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https://www.readbyqxmd.com/read/28544621/a-cell-adhesive-peptide-from-tropoelastin-promotes-sequential-cell-attachment-and-spreading-via-distinct-receptors
#1
Pearl Lee, Giselle C Yeo, Anthony S Weiss
Tropoelastin is the dominant monomer that assembles to form elastin, which confers elasticity to vertebrate elastic tissues including skin, arteries and lungs. Tropoelastin interacts with cells through cell surface receptors including integrins and glycosaminoglycans (GAGs). As the region 17-18 is recognized as a key region in cell attachment and spreading, we utilized C-terminal truncated tropoelastin constructs containing dissected sections of domain 18. We mapped a cell interactive sequence of tropoelastin to domain 17 and the first 6 amino acids (aa) of domain 18...
May 24, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28543092/effect-of-ultrasonic-and-sonic-activation-of-root-canal-sealers-on-the-push-out-bond-strength-and-interfacial-adaptation-to-root-canal-dentine
#2
P E B Wiesse, R D Pereira, Y C T Silva-Sousa, C Estrela, M D Sousa-Neto, J D Pécora
AIM: To evaluate the effect of ultrasonic and sonic activation of two root canal sealers on interfacial adaptation and push-out bond strength (BS) to root canal dentine. METHODOLOGY: The root canals of seventy-eight roots of maxillary canines was prepared with Ni-Ti rotary instruments under irrigation with 2.5% NaOCl and 17% EDTA. Canals were filled by lateral condensation of gutta-percha and AH Plus (n=39) or MTA Fillapex (n=39). Three subgroups (n=13) were formed according to sealer activation: no activation (NA), sonic activation (SA, 20 s) and ultrasonic activation (US, 20 s)...
May 22, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28540842/reason-and-resolution-of-high-negative-control-beads-in-solid-phase-immunoassay
#3
Asmae Zerrouki, Sanae Ouadghiri, Nadia Benseffaj, Rachid Razine, Malika Essakalli
OBJECTIVES: The Luminex technology is the most sensitive diagnostic method for HLA antibody detection and identification. However, the interpretation of immunoassays is commonly affected by the artifact, and non-specific background. Sera from some patients show high negative control bead (NC) value, which makes assessing and interpretation of HLA antibodies difficult. In this study, we evaluated the effect of Adsorb Out reagent, dithiothreitol (DTT), and Ethylenediaminetetraacetic acid (EDTA) on the NC median fluorescence intensity value by comparing treated versus untreated patient sera...
May 22, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28539937/chlorhexidine-prevents-root-dentine-mineral-loss-and-fracture-caused-by-calcium-hydroxide-over-time
#4
Michael Ranniery Garcia Ribeiro, Érika Bárbara Abreu Fonseca Thomaz, Darlon Martins Lima, Tarcísio Jorge Leitão, José Bauer, Soraia De Fátima Carvalho Souza
Purpose. To evaluate the mineral ion loss of root dentine after treatment with 2% chlorhexidine solution (CHX) and to compare its yield and flexural strength (fs) after exposure to calcium hydroxide [Ca(OH)2]. Materials and Methods. Dentine bars (DB) were made from 90 roots of bovine incisors and randomized into three groups: GControl: distilled/deionized water (DDW), GNaOCl: 2.5% sodium hypochlorite + 17% EDTA, and GCHX: CHX + DDW. The release of phosphate (PO4) and calcium (Ca) ions was measured by spectrophotometry...
2017: International Journal of Dentistry
https://www.readbyqxmd.com/read/28539708/assessment-of-mexican-arnica-heterotheca-inuloides-cass-and-rosemary-rosmarinus-officinalis-extracts-on-dopamine-and-selected-biomarkers-of-oxidative-stress-in-stomach-and-brain-of-salmonella-typhimurium-infected-rats
#5
David Calderón Guzmàn, Maribel Ortiz Herrera, Norma Osnaya Brizuela, Gerardo Barragàn Mejía, Ernestina Hernàndez García, Hugo Juàrez Olguín, Armando Valenzuela Peraza, Norma Labra Ruíz, Daniel Santamaría Del Angel
BACKGROUND: The effects of some natural products on dopamine (DA) and 5-hydroxyindole acetic acid (5-HIAA) in brain of infected models are still unclear. OBJECTIVE: The purpose of this study was to measure the effect of Mexican arnica/rosemary (MAR) water extract and oseltamivir on both biogenic amines and some oxidative biomarkers in the brain and stomach of young rats under infection condition. METHODS: Female Wistar rats (weight 80 g) in the presence of MAR or absence (no-MAR) were treated as follows: group 1, buffer solution (controls); oseltamivir (100 mg/kg), group 2; culture of Salmonella typhimurium (S...
April 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28537029/arsenic-accumulation-and-physiological-attributes-of-spinach-in-the-presence-of-amendments-an-implication-to-reduce-health-risk
#6
Muhammad Shahid, Marina Rafiq, Nabeel Khan Niazi, Camille Dumat, Saliha Shamshad, Sana Khalid, Irshad Bibi
The current study examined the effect of calcium (Ca) and ethylenediaminetetraacetic acid (EDTA) on arsenic (As) uptake and toxicity to spinach (Spinacia oleracea) as well as assessed the potential human health risks. Spinach seedlings were exposed to three levels of As (25, 125, and 250 μM) alone or together with three levels of EDTA (25, 125, and 250 μM) and Ca (1, 5, and 10 mM). The effect of EDTA and Ca was assessed in terms of As contents in roots and shoots, hydrogen peroxide production, chlorophyll contents, and lipid peroxidation...
May 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28535879/characterization-of-a-new-candidate-isotopic-reference-material-for-natural-pb-using-primary-measurement-method
#7
Naoko Nonose, Toshihiro Suzuki, Ki-Cheol Shin, Tsutomu Miura, Akiharu Hioki
A lead isotopic standard solution with natural abundance has been developed by applying a mixture of a solution of enriched (208)Pb and a solution of enriched (204)Pb ((208)Pb-(204)Pb double spike solution) as bracketing method. The amount-of-substance ratio of (208)Pb:(204)Pb in this solution is accurately measured by applying EDTA titrimetry, which is one of the primary measurement methods, to each enriched Pb isotope solution. Also metal impurities affecting EDTA titration and minor lead isotopes contained in each enriched Pb isotope solution are quantified by ICP-SF-MS...
June 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28535524/genetics-and-autosomal-dominant-polycystic-kidney-disease-progression
#8
Valentina Corradi, Anna Giuliani, Fiorella Gastaldon, Massimo de Cal, Barbara Mancini, Anna Montaldi, Alberta Alghisi, Irene Capelli, Gaetano La Manna, Claudio Ronco
Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary kidney disease, accounting for 10% of European patients on renal replacement therapy. In the previous years, many approaches to slow the progression of ADPKD were studied and many clinical trials published. In addition to having diagnostic role, the description of the genotype is even important to predict the progression of the disease and contributes, combined with several other factors, to a more precise patients classification...
2017: Contributions to Nephrology
https://www.readbyqxmd.com/read/28535395/a-zinc-chloride-doped-adhesive-facilitates-sealing-at-the-dentin-interface-a-confocal-laser-microscopy-study
#9
Manuel Toledano, Raquel Osorio, Estrella Osorio, Inmaculada Cabello, Manuel Toledano-Osorio, Fátima S Aguilera
The aim of this study was to ascertain the effect of Zn-doping of dental adhesives and mechanical load cycling on the micromorphology of the resin-dentin interdiffusion zone (of sound and caries affected dentin). The investigation considered two different Zn-doped adhesive approaches and evaluated the interface using a doubled dye fluorescent technique and a calcium chelator fluorophore under a confocal laser scanning microscopy. Sound and carious dentin-resin interfaces of unloaded specimens were deficiently resin-hybridized, in general...
May 19, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28527949/microproteome-of-dentoalveolar-tissues
#10
Cristiane R Salmon, Ana Paula O Giorgetti, Adriana F Paes Leme, Romênia R Domingues, Tamara N Kolli, Brian L Foster, Francisco H Nociti
Proteomic analysis of extracellular matrices (ECM) of dentoalveolar tissues can provide insights into developmental, pathological, and reparative processes. However, targeted dissection of mineralized tissues, dental cementum (DC), alveolar bone (AB), and dentin (DE), presents technical difficulties. We demonstrate an approach combining EDTA decalcification and laser capture microdissection (LCM), followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), to analyze proteome profiles of these tissues...
May 17, 2017: Bone
https://www.readbyqxmd.com/read/28527844/evaluation-of-various-pretreatments-to-fiber-post-on-the-push-out-bond-strength-of-root-canal-dentin
#11
Ömer Kırmalı, Özlem Üstün, Alper Kapdan, Alper Kuştarcı
INTRODUCTION: The aim of this in vitro study was to compare the effect of different pretreatments (fiber post) with the laser-activated irrigation (LAI) technique (for removal of the smear layer) on root canal dentin in terms of push-out bond strength (PBS) in a fiber post. METHODS: Fifty freshly extracted mandibular single-rooted premolars were prepared, and LAI was applied to all roots (17% EDTA was 5 mL for 120 seconds with an erbium, chromium:yttrium-scandium-gallium-garnet laser [0...
May 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28526203/quantitative-evaluation-of-inhibitory-effect-of-various-substances-on-anaerobic-ammonia-oxidation-anammox
#12
Tomotaka Nakamura, Yuhki Harigaya, Yuya Kimura, Megumi Kuroiwa, Yuhri Kurata, Kazuichi Isaka, Yuichi Suwa
The inhibitory effect of 20 substances of various chemical species on the anaerobic ammonia oxidation (anammox) activity of an enrichment culture, predominated by Candidatus Brocadia, was determined systematically by using a (15)N tracer technique. The initial anammox rate was determined during first 25 min with a small-scale anaerobic batch incubation supplemented with possible inhibitors. Although Cu(2+) and Mn(2+) did not inhibit anammox, the remaining 18 substances [Ni(2+), Zn(2+), Co(2+), [Formula: see text] , Fe(2+), 4 amines, ethylenediaminetetraacetic acid (EDTA), ethylenediamine-N,N'-bis (2-hydroxyphenylacetic acid) (EDDHA), citric acid, nitrilotriacetic acid (NTA), N,N-dimethylacetamide (DMA), 1,4-dioxane, dimethyl sulfoxide (DMSO), N,N-dimethylformamide (DMF) and tetrahydrofuran (THF)] were inhibitory...
May 16, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28523047/alpha-thalassemia-deletions-found-in-suspected-cases-of-beta-thalassemia-major-in-pakistani-population
#13
Saba Shahid, Muhammad Nadeem, Danish Zahid, Jawad Hassan, Saqib Ansari, Tahir Shamsi
BACKGROUND & OBJECTIVE: Alpha (α) thalassemia is a hereditary disorder and is caused by deletions or mutations in globin genes. It is present in two clinically significant forms: hemoglobin Bart hydrops fetalis (Hb Bart) syndrome and hemoglobin H (HbH) disease. It is highly prevalent in South-East Asia or Mediterranean countries. The most common deletion reported in alpha thalassemia in Pakistani population was -α(3.7) with a frequency of 8.3%, and the rare forms were -α(4.2) (0.2%) and ααα(anti3...
March 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28518207/influence-of-particle-size-and-dielectric-environment-on-the-dispersion-behaviour-and-surface-plasmon-in-nickel-nanoparticles
#14
Vikash Sharma, Chanderbhan Chotia, Tarachand, Vedachalaiyer Ganesan, Gunadhor S Okram
Nickel nanoparticles (NPs) are promising candidates for various applications, including biomedical ones, as they have good magnetic properties as well as high thermal conductivity. We used well-characterized Ni NPs of average Scherrer sizes from 1.31 nm to 22.23 nm and investigated the effects of the primary particle size, size distribution and dielectric environments, and of separately adding non-ionic polyvinylpyrrolidone (PVP), cationic cetyltrimethylammonium bromide (CTAB) and anionic ethylenediaminetetraacetic acid (EDTA) in ethanol, on their stability and agglomeration behaviour using atomic force microscopy (AFM), particle size analysis and zeta potential study through dynamic light scattering (DLS) combined with UV-visible spectroscopy data...
May 18, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28516900/facile-preparation-of-protonated-hexaniobate-nanosheets-and-its-enhanced-photocatalytic-activity
#15
Xiaobin Liu, Wenxiu Que, Peng Chen, Yapeng Tian, Jie Liu, Zuoli He, Huanfu Zhou, Ling Bing Kong
Exfoliated hexaniobate nanosheets E-H2K2Nb6O17-x (E-HKNO) with broad light absorption (up to 850 nm) and high adsorption properties were prepared via ion exchange and transient annealing processes with micron-size K4Nb6O17 powders as the precursor. The as-prepared E-HKNO nanosheets show excellent visible light photodegradation performances when compared to degussa P25, which was evaluated in terms of degradation of Rhodamine B (Rh B). High adsorption and broad light absorption characteristics could be attributed to the exfoliation behavior and the reduction of surface Nb(5+) to Nb(4+), which was confirmed by x-ray photoelectron spectroscopy (XPS) and Raman spectra...
May 18, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28513789/effect-of-final-irrigation-protocols-on-microhardness-reduction-and-erosion-of-root-canal-dentin
#16
Flávia Emi Razera Baldasso, Luana Roleto, Vinicius Duval da Silva, Renata Dornelles Morgental, Patrícia Maria Poli Kopper
This study aimed to evaluate the effect of final irrigation protocols on microhardness reduction and erosion of root canal dentin. Sixty root canals from mandibular incisors were instrumented and randomly divided into six groups (n = 10) according to the irrigant used: QMiX, 17% EDTA, 10% citric acid (CA), 1% peracetic acid (PA), 2.5% NaOCl (solution control), and distilled water (negative control). The chelating solutions were used to irrigate the canal followed by 2.5% NaOCl as a final flush. After the irrigation protocols, all specimens were rinsed with 10 mL of distilled water to remove any residue of the chemical solutions...
May 15, 2017: Brazilian Oral Research
https://www.readbyqxmd.com/read/28513744/high-affinity-recognition-of-the-human-c-reactive-protein-independent-of-phosphocholine
#17
Jie Yang, Anna-Lena Gustavsson, Martin Haraldsson, Göran Karlsson, Thomas Norberg, Lars Baltzer
A high-affinity polypeptide conjugate 4-C25L22-DQ, has been developed for the molecular recognition of the human C-reactive protein, CRP, a well-known inflammation biomarker. CRP is one of the most frequently quantified targets in diagnostic applications and a target in drug development. With the exception of antibodies, most molecular constructs take advantage of the known affinity for CRP of phosphocholine that depends on Ca(2+) for its ability to bind. 4-C25L22-DQ which is unrelated to phosphocholine binds in the absence of Ca(2+) with a dissociation constant of 760 nM, an order of magnitude lower than that of phosphocholine, the KD of which is 5 μM...
May 17, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28513077/tuneable-luminescence-properties-of-edta-assisted-zns-mn-nanocrystals-from-a-yellow-orange-to-a-red-emission-band
#18
R Viswanath, H S Bhojya Naik, G Arun Kumar, I K Suresh Gowda, S Yallappa
Luminescence technology has been improved with the help of semiconductor nanoparticles that possess novel optical and electrical properties compared with their bulk counterpart. The aim of this study was to design semiconductor nanocrystals in their pure (ZnS) or doped form (ZnS:Mn) with different concentrations of Mn(2+) ions by a wet chemical route stabilized by ethylenediamine tetra-acetic acid (EDTA) and to evaluate their luminescence properties. The nanocrystals were characterized by physicochemical techniques such as X-ray diffraction (XRD), High-resolution transmission electron microscopy (HRTEM), selected area electron diffraction (SEAD), EDS, and ultraviolet (UV)-visible light and photoluminescence (PL) studies...
May 16, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28512483/efficacy-of-different-root-canal-irrigants-on-smear-layer-removal-after-post-space-preparation-a-scanning-electron-microscopy-evaluation
#19
Rahele Mirseifinejad, Mehdi Tabrizizade, Abdolrahim Davari, Fateme Mehravar
INTRODUCTION: Effective durable adhesion between post material and dentine using resin cements is essential for longevity of restoration. The aim of this in vitro study was to compare the effect of different irrigants on smear layer removal after post space preparation. METHODS AND MATERIALS: A total of 75 extracted anterior human teeth were selected. The canals were instrumented by rotary system and then were filled. After preparing the post space, teeth were divided into 5 groups according to irrigants: 17% EDTA; 17% EDTA+2% CHX; 5...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28511387/erythrocyte-alloimmunization-and-autoimmunization-among-blood-donors-and-recipients-visiting-a-tertiary-care-hospital
#20
Daljit Kaur, Lovenish Bains, Manoj Kandwal, Indu Parmar
INTRODUCTION: The ultimate aim of pretransfusion testing is the acceptable survival of donor red cells in recipient's body and antibody detection plays a critical role in achieving the same. The cornerstone of antibody detection method is detecting an unexpected antibody as against the expected antibodies of ABO blood group system. Autoantibodies can also interfere with the detection of clinically significant alloantibodies. AIM: To study the frequency of alloantibodies and autoantibodies in the healthy blood donors and patient population visiting our hospital...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
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