Till Stephan, Christian Brüser, Markus Deckers, Anna M Steyer, Francisco Balzarotti, Mariam Barbot, Tiana S Behr, Gudrun Heim, Wolfgang Hübner, Peter Ilgen, Felix Lange, David Pacheu-Grau, Jasmin K Pape, Stefan Stoldt, Thomas Huser, Stefan W Hell, Wiebke Möbius, Peter Rehling, Dietmar Riedel, Stefan Jakobs
Mitochondrial function is critically dependent on the folding of the mitochondrial inner membrane into cristae; indeed, numerous human diseases are associated with aberrant crista morphologies. With the MICOS complex, OPA1 and the F1 Fo -ATP synthase, key players of cristae biogenesis have been identified, yet their interplay is poorly understood. Harnessing super-resolution light and 3D electron microscopy, we dissect the roles of these proteins in the formation of cristae in human mitochondria. We individually disrupted the genes of all seven MICOS subunits in human cells and re-expressed Mic10 or Mic60 in the respective knockout cell line...
July 15, 2020: EMBO Journal