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https://www.readbyqxmd.com/read/27916373/antibiotic-releasing-microspheres-prevent-mesh-infection-in%C3%A2-vivo
#1
Kevin T Grafmiller, Sean T Zuckerman, Clayton Petro, Lijia Liu, Horst A von Recum, Michael J Rosen, Julius N Korley
BACKGROUND: Infection remains a dreaded complication after implantation of surgical prosthetics, particularly after hernia repair with synthetic mesh. We previously demonstrated the ability of a newly developed polymer to provide controlled release of an antibiotic in a linear fashion over 45 d. We subsequently showed that coating mesh with the drug-releasing polymer prevented a Staphylococcus aureus (SA) infection in vivo. To broaden the applicability of this technology, the polymer was synthesized as isolated "microspheres" and loaded with vancomycin (VM) before conducting a noninferiority analysis...
November 2016: Journal of Surgical Research
https://www.readbyqxmd.com/read/27915473/urine-proteomics-in-the-era-of-mass-spectrometry
#2
REVIEW
Ashley Beasley-Green
With the technological advances of mass spectrometry (MS)-based platforms, clinical proteomics is one of the most rapidly growing areas in biomedical research. Urine proteomics has become a popular subdiscipline of clinical proteomics because it is an ideal source for the discovery of noninvasive disease biomarkers. The urine proteome offers a comprehensive view of the local and systemic physiology since the proteome is primarily composed of proteins/peptides from the kidneys and plasma. The emergence of MS-based proteomic platforms as prominent bioanalytical tools in clinical applications has enhanced the identification of protein-based urinary biomarkers...
November 2016: International Neurourology Journal
https://www.readbyqxmd.com/read/27915037/quantification-of-pea-enation-mosaic-virus-1-and-2-during-infection-of-pisum-sativum-by-one-step-real-time-rt-pcr
#3
Juliette Doumayrou, Melissa Sheber, Bryony C Bonning, W Allen Miller
Pea enation mosaic virus 1 (PEMV1) and Pea enation mosaic virus 2 (PEMV2) are two viruses in an obligate symbiosis that cause pea enation mosaic disease mainly in plants in the Fabaceae family. This virus system is a valuable model to investigate plant virus replication, movement and vector transmission. Thus, here we describe growth conditions, virus detection methods, and virus accumulation behavior. To measure the accumulation and movement of PEMV1 and PEMV2 in plants during the course of infection, we developed a quantitative real-time one-step reverse transcription PCR procedure using the SYBR-green(®) technology...
November 30, 2016: Journal of Virological Methods
https://www.readbyqxmd.com/read/27909212/black-carbon-emission-reduction-strategies-in-healthcare-industry-for-effective-global-climate-change-management
#4
Emilia Mmbando Raila, David O Anderson
Climate change remains one of the biggest threats to life on earth to date with black carbon (BC) emissions or smoke being the strongest cause after carbon dioxide (CO2). Surprisingly, scientific evidence about black carbon emissions reduction in healthcare settings is sparse. This paper presents new research findings on the reduction of black carbon emissions from an observational study conducted at the UN Peacekeeping Operations (MINUSTAH) in Haiti in 2014. Researchers observed 20 incineration cycles, 30 minutes for each cycle of plastic and cardboard sharps healthcare waste (HCW) containers ranged from 3 to 14...
November 30, 2016: Waste Management & Research
https://www.readbyqxmd.com/read/27908566/update-of-choroidal-imaging-techniques-past-present-and-future
#5
J Ruiz-Medrano, I Flores-Moreno, R Gutierrez-Bonet, J Chhablani, J M Ruiz-Moreno
The choroid is the middle layer of the eye, a very vascular and pigmented tissue, with its role in several ophthalmological pathologies already having been clearly established. But it was not until the last few years that we have been able to reliably and precisely measure and quantify its shape and thickness. Ultrasound technology and indocyanine green angiography were the first techniques used for the study of the choroid, and they still maintain their use and clinical indications for the diagnosis and management of several pathologies...
November 28, 2016: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/27901630/emerging-pollutant-treatments-in-wastewater-cases-of-antibiotics-and-hormones
#6
Erika Méndez, Miguel A González-Fuentes, Georgette Rebollar-Perez, Alia Méndez-Albores, Eduardo Torres
Because of the intensive use of pharmaceutical substances in human life, studies on the detection of these chemical compounds and their metabolites as pollutants in water bodies are continuously reported. Some pharmaceutical agents are associated with adverse effects to aquatic life, even at very low concentrations (ng L(-1) to μg L(-1)). For instance, the presence of antibiotics and hormones has been associated with increasing proliferation of antibiotic resistant pathogens and feminization and masculinization of some aquatic organisms...
November 30, 2016: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/27900475/microclimatic-effects-of-planted-hydroponic-structures-in-urban-environment-measurements-and-simulations
#7
N Katsoulas, D Antoniadis, I L Tsirogiannis, E Labraki, T Bartzanas, C Kittas
The objectives of this effort was to study the effect of vertical (green wall) and horizontal (pergola) green structures on the microclimate conditions of the building surroundings and estimate the thermal perception and heat stress conditions near the two structures. The experimental data were used to validate the results simulated by the recent version (V4.0 preview III) of ENVI-met software which was used to simulate the effect of different design parameters of a pergola and a green façade on microclimate and heat stress conditions...
November 29, 2016: International Journal of Biometeorology
https://www.readbyqxmd.com/read/27898471/green-disease-in-optical-coherence-tomography-diagnosis-of-glaucoma
#8
Mohamed S Sayed, Michael Margolis, Richard K Lee
PURPOSE OF REVIEW: Optical coherence tomography (OCT) has become an integral component of modern glaucoma practice. Utilizing color codes, OCT analysis has rendered glaucoma diagnosis and follow-up simpler and faster for the busy clinician. However, green labeling of OCT parameters suggesting normal values may confer a false sense of security, potentially leading to missed diagnoses of glaucoma and/or glaucoma progression. RECENT FINDINGS: Conditions in which OCT color coding may be falsely negative (i...
November 24, 2016: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/27895581/evaluation-of-near-infrared-dyes-as-markers-of-p-glycoprotein-activity-in-tumors
#9
Inessa Semenenko, Emma Portnoy, Mohammed Aboukaoud, Serge Guzy, Miriam Shmuel, Gal Itzhak, Sara Eyal
Aim: The multidrug resistance protein 1 (MDR1; P-glycoprotein) has been associated with efflux of chemotherapeutic agents from tumor cells and with poor patient prognosis. This study evaluated the feasibility of non-invasive, non-radioactive near infrared (NIR) imaging methodology for detection of MDR1 functional activity in tumors. Methods: Initial accumulation assays were conducted in MDR1-overexpressing MDCK cells (MDCK-MDR1) and control MDCK cells (MDCK-CT) using the NIR dyes indocyanine green (ICG), IR-783, IR-775, rhodamine 800, XenoLight DiR, and Genhance 750, at 0...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27894697/an-innovative-ultrasound-assisted-extraction-micro-scale-cell-combined-with-gas-chromatography-mass-spectrometry-in-negative-chemical-ionization-to-determine-persistent-organic-pollutants-in-air-particulate-matter
#10
E Beristain-Montiel, R Villalobos-Pietrini, G E Arias-Loaiza, S L Gómez-Arroyo, O Amador-Muñoz
New clean technologies are needed to determine concentration of organic pollutants without generating more pollution. A method to extract Persistent Organic Pollutants (POPs) from airborne particulate matter was developed using a novel technology recently patented called ultrasound assisted extraction micro-scale cell (UAE-MSC). This technology extracts, filters, collects the sample, and evaporates the solvent, on-line. No sample transfer is needed. The cell minimizes sample manipulation, solvent consumption, waste generation, time, and energy; fulfilling most of the analytical green chemistry protocol...
November 23, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27889241/natural-and-synthetic-coral-biomineralization-for-human-bone-revitalization
#11
REVIEW
David W Green, Besim Ben-Nissan, Kyung Sik Yoon, Bruce Milthorpe, Han-Sung Jung
Coral skeletons can regenerate replacement human bone in nonload-bearing excavated skeletal locations. A combination of multiscale, interconnected pores and channels and highly bioactive surface chemistry has established corals as an important alternative to using healthy host bone replacements. Here, we highlight how coral skeletal systems are being remolded into new calcified structures or synthetic corals by biomimetic processes, as places for the organized permeation of bone tissue cells and blood vessels...
November 23, 2016: Trends in Biotechnology
https://www.readbyqxmd.com/read/27881977/different-virus-derived-sirnas-profiles-between-leaves-and-fruits-in-cucumber-green-mottle-mosaic-virus-infected-lagenaria-siceraria-plants
#12
Junmin Li, Hongying Zheng, Chenhua Zhang, Kelei Han, Shu Wang, Jiejun Peng, Yuwen Lu, Jinping Zhao, Pei Xu, Xiaohua Wu, Guojing Li, Jianping Chen, Fei Yan
RNA silencing is an evolutionarily conserved antiviral mechanism, through which virus-derived small interfering RNAs (vsiRNAs) playing roles in host antiviral defense are produced in virus-infected plant. Deep sequencing technology has revolutionized the study on the interaction between virus and plant host through the analysis of vsiRNAs profile. However, comparison of vsiRNA profiles in different tissues from a same host plant has been rarely reported. In this study, the profiles of vsiRNAs from leaves and fruits of Lagenaria siceraria plants infected with Cucumber green mottle mosaic virus (CGMMV) were comprehensively characterized and compared...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27879622/ultrasound-assisted-extraction-centrifugation-and-ultrafiltration-multistage-process-for-polyphenol-recovery-from-purple-sweet-potatoes
#13
Zhenzhou Zhu, Tian Jiang, Jingren He, Francisco J Barba, Giancarlo Cravotto, Mohamed Koubaa
This work provides an evaluation of an ultrasound-assisted, combined extraction, centrifugation and ultrafiltration process for the optimal recovery of polyphenols. A purple sweet potato (PSP) extract has been obtained using ultrasonic circulating extraction equipment at a power of 840 W, a frequency of 59 kHz and using water as solvent. Extract ultrafiltration, using polyethersulfone (PES), was carried out for the recovery of polyphenol, protein and anthocyanin. Pre-treatment, via the centrifugation of purple sweet potato extract at 2500 rpm over 6 min, led to better polyphenol recovery, with satisfactory protein removal (reused for future purposes), than PSP extract filtration without centrifugation...
November 20, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27879398/spectral-unmixing-plate-reader-high-throughput-high-precision-fret-assays-in-living-cells
#14
Tory M Schaaf, Kurt C Peterson, Benjamin D Grant, David D Thomas, Gregory D Gillispie
We have developed a microplate reader that records a complete high-quality fluorescence emission spectrum on a well-by-well basis under true high-throughput screening (HTS) conditions. The read time for an entire 384-well plate is less than 3 min. This instrument is particularly well suited for assays based on fluorescence resonance energy transfer (FRET). Intramolecular protein biosensors with genetically encoded green fluorescent protein (GFP) donor and red fluorescent protein (RFP) acceptor tags at positions sensitive to structural changes were stably expressed and studied in living HEK cells...
November 22, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27872814/transcriptional-profiles-of-wnv-neurovirulence-in-a-genetically-diverse-collaborative-cross-population
#15
Richard Green, Courtney Wilkins, Sunil Thomas, Aimee Sekine, Renee C Ireton, Martin T Ferris, Duncan M Hendrick, Kathleen Voss, Fernando Pardo-Manuel de Villena, Ralph S Baric, Mark T Heise, Michael Gale
West Nile Virus (WNV) is a mosquito-transmitted virus from the Flaviviridae family that causes fever in 1 in 5 infected people. WNV can also become neuro-invasive and cross the blood-brain barrier leading to severe neurological symptoms in a subset of WNV infected individuals [1]. WNV neuro-invasion is believed to be influenced by a number of factors including host genetics. In order to explore these effects and recapitulate the complex immune genetic differences among individuals, we studied gene expression following WNV infection in the Collaborative Cross (CC) model...
December 2016: Genomics Data
https://www.readbyqxmd.com/read/27869267/the-reaction-mechanism-of-polyalcohol-dehydration-in-hot-pressurized-water
#16
Sergi Ruiz-Barragan, Jordi Ribas Ariño, Motoyuki Shiga
The use of high-temperature liquid water (HTW) as a reaction medium is a very promising technology in the field of green chemistry. In order to fully exploit this technology, it is crucial to unravel the reaction mechanisms of the processes carried out in HTW. In this work, the reaction mechanism of 2,5-hexanediol dehydration in HTW has been studied by means of three different ab initio simulations: the string method, metadynamics and molecular dynamics in real time. It is found that the whole reaction involving protonation, bond exchange and deprotonation occurs in a single step without a stable intermediate...
November 30, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27866608/noble-metal-oxide-and-chalcogenide-based-nanomaterials-from-scalable-phototrophic-culture-systems
#17
Si Amar Dahoumane, Evan K Wujcik, Clayton Jeffryes
Phototrophic cell or tissue cultures can produce nanostructured noble metals, oxides and chalcogenides at ambient temperatures and pressures in an aqueous environment and without the need for potentially toxic solvents or the generation of dangerous waste products. These "green" synthesized nanobiomaterials can be used to fabricate biosensors and bio-reporting tools, theranostic vehicles, medical imaging agents, as well as tissue engineering scaffolds and biomaterials. While successful at the lab and experimental scales, significant barriers still inhibit the development of higher capacity processes...
December 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27866007/continuous-manufacturing-via-hot-melt-extrusion-and-scale-up-regulatory-matters
#18
REVIEW
Mohammed Maniruzzaman, Ali Nokhodchi
Currently, because globalization, the pharmaceutical industry is facing enormous challenges to comply with regulatory matters. Reduced patent life and overall decreased profitability of newly discovered drugs are also forcing the pharmaceutical industry to shorten the drug development time with maximum throughput. Therefore, continuous manufacturing (CM) processes via hot melt extrusion (HME) can be a promising alternative for achieving these goals. HME offers solvent-free green technology with a process that is easy to scale up...
November 16, 2016: Drug Discovery Today
https://www.readbyqxmd.com/read/27864717/intraoperative-localization-of-the-parathyroid-glands-with-indocyanine-green-and-firefly-r-technology-during-baba-robotic-thyroidectomy
#19
Hyeong Won Yu, Joon Woo Chung, Jin Wook Yi, Ra-Yeong Song, Joon-Hyop Lee, Hyungju Kwon, Su-Jin Kim, Young Jun Chai, June Young Choi, Kyu Eun Lee
BACKGROUND: It is unclear whether near-infrared (NIR) light-induced indocyanine green (ICG) fluorescence can effectively identify, and thus permit the preservation of, parathyroid glands in bilateral axillo-breast approach (BABA) robotic thyroidectomy. This case-control study with a prospectively recruited consecutive series and a retrospectively selected control group assessed the usefulness of ICG with Firefly(R) technology to identify the parathyroid glands intraoperatively during BABA robotic thyroidectomy...
November 18, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27863797/stabilization-of-pb-ii-accumulated-in-biomass-through-phosphate-pretreated-pyrolysis-at-low-temperatures
#20
Saijun Li, Tao Zhang, Jianfa Li, Lingna Shi, Xiaoxiao Zhu, Jinhong Lü, Yimin Li
The remediation of heavy metal-contaminated soil and water using plant biomass is considered to be a green technological approach, although the harmless disposal of biomass accumulated with heavy metals remains a challenge. A potential solution to this problem explored in this work involves combining phosphate pretreatment with pyrolysis. Pb(II) was accumulated in celery biomass with superior sorption capacity and also in ordinary wood biomass through biosorption. The Pb(II)-impregnated biomass was then pretreated with phosphoric acid or calcium dihydrogen phosphate (CaP) and pyrolyzed at 350 or 450°C...
November 10, 2016: Journal of Hazardous Materials
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