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https://www.readbyqxmd.com/read/28709640/genome-wide-identification-of-genes-essential-for%C3%A2-podocyte-cytoskeletons-based%C3%A2-on%C3%A2-single-cell-rna%C3%A2-sequencing
#1
Yuqiu Lu, Yuting Ye, Wenduona Bao, Qianqian Yang, Jinquan Wang, Zhihong Liu, Shaolin Shi
Gene expression differs substantially among individual cells of the same type. We speculate that genes that are expressed in all but a portion of cells of a given cell type would be likely essential and required for either the cell survival (housekeeping) or for the cell type's unique structure and function, enabling the organism to survive. Here, we performed RNA-seq of 20 mouse podocytes using the Fluidigm C1 system and identified 335 genes that were expressed in all of them. Among them, 239 genes were also expressed in mesangial and endothelial cells and were involved in energy metabolism, protein synthesis, etc...
November 2017: Kidney International
https://www.readbyqxmd.com/read/25222615/adaptive-human-cdkal1-variants-underlie-hormonal-response-variations-at-the-enteroinsular-axis
#2
Chia Lin Chang, James J Cai, Shang Yu Huang, Po Jen Cheng, Ho Yen Chueh, Sheau Yu Teddy Hsu
Recent analyses have identified positively selected loci that explain differences in immune responses, body forms, and adaptations to extreme climates, but variants that describe adaptations in energy-balance regulation remain underexplored. To identify variants that confer adaptations in energy-balance regulation, we explored the evolutionary history and functional associations of candidate variants in 207 genes. We screened single nucleotide polymorphisms in genes that had been associated with energy-balance regulation for unusual genetic patterns in human populations, followed by studying associations among selected variants and serum levels of GIP, insulin, and C-peptide in pregnant women after an oral glucose tolerance test...
2014: PloS One
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