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Silvana L Carrizo, Cecilia E Montes de Oca, María Elvira Hébert, Lucila Saavedra, Graciela Vignolo, Jean Guy LeBlanc, Graciela Celestina Rollán
Amaranth is a rediscovered pseudocereal with high nutritional properties. Lactic acid fermentation can increase the functional and nutritional value of foods. The aims of this study were to isolate and evaluate the functionality of lactic acid bacteria (LAB) from amaranth. LAB strains (n = 29) isolated from amaranth sourdough and grains included Lactobacillus (L.) plantarum (n = 8), L. rhamnosus (n = 6), Enterococcus (E.) mundtii (n = 4), E. hermanniensis (n = 3), E. durans (n = 1), Enterococcus sp. (n = 1), Leuconostoc (Lc...
November 23, 2017: Journal of Molecular Microbiology and Biotechnology
Lokanand Koduru, Yujin Kim, Jeongsu Bang, Meiyappan Lakshmanan, Nam Soo Han, Dong-Yup Lee
Obligate heterofermentative lactic acid bacteria (LAB) are well-known for their beneficial health effects in humans. To delineate the incompletely characterized metabolism that currently limits their exploitation, at systems-level, we developed a genome-scale metabolic model of the representative obligate heterofermenting LAB, Leuconostoc mesenteroides (iLME620). Constraint-based flux analysis was then used to simulate several qualitative and quantitative phenotypes of L. mesenteroides, thereby evaluating the model validity...
November 16, 2017: Scientific Reports
Magdalena Michalak, Klaudia Gustaw, Adam Waśko, Magdalena Polak-Berecka
The present work is the first report on spontaneous fermentation of curly kale and characteristics of autochthonous lactic acid bacteria (LAB). Our results indicate that curly kale fermentation is the new possibility of the technological use of this vegetable. Bacteria representing ten different species were isolated from three phases of curly kale fermentation and identified by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. Among them, four species were identified as Lactobacillus spp. (Lb. plantarum 332, Lb...
January 2018: Microbiological Research
Jodelle Degois, Frederic Clerc, Xavier Simon, Cyril Bontemps, Pierre Leblond, Philippe Duquenne
Waste sorting activities are source of occupational bioaerosol exposures that are associated with several health disorders. New analytical tools, based on next-generation sequencing (NGS) technologies, provide powerful methods to assess the microbial composition of bioaerosols. The objectives of the study were (i) to assess the feasibility and the repeatability of NGS-based biodiversity measurements and (ii) to study the microbial biodiversity using NGS in bioaerosols emitted in a waste sorting plant (WSP)...
November 10, 2017: Annals of Work Exposures and Health
Anne-Judith Waligora-Dupriet, Sophie Lafleur, Christine Charrueau, Caroline Choisy, Luc Cynober, Marie-José Butel, Christophe Moinard
OBJECTIVES: Head injury (HI) induces a hypercatabolic state, dysimmunity, and septic complications that increase morbidity and mortality. Although compromised immune function is usually incriminated in infection occurrence, gut dysbiosis could also be involved in this phenomenon and, to our knowledge, has never been considered. To assess if HI could affect microbiota, we explored the impact of HI on intestinal microbiota in a rodent model of fluid percussion. METHODS: Nineteen rats were randomly assigned to two groups: Healthy rats fed ad libitum (n = 7) and HI rats (n = 12), which received standard enteral nutrition for 4 d...
January 2018: Nutrition
Glaehter Yhon Flórez Guzman, Gustavo Buitrago Hurtado, Sonia Amparo Ospina
This paper examines a new dextransucrase (DS) purification process of the extracellular enzyme (EC produced by Leuconostoc mesenteroides IBUN 91.2.98. The enzyme was purified using a methodology which combines the immobilization of the enzyme in the produced biopolymer dextran, followed by a concentration step by ultrafiltration, using a membrane with a pore size of 300kDa and subsequent hydrolysis of dextran by action of a dextranase and finally enzyme purification by anion exchange chromatography...
October 31, 2017: Journal of Biotechnology
Lenny S F Ahmadsah, Eiseul Kim, Youn-Sik Jung, Hae-Yeong Kim
Laru samples obtained from the upper part of Borneo Island were analyzed for their lactic acid bacterial (LAB) and fungal diversity using both a culture-independent method (PCR-DGGE) and culture-dependent methods (SDS-PAGE and MALDI-TOF MS). Pediococcus pentosaceus, Lactobacillus brevis, Saccharomycopsis fibuligera, Hyphopichia burtonii, and Kodamaea ohmeri were detected by all three methods. In addition, Weissella cibaria, Weissella paramesenteroides, Leuconostoc citreum, Leuconostoc mesenteroides, Lactococcus lactis, Rhizopus oryzae/Amylomyces rouxii, Mucor indicus, and Candida intermedia were detected by PCR-DGGE...
October 30, 2017: Journal of Microbiology and Biotechnology
Yousra Abid, Angela Casillo, Houda Gharsallah, Ichrak Joulak, Rosa Lanzetta, Maria Michela Corsaro, Hamadi Attia, Samia Azabou
In this work, four exopolysaccharide-producing lactic acid bacteria (LAB) strains, newly isolated from Tunisian spontaneously fermented foods and beverages, namely bovine and turkey meat sausages (BMS and TMS), date palm sap (DPS) and cow milk (CM), were identified as Leuconostoc citreum-BMS, Leuconostoc mesenteroides-TMS, Pediococcus pentosaceus-DPS and Leuconostoc pseudomesenteroides-CM, respectively. The isolated strains showed the ability to withstand simulated human gastrointestinal (GI) tract conditions (low pH, lysozyme, bile salts, pepsin and pancreatin) and showed high surface hydrophobicity (79-90%), besides their ability to act against Escherichia coli and Listeria monocytogenes and to produce exopolysaccharides (EPS)...
October 25, 2017: International Journal of Biological Macromolecules
Maiia M Ananieva, Mariia O Faustova, Iaroslav O Basarab, Galina A Loban'
INTRODUCTION: Recently, opportunistic microflora are increasingly known to be involved in the development of pathological processes in various systems and organs. This situation promotes interest in their detailed study as causative agents of bacterial infections. THE AIM: To study the microbial species residing in carious cavities in acute profound caries. MATERIALS AND METHODS: The study involved 14 people with a diagnosis of acute profound caries...
2017: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
Alba Pérez-Cataluña, Patricia Elizaquível, Purificación Carrasco, Judith Espinosa, Dolores Reyes, Carmen Wacher, Rosa Aznar
The purpose of this work was to analyse the diversity and dynamics of lactic acid bacteria (LAB) throughout the fermentation process in Atole agrio, a traditional maize based food of Mexican origin. Samples of different fermentation times were analysed using culture-dependent and -independent approaches. Identification of LAB isolates revealed the presence of members of the genera Pediococcus, Weissella, Lactobacillus, Leuconostoc and Lactococcus, and the predominance of Pediococcus pentosaceus and Weissella confusa in liquid and solid batches, respectively...
October 22, 2017: Antonie Van Leeuwenhoek
Mariem Souissi, Rached Laabidi, Patricia Aissa, Olivier Pringault, Olfa Ben Said
The waste water treatment plant (WWTP) of the city of Bizerte concentrates different types of chemical and biological pollutants in the Bizerte lagoon (Tunisia). Considering four upstream and downstream WWTP discharge stations, seventy nine, culturable bacterial strains were isolated and identified from water and sediment as fecal coliforms, fecal streptococci, pathogenic staphylococci and non-enterobacteriacea. Fecal coliforms were most abundant (2.5 10(5) bacteria/mg) in sediment of WWTP discharge. Leuconostoc spp (23...
October 18, 2017: Ecotoxicology and Environmental Safety
Qingqing Zhou, Fang Feng, Yanfang Yang, Fangkun Zhao, Renpeng Du, Zhijiang Zhou, Ye Han
A water-souble exopolysaccharide (EPS) produced by Leuconostoc pseudomesenteroides XG5 from homemade wine was investigated. The EPS yield of 35.5g/L was achieved at 30°C for 48h in De Man-Rogosa-Sharpe (MRS) medium containing 12.5% sucrose. The EPS was a dextran composed exclusively of glucose and the molecular weight was 2.6×10(6)Da. Fourier transform infrared spectra and nuclear magnetic resonance spectra revealed that the EPS was a dextran containing D-glucose residues in a linear chain with consecutive α-(1→6) linkages...
October 16, 2017: International Journal of Biological Macromolecules
Soo-Young Choi, Seung-Hyun Ryu, Jin-Il Park, Eui-Suk Jeong, Jong-Hyung Park, Seung-Hoon Ham, Hee-Yeon Jeon, Jun-Young Kim, Inn-Goo Kyeong, Dong-Goo Kim, Ji-Young Shin, Yang-Kyu Choi
Robusta beans cultivated with Monascus ruber (RMR) were successively fermented with Leuconostoc mesenteroides (LM) and the antiobesity effects were examined. To produce an obese mouse model to investigate the hypolipidemic effects, ICR mice were fed the same high-fat diet for 6 weeks. Treatment groups were given 10 or 20% RMR-LM. Body weight changes in the 20% RMR-LM group were lower compared with those in the control group. Visceral adipose tissue weight and adipose size were significantly lower in the 20% RMR-LM group compared with those in the control group...
October 2017: Experimental and Therapeutic Medicine
Cristian Botta, Ilario Ferrocino, Maria Chiara Cavallero, Simonetta Riva, Manuela Giordano, Luca Cocolin
The microbial contamination that occurs during the slaughtering process and during handling of the meat results in a shortening of the shelf-life of meat. In this study, which has had the aim of extending the shelf life of beefsteaks, pilot-scale treatments were carried out with aqueous ozone (AO) and electrolyzed water (EW) before vacuum packaging (VP). The development of the potentially active microbiota and the associated volatilome were followed over 15days of storage under refrigerated conditions (4°C), in order to define the potential long-term effects of the treatments and storage condition on microbiota...
October 7, 2017: International Journal of Food Microbiology
Eun Ji Jeong, Dae Won Moon, Joon Suk Oh, Jin Seok Moon, Hyunbin Seong, Kwang Yup Kim, Nam Soo Han
Leuconostoc mesenteroides is used as a starter to produce high quality kimchi product. In this study, an efficient and economical cabbage juice medium (CJM) was developed by process optimization of cabbage extraction and pasteurization and by compositional supplementation of various lacking nutrients. The pasteurized cabbage juice was determined to be a good medium candidate to cultivate L. mesenteroides showing maximal cell numbers (9.85 × 10⁸ CFU/ml) after 24h. Addition of sucrose and yeast extract with soy peptone resulted in increment of bacterial cell counts in CJM showing supplementing effect of lacking nutrients...
October 14, 2017: Journal of Microbiology and Biotechnology
Meng-Qi Li, Hong-Bin Zhang, Yao Li, Xue-Qin Hu, Jing-Wen Yang
Dextransucrase (DSR, EC2.4.5.1) from Leuconostoc mesenteroides 0326 which catalyzes sucrose to produce dextran, are popularly used in food and medicine industries. However, its poor kinetic stability especially at higher temperatures is a limiting factor for developing industrial applications. The aim of this study is to improve the enzyme activity and thermal stability of dextransucrase by single, double and triple mutations of proline and lysine. In this work, Pro-473, Pro-678, and Pro-856 were selected as engineering targets and individually replaced with serine...
October 9, 2017: International Journal of Biological Macromolecules
Boyeon Park, Hyelyeon Hwang, Jina Lee, Sung-Oh Sohn, Se Hee Lee, Min Young Jung, Hyeong In Lim, Hae Woong Park, Jong-Hee Lee
BACKGROUND: Panax ginseng is a physiologically active plant widely used in traditional medicine that is characterized by the presence of ginsenosides. Rb1, a major ginsenoside, is used as the starting material for producing ginsenoside derivatives with enhanced pharmaceutical potentials through chemical, enzymatic, or microbial transformation. METHODS: To investigate the bioconversion of ginsenoside Rb1, we prepared kimchi originated bacterial strains Leuconostoc mensenteroides WiKim19, Pediococcus pentosaceus WiKim20, Lactobacillus brevis WiKim47, Leuconostoc lactis WiKim48, and Lactobacillus sakei WiKim49 and analyzed bioconversion products using LC-MS/MS mass spectrometer...
October 2017: Journal of Ginseng Research
M M Soltan Dallal, S Zamaniahari, A Davoodabadi, M Hosseini, Z Rajabi
Background: The pickle, a traditional fermented product, is popular among Iranians. Much research has been conducted worldwide on this food group. Due to a lack of related data in Iran, this study was conducted to isolate and identify dominant lactic acid bacteria (LAB) in pickles and salted pickles. Materials and methods: Seventy samples were collected from different regions of Iran. The isolated bacteria were identified as LAB by Gram staining and catalase by using MRS agar. Then, those strains were identified at the species level by physiological tests (e...
2017: GMS Hygiene and Infection Control
Lucas Tadeu Fuess, Antônio Djalma Nunes Ferraz, Carla Botelho Machado, Marcelo Zaiat
This study aimed to better understand the role of different microbial groups and the determining fermentation pathways in a biohydrogen (bioH2)-producing reactor fed with sugarcane vinasse by using next-generation sequencing and principal component analysis (PCA). Both microbial structure and dynamics were characterized. The highest bioH2 production levels were associated with the Thermoanaerobacterium genus, whilst lactate-producing bacteria comprised the dominant genera (e.g. Lactobacillus and Leuconostoc) within the reactor at both stable and unstable bioH2-producing periods...
September 21, 2017: Bioresource Technology
Ebrahim Elkhtab, Mohamed El-Alfy, Mohamed Shenana, Abdelaty Mohamed, Ahmed E Yousef
Compounds with the ability to inhibit angiotensin-converting enzyme (ACE) are used medically to treat human hypertension. The presence of such compounds naturally in food is potentially useful for treating the disease state. The goal of this study was to screen lactic acid bacteria, including species commonly used as dairy starter cultures, for the ability to produce new potent ACE-inhibiting peptides during milk fermentation. Strains of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus delbrueckii ssp...
September 27, 2017: Journal of Dairy Science
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