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https://www.readbyqxmd.com/read/29928970/gallic-acid-coated-sliver-nanoparticle-alters-the-expression-of-radiation-induced-epithelial-mesenchymal-transition-in-non-small-lung-cancer-cells
#1
S N Sunil Gowda, S Rajasowmiya, V Vadivel, S Banu Devi, A Celestin Jerald, S Marimuthu, N Devipriya
BACKGROUND: Radiotherapy is the most widely used treatment method for treating cancer with or without surgery and chemotherapy. In lung cancer, it is one of the important treatment steps in excising the tumor from the lung tissue; unfortunately, radiation can induce epithelial- mesenchymal transition (EMT), a typical physiological process in which cuboidal shaped epithelial cell loses its phenotype and acquires mesenchymal-like phenotype thus, increases the metastasis progression in the body...
June 18, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29928866/hepatitis-b-virus-promotes-proliferation-and-metastasis-in-male-chinese-hepatocellular-carcinoma-patients-through-the-lef-1-mir-371a-5p-srcin1-pleiotrophin-slug-pathway
#2
Pei-Song Bai, Peng Hou, Ying Kong
Hepatocellular carcinoma (HCC) is a male-dominant cancer. Several factors may contribute to the gender difference. Recent investigations have reported that miRNAs are involved in sex-linked signaling pathways and play a critical role in the molecular pathogenesis of hepatitis B virus (HBV)-related HCC. Therefore, we speculated that some of these miRNAs might contribute to the gender differences observed in HBV-related HCC. Our results showed that miR-371a-5p was significantly upregulated in tumor tissue and serum from HCC patients and that the expression level of miR-371a-5p was related to HBV infection, sexuality, TNM stage, adjacent organ invasion and microvascular invasion...
June 18, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29928489/smad-proteins-directly-suppress-pax2-transcription-downstream-of-transforming-growth-factor-beta-1-tgf-%C3%AE-1-signalling-in-renal-cell-carcinoma
#3
Gagandeep Kaur, Caiyun Grace Li, Andrew Chantry, Cherie Stayner, Julia Horsfield, Michael R Eccles
Canonical TGF-β1 signalling promotes tumor progression by facilitating invasion and metastasis, whereby release of TGF-β1, by (for example) infiltrating immune cells, induces epithelial to mesenchymal transition (EMT). PAX2, a member of the Paired box family of transcriptional regulators, is normally expressed during embryonic development, including in the kidney, where it promotes mesenchymal to epithelial transition (MET). PAX2 expression is silenced in many normal adult tissues. However, in contrast, PAX2 is expressed in several cancer types, including kidney, prostate, breast, and ovarian cancer...
June 1, 2018: Oncotarget
https://www.readbyqxmd.com/read/29928474/cryptotanshinone-hinders-renal-fibrosis-and-epithelial-transdifferentiation-in-obstructive-nephropathy-by-inhibiting-tgf-%C3%AE-1-smad3-integrin-%C3%AE-1-signal
#4
Wei Wang, Pang-Hu Zhou, Wei Hu, Chang-Geng Xu, Xiang-Jun Zhou, Chao-Zhao Liang, Jie Zhang
Recent studies have reported that CTS can alleviate cardiac fibrosis. However, the effects of CTS on kidney fibrosis and EMT are still unknown. This study explored whether CTS could attenuate tubulointerstitial fibrosis as well as EMT, and investigated the potential underlying mechanisms. In this study, an in vivo UUO mouse model and an in vitro TGF-β1 stimulated normal renal tubular kidney epithelial cell model were established. In UUO model, administration of 50 mg kg-1 day-1 CTS markedly decreased the occurrence of kidney injury and the accumulation of fibronectin and collagen-1...
June 1, 2018: Oncotarget
https://www.readbyqxmd.com/read/29928446/small-interfering-rna-mediated-knockdown-of-fatty-acid-synthase-attenuates-the-proliferation-and-metastasis-of-human-gastric-cancer-cells-via-the-mtor-gli1-signaling-pathway
#5
Liang Sun, Yizhou Yao, Guofeng Pan, Shenghua Zhan, Weiqiang Shi, Ting Lu, Jinfeng Yuan, Kangjun Tian, Linhua Jiang, Shiduo Song, Xinguo Zhu, Songbing He
Fatty acid synthase (FASN), the main enzyme involved in de novo lipogenesis, is overexpressed in several types of tumor tissues. In addition, it is associated with tumor cell proliferation, metastasis, epithelial-mesenchymal transition (EMT) and a poor prognosis. However, the precise functions and internal mechanisms of FASN with regard to the proliferation, metastasis and EMT in gastric cancer (GC) cells remain elusive. The present study investigated FASN protein expression in 18 randomly selected pairs of GC tumors and matched normal tissues by western blot analysis...
July 2018: Oncology Letters
https://www.readbyqxmd.com/read/29928388/mir-885-5p-upregulation-promotes-colorectal-cancer-cell-proliferation-and-migration-by-targeting-suppressor-of-cytokine-signaling
#6
Meng Su, Baoli Qin, Fang Liu, Yuze Chen, Rui Zhang
The aim of the present study was to investigate the role of microRNA (miR)-885-5p in colorectal cancer cell proliferation and migration, and to determine the possible underlying molecular mechanisms. The expression of miR-885-5p in colorectal cancer tissue and cells was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression levels of three suppressor of cytokine signaling (SOCS) factors were detected by RT-qPCR and western blotting. The effects of miR-885-5p on tumor cell proliferation and migration were studied using MTT and Transwell assays, respectively...
July 2018: Oncology Letters
https://www.readbyqxmd.com/read/29928347/hiwi-overexpression-does-not-affect-proliferation-migration-or-apoptosis-of-liver-cancer-cells-in-vitro-or-in-vivo
#7
Hao Li, Chen-Xi Shi, Hui Liu, Hai-Han Zhang, Huai-Ming Sang, Muhammad-Djaleel Soyfoo, Jiu-Liang Cao, Shun-Fu Xu, Jian-Xia Jiang
Piwi like RNA-mediated gene silencing 1 (Hiwi) is a human homolog of the Piwi gene family that has been reported to be upregulated in hepatocellular carcinoma (HCC). The present study aimed to investigate the role of Hiwi in the initiation and development of HCC in vitro and in vivo . Adenovirus-mediated Hiwi overexpression was established in primary murine hepatocytes and SMMC7721 HCC cells. Cell viability and proliferation were assessed using MTT and EdU assays, respectively. Cell migration was measured using a scratch migration assay...
June 2018: Oncology Letters
https://www.readbyqxmd.com/read/29928144/expression-and-clinical-significance-of-autophagic-protein-lc3b-and-emt-markers-in-gastric-cancer
#8
Juan-Yi Wang, Tao Wu, Wu Ma, Shuo Li, Wen-Jiang Jing, Jun Ma, Dong-Mei Chen, Xiao-Jie Guo, Ke-Jun Nan
Background and aim: Gastric cancer (GC) is a fatal malignancy with high rate of recurrence and metastasis. Here, we investigated the correlations between the expression of autophagic protein LC3B and 2 epithelial-mesenchymal transition-related proteins (E-cadherin and Vimentin) and the clinicopathological factors and prognosis of patients with GC. Materials and methods: The expression of LC3B, E-cadherin, and Vimentin in GC samples (110 cases) and paracarcinoma tissues (40 cases) was analyzed using the Oncomine databases and further detected by immunohistochemistry...
2018: Cancer Management and Research
https://www.readbyqxmd.com/read/29928062/novel-antibody-reagents-for-characterization-of-drug-and-tumor-microenvironment-induced-changes-in-epithelial-mesenchymal-transition-and-cancer-stem-cells
#9
Tony Navas, Thomas D Pfister, Simona Colantonio, Amina Aziz, Lynda Dieckman, Richard G Saul, Jan Kaczmarczyk, Suzanne Borgel, Sergio Y Alcoser, Melinda G Hollingshead, Young H Lee, Donald P Bottaro, Tara Hiltke, Gordon Whiteley, Naoko Takebe, Robert J Kinders, Ralph E Parchment, Joseph E Tomaszewski, James H Doroshow
The presence of cancer stem cells (CSCs) and the induction of epithelial-to-mesenchymal transition (EMT) in tumors are associated with tumor aggressiveness, metastasis, drug resistance, and poor prognosis, necessitating the development of reagents for unambiguous detection of CSC- and EMT-associated proteins in tumor specimens. To this end, we generated novel antibodies to EMT- and CSC-associated proteins, including Goosecoid, Sox9, Slug, Snail, and CD133. Importantly, unlike several widely used antibodies to CD133, the anti-CD133 antibodies we generated recognize epitopes distal to known glycosylation sites, enabling analyses that are not confounded by differences in CD133 glycosylation...
2018: PloS One
https://www.readbyqxmd.com/read/29926241/circulating-tumor-cell-phenotype-indicates-poor-survival-and-recurrence-after-surgery-for-hepatocellular-carcinoma
#10
Huohui Ou, Yu Huang, Leyang Xiang, Zhanjun Chen, Yinghao Fang, Yixiong Lin, Zhonglin Cui, Sheng Yu, Xianghong Li, Dinghua Yang
BACKGROUND: Circulating tumors cells (CTCs) may be a promising prognostic marker for patients with malignant tumors. However, there are few reports regarding its value for hepatocellular carcinoma (HCC) patients. AIMS: To investigate CTCs with epithelial and mesenchymal phenotypes as a potential prognostic biomarker for HCC patients. METHODS: Peripheral blood samples were obtained from 165 HCC patients before radical surgery. CTCs were isolated via the CanPatrol CTC enrichment technique and classified using epithelial-mesenchymal transition (EMT) markers...
June 21, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29926178/dasatinib-suppresses-tgf%C3%AE-mediated-epithelial-mesenchymal-transition-in-alveolar-epithelial-cells-and-inhibits-pulmonary-fibrosis
#11
Ryota Kanemaru, Fumiyuki Takahashi, Motoyasu Kato, Yoichiro Mitsuishi, Ken Tajima, Hiroaki Ihara, Moulid Hidayat, Aditya Wirawan, Yoshika Koinuma, Daisuke Hayakawa, Shigehiro Yagishita, Ryo Ko, Tadashi Sato, Norihiro Harada, Yuzo Kodama, Fariz Nurwidya, Shinichi Sasaki, Shin-Ichiro Niwa, Kazuhisa Takahashi
PURPOSE: Transforming growth factor β (TGFβ)-mediated epithelial-mesenchymal transition (EMT) of alveolar epithelial cells contributes to pulmonary fibrosis. Dasatinib (DAS), a potent and broad-spectrum tyrosine kinase inhibitor, has been widely studied as an anti-cancer agent. However, the therapeutic application of DAS for pulmonary fibrosis has not been clarified. Our purpose here is to investigate the effect of DAS on TGFβ1-induced EMT in human alveolar and bronchial epithelial cells in vitro and to evaluate the efficacy of DAS on lung fibrosis in vivo...
June 20, 2018: Lung
https://www.readbyqxmd.com/read/29925392/upregulation-of-the-emt-marker-vimentin-is-associated-with-poor-clinical-outcome-in-acute-myeloid-leukemia
#12
Sharon Wu, Yang Du, John Beckford, Houda Alachkar
BACKGROUND: Vimentin (VIM) is a type III intermediate filament that maintains cell integrity, and is involved in cell migration, motility and adhesion. When overexpressed in solid cancers, vimentin drives epithelial to mesenchymal transition (EMT) and ultimately, metastasis. The effects of its overexpression in AML are unclear. METHODS: In this study, we analyzed the TCGA data of 173 AML patients for which complete clinical and expression data were available. In this analysis, we assessed the association between VIM mRNA expression and patient's clinical and molecular characteristics including clinical outcome...
June 20, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29923255/microrna-30d-mediated-breast-cancer-invasion-migration-and-emt-by-targeting-klf11-and-activating-stat3-pathway
#13
Mingli Han, Yimeng Wang, Guangcheng Guo, Lin Li, Dongwei Dou, Xin Ge, Pengwei Lv, Fang Wang, Yuanting Gu
miR-30d has been shown to play pivotal roles in cancer development, and has the potential to act as a diagnostic biomarker and therapeutic target in breast cancer. However, the specific function and molecular mechanism of miR-30d in breast cancer cell growth and metastasis is still unknown. The present study seeks to shed light on the potential contribution of the MiR-30d-KLF-11-STAT3 pathway in breast cancer. The results revealed that miR-30d levels were markedly increased in the breast cancer cell lines BT474, MDA-MB-231, HCC197, and MDA-MB-468 compared with the non-tumor mammary gland MCF10A cell line...
June 19, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29923214/microrna-183-induces-epithelial-mesenchymal-transition-and-promotes-endometrial-cancer-cell-migration-and-invasion-in-by-targeting-cpeb1
#14
Hanzhen Xiong, Ruichao Chen, Shaoyan Liu, Qiongyan Lin, Hui Chen, Qingping Jiang
The aim of this study is to evaluate the ability of microRNA-183 (miR-183) to influence epithelial-mesenchymal transition (EMT) and cell proliferation, migration, invasion, and apoptosis in endometrial cancer (EC) by targeting cytoplasmic polyadenylation element binding protein 1(CPEB1). EC tissues with matched nonmalignant tissues were collected from 208 EC patients. Ishikawa and RL95-2 cells were selected for cell experiments in vitro and each kind of cells were grouped into blank, negative control (NC), miR-183 mimic, miR-183 inhibitor, CPEB1 overexpression, and miR-183 mimic + CPEB1 overexpression groups...
June 20, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29923083/micrornas-in-regulation-of-triple-negative-breast-cancer-progression
#15
REVIEW
Dominika Piasecka, Marcin Braun, Radzislaw Kordek, Rafal Sadej, Hanna Romanska
PURPOSE: Dysregulation of miRNA profile has been associated with a broad spectrum of cellular processes underlying progression of various human malignancies. Increasing evidence suggests that specific microRNA clusters might be of clinical utility, especially in triple-negative breast carcinoma (TNBC), devoid of both predictive markers and potential therapeutic targets. Here we provide a comprehensive review of the existing data on microRNAs in TNBC, their molecular targets, a putative role in invasive progression with a particular emphasis on the epithelial-to-mesenchymal transition (EMT) and acquisition of stem-cell properties (CSC), regarded both as prerequisites for metastasis, and significance for therapy...
June 19, 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29921693/epithelial-mesenchymal-transition-in-human-prostate-cancer-demonstrates-enhanced-immune-evasion-marked-by-ido1-expression
#16
Kimberley Kolijn, Esther I Verhoef, Marcel Smid, René Böttcher, Guido W Jenster, Reno Debets, Geert Jlh van Leenders
Cancer invasion and metastasis are driven by epithelial-to-mesenchymal transition (EMT), yet the exact mechanisms that account for EMT in clinical prostate cancer are not fully understood. Expression of N-cadherin is considered a hallmark of EMT in clinical prostate cancer. In this study, we determined the molecular mechanisms associated with N-cadherin expression in prostate cancer patients. We performed laser capture micro-dissection of matched N-cadherin-positive and -negative prostate cancer areas from patient samples (n=8) followed by RNA sequencing...
June 19, 2018: Cancer Research
https://www.readbyqxmd.com/read/29921496/cancer-stemness-immune-cells-and-epithelial-mesenchymal-transition-cooperatively-predict-prognosis-in-colorectal-carcinoma
#17
Fangying Xu, Si Li, Jing Zhang, Lili Wang, Xuesong Wu, Jing Wang, Qiong Huang, Maode Lai
BACKGROUND: Tumor tissues consist of heterogeneous cancer cells and stroma cells, including cancer stem cells and immune cells. Epithelial-mesenchymal transition (EMT) programs closely associate with acquisition of stemness. We investigated for the first time the clinical significance of combining cancer stem cells, immune cells, and EMT traits. MATERIALS AND METHODS: In 419 colorectal carcinomas, stem-cell markers (Nanog, Lgr5, CD44v6, ALDH1A1), EMT markers (E-cadherin, Snail), and immune-cell markers (CD3+ , CD4+ or CD8+ T lymphocytes, CD20+ B lymphocytes, CD68+ macrophages) were detected in tumor center (TC) and tumor invasive front by an immunohistochemical method...
May 26, 2018: Clinical Colorectal Cancer
https://www.readbyqxmd.com/read/29921293/chip-functions-as-an-oncogene-by-promoting-colorectal-cancer-metastasis-via-activation-of-mapk-and-akt-signaling-and-suppression-of-e-cadherin
#18
Jingjing Xu, Jun Zhou, Hanjue Dai, Fei Liu, Wenjing Li, Wenjuan Wang, Feng Guo
BACKGROUND: The carboxyl terminus of Hsc70-interacting protein (CHIP) is an E3 ubiquitin ligase that plays a controversial role in different cancers, either as a tumor suppressor or a tumor promoter. To date, the exact function and underlying mechanism of CHIP in colorectal cancer (CRC) is not yet clear. Here we aimed to determine whether CHIP could affect the biological behaviors of CRC cells and its underlying mechanisms. METHODS: Stably transfected CHIP overexpression and depletion DLD-1 and HT-29 cells were established using Lipofectamine 2000...
June 19, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29921289/snai2-upregulation-is-associated-with-an-aggressive-phenotype-in-fulvestrant-resistant-breast-cancer-cells-and-is-an-indicator-of-poor-response-to-endocrine-therapy-in-estrogen-receptor-positive-metastatic-breast-cancer
#19
Carla L Alves, Daniel Elias, Maria B Lyng, Martin Bak, Henrik J Ditzel
BACKGROUND: Endocrine resistance in estrogen receptor-positive (ER+) breast cancer is a major clinical problem and is associated with accelerated cancer cell growth, increased motility and acquisition of mesenchymal characteristics. However, the specific molecules and pathways involved in these altered features remain to be detailed, and may be promising therapeutic targets to overcome endocrine resistance. METHODS: In the present study, we evaluated altered expression of epithelial-mesenchymal transition (EMT) regulators in ER+ breast cancer cell models of tamoxifen or fulvestrant resistance, by gene expression profiling...
June 19, 2018: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29920274/emt-subtype-influences-epithelial-plasticity-and-mode-of-cell-migration
#20
Nicole M Aiello, Ravikanth Maddipati, Robert J Norgard, David Balli, Jinyang Li, Salina Yuan, Taiji Yamazoe, Taylor Black, Amine Sahmoud, Emma E Furth, Dafna Bar-Sagi, Ben Z Stanger
Epithelial-mesenchymal transition (EMT) is strongly implicated in tumor cell invasion and metastasis. EMT is thought to be regulated primarily at the transcriptional level through the repressive activity of EMT transcription factors. However, these classical mechanisms have been parsed out almost exclusively in vitro, leaving questions about the programs driving EMT in physiological contexts. Here, using a lineage-labeled mouse model of pancreatic ductal adenocarcinoma to study EMT in vivo, we found that most tumors lose their epithelial phenotype through an alternative program involving protein internalization rather than transcriptional repression, resulting in a "partial EMT" phenotype...
June 18, 2018: Developmental Cell
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