the periodontitis group, which was not treated with root surface treatment...
April 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/28815245/-experimental-study-on-osteogenesis-of-acellular-porcine-pericardium-in-guided-bone-regeneration
#4
Jun-Zhao Gao, Xue-Jian Zhang, Jia-Li Hou, Yue Deng, Yun-Peng Li
PURPOSE: To observe the retention time and effect of acellular porcine pericardium (APP) in bone regeneration for rabbit femoral bone defects. METHODS: APP was prepared as follows: fresh porcine pericardium was chosen, high and low osmotic NaCl solution were used to soak the specimen alternately, trypsin+EDTA were used for digest, and then immersed with TritonX-100, cross-linked by using glutaraldehyde. 24 New Zealand rabbits were selected, and the area of bone defect was created 8 mm×8 mm×5 mm in size on bilateral distal femur...
April 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/28814396/consumption-of-galacto-oligosaccharides-increases-iron-absorption-from-a-micronutrient-powder-containing-ferrous-fumarate-and-sodium-iron-edta-a-stable-isotope-study-in-kenyan-infants
#5
Daniela Paganini, Mary A Uyoga, Colin I Cercamondi, Diego Moretti, Edith Mwasi, Clarissa Schwab, Salome Bechtler, Francis M Mutuku, Valeria Galetti, Christophe Lacroix, Simon Karanja, Michael B Zimmermann
Background: Whether consumption of prebiotics increases iron absorption in infants is unclear.Objective: We set out to determine whether prebiotic consumption affects iron absorption from a micronutrient powder (MNP) containing a mixture of ferrous fumarate and sodium iron EDTA (FeFum+NaFeEDTA) in Kenyan infants.Design: Infants (n = 50; aged 6-14 mo) consumed maize porridge that was fortified with an MNP containing FeFum+NaFeEDTA and 7.5 g galacto-oligosaccharides (GOSs) (Fe+GOS group, n = 22) or the same MNP without GOSs (Fe group, n = 28) each day for 3 wk...
August 16, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28811773/transepithelial-corneal-crosslinking-in-treatment-of-progressive-keratoconus-12-months-clinical-results
#6
Bushra Akbar, Rana Intisar-Ul-Haq, Mazhar Ishaq, Sabahat Arzoo, Kashif Siddique
OBJECTIVE: The purpose of this study was to evaluate the safety and efficacy of transepithelial corneal collagen cross linking (TE-CXL) with modified riboflavin and accelerated UVA irradiance in thin corneas with pachymetry less than 400 microns at thinnest point, untreatable by epithelium off corneal collagen cross linking (CXL) in adult Pakistani population with progressive keratoconus. METHODS: This quasi experimental study included twenty six eyes of 26 patients with progressive keratoconus who underwent accelerated transepithelial CXL in Armed forced institute of ophthalmology with 12 months follow up...
May 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28810293/-comparative-study-of-three-different-decalcifying-solutions-in-bone-metastasis-specimens-with-breast-cancer
#7
H Y Wu, T Wang, K L Chen, Z W Fan, X H Pu, B Zhang, X S Fan, F Q Meng
Objective: To investigate the optimal strategy for immunohistochemical (IHC) staining in bone metastasis specimens from breast cancer. Methods: Twenty-eight bone metastases specimens from breast cancers were divided into three groups and subjected to different decalcifying agents (group A-10% nitrate, group B-EDTA decalcification, and group C-imported decalcifying solution RapidCal). The effects of those on HE and IHC staining for Ki-67, ER, PR, GATA3, RANK, RANKL, HER2 and HER2 FISH results were assessed. Results: There were no significant differences among three groups in HE morphology and IHC staining...
August 8, 2017: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/28808673/data-on-the-impact-of-the-blood-sample-collection-methods-on-blood-protein-profiling-studies
#8
Maria Ilies, Cristina Adela Iuga, Felicia Loghin, Vishnu Mukund Dhople, Thomas Thiele, Uwe Völker, Elke Hammer
Complete blood protein profiles of 4 different blood sample collection methods (EDTA-, heparin- and citrate plasma and serum) were investigated and the data presented herein is an extension of the research article in Ilies et al. [1]. Specimens were depleted of 6 highly abundant proteins and protein profiling was assessed by nano-LC UDMS(E). Exhaustive protein sets and protein abundances before and after depletion are presented in tables and figures. Also, the core protein set and the unique proteins for each sample collection method previously described [1] are disclosed...
October 2017: Data in Brief
https://www.readbyqxmd.com/read/28806652/applicability-of-alkaline-precipitation-for-the-recovery-of-edds-spent-solution
#9
Marco Race
This paper presents an innovative procedure for the recovery of SS-ethylenediamine-N,N'-disuccinic acid (EDDS) solution used for soil washing processes. The procedure is derived from that applied for the recovery of ethylenediamine-tetraacetic acid (EDTA), modifying and optimizing the choice of the chemical agents used for the protonation of the chelant, the exchange of the metals, and the final precipitation of the unwanted compounds. To select the reagents and test the proposed approach, an experimental study was conducted on real EDDS spent solutions, obtained washing a Cu and Zn real contaminated soil...
August 11, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28803155/assisted-phytoremediation-of-a-multi-contaminated-soil-investigation-on-arsenic-and-lead-combined-mobilization-and-removal
#10
Meri Barbafieri, Francesca Pedron, Gianniantonio Petruzzelli, Irene Rosellini, Elisabetta Franchi, Roberto Bagatin, Marco Vocciante
The removal of contaminants from an earthy matrix by phytoremediation requires the selection of appropriate plant species and a suitable strategy to be effective. In order to set up an assisted phytoremediation intervention related to a disused industrial site affected by an arsenic and lead complex contamination, an extensive experimental investigation on micro and mesocosm scale has been conducted. Particular attention was given to the choice of plant species: using crop plants (Lupinus albus, Helianthus annuus and Brassica juncea) a series of parallel test campaigns have been realized to investigate different scenarios for the reclamation...
August 10, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28802027/performance-evaluation-of-the-bioneer-accupower%C3%A2-hiv-1-quantitative-rt-pcr-kit-comparison-with-the-roche-cobas%C3%A2-ampliprep-cobas-taqman%C3%A2-hiv-1-test-ver-2-0-for-quantification-of-hiv-1-viral-load-in-indonesia
#11
Agus Susanto Kosasih, Christine Sugiarto, Hubertus Hosti Hayuanta, Runingsih Juhaendi, Lyana Setiawan
BACKGROUND: Measurement of viral load in human immunodeficiency virus type 1 (HIV-1) infected patients is essential for the establishment of a therapeutic strategy. Several assays based on qPCR are available for the measurement of viral load; they differ in sample volume, technology applied, target gene, sensitivity and dynamic range. The Bioneer AccuPower® HIV-1 Quantitative RT-PCR is a novel commercial kit that has not been evaluated for its performance. OBJECTIVE: This study aimed to evaluate the performance of the Bioneer AccuPower® HIV-1 Quantitative RT-PCR kit...
August 8, 2017: Asian Pacific Journal of Allergy and Immunology
https://www.readbyqxmd.com/read/28801864/isolation-and-characterization-of-iron-chelators-from-turmeric-curcuma-longa-selective-metal-binding-by-curcuminoids
#12
Donald J Messner, Christine Surrago, Celia Fiordalisi, Wing Yin Chung, Kris V Kowdley
Iron overload disorders may be treated by chelation therapy. This study describes a novel method for isolating iron chelators from complex mixtures including plant extracts. We demonstrate the one-step isolation of curcuminoids from turmeric, the medicinal food spice derived from Curcuma longa. The method uses iron-nitrilotriacetic acid (NTA)-agarose, to which curcumin binds rapidly, specifically, and reversibly. Curcumin, demethoxycurcumin, and bisdemethoxycurcumin each bound iron-NTA-agarose with comparable affinities and a stoichiometry near 1...
August 11, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28800536/fate-of-metals-before-and-after-chemical-extraction-of-incinerated-sewage-sludge-ash
#13
Jiang-Shan Li, Daniel C W Tsang, Qi-Ming Wang, Le Fang, Qiang Xue, Chi Sun Poon
Chemical extraction of incinerated sewage sludge ash (ISSA) can effectively recycle P, but it may change the speciation and mobility of the remaining metals. This study investigated the changes of the leaching potential and distribution of metals in the chemically extracted ISSA. Batch extraction experiments with different extractants, including inorganic acids, organic acids, and chelating agents, were conducted on the ISSA collected from a local sewage sludge incinerator. The extraction of Zn, Cu, Pb, Ni, Cd, Ba, Cr and As from the ISSA and the corresponding changes of the mobility and speciation were examined...
August 5, 2017: Chemosphere
https://www.readbyqxmd.com/read/28795145/quantitative-evaluation-of-the-impact-of-ethylenediaminetetraacetic-acid-pretreatment-on-single-antigen-bead-assay
#14
Chang Liu, Sue Pang, Donna Phelan, Daniel C Brennan, Thalachallour Mohanakumar
BACKGROUND: Ethylenediaminetetraacetic acid (EDTA) pretreatment has been shown to overcome complement interference in the single-antigen bead (SAB) assay. However, a quantitative evaluation of its impact on the assay for preemptive application to diverse clinical samples is still lacking. METHODS: Serum samples from 95 renal transplant candidates were tested with and without EDTA-pretreatment in parallel. Changes in mean fluorescence intensity (MFI) values were analyzed to determine the impact of EDTA-pretreatment and the characteristics of complement interference...
August 2017: Transplantation Direct
https://www.readbyqxmd.com/read/28783138/flow-cytometric-sorting-coupled-with-exon-capture-sequencing-identifies-somatic-mutations-in-archival-lymphoma-tissues
#15
Nenggang Jiang, Christopher Chen, Qiang Gong, Kristen Shields, Yuping Li, YuanYuan Chen, Joo Song, Timothy W McKeithan, Wing C Chan
The enormous number of archived formalin-fixed paraffin-embedded (FFPE) tissues available are a valuable resource of material for research. However, the use of such tissues poses many challenges, among which is the difficulty of isolating different cell populations within the tissue. In this study, we used tissue from two types of non-Hodgkin lymphoma as a model to demonstrate a method we have established and optimized to separate FFPE samples into distinct tumor and nonmalignant populations. Using FFPE reactive tonsil sections, various approaches for antigen retrieval and labeling, and the effectiveness of flow cytometric sorting were tested...
August 7, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28782855/effect-of-maleic-acid-on-the-bond-strength-of-fibre-posts-to-root-dentine
#16
Fan Fan, Muhammad Ibrahim, Panpan Dai, Yixin Mao, Bing He, Gang Wu, Jianfeng Ma, Shengbin Huang
The aim of this study was to evaluate the effect of maleic acid (MA) on both the bond strength of fibre post to root dentine and smear layer removal after post space preparation. Sixty, single-canal premolars were endodontically treated and randomly assigned to four groups: group 1 [0.9% saline solution (control]); group 2 [2.5% sodium hypochlorite (NaOCl)]; group 3 [17% ethylenediaminetetraacetic acid (EDTA) followed by 2.5% NaOCl]; and group 4 (7% MA followed by 2.5% NaOCl). Self-adhesive resin cement was used to test the adhesion of a glass-fibre post to the root dentine through a micropush-out test...
August 7, 2017: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/28782616/ec-piii-a-novel-non-hemorrhagic-procoagulant-metalloproteinase-purification-and-characterization-from-indian-echis-carinatus-venom
#17
Manisha Choudhury, Kanve Nagaraj Suvilesh, Bannikuppe Sannanayak Vishwanath, Devadasan Velmurugan
Procoagulant snake venom toxins find extensive use as reagents in laboratory tests and diagnostic kits. In the present study we report a novel P-III class procoagulant SVMP, EC-PIII from Echis carinatus venom. EC-PIII was purified using a combination of gel-filtration and anion-exchange chromatography. It has a molecular mass of 110kDa and is a dimeric protein as determined by SDS-PAGE. DLS results show that the protein is homogenous and stable in solution. Peptide mass fingerprinting revealed that the peptides obtained show high homology to the other members of SVMP family...
August 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28782428/enhanced-removal-of-heavy-metals-by-zerovalent-iron-in-designed-magnetic-reactors
#18
Peng Fan, Xiao Jiang, Junlian Qiao, Lina Li
A magnetic propeller agitator and a magnetic reactor were designed to enhance the removal of heavy metals by zerovalent iron (ZVI) in comparison with the non-magnetic reactor. The weak magnetic field (WMF) applied in the magnetic propeller agitator or the magnetic reactor significantly improved the Cu(II)-EDTA removal by ZVI from 10% without WMF to 98% with WMF within 2.5 h at pHini 6.0. Simultaneous removal of Cu(II) and As(V) by ZVI was performed in the magnetic reactor. The results show that the pseudo-first-order rate constants of Cu(II) and As(V) removal by ZVI in the magnetic reactor were increased by 1...
August 7, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28781833/edta-induced-pseudothrombocytosis-and-citrate-induced-platelet-agglutination-in-a-patient-with-waldenstrom-macroglobulinemia
#19
Pia Bükmann Larsen, Jonas Vikeså, Lennart Friis-Hansen
Hematology analyzers sometimes generate spurious results. A patient had EDTA-induced pseudothrombocytosis and platelet agglutination in citrate blood samples. This case verifies that addition of 1% paraformaldehyde to the citrate tubes can prevent platelet clumping. Further, it illustrates the advantages of having access to more than one platelet count method.
August 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/28780638/discrimination-of-adsorbed-double-stranded-and-single-stranded-dna-molecules-on-surfaces-by-fluorescence-emission-spectroscopy-using-acridine-orange-dye
#20
E Hoory, J Budassi, E Pfeffer, N Cho, J Thalappillil, J Andersen, M Rafailovich, J Sokolov
A sensitive and straightforward method for discriminating between surface-adsorbed double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA), based on analysis of the fluorescence emission spectra of DNAs dyed with the metachromatic dye acridine orange, has been developed. Since the degree of discrimination between dsDNA and ssDNA is dependent on dye-base ratio (as has been shown in early studies of DNAs in solution), a specific, reproducible protocol for obtaining good ss-ds discrimination was needed. We studied the emission spectra for DNAs dyed in-situ on two different surfaces, polymethylmethacrylate and poly-l-lysine, using acridine orange solutions of varying concentrations in either 2-(N-morpholino)ethanesulfonic acid (MES) or Tris-Borate EDTA (TBE) buffers...
August 5, 2017: Journal of Fluorescence
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