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sperm capacitation

Valentina Longobardi, Angela Salzano, Giuseppe Campanile, Raffaele Marrone, Francesco Palumbo, Milena Vitiello, Gianluigi Zullo, Bianca Gasparrini
The aim of this study was to evaluate the effect of carnitine supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. Buffalo semen was cryopreserved in BioXcell containing 0 (control group), 2.5 and 7.5-mM carnitine. After thawing, viability, motility, membrane integrity and capacitation status (assessed by localization of phosphotyrosine-containing proteins and chlortetracycline, chlortetracycline assay) were evaluated. Furthermore, total antioxidant capacity, reactive oxygen species (ROS) and lipid peroxidation levels, as well as adenosine triphosphate (ATP) content and phospholipids concentration were assessed...
September 20, 2016: Theriogenology
Lis C Puga Molina, Nicolás A Pinto, Paulina Torres Rodríguez, Ana Romarowski, Alberto Vicens Sanchez, Pablo E Visconti, Alberto Darszon, Claudia L Treviño, Mariano G Buffone
Mammalian sperm require to spend a limited period of time in the female reproductive tract to become competent to fertilize in a process called capacitation. It is well established that HCO3(-) is essential for capacitation because it activates the atypical soluble adenylate cyclase ADCY10 leading to cAMP production, and promotes alkalinization of cytoplasm and membrane hyperpolarization. However, how HCO3(-) is transported into the sperm is not well understood. There is evidence that CFTR activity is involved in the human sperm capacitation but how this channel is integrated in the complex signaling cascades associated with this process remains largely unknown...
October 7, 2016: Journal of Cellular Physiology
Beatriz Fernandez-Fuertes, Fernando Narciandi, Cliona O'Farrelly, Alan K Kelly, Sean Fair, Kieran G Meade, Patrick Lonergan
Bovine beta-defensin 126 (BBD126) exhibits preferential expression for the cauda epididymis of males, where it is absorbed onto the tail and post acrosomal region of sperm. The aim of this study was to examine the role of BBD126 in bull sperm function. Fresh and frozen-thawed semen were incubated in the presence of different capacitating agents, as well as with phosphatidylinositol-specific phospholipase C. These treatments, which have been successful in releasing beta-defensin 126 from macaque sperm, proved to be ineffective in bull sperm...
October 5, 2016: Biology of Reproduction
W L Flowers, F Deller, K R Stewart
The objective of this study was to evaluate relationships between common semen quality estimates including sperm motility, sperm morphology, spontaneous capacitation status and seminal plasma proteins and boar fertility using heterospermic inseminations and subsequent paternity testing. All boars (n=12) used in the study had excellent semen quality (≥70% normal sperm) that resulted in average farrowing rates and litter sizes of 88.9±0.7% and 11.7±0.1 pigs, respectively. Their ejaculates were combined to make heterospermic insemination doses in such a way that each boar was tested against all of his contemporaries...
September 29, 2016: Animal Reproduction Science
Shi-Kai Jin, Wan-Xi Yang
In mammals, fertilization occurs via a comprehensive progression of events. Freshly ejaculated sperm have yet to acquire progressive motility or fertilization ability. They must first undergo a series of biochemical and physiological changes, collectively known as capacitation. Capacitation is a significant prerequisite to fertilization. During the process of capacitation, changes in membrane properties, intracellular ion concentration and the activities of enzymes, together with other protein modifications, induce multiple signaling events and pathways in defined media in vitro or in the female reproductive tract in vivo...
September 27, 2016: Oncotarget
Jana Jankovicova, Michaela Frolikova, Natasa Sebkova, Michal Simon, Petra Cupperova, Denisa Lipcseyova, Katarina Michalkova, Lubica Horovska, Radislav Sedlacek, Pavel Stopka, Jana Antalikova, Katerina Dvorakova-Hortova
Sperm-egg interaction and fusion represents a key moment of fertilization and it would not happen in mammals without the interaction of the tetraspanin superfamily members including protein CD81. A detailed immunohistochemical localization of CD81 was monitored on bovine oocytes of different maturation stages, as well as during early embryogenesis. There was also a CD81characterization on bovine and mouse sperm carried out. On bovine eggs, CD81 was detected on the plasma membrane of the germinal vesicle, metaphase I and metaphase II oocytes...
September 27, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
A Franchi, M Cubilla, H A Guidobaldi, A A Bravo, L C Giojalas
STUDY QUESTION: Does the rapid transit through the uterine environment modulate the sperm physiological state? SUMMARY ANSWER: The uterosome-like vesicles (ULV) secreted by endometrial epithelial cells (EEC) in vitro are able to fuse with human spermatozoa, prompting their fertilising capacity. WHAT IS KNOWN ALREADY: Early studies suggest that sperm capacitation begins in the uterus and ends in the oviduct, and that a synergistic effect of both female organs may accelerate this process...
September 27, 2016: Molecular Human Reproduction
Vidisha Minhas, Abhinav Shrestha, Neerja Wadhwa, Rita Singh, Satish Kumar Gupta
Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino acid residues 76-126) terminal fragments of mouse sperm protein 17 (Sp17) fused to the promiscuous T non-B cell epitope of tetanus toxoid (TT), amino acid residues 830-844 followed by di-lysine linker (KK) (TT-KK-Sp17N or TT-KK-Sp17C , respectively) or (ii) mouse equatorin (amino acid residues 21-185) fused to the T non-B cell epitope of bovine RNase (amino acid residues 94-104) were expressed in Escherichia coli...
September 27, 2016: Molecular Reproduction and Development
Mariangela Palmieri, Palma Papale, Antonietta Della Ragione, Giuseppa Quaranta, Giovanni Russo, Sabatino Russo
Male infertility and the poor quality of sperm seem to be influenced by oxidative stress. In particular, the reactive oxygen species (ROS) mainly produced by morphologically altered spermatozoa affect sperm motility, morphology, and integrity. The aim of this study was to evaluate the efficacy of Myo-Inositol (Myo-Ins) on a number of parameters such as viscosity and total and progressive motility of spermatozoa, in order to better validate its possible practical application in vitro, in order to improve the capacitation protocols commonly used in Assisted Reproductive Technology (ART)...
2016: International Journal of Endocrinology
Mohamed Samy Yousef, Mohamed Ali Marey, Nina Hambruch, Hiroyuki Hayakawa, Takashi Shimizu, Hassan Ali Hussien, Abdel-Razek Khalifa Abdel-Razek, Christiane Pfarrer, Akio Miyamoto
Sperm are allogenic to the female genital tract; however, oviducts provide optimal conditions for survival and capacitation of these non-self cells until fertilization. Recently, we showed that oviduct-conditioned media and prostaglandin E2 (PGE2) suppress sperm phagocytosis by polymorphonuclear neutrophils (PMNs) under physiological conditions. We hypothesized that sperm binding to bovine oviduct epithelial cells (BOECs) could change the local innate immunity via PGE2. As the first step to obtain basic information, sub-confluent BOEC monolayers were co-cultured with swim-up sperm for 2 h...
2016: PloS One
Bart Leemans, Bart M Gadella, Tom Arjun Edgar Stout, Catharina De Schauwer, Hilde Maria Nelis, Maarten Hoogewijs, Ann Van Soom
In contrast to man and many other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. The apparent inability of stallion spermatozoa to penetrate the zona pellucida in vitro is most likely due to incomplete activation of spermatozoa (capacitation) as a result of inadequate capacitating or fertilizing media. In vivo, the oviduct and its secretions provide a micro-environment that does reliably support and regulate interaction between the gametes. This review focuses on equine sperm-oviduct interaction...
September 20, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Diego Bucci, Elisa Giaretta, Barbara Merlo, Eleonora Iacono, Marcella Spinaci, Beatrice Gadani, Gaetano Mari, Carlo Tamanini, Giovanna Galeati
Alkaline phosphatase (AP) is present in equine seminal plasma and spermatozoa, but its functional role is not fully understood yet. Being that, sperm-oocyte interaction in equine species has been reported to be enhanced at a slightly basic pH, this work aimed at verifying whether exogenous alkaline phosphatase exerts any role on stallion spermatozoa and sperm-oocyte interaction at different pHs (7.4; 8.0; 9.0). Stallion spermatozoa were capacitated in Tyrode's medium at pH 7.4, 8.0, and 9.0 for 4 hours at 38 °C, 5% CO2 with 2...
August 13, 2016: Theriogenology
Felipe A Navarrete, Antonio Alvau, Hoi Chang Lee, Lonny R Levin, Jochen Buck, Patricia Martin-De Leon, Celia M Santi, Dario Krapf, Jesse Mager, Rafael A Fissore, Ana M Salicioni, Alberto Darszon, Pablo E Visconti
Mammalian sperm acquire fertilizing capacity in the female tract in a process called capacitation. At the molecular level, capacitation requires protein kinase A activation, changes in membrane potential and an increase in intracellular calcium. Inhibition of these pathways results in loss of fertilizing ability in vivo and in vitro. We demonstrated that transient incubation of mouse sperm with Ca(2+) ionophore accelerated capacitation and rescued fertilizing capacity in sperm with inactivated PKA function...
2016: Scientific Reports
Isabella Ellinger
Active placental transport of maternal serum calcium (Ca(2+)) to the offspring is pivotal for proper development of the fetal skeleton as well as various organ systems. Moreover, extracellular Ca(2+) levels impact on distinct processes in mammalian reproduction. The calcium-sensing receptor (CaSR) translates changes in extracellular Ca(2+)-concentrations into cellular reactions. This review summarizes current knowledge on the expression of CaSR and its putative functions in reproductive organs. CaSR was detected in placental cells mediating materno-fetal Ca(2+)-transport such as the murine intraplacental yolk sac (IPYS) and the human syncytiotrophoblast...
2016: Frontiers in Physiology
Sandra L Gray, Brett R Lackey, William R Boone
BACKGROUND: Estrogen signaling pathways are modulated by exogenous factors. Panax ginseng exerts multiple activities in biological systems and is classified as an adaptogen. Zearalenol is a potent mycoestrogen that may be present in herbs and crops arising from contamination or endophytic association. The goal of this study was to investigate the impact of P. ginseng, zearalenol and estradiol in tests on spermatozoal function. METHODS: The affinity of these compounds for estrogen receptor (ER)-alpha and beta (ERα and ERβ)-was assessed in receptor binding assays...
July 2016: Journal of Ginseng Research
Neha Upmanyu, Raimund Dietze, Ulrike Kirch, Georgios Scheiner-Bobis
In addition to the ubiquitous α1 isoform of the sodium pump, sperm cells also express a male-specific α4 isoform whose function has been associated with sperm motility, fertility, and capacitation. Here we investigate in the murine spermatogenic cell line GC-2 interactions of the α4 isoform with the cardiotonic steroid ouabain in signaling cascades involved in the non-classical action of steroid hormones. Exposure of GC-2 cells to low concentrations of ouabain stimulates the phosphorylation of Erk1/2 and of the transcription factors CREB and ATF-1...
September 3, 2016: Biochimica et Biophysica Acta
Jean-Ju Chung
The'sugarcoat', a dense carbohydrate coat (glycocalyx), is critical to cell-to-cell recognition, including sperm-egg interactions. Lectins are used to probe the localization of glycoproteins on the cell surface, and to track their changes during sperm maturation and capacitation (Tecle and Gagneux, 2015, Mol. Reprod. Dev 82:635-650). Among them, wheat germ agglutinin (WGA) binds to the sperm head, cytoplasmic droplet, midpiece, as well as the principal piece and end piece of the sperm tail (Fabrega et al, 2012, Reprod...
September 3, 2016: Molecular Reproduction and Development
Erif Maha Nugraha Setyawan, Min Jung Kim, Hyun Ju Oh, Geon A Kim, Young Kwang Jo, Seok Hee Lee, Yoo Bin Choi, Byeong Chun Lee
The objective of this study was to determine the ability of spermine to act as an antioxidant in scavenging reactive oxygen species (ROS), maintaining sperm function and decreasing cryocapacitation after cryopreservation. Although motility did not increase with spermine treatment, values for membrane integrity were significantly increased (P < 0.05). Higher percentages of linearity and straightness with a lower amplitude of lateral head displacement (ALH) indicated that spermine inhibits hyperactivation...
October 28, 2016: Biochemical and Biophysical Research Communications
Yuewen Zhao, Xiaojing Yang, Zongchao Jia, Robert L Reid, Pierre Leclerc, Frederick W K Kan
The mammalian oviduct synthesizes and secretes a major glycoprotein known as oviductin (OVGP1) which has been shown to interact with gametes and early embryos. We report here the use of recombinant DNA technology to produce, for the first time, the secretory form of human OVGP1 in HEK293 cells. HEK293 colonies stably expressing recombinant human OVGP1 (rHuOVGP1) were established by transfecting cells with an expression vector pCMV6-Entry constructed with OVGP1 cDNA. Large quantities of rHuOVGP1 were obtained from the stably transfected cells using the CELLSPIN cell cultivation system...
August 31, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Chun-Jin Li, Dong Wang, Xu Zhou
Sperm is highly differentiated cell that can be easily obtained and purified. Mature sperm is considered to be transcriptionally and translationally silent and incapable of protein synthesis. Recently, a large number of proteins have been identified in sperm from different species by using the proteomic approaches. Clinically, sperm proteins can be used as markers for male infertility due to different protein profiles identified in sperm from fertile and infertile male animals. Recent evidences have shown that the conditions of sperm preservation in vitro can also change the sperm protein profiles...
October 2016: Animal Reproduction Science
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