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https://www.readbyqxmd.com/read/29295912/role-of-heat-shock-proteases-in-quorum-sensing-mediated-regulation-of-biofilm-formation-by-vibrio-species
#1
Kyung-Jo Lee, You-Chul Jung, Soon-Jung Park, Kyu-Ho Lee
Capsular polysaccharide (CPS) is essential for the dispersal of biofilms formed by the pathogenic bacterium Vibrio vulnificus CPS production is induced by the quorum-sensing (QS) master regulator SmcR when biofilms mature. However, V. vulnificus biofilms formed under heat shock conditions did not exhibit the dispersion stage. Transcripts of the CPS gene cluster were at basal levels in the heat-exposed cell owing to reduced cellular levels of SmcR. At least two proteases induced by heat shock, ClpPA and Lon, were responsible for determining the instability of SmcR...
January 2, 2018: MBio
https://www.readbyqxmd.com/read/29282420/rapid-identification-of-pathogens-responsible-for-necrotizing-fasciitis-on-an-integrated-microfluidic-system
#2
Ju-Ching Yu, Pang-Hsin Hsieh, Hsing-Wen Tsai, Wen-Hsin Chang, Ting-Hang Liu, Mel S Lee, Kuo-Ti Peng, Kuo-Chin Huang, Gwo-Bin Lee
Necrotic fasciitis (NF) is a particularly aggressive and serious infection of the fascia that can penetrate into the musculature and internal organs, resulting in death if not treated promptly. In this work, an integrated microfluidic system composed of micropumps, microvalves, and micromixers was used to automate the detection of pathogens associated with NF. The entire molecular diagnostic process, including bacteria isolation, lysis, nucleic acid amplification and optical detection steps, was enacted on this developed system...
November 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29261778/functional-implications-of-hexameric-assembly-of-rraa-proteins-from-vibrio-vulnificus
#3
Saemee Song, Seokho Hong, Jinyang Jang, Ji-Hyun Yeom, Nohra Park, Jaejin Lee, Yeri Lim, Jun-Yeong Jeon, Hyung-Kyoon Choi, Minho Lee, Nam-Chul Ha, Kangseok Lee
RNase E has a pivotal role in the degradation and processing of RNAs in Escherichia coli, and protein inhibitors RraA and RraB control its enzymatic activity. The halophilic pathogenic bacterium Vibrio vulnificus also expresses orthologs of RNase E and RraA-RNase EV, RraAV1, and RraAV2 (herein renamed as VvRNase E, VvRraA1, and VvRraA2). A previous study showed that VvRraA1 actively inhibits the ribonucleolytic activity of VvRNase E by interacting with the C-terminal region of VvRNase E. However, the molecular mechanism underlying the effect of VvRraA1 on the ribonucleolytic activity of VvRNase E has not yet been elucidated...
2017: PloS One
https://www.readbyqxmd.com/read/29246810/a-new-l-type-lectin-lvltlc1-from-the-shrimp-litopenaeus-vannamei-facilitates-the-clearance-of-vibrio-harveyi
#4
Yushun Tian, Ting Chen, Wen Huang, Peng Luo, Da Huo, Long Yun, Chaoqun Hu, Yiming Cai
According to structures and functions of lectins found in shrimp, they are classified into seven types, namely, L-type, C-type, P-type, M-type, galectins, fibrinogen-like domain lectins, and calnexin/calreticulin. Until now, the researches of shrimp lectins are mainly focused on C-type lectins. In this study, we identified a new L-type lectin, designated as LvLTLC1, from the shrimp Litopenaeus vannamei. The cDNA of LvLTLC1 is 1184 bp with an open reading frame of 990 bp encoding a protein of 329 amino acids...
December 12, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29235706/a-conserved-tad-pilus-promotes-vibrio-vulnificus-oyster-colonization
#5
Meng Pu, Patrick Duriez, Mattan Arazi, Dean A Rowe-Magnus
Vibrio vulnificus has the highest death rate (>35%) and per-case economic burden ($3.3 million) of any foodborne pathogen in the US. Infections occur via open wounds or following ingestion of contaminated seafood, most infamously oysters. We isolated a 1000th generation descendant, designated NT, that exhibited increased biofilm and aggregate formation relative to its parent. We identified two significant causal changes underlying these NT phenotypes. First, the entire 24-kb capsular polysaccharide biosynthesis locus, which is essential for virulence but inhibits biofilm formation, had been purged from the genome...
December 13, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29208199/-amputation-of-an-arm-due-to-infection-with-vibrio-vulnificus-after-beach-holiday
#6
Esra Arici, Allan Evald, Dennis Back Holmgaard, Lars Quist, Susanne Dam Poulsen, Signe W Worm
Infection with Vibrio vulnificus is a rare condition with approximately 11 cases reported in the Danish literature. It is characterized by soft tissue infection/wound, necrotizing fasciitis and septicaemia. In this case report we present a patient admitted with a rapid progression and sepsis consistent with V. vulnificus infection but with no informa-tion of water exposure. The initial treatment was surgery and sepsis management including broad-spectrum antibiotics. On day eight the patient's right arm was amputated...
November 27, 2017: Ugeskrift for Laeger
https://www.readbyqxmd.com/read/29201149/complete-genome-sequence-of-the-vibrio-vulnificus-strain-vv2014djh-a-human-pathogenic-bacterium-isolated-from-a-death-case-in-china
#7
Junhang Pan, Yi Sun, Wenwu Yao, Haiyan Mao, Yanjun Zhang, Muyuan Zhu
Background: Vibrio vulnificus, an opportunistic pathogen, is the causative agent of life-threatening septicemia and severe wound infections. However, the pathogenicity and virulence factors of V. vulnificus are not fully understood. Here we report the complete genome sequence of V. vulnificus VV2014DJH, which was isolated from a death case. Results: The genome of the V. vulnificus VV2014DJH contains two circular chromosomes with a mean G+C content of 46.8%, but does not consists of any plasmids...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/29195979/analysis-of-the-bacterial-microbiome-in-the-small-octopus-octopus-variabilis-from-south-korea-to-detect-the-potential-risk-of-foodborne-illness-and-to-improve-product-management
#8
Jin-Jae Lee, Tae-Yoon Kim, Sang Ho Choi, Bong-Soo Kim
The small octopus (Octopus variabilis) is a popular seafood in many countries including South Korea. Because it is often consumed uncooked, the microorganisms in it often cause food poisoning. Therefore, analyzing the microbiome of the small octopus can help to understand the risk of food poisoning and manage octopus products better. A total of 40 small octopuses were collected from four sites in November and August. The microbiota was analyzed using Illumina Miseq sequencing, and the amount of bacteria was quantified by real-time PCR...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29183811/to17-a-teleost-antimicrobial-peptide-that-induces-degradation-of-bacterial-nucleic-acids-and-inhibits-bacterial-infection-in-red-drum-sciaenops-ocellatus
#9
Shu-Wen He, Guang-Hua Wang, Bin Yue, Shun Zhou, Min Zhang
Tissue factor pathway inhibitor (TFPI)-1 is well known for its role as an inhibitor of blood coagulation. Several studies have demonstrated that the C-terminal peptides of TFPI-1 are active against a broad spectrum of microorganisms. In a previous study, we found that TO17 (with 17 amino acids), a TFPI-1 C-terminal peptide from red drum (Sciaenops ocellatus), was active against Edwardsiella tarda. In the present study, we investigated further the antimicrobial spectrum, action mode, as well as the immunostimulatory property of TO17...
November 25, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29173069/direct-detection-of-vibrio-vulnificus-vibrio-parahaemolyticus-and-vibrio-alginolyticus-from-clinical-and-environmental-samples-by-a-multiplex-touchdown-polymerase-chain-reaction-assay
#10
Ji-Fang Yin, Ming-Yi Wang, Ying-Jian Chen, Hong-Qiu Yin, Yan Wang, Ming-Qiang Lin, Ai-Yan Liu, Cheng-Jin Hu
BACKGROUND: Vibrio vulnificus, V. alginolyticus, and V. parahaemolyticus are commonly and opportunistically pathogenic to humans. METHODS: In this study, a novel multiple touchdown polymerase chain reaction method (MT-PCR) was developed to benefit rapid and simultaneous detection of the presence of the three Vibrio species from the enriched clinical and environmental samples. RESULTS: The method showed a sensitivity of 104 colony forming units (CFU)/mL for V...
November 27, 2017: Surgical Infections
https://www.readbyqxmd.com/read/29168349/bioactive-pimarane-diterpenes-from-the-arctic-fungus-eutypella-sp-d-1
#11
Xiaoli Wang, Kunlai Sun, Bin Wang
Two new pimarane diterpenes, libertellenone M (1) and libertellenone N (2), together with five known compounds were isolated from the culture extract of Eutypella sp. D-1 derived from high-latitude soil of the Arctic. The structures of these compounds were determined by spectroscopic data as well as experimental and calculated electronic circular dichroism (ECD) analysis. Antimicrobial and cytotoxic activities of the isolated compounds were evaluated. Compound 3 exhibited weak antibacterial activity against Escherichia coli, Bacillus subtilis, and Vibrio vulnificus, each with MIC values of 16 μg/mL...
November 23, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29163452/the-opportunistic-pathogen-vibrio-vulnificus-produces-outer-membrane-vesicles-in-a-spatially-distinct-manner-related-to-capsular-polysaccharide
#12
Cheri M Hampton, Ricardo C Guerrero-Ferreira, Rachel E Storms, Jeannette V Taylor, Hong Yi, Paul A Gulig, Elizabeth R Wright
Vibrio vulnificus, a bacterial species that inhabits brackish waters, is an opportunistic pathogen of humans. V. vulnificus infections can cause acute gastroenteritis, invasive septicemia, tissue necrosis, and potentially death. Virulence factors associated with V. vulnificus include the capsular polysaccharide (CPS), lipopolysaccharide, flagellum, pili, and outer membrane vesicles (OMVs). The aims of this study were to characterize the morphology of V. vulnificus cells and the formation and arrangement of OMVs using cryo-electron microscopy (cryo-EM)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29150510/characterization-of-vibrio-parahaemolyticus-and-vibrio-vulnificus-recovered-from-oysters-during-an-oyster-relay-study
#13
Sara Elmahdi, Salina Parveen, Sylvia Ossai, Ligia V DaSilva, Michael Jahncke, John Bowers, John Jacobs
Vibrio parahaemolyticus (Vp) and Vibrio vulnificus (Vv) are naturally occurring estuarine bacteria and are the leading causes of seafood-associated infections and mortality in the USA. Though multiple-antibiotic resistant Vp and Vv have been reported resistance patterns in vibrios are not as well documented as other food-borne bacterial pathogens. Salinity relaying (SR) is a Post-Harvest Processing (PHP) treatment to reduce the abundance of these pathogens in shellfish harvested during the warmer months. The purpose of this study was to evaluate the antimicrobial susceptibility (AMS), pathogenicity and genetic profiles of Vp and Vv recovered from oysters during an oyster relay study...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29142160/immunogenicity-and-protective-efficacy-of-vibrio-vulnificus-flagellin-protein-flab-in-a-wound-infection-model
#14
Kohei Yamazaki, Takashige Kashimoto, Yuki Hashimoto, Takehiro Kado, Shunji Ueno
Vibrio vulnificus is known as an opportunistic bacterial pathogen that causes primary septicemia and wound infection in humans. Recently, the incidence of wound infection by V. vulnificus is increasing in warm countries. In this study, we examined a vaccine antigen against V. vulnificus in mice. FlaB, a component protein of the V. vulnificus flagellum, was expressed as a recombinant protein, named rFlaB. After immunization of mice with rFlaB, the mice were challenged by subcutaneous inoculation with V. vulnificus...
November 15, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29126967/effects-of-temperature-and-salinity-on-prevalence-and-intensity-of-infection-of-blue-crabs-callinectes-sapidus-by-vibrio-cholerae-v-parahaemolyticus-and-v-vulnificus-in-louisiana
#15
Timothy J Sullivan, Joseph E Neigel
Coastal marine and estuarine environments are experiencing higher average temperatures, greater frequency of extreme temperature events, and altered salinities. These changes are expected to stress organisms and increase their susceptibility to infectious diseases. However, beyond these generalities, little is known about how environmental factors influence host-pathogen relationships in the marine realm. We investigated the prevalence and intensity of infections by Vibrio cholerae, V. parahaemolyticus, and V...
November 7, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29115398/vibrio-vulnificus-infection-induces-the-maturation-and-activation-of-dendritic-cells-with-inflammatory-th17-polarizing-ability
#16
Arim Lee, Hui Xuan Lim, Myun Soo Kim, Daeho Cho, Kyung Ku Jang, Sang Ho Choi, Tae Sung Kim
Vibrio vulnificus (V. vulnificus) is a gram-negative bacterium, which causes life-threatening septicemia and gastroenteritis through the consumption of contaminated seafood or wound infection. In addition, V. vulnificus infection is known to stimulate the production of several pro-inflammatory cytokines, which are associated with inflammatory responses mediated predominantly by dendritic cells (DCs), functioning as antigen-presenting cells. The present study aimed to investigate whether V. vulnificus infection induced the maturation and activation of murine DCs, which have the ability to polarize T helper (Th) cells into Th17 cells...
November 1, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29112138/cytotoxic-and-antibacterial-compounds-from-the-coral-derived-fungus-aspergillus-tritici-sp2-8-1
#17
Weiyi Wang, Yanyan Liao, Chao Tang, Xiaomei Huang, Zhuhua Luo, Jianming Chen, Peng Cai
Three novel compounds, 4-methyl-candidusin A (1), aspetritone A (2) and aspetritone B (3), were obtained from the culture of a coral-derived fungus Aspergillus tritici SP2-8-1, together with fifteen known compounds (4-18). Their structures, including absolute configurations, were assigned based on NMR, MS, and time-dependent density functional theory (TD-DFT) ECD calculations. Compounds 2 and 5 exhibited better activities against methicillin-resistant strains of S. aureus (MRSA) ATCC 43300 and MRSA CGMCC 1...
November 7, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29054522/antimicrobial-activity-of-a-newly-identified-kazal-type-serine-proteinase-inhibitor-cckpi1-from-the-jellyfish-cyanea-capillata
#18
Yonghong Zhou, Guoyan Liu, Xi Cheng, Qianqian Wang, Bo Wang, Beilei Wang, Hui Zhang, Qian He, Liming Zhang
In this study, we reported a jellyfish-derived Kazal-type serine protease inhibitor, named CcKPI1, from Cyanea capillata. CcKPI1 has a calculated molecular mass of 19.02kDa and contains three typical Kazal domains. Soluble recombinant CcKPI1 (rCcKPI1) was successfully expressed and purified. rCcKPI1 exhibited significant inhibitory activities against elastase, subtilisin A and proteinase K, but not against trypsin or chymotrypsin. Kinetic studies showed that all of the inhibitory effects of rCcKPI1 were competitive, indicating that it may be a microbial serine protease inhibitor and can exhibit antimicrobial activity...
October 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29051620/antimicrobial-peptide-loaded-gold-nanoparticle-dna-aptamer-conjugates-as-highly-effective-antibacterial-therapeutics-against-vibrio-vulnificus
#19
Boeun Lee, Jonggwan Park, Minkyung Ryu, Soochan Kim, Minju Joo, Ji-Hyun Yeom, Suk Kim, Yoonkyung Park, Kangseok Lee, Jeehyeon Bae
Vibrio vulnificus causes fatal infections in humans, and antibiotics are commonly used in treatment regimens against V. vulnificus infection. However, the therapeutic effects of antibiotics are limited by multidrug resistance. In this study, we demonstrated that an antimicrobial peptide (AMP), HPA3P(His), loaded onto a gold nanoparticle-DNA aptamer (AuNP-Apt) conjugate (AuNP-Apt-HPA3P(His)) is an effective therapeutic tool against V. vulnificus infection in vivo in mice. HPA3P(His) induced bacterial cell death through the disruption of membrane integrity of V...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29045415/isolation-identification-and-phenotypic-and-molecular-characterization-of-pathogenic-vibrio-vulnificus-isolated-from-litopenaeus-vannamei
#20
Tao Teng, Liguo Liang, Kai Chen, Bingwen Xi, Jun Xie, Pao Xu
The morphology and the drug sensitivity of the strain GYX2014-1 isolated from the hepatic pancreatic tissue of moribund Litopenaeus vannamei were evaluated by conventional culture characteristics, physical and chemical characteristics, and molecular biology methods. Detection of extracellulase and hemolysin activity shows that the isolated GYX2014-1 has protease, lipase, gelatinase activity, but none of amylase, or lecithinase activity. The 16S rRNA gene (GenBank accession number: KT781675) was analyzed, and a phylogenetic tree analysis showed that the isolated pathogen was most closely related to V...
2017: PloS One
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