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https://www.readbyqxmd.com/read/28531652/lessons-learned-using-a-values-engaged-approach-to-attend-to-culture-diversity-and-equity-in-a-stem-program-evaluation
#1
Ayesha S Boyce
Evaluation must attend meaningfully and respectfully to issues of culture, race, diversity, power, and equity. This attention is especially critical within the evaluation of science, technology, engineering, and mathematics (STEM) educational programming, which has an explicit agenda of broadening participation. The purpose of this article is to report lessons learned from the implementation of a values-engaged, educative (Greene et al., 2006) evaluation within a multi-year STEM education program setting. This meta-evaluation employed a case study design using data from evaluator weekly systematic reflections, review of evaluation and program artifacts, stakeholder interviews, and peer review and assessment...
May 13, 2017: Evaluation and Program Planning
https://www.readbyqxmd.com/read/28531134/improving-video-segmentation-by-fusing-depth-cues-and-the-visual-background-extractor-vibe-algorithm
#2
Xiaoqin Zhou, Xiaofeng Liu, Aimin Jiang, Bin Yan, Chenguang Yang
Depth-sensing technology has led to broad applications of inexpensive depth cameras that can capture human motion and scenes in three-dimensional space. Background subtraction algorithms can be improved by fusing color and depth cues, thereby allowing many issues encountered in classical color segmentation to be solved. In this paper, we propose a new fusion method that combines depth and color information for foreground segmentation based on an advanced color-based algorithm. First, a background model and a depth model are developed...
May 21, 2017: Sensors
https://www.readbyqxmd.com/read/28526665/the-sensor-study-protocol-for-a-mixed-methods-study-of-self-management-checks-to-predict-exacerbations-of-pseudomonas-aeruginosa-in-patients-with-long-term-respiratory-conditions
#3
Claire Roberts, Thomas L Jones, Samal Gunatilake, Will Storrar, Scott Elliott, Sharon Glaysher, Ben Green, Steven Rule, Carole Fogg, Ann Dewey, Kevin A Auton, Anoop J Chauhan
BACKGROUND: There are an estimated three million people in the United Kingdom with chronic obstructive pulmonary disease (COPD), and the incidence of bronchiectasis is estimated at around 0.1% but is more common in COPD and severe asthma. Both COPD and bronchiectasis are characterized by exacerbations in which bacteria play a central role. Pseudomonas aeruginosa is isolated from sputum samples from 4% to 15% of adults with COPD and is more likely to be isolated from patients with severe disease...
May 19, 2017: JMIR Research Protocols
https://www.readbyqxmd.com/read/28523919/editing-plants-for-virus-resistance-using-crispr-cas
#4
J C Green, J S Hu
This minireview summarizes recent advancements using the clustered regularly interspaced palindromic repeats-associated nuclease systems (CRISPR-Cas) derived from prokaryotes to breed plants resistant to DNA and RNA viruses. The CRISPR-Cas system represents a powerful tool able to edit and insert novel traits into plants precisely at chosen loci offering enormous advantages to classical breeding. Approaches to engineering plant virus resistance in both transgenic and non-transgenic plants are discussed. Iterations of the CRISPR-Cas system, FnCas9 and C2c2 capable of editing RNA in eukaryotic cells offer a particular advantage for providing resistance to RNA viruses which represent the great majority of known plant viruses...
2017: Acta Virologica
https://www.readbyqxmd.com/read/28522008/chitosan-chondroitin-sulfate-hydrogels-prepared-in-hmim-hso4-ionic-liquid
#5
Cátia S Nunes, Kessily B Rufato, Paulo R Souza, Elizângela A M S de Almeida, Michael J V da Silva, Débora B Scariot, Celso V Nakamura, Fernanda A Rosa, Alessandro F Martins, Edvani C Muniz
[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS). The use of IL to solubilize both polysaccharides was considered an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems. CHT/CS hydrogels were carried out in homogeneous medium from short dissolution times. The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28520756/de-novo-assembly-and-comparative-transcriptome-analysis-of-the-foot-from-chinese-green-mussel-perna-viridis-in-response-to-cadmium-stimulation
#6
Xinhui Zhang, Zhiqiang Ruan, Xinxin You, Jintu Wang, Jieming Chen, Chao Peng, Qiong Shi
The Chinese green mussel, Perna viridis, is a marine bivalve with important economic values as well as biomonitoring roles for aquatic pollution. Byssus, secreted by the foot gland, has been proved to bind heavy metals effectively. In this study, using the RNA sequencing technology, we performed comparative transcriptomic analysis on the mussel feet with or without inducing by cadmium (Cd). Our current work is aiming at providing insights into the molecular mechanisms of byssus binding to heavy metal ions. The transcriptome sequencing generated a total of 26...
2017: PloS One
https://www.readbyqxmd.com/read/28516669/state-of-the-art-novel-applications-for-deep-brain-stimulation
#7
REVIEW
Holly A Roy, Alexander L Green, Tipu Z Aziz
OBJECTIVES: Deep brain stimulation (DBS) is a rapidly developing field of neurosurgery with potential therapeutic applications that are relevant to conditions traditionally viewed as beyond the limits of neurosurgery. Our objective, in this review, is to highlight some of the emerging applications of DBS within three distinct but overlapping spheres, namely trauma, neuropsychiatry, and autonomic physiology. REVIEW METHODS: An extensive literature review was carried out in MEDLINE, to identify relevant studies and review articles describing applications of DBS in the areas of trauma, neuropsychiatry and autonomic neuroscience...
May 17, 2017: Neuromodulation: Journal of the International Neuromodulation Society
https://www.readbyqxmd.com/read/28516207/enhancing-h2o2-resistance-of-an-esterase-from-pyrobaculum-calidifontis-by-structure-guided-engineering-of-the-substrate-binding-site
#8
Pengfei Zhou, Dongming Lan, Grzegorz Maria Popowicz, Xuping Wang, Bo Yang, Yonghua Wang
Green technologies are attracting increasing attention in industrial chemistry where enzymatic reactions can replace dangerous and environmentally unfriendly chemical processes. In situ enzymatic synthesis of peroxycarboxylic acid is an attractive alternative for several industrial applications although concentrated H2O2 can denature the biocatalyst, limiting its usefulness. Herein, we report the structure-guided engineering of the Pyrobaculum calidifontis esterase (PestE) substrate binding site to increase its stability and perhydrolysis activity...
May 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28515385/supercritical-co2-extraction-of-rice-bran-oil-the-technology-manufacture-and-applications
#9
Phumon Sookwong, Sugunya Mahatheeranont
Rice bran is a good source of nutrients that have large amounts of phytochemicals and antioxidants. Conventional rice bran oil production requires many processes that may deteriorate and degrade these valuable substances. Supercritical CO2 extraction is a green alternative method for producing rice bran oil. This work reviews production of rice bran oil by supercritical carbon dioxide (SC-CO2) extraction. In addition, the usefulness and advantages of SC-CO2 extracted rice bran oil for edible oil and health purpose is also described...
May 17, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28514850/dna-dendrimer-streptavidin-nanocomplex-an-efficient-signal-amplifier-for-construction-of-biosensing-platforms
#10
Yan Zhao, Shichao Hu, Huaming Wang, Kaiwen Yu, Yan Guan, Xiaoyun Liu, Na Li, Feng Liu
We develop a DNA dendrimer-streptavidin (SA) nanocomplex as a novel signal amplifier to create biosensing platforms for disease-related species. The DNA dendrimer-SA nanocomplex is fabricated by crosslinking the nonlinear hybridization chain reaction based DNA dendrimer with the SA-coupled linker DNA, and possesses multiple sticky ends, a high molecular weight, and a hyperbranched nanostructure with large numbers of DNA duplexes. Taking advantages of the DNA dendrimer-SA nanocomplex and a label-free quartz crystal microbalance (QCM) technology, we first construct a mass-sensitive QCM biosensing platform for nucleic acids, which displays high selectivity and sensitivity with the detection limit of 0...
May 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28514025/acute-joint-pain-in-the-emerging-green-collar-workforce-evidence-from-the-linked-national-health-interview-survey-and-occupational-information-network-o-net
#11
Samuel R Huntley, David J Lee, William G LeBlanc, Kristopher L Arheart, Laura A McClure, Lora E Fleming, Alberto J Caban-Martinez
BACKGROUND: Green jobs are a rapidly emerging category of very heterogeneous occupations that typically involve engagement with new technologies and changing job demands predisposing them to physical stressors that may contribute to the development of joint pain. METHODS: We estimated and compared the prevalence of self-reported acute (past 30 days) joint pain between green and non-green collar workers using pooled 2004-2012 National Health Interview Survey (NHIS) data linked to the Occupational Information Network Database (O*NET)...
June 2017: American Journal of Industrial Medicine
https://www.readbyqxmd.com/read/28511723/feasibility-and-diagnostic-performance-of-dual-tracer-guided-sentinel-lymph-node-biopsy-in-ct1-2n0m0-gastric-cancer-a-systematic-review-and-meta-analysis-of-diagnostic-studies
#12
REVIEW
Ling Huang, Tao Wei, Junjun Chen, Donghui Zhou
BACKGROUND: Dual-tracer-guided sentinel lymph node (SLN) biopsy may provide a promising diagnostic tool to assess accurately the status of lymph node metastasis in the surgical operation and assure the oncologic safety of the function or stomach preserving surgery. The diagnostic performance of this technology in recent studies varied. Thus, we conducted this meta-analysis. METHODS: This systematic review and meta-analysis was registered at the PROSPERO. Eligible studies were searched in the PubMed, EMBASE, Web of Knowledge, and Cochrane Library databases...
May 16, 2017: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28508060/in-vivo-mapping-of-tissue-and-subcellular-specific-proteomes-in-caenorhabditis-elegans
#13
Aaron W Reinke, Raymond Mak, Emily R Troemel, Eric J Bennett
Multicellular organisms are composed of tissues that have distinct functions requiring specialized proteomes. To define the proteome of a live animal with tissue and subcellular resolution, we adapted a localized proteomics technology for use in the multicellular model organism Caenorhabditis elegans. This approach couples tissue- and location-specific expression of the enzyme ascorbate peroxidase (APX), which enables proximity-based protein labeling in vivo, and quantitative proteomics to identify tissue- and subcellular-restricted proteomes...
May 2017: Science Advances
https://www.readbyqxmd.com/read/28506879/a-new-platform-for-long-term-tracking-and-recording-of-neural-activity-and-simultaneous-optogenetic-control-in-freely-behaving-caenorhabditis-elegans
#14
Keiko Gengyo-Ando, Yuko Kagawa-Nagamura, Masamichi Ohkura, Xianfeng Fei, Min Chen, Koichi Hashimoto, Junichi Nakai
BACKGROUND: Real-time recording and manipulation of neural activity in freely behaving animals can greatly advance our understanding of how neural circuits regulate behavior. Ca(2+) imaging and optogenetic manipulation with optical probes are key technologies for this purpose. However, integrating the two optical approaches with behavioral analysis has been technically challenging. NEW METHOD: Here, we developed a new imaging system, ICaST (Integrated platform for Ca(2+) imaging, Stimulation, and Tracking), which combines an automatic worm tracking system and a fast-scanning laser confocal microscope, to image neurons of interest in freely behaving C...
May 12, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28504561/sexual-violence-and-intimate-partner-violence-in-college-women-with-a-mental-health-and-or-behavior-disability
#15
Amy Bonomi, Emily Nichols, Rebecca Kammes, Troye Green
OBJECTIVES: We address questions about (1) how college women with a disability experience sexual violence (SV) and intimate partner violence (IPV) across partners, including disability-specific abuse and (2) how SV/IPV impacts psychological, behavioral, physical, and academic life domains. METHODS/DESIGN: Twenty-seven female college students (mean age, 21.2; 66.6% white; 66.6% heterosexual) were randomly sampled from university registrar records. To be eligible for the study, students had to have at least one experience of SV/IPV since age 18 and a disability (88...
May 15, 2017: Journal of Women's Health
https://www.readbyqxmd.com/read/28501631/plasmid-transfection-in-bovine-cells-optimization-using-a-realtime-monitoring-of-green-fluorescent-protein-and-effect-on-gene-reporter-assay
#16
J S Osorio, M Bionaz
Gene reporter technology (GRT) has opened several new avenues for monitoring biological events including the activation of transcription factors, which are central to the study of nutrigenomics. However, this technology relies heavily on the insertion of foreign plasmid DNA into the nuclei of cells (i.e., transfection), which can be very challenging and highly variable among cell types. The objective of this study was to investigate the optimal conditions to generate reliable GRT assay data on bovine immortalized cell lines, Madin Darby Bovine Kidney (MDBK) and bovine mammary epithelial alveolar (MACT) cells...
May 10, 2017: Gene
https://www.readbyqxmd.com/read/28499771/the-key-role-of-the-meat-industry-in-transformation-to-a-low-carbon-climate-resilient-sustainable-economy
#17
Frank Rijsberman
Climate change, air pollution and refugees have become key global challenges threatening sustainability of lifestyles, economies and ecosystems. Agri-food systems are the number one driver of environmental change. Livestock production is the world's largest land user, responsible for half of greenhouse gas emissions from agri-food systems, and the source of repeated health crises. Poor diets have become the number one cause of ill health. Recommendations for a healthy diet emphasize plant-based food. Rapidly falling costs in information technology, biotechnology, renewable energy and battery technology will disrupt current energy and transportation systems and offer opportunities for responsible meat production...
April 26, 2017: Meat Science
https://www.readbyqxmd.com/read/28499433/servar-an-augmented-reality-tool-to-guide-the-serving-of-food
#18
Megan E Rollo, Tamara Bucher, Shamus P Smith, Clare E Collins
BACKGROUND: Accurate estimation of food portion size is a difficult task. Visual cues are important mediators of portion size and therefore technology-based aids may assist consumers when serving and estimating food portions. The current study evaluated the usability and impact on estimation error of standard food servings of a novel augmented reality food serving aid, ServAR. METHODS: Participants were randomised into one of three groups: 1) no information/aid (control); 2) verbal information on standard serving sizes; or 3) ServAR, an aid which overlayed virtual food servings over a plate using a tablet computer...
May 12, 2017: International Journal of Behavioral Nutrition and Physical Activity
https://www.readbyqxmd.com/read/28497776/using-shape-to-turn-off-blinking-for-two-colour-multiexciton-emission-in-cdse-cds-tetrapods
#19
Nimai Mishra, Noah J Orfield, Feng Wang, Zhongjian Hu, Sachidananda Krishnamurthy, Anton V Malko, Joanna L Casson, Han Htoon, Milan Sykora, Jennifer A Hollingsworth
Semiconductor nanostructures capable of emitting from two excited states and thereby of producing two photoluminescence colours are of fundamental and potential technological significance. In this limited class of nanocrystals, CdSe/CdS core/arm tetrapods exhibit the unusual trait of two-colour (red and green) multiexcitonic emission, with green emission from the CdS arms emerging only at high excitation fluences. Here we show that by synthetic shape-tuning, both this multi-colour emission process, and blinking and photobleaching behaviours of single tetrapods can be controlled...
May 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/28490100/plasma-modification-of-starch
#20
REVIEW
Fan Zhu
Plasma is a medium of unbound negative and positive particles with the overall electrical charge being roughly zero. Non-thermal plasma processing is an emerging green technology with great potential to improve the quality and microbial safety of various food materials. Starch is a major component of many food products and is an important ingredient for food and other industries. There has been increasing interest in utilizing plasma to modify the functionalities of starch through interactions with reactive species...
October 1, 2017: Food Chemistry
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