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sperm capacitation physiology

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https://www.readbyqxmd.com/read/27901058/a-kinase-anchoring-protein-4-akap4-is-an-erk1-2-substrate-and-a-switch-molecule-between-camp-pka-and-pkc-erk1-2-in-human-spermatozoa
#1
Liat Rahamim Ben-Navi, Tal Almog, Zhong Yao, Rony Seger, Zvi Naor
Mammalian spermatozoa undergo capacitation and acrosome reaction in order to fertilize the egg. The PKC-ERK1/2 pathway plays an important role in human spermatozoa motility, capacitation and the acrosome reaction. Here we demonstrate that ERK1/2 phosphorylates proAKAP4 on Thr265 in human spermatozoa in vitro and in vivo. Cyclic AMP (cAMP) had no effect on ERK1/2 activity in human spermatozoa, but stimulated the MAPK in mouse pituitary LβT2 gonadotrope cells. cAMP via PKA attenuates PKC-dependent ERK1/2 activation only in the presence of proAKAP4...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27690295/factors-and-pathways-involved-in-capacitation-how-are-they-regulated
#2
Shi-Kai Jin, Wan-Xi Yang
In mammals, fertilization occurs via a comprehensive progression of events. Freshly ejaculated sperm have yet to acquire progressive motility or fertilization ability. They must first undergo a series of biochemical and physiological changes, collectively known as capacitation. Capacitation is a significant prerequisite to fertilization. During the process of capacitation, changes in membrane properties, intracellular ion concentration and the activities of enzymes, together with other protein modifications, induce multiple signaling events and pathways in defined media in vitro or in the female reproductive tract in vivo...
September 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/27678485/uterosome-like-vesicles-prompt-human-sperm-fertilising-capability
#3
A Franchi, M Cubilla, H A Guidobaldi, A A Bravo, L C Giojalas
STUDY QUESTION: Does the rapid transit through the uterine environment modulate the sperm physiological state? SUMMARY ANSWER: The uterosome-like vesicles (ULV) secreted by endometrial epithelial cells (EEC) in vitro are able to fuse with human spermatozoa, prompting their fertilising capacity. WHAT IS KNOWN ALREADY: Early studies suggest that sperm capacitation begins in the uterus and ends in the oviduct, and that a synergistic effect of both female organs may accelerate this process...
September 27, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27662642/sperm-binding-to-oviduct-epithelial-cells-enhances-tgfb1-and-il10-expressions-in-epithelial-cells-as-well-as-neutrophils-in-vitro-prostaglandin-e2-as-a-main-regulator-of-anti-inflammatory-response-in-the-bovine-oviduct
#4
Mohamed Samy Yousef, Mohamed Ali Marey, Nina Hambruch, Hiroyuki Hayakawa, Takashi Shimizu, Hassan Ali Hussien, Abdel-Razek Khalifa Abdel-Razek, Christiane Pfarrer, Akio Miyamoto
Sperm are allogenic to the female genital tract; however, oviducts provide optimal conditions for survival and capacitation of these non-self cells until fertilization. Recently, we showed that oviduct-conditioned media and prostaglandin E2 (PGE2) suppress sperm phagocytosis by polymorphonuclear neutrophils (PMNs) under physiological conditions. We hypothesized that sperm binding to bovine oviduct epithelial cells (BOECs) could change the local innate immunity via PGE2. As the first step to obtain basic information, sub-confluent BOEC monolayers were co-cultured with swim-up sperm for 2 h...
2016: PloS One
https://www.readbyqxmd.com/read/27651517/why-doesn-t-conventional-ivf-work-in-the-horse
#5
Bart Leemans, Bart M Gadella, Tom Arjun Edgar Stout, Catharina De Schauwer, Hilde Maria Nelis, Maarten Hoogewijs, Ann Van Soom
In contrast to man and many other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. The apparent inability of stallion spermatozoa to penetrate the zona pellucida in vitro is most likely due to incomplete activation of spermatozoa (capacitation) as a result of inadequate capacitating or fertilizing media. In vivo, the oviduct and its secretions provide a micro-environment that does reliably support and regulate interaction between the gametes. This review focuses on equine sperm-oviduct interaction...
September 20, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27625611/the-calcium-sensing-receptor-and-the-reproductive-system
#6
REVIEW
Isabella Ellinger
Active placental transport of maternal serum calcium (Ca(2+)) to the offspring is pivotal for proper development of the fetal skeleton as well as various organ systems. Moreover, extracellular Ca(2+) levels impact on distinct processes in mammalian reproduction. The calcium-sensing receptor (CaSR) translates changes in extracellular Ca(2+)-concentrations into cellular reactions. This review summarizes current knowledge on the expression of CaSR and its putative functions in reproductive organs. CaSR was detected in placental cells mediating materno-fetal Ca(2+)-transport such as the murine intraplacental yolk sac (IPYS) and the human syncytiotrophoblast...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27599714/ouabain-interactions-with-the-%C3%AE-4-isoform-of-the-sodium-pump-trigger-non-classical-steroid-hormone-signaling-and-integrin-expression-in-spermatogenic-cells
#7
Neha Upmanyu, Raimund Dietze, Ulrike Kirch, Georgios Scheiner-Bobis
In addition to the ubiquitous α1 isoform of the sodium pump, sperm cells also express a male-specific α4 isoform whose function has been associated with sperm motility, fertility, and capacitation. Here we investigate in the murine spermatogenic cell line GC-2 interactions of the α4 isoform with the cardiotonic steroid ouabain in signaling cascades involved in the non-classical action of steroid hormones. Exposure of GC-2 cells to low concentrations of ouabain stimulates the phosphorylation of Erk1/2 and of the transcription factors CREB and ATF-1...
November 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27565610/characterization-of-camp-phosphodiesterase-activity-in-bovine-seminal-plasma
#8
A Bergeron, J P Aragon, C Guillemette, A Hébert, R Sullivan, P Blondin, F J Richard
The second messenger cyclic adenosine monophosphate (cAMP) has a central role in sperm physiology. Extracellular cAMP can be sequentially degraded into 5'AMP and adenosine by ecto-phosphodiesterases (ecto-PDE) and ecto-nucleotidases, a phenomenon called extracellular cAMP-adenosine pathway. As cAMP-adenosine pathway is involved in sperm capacitation, we hypothesize that extracellular PDEs are functionally present in seminal plasma. Exclusively measuring cAMP-PDE activity, total activity in bovine seminal plasma was 10...
August 27, 2016: Andrology
https://www.readbyqxmd.com/read/27526775/mrna-expression-pattern-of-selected-candidate-genes-differs-in-bovine-oviductal-epithelial-cells-in-vitro-compared-with-the-in-vivo-state-and-during-cell-culture-passages
#9
Sadjad Danesh Mesgaran, Jutta Sharbati, Ralf Einspanier, Christoph Gabler
BACKGROUND: The mammalian oviduct provides the optimal environment for gamete maturation including sperm capacitation, fertilization, and development of the early embryo. Various cell culture models for primary bovine oviductal epithelial cells (BOEC) were established to reveal such physiological events. The aim of this study was to evaluate 17 candidate mRNA expression patterns in oviductal epithelial cells (1) in transition from in vivo cells to in vitro cells; (2) during three consecutive cell culture passages; (3) affected by the impact of LOW or HIGH glucose content media; and (4) influenced by different phases of the estrous cycle in vivo and in vitro...
2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27512123/oviductal-response-to-gametes-and-early-embryos-in-mammals
#10
REVIEW
Veronica Maillo, Maria Jesus Sánchez-Calabuig, Ricaurte Lopera-Vasquez, Meriem Hamdi, Alfonso Gutierrez-Adan, Patrick Lonergan, Dimitrios Rizos
The oviduct is a complex and organized thin tubular structure connecting the ovary with the uterus. It is the site of final sperm capacitation, oocyte fertilization and, in most species, the first 3-4days of early embryo development. The oviductal epithelium is made up of ciliary and secretory cells responsible for the secretion of proteins and other factors which contribute to the formation of the oviductal fluid. Despite significant research, most of the pathways and oviductal factors implicated in the crosstalk between gametes/early embryo and the oviduct remain unknown...
October 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27491678/oestrous-sheep-serum-balances-ros-levels-to-supply-in-vitro-capacitation-of-ram-spermatozoa
#11
E Del Olmo, O García-Álvarez, A Maroto-Morales, M Ramón, M Iniesta-Cuerda, F Martinez-Pastor, V Montoro, A J Soler, J J Garde, M R Fernández-Santos
Reactive oxygen species (ROS) are fundamental for intracellular signalling. In spermatozoa, they are involved both to apoptosis and to capacitation, and changes in ROS levels can alter the balance between these two processes. Oestrous sheep serum (OSS) is considered an efficient agent for in vitro capacitation of ram spermatozoa. We have explored the effects of OSS on ram sperm physiology, especially on ROS production, during in vitro capacitation. Semen samples from 15 rams were cryopreserved. After thawing, samples were submitted to four treatments: control (CTL), 10% OSS supplementation for in vitro sperm capacitation, caspase inhibitor (INH, Z-VAD-FMK 100 μM) and OSS (10%) plus caspase inhibitor (I + E)...
October 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27345329/angiotensin-ii-increases-sperm-phagocytosis-by-neutrophils-in-vitro-a-possible-physiological-role-in-the-bovine-oviduct
#12
Mohamed Ali Marey, Mohamed Samy Yousef, Jinghui Liu, Kazuhiro Morita, Motoki Sasaki, Hiroyuki Hayakawa, Takashi Shimizu, Akio Miyamoto
This study aimed to investigate the possible effects of the vasoactive peptide angiotensin II (ANG II), secreted by bovine oviduct epithelial cells, on the in vitro phagocytic activity of polymorphonuclear leukocytes, specifically neutrophils, towards sperm. The measured concentrations of ANG II in oviduct flushes and conditioned medium from primary bovine oviduct epithelial culture ranged from 10(-10) to 10(-11)  M. In our experiments, neutrophils were incubated for 2 hr with ANG II (0, 10(-11) , 10(-10) , 10(-9) , and 10(-8)  M)...
July 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27194353/role-of-actin-cytoskeleton-during-mammalian-sperm-acrosomal-exocytosis
#13
REVIEW
Ana Romarowski, Guillermina M Luque, Florenza A La Spina, Dario Krapf, Mariano G Buffone
Mammalian sperm require to undergo an exocytotic process called acrosomal exocytosis in order to be able to fuse with the oocyte. This ability is acquired during the course of sperm capacitation. This review is focused on one aspect related to this acquisition: the role of the actin cytoskeleton. Evidence from different laboratories indicates that actin polymerization occurs during capacitation, and the detection of several actin-related proteins suggests that the cytoskeleton is involved in important sperm functions...
2016: Advances in Anatomy, Embryology, and Cell Biology
https://www.readbyqxmd.com/read/27194351/sperm-capacitation-and-acrosome-reaction-in-mammalian-sperm
#14
REVIEW
Cintia Stival, Lis del C Puga Molina, Bidur Paudel, Mariano G Buffone, Pablo E Visconti, Dario Krapf
Physiological changes that endow mammalian sperm with fertilizing capacity are known as sperm capacitation. As part of capacitation, sperm develop an asymmetrical flagellar beating known as hyperactivation and acquire the ability to undergo the acrosome reaction. Together, these processes promote fertilizing competence in sperm. At the molecular level, capacitation involves a series of signal transduction events which include activation of cAMP-dependent phosphorylation pathways, removal of cholesterol, hyperpolarization of the sperm plasma membrane, and changes in ion permeability...
2016: Advances in Anatomy, Embryology, and Cell Biology
https://www.readbyqxmd.com/read/27071965/modulation-of-human-sperm-capacitation-by-progesterone-estradiol-and-luteinizing-hormone
#15
Aideé Saray López-Torres, Mayel Chirinos
Sperm residency in female reproductive tract is essential to undergo functional changes that allow the cell to encounter the oocyte and fertilize it. Those changes, known as capacitation, are modulated by molecules located in the uterotubal surface and fluids. During the fertile window, there is a notable increase in some reproductive hormones such as progesterone, estradiol, and luteinizing hormone in the female reproductive tract, so spermatozoa are exposed to these hormones in an environment that must favor gamete encountering and fusion...
April 12, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27062907/post-translational-modifications-in-sperm-proteome-the-chemistry-of-proteome-diversifications-in-the-pathophysiology-of-male-factor-infertility
#16
REVIEW
Luna Samanta, Nirlipta Swain, Ahmet Ayaz, Vijay Venugopal, Ashok Agarwal
BACKGROUND: The spermatozoa undergo a series of changes in the epididymis to mature after their release from the testis and subsequently in the female reproductive tract after ejaculation to get capacitated and achieve fertilization potential. Despite having a silenced protein synthesis machinery, the dynamic change in protein profile of the spermatozoa is attributed either to acquisition of new proteins via vescicular transport or to several post-translational modifications (PTMs) occurring on the already expressed protein complement...
July 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27053366/regulation-of-sperm-capacitation-by-the-26s-proteasome-an-emerging-new-paradigm-in-spermatology
#17
Karl Kerns, Patricio Morales, Peter Sutovsky
The ubiquitin proteasome system (UPS) participates in many biological processes ranging from cell cycle and antigen processing to cellular defense and signaling. Work of the last decade has made it evident that the UPS is involved in many sperm-related processes leading up to and as part of fertilization. The current knowledge of UPS involvement and changes during sperm capacitation are reviewed together with a list of known proteasome-associated sperm proteins and a discussion of the relationships between these proteins and the proteasome...
May 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27048852/prion-protein-2-dublet-gene-prnd-role-in-ovine-semen-capacitation-cryopreservation-and-fertility
#18
L M Ferreira, M Garcia-Herreros, A Domingos, C C Marques, P Mesquita, J P Barbas, M C Baptista, J Pimenta, A E M Horta, J A M Prates, R M L N Pereira
The aim of the present study was to examine the role of Doppel protein in the capacitation process and fertilising ability of both fresh and frozen-thawed (FT) spermatozoa from rams carrying different prion protein 2 (dublet) (PRND) gene polymorphisms. The detection efficacy of new anti-Doppel monoclonal antibodies and PRND mRNA quantification were also explored in ovine spermatozoa. Three different genotypes (AA, GA, GG) were identified for codon 26 of ovine PRND-c.78G>A. Using flow cytometry, a higher fluorescence was detected in fresh compared with FT sperm samples incubated with anti-Doppel primary and fluorescein isothiocyanate-conjugated secondary antibodies (P < 0...
April 6, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27037571/selection-of-physiological-spermatozoa-during-intracytoplasmic-sperm-injection
#19
B Torki-Boldaji, M Tavalaee, M Bahadorani, M H Nasr-Esfahani
Sperm genomic integrity has a significant effect on intracytoplasmic sperm injection (ICSI) outcomes, especially post-implantation. Spermatozoa selected based on motility and morphology do not guarantee the genomic integrity of spermatozoa. Nearly fifty percentage of spermatozoa in infertile men with normal morphology present different degrees of DNA fragmentation. However, capacitated or hyperactivated spermatozoa show lower degrees of DNA fragmentation. Therefore, selection of hyperactivated spermatozoa may improve ICSI outcome...
April 1, 2016: Andrologia
https://www.readbyqxmd.com/read/26960363/identification-localization-and-functional-analysis-of-the-homologues-of-mouse-cabs1-protein-in-porcine-testis
#20
Hossam H Shawki, Takumi Kigoshi, Yuki Katoh, Manabu Matsuda, Chioma M Ugboma, Satoru Takahashi, Hisashi Oishi, Akihiro Kawashima
Previously, we have identified a calcium-binding protein that is specifically expressed in spermatids and localized to the flagella of the mature sperm in mouse, so-called mCABS1. However, the physiological roles of CABS1 in the male reproductive system have not been fully elucidated yet. In the current study, we aimed to localize and clarify the role of CABS1 in porcine (pCABS1). We determined for the first time the full nucleotides sequence of pCABS1 mRNA. pCABS1 protein was detected on SDS-PAGE gel as two bands at 75 kDa and 70 kDa in adult porcine testis, whereas one band at 70 kDa in epididymal sperm...
July 29, 2016: Experimental Animals
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