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https://www.readbyqxmd.com/read/27933254/characterization-of-a-novel-hatching-enzyme-purified-from-starfish-asterina-pectinifera
#1
Ji Hoon Choi, Sang Moo Kim
Hatching enzyme is a protease which can degrade the membrane of egg. In this study, a hatching enzyme was purified from starfish (Asterina pectinifera) with 6.34 fold of purification rate, 5.04 % of yield, and 73.87 U/mg of specific activity. The molecular weight of starfish hatching enzyme was 86 kDa, which was reduced to 62 kDa after removal of N-linked oligosaccharides. The optimal pH and temperature of the hatching enzyme activity were pH 7.0 and 40 °C, respectively, while those of stability were pH 8 and 20 °C...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27930821/assessment-of-asymmetrical-dimethylarginine-metabolism-in-patients-with-critical-illness
#2
Rawia A Ghashut, Scott Blackwell, Sylvia Ryan, Laura Willox, Donald C McMillan, John Kinsella, Dinesh Talwar
BACKGROUND: Critically ill patients experience metabolic disorders including hypercatabolic state and hyperglycaemia and these are associated with poor outcome. Hyperglycaemia and asymmetric dimethylarginine (ADMA) are reported to have significant influences on endothelial dysfunction. The aim of the present study was to examine the relationship between plasma asymmetric dimethylarginine (ADMA) and related arginine metabolism in patients with critical illness. MATERIALS AND METHODS: Two venous blood samples (EDTA) (104 patients), on admission and follow up sample in the last day in ICU (died or discharge sample median 7, IQR 6-8, range 5-15)...
December 8, 2016: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/27929196/real-time-detection-and-imaging-of-copper-ii-in-cellular-mitochondria
#3
Hong Li, Ruilong Zhang, Chunxia Li, Bei Huang, Tingting Yu, Xiaodan Huang, Xuanjun Zhang, Fei Li, Hongping Zhou, Yupeng Tian
A triphenylamine-pyridine derivative, viz., a fluorescence probe was designed and synthesized. The probe displayed an "on-off-on" fluorescence switch, upon the sequential addition of Cu(2+) and ethylenediaminetetraacetic acid (EDTA). The detection mechanism was confirmed by using UV-vis, fluorescence and (1)H NMR titration spectra. Interestingly, the probe can accumulate in mitochondria, and thus detect Cu(2+) in real-time there, which indicated that probe L can be used to monitor intracellular mitochondrial Cu(2+) levels...
December 8, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27925159/lectin-complement-pathway-proteins-in-healthy-individuals
#4
Anne Troldborg, Annette Hansen, Søren W K Hansen, Jens Christian Jensenius, Kristian Stengaard-Pedersen, Steffen Thiel
Since the discovery of the lectin pathway of complement activation, numerous clinical cohorts have been examined for one or more of the proteins, with the intention of uncovering the functions of the proteins or with the aim of discovering new biomarkers or diagnostic tools. To unveil the abnormal, it is pivotal to know the normal. Our aim was to describe the concentrations of the eleven known proteins of the lectin pathway in serum and plasma and to uncover possible gender differences, age and diurnal variations, which must be taken into account for investigations in different cohorts...
December 7, 2016: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/27925061/molecular-detection-of-anaplasma-species-in-dogs-in-colombia
#5
Giovanni Vargas-Hernandez, Marcos Rogério André, Diana Maria Cendales, Keyla Carstens Marques de Sousa, Luiz Ricardo Gonçalves, Mariana Cristina Hoeppner Rondelli, Rosangela Zacarias Machado, Mirela Tinucci-Costa
Anaplasma platys and A. phagocytophilum are tick-borne pathogens that parasitize platelets and neutrophils, respectively, of humans and animals. The former is the etiological agent of canine cyclic thrombocytopenia, while the latter is that of canine granulocytic anaplasmosis. This work involved the detection and identification of Anaplasma species in blood samples from dogs in Colombia, using molecular techniques. Between December 2008 and April 2009, blood samples were drawn from the cephalic vein of 91 dogs in the central-western region of Colombia (cities of Bogota, Villavicencio and Bucaramanga) and stored in tubes containing EDTA...
December 1, 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/27924070/modified-methods-for-isolation-of-pancreatic-stellate-cells-from-human-and-rodent-pancreas
#6
Liangtao Zhao, Baobao Cai, Zipeng Lu, Lei Tian, Song Guo, Pengfei Wu, Dong Qian, Qingcheng Xu, Kuirong Jiang, Yi Miao
Primary cultures of pancreatic stellate cells (PSCs) remain an important basis for in vitro study. However, effective methods for isolating abundant PSCs are currently lacking. We report on a novel approach to isolating PSCs from normal rat pancreases and human pancreatic ductal adenocarcinoma (PDAC) tissue. After anaesthesia and laparotomy of the rat, a blunt cannula was inserted into the pancreatic duct through the anti-mesentery side of the duodenum, and the pancreas was slowly infused with an enzyme solution until all lobules were fully dispersed...
November 2016: Journal of Biomedical Research
https://www.readbyqxmd.com/read/27923709/digesta-retention-patterns-in-geese-anser-anser-and-turkeys-meleagris-gallopavo-and-deduced-function-of-avian-caeca
#7
Samuel Frei, Sylvia Ortmann, Michael Kreuzer, Jean-Michel Hatt, Marcus Clauss
Although it is well-known that retrograde transport of urine fills the caeca of birds with fluid and small particles, the function of avian caeca is still not fully understood. We measured mean retention times (MRT) of solute (cobalt-EDTA, Co), small particle (<2mm, chromium-mordanted fibre, Cr) and large particle (8mm, cerium-marked fibre, Ce) markers in geese (Anser anser) and turkeys (Meleagris gallopavo) fed alfalfa pellets ad libitum. Intake did not differ between species. Turkeys had longer MRT for all markers (Co: 10...
December 3, 2016: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/27921101/the-effect-of-common-anticoagulants-in-detection-and-quantification-of-malaria-parasitemia-in-human-red-blood-cells-by-atr-ftir-spectroscopy
#8
Miguela Martin, David Perez-Guaita, Dean W Andrew, Jack S Richards, Bayden R Wood, Philip Heraud
Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) has the potential to become a new diagnostic tool for malaria and other diseases. For point-of-care testing, the use of ATR-FTIR in malaria diagnosis enables the analysis of blood in the aqueous state, which represents an enormous advantage by minimising the sample preparation by removing the need for cell fixation. Here we report the quantification of malaria parasitemia in human RBCs in their normal physiological aqueous state...
December 6, 2016: Analyst
https://www.readbyqxmd.com/read/27920886/evaluation-of-recombinant-human-growth-hormone-secretion-in-e-coli-using-the-l-asparaginase-ii-signal-peptide
#9
Mozhdeh Zamani, Navid Nezafat, Younes Ghasemi
BACKGROUND: In the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. Signal peptides play a critical role in prosperous secretion of recombinant proteins. Accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-error experiments. The aim of this study was to evaluate the effect of L-asparaginase II signal peptide on the recombinant human Growth Hormone (hGH) protein secretion in the Escherichia coli (E...
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27920823/antioxidant-effects-of-hydroalcoholic-and-polyphenolic-extracts-of-peucedanum-pastinacifolium-boiss-hausskn
#10
Ahmad Movahedian, Behzad Zolfaghari, Mehrzad Mirshekari
Antioxidant activity of Peucedanum pastinacifolium Boiss. & Hausskn aerial part hydroalcoholic extract (HAE) and polyphenolic extract (PPE) as well as their total phenolic and flavonoid contents were studied. Phenolic and flavonoid contents were respectively estimated as gallic acid and quercetin equivalents. The in vitro antioxidant activity of two extracts of P. pastinacifolium were evaluated by radical scavenging of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), chelating activity on ferrous ions, or ferric reducing antioxidant power (FRAP) assay...
October 2016: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27919366/ldl-apheresis-activates-the-complement-system-and-the-cytokine-network-whereas-pcsk9-inhibition-with-evolocumab-induces-no-inflammatory-response
#11
Knut Tore Lappegård, Terje Enebakk, Hilde Thunhaug, Judith Krey Ludviksen, Tom Eirik Mollnes, Anders Hovland
BACKGROUND: Low-density lipoprotein (LDL) apheresis is an extracorporeal treatment modality used in high-risk coronary patients. It may, however, induce complement activation and downstream inflammation due to bio-incompatibility. OBJECTIVE: We explored changes in soluble inflammatory markers when changing from LDL apheresis to the novel PCSK9 inhibitor evolocumab. METHODS: Three patients with familial hypercholesterolemia participated. Blood samples (EDTA plasma) for complement activation and markers of inflammation were obtained before (baseline) and after LDL apheresis week at 0 and before biweekly administration of evolocumab at weeks 1, 3, 5, and 7...
November 2016: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/27917319/the-effectiveness-of-cooling-conditions-on-temperature-of-canine-edta-whole-blood-samples
#12
Karen M Tobias, Leslie Serrano, Xiaocun Sun, Bente Flatland
BACKGROUND: Preanalytic factors such as time and temperature can have significant effects on laboratory test results. For example, ammonium concentration will increase 31% in blood samples stored at room temperature for 30 min before centrifugation. To reduce preanalytic error, blood samples may be placed in precooled tubes and chilled on ice or in ice water baths; however, the effectiveness of these modalities in cooling blood samples has not been formally evaluated. The purpose of this study was to evaluate the effectiveness of various cooling modalities on reducing temperature of EDTA whole blood samples...
2016: PeerJ
https://www.readbyqxmd.com/read/27916492/comparative-impacts-of-iron-oxide-nanoparticles-and-ferric-ions-on-the-growth-of-citrus-maxima
#13
Jing Hu, Huiyuan Guo, Junli Li, Qiuliang Gan, Yunqiang Wang, Baoshan Xing
The impacts of iron oxide nanoparticles (γ-Fe2O3 NPs) and ferric ions (Fe(3+)) on plant growth and molecular responses associated with the transformation and transport of Fe(2+) were poorly understood. This study comprehensively compared and evaluated the physiological and molecular changes of Citrus maxima plants as affected by different levels of γ-Fe2O3 NPs and Fe(3+). We found that γ-Fe2O3 NPs could enter plant roots but no translocation from roots to shoots was observed. 20 mg/L γ-Fe2O3 NPs had no impact on plant growth...
December 1, 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27915165/re-assessing-gallium-67-as-a-therapeutic-radionuclide
#14
Muhamad F Bin Othman, Nabil R Mitry, Valerie J Lewington, Philip J Blower, Samantha Y A Terry
INTRODUCTION: Despite its desirable half-life and low energy Auger electrons that travel further than for other radionuclides, (67)Ga has been neglected as a therapeutic radionuclide. Here, (67)Ga is compared with Auger electron emitter (111)In as a potential therapeutic radionuclide. METHODS: Plasmid pBR322 studies allowed direct comparison between (67)Ga and (111)In (1MBq) in causing DNA damage, including the effect of chelators (EDTA and DTPA) and the effects of a free radical scavenger (DMSO)...
October 29, 2016: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/27913270/part-1-comparison-of-plasma-ammonia-results-from-seven-different-automated-platforms-in-use-throughout-central-australia
#15
Corey Markus, Michael Metz
INTRODUCTION: The clinical catchment area for the Metabolic service at the Women's and Children's Hospital in Adelaide, South Australia, covers nearly 2.5millionkm(2). Care of children with metabolic disorders in these remote areas is assisted from Adelaide, and at times, using plasma ammonia results from laboratories up to 3000km away. There are seven different platforms measuring plasma ammonia within this vast clinical catchment area. Hence, a correlation study was conducted to examine the relationship between plasma ammonia results from the seven different platforms in use throughout central Australia...
November 29, 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27904395/purification-and-characterization-of-thermostable-and-detergent-stable-%C3%AE-amylase-from-anoxybacillus-sp-ah1
#16
Ömer Acer, Fatma Matpan Bekler, Hemşe Pirinççioğlu, Reyhan Gül Güven, Kemal Güven
A thermostable and detergent-stable α-amylase from a newly isolated Anoxybacillus sp. AH1 was purified and characterized. Maximum enzyme production (1874.8 U/mL) was obtained at 24 h of incubation. The amylase was purified by using Sephadex G-75 gel filtration, after which an 18-fold increase in specific activity and a yield of 9% were achieved. The molecular mass of the purified enzyme was estimated at 85 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimum pH and temperature values of the enzyme were 7...
March 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27904087/push-out-bond-strength-of-a-new-post-system-after-various-post-space-treatments
#17
Melek Akman, Ayçe Unverdi Eldeniz, Selen Ince, Mehmet Burak Guneser
To assess the effect of post-space treatment with chelating agents on the push-out bond-strength of a glass fiber post-system. Fortyeight human teeth were decoronated. The roots were prepared to size 40 and obturated. The post-spaces were prepared with PeesoReamer drills. The post-spaces were irrigated with (i) NaOCl and EDTA with chlorhexidine (QMix), (ii) NaOCl and EDTA, (iii) NaOCl and Citric acid, (iv) NaOCl and saline as a control group. i-TFC glass fiber posts were then luted with i-TFC bond system. The samples were horizontally sectioned...
2016: Dental Materials Journal
https://www.readbyqxmd.com/read/27898046/pro-and-antioxidant-activity-of-three-selected-flavan-type-flavonoids-catechin-eriodictyol-and-taxifolin
#18
Vladimir Chobot, Franz Hadacek, Gert Bachmann, Wolfram Weckwerth, Lenka Kubicova
The flavanol (±)-catechin shows an OH group but no 4-keto group on ring C (C3), and no conjugation between ring A and B. The related flavanone (+)-eriodictyol has a keto group on C4 but no 3-OH group on ring C. (+)-Taxifolin, another flavanone, has an OH on C3 and a keto group on C4 of the C ring. Deoxyribose degradation assay systems, with hydrogen peroxide and ascorbic acid either added or omitted, were performed in variants in which Fe(III) was added in a complex with ethylenediaminetetraacetic acid (EDTA)...
November 26, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27896275/rationale-for-the-successful-management-of-edta-chelation-therapy-in-human-burden-by-toxic-metals
#19
REVIEW
Maria Elena Ferrero
Exposure to environmental and occupational toxicants is responsible for adverse effects on human health. Chelation therapy is the only procedure able to remove toxic metals from human organs and tissue, aiming to treat damage related to acute and/or chronic intoxication. The present review focuses on the most recent evidence of the successful use of the chelating agent ethylenediaminetetraacetic acid (EDTA). Assessment of toxic-metal presence in humans, as well as the rationale of EDTA therapy in cardiovascular and neurodegenerative diseases, is reported...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27893355/performance-evaluation-study-of-id-core-xt-a-high-throughput-blood-group-genotyping-platform
#20
Mónica López, Izaskun Apraiz, Montserrat Rubia, Mercedes Piedrabuena, Maria Azkarate, Barbera Veldhuisen, Miguel Á Vesga, Ellen Van Der Schoot, Fernando Puente, Diego Tejedor
BACKGROUND: Traditionally, red blood cell antigens have been identified using serological methods, but recent advances in molecular biology have made the implementation of methods for genetic testing of most blood group antigens possible. The goal of this study was to validate the performance of the ID CORE XT blood group typing assay. MATERIALS AND METHODS: One thousand independent samples from donors, patients and neonates were collected from three research institutes in Spain and the Netherlands...
November 25, 2016: Blood Transfusion, Trasfusione del Sangue
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