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sperm capacitation

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https://www.readbyqxmd.com/read/28894123/mutations-in-the-prostate-specific-antigen-psa-klk3-correlate-with-male-infertility
#1
Nishi Gupta, Digumarthi V S Sudhakar, Pravin Kumar Gangwar, Satya Narayan Sankhwar, Nalini J Gupta, Baidyanath Chakraborty, Kumarasamy Thangaraj, Gopal Gupta, Singh Rajender
Prostate specific antigen (PSA/KLK3) is known to be the chief executor of the fragmentation of semenogelins, dissolution of semen coagulum, thereby releasing sperm for active motility. Recent research has found that semenogelins also play significant roles in sperm fertility by affecting hyaluronidase activity, capacitation and motility, thereby making PSA important for sperm fertility beyond simple semen liquefaction. PSA level in semen has been shown to correlate with sperm motility, suggesting that PSA level/activity can affect fertility...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28887327/uterine-flushings-from-women-treated-with-levonorgestrel-affect-sperm-functionality-in-vitro
#2
Mayel Chirinos, Marta Durand, María Elena González-González, Gabriela Hernández-Silva, Israel Maldonado-Rosas, Pablo Lopez, Fernando Larrea
Levonorgestrel (LNG), a synthetic 19 nor-testosterone derivative, is widely used for emergency contraception. It is well known that LNG prevents ovulation only when given prior to the surge of serum luteinizing hormone (LH) during the periovulatory phase of the menstrual cycle. This observation suggests that LNG, given its contraceptive efficacy, has additional effects other than those affecting ovulation. In this study, we have evaluated the effects on human sperm functionality of uterine flushings (UF) obtained from women at day LH+1 of a control cycle (CTR-LH+1) and after receiving LNG (LNG-LH+1) two days before the surge of LH...
September 8, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28883914/effect-of-recombinant-and-native-buffalo-ovgp1-on-sperm-functions-and-in-vitro-embryo-development-a-comparative-study
#3
Suman Choudhary, A Kumaresan, Manish Kumar, Shivani Chhillar, Hrudananda Malik, Sudarshan Kumar, Jai K Kaushik, Tirtha K Datta, Ashok K Mohanty
BACKGROUND: An oviduct- specific glycoprotein, OVGP1, is synthesized and secreted by non-ciliated epithelial cells of the mammalian oviduct which provides an essential milieu for reproductive functions. The present study reports the effects of recombinant buffalo OVGP1 that lacks post-translational modifications, and native Buffalo OVGP1 isolated from oviductal tissue, on frozen- thawed sperm functions and in vitro embryo development. RESULTS: The proportion of viable sperms was greater (P < 0...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28859280/subfertility-in-bulls-carrying-a-nonsense-mutation-in-transmembrane-protein-95-is-due-to-failure-to-interact-with-the-oocyte-vestments
#4
Beatriz Fernandez-Fuertes, Ricardo Laguna-Barraza, Raul Fernandez-Gonzalez, Alfonso Gutierrez-Adan, Alfonso Blanco-Fernandez, Alan M O'Doherty, Mauro Di Fenza, Alan K Kelly, Sabine Kölle, Patrick Lonergan
In a recent genome-wide association study, 40 Fleckvieh bulls with exceptionally poor fertility were found to be homozygous for a nonsense mutation in the transmembrane protein 95 (TMEM95) encoding gene. Ejaculates from these individuals exhibited normal sperm concentration, morphology, viability, and motility. However, only 1.7% of inseminations resulted in pregnancies. The aim of this study was to examine the effect of this mutation in TMEM95 on bovine sperm function in vitro. Sperm from homozygous (mt/mt) males had lower in vitro fertility than sperm from wild-type (wt/wt) or heterozygous (wt/mt) bulls (P < 0...
July 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28859152/elevated-aminopeptidase-n-affects-sperm-motility-and-early-embryo-development
#5
Amena Khatun, Md Saidur Rahman, Do-Yeal Ryu, Woo-Sung Kwon, Myung-Geol Pang
Aminopeptidase N (APN) is a naturally occurring ectopeptidase present in mammalian semen. Previous studies have demonstrated that APN adversely affects male fertility through the alteration of sperm motility. This enzyme constitutes 0.5 to 1% of the seminal plasma proteins, which can be transferred from the prostasomes to sperms by a fusion process. In the present study, we investigated the molecular mechanism of action of APN and its role in regulating sperm functions and male fertility. In this in vitro study, epididymal mouse spermatozoa were incubated in a capacitating media (pH 7) containing 20 ng/mL of recombinant mouse APN for 90 min...
2017: PloS One
https://www.readbyqxmd.com/read/28854585/sperm-chemorepulsion-a-supplementary-mechanism-to-regulate-fertilization
#6
H A Guidobaldi, M Cubilla, A Moreno, M V Molino, L Bahamondes, L C Giojalas
STUDY QUESTION: Are human spermatozoa able of chemorepulsive behaviour? SUMMARY ANSWER: Capacitated human spermatozoa are able to be chemorepelled by synthetic Progesterone Receptor Ligands (sPRL, known as contraceptives) and zinc (a cation released by the oocyte upon fertilization). WHAT IS KNOWN ALREADY: Moving cells can be oriented towards or against a molecular gradient, processes called chemoattraction and chemorepulsion, respectively, which have been described in unicellular organisms such as amoebas and bacteria, to organismic cells such macrophages and developmental cells...
August 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28845020/is-transcription-in-sperm-stationary-or-dynamic
#7
Xiaoxia Ren, Xiaoli Chen, Zhenling Wang, Dong Wang
Transcriptional activity is repressed due to the packaging of sperm chromatins during spermiogenesis. The detection of numerous transcripts in sperm, however, raises the question whether transcriptional events exist in sperm, which has been the central focus of the recent studies. To summarize the transcriptional activity during spermiogenesis and in sperm, we reviewed the documents on transcript differences during spermiogenesis, in sperm with differential motility, before and after capacitation and cryopreservation...
August 28, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28833824/effects-of-latrunculin-a-on-the-relocation-of-sperm-izumo1-during-gamete-interaction-in-the-mouse
#8
Chong Zhou, Li Huang, De-Shun Shi, Jian-Rong Jiang
The sperm protein IZUMO1 plays a central role in gamete fusion. In mouse sperm, IZUMO1 is enriched at the acrosomal cap before the acrosome reaction, and at the equatorial segment following this reaction; its relocation is dependent on filamentous actin. How actin polymerization affects IZUMO1 relocation during gamete interaction remains unknown. The present study addressed these processes using latrunculin A (LatA), an inhibitor of actin polymerization. We report that 25 µM LatA blocked actin polymerization in the capacitated sperm head, resulting in a marked decrease in sperm with relocated IZUMO1 during the A23187-induced acrosome reaction and cumulus layer penetration...
August 18, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28833817/na-k-atpase-regulates-bovine-sperm-capacitation-through-raft-and-non-raft-mediated-signaling-mechanisms
#9
Gayathri D Rajamanickam, John P Kastelic, Jacob C Thundathil
Highly dynamic lipid microdomains (rafts) in the sperm plasma membrane contain several signaling proteins that regulate sperm capacitation. Na/K-ATPase isoforms (testis-specific isoform ATP1A4 and ubiquitous isoform ATP1A1) are abundant in bovine sperm plasma membrane. We previously reported that incubation of bovine sperm with ouabain, a specific Na/K-ATPase ligand, induced tyrosine phosphorylation of several sperm proteins during capacitation. The objective of this study was to investigate the roles of lipid rafts and non-rafts in Na/K-ATPase enzyme activity and signaling during bovine sperm capacitation...
August 19, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28810916/the-catsper-channel-and-its-roles-in-male-fertility-a-systematic-review
#10
REVIEW
Xiang-Hong Sun, Ying-Ying Zhu, Lin Wang, Hong-Ling Liu, Yong Ling, Zong-Li Li, Li-Bo Sun
The Catsper channel is a sperm-specific, Ca(2+)-permeable, pH-dependent, and low voltage-dependent channel that is essential for the hyperactivity of sperm flagellum, chemotaxis towards the egg, capacitation and acrosome reaction. All of these physiological events require calcium entry into sperm cells. Remarkably, Catsper genes are exclusively expressed in the testis during spermatogenesis, and are sensitive to ion channel-induced pH change, such as NHEs, Ca(2+)ATPase, K(+) channel, Hv1 channel and HCO3(-) transporters...
August 15, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28794880/relationship-between-semenogelins-bound-to-human-sperm-and-other-semen-parameters-and-pregnancy-outcomes
#11
Kazumitsu Yamasaki, Kaoru Yoshida, Miki Yoshiike, Kazuhiko Shimada, Hiroyuki Nishiyama, Satoru Takamizawa, Kaoru Yanagida, Teruaki Iwamoto
BACKGROUND: Semenogelins (SEMGs) are major components of human seminal vesicle secretions. Due to SEMG's sperm-motility inhibitor, a significant negative correlation between sperm motility and the proportion of SEMG-bound spermatozoa (SEMG+) was found in asthenozoospermic patients. SEMGs also show intrinsic inhibitory capability for sperm capacitation; however, studies on actual clinical specimens have not been conducted. METHODS: To reveal the relationship between SEMGs and the fertilizing capacity of sperm from male infertile patients who are not restricted to asthenozoospermia, we measured the proportion of SEMG+ in the spermatozoa of 142 male infertile patients...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28770098/boar-management-and-semen-handling-factors-affect-the-quality-of-boar-extended-semen
#12
REVIEW
Alfonso Lopez Rodriguez, Ann Van Soom, Ioannis Arsenakis, Dominiek Maes
Artificial insemination (AI) is the preferred method for reproduction in the majority of the intensive pig production systems Worldwide. To this end, fresh extended ready-to-use semen doses are either purchased from AI-centres or produced by boars kept on-farm. For profitable semen production, it is necessary to obtain a maximum amount of high quality semen from each boar. This paper reviews current knowledge on factors that may affect semen quality by influencing the boar or the semen during processing. Genetic markers could be used for early detection of boars with the highest fertility potential...
2017: Porcine Health Management
https://www.readbyqxmd.com/read/28762521/sperm-cystic-fibrosis-transmembrane-conductance-regulator-expression-level-is-relevant-to-fecundity-of-healthy-couples
#13
P-B Sun, H-M Xu, K Li, H-C Li, A-J Chen, M-J Chen, H-T Dai, Y Ni
Cystic fibrosis transmembrane conductance regulator (CFTR) is relevant to sperm quality, sperm capacitation and male fertility. However, it is still unknown whether CFTR can be a potential parameter for fecundity prediction in healthy couples. In this study, 135 healthy couples were divided into groups according to their fertility. We demonstrated that the sperm CFTR expression level of healthy males who never impregnated their partners (49 cases, 38.68 ± 2.71%) was significantly lower than that of fertile men (86 cases, 46...
August 1, 2017: Andrologia
https://www.readbyqxmd.com/read/28752591/comparison-and-evaluation-of-capacitation-and-acrosomal-reaction-in-freeze-thawed-human-ejaculated-spermatozoa-treated-with-l-carnitine-and-pentoxifylline
#14
E Aliabadi, S Jahanshahi, T Talaei-Khozani, M Banaei
Cryopreservation is used to preserve the spermatozoa; however, it leads to a reduction in sperm quality. L-carnitine (LC) influences sperm motility and preserves the sperm membrane and DNA integrity. The objectives of this study were to evaluate the protective effects of LC on the membrane integrity of normal human spermatozoa and compare it with pentoxifylline (PT) during cryopreservation. Thirty normal semen samples, prepared by swim-up procedure, were divided into three aliquots: a control without any treatment and two experimental aliquots that were incubated in PT or LC for 30 min...
July 28, 2017: Andrologia
https://www.readbyqxmd.com/read/28751536/defective-sperm-head-decondensation-undermines-the-success-of-icsi-in-the-bovine
#15
Luis Águila, Ricardo Felmer, María Elena Arias, Felipe Navarrete, David Martin-Hidalgo, Hoi Chang Lee, Pablo Visconti, Rafael Fissore
The efficiency of intracytoplasmic sperm injection (ICSI) in the bovine is low compared to other species. It is unknown whether defective oocyte activation and/or sperm head decondensation limit the success of this technique in this species. To elucidate where the main obstacle lies, we used homologous and heterologous ICSI and parthenogenetic activation procedures. We also evaluated whether in vitro maturation negatively impacted the early stages of activation after ICSI. Here we showed that injected bovine sperm are resistant to nuclear decondensation by bovine oocytes and this is only partly overcome by exogenous activation...
September 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28749007/reactive-oxygen-species-as-mediators-of-sperm-capacitation-and-pathological-damage
#16
REVIEW
Robert J Aitken
Oxidative stress plays a major role in the life and death of mammalian spermatozoa. These gametes are professional generators of reactive oxygen species (ROS), which appear to derive from three potential sources: sperm mitochondria, cytosolic L-amino acid oxidases, and plasma membrane Nicotinamide adenine dinucleotide phosphate oxidases. The oxidative stress created via these sources appears to play a significant role in driving the physiological changes associated with sperm capacitation through the stimulation of a cyclic adenosine monophosphate/Protein kinase A phosphorylation cascade, including the activation of Extracellular signal regulated kinase-like proteins, massive up-regulation of tyrosine phosphorylation in the sperm tail, as well as the induction of sterol oxidation...
July 27, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28729465/steroid-hormones-regulate-sperm-oviduct-interactions-in-the-bovine
#17
Julie Lamy, Emilie Corbin, Marie-Claire Blache, Anastasiia S Garanina, Rustem Uzbekov, Pascal Mermillod, Marie Saint-Dizier
After insemination in the cow, a sperm reservoir is formed within the oviducts, allowing the storage then progressive release of spermatozoa toward the ovulated oocyte. In order to investigate the hormonal regulation of these events in vitro, the ovarian steroids 17beta-estradiol (E2) and progesterone (P4) were added at various concentrations to monolayers of bovine oviduct epithelial cells (BOEC) before or during co-incubation with spermatozoa. Main findings demonstrate that: (1) a 18-h pretreatment of BOEC with 100 pg/mL and 100 ng/mL of E2 decreased by 25% the ability of BOEC to bind spermatozoa after 10 min, and for the highest dose of E2, 60 min of co-incubation; (2) P4 at concentrations of 10, 100 and 1000 ng/mL induced the release within 60 min of 32 to 47% of bound spermatozoa from BOEC; this sperm releasing effect was maintained after a 18-h pretreatment of BOEC with 100 pg/mL of E2; (3) E2 in concentrations above 100 pg/mL inhibited the releasing effect of P4 on bound sperm in a dose-dependent manner; (4) spermatozoa bound to then released from BOEC by the action of P4 induced higher cleavage and blastocyst rates after in vitro fertilization than the control group...
July 20, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28710294/steroid-hormone-receptors-and-direct-effects-of-steroid-hormones-on-ram-spermatozoa
#18
Silvia Gimeno-Martos, Marta González-Arto, Adriana Casao, Margarita Gallego, Jose A Cebrian-Perez, Teresa Muino Blanco, Rosaura Perez-Pe
This study was based on the assumption that steroid hormones present in the female genital tract may have a rapid effect on ram spermatozoa by interaction with specific surface receptors. We demonstrate the presence of progesterone (PR) and estrogen (ER) receptors in ram spermatozoa, their localization changes during in vitro capacitation and the actions of progesterone (P4) and 17β-estradiol (E2) on ram sperm functionality. Immunolocalization assays revealed the presence of PR mainly at the equatorial region of ram spermatozoa...
July 14, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28701622/effects-on-glycocalyx-structures-of-frozen-thawed-bovine-sperm-induced-by-flow-cytometry-and-artificial-capacitation
#19
Kohei Umezu, Yuuki Hiradate, Takashi Numabe, Kenshiro Hara, Kentaro Tanemura
Sperm sorting by flow cytometry is a useful technology in the bovine industry, but the conception rates after artificial insemination using sex-sorted sperm are lower than when using the un-sorted sperm. In this study, we have investigated the causes for these low conception rates. We have focused on changes caused by flow cytometry to the glycocalyx, which forms the outermost surface of the sperm membrane. We have also evaluated the effects of capacitation on the glycocalyx since capacitation involves a redistribution of the sperm membrane that is vital for successful fertilization and conception...
July 13, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28667465/the-role-of-vitamin-d-in-male-fertility-a-focus-on-the-testis
#20
REVIEW
Cristina de Angelis, Mariano Galdiero, Claudia Pivonello, Francesco Garifalos, Davide Menafra, Federica Cariati, Ciro Salzano, Giacomo Galdiero, Mariangela Piscopo, Alfonso Vece, Annamaria Colao, Rosario Pivonello
In the last decade, vitamin D has emerged as a pleiotropic molecule with a multitude of autocrine, paracrine and endocrine functions, mediated by classical genomic as well as non-classical non-genomic actions, on multiple target organs and systems. The expression of vitamin D receptor and vitamin D metabolizing enzymes in male reproductive system, particularly in the testis, suggests the occurrence of vitamin D synthesis and regulation as well as function in the testis. The role of vitamin D in the modulation of testis functions, including hormone production and spermatogenesis, has been investigated in animals and humans...
September 2017: Reviews in Endocrine & Metabolic Disorders
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