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https://www.readbyqxmd.com/read/29374777/single-cell-analysis-of-crohn-s-disease-patient-derived-small-intestinal-organoids-reveals-disease-activity-dependent-modification-of-stem-cell-properties
#1
Kohei Suzuki, Tatsuro Murano, Hiromichi Shimizu, Go Ito, Toru Nakata, Satoru Fujii, Fumiaki Ishibashi, Ami Kawamoto, Sho Anzai, Reiko Kuno, Konomi Kuwabara, Junichi Takahashi, Minami Hama, Sayaka Nagata, Yui Hiraguri, Kento Takenaka, Shiro Yui, Kiichiro Tsuchiya, Tetsuya Nakamura, Kazuo Ohtsuka, Mamoru Watanabe, Ryuichi Okamoto
BACKGROUND: Intestinal stem cells (ISCs) play indispensable roles in the maintenance of homeostasis, and also in the regeneration of the damaged intestinal epithelia. However, whether the inflammatory environment of Crohn's disease (CD) affects properties of resident small intestinal stem cells remain uncertain. METHODS: CD patient-derived small intestinal organoids were established from enteroscopic biopsy specimens taken from active lesions (aCD-SIO), or from mucosa under remission (rCD-SIO)...
January 27, 2018: Journal of Gastroenterology
https://www.readbyqxmd.com/read/29366750/defining-the-molecular-signatures-of-human-right-heart-failure
#2
Jordan L Williams, Omer Cavus, Emefah C Loccoh, Sara Adelman, John C Daugherty, Sakima A Smith, Benjamin Canan, Paul M L Janssen, Sara Koenig, Crystal F Kline, Peter J Mohler, Elisa A Bradley
AIMS: Right ventricular failure (RVF) varies significantly from the more common left ventricular failure (LVF). This study was undertaken to determine potential molecular pathways that are important in human right ventricular (RV) function and may mediate RVF. MATERIALS AND METHODS: We analyzed mRNA of human non-failing LV and RV samples and RVF samples from patients with pulmonary arterial hypertension (PAH), and post-LVAD implantation. We then performed transcript analysis to determine differential expression of genes in the human heart samples...
January 20, 2018: Life Sciences
https://www.readbyqxmd.com/read/29233684/mmu-mir-1963-negatively-regulates-the-ameloblast-differentiation-of-ls8-cell-line-by-directly-targeting-smoc2-3-utr
#3
Yue Wang, Huaiguang Chang, Haochen Liu, Yang Liu, Dong Han, Jinhao Xing, Hongshan Zhao, Hailan Feng
RUNX2 is a key regulator of osteogenic differentiation and odontoblastic differentiation. RUNX2 mutations could cause Cleidocranial dysplasia (CCD; OMIM119600), which is featured by abnormal development of bone and teeth. By using microRNA array, we identified a large number of microRNAs that showed different expression between wild-type Runx2 group and mutant groups. The aim of this study is to find out the effect of mmu-miR-1963, which was downregulated in all mutant Runx2 groups, on the ameloblast differentiation of LS8 cells...
December 9, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29151969/overexpression-of-smoc2-attenuates-the-tumorigenicity-of-hepatocellular-carcinoma-cells-and-is-associated-with-a-positive-postoperative-prognosis-in-human-hepatocellular-carcinoma
#4
Xu-Qiong Huang, Zi-Qi Zhou, Xiao-Fei Zhang, Chang-Long Chen, Yan Tang, Qian Zhu, Jian-Hua Zhang, Jian-Chuan Xia
Secreted modular calcium binding protein-2 (SMOC2), a recently identified matricellular protein that belongs to the SPARC protein family, has been reported to be downregulated in various cancers. The purpose of this study was to investigate the clinical significance and biological function of SMOC2 in human hepatocellular carcinoma. Real-time quantitative PCR and western blotting analyses revealed that SMOC2 mRNA and protein levels were significantly downregulated in human HCC tissues compared to the matched adjacent normal tissues...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28765789/deep-sequencing-reveals-variations-in-somatic-cell-mosaic-mutations-between-monozygotic-twins-with-discordant-psychiatric-disease
#5
Yoshiro Morimoto, Shinji Ono, Akira Imamura, Yuji Okazaki, Akira Kinoshita, Hiroyuki Mishima, Hideyuki Nakane, Hiroki Ozawa, Koh-Ichiro Yoshiura, Naohiro Kurotaki
Monozygotic (MZ) twins have been thought to be genetically identical. However, recent studies have shown discordant variants between them. We performed whole-exome sequencing (WES) in five MZ twin pairs with discordant neurodevelopmental disorders and one healthy control MZ twin to detect discordant variants. We identified three discordant variants confirmed by deep sequencing after analysis by personalized next-generation sequencing (NGS). Three mutations in FBXO38 (chr5:147774428;T>G), SMOC2 (chr6:169051385;A>G) and TDRP (chr8:442616;A>G), were detected with low allele frequency of mutant alleles on deep sequencing, suggesting that these loci are mosaic due to somatic mutations in a developmental stage...
2017: Human Genome Variation
https://www.readbyqxmd.com/read/28678915/increased-expression-of-id2-prelp-and-smoc2-genes-in-patients-with-endometriosis
#6
F M Araujo, J Meola, J C Rosa-E-Silva, C C P Paz, R A Ferriani, A A Nogueira
Endometriosis is a benign, estrogen-dependent disease with symptoms such as pelvic pain and infertility, and it is characterized by the ectopic distribution of endometrial tissue. The expression of the ID2, PRELP and SMOC2 genes was compared between the endometrium of women without endometriosis in the proliferative phase of their menstrual cycle and the eutopic and ectopic endometrium of women with endometriosis in the proliferative phase. Paired tissue samples from 20 women were analyzed: 10 from endometrial and peritoneal endometriotic lesions and 10 from endometrial and ovarian endometriotic lesions...
July 3, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28575239/whole-genome-cardiac-dna-methylation-fingerprint-and-gene-expression-analysis-provide-new-insights-in-the-pathogenesis-of-chronic-chagas-disease-cardiomyopathy
#7
Laurie Laugier, Amanda Farage Frade, Frederico Moraes Ferreira, Monique Andrade Baron, Priscila Camillo Teixeira, Sandrine Cabantous, Ludmila Rodrigues Pinto Ferreira, Laurence Louis, Vagner Oliveira Carvalho Rigaud, Fabio Antônio Gaiotto, Fernando Bacal, Pablo Pomerantzeff, Edimar Bocchi, Jorge Kalil, Ronaldo Honorato Barros Santos, Edecio Cunha-Neto, Christophe Chevillard
Background: Chagas disease, caused by the protozoan Trypanosoma cruzi, is endemic in Latin America and affects 10 million people worldwide. Approximately 12,000 deaths attributable to Chagas disease occur annually due to Chagas disease cardiomyopathy (CCC), an inflammatory cardiomyopathy presenting with heart failure and arrythmia; 30% of infected subjects develop CCC years after infection. Genetic mechanisms play a role in differential progression to CCC, but little is known about the role of epigenetic modifications in pathological gene expression patterns in CCC patients' myocardium...
May 30, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28552356/canine-brachycephaly-is-associated-with-a-retrotransposon-mediated-missplicing-of-smoc2
#8
Thomas W Marchant, Edward J Johnson, Lynn McTeir, Craig I Johnson, Adam Gow, Tiziana Liuti, Dana Kuehn, Karen Svenson, Mairead L Bermingham, Michaela Drögemüller, Marc Nussbaumer, Megan G Davey, David J Argyle, Roger M Powell, Sérgio Guilherme, Johann Lang, Gert Ter Haar, Tosso Leeb, Tobias Schwarz, Richard J Mellanby, Dylan N Clements, Jeffrey J Schoenebeck
In morphological terms, "form" is used to describe an object's shape and size. In dogs, facial form is stunningly diverse. Facial retrusion, the proximodistal shortening of the snout and widening of the hard palate is common to brachycephalic dogs and is a welfare concern, as the incidence of respiratory distress and ocular trauma observed in this class of dogs is highly correlated with their skull form. Progress to identify the molecular underpinnings of facial retrusion is limited to association of a missense mutation in BMP3 among small brachycephalic dogs...
June 5, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28422762/silencing-smoc2-ameliorates-kidney-fibrosis-by-inhibiting-fibroblast-to-myofibroblast-transformation
#9
Casimiro Gerarduzzi, Ramya K Kumar, Priyanka Trivedi, Amrendra K Ajay, Ashwin Iyer, Sarah Boswell, John N Hutchinson, Sushrut S Waikar, Vishal S Vaidya
Secreted modular calcium-binding protein 2 (SMOC2) belongs to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins whose members are known to modulate cell-matrix interactions. We report that SMOC2 is upregulated in the kidney tubular epithelial cells of mice and humans following fibrosis. Using genetically manipulated mice with SMOC2 overexpression or knockdown, we show that SMOC2 is critically involved in the progression of kidney fibrosis. Mechanistically, we found that SMOC2 activates a fibroblast-to-myofibroblast transition (FMT) to stimulate stress fiber formation, proliferation, migration, and extracellular matrix production...
April 20, 2017: JCI Insight
https://www.readbyqxmd.com/read/28356709/the-sparc-related-modular-calcium-binding-protein-2-smoc2-gene-polymorphism-in-primary-glaucoma-a-case-control-study
#10
Najwa Al-Dabbagh, Hamoud Al-Shahrani, Nourah Al-Dohayan, Md Mustafa, Misbahul Arfin, Abdulrahman K Al-Asmari
Primary glaucomas are among the most common eye diseases that may potentially result in bilateral blindness. Both genetics and environmental factors are reported to be involved in the etiology of primary glaucomas. Secreted protein acidic and rich in cysteine (SPARC)-related modular calcium binding protein 2 (SMOC2) is a matricellular glycoprotein encoded by the SMOC2 gene and known to regulate the expression of extracellular matrix (ECM) proteins and matrix metalloproteinases (MMPs), which play an important role in the pathogenesis of primary glaucomas...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28018113/smoc2-potentiates-proliferation-of-hepatocellular-carcinoma-cells-via-promotion-of-cell-cycle-progression
#11
Jing-Ran Su, Jing-Hua Kuai, Yan-Qing Li
AIM: To determine the influence of Smoc2 on hepatocellular carcinoma (HCC) cell proliferation and to find a possible new therapeutic target for preventing HCC progression. METHODS: We detected expression of Smoc2 in HCC tissues and corresponding non-tumor liver (CNL) tissues using PCR, western blot, and immunohistochemistry methods. Subsequently, we down-regulated and up-regulated Smoc2 expression using siRNA and lentivirus transfection assay, respectively. Then, we identified the effect of Smoc2 on cell proliferation and cell cycle using CCK-8 and flow cytometry, respectively...
December 7, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27150038/an-arntl2-driven-secretome-enables-lung-adenocarcinoma-metastatic-self-sufficiency
#12
Jennifer J Brady, Chen-Hua Chuang, Peyton G Greenside, Zoë N Rogers, Christopher W Murray, Deborah R Caswell, Ursula Hartmann, Andrew J Connolly, E Alejandro Sweet-Cordero, Anshul Kundaje, Monte M Winslow
The ability of cancer cells to establish lethal metastatic lesions requires the survival and expansion of single cancer cells at distant sites. The factors controlling the clonal growth ability of individual cancer cells remain poorly understood. Here, we show that high expression of the transcription factor ARNTL2 predicts poor lung adenocarcinoma patient outcome. Arntl2 is required for metastatic ability in vivo and clonal growth in cell culture. Arntl2 drives metastatic self-sufficiency by orchestrating the expression of a complex pro-metastatic secretome...
May 9, 2016: Cancer Cell
https://www.readbyqxmd.com/read/27020968/comparative-gene-expression-analysis-of-the-coronal-pulp-and-apical-pulp-complex-in-human-immature-teeth
#13
Soo-Hyun Kim, Seunghye Kim, Yooseok Shin, Hyo-Seol Lee, Mijeong Jeon, Seong-Oh Kim, Sung-Won Cho, Nikita B Ruparel, Je Seon Song
INTRODUCTION: This study determined the gene expression profiles of the human coronal pulp (CP) and apical pulp complex (APC) with the aim of explaining differences in their functions. METHODS: Total RNA was isolated from the CP and APC, and gene expression was analyzed using complementary DNA microarray technology. Gene ontology analysis was used to classify the biological function. Quantitative reverse-transcription polymerase chain reaction and immunohistochemical staining were performed to verify microarray data...
May 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/26794603/novel-elements-of-the-chondrocyte-stress-response-identified-using-an-in-vitro-model-of-mouse-cartilage-degradation
#14
Richard Wilson, Suzanne B Golub, Lynn Rowley, Constanza Angelucci, Yuliya V Karpievitch, John F Bateman, Amanda J Fosang
The destruction of articular cartilage in osteoarthritis involves chondrocyte dysfunction and imbalanced extracellular matrix (ECM) homeostasis. Pro-inflammatory cytokines such as interleukin-1α (IL-1α) contribute to osteoarthritis pathophysiology, but the effects of IL-1α on chondrocytes within their tissue microenvironment have not been fully evaluated. To redress this we used label-free quantitative proteomics to analyze the chondrocyte response to IL-1α within a native cartilage ECM. Mouse femoral heads were cultured with and without IL-1α, and both the tissue proteome and proteins released into the media were analyzed...
March 4, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/26613604/mensenchymal-stem-cells-can-delay-radiation-induced-crypt-death-impact-on-intestinal-cd44-fragments
#15
Peng-Yu Chang, Xing Jin, Yi-Yao Jiang, Li-Xian Wang, Yong-Jun Liu, Jin Wang
Intestinal stem cells are primitive cells found within the intestinal epithelium that play a central role in maintaining epithelial homeostasis through self-renewal and commitment into functional epithelial cells. Several markers are available to identify intestinal stem cells, such as Lgr5, CD24 and EphB2, which can be used to sort intestinal stem cells from mammalian gut. Here, we identify and isolate intestinal stem cells from C57BL/6 mice by using a cell surface antigen, CD44. In vitro, some CD44(+) crypt cells are capable of forming "villus-crypt"-like structures (organoids)...
May 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/26597826/genes-targeted-by-the-hedgehog-signaling-pathway-can-be-regulated-by-estrogen-related-receptor-%C3%AE
#16
Yuan Lu, Jilong Li, Jianlin Cheng, Dennis B Lubahn
BACKGROUND: Nuclear receptor family member, Estrogen related receptor β, and the Hedgehog signal transduction pathway are both reported to relate to tumorigenesis and induced pluripotent stem cell reprogramming. We hypothesize that Estrogen related receptor β can modulate the Hedgehog signaling pathway and affect Hedgehog driven downstream gene expression. RESULTS: We established an estrogen related receptor β-expressing Hedgehog-responsive NIH3T3 cell line by Esrrb transfection, and performed mRNA profiling using RNA-Seq after Hedgehog ligand conditioned medium treatment...
November 23, 2015: BMC Molecular Biology
https://www.readbyqxmd.com/read/26546417/unique-dna-methylation-patterns-in-offspring-of-hypertensive-pregnancy
#17
Colleen G Julian, Brent S Pedersen, Carlos Salinas Salmon, Ivana V Yang, Marcelino Gonzales, Enrique Vargas, Lorna G Moore, David A Schwartz
Epigenomic processes are believed to play a pivotal role for the effect of environmental exposures in early life to modify disease risk throughout the lifespan. Offspring of women with hypertensive complications of pregnancy (HTNPREG ) have an increased risk of developing systemic and pulmonary vascular dysfunction in adulthood. In this preliminary report, we sought to determine whether epigenetic modifications of genes involved in the regulation of vascular function were present in HTNPREG offspring. We contrasted DNA methylation and gene expression patterns of peripheral blood mononuclear cells obtained from young male offspring of HTNPREG (n = 5) to those of normotensive controls (n = 19)...
December 2015: Clinical and Translational Science
https://www.readbyqxmd.com/read/26359667/bioinformatics-based-identification-of-microrna-regulated-and-rheumatoid-arthritis-associated-genes
#18
Yi-Jiang Song, Guiling Li, Jian-Hua He, Yao Guo, Li Yang
MicroRNAs (miRNAs) act as epigenetic markers and regulate the expression of their target genes, including those characterized as regulators in autoimmune diseases. Rheumatoid arthritis (RA) is one of the most common autoimmune diseases. The potential roles of miRNA-regulated genes in RA pathogenesis have greatly aroused the interest of clinicians and researchers in recent years. In the current study, RA-related miRNAs records were obtained from PubMed through conditional literature retrieval. After analyzing the selected records, miRNA targeted genes were predicted...
2015: PloS One
https://www.readbyqxmd.com/read/26070663/unbalanced-translocations-arise-from-diverse-mutational-mechanisms-including-chromothripsis
#19
Brooke Weckselblatt, Karen E Hermetz, M Katharine Rudd
Unbalanced translocations are a relatively common type of copy number variation and a major contributor to neurodevelopmental disorders. We analyzed the breakpoints of 57 unique unbalanced translocations to investigate the mechanisms of how they form. Fifty-one are simple unbalanced translocations between two different chromosome ends, and six rearrangements have more than three breakpoints involving two to five chromosomes. Sequencing 37 breakpoint junctions revealed that simple translocations have between 0 and 4 base pairs (bp) of microhomology (n = 26), short inserted sequences (n = 8), or paralogous repeats (n = 3) at the junctions, indicating that translocations do not arise primarily from nonallelic homologous recombination but instead form most often via nonhomologous end joining or microhomology-mediated break-induced replication...
July 2015: Genome Research
https://www.readbyqxmd.com/read/25889587/influence-of-vascular-endothelial-growth-factor-stimulation-and-serum-deprivation-on-gene-activation-patterns-of-human-adipose-tissue-derived-stromal-cells
#20
Josefine Tratwal, Anders Bruun Mathiasen, Morten Juhl, Sonja Kim Brorsen, Jens Kastrup, Annette Ekblond
INTRODUCTION: Stimulation of mesenchymal stromal cells and adipose tissue-derived stromal cells (ASCs) with vascular endothelial growth factor (VEGF) has been used in multiple animal studies and clinical trials for regenerative purposes. VEGF stimulation is believed to promote angiogenesis and VEGF stimulation is usually performed under serum deprivation. Potential regenerative molecular mechanisms are numerous and the role of contributing factors is uncertain. The aim of the current study was to investigate the effect of in vitro serum deprivation and VEGF stimulation on gene expression patterns of ASCs...
April 13, 2015: Stem Cell Research & Therapy
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