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https://www.readbyqxmd.com/read/29053682/implantation-of-chronic-silicon-probes-and-recording-of-hippocampal-place-cells-in-an-enriched-treadmill-apparatus
#1
Anvar Sariev, Jinho Chung, Dajung Jung, Farnaz Sharif, Ju-Young Lee, Soyoun Kim, Sebastien Royer
An important requisite for understanding brain function is the identification of behavior and cell activity correlates. Silicon probes are advanced electrodes for large-scale electrophysiological recording of neuronal activity, but the procedures for their chronic implantation are still underdeveloped. The activity of hippocampal place cells is known to correlate with an animal's position in the environment, but the underlying mechanisms are still unclear. To investigate place cells, here we describe a set of techniques which range from the fabrication of devices for chronic silicon probe implants to the monitoring of place field activity in a cue-enriched treadmill apparatus...
October 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29052083/patient-specific-devices-and-population-level-evidence-evaluating-therapeutic-interventions-with-inherent-variation
#2
Mary Jean Walker
Designing and manufacturing medical devices for specific patients is becoming increasingly feasible with developments in 3D printing and 3D imaging software. This raises the question of how patient-specific devices can be evaluated, since our 'gold standard' method for evaluation, the randomised controlled trial (RCT), requires that an intervention is standardised across a number of individuals in an experimental group. I distinguish several senses of patient-specific device, and focus the discussion on understanding the problem of variations between instances of an intervention for RCT evaluation...
October 19, 2017: Medicine, Health Care, and Philosophy
https://www.readbyqxmd.com/read/29051121/preparation-and-investigation-of-novel-gastro-floating-tablets-with-3d-extrusion-based-printing
#3
Qijun Li, Xiaoying Guan, Mengsuo Cui, Zhihong Zhu, Kai Chen, Haoyang Wen, Danyang Jia, Jian Hou, Wenting Xu, Xinggang Yang, Weisan Pan
Three dimensional (3D) extrusion-based printing is a paste-based rapid prototyping process, which is capable of building complex 3D structures. The aim of this study was to explore the feasibility of 3D extrusion-based printing as a pharmaceutical manufacture technique for the fabrication of gastro-floating tablets. Novel low-density lattice internal structure gastro-floating tablets of dipyridamole were developed to prolong the gastric residence time in order to improve drug release rate and consequently, improve bioavailability and therapeutic efficacy...
October 16, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29050528/uv-assisted-3d-bioprinting-of-nano-reinforced-hybrid-cardiac-patch-for-myocardial-tissue-engineering
#4
Mohammad Izadifar, Dean Chapman, Paul Babyn, Xiongbiao Chen, Michael E Kelly
Biofabrication of cell supportive cardiac patches that can be directly implanted on myocardial infarct is a potential solution for myocardial infarction repair. Ideally, cardiac patches should be able to mimic myocardium extracellular matrix for rapid integration with the host tissue, raising the need to develop cardiac constructs with complex features. In particular, cardiac patches should be electrically conductive, mechanically robust and elastic, biologically active and pre-vascularized.. In this study, we aim to biofabricate a nano-reinforced hybrid cardiac patch laden with human coronary artery endothelial cells (HCAECs) with improved electrical, mechanical and biological behavior...
October 19, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29050180/-meta-analysis-of-three-dimensional-printing-patient-specific-instrumentation-versus-conventional-instrumentation-in-total-knee-arthroplasty
#5
J T Ren, C Xu, J S Wang, X L Liu
Objective: To evaluate the effects of three-dimensional printing patient-specific instrumentation(PSI) versus conventional instrumentation(CI) in the total knee arthroplasty. Methods: According to "patient-specific" , "patient-matched" , "custom" , "Instrumentation" , "Guide Instrumentation" , "cutting blocks" , "total knee arthroplasty" , "total knee replacement" , "TKA" and "TKR" , the literature on PubMed, EMbase, Cochrane library, CBM and WanFang were searched. According to the inclusion and exclusion criteria, the high quality randomized control trial (RCT) studies about three-dimensional (3D) printing patient-specific instrumentation versus conventional instrumentation in the total knee arthroplasty were collected...
October 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/29049947/3d-spongy-graphene-modified-screen-printed-sensors-for-the-voltammetric-determination-of-the-narcotic-drug-codeine
#6
Mona A Mohamed, Dalia M El-Gendy, Nashaat Ahmed, Craig E Banks, Nageh K Allam
Adenine-functionalized spongy graphene (FSG) composite, fabricated via a facile and green synthetic method, has been explored as a potential electrocatalyst toward the electroanalytical sensing of codeine phosphate (COD). The synthesized composite is characterized using Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray powder diffraction, UV-vis absorption spectroscopy, scanning electron microscopy, high resolution transmission electron microscopy (HRTEM), and thermogravimetric analysis...
October 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29049565/a-low-cost-bioprosthetic-semilunar-valve-for-research-disease-modelling-and-surgical-training-applications
#7
Benoit Rosa, Zurab Machaidze, Borami Shin, Sunil Manjila, David W Brown, Christopher W Baird, John E Mayer, Pierre E Dupont
OBJECTIVES: This paper provides detailed instructions for constructing low-cost bioprosthetic semilunar valves for animal research and clinical training. This work fills an important gap between existing simulator training valves and clinical valves by providing fully functioning designs that can be employed in ex vivo and in vivo experiments and can also be modified to model valvular disease. METHODS: Valves are constructed in 4 steps consisting of creating a metal frame, covering it with fabric and attaching a suture ring and leaflets...
August 2, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29049538/3d-printed-mitral-valve-models-affordable-simulation-for-robotic-mitral-valve-repair
#8
Ned Premyodhin, Divneet Mandair, Alice S Ferng, Timothy S Leach, Ryan P Palsma, Mohammad Z Albanna, Zain I Khalpey
OBJECTIVES: 3D printed mitral valve (MV) models that capture the suture response of real tissue may be utilized as surgical training tools. Leveraging clinical imaging modalities, 3D computerized modelling and 3D printing technology to produce affordable models complements currently available virtual simulators and paves the way for patient- and pathology-specific preoperative rehearsal. METHODS: We used polyvinyl alcohol, a dissolvable thermoplastic, to 3D print moulds that were casted with liquid platinum-cure silicone yielding flexible, low-cost MV models capable of simulating valvular tissue...
July 31, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29049280/ethoscopes-an-open-platform-for-high-throughput-ethomics
#9
Quentin Geissmann, Luis Garcia Rodriguez, Esteban J Beckwith, Alice S French, Arian R Jamasb, Giorgio F Gilestro
Here, we present the use of ethoscopes, which are machines for high-throughput analysis of behavior in Drosophila and other animals. Ethoscopes provide a software and hardware solution that is reproducible and easily scalable. They perform, in real-time, tracking and profiling of behavior by using a supervised machine learning algorithm, are able to deliver behaviorally triggered stimuli to flies in a feedback-loop mode, and are highly customizable and open source. Ethoscopes can be built easily by using 3D printing technology and rely on Raspberry Pi microcomputers and Arduino boards to provide affordable and flexible hardware...
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/29048347/three-dimensional-3d-printing-of-polymer-metal-hybrid-materials-by-fused-deposition-modeling
#10
Susanna Fafenrot, Nils Grimmelsmann, Martin Wortmann, Andrea Ehrmann
Fused deposition modeling (FDM) is a three-dimensional (3D) printing technology that is usually performed with polymers that are molten in a printer nozzle and placed line by line on the printing bed or the previous layer, respectively. Nowadays, hybrid materials combining polymers with functional materials are also commercially available. Especially combinations of polymers with metal particles result in printed objects with interesting optical and mechanical properties. The mechanical properties of objects printed with two of these metal-polymer blends were compared to common poly (lactide acid) (PLA) printed objects...
October 19, 2017: Materials
https://www.readbyqxmd.com/read/29048214/validation-of-a-full-immersion-simulation-platform-for-percutaneous-nephrolithotomy-using-3d-printing-technology
#11
Ahmed Ghazi, Timothy Campbell, Rachel Melnyk, Changyong Feng, Alex Andrusco, Jonathan Stone, Erdal Erturk
INTRODUCTION AND OBJECTIVES: The restriction of resident hours with an increasing focus on patient safety and a reduced caseload has impacted surgical training. A complex and complication prone procedure such as percutaneous nephrolithotomy (PCNL) with a steep learning curve may create an unsafe environment for hands-on resident training. In this study, we validate a high fidelity, inanimate PCNL model within a full immersion simulation environment. METHODS: Anatomically correct models of the human pelvicalyceal system (PCS), kidney, and relevant adjacent structures were created using poly-vinyl alcohol (PVA) hydrogels and 3D-printed injection molds...
October 19, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/29048159/using-printing-orientation-for-tuning-fluidic-behaviour-in-microfluidic-chips-made-by-fused-deposition-modelling-fdm-3d-printing
#12
Feng Li, Niall P Macdonald, Rosanne M Guijt, Michael C Breadmore
Fluidic behaviour in microfluidic devices is dictated by low Reynolds numbers, complicating mixing. Here, the effect of the orientation of the extruded filament on the fluidic behaviour is investigated in fused deposition modeling (FDM) printed fluidic devices. Devices were printed with filament orientations at 0°, 30°, 60°, 90° to the direction of the flow. The extent of mixing was observed when pumping yellow and blue solutions into the inlets of a y-shaped device, and measuring the extent of mixing of two coloured solutions under different angles and at flow rates of 25, 50, and 100 μL/min...
October 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29048055/automated-batch-characterization-of-inkjet-printed-elastomer-lenses-using-a-lego-platform
#13
Yu-Lung Sung, Jacob Garan, Hoang Nguyen, Zhenyu Hu, Wei-Chuan Shih
Small, self-adhesive, inkjet-printed elastomer lenses have enabled smartphone cameras to image and resolve microscopic objects. However, the performance of different lenses within a batch is affected by hard-to-control environmental variables. We present a cost-effective platform to perform automated batch characterization of 300 lens units simultaneously for quality inspection. The system was designed and configured with LEGO bricks, 3D printed parts, and a digital camera. The scheme presented here may become the basis of a high-throughput, in-line inspection tool for quality control purposes and can also be employed for optimization of the manufacturing process...
September 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/29047957/diffuse-optical-localization-of-blood-vessels-and-3d-printing-for-guiding-oral-surgery
#14
Brian Z Bentz, Timothy C Wu, Vaibhav Gaind, Kevin J Webb
Diffuse optical imaging through centimeters of tissue has emerged as a powerful tool in biomedical research. However, applications in the operating theater have been limited in part due to data set requirements and computational burden. We present an approach that uses a small number of optical source-detector pairs that allows for the fast localization of arteries in the roof of the mouth and has the potential to reduce complications during oral surgery. The arteries are modeled as multiple-point absorbers, allowing localization of their complex shapes...
August 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/29047500/step-by-step-guide-to-reduce-spatial-coherence-of-laser-light-using-a-rotating-ground-glass-diffuser
#15
Tim Stangner, Hanqing Zhang, Tobias Dahlberg, Krister Wiklund, Magnus Andersson
Wide field-of-view imaging of fast processes in a microscope requires high light intensities motivating the use of lasers as light sources. However, due to their long spatial coherence length, lasers are inappropriate for such applications, as they produce coherent noise and parasitic reflections, such as speckle, degrading image quality. Therefore, we provide a step-by-step guide for constructing a speckle-free and high-contrast laser illumination setup using a rotating ground glass diffuser driven by a stepper motor...
July 1, 2017: Applied Optics
https://www.readbyqxmd.com/read/29047370/the-recent-development-and-applications-of-fluidic-channels-by-3d-printing
#16
REVIEW
Yufeng Zhou
The technology of "Lab-on-a-Chip" allows the synthesis and analysis of chemicals and biological substance within a portable or handheld device. The 3D printed structures enable precise control of various geometries. The combination of these two technologies in recent years makes a significant progress. The current approaches of 3D printing, such as stereolithography, polyjet, and fused deposition modeling, are introduced. Their manufacture specifications, such as surface roughness, resolution, replication fidelity, cost, and fabrication time, are compared with each other...
October 18, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/29046717/designing-of-pla-scaffolds-for-bone-tissue-replacement-fabricated-by-ordinary-commercial-3d-printer
#17
Aleš Gregor, Eva Filová, Martin Novák, Jakub Kronek, Hynek Chlup, Matěj Buzgo, Veronika Blahnová, Věra Lukášová, Martin Bartoš, Alois Nečas, Jan Hošek
BACKGROUND: The primary objective of Tissue engineering is a regeneration or replacement of tissues or organs damaged by disease, injury, or congenital anomalies. At present, Tissue engineering repairs damaged tissues and organs with artificial supporting structures called scaffolds. These are used for attachment and subsequent growth of appropriate cells. During the cell growth gradual biodegradation of the scaffold occurs and the final product is a new tissue with the desired shape and properties...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/29046689/an-updated-protocol-for-high-throughput-plant-tissue-sectioning
#18
Jonathan A Atkinson, Darren M Wells
Quantification of the tissue and cellular structure of plant material is essential for the study of a variety of plant sciences applications. Currently, many methods for sectioning plant material are either low throughput or involve free-hand sectioning which requires a significant amount of practice. Here, we present an updated method to provide rapid and high-quality cross sections, primarily of root tissue but which can also be readily applied to other tissues such as leaves or stems. To increase the throughput of traditional agarose embedding and sectioning, custom designed 3D printed molds were utilized to embed 5-15 roots in a block for sectioning in a single cut...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29044984/3d-printed-calcium-phosphate-and-poly-caprolactone-composites-with-improved-mechanical-properties-and-preserved-microstructure
#19
Joseph B Vella, Ryan P Trombetta, Michael D Hoffman, Jason Inzana, Hani Awad, Danielle S W Benoit
Biphasic calcium phosphate scaffolds formed via 3D printing technology to exhibit porosity and chemical resorbability to promote osseointegration often lack the strength and toughness required to withstand loading in bone tissue engineering applications. Herein, sintering and CaP:poly(caprolactone) (PCL) composite formation were explored to improve 3D printed scaffold strength and toughness. Hydroxyapatite and α-tricalcium phosphate (α-TCP) biphasic calcium powders were printed using phosphoric acid binder, which generated monetite and hydroxyapatite scaffolds...
October 16, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29044894/immediate-implant-placement-and-provisionalization-following-tumor-resective-surgery-in-the-midfacial-region-a-case-series
#20
Nelson Fernandes, Jacobus van den Heever, Leanne Sykes
Surgical and prosthodontic restoration of the midfacial region following tumor resection has always posed a considerable challenge, as this area serves crucial functional and esthetic roles. Being diagnosed and subsequently treated for facial tumors can have an immense psychosocial impact on a patient, as the resulting defects are often disfiguring, and lead to an inability to masticate, swallow, and speak clearly. Provision of an immediate facial and dental prosthesis at the time of surgery can limit these side effects and help reduce mental duress on these patients and their families, as well as aid in the process of rehabilitation...
October 18, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
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