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https://www.readbyqxmd.com/read/27914989/transcriptome-analysis-of-adult-and-fetal-trabecular-meshwork-cornea-and-ciliary-body-tissues-by-rna-sequencing
#1
Megan Ulmer Carnes, R Rand Allingham, Allison Ashley-Koch, Michael A Hauser
PURPOSE: To characterize the transcriptional landscape of human adult and fetal trabecular meshwork (TM), cornea, and ciliary body (CB) tissues, and to evaluate the expression level of candidate genes selected from genetic association studies of primary-open angle glaucoma, central corneal thickness, intraocular pressure, vertical cup to disc ratio, and optic nerve parameters. METHODS: Deep RNA sequencing was performed on the selected human tissues. Transcriptome analyses were performed to 1) characterize the total number of expressed genes, 2) identify the most highly expressed genes, 3) estimate the number of novel transcripts, and 4) evaluate the expression of candidate genes in each tissue...
November 30, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27909812/in-phyllodes-tumors-of-the-breast-expression-of-sparc-osteonectin-bm40-mrna-by-in-situ-hybridization-correlates-with-protein-expression-by-immunohistochemistry-and-is-associated-with-tumor-progression
#2
Nah Ihm Kim, Ga-Eon Kim, Ji Shin Lee, Min Ho Park
Secreted protein acidic and rich in cysteine (SPARC) plays an essential role in tumor invasion and metastasis. The present work was undertaken to detect expression of SPARC mRNA in phyllodes tumors (PTs) and its association with SPARC protein expression. This study also evaluated expression of SPARC mRNA and its correlation between grade and clinical behavior of PTs. In addition, we assessed in PTs the association of expression of SPARC with that of matrix metalloproteinase (MMP)-2 and of MMP-9. SPARC mRNA expression was determined by RNAscope in situ hybridization (ISH) in 50 benign, 22 borderline, and 10 malignant PTs using a tissue microarray...
December 1, 2016: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/27908613/sparc-interacts-with-actin-in-skeletal-muscle-in%C3%A2-vitro-and-in%C3%A2-vivo
#3
Louise H Jørgensen, Pia Lørup Jepsen, Anders Boysen, Line B Dalgaard, Lars G Hvid, Niels Ørtenblad, Dea Ravn, Jeeva Sellathurai, Jakob Møller-Jensen, Hanns Lochmüller, Henrik D Schrøder
The cytoskeleton is an integral part of skeletal muscle structure, and reorganization of the cytoskeleton occurs during various modes of remodeling. We previously found that the extracellular matrix protein secreted protein acidic and rich in cysteine (SPARC) is up-regulated and expressed intracellularly in developing muscle, during regeneration and in myopathies, which together suggests that SPARC might serve a specific role within muscle cells. Using co-immunoprecipitation combined with mass spectrometry and verified by staining for direct protein-protein interaction, we find that SPARC binds to actin...
November 28, 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27891773/cigarette-smoke-alters-the-secretome-of-lung-epithelial-cells
#4
Alessandra Mossina, Christina Lukas, Juliane Merl-Pham, Franziska E Uhl, Kathrin Mutze, Andrea Schamberger, Claudia Staab-Weijnitz, Jie Jia, Ali Ö Yildirim, Melanie Königshoff, Stefanie M Hauck, Oliver Eickelberg, Silke Meiners
Cigarette smoke is the most relevant risk factor for the development of lung cancer and chronic obstructive pulmonary disease (COPD). Many of its more than 4500 chemicals are highly reactive thereby altering protein structure and function. Here, we used subcellular fractionation coupled to label-free quantitative mass spectrometry to globally assess alterations in the proteome of different compartments of lung epithelial cells upon exposure to cigarette smoke extract. Proteomic profiling of the human alveolar-derived cell line A549 revealed the most pronounced changes within the cellular secretome with preferential downregulation of proteins involved in wound healing and extracellular matrix (ECM) organization...
November 28, 2016: Proteomics
https://www.readbyqxmd.com/read/27886258/a-holistic-approach-to-dissecting-sparc-family-protein-complexity-reveals-fstl-1-as-an-inhibitor-of-pancreatic-cancer-cell-growth
#5
Katrina Viloria, Amanda Munasinghe, Sharan Asher, Roberto Bogyere, Lucy Jones, Natasha J Hill
SPARC is a matricellular protein that is involved in both pancreatic cancer and diabetes. It belongs to a wider family of proteins that share structural and functional similarities. Relatively little is known about this extended family, but evidence of regulatory interactions suggests the importance of a holistic approach to their study. We show that Hevin, SPOCKs, and SMOCs are strongly expressed within islets, ducts, and blood vessels, suggesting important roles for these proteins in the normal pancreas, while FSTL-1 expression is localised to the stromal compartment reminiscent of SPARC...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27881195/the-involvement-of-runx2-and-sparc-genes-in-the-bacterial-chondronecrosis-with-osteomyelitis-in-broilers
#6
E Paludo, A M G Ibelli, J O Peixoto, F C Tavernari, C A V Lima-Rosa, J R C Pandolfi, M C Ledur
Economic losses due to an increase of leg disorders in broilers have become a major concern of the poultry industry. Despite the efforts to reduce skeletal abnormalities in chickens, insufficient progress has been made. Bacterial chondronecrosis with osteomyelitis (BCO) is one of the main disorders that affect bone integrity in broilers. However, the genetic pathways and genes involved in most bone problems, including BCO, remains unclear. In this study, femoral samples from male broilers with 45 days of age affected or not with BCO were used to compare the relative expression with a reverse transcription real time PCR approach of 13 candidate genes: SPP1 (osteopontin), TNFRSF11B (osteoprotegerin), SPARC (osteonectin), CALB1 (calbidin 1), CALM (Calmodulin 2), IBSP (sialoprotein), COL1A2 (collagen, type I, α 2), BMP2 (bone morphogenetic protein 2), BMP3 (bone morphogenetic protein 3), RANKL (κ-B nuclear factor ligand), SMAD1 (SMAD family member 1), LEPR (leptin receptor) and RUNX2 (related transcription factor Runt 2)...
November 24, 2016: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/27880844/atorvastatin-improves-ventricular-remodeling-after-myocardial-infarction-by-interfering-with-collagen-metabolism
#7
Karla Reichert, Helison Rafael Pereira do Carmo, Anali Galluce Torina, Daniela Diógenes de Carvalho, Andrei Carvalho Sposito, Karlos Alexandre de Souza Vilarinho, Lindemberg da Mota Silveira-Filho, Pedro Paulo Martins de Oliveira, Orlando Petrucci
PURPOSE: Therapeutic strategies that modulate ventricular remodeling can be useful after acute myocardial infarction (MI). In particular, statins may exert effects on molecular pathways involved in collagen metabolism. The aim of this study was to determine whether treatment with atorvastatin for 4 weeks would lead to changes in collagen metabolism and ventricular remodeling in a rat model of MI. METHODS: Male Wistar rats were used in this study. MI was induced in rats by ligation of the left anterior descending coronary artery (LAD)...
2016: PloS One
https://www.readbyqxmd.com/read/27878658/role-of-matricellular-proteins-in-disorders-of-the-central-nervous-system
#8
A R Jayakumar, A Apeksha, M D Norenberg
Matricellular proteins (MCPs) are actively expressed non-structural proteins present in the extracellular matrix, which rapidly turnover and possess regulatory roles, as well as mediate cell-cell interactions. MCPs characteristically contain binding sites for other extracellular proteins, cell surface receptors, growth factors, cytokines and proteases, that provide structural support for surrounding cells. MCPs are present in most organs, including brain, and play a major role in cell-cell interactions and tissue repair...
November 23, 2016: Neurochemical Research
https://www.readbyqxmd.com/read/27873118/current-update-of-a-carboxymethylcellulose-peg-conjugate-platform-for-delivery-of-insoluble-cytotoxic-agents-to-tumors
#9
Yang Yang, Joseph Bteich, Shyh-Dar Li
Cytotoxic chemotherapeutic agents are used as the standard therapy for a range of significant cancers, but many of these drugs suffer from poor water solubility and low selectivity, limiting their clinical efficacy. To overcome these shortcomings, Cellax™ drug delivery platform was developed. Cellax™ is a polymer-based nanoparticle drug delivery system designed to solubilize hydrophobic drugs and target them to solid tumors, thereby enhancing the efficacy and reducing the side effects. Cellax-docetaxel (Cellax-DTX) displayed improved pharmacokinetic, safety, and efficacy profiles compared to native DTX (Taxotere®) and Nab-paclitaxel (Nab-PTX, Abraxane®) in multiple animal models...
November 21, 2016: AAPS Journal
https://www.readbyqxmd.com/read/27869083/spot-scanning-proton-arc-sparc-therapy-the-first-robust-and-delivery-efficient-spot-scanning-proton-arc-therapy
#10
Xuanfeng Ding, Xiaoqiang Li, J Michele Zhang, Peyman Kabolizadeh, Craig Stevens, Di Yan
PURPOSE: To present a novel robust and delivery-efficient spot-scanning proton arc (SPArc) therapy technique. METHODS AND MATERIALS: A SPArc optimization algorithm was developed that integrates control point resampling, energy layer redistribution, energy layer filtration, and energy layer resampling. The feasibility of such a technique was evaluated using sample patients: 1 patient with locally advanced head and neck oropharyngeal cancer with bilateral lymph node coverage, and 1 with a nonmobile lung cancer...
December 1, 2016: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/27856226/indications-utilization-and-complications-following-prostate-biopsy-a-new-york-state-analysis
#11
Joshua A Halpern, Art Sedrakyan, Brian Dinerman, Wei-Chun Hsu, Jialin Mao, Jim C Hu
PURPOSE: Uptake of active surveillance and changes in prostate cancer care may affect utilization of and complications following prostate needle biopsy (PNBx). We characterized recent trends and risk factors for PNBx complications using a statewide, all-payer cohort. MATERIALS AND METHODS: We utilized the New York Statewide Planning and Research Cooperation System (SPARCS) to identify PNBx performed between 2011 and 2014 via transrectal (N=9472) and transperineal (N=421) approach...
November 14, 2016: Journal of Urology
https://www.readbyqxmd.com/read/27845301/effects-of-epinephrine-on-angiogenesis-related-gene-expressions-in-cultured-rat-cardiomyocytes
#12
Henry Liu, Lisa Sangkum, Geoffrey Liu, Michael Green, Marilyn Li, Alan Kaye
Epinephrine is often used for the treatment of patients with heart failure, low cardiac output and cardiac arrest. It can acutely improve hemodynamic parameters; however, it does not seem to improve longer term clinical outcomes. Therefore, we hypothesized that epinephrine may induce unfavorable changes in gene expression of cardiomyocyte. Thus, we investigated effects of epinephrine exposure on the mediation or modulation of gene expression of cultured cardiomyocytes at a genome-wide scale. Our investigation revealed that exposure of cardiomyocytes to epinephrine in an in vitro environment can up-regulate the expression of angiopoietin-2 gene (+2...
September 2016: Journal of Biomedical Research
https://www.readbyqxmd.com/read/27831650/critical-genes-of-hepatocellular-carcinoma-revealed-by-network-and-module-analysis-of-rna-seq-data
#13
M-R Yang, Y Zhang, X-X Wu, W Chen
OBJECTIVE: RNA-seq data of hepatocellular carcinoma (HCC) was analyzed to identify critical genes related to the pathogenesis and prognosis. MATERIALS AND METHODS: Three RNA-seq datasets of HCC (GSE69164, GSE63863 and GSE55758) were downloaded from Gene Expression Omnibus (GEO), while another dataset including 54 HCC cases with survival time was obtained from The Cancer Genome Atlas (TCGA). Differentially expressed genes (DEGs) were identified by significant analysis of microarrays (SAM) method using package samr of R...
October 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27822069/sparc-is-a-possible-predictive-marker-for-albumin-bound-paclitaxel-in-non-small-cell-lung-cancer
#14
Kazutoshi Komiya, Tomomi Nakamura, Chiho Nakashima, Koichiro Takahashi, Hitomi Umeguchi, Naomi Watanabe, Akemi Sato, Yuji Takeda, Shinya Kimura, Naoko Sueoka-Aragane
OBJECTIVES: Nanoparticle albumin-bound paclitaxel (nab-paclitaxel) produced good tumor response in cases with lung squamous cell carcinoma, one of the most difficult cancers to treat. Secreted protein acidic and rich in cysteine (SPARC) binds to albumin, suggesting that SPARC plays an important role in tumor uptake of nab-paclitaxel. There is as yet no predictive marker for cytotoxic agents against non-small-cell lung cancer (NSCLC), and hence we believed that SPARC expression might be associated with tumor response to nab-paclitaxel...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27818162/identification-of-axon-growth-promoters-in-the-secretome-of-the-deer-antler-velvet
#15
Wolfgang Pita-Thomas, Gemma Barroso-García, Veronica Moral, Amber R Hackett, Valeria Cavalli, Manuel Nieto-Diaz
Every spring, deer cast their old antlers and initiate a regeneration process, which yields a new set of antlers of up to 1m in length. Over the course of three months, branches of the trigeminal nerve, originating from the frontal skull, innervate velvet, a modified skin that covers the regenerating antler. The rate of growth of these axons reaches up to 2cm per day making them the fastest regenerating axons in adult mammals. Here, we aim to identify the factors secreted by velvet that promote such high speed axon growth...
November 4, 2016: Neuroscience
https://www.readbyqxmd.com/read/27807289/the-regionalization-of-total-ankle-arthroplasties-and-ankle-fusions-in-new-york-state-a-10-year-comparative-analysis
#16
John A Buza, James X Liu, Jeffrey Jancuska, Joseph A Bosco
: Background Total ankle arthroplasty (TAA) provides an alternative to ankle fusion (AF). The purpose of this study is to (1) determine the extent of TAA regionalization, as well as examine the growth of TAA performed at high-, medium-, and low-volume New York State institutions and (2) compare this regionalization and growth with AF. Methods The New York Statewide Planning and Research Cooperative System (SPARCS) administrative data were used to identify 737 primary TAA and 7453 AF from 2005 to 2014...
November 1, 2016: Foot & Ankle Specialist
https://www.readbyqxmd.com/read/27802454/impact-of-sparc-expression-on-outcome-in-patients-with-advanced-pancreatic-cancer-not-receiving-nab-paclitaxel-a-pooled-analysis-from-prospective-clinical-and-translational-trials
#17
Steffen Ormanns, Michael Haas, Sibylle Baechmann, Annelore Altendorf-Hofmann, Anna Remold, Detlef Quietzsch, Michael R Clemens, Martin Bentz, Michael Geissler, Helmut Lambertz, Stephan Kruger, Thomas Kirchner, Volker Heinemann, Stefan Boeck
BACKGROUND: Conflicting results on the role of secreted protein acidic and rich in cysteins (SPARC) expression have been reported in resected pancreatic ductal adenocarcinoma (PDAC), and its prognostic and/or predictive role in advanced PDAC (aPDAC) has not been extensively investigated yet. This study was designed to evaluate SPARC expression as a biomarker in aPDAC patients (pts) not receiving nab-paclitaxel. METHODS: Using immunohistochemistry, we examined the stromal as well as the tumoral (i...
November 1, 2016: British Journal of Cancer
https://www.readbyqxmd.com/read/27798868/gene-expression-analysis-identifies-specific-patterns-of-dysregulated-molecular-pathways-and-genetic-subgroups-of-human-hepatocellular-carcinoma
#18
Holger G Hass, Ulrich Vogel, Michael Scheurlen, Jürgen Jobst
BACKGROUND: Hepatocellular carcinoma comprises of a group of heterogeneous tumors of different etiologies. The multistep process of liver carcinogenesis involves various genetic and phenotypic alterations. The molecular pathways and driver mutations involved are still under investigation. MATERIALS AND METHODS: DNA micorarray technology was used to identify differentially expressed genes between human hepatocarcinoma and non-tumorous liver tissues to establish a unique specific gene-expression profile independent of the underlying liver disease...
October 2016: Anticancer Research
https://www.readbyqxmd.com/read/27776337/secreted-protein-acidic-and-rich-in-cysteine-sparc-induces-lipotoxicity-in-neuroblastoma-by-regulating-transport-of-albumin-complexed-with-fatty-acids
#19
Alexandre Chlenski, Marija Dobratic, Helen R Salwen, Mark Applebaum, Lisa J Guerrero, Ryan Miller, Gillian DeWane, Elena Solomaha, Jeremy D Marks, Susan L Cohn
SPARC is a matrix protein that mediates interactions between cells and the microenvironment. In cancer, SPARC may either promote or inhibit tumor growth depending upon the tumor type. In neuroblastoma, SPARC is expressed in the stromal Schwannian cells and functions as a tumor suppressor. Here, we developed a novel in vivo model of stroma-rich neuroblastoma using non-tumorigenic SHEP cells with modulated levels of SPARC, mixed with tumorigenic KCNR cells. Tumors with stroma-derived SPARC displayed suppressed growth, inhibited angiogenesis and increased lipid accumulation...
October 20, 2016: Oncotarget
https://www.readbyqxmd.com/read/27772888/epithelial-sparc-expression-is-correlated-with-poor-survival-as-well-as-mmp-2-expression-of-invasive-breast-carcinoma
#20
Ji Shin Lee, Ga-Eon Kim, Jae Hyuk Lee, Jong Hee Nam, Chan Choi
No abstract text is available yet for this article.
February 2016: Pathology
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