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https://www.readbyqxmd.com/read/29169185/co-culture-of-human-alveolar-epithelial-haelvi-and-macrophage-thp-1-cell-lines
#1
Stephanie Kletting, Sarah Barthold, Urska Repnik, Gareth Griffiths, Brigitta Loretz, Nicole Schneider-Daum, Cristiane de Souza Carvalho-Wodarz, Claus-Michael Lehr
The air-blood barrier is mainly composed of alveolar epithelial cells and macrophages. Whereas the epithelium acts as a diffusional barrier, macrophages represent an immunological barrier, in particular for larger molecules and nanoparticles. This paper describes a new co-culture of human cell lines representing both cell types. Acquiring, culturing and maintaining primary alveolar epithelial cells presents significant logistical and technical difficulties. The recently established human alveolar epithelial lentivirus immortalized cell line, hAELVi, when grown on permeable filters, form monolayers with high functional and morphological resemblance to alveolar type I cells...
November 23, 2017: ALTEX
https://www.readbyqxmd.com/read/29166020/solid-digestion-of-demineralized-bone-as-a-method-to-access-potentially-insoluble-proteins-and-post-translational-modifications
#2
Timothy P Cleland
Bone proteomics is an expanding field for understanding protein changes associated with disease as well as characterizing and detecting proteins preserved in fossil bone. Most previous studies have utilized a protocol with demineralization and extraction approach to isolate and characterize proteins from bone. Through near-complete EDTA demineralization, followed by solid digestion of the remaining bone pseudomorph, a total of 92 protein accessions were detected from dog bone. In the EDTA, 14 unique proteins were found, including osteocalcin, an important bone protein...
November 22, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29165807/arabidopsis-thaliana-plants-challenged-with-uranium-reveal-new-insights-into-iron-and-phosphate-homeostasis
#3
Serge Berthet, Florent Villiers, Claude Alban, Nelson B C Serre, Jacqueline Martin-Laffon, Sylvie Figuet, Anne-Marie Boisson, Richard Bligny, Marcel Kuntz, Giovanni Finazzi, Stéphane Ravanel, Jacques Bourguignon
Uranium (U) is a naturally occurring radionuclide that is toxic to plants. It is known to interfere with phosphate nutrition and to modify the expression of iron (Fe)-responsive genes. The transporters involved in the uptake of U from the environment are unknown. Here, we addressed whether IRT1, a high-affinity Fe(2+) transporter, could contribute to U uptake in Arabidopsis thaliana. An irt1 null mutant was grown hydroponically in different conditions of Fe bioavailability and phosphate supply, and challenged with uranyl...
November 22, 2017: New Phytologist
https://www.readbyqxmd.com/read/29164636/development-and-validation-of-a-simple-solid-phase-extraction-method-coupled-with-liquid-chromatography-triple-quadrupole-tandem-mass-spectrometry-for-simultaneous-determination-of-lincomycin-tylosin-a-and-tylosin-b-in-royal-jelly
#4
Weijia Zheng, Jin-A Park, A M Abd El-Aty, Seong-Kwan Kim, Sang-Hyun Cho, Jeong-Min Choi, Mohamad Warda, Jing Wang, Jae-Han Shim, Ho-Chul Shin
We develop an analytical method for the determination of lincomycin, tylosin A, and tylosin B residues in royal jelly using liquid chromatography-triple quadrupole tandem mass spectrometry analysis. For extraction and purification, we employ 1% trifluoroacetic acid and 0.1 M Na2 EDTA solutions along with an Oasis HLB cartridge. The target antibiotics were well separated in a Kinetex EVO C18 reversed-phase analytical column using a combination of 0.1% formate acid in ultrapure water (A) and acetonitrile (B) as the mobile phase...
November 22, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29162770/correlation-of-the-blood-test-results-obtained-between-assays-using-microliter-scale-fingertip-blood-samples-collected-with-a-novel-blood-collection-device-and-conventional-venous-blood-assays-a-secondary-publication-in-english
#5
Hajime Iwasawa, Tomoaki Nishimura, Shota Nemoto, Naoki Aikawa, Kiyoaki Watanabe
In this study, we investigated the correlations between biochemical and hematological test results obtained using microliter-scale fingertip blood samples collected with a newly developed blood collection device and those obtained using conventional venous blood. Eighty volunteer subjects were enrolled in this study. Blood samples were drawn from the fingertip of the ring finger by a single puncture, and 60-µL samples were promptly and accurately aspirated into a blood collection chip. Then the chip was tightly sealed in a chip container and was shaken to mix the contents without dispersion...
November 21, 2017: Keio Journal of Medicine
https://www.readbyqxmd.com/read/29162409/high-level-expression-and-characterization-of-tannase-tan7-using-aspergillus-niger-sh-2-with-low-background-endogenous-secretory-proteins-as-the-host
#6
Fengling Liu, Bin Wang, Yanrui Ye, Li Pan
Tannin acyl hydrolase (tannase, EC3.1.1.20) catalyzes the hydrolysis of hydrolyzable tannins. It is used in the manufacture of instant tea and in the production of gallic acid. In this study, we reported that the overexpression, purification and characterization of an Aspergillus niger tannase. The tannase gene was cloned from A.niger SH-2 and expressed in the A.niger strain Bdel4 which is low-background of secreted proteins. The recombinant tannase was purified by desalting, followed by gel filtration for characterization...
November 18, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29158701/study-of-dna-damage-caused-by-dipyrone-in-presence-of-some-transition-metal-ions
#7
Bruna Corrêa Roriz, Horacio Dorigan Moya
The DNA damage in the presence of dipyrone (used as its sodium salt, NaDip) and some transition metal ions in an air saturated ([O2] ≈ 0.25 mM) non-buffered solution at T = (25.0 ± 0.5)°C was investigated by agarose gel electrophoresis. As metal ions Cu(2+), Fe(3+), Ni(2+) and Mn(3+) were selected and evaluated in the present study because of the important role they play in a biological system. pUC19 plasmid DNA damage-induced by NaDip (80-600 μM) was observed in the presence of 100 μM Cu(2+)...
November 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/29158198/vitamin-c-exerts-novel-protective-effects-against-cadmium-toxicity-in-mouse-spermatozoa-by-inducing-the-dephosphorylation-of-dihydrolipoamide-dehydrogenase
#8
Lirui Wang, Peifei Li, Yi Wen, Qiangzhen Yang, Linqing Zhen, Jieli Fu, Yuhua Li, Sisi Li, Chengxiao Han, Xinhong Li
Cadmium (Cd) has been reported to inhibit mouse sperm motility by inducing the tyrosine phosphorylation of dihydrolipoamide dehydrogenase (DLD). This study aimed to assess the potential effects of vitamin C (Vc) on ameliorating Cd-induced tyrosine phosphorylation of DLD and the specific underlying mechanism. Vc induced the dephosphorylation of DLD or inhibited the tyrosine phosphorylation of DLD. Accordingly, DLD activity, nicotinamide adenine dinucleotide hydrogen (NADH) levels, ATP levels and motility parameters were all restored to normal levels by Vc...
November 20, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/29157956/comparative-analysis-of-fixation-and-embedding-techniques-for-optimized-histological-preparation-of-zebrafish
#9
Jean E Copper, Lynn R Budgeon, Christina A Foutz, Damian B van Rossum, Daniel J Vanselow, Margaret J Hubley, Darin P Clark, David T Mandrell, Keith C Cheng
In recognition of the importance of zebrafish as a model organism for studying human disease, we have created a web-based reference atlas of zebrafish microanatomy for comparing histology and histopathology between model systems and with humans (http://bio-atlas.psu.edu). Fixation, decalcification, embedding, and sectioning were optimized to maximize section quality. A comparison of protocols involving six fixatives showed that 10% Neutral Buffered Formalin at 21°C for 24h yielded excellent results. Sectioning of juveniles and adults requires bone decalcification; EDTA at 0...
November 17, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/29155127/mdr1-gene-c3435t-polymorphism-in-chronic-hepatitis-c-patients
#10
Mehdi Parsanahad, Manoochehr Makvandi, Ali Teimoori, Shahram Jalilian, Gholamabas Kayedani, Sara Mahmoodi
BACKGROUND: MDR1 is a highly polymorphic gene that encodes P-glycoprotein (P-gp). This protein anchor to the cell membrane and transports toxins, xenobiotic, chemicals, and drugs from the intracellular to extracellular and thus protect cells. Polymorphism of the MDR1 gene seems to be effective in gene expression and response to treatment. Since one of the main mechanisms of drug resistance is the removal of the drug from the cell by ATP-dependent efflux proteins, thus MDR1, single nucleotide polymorphism (SNP) C3435T can be used as a predictor for treatment outcomes...
November 15, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29154238/ctrc-gene-polymorphism-may-increase-pancreatic-cancer-risk-preliminary-study
#11
Stanisław Głuszek, Artur Kowalik, Dorota Kozieł, Iwona Wawrzycka, Martyna Głuszek-Osuch, Jarosław Matykiewicz
Pancreatic cancer is often fatal due to delayed diagnosis and treatment difficulties. OBJECTIVE: To analyze selected SPINK1, CTRC, CFTR, and PRSS1 gene mutations in cancer tissue and blood samples of patients with pancreatic tumors. MATERIALS AND METHOD: We enrolled 16 consecutive patients diagnosed with pancreatic tumors. We collected cancer tissue, normal pancreatic tissue, and blood samples for genetic tests. The control group consisted of 419 healthy individuals...
October 31, 2017: Polski Przeglad Chirurgiczny
https://www.readbyqxmd.com/read/29153329/efficient-removal-of-edta-complexed-cu-ii-by-a-combined-fe-iii-uv-alkaline-precipitation-process-performance-and-role-of-fe-ii
#12
Chao Shan, Zhe Xu, Xiaolin Zhang, You Xu, Guandao Gao, Bingcai Pan
Efficient removal of heavy metal-EDTA complexes from water remains a challenge because of their good solubility and chemical stability. Herein, we employed a proprietary process, i.e., the Fe(III) displacement/UV irradiation/alkaline precipitation (denoted as Fe(III)/UV/OH), to enable an efficient removal of Cu(II)-EDTA complex from 19.2 mg Cu(II)/L to <1 mg Cu(II)/L. The combined process includes Fe(III) replacement with the complexed Cu(II) to form Fe(III)-EDTA and release the free Cu(II), UV-mediated catalytic decarboxylation of EDTA to form amine ligands and reduction of Fe(III) to Fe(II), and the final removal of Cu(II) through precipitation...
October 25, 2017: Chemosphere
https://www.readbyqxmd.com/read/29152696/degradation-of-the-earthy-and-musty-odorant-2-4-6-tricholoroanisole-by-persulfate-activated-with-iron-of-different-valences
#13
Kejia Zhang, Xinyan Zhou, Tuqiao Zhang, Lang Yu, Zheming Qian, Wenchao Liao, Cong Li
2,4,6-Trichloroanisole (TCA) is an odorous compound that is often detected in tap water and is difficult to be removed via water treatment. In this study, the transformation efficiency of TCA in the presence of persulfate (PS) activated by iron (Fe(2+), Fe(0), and Fe(3+)) was investigated for the first time. The effects of the activator dosage, oxidant dosage, pH, dosing method, chelating agents, humic acid, and temperature were evaluated. The degradation rate of TCA increased with increasing PS dosages (0...
November 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29152135/evaluation-of-pre-analytical-conditions-and-comparison-of-the-performance-of-several-digital-pcr-assays-for-the-detection-of-major-egfr-mutations-in-circulating-dna-from-non-small-cell-lung-cancers-the-circan_0-study
#14
Jessica Garcia, Eric Dusserre, Valérie Cheynet, Pierre Paul Bringuier, Karen Brengle-Pesce, Anne-Sophie Wozny, Claire Rodriguez-Lafrasse, Gilles Freyer, Marie Brevet, Léa Payen, Sébastien Couraud
Non invasive somatic detection assays are suitable for repetitive tumor characterization or for detecting the appearance of somatic resistance during lung cancer. Molecular diagnosis based on circulating free DNA (cfDNA) offers the opportunity to track the genomic evolution of the tumor, and was chosen to assess the molecular profile of several EGFR alterations, including deletions in exon 19 (delEX19), the L858R substitution on exon 21 and the EGFR resistance mutation T790M on exon 20. Our study aimed at determining optimal pre-analytical conditions and EGFR mutation detection assays for analyzing cfDNA using the picoliter-droplet digital polymerase chain reaction (ddPCR) assay...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29151793/plasma-protein-absolute-quantification-by-nano-lc-q-tof-udms-e-for-clinical-biomarker-verification
#15
Maria Ilies, Cristina Adela Iuga, Felicia Loghin, Vishnu Mukund Dhople, Elke Hammer
Background and aims: Proteome-based biomarker studies are targeting proteins that could serve as diagnostic, prognosis, and prediction molecules. In the clinical routine, immunoassays are currently used for the absolute quantification of such biomarkers, with the major limitation that only one molecule can be targeted per assay. The aim of our study was to test a mass spectrometry based absolute quantification method for the verification of plasma protein sets which might serve as reliable biomarker panels for the clinical practice...
2017: Clujul Medical (1957)
https://www.readbyqxmd.com/read/29150635/one-pot-synthesis-of-trifunctional-chitosan-edta-%C3%AE-cyclodextrin-polymer-for-simultaneous-removal-of-metals-and-organic-micropollutants
#16
Feiping Zhao, Eveliina Repo, Dulin Yin, Li Chen, Simo Kalliola, Juntao Tang, Evgenia Iakovleva, Kam Chiu Tam, Mika Sillanpää
The global contamination of water resources with inorganic and organic micropollutants, such as metals and pharmaceuticals, poses a critical threat to the environment and human health. Herein, we report on a bio-derived chitosan-EDTA-β-cyclodextrin (CS-ED-CD) trifunctional adsorbent fabricated via a facile and green one-pot synthesis method using EDTA as a cross-linker, for the adsorption of toxic metals and organic micropollutants from wastewater. In this system, chitosan chain is considered as the backbone, and the immobilized cyclodextrin cavities capture the organic compounds via host-guest inclusion complexation, while EDTA-groups complex metals...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29149675/evaluation-of-potassium-ferrate-as-an-alternative-disinfectant-on-cyanobacteria-inactivation-and-associated-toxin-fate-in-various-waters
#17
Jiajia Fan, Bo-Hung Lin, Che-Wei Chang, Yuqing Zhang, Tsair-Fuh Lin
Potassium ferrate (K2FeO4) is an effective oxidant that may be used as a pre- or post-oxidant in the purification of source water with cyanobacterial issues. To provide a better basis for the application of this oxidant during water treatment processes, the impacts of K2FeO4 on the cell viability of Microcystis aeruginosa and the fate of associated microcystins (MCs) were investigated in various water matrices. The results showed that a water matrix can significantly affect the effectiveness of K2FeO4 on cyanobacteria inactivation...
November 9, 2017: Water Research
https://www.readbyqxmd.com/read/29148785/rapid-quantitative-measurements-of-paramagnetic-relaxation-enhancements-in-cu-ii-tagged-proteins-by-proton-detected-solid-state-nmr-spectroscopy
#18
Dwaipayan Mukhopadhyay, Philippe S Nadaud, Matthew D Shannon, Christopher P Jaroniec
We demonstrate rapid quantitative measurements of site-resolved paramagnetic relaxation enhancements (PREs), which are a source of valuable structural restraints corresponding to electron-nucleus distances in the ~10-20 Å regime, in solid-state nuclear magnetic resonance (NMR) spectra of proteins containing covalent Cu(2+)-binding tags. Specifically, using protein GB1 K28C-EDTA-Cu(2+) mutant as a model, we show the determination of backbone amide (15)N longitudinal and (1)H transverse PREs within a few hours of experiment time based on proton-detected 2D or 3D correlation spectra recorded with magic-angle spinning frequencies ≥ ~60 kHz for samples containing ~10-50 nanomoles of (2)H,(13)C,(15)N-labeled protein back-exchanged in H2O...
November 17, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29146282/-measurement-and-estimation-of-glomerular-filtration-rate
#19
Emmanuelle Vidal-Petiot, Martin Flamant
Glomerular filtration rate is the best quantitative marker of renal function. It allows diagnosis and classification of severe chronic kidney disease into five stages. Theoretically, the measurement of glomerular filtration rate is based on the calculation of urinary clearance of exogenous substances such as chromium 51-labeled inulin or ethylenediaminetetraacetic acid ([(51)Cr]-EDTA). In practice, glomerular filtration rate is most often estimated from equations derived from plasma creatinine. This article reviews the range of methods used to measure or approximate glomerular filtration rate and attempts to describe recent developments in this area, including those related to plasma creatinine assay methods, creatinine and cystatin C concentration-derived estimations...
November 13, 2017: Néphrologie & Thérapeutique
https://www.readbyqxmd.com/read/29145088/phosphorus-recovery-and-leaching-of-trace-elements-from-incinerated-sewage-sludge-ash-issa
#20
Le Fang, Jiang-Shan Li, Ming Zhi Guo, C R Cheeseman, Daniel C W Tsang, Shane Donatello, Chi Sun Poon
Chemical extraction of phosphorus (P) from incinerated sewage sludge ash (ISSA) is adversely influenced by co-dissolution of metals and metalloids. This study investigated P recovery and leaching of Zn, Cu, Pb, As and Ni from ISSA using inorganic acids (sulphuric acid and nitric acid), organic acids (oxalic acid and citric acid), and chelating agents (ethylenediaminetetraacetic acid (EDTA) and ethylene diamine tetramethylene phosphonate (EDTMP)). The aim of this study was to optimize a leaching process to recover P-leachate with high purity for P fertilizer production...
November 8, 2017: Chemosphere
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