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https://www.readbyqxmd.com/read/29320984/prevalence-and-antibiotic-susceptibility-of-uropathogens-from-cases-of-urinary-tract-infections-uti-in-shashemene-referral-hospital-ethiopia
#1
Wubalem Desta Seifu, Alemayehu Desalegn Gebissa
BACKGROUND: Urinary tract infection (UTI) remains to be one of the most common infectious diseases diagnosed in developing countries. And a widespread use of antibiotics against uropathogens has led to the emergence of antibiotic resistant species. A laboratory based cross-sectional survey was conducted in Shashemene referral hospital to determine the prevalence and antibiotic susceptibility of uropathogens. METHODS: We have collected 384 clean catch mid-stream urine samples from all suspected UTI outpatients using sterile screw capped container...
January 10, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29319528/metagenomic-profiling-of-the-bacterial-community-changes-from-koji-to-mash-stage-in-the-brewing-of-soy-sauce
#2
Hongbin Wang, Quanzeng Wei, Shuqi Gui, Yongrui Feng, Yong Zhang, Yihan Liu, Fuping Lu
The improvement of soy sauce fermentation is restricted by the insufficient information on bacterial community. In this study, bacterial communities in the koji and mash stage were compared based on next-generation sequencing technology. A total of 29 genera were identified in the koji stage, while 34 in the mash stage. After koji stage, 7 genera disappeared and 12 new genera appeared in the mash stage. The dominant bacteria were Kurthia, Weissella and Staphylococcus in the koji stage and Staphylococcus, Kurthia, Enterococcus and Leuconostoc in the mash stage...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29282402/acute-endophthalmitis-caused-by-leuconostoc-spp-following-intravitreal-bevacizumab-injection
#3
Supreet Singh, Chirag V Patel, Kamal Kishore
We present a case of acute endophthalmitis caused by Leuconostoc spp. following intravitreal bevacizumab injection. An 86-year-old immunocompetent female developed acute endophthalmitis after intravitreal injection of bevacizumab for neovascular age-related macular degeneration. The patient presented with pain, visual acuity of hand motions, hypopyon, and dense vitritis 96 h after treatment. She was treated with vitreous and anterior chamber tap followed by intravitreal injections of 1 mg vancomycin, 2.25 mg ceftazidime, and 400 μg dexamethasone...
September 2017: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/29273963/transglycosylation-of-gallic-acid-by-using-leuconostoc-glucansucrase-and-its-characterization-as-a-functional-cosmetic-agent
#4
Seung-Hee Nam, Jeongjin Park, Woojin Jun, Doman Kim, Jin-A Ko, A M Abd El-Aty, Jin Young Choi, Do-Ik Kim, Kwang-Yeol Yang
Gallic acid glycoside was enzymatically synthesized by using dextransucrase and sucrose from gallic acid. After purification by butanol partitioning and preparative HPLC, gallic acid glucoside was detected at m/z 355 (C13, H16, O10, Na)+ by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The yield of gallic acid glucoside was found to be 35.7% (114 mM) by response surface methodology using a reaction mixture of 319 mM gallic acid, 355 mM sucrose, and 930 mU/mL dextransucrase...
December 22, 2017: AMB Express
https://www.readbyqxmd.com/read/29247054/condensed-tannins-affect-bacterial-and-fungal-microbiomes-and-mycotoxin-production-during-ensiling-and-upon-aerobic-exposure
#5
Kai Peng, Long Jin, Yan D Niu, Qianqian Huang, Tim A McAllister, Hee Eun Yang, Hubert Denise, Zhongjun Xu, Surya Acharya, Shunxi Wang, Yuxi Wang
Purple prairie clover (PPC; Dalea purpurea Vent.) containing 84.5 g/kg DM of condensed tannin (CT) was ensiled without (Control) or with polyethylene glycol (PEG) for 76 days, followed by 14 days of aerobic exposure. Changes in fermentation characteristics were determined and bacterial and fungal communities were assessed using metagenomic sequencing. Addition of PEG that deactivated CT at ensiling increased (P < 0.05∼0.001) soluble N, non-protein N, lactic acid, total volatile fatty acids, ammonia N, deoxynivalenol (DON) and ochratoxin A (OTA), but decreased (P < 0...
December 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29240968/monitoring-of-spoilage-associated-microbiota-on-modified-atmosphere-packaged-beef-and-differentiation-of-psychrophilic-and-psychrotrophic-strains
#6
Maik Hilgarth, Jürgen Behr, Rudi F Vogel
AIMS: This study aimed to explore the discriminatory power of MALDI-TOF MS as a high-throughput method to monitor growth dynamics of meat-spoiling bacteria on modified atmosphere packaged (MAP) beef and to differentiate psychrophilic and psychrotrophic spoilage-associated strains. METHODS AND RESULTS: MAP beef steaks were incubated for 21 days at constant 4 and 10 °C. Development of headspace gas composition, pH, CFU and spoilage-associated microbial composition were monitored...
December 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29238459/isolation-and-characterization-of-probiotics-from-dairies
#7
Babak Haghshenas, Yousef Nami, Ali Almasi, Norhafizah Abdullah, Dayang Radiah, Rozita Rosli, Abolfazl Barzegari, Ahmad Yari Khosroushahi
Background and Objectives: Probiotics are live microorganisms, which show beneficial health effects on hosts once consumed in sufficient amounts. LAB group can be isolated and characterized from traditional dairy sources. This study aimed at isolating, identifying, and in vitro characterizing (low pH/high bile salt tolerance, antibacterial activity, and antibiotic susceptibility) LAB strains from traditional Iranian dairy products. Materials and Methods: Isolated strains were identified by Gram staining, catalase assay, and 3 molecular identification methods; namely, (GTG) 5-PCR fingerprinting, ARDRA, and 16S rDNA gene sequencing...
August 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29222100/diversity-of-lactococcus-lactis-in-undefined-mixed-dairy-starter-cultures-revealed-by-comparative-genome-analyses-and-targeted-amplicon-sequencing-of-epsd
#8
Cyril A Frantzen, Hans Petter Kleppen, Helge Holo
Undefined mesophilic mixed (DL) starter cultures are used in the production of continental cheeses and contain unknown strain mixtures of Lactococcus lactis and leuconostocs. The choice of starter culture affects the taste, aroma and quality of the final product. To gain insight into the diversity of Lactococcus lactis strains in starter cultures, we whole-genome sequenced 95 isolates from three different starter cultures. Pan-genomic analyses, which included 30 publically available complete genomes, grouped the strains into 21 subsp...
December 8, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29196124/combined-engineering-of-disaccharide-transport-and-phosphorolysis-for-enhanced-atp-yield-from-sucrose-fermentation-in-saccharomyces-cerevisiae
#9
Wesley Leoricy Marques, Robert Mans, Ryan K Henderson, Eko Roy Marella, Jolanda Ter Horst, Erik de Hulster, Bert Poolman, Jean-Marc Daran, Jack T Pronk, Andreas K Gombert, Antonius J A van Maris
Anaerobic industrial fermentation processes do not require aeration and intensive mixing and the accompanying cost savings are beneficial for production of chemicals and fuels. However, the free-energy conservation of fermentative pathways is often insufficient for the production and export of the desired compounds and/or for cellular growth and maintenance. To increase free-energy conservation during fermentation of the industrially relevant disaccharide sucrose by Saccharomyces cerevisiae, we first replaced the native yeast α-glucosidases by an intracellular sucrose phosphorylase from Leuconostoc mesenteroides (LmSPase)...
November 28, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/29192074/draft-genome-sequence-of-leuconostoc-citreum-cw28-isolated-from-pozol-a-pre-hispanic-fermented-corn-beverage
#10
Clarita Olvera, Rosa I Santamaría, Patricia Bustos, Cristina Vallejo, Juan J Montor, Carmen Wacher, Agustín Lopez Munguia
Leuconostoc citreum CW28 was isolated from pozol, a Mayan fermented corn beverage. This strain produces a cell-associated inulosucrase, the first described in bacteria. Its draft genome sequence, announced here, has an estimated size of 1.98 Mb and harbors 1,915 coding genes, 12 rRNAs, 68 tRNAs, 17 putative pseudogenes, and 1 putative phage.
November 30, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29178882/characterization-and-susceptibility-of-streptococci-and-enterococci-isolated-from-nile-tilapia-oreochromis-niloticus-showing-septicaemia-in-aquaculture-and-wild-sites-in-egypt
#11
Kamelia M Osman, Khalid S Al-Maary, Aymen S Mubarak, Turki M Dawoud, Ihab M I Moussa, Mai D S Ibrahim, Ashgan M Hessain, Ahmed Orabi, Nehal M Fawzy
BACKGROUND: The present investigation was an endeavor into the elucidation of the disease-causing pathogen of streptococcosis in Nile tilapia (Oreochromis niloticus) in Egypt affecting adult fish cultured and wild fish in the Nile river. Fish were obtained from commercial fishermen, collected as part of their routine fishing activities. The researchers observed the routine fishing process and selected fish for use in the study, at the point of purchase from the fisherman. RESULTS: Diseased fish showed exophthalmia with accumulation of purulent and haemorrhagic fluid around eyes, and ventral petechial haemorrhages...
November 25, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29177756/leucocin-c-607-a-novel-bacteriocin-from-the-multiple-bacteriocin-producing-leuconostoc-pseudomesenteroides-607-isolated-from-persimmon
#12
Yi-Sheng Chen, Hui-Chung Wu, Cheng-Yu Kuo, Yu-Wei Chen, Sin Ho, Fujitoshi Yanagida
Leuconostoc pseudomesenteroides 607, isolated from persimmon fruit, was found to have high inhibitory activity against Listeria monocytogenes and several other Gram-positive bacteria. Inhibitory substances were purified from culture supernatant by ion-exchange chromatography, Sep-Pak C18 cartridge, and reverse-phase high-performance liquid chromatography (RP-HPLC). Two antibacterial peptides were observed during the purification procedures. One of these peptides had a molecular size of 4623.05 Da and a partial N-terminal amino acid sequence of NH2-KNYGNGVHxTKKGxS, in which the YGNGV motif is specific for class IIa bacteriocins...
November 25, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29175763/novel-insights-into-microbial-community-dynamics-during-the-fermentation-of-central-european-ice-wine
#13
Mária Bučková, Andrea Puškárová, Katarína Ženišová, Lucia Kraková, Ľubica Piknová, Tomáš Kuchta, Domenico Pangallo
Culture-dependent and culture-independent strategies were applied to investigate the microbiota of autumn undamaged and damaged berries, winter berries and ice wine must samples of Grüner Veltliner (Veltlínske zelené) from Small Carpathian wine-producing region. One hundred twenty-six yeasts and 242 bacterial strains isolated from several microbiological media (YPD, PDA, R2A, GYC, MRS and MRS-T) were clustered by ITS-PCR and subsequent Qiaxcel electrophoresis. Representatives of each cluster were identified by sequencing...
November 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29173621/polyphasic-approach-to-study-physico-chemical-microbiological-and-sensorial-characteristics-of-artisanal-nicastrese-goat-s-cheese
#14
Alessandra Pino, Luigi Liotta, Cinzia L Randazzo, Aldo Todaro, Agata Mazzaglia, Floro De Nardo, Vincenzo Chiofalo, Cinzia Caggia
Nicastrese goat's cheese is produced in the South of Italy under traditional procedures, from raw goat milk without any starter cultures addition. Samples from milk to ripened cheese provided by 4 different farms were subjected to a polyphasic approach to study their physico-chemical, microbiological and sensorial characteristics. In addition, volatile organic compounds formation in the final products was studied. Overall, gross composition and microbiological data revealed a significant variability among samples, which was confirmed by both the volatile organic compounds generated in the final products and by the sensorial data...
April 2018: Food Microbiology
https://www.readbyqxmd.com/read/29166640/lactic-acid-bacteria-from-andean-grain-amaranth-a-source-of-vitamins-and-functional-value-enzymes
#15
Silvana L Carrizo, Cecilia E Montes de Oca, María Elvira Hébert, Lucila Saavedra, Graciela Vignolo, Jean Guy LeBlanc, Graciela Celestina Rollán
Amaranth is a rediscovered pseudocereal with high nutritional properties. Lactic acid fermentation can increase the functional and nutritional value of foods. The aims of this study were to isolate and evaluate the functionality of lactic acid bacteria (LAB) from amaranth. LAB strains (n = 29) isolated from amaranth sourdough and grains included Lactobacillus (L.) plantarum (n = 8), L. rhamnosus (n = 6), Enterococcus (E.) mundtii (n = 4), E. hermanniensis (n = 3), E. durans (n = 1), Enterococcus sp. (n = 1), Leuconostoc (Lc...
November 23, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29147021/genome-scale-modeling-and-transcriptome-analysis-of-leuconostoc-mesenteroides-unravel-the-redox-governed-metabolic-states-in-obligate-heterofermentative-lactic-acid-bacteria
#16
Lokanand Koduru, Yujin Kim, Jeongsu Bang, Meiyappan Lakshmanan, Nam Soo Han, Dong-Yup Lee
Obligate heterofermentative lactic acid bacteria (LAB) are well-known for their beneficial health effects in humans. To delineate the incompletely characterized metabolism that currently limits their exploitation, at systems-level, we developed a genome-scale metabolic model of the representative obligate heterofermenting LAB, Leuconostoc mesenteroides (iLME620). Constraint-based flux analysis was then used to simulate several qualitative and quantitative phenotypes of L. mesenteroides, thereby evaluating the model validity...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146249/composition-of-lactic-acid-bacteria-during-spontaneous-curly-kale-brassica-oleracea-var-sabellica-fermentation
#17
Magdalena Michalak, Klaudia Gustaw, Adam Waśko, Magdalena Polak-Berecka
The present work is the first report on spontaneous fermentation of curly kale and characteristics of autochthonous lactic acid bacteria (LAB). Our results indicate that curly kale fermentation is the new possibility of the technological use of this vegetable. Bacteria representing ten different species were isolated from three phases of curly kale fermentation and identified by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. Among them, four species were identified as Lactobacillus spp. (Lb. plantarum 332, Lb...
January 2018: Microbiological Research
https://www.readbyqxmd.com/read/29136413/first-metagenomic-survey-of-the-microbial-diversity-in-bioaerosols-emitted-in-waste-sorting-plants
#18
Jodelle Degois, Frederic Clerc, Xavier Simon, Cyril Bontemps, Pierre Leblond, Philippe Duquenne
Waste sorting activities are source of occupational bioaerosol exposures that are associated with several health disorders. New analytical tools, based on next-generation sequencing (NGS) technologies, provide powerful methods to assess the microbial composition of bioaerosols. The objectives of the study were (i) to assess the feasibility and the repeatability of NGS-based biodiversity measurements and (ii) to study the microbial biodiversity using NGS in bioaerosols emitted in a waste sorting plant (WSP)...
November 10, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/29129229/head-injury-profoundly-affects-gut-microbiota-homeostasis-results-of-a-pilot-study
#19
Anne-Judith Waligora-Dupriet, Sophie Lafleur, Christine Charrueau, Caroline Choisy, Luc Cynober, Marie-José Butel, Christophe Moinard
OBJECTIVES: Head injury (HI) induces a hypercatabolic state, dysimmunity, and septic complications that increase morbidity and mortality. Although compromised immune function is usually incriminated in infection occurrence, gut dysbiosis could also be involved in this phenomenon and, to our knowledge, has never been considered. To assess if HI could affect microbiota, we explored the impact of HI on intestinal microbiota in a rodent model of fluid percussion. METHODS: Nineteen rats were randomly assigned to two groups: Healthy rats fed ad libitum (n = 7) and HI rats (n = 12), which received standard enteral nutrition for 4 d...
January 2018: Nutrition
https://www.readbyqxmd.com/read/29101023/new-dextransucrase-purification-process-of-the-enzyme-produced-by-leuconostoc-mesenteroides-ibun-91-2-98-based-on-binding-product-and-dextranase-hydrolysis
#20
Glaehter Yhon Flórez Guzman, Gustavo Buitrago Hurtado, Sonia Amparo Ospina
This paper examines a new dextransucrase (DS) purification process of the extracellular enzyme (EC 2.4.1.5) produced by Leuconostoc mesenteroides IBUN 91.2.98. The enzyme was purified using a methodology which combines the immobilization of the enzyme in the produced biopolymer dextran, followed by a concentration step by ultrafiltration, using a membrane with a pore size of 300kDa and subsequent hydrolysis of dextran by action of a dextranase and finally enzyme purification by anion exchange chromatography...
October 31, 2017: Journal of Biotechnology
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