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Weiyi Wang, Ruixuan Chen, Zhuhua Luo, Wei Wang, Jianming Chen
Two new benzoate derivatives, ethyl 3,5-dimethoxy-2-propionylbenzoate (1) and ethyl 3,5-dihydroxy-2-propionylbenzoate (2), and one new phenylacetate derivative, ethyl 3,5-dimethoxy-2-propionylphenylacetate (3), together with 9 known compounds, were isolated from the fermentation of Engyodontium album derived from deep sea sediment. Their structures were elucidated by spectroscopic techniques, such as NMR, IR and HRESIMS. Compound 3 exhibited inhibitory activities against methicillin-resistant Staphylococcus aureus ATCC 43300 (MRSA) and Vibrio vulnificus, with MIC values of 7...
October 18, 2016: Natural Product Research
Mai Yamamoto, Takashige Kashimoto, Yukihiro Yoshimura, Nao Tachibana, Shiho Kuroda, Yoshiko Miki, Sou Kitabayashi, Ping Tong, Jianbo Xiao, Koichi Tanaka, Hiroshi Hamamoto, Kazuhisa Sekimizu, Koichiro Yamamoto
The halophilic marine bacterium, Vibrio vulnificus, occasionally causes fatal septicemia in immunocompromised patients. Mice are commonly used as experimental animals to investigate the virulence of V. vulnificus, however, a large number of mice are generally required for bioassays. The present study examined whether the invertebrate species, silkworms, can be used instead of mice to investigate V. vulnificus virulence. When the silkworms were inoculated with 1.2x107 colony forming units of V. vulnificus OPU1‑Rf, a virulent strain of V...
September 26, 2016: Molecular Medicine Reports
H W Kim, Y J Hong, J I Jo, S D Ha, S H Kim, H J Lee, M S Rhee
Microbiological quality of 206 raw ready-to-eat seafood samples were investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster, and squid) and distribution channels (fishery, hyper, and online market). Enumeration of Aerobic Plate Count (APC) and total coliforms (TC) and pathogenic bacteria (Bacillus cereus, Staphylococcus aureus, and Vibrio parahaemolyticus) were performed, and level of microbiological quality were classified into four groups: satisfactory, acceptable, unsatisfactory, and unacceptable...
October 16, 2016: Letters in Applied Microbiology
Guadalupe Barrera-Escorcia, Irma Wong-Chang, Carlos Leopoldo Fernández-Rendón, Alfonso Vázquez Botello, Bruno Gómez-Gil, Marcial Leonardo Lizárraga-Partida
Oysters can accumulate potentially pathogenic water bacteria. The objective of this study was to compare two procedures to quantify Vibrio species present in oysters to determine the most sensitive method. We analyzed oyster samples from the Gulf of Mexico, commercialized in Mexico City. The samples were inoculated in tubes with alkaline peptone water (APW), based on three tubes and four dilutions (10(-1) to 10(-4)). From these tubes, the first quantification of Vibrio species was performed (most probable number (MPN) from tubes) and bacteria were inoculated by streaking on thiosulfate-citrate-bile salts-sucrose (TCBS) petri dishes...
November 2016: Environmental Monitoring and Assessment
Kajal Chakraborty, Bini Thilakan, Rekha Devi Chakraborty, Vamshi Krishna Raola, Minju Joy
The brown seaweed, Sargassum myriocystum associated with heterotrophic bacterium, Bacillus subtilis MTCC 10407 (JF834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria Aeromonas hydrophila, Vibrio vulnificus, and Vibrio parahaemolyticus. B. subtilis MTCC 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. Using bioassay-guided fractionation, two new antibacterial O-heterocyclic compounds belonging to pyranyl benzoate analogs of polyketide origin, with activity against pathogenic bacteria, have been isolated from the ethyl acetate extract of B...
September 13, 2016: Applied Microbiology and Biotechnology
Craig Baker-Austin, James D Oliver
No abstract text is available yet for this article.
2016: IDCases
Antonio Benítez-Cabello, Joaquín Bautista-Gallego, Antonio Garrido-Fernández, Kalliopi Rantsiou, Luca Cocolin, Rufino Jiménez-Díaz, Francisco N Arroyo-López
This paper describes the dominant bacterial species metabolically active through the industrial production of Spanish-style Manzanilla and Gordal olives. For this purpose, samples (brines and fruits) obtained at 0, 15, and 90 fermentation days were analyzed by a culture-independent approach to determine viable cells by reverse transcription of RNA and further PCR-DGGE analysis, detecting at least 7 different species. Vibrio vulnificus, Lactobacillus plantarum group, and Lactobacillus parafarraginis were present in samples from both cultivars; Lactobacillus sanfranciscensis and Halolactobacillus halophilus were detected only in Gordal samples, while Staphylococcus sp...
2016: Frontiers in Microbiology
Yong Liu, Ning-Qiu Li, Xin-Peng Zhao, Bin Yue, Shu-Wen He, Zhi-Xin Gao, Shun Zhou, Min Zhang
C-type lectins (CTLs) are important pattern recognition receptors (PRRs) that play vital roles in innate immunity. In teleosts, a number of CTLs have been reported, but their in vivo effects on host defense are still limited. In this study, a CTL homolog (SsLec1) was identified from black rockfish, Sebastes schlegelii, and its structure, expression and biological function was analyzed. The open reading frame of SsLec1 is 633 bp, with a 5'- untranslated region (UTR) of 36 bp and a 3'- UTR of 117 bp. The deduced amino acid sequence of SsLec1 shares the highest overall identity (73...
October 2016: Fish & Shellfish Immunology
Zhiyong Guo, Yuhong Sha, Yufang Hu, Zhongqing Yu, Yingying Tao, Yanjie Wu, Min Zeng, Sui Wang, Xing Li, Jun Zhou, Xiurong Su
A novel Faraday cage-type electrochemiluminescence (ECL) immunosensor devoted to the detection of Vibrio vulnificus (VV) was fabricated. The sensing strategy was presented by a unique Faraday cage-type immunocomplex based on immunomagnetic beads (IMBs) and multi-functionalized graphene oxide (GO) labeled with (2,2'-bipyridine)(5-aminophenanthroline)ruthenium (Ru-NH2). The multi-functionalized GO could sit on the electrode surface directly due to the large surface area, abundant functional groups, and good electronic transport property...
October 2016: Analytical and Bioanalytical Chemistry
M Madiyal, V K Eshwara, I Halim, W Stanley, M Prabhu, C Mukhopadhyay
Necrotising fasciitis is one of the fatal skin and soft tissue infections. Vibrio vulnificus is a rare cause of necrotising fasciitis; however, the disease is one of the major manifestations of the bacteria. Here, we report one such case in a middle-aged male patient. He presented with the signs of bilateral lower limb cellulitis and altered sensorium. V. vulnificus was isolated from blood culture and also from debrided tissue. Though the organism is well characterised, it is a rare causative agent of necrotising fasciitis...
July 2016: Indian Journal of Medical Microbiology
So-Young Kim, Boram Chung, Jin-Hee Chang, Gyoo Yeol Jung, Hyoun Wook Kim, Beom-Young Park, Sang Suk Oh, Mi-Hwa Oh
Capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) coupled with stuffer-free multiplex ligation-dependent probe amplification (MLPA) was developed to identify 13 species of foodborne pathogens simultaneously. Species-specific MLPA probes were designed for nine of these species. These probes were targeted to the groEL, glyA, MMS, tuf, inv, ipaH, nuc, vvh, and 16S rRNA genes, which corresponded to Bacillus cereus, Campylobacter coli, Cronobacter sakazakii, Enterococcus spp., Salmonella spp...
October 2016: Foodborne Pathogens and Disease
Jin Hwan Park, Byungho Lee, Youmi Jo, Sang Ho Choi
Biofilms are recalcitrant and raise safety problems in the food industry. In this study, the role of CabA, an extracellular matrix protein, in the resistance of the biofilms of Vibrio vulnificus, a foodborne pathogen, to decontamination strategies was investigated. Biofilms of the cabA mutant revealed reduced resistance to detachment by vibration and disinfection by sodium hypochlorite compared to the biofilms of the parental wild type in vitro. The reduced resistance of the cabA mutant biofilms was complemented by introducing a recombinant cabA, indicating that the reduced resistance of the cabA mutant biofilms is caused by the inactivation of cabA...
November 7, 2016: International Journal of Food Microbiology
Fabini D Orata, Yue Xu, Lori M Gladney, Lavanya Rishishwar, Rebecca J Case, Yan Boucher, I King Jordan, Cheryl L Tarr
Four Vibrio spp. isolates from the historical culture collection at the Centers for Disease Control and Prevention, obtained from human blood specimens (n = 3) and river water (n = 1), show characteristics distinct from those of isolates of the most closely related species, Vibrio navarrensis and Vibrio vulnificus, based on phenotypic and genotypic tests. They are specifically adapted to survival in both freshwater and seawater, being able to grow in rich media without added salts as well as salinities above that of seawater...
July 27, 2016: International Journal of Systematic and Evolutionary Microbiology
Shun Zhou, Zhi-Xin Gao, Min Zhang, Dan-Yang Liu, Xin-Peng Zhao, Yong Liu
A quadruplex loop-mediated isothermal amplification (LAMP) method was developed to detect four Vibrio species, including Vibrio ichthyoenteri, Vibrio parahaemolyticus, Vibrio scophthalmi, and Vibrio vulnificus, simultaneously. Four sets of species-specific primers were designed with different restriction sites contained in the inner primers. The quadruplex LAMP method could distinguish four Vibrio species via the subsequent restriction enzyme analysis. The sensitivity of the quadruplex LAMP method were 10(2)-10(3) times higher than the sensitivity of conventional PCR...
2016: SpringerPlus
Amel Ismail, Leila Ktari, Mehboob Ahmed, Henk Bolhuis, Abdellatif Boudabbous, Lucas J Stal, Mariana Silvia Cretoiu, Monia El Bour
Macroalgae belonging to the genus Padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. Hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. Here we report antibacterial activities of epiphytic bacteria isolated from Padina pavonica (Peacocks tail) located on northern coast of Tunisia. Eighteen isolates were obtained in pure culture and tested for antimicrobial activities...
2016: Frontiers in Microbiology
Simone M Raszl, Brett A Froelich, Cleide R W Vieira, Denene A Blackwood, Rachel T Noble
The bacterial species, Vibrio parahaemolyticus and V. vulnificus, are ubiquitous in estuaries and coastal waters throughout the world, but they also happen to be important human pathogens. They are concentrated by filter feeding shellfish which are often consumed raw or undercooked, providing an important potential route of entry for an infective dose of these bacteria. V. parahaemolyticus can cause abdominal cramping, nausea, diarrhea, vomiting, chills, and fever. V. vulnificus can cause similar gastrointestinal-related symptoms, but can also spread to the bloodstream, resulting in primary septicemia, and it can also cause disease via wound infections...
July 27, 2016: Journal of Applied Microbiology
Byung Cheol Lee, Arim Lee, Jee Hyung Jung, Sang Ho Choi, Tae Sung Kim
Vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. In the present study, the antimicrobial activity of natural marine product-derived compounds against V. vulnificus, were investigated in vitro and in vivo. Twelve pure compounds were isolated from natural marine products and their inhibitory effects on V. vulnificus-induced cytotoxicity were determined in INT‑407 cells. Among the 12 pure compounds tested, treatment with psammaplin A significantly suppressed V...
September 2016: Molecular Medicine Reports
Andrew E Armitage, Pei Jin Lim, Joe N Frost, Sant-Rayn Pasricha, Elizabeth J Soilleux, Emma Evans, Alireza Morovat, Ana Santos, Rebeca Diaz, Daniel Biggs, Benjamin Davies, Uzi Gileadi, Peter A Robbins, Samira Lakhal-Littleton, Hal Drakesmith
Withdrawal of iron from serum (hypoferraemia) is a conserved innate immune antimicrobial strategy that can withhold this critical nutrient from invading pathogens, impairing their growth. Hepcidin (Hamp1) is the master regulator of iron and its expression is induced by inflammation. Mice lacking Hamp1 from birth rapidly accumulate iron and are susceptible to infection by blood-dwelling siderophilic bacteria such as Vibrio vulnificus. In order to study the innate immune role of hepcidin against a background of normal iron status, we developed a transgenic mouse model of tamoxifen-sensitive conditional Hamp1 deletion (termed iHamp1-KO mice)...
2016: Journal of Innate Immunity
David Pajuelo, Carla Hernández-Cabanyero, Eva Sanjuan, Chung-Te Lee, Francisco Xavier Silva-Hernández, Lien-I Hor, Simon MacKenzie, Carmen Amaro
In this study, we aimed to analyze the global response to iron in the broad-range host pathogen Vibrio vulnificus under the hypothesis that iron is one of the main signals triggering survival mechanisms both inside and outside its hosts. To this end, we selected a strain from the main zoonotic clonal-complex, obtained a mutant in the ferric-uptakeregulator (Fur), and analyzed their transcriptomic profiles in both iron-excess and ironpoor conditions by using a strain-specific microarray platform. Among the genes differentially expressed, we identified around 250 as putatively involved in virulence and survival-related mechanisms...
June 27, 2016: Environmental Microbiology
Han Young Chung, You-Tae Kim, Suyeon Kim, Eun Jung Na, Hye-Jin Ku, Keun Hwa Lee, Sang Taek Heo, Sangryeol Ryu, Heebal Kim, Sang Ho Choi, Ju-Hoon Lee
BACKGROUND: Vibrio vulnificus, a resident in the human gut, is frequently found in seafood, causing food-borne illnesses including gastroenteritis and severe septicemia. While V. vulnificus has been known to be one of the major food-borne pathogens, pathogenicity and virulence factors are not fully understood yet. To extend our understanding of the pathogenesis of V. vulnificus at the genomic level, the genome of V. vulnificus FORC_017 isolated from a female patient experiencing a hemorrhagic rash was completely sequenced and analyzed...
2016: Gut Pathogens
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