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https://www.readbyqxmd.com/read/29778467/short-communication-effect-of-abomasal-inorganic-phosphorus-infusion-on-phosphorus-absorption-in-large-intestine-milk-production-and-phosphorus-excretion-of-dairy-cattle
#1
X Feng, P P Ray, J P Jarrett, L Karpinski, B Jones, K F Knowlton
The objective of the study was to evaluate the effect of inorganic phosphorus (Pi) infusion on P absorption in large intestine, milk production, and phosphorus excretion. Four ruminally and ileally cannulated crossbred cows were used in a 4 × 4 Latin square with 21-d periods. Cows were fed a total mixed ration containing 0.21% P, providing 50% of the cows' P requirement. Cobalt-EDTA was used as marker to measure large intestine digesta flow. On d 13 to 21 of each period, each cow was infused daily with 0, 20...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29778076/design-and-synthesis-of-core-shell-fe-3-o-4-ptmt-composite-magnetic-microspheres-for-adsorption-of-heavy-metals-from-high-salinity-wastewater
#2
Xin Huang, Jinyue Yang, Jingkang Wang, Jingtao Bi, Chuang Xie, Hongxun Hao
In this study, a novel magnetic nanoparticles (MNP) modified by an organodisulfide polymer (PTMT) was designed for adsorption of heavy metals (Hg(II), Pb(II) and Cd(II)) from simulated coal chemical high salinity wastewater. The MNP-PTMT nano-composite was synthesize and characterized by SEM, TEM, FTIR, BET, VSM, TGA and XRD. The results indicate that the wanted MNP-PTMT magnetic nanoparticles were successfully obtained by modification. Adsorption experiments were systematically carried out to evaluate the performance of the obtained nanoparticles and to build up the adsorption models...
May 10, 2018: Chemosphere
https://www.readbyqxmd.com/read/29777964/protection-of-gnrh-analogue-by-chitosan-dextran-sulfate-nanoparticles-for-intravaginal-application-in-rabbit-artificial-insemination
#3
L Casares-Crespo, P Fernández-Serrano, M P Viudes-de-Castro
The present study was designed to prove new rabbit insemination extenders containing aminopeptidase inhibitors (AMIs) with or without chitosan (CS)-dextran sulfate (DS) nanoparticles entrapping the GnRH analogue. In addition, different hormone concentrations were tested in these extenders, evaluating their in vivo effect on rabbit reproductive performance after artificial insemination. A total of 911 females were inseminated with semen diluted with the four experimental extenders (C4 group: 4 μg buserelin/doe in control medium (Tris-citric acid-glucose supplemented with bestatin 10 μM and EDTA 20 mM), C5 group: 5 μg of buserelin/doe in control medium, Q4 group: 4 μg of buserelin/doe into CS-DS nanoparticles in control medium, Q5 group: 5 μg of busereline/doe into CS-DS nanoparticles in control medium)...
May 16, 2018: Theriogenology
https://www.readbyqxmd.com/read/29777513/a-novel-malic-enzyme-gene-mime2-from-mortierella-isabellina-m6-22-contributes-to-lipid-accumulation
#4
Shan Li, Lingyan Li, Xiangfeng Xiong, Xiuling Ji, Yunlin Wei, Lianbing Lin, Qi Zhang
OBJECTIVE: This study was aimed at cloning and characterizing a novel malic enzyme (ME) gene of Mortierella isabellina M6-22 and identifying its relation with lipid accumulation. METHODS: Mime2 was cloned from strain M6-22. Plasmid pET32aMIME2 was constructed to express ME of MIME2 in Escherichia coli BL21. After purification, the optimal pH and temperature of MIME2, as well as Km and Vmax for NADP+ were determined. The effects of EDTA or metal ions (Mn2+ , Mg2+ , Co2+ , Cu2+ , Ca2+ , or Zn2+ ) on the enzymatic activity of MIME2 were evaluated...
May 18, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29775822/nanocomposite-thin-films-ag-0-np-tio-2-in-the-efficient-removal-of-micro-pollutants-from-aqueous-solutions-a-case-study-of-tetracycline-and-sulfamethoxazole-removal
#5
Alka Tiwari, Alok Shukla, Lalliansanga, Diwakar Tiwari, Seung Mok Lee
The aim of this communication is to synthesize novel Nanocomposite thin film materials (Ag0 (NP)/TiO2 ) using the template process. Surface morphology of materials was obtained by the Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) analyses. The presence of doped Ag-nanoparticles was confirmed by the TEM images along with the SEM-EDX analyses. The Atomic Force Microscopic images were demonstrated the surface roughness and thickness of Nanocomposite thin films. X-ray diffraction analysis confirmed that TiO2 was predominantly present to its anatase mineral phase...
May 15, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29774548/release-of-tgf-%C3%AE-1-into-root-canals-with-various-final-irrigants-in-regenerative-endodontics-an-in-vitro-analysis
#6
Y Chae, M Yang, J R Kim
AIM: To investigate the release of growth factors into the root canal space after various final irrigants during regenerative endodontic procedures. The residual cytotoxic effect of final irrigants on stem cells from the apical papilla (SCAP) was also examined. METHODOLOGY: To measure the release of TGF-β1, root segments (8mm long) were irrigated with 1.5% NaOCl followed by 20 mL of final irrigants; Saline, 17% EDTA, 10% citric acid, 10% or 37% phosphoric acid...
May 17, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29772299/biophenols-enzymes-%C3%AE-secretase-cholinesterases-histone-deacetylase-and-tyrosinase-inhibitors-from-olive-olea-europaea-l
#7
Syed Haris Omar, Christopher J Scott, Adam S Hamlin, Hassan K Obied
The focus of this study was on inhibition of enzymes involved in the pathogenesis Alzheimer's disease (AD) including prime amyloid beta (Aβ) producing enzyme (β-secretase: BACE-1) and disease progression enzymes including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), histone deacetylase (HDAC), and tyrosinase along with the catecholamine L-DOPA, by using olive biophenols. Here we report the strongest inhibition of BACE-1 from rutin (IC50 : 3.8 nM) followed by verbascoside (IC50 : 6.3 nM) and olive fruit extract (IC50 : 18 ng), respectively...
May 14, 2018: Fitoterapia
https://www.readbyqxmd.com/read/29767434/regenerative-surgical-therapy-for-peri-implantitis-using-deproteinized-bovine-bone-mineral-with-10-collagen-enamel-matrix-derivative-and-doxycycline-a-prospective-3-year-cohort-study
#8
Faustino Mercado, Stephen Hamlet, Saso Ivanovski
OBJECTIVES: There is limited evidence regarding the long-term efficacy of regenerative treatment for peri-implantitis. The aim of this study was to evaluate a combination therapy of deproteinized bovine bone mineral with 10% collagen (DBBMC), enamel matrix derivative (EMD) and Doxycycline in the regeneration of bone defects associated with peri-implantitis. METHODS: Thirty patients diagnosed with peri-implantitis (BoP/suppuration, probing depth greater than 4 mm, minimum radiographic bone loss of 20%, at least 2 years in function) were enrolled in the study...
May 16, 2018: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/29766364/binding-of-cd-ii-pb-ii-and-zn-ii-to-a-type-1-metallothionein-from-maize-zea-mays
#9
Lian Duan, Jing-Jing Kong, Tong-Qing Wang, Yue Sun
Metallothioneins (MTs) are a family of ubiquitous, low-molecular-mass, cysteine-rich proteins that play a significant role in maintaining intracellular metal homeostasis, eliminating metal toxification, and protecting cells against oxidative damages. Research activity on plant MTs, although known for 30 years, has only moderately increased in the past few years. In this study, a type 1 MT from maize (Zea mays) (ZmMT1) was successfully expressed in Escherichia coli strain BL21 (DE3). The UV absorption spectra recorded after the reconstitution of apo-ZmMT1 with different metals demonstrated that ZmMT1 can coordinate up to six Zn(II) ions, six Cd(II) ions, and even higher amounts of Pb(II)...
May 15, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29765286/reference-intervals-for-a-complete-blood-count-on-an-automated-haematology-analyser-sysmex-xn-in-healthy-adults-from-the-southern-metropolitan-area-of-barcelona
#10
Ariadna Arbiol-Roca, Claudia Elizabeth Imperiali, Magdalena Macià Montserrat, Ana Sancho Cerro, Anna Cortés Bosch de Basea, Lourdes Sánchez Navarro, Dolors Dot Bach, José Valero Politi
Background: The examination of peripheral blood is routinely used as a basic test in daily medical practice. Reliable reference intervals are necessary to avoid misdiagnoses, and the establishment of those intervals is an important task for clinical laboratories.The aim of the present study was to establish the reference intervals for complete blood count (CBC) on a Sysmex XN haematology analyser in healthy adults from the southern metropolitan area of Barcelona (Spain). Methods: A total of 213 apparently healthy adults who received a general health examination at Hospital Universitari de Bellvitge were recruited to this study...
April 2018: EJIFCC
https://www.readbyqxmd.com/read/29765079/determination-of-flumequine-enantiomers-and-7-hydroxyflumequine-in-water-and-sediment-by-chiral-hplc-coupled-with-hybrid-quadrupole-time-of-flight-mass-spectrometer
#11
Moyong Xue, Yuchang Qin, Xu Gu, Junguo Li, Yunfeng Gao, Xiaowei Yang, Ting Yao, Zhen Zhao
A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for simultaneous enantiomeric analysis of flumequine and its metabolite 7-hydroxyflumequine in water and sediment had been developed based on the separation method. Sediment samples were extracted with ACN and EDTA-Mcllvaine buffer solution (40:60, v/v) then were enriched and cleaned-up by Cleanert PEP solid-phase extraction cartridges. The extract solvent, solid cartridges, mobile phase ratios, and chiral separation column were all optimized to reach high sensitivity and selectivity, good peak shape, and satisfactory resolution...
May 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29763971/evaluation-of-the-effects-of-the-solution-used-for-electrochemical-dissolution-of-nickel-titanium-endodontic-files-on-dentine-structure-microhardness-and-cell-viability
#12
C C F Amaral, F Ormiga, L C Boldrini, P G Miranda, T A Mendonça, J M Granjeiro, J A C P Gomes
AIM: To evaluate the effects of the [NaF 12 g/L + NaCl 1 g/L] solution used in the electrochemical dissolution process of fractured endodontic files, as well as its NiTi-containing product, on dentine hardness, topography and human fibroblast viability. METHODOLOGY: Sixty single-rooted human teeth were evaluated for dentine microhardness using the Vickers hardness test and the area and number of dentinal tubules by scanning electron microscopy. The samples were divided according to the dentine surface treatment: distilled water; 17% EDTA; [NaF 12 g/L + NaCl 1 g/L]; and 17% EDTA + [NaF 12 g/L + NaCl 1 g/L]...
May 15, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29756774/selection-identification-and-binding-mechanism-studies-of-an-ssdna-aptamer-targeted-to-different-stages-of-e-coli-o157-h7
#13
Ying Zou, Nuo Duan, Shijia Wu, Mofei Shen, Zhouping Wang
Enterohemorrhagic Escherichia coli O157:H7 (E.coli O157:H7) is known as an important food-borne pathogens related to public health. In this study, aptamers which could bind to different stages of E.coli O157:H7 (adjustment phase, log phase and stationary phase) with high affinity and specificity was obtained by Whole Cell-SELEX method through fourteen selection rounds including three counter-selection rounds. Altogether, thirty-two sequences were obtained and nine families were classified to select the optimal aptamer...
May 14, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29755509/synthesis-structural-characterization-and-antibacterial-activity-of-novel-erbium-iii-complex-containing-antimony
#14
Ting Liu, Rong-Gui Yang, Guo-Qing Zhong
The novel 3D edta-linked heterometallic complex [Sb2 Er(edta)2 (H2 O)4 ]NO3 ·4H2 O (H4 edta = ethylenediaminetetraacetic acid) was synthesized and characterized by elemental analyses, single-crystal X-ray diffraction, powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and thermal analysis. The complex crystallizes in the monoclinic system with space group Pm . In the complex, each erbium(III) ion is connected with antimony(III) ions bridging by four carboxylic oxygen atoms, and in each [Sb(edta)]- anion, the antimony(III) ion is hexacoordinated by two nitrogen atoms and four oxygen atoms from the edta4- ions, together with a lone electron pair at the equatorial position...
2018: Bioinorganic Chemistry and Applications
https://www.readbyqxmd.com/read/29754312/cloning-purification-and-biochemical-characterisation-of-a-gh35-beta-1-3-beta-1-6-galactosidase-from-the-mucin-degrading-gut-bacterium-akkermansia-muciniphila
#15
Bi-Shan Guo, Feng Zheng, Lucy Crouch, Zhi-Peng Cai, Meng Wang, David N Bolam, Li Liu, Josef Voglmeir
A putative GH35 β-galactosidase gene from the mucin-degrading bacterium Akkermansia muciniphila was successfully cloned and further investigated. The recombinant enzyme with the molecular mass of 74 kDa was purified to homogeneity and biochemically characterised. The optimum temperature of the enzyme was 42 °C, and the optimum pH was determined to be pH 3.5. The addition of sodium dodecyl sulphate (SDS) reduced the enzyme's activity significantly. The addition of Mg2+ -ions decreased the activity of the β-galactosidase, whereas other metal ions or EDTA showed no inhibitory effect...
May 12, 2018: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29752162/synthesis-and-characterization-of-a-new-methacrylate-monomer-derived-from-the-cashew-nut-shell-liquid-cnsl-and-its-effect-on-dentinal-tubular-occlusion
#16
Madiana Magalhães Moreira, Lucas Renan Rocha da Silva, Talita Arrais Daniel Mendes, Sérgio Lima Santiago, Selma Elaine Mazzetto, Diego Lomonaco, Victor Pinheiro Feitosa
OBJECTIVE: The aim of this study was to synthesize, to characterize and to evaluate the effects on tubular occlusion of new monomer derived from cashew nut shell liquid (CNSL), also studying the effects of acid challenge (AC) on dentin surfaces treated with desensitizers. METHODS: The intermediary cardanol-epoxy (CNE) was synthesized through epoxidation of CNSL, followed by synthesis of cardanol-methacrylate-epoxy (CNME) through methacryloyl chloride esterification...
May 8, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29752142/genetic-diversity-of-theileria-equi-and-babesia-caballi-infecting-horses-of-central-southern-italy-and-preliminary-results-of-its-correlation-with-clinical-and-serological-status
#17
Giuseppe Manna, Antonella Cersini, Roberto Nardini, Leticia Elisa Bartolomé Del Pino, Valeria Antognetti, Maurizio Zini, Raffaella Conti, Raniero Lorenzetti, Vincenzo Veneziano, Gian Luca Autorino, Maria Teresa Scicluna
Babesia caballi and Theileria equi are tick-borne pathogens causing equine piroplasmosis infecting the Equidae family in which they cause significant sanitary and economic losses. Furthermore, equine piroplasmosis is included in the World Animal Health Organization (OIE) notifiable diseases list with possible movement restrictions for positive horses. Thirty-nine EDTA and whole-blood samples collected during 2013 and 2014 from symptomatic and asymptomatic horses of Central-Southern Italy were included in the present study either because of their strongly positive results in Real Time (RT) PCRs targeting the 18S rRNA gene specific for each piroplasm and/or due to their serological ELISA/18S rRNA RT-PCR discordant results...
May 4, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29751221/postmortem-role-of-calpain-11-in-ostrich-skeletal-muscle
#18
Ya-Shiou Chang, Man-Jung Hsu, Rong-Ghi R Chou
The postmortem calpain-11 role in ostrich muscle was investigated. Pairs of ostrich muscle (Iliotibialis cranialis) were excised from 32 ostrich carcasses in 3-h postmortem and randomly assigned into four treatments. The muscle was cut into 2.5-cm thick meat cores. The cores were incubated in 30 mM CaCl2 , 30 mM EDTA, 90 mM NaCl, or control. The cores from the left-side carcasses were sampled after 0, 1, 2, and 3 days of incubation at 5 °C, while the right-side meat cores were taken at 1-day and 3-day incubation for shear force measurements...
April 24, 2018: Meat Science
https://www.readbyqxmd.com/read/29750937/the-potential-role-of-a-turbidimetric-heart-type-fatty-acid-binding-protein-assay-to-aid-in-the-interpretation-of-persistently-elevated-non-changing-cardiac-troponin-i-concentrations
#19
Peter A Kavsak, Craig Ainsworth, Donald M Arnold, Terry Scott, Lorna Clark, Josko Ivica, Katharine Mackett, Richard Whitlock, Andrew Worster
BACKGROUND: Elevated and non-changing high-sensitivity cardiac troponin (hs-cTn) concentrations may suggest a process other than acute injury, possibly due to chronic condition(s) causing the elevation, an analytical error/interference or the formation of macrocomplexes. Heart-type fatty acid binding protein (H-FABP) might be useful in this setting to identify the etiology of abnormally high and non-changing cTn concentrations which could aid clinical decision making in the hospital setting...
May 8, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29750642/trueness-precision-and-stability-of-the-liaison-1-84-parathyroid-hormone-pth-third-generation-assay-comparison-to-existing-intact-pth-assays
#20
Andre Valcour, Claudia Zierold, Frank A Blocki, Douglas M Hawkins, Kevin J Martin, Sudhaker D Rao, Fabrizio Bonelli
BACKGROUND: Over the past few decades, parathyroid hormone (PTH) immunoassays have progressed through successive generations resulting in increased specificity and accuracy for detecting circulating PTH. With the introduction of third-generation assays, in which the biologically active PTH(1-84) is specifically targeted, the PTH(7-84) and other fragments are not detected. The specific recognition of only PTH(1-84) whole molecule allows for more reliable standardization and calibration than with the existing assays...
May 11, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
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