keyword
MENU ▼
Read by QxMD icon Read
search

Rsd

keyword
https://www.readbyqxmd.com/read/28445409/quantification-of-a-novel-photosensitizer-of-chlorin-e6-c15-monomethyl-ester-in-beagle-dog-plasma-using-hplc-application-to-pharmacokinetic-studies
#1
Xiuxiu Li, Jun Wen, Jingjing Jiang, Xin Zhao, Tingting Zhou, Guorong Fan
Chlorin e6-C15-monomethyl ester (CMME) is a novel photosensitizer, which is synthetized from the degradation products of silkworm excrement. Preclinical studies on the promising photosensitizer CMME are necessary to determine its therapeutic efficacy and druglikeness. A high-performance liquid chromatography with UV detection (HPLC-UV) method was established for the determination of CMME in beagle dog plasma. The sample preparation involved a protein-precipitation method with acetonitrile after the addition of tanshinone IIA as an internal standard (IS)...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28444375/evaluation-of-the-alpha-amylase-activity-as-an-indicator-of-pasteurization-efficiency-and-microbiological-quality-of-liquid-whole-eggs
#2
Guilherme Resende da Silva, Liliane Denize Miranda Menezes, Isabela Pereira Lanza, Daniela Duarte de Oliveira, Carla Aparecida Silva, Roger Wilker Tavares Klein, Débora Cristina Sampaio de Assis, Silvana de Vasconcelos Cançado
In order to evaluate the efficiency of the pasteurization process in liquid whole eggs, an UV/visible spectrophotometric method was developed and validated for the assessment of alpha-amylase activity. Samples were collected from 30 lots of raw eggs (n = 30) and divided into three groups: one was reserved for analysis of the raw eggs, the second group was pasteurized at 61.1°C for 3.5 minutes (n = 30), and the third group was pasteurized at 64.4°C for 2.5 minutes (n = 30). In addition to assessing alpha-amylase activity, the microbiological quality of the samples was also evaluated by counting total and thermotolerant coliforms, mesophilic aerobic microorganisms, Staphylococcus spp...
April 24, 2017: Poultry Science
https://www.readbyqxmd.com/read/28442131/multipoint-recognition-of-domoic-acid-from-seawater-by-dummy-template-molecularly-imprinted-solid-phase-extraction-coupled-with-high-performance-liquid-chromatography
#3
Xiuping He, Jianlei Chen, Jiangtao Wang, Liju Tan
Due to the high cost of domoic acid (DA), different carboxylic acid compounds including indole-3-acetic acid (IAA), pyrrole-2-formic acid (PFA), pyridine-2,3-dicarboxylic anhydride (PDA), trimesitinic acid (TA) and citric acid (CA) were investigated as dummy templates for the molecularly imprinted solid-phase extraction (MISPE) for selective isolation and pre-concentration of an amnesic shellfish poison (ASP), DA. The highest binding amount of the polymers towards DA was obtained when CA was used as dummy template owing to its high hydrophilicity...
April 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28441352/high-throughput-determination-of-sodium-danshensu-in-beagle-dogs-by-the-lcms-ms-method-employing-liquid-liquid-extraction-based-on-96-well-format-plates
#4
Jingjing Jiang, Xin Zhao, Xiuxiu Li, Shengyuan Wu, Shidan Yu, Yuefen Lou, Guorong Fan
Sodium Danshensu (sodium d-(+)-β-(3,4-dihydroxyphenyl) lactate), one of the water-soluble ingredients in Salvia miltiorrhiza, exhibits potent relaxation of the coronary artery and anticoagulation effection. A high-throughput, rapid, and sensitive method combining liquid chromatography with electrospray ionization tandem mass spectrometry to determine the sodium danshensu in beagle dog plasma was developed and validated, using gallic acid as an internal standard (IS). Acidified plasma samples were extracted using 96-well liquid-liquid extraction, and were eluted on a CNW Athena C18 column (3 μm, 2...
April 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28437686/synthesis-optical-properties-and-application-of-a-set-of-novel-pyrazole-nopinone-derivatives
#5
Jinlai Yang, Xu Xu, Jian Rui, Zhonglong Wang, Yan Zhang, Shifa Wang, Liangru Wu
Pyrazole derivatives (4-6) were directly synthesized from β-pinene derivative nopinone, and they were characterized by Fourier transform infrared (FTIR) spectoscope, nuclear magnetic resonance (NMR), and mass spectrometry. Their optical properties were investigated by ultraviolet-visible spectroscopy and fluorescence spectroscopy. The three compounds emitted strong blue fluorescence in ethanol. Using a fluorescence quenching method, compound 4 could be used to detect the content (100.57%) of copper sulfate pentahydrate (≥99%) with a RSD of 1...
April 20, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28437060/high-throughput-and-quantitative-measurement-of-microbial-metabolome-by-gas-chromatography-mass-spectrometry-using-automated-alkyl-chloroformate-derivatization
#6
Linjing Zhao, Yan Ni, Mingming Su, Hongsen Li, Fangcong Dong, Wenlian Chen, Runmin Wei, Lulu Zhang, Seu-Ping Guiraud, François-Pierre J Martin, Cynthia Rajani, Guoxiang Xie, Wei Jia
The ability to identify and quantify small molecule metabolites derived from gut microbial-mammalian co-metabolism is essential for the understanding of the distinct metabolic functions of the microbiome. To date, analytical protocols that quantitatively measure a complete panel of microbial metabolites in biological samples have not been established, but urgently needed by the microbiome research community. Here, we report an automated high-throughput quantitative method using a gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) platform to simultaneously measure over one hundred microbial metabolites in human serum, urine, feces and Escherichia coli cell samples within 15 minutes per sample...
April 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28433060/enhanced-photoresponsive-polyethyleneimine-citric-acid-co-carbonized-dots-for-facile-and-selective-sensing-and-intracellular-imaging-of-cobalt-ions-at-physiologic-ph
#7
Wen-Sheng Zou, Qing-Chun Zhao, Jun Zhang, Xiao-Ming Chen, Xiu-Fang Wang, Lin Zhao, Shao-Hua Chen, Ya-Qin Wang
Whether as an important biological element or as a radioactive source/medicine, the monitoring of trace levels of cobalt ions (Co) has become a non-negligible factor for human health and green environment. Current technologies for the detection of Co are cost-expensive and time-consuming, and require cumbersome sample pretreatment process. Herein a novel sensing platform has been developed for Co detection based on the quenching of the enhanced fluorescence signal of polyamine functionalized C-dots. Amine groups at the surface of the C-dots can capture Zn(2+)/Cd(2+) to form coordination compound, which can inhibit the photoinduced electron transfer pathways of C-dots and then induce the fluorescence enhancement of the C-dots by ∼80% margin...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28432941/validation-of-a-spe-hplc-uv-method-for-the-quantification-of-a-new-er-specific-photosensitizer-or-141-in-blood-serum-using-total-error-concept
#8
Emilie Bony, Yohan Mace, Adán Pinto, David Delvaux, Robert Kiss, Olivier Riant, Olivier Feron, Joëlle Quetin-Leclercq
The aim of this work is to develop the first validated HPLC-UV method quantification in blood serum for a new endoplasmic reticulum (ER)-specific benzophenazine photosensitizer (OR-141). A fast solid phase extraction (SPE) cleaning sample procedure was achieved on C18 encapped (ec) SPE cartridges and the separation was performed on a RP-18e column (5μM) using an isocratic elution with methanol. The method has been fully validated according to accuracy profiles based on total error and tolerance intervals. Calibration was performed in the matrix and trueness (<4...
April 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28431149/next-generation-non-starch-polysaccharide-degrading-multi-carbohydrase-complex-rich-in-xylanase-and-arabinofuranosidase-to-enhance-broiler-feed-digestibility
#9
Pierre Cozannet, Michael T Kidd, Roberto Montanhini Neto, Pierre-André Geraert
This study was carried out to evaluate the effect of a multi-carbohydrase complex ( ) rich in xylanase ( ) and arabinofuranosidase ( ) on overall broiler feed digestibility in broilers. Energy utilization and digestibility of dry matter ( ), organic matter ( ), protein, starch, fat, and insoluble and soluble fibers were measured using the mass-balance method. The experiment was carried out on 120 broilers (3-week-old chickens). Broilers were distributed over 8 treatments to evaluate the effect of the dietary arabinoxylan content and nutrient density with and without MCC (Rovabio® Advance)...
April 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/28429676/determination-of-dinotefuran-and-its-metabolites-in-orange-pulp-orange-peel-and-whole-orange-using-liquid-chromatography-tandem-mass-spectrometry
#10
Zaihui Yang, Kankan Zhang, Lingzhu Chen, Bin Liu, Qingtao Zhang, Haizhen Zhang, Caiyuan Sun, Deyu Hu
Determination of the polar characteristics of dinotefuran and metabolite residues in orange matrixes (orange pulp, orange peel, and whole orange) is difficult. Thus, the purpose of the present study was to develop an extraction method for the determination of dinotefuran and its metabolites in oranges by using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Matrix suppression effects were observed for all analytes in the orange matrixes. The proposed method displayed satisfactory linearity (R(2) ≥ 0...
April 21, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28427003/ce-method-for-the-in-process-control-of-the-synthesis-of-active-substances-conjugated-with-gold-nanoparticles
#11
Wioleta Maruszak, Elżbieta U Stolarczyk, Krzysztof Stolarczyk
A fast capillary electrophoresis method was developed and validated for the in-process control (IPC) of the synthesis of active substances (APIs) with gold nanoparticles (AuNPs). The capillary electrophoresis method was key to ensure that the reaction step conducted in order to obtain AuNP and API conjugates will produce the expected product without the presence of free APIs, which is a critical parameter determining the quality of the synthetic material. Capillary electrophoresis was performed using uncoated fused-silica capillaries with the effective length of 40cm, 50μm i...
April 10, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28426177/an-efficient-cleanup-method-coupled-with-gas-chromatography-and-mass-spectrometry-for-multi-pesticides-residue-analysis-in-complex-plant-matrices
#12
Xinquan Wang, Peipei Qi, Xiangyun Wang, Qian Zhang, Zhiwei Wang, Xiahong Xu, Hao Xu, Hu Zhang, Qiang Wang
We aimed to develop an efficient cleanup method for multi-pesticides residue analysis in complex plant matrices, shallot, ginger, garlic, onion, leek and celery. Column chromatography was used as the cleanup method and fabricated with florisil and graphitized carbon black as the adsorbents. The amount of the graphitized carbon black adsorbent and the choice of the elution solvent were systematically investigated for exploring the best combination. The target pesticides covered organochlorine, pyrethroid and organophosphorus pesticides, and were 38 in total...
April 20, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28421988/composite-magnetic-nanoparticles-cufe2o4-as-a-new-microsorbent-for-extraction-of-rhodamine-b-from-water-samples
#13
Ali Roostaie, Farzad Allahnoori, Shokooh Ehteshami
In this work, novel composite magnetic nanoparticles (CuFe2O4) were synthesized based on sol-gel combustion in the laboratory. Next, a simple production method was optimized for the preparation of the copper nanoferrites (CuFe2O4), which are stable in water, magnetically active, and have a high specific area used as sorbent material for organic dye extraction in water solution. CuFe2O4 nanopowders were characterized by field-emission scanning electron microscopy (SEM), FTIR spectroscopy, and energy dispersive X-ray spectroscopy...
April 18, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28421986/all-solid-state-pvc-membrane-and-carbon-paste-ion-selective-electrodes-for-determination-of-donepezil-hydrochloride-in-pharmaceutical-formulation
#14
Nesreen Khamees, Tagreed Abdel-Fattah Mohamed, Abeer Rashad Derar, Azza Aziz
All-solid-state, polyvinyl chloride (PVC) membrane, and carbon paste potentiometric ion-selective electrodes (ISEs) were proposed for the determination of donepezil hydrochloride (DON) in the drug substance and a pharmaceutical formulation. The potentiometric response toward DON was based on the existence of donepezil-tetraphenyl borate (DON-TPB) in a PVC membrane or a carbon paste in the presence of dioctylphthalate. In contrast, the solid-state electrode was prepared by direct incorporation of DON-TPB into a commercial nail varnish without external additives...
April 18, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28420528/two-dimensional-gas-chromatography-with-electron-capture-detection-for-the-analysis-of-atmospheric-ozone-depleting-halocarbons
#15
Chang-Feng Ou-Yang, Hsi-Che Hua, Yu-Chieh Chou, Ming-Kai Teng, Wen-Tzu Liu, Jia-Lin Wang
This study is to develop a GC×GC method with electron capture detection (ECD) to analyze atmospheric halocarbons in the concentration range of parts per trillion by volume (pptv). To enrich atmospheric halocarbons a home-built thermal desorption (TD) device was coupled to the GC×GC-ECD. The technique of flow modulation was adopted using a Deans switch for GC×GC. Several column combinations of first and second dimensions were tested and the column set of DB-5×TG-1301 was found to show the best orthogonality for halocarbons...
April 4, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28419125/mammographic-compression-in-asian-women
#16
Susie Lau, Yang Faridah Abdul Aziz, Kwan Hoong Ng
OBJECTIVES: To investigate: (1) the variability of mammographic compression parameters amongst Asian women; and (2) the effects of reducing compression force on image quality and mean glandular dose (MGD) in Asian women based on phantom study. METHODS: We retrospectively collected 15818 raw digital mammograms from 3772 Asian women aged 35-80 years who underwent screening or diagnostic mammography between Jan 2012 and Dec 2014 at our center. The mammograms were processed using a volumetric breast density (VBD) measurement software (Volpara) to assess compression force, compression pressure, compressed breast thickness (CBT), breast volume, VBD and MGD against breast contact area...
2017: PloS One
https://www.readbyqxmd.com/read/28416644/evaluation-of-different-estimation-methods-for-accuracy-and-precision-in-biological-assay-validation
#17
Binbing Yu, Harry Yang
Biological assays (bioassays) are procedures to estimate the potency of a substance by studying its effects on living organisms, tissues, and cells. Bioassays are essential tools for gaining insight into biologic systems and processes including, for example, the development of new drugs and monitoring environmental pollutants. Two of the most important parameters of bioassay performance are relative accuracy (bias) and precision. Although general strategies and formulas are provided in USP 〈1033〉, a comprehensive understanding of the definitions of bias and precision remain elusive...
April 17, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28415795/renal-sympathetic-denervation-guided-by-renal-nerve-stimulation-to-treat-ventricular-arrhythmia-in-ckd-patients-with-icd
#18
Márcio Galindo Kiuchi, Shaojie Chen, Luis Marcelo Rodrigues Paz, Helmut Pürerfellner
Chronic kidney disease (CKD) patients on stage 4 present greater risk rates for malignant ventricular arrhythmia events. This study examined patients with CKD in stages 1, 2, 3 and 4, left ventricular dysfunction and automatic implantable cardioverter-defibrillator (ICD). Our goal was to record the appropriate therapies, "Anti-tachycardia Therapy Pacing" (ATP) and shock events during the 18 months of follow-up and compare the incidence and severity of these at different stages of CKD, mainly in patients with CKD stage 4 underwent renal sympathetic denervation (RSD) guided by renal nerve stimulation (RNS)...
March 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415020/simultaneous-determination-of-ten-bioactive-constituents-of-sanjie-zhentong-capsule-in-rat-plasma-by-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-and-its-application-to-a-pharmacokinetic-study
#19
Jiachun Li, Dongpo Li, Youzhi Pan, Jun-Hua Hu, Wenzhe Huang, Zhen-Zhong Wang, Wei Xiao, Yu Wang
Sanjie Zhentong capsule, a well-known traditional Chinese medicine prescription, are used for the treatment of endometriosis-related diseases. In this study, a simple, rapid and sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of ten bioactive constituents, including peimine, peiminine, peimisine, loureirin A, loureirin B, 7,4'-dihydroxyflavone, pterostilbene, ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 in rat plasma after oral administration of Sanjie Zhentong capsule...
March 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28414972/an-lc-ms-ms-method-for-quantification-of-ac0010-a-novel-mutant-selective-epidermal-growth-factor-receptor-egfr-inhibitor-and-its-metabolites-in-human-plasma-and-the-application-to-a-pharmacokinetic-study
#20
Lu Wang, Xin Zheng, Weicong Wang, Pei Hu, Ji Jiang
AC0010 is an irreversible, mutant-selective EGFR inhibitor that effectively inhibits EGFR active and T790M resistance mutations in non-small cell lung cancer (NSCLC). A sensitive ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) method was developed and fully validated for determining AC0010 and its metabolites in human plasma. The samples were purified by solid-phase extraction (SPE) columns and separated on a BEH C18 column. Electrospray ionization (ESI) in positive ion mode and multiple reaction monitoring (MRM) were used to monitor the ion transitions of AC0010 (m/z 488→257) and its metabolites M1 (m/z 474→403), M2 (m/z 504→487), M4 (m/z 434→377), M7 (m/z 490→405), MII-1 (m/z 651→434) and MII-2 (m/z 609→434)...
March 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
keyword
keyword
750
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"