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sperm capacitation

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https://www.readbyqxmd.com/read/28442049/prion-protein-2-dublet-gene-prnd-role-in-ovine-semen-capacitation-cryopreservation-and-fertility
#1
L M Ferreira, M Garcia-Herreros, A Domingos, C C Marques, P Mesquita, J P Barbas, M C Baptista, J Pimenta, A E M Horta, J A M Prates, R M L N Pereira
The aim of the present study was to examine the role of Doppel protein in the capacitation process and fertilising ability of both fresh and frozen-thawed (FT) spermatozoa from rams carrying different prion protein 2 (dublet) (PRND) gene polymorphisms. The detection efficacy of new anti-Doppel monoclonal antibodies and PRND mRNA quantification were also explored in ovine spermatozoa. Three different genotypes (AA, GA, GG) were identified for codon 26 of ovine PRND-c.78G>A. Using flow cytometry, a higher fluorescence was detected in fresh compared with FT sperm samples incubated with anti-Doppel primary and fluorescein isothiocyanate-conjugated secondary antibodies (P<0...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28432466/calpain-inhibition-prevents-flotillin-re-ordering-and-src-family-activation-during-capacitation
#2
Deneb Maldonado-García, Monica L Salgado-Lucio, Ana L Roa-Espitia, Tania Reyes-Miguel, Enrique O Hernández-González
Prior to fertilization, mammalian sperm undergo several molecular, biochemical and physiological changes in a process termed capacitation. However, the mechanisms explaining the involvement of cytoskeletal remodeling and membrane re-ordering in each process prior to fertilization remain poorly understood. We found that the migration of both flotillin microdomains and Src family kinases towards the apical ridge of guinea pig sperm occurs under capacitating conditions. This re-ordering is associated with spectrin cleavage by calpain...
April 22, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28431864/toward-an-integrative-and-predictive-sperm-quality-analysis-in-bos-taurus
#3
J L Yániz, C Soler, C Alquézar-Baeta, P Santolaria
There is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS(®)3Fun kit, were performed...
March 31, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28419524/-fibronectin-from-oviductal-cells-fluctuates-during-the-estrous-cycle-and-contributes-to-sperm-oviduct-interaction-in-cattle
#4
C E Osycka-Salut, L Castellano, D Fornes, J S Beltrame, Cai Alonso, A Jawerbaum, A Franchi, E S Díaz, S Perez Martinez
During the passage of sperm through the oviduct, spermatozoa bind to the oviductal epithelium and form the oviductal reservoir. This interaction keeps the fertilizing capacity of sperm until ovulation-associated signals induce sperm release from the oviductal epithelium, allowing the transit of spermatozoa to the fertilization site. Fibronectin is a glycoprotein from the extracellular matrix that binds to α5β1 receptors. Fibronectin has been found to be expressed in the oviduct, whereas α5β1 has been found to be expressed in the sperm of different species...
April 17, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28418610/localization-patterns-of-the-ganglioside-gm1-in-human-sperm-are-indicative-of-male-fertility-and-independent-of-traditional-semen-measures
#5
Cristina Cardona, Queenie V Neri, Alana J Simpson, Melissa A Moody, G Charles Ostermeier, Eric K Seaman, Theodore Paniza, Zev Rosenwaks, Gianpiero D Palermo, Alexander J Travis
Semen analysis lacks a functional component and best identifies extreme cases of infertility. The ganglioside GM1 is known to have functional roles during capacitation and acrosome exocytosis. Here, we assessed whether GM1 localization patterns (Cap-Score™) correspond with male fertility in different settings: Study 1 involved couples pursuing assisted reproduction in a tertiary care fertility clinic, while Study 2 involved men with known fertility versus those questioning their fertility at a local urology center...
April 18, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28418600/validation-of-a-laboratory-developed-test-of-human-sperm-capacitation
#6
Melissa A Moody, Cristina Cardona, Alana J Simpson, T Timothy Smith, Alexander J Travis, G Charles Ostermeier
Sperm must undergo capacitation to become fertilization competent. Here we validated that monosialotetrahexosylganglioside (GM1 ) localization patterns, which were assessed in the Cap-Score™ Sperm Function Test, reflect a capacitated state in human sperm. First, we defined patterns representing sperm that do or do not respond to stimuli for capacitation. Sperm with "capacitated" patterns had exposed acrosomal carbohydrates and underwent acrosome exocytosis in response to calcium ionophore (A23187). Precision was evaluated by percent change of the Cap-Score measured for 50, 100, 150, and 200 sperm...
April 18, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28397297/participation-of-phosphofructokinase-malate-dehydrogenase-and-isocitrate-dehydrogenase-in-capacitation-and-acrosome-reaction-of-boar-spermatozoa
#7
E Breininger, D Dubois, V E Pereyra, P C Rodriguez, M M Satorre, P D Cetica
The aim of this work was to determine the enzymatic activity of phosphofructokinase (PFK), malate dehydrogenase (MDH) and isocitrate dehydrogenase (IDH) in boar spermatozoa and study their participation in bicarbonate-induced capacitation and follicular fluid-induced acrosome reaction. Enzymatic activity of these enzymes was determined spectrophotometrically in extracts of boar spermatozoa. Sperm suspensions were incubated in the presence of bicarbonate (40 mM), a well-known capacitation inducer, or follicular fluid (30%), as an acrosome reaction inducer, and different concentrations of oxoglutarate, oxalomalate and hydroxymalonate, inhibitors of PFK, IDH and MDH, respectively...
April 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28395326/signaling-mechanisms-in-mammalian-sperm-motility%C3%A2
#8
Maria João Freitas, Srinivasan Vijayaraghavan, Margarida Fardilha
The goal of sperm is to fertilize the oocyte. To achieve that purpose, it must acquire motility in the epididymis and hyperactivated motility in the female reproductive tract. Motility is only achieved when the sperm presents a fully functional flagellum, is capable of producing energy to fuel the movement, and suffers epididymal maturation and capacitation. Since sperm is a transcriptionally silent cell, motility depends on the activation and/or inhibitions of key signaling pathways. This review describes and discusses the main signaling pathways involved in primary and hyperactivated motility, as well as the bioenergetic mechanisms necessary to produce energy to fuel sperm motility...
January 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28392800/cytotoxic-and-genotoxic-effects-of-acephate-on-human-sperm
#9
M A Thamali Dhanushka, L Dinithi C Peiris
Extensive use of organophosphorus pesticides (OPs) could alter semen quality and sperm DNA at different stages of spermatogenesis. Acephate is a highly toxic extensively used OP and, therefore, we aimed to evaluate the effects of acephate on human semen quality and sperm DNA integrity. Sperm collected from healthy males were exposed to 0, 50, 100, and 200 μg/mL of acephate and incubated for 1 h, 2 h, and 3 h. Subsequently, sperm motility, vitality, functional integrity of plasma membrane, sperm capacitation, and DNA damage were examined...
2017: Journal of Toxicology
https://www.readbyqxmd.com/read/28390187/prediction-of-male-fertility-using-capacitation-associated-proteins-in-spermatozoa
#10
REVIEW
Md Saidur Rahman, Woo-Sung Kwon, Myung-Geol Pang
Infertility and subfertility account for huge economic losses in the animal industry; indeed, 50% of animal breeding failure is associated with male infertility. Approximately 70% of cattle and 90% of pig livestock are currently produced by artificial insemination. Therefore, breeding-male selection is extremely important for the genetic benefits of progeny. Although conventional semen analysis provides an initial measure of male fertility, its clinical value is questionable. Proteomics approaches recently identified candidate protein markers in spermatozoa for evaluating male fertility...
April 8, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28379343/jnk-and-p38-mapk-pathways-link-capacitation-with-apoptosis-and-seminal-plasma-proteins-protect-sperm-by-interfering-with-both-routes
#11
Carolina Luna, Noelia Mendoza, Adriana Casao, Rosaura Pérez-Pé, José A Cebrián-Pérez, Teresa Muiño-Blanco
The Mitogen-activated protein kinase (MAPK) JNK and p38 signaling cascades are involved in triggering apoptosis in somatic cells. Given that spermatozoa are able to undergo apoptosis, we tested the hypothesis that these pathways might be functional in ram spermatozoa as two signal transduction mechanisms that contribute to the modulation of capacitation and apoptosis. Indirect immunofluorescence and western-blot analysis evidenced the presence of JNK and p38 in ram spermatozoa. To verify the involvement of these enzymes in sperm physiology, we determined the effect of specific inhibitors of JNK or p38 on in vitro capacitation induced with either cAMP-elevating agents or Epidermal Growth Factor (EGF)...
March 31, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28377896/leptin-improves-sperm-cryopreservation-via-antioxidant-defense
#12
Paula Fontoura, Mariana Duque Mello, Paulo Gallo-Sá, Maria Cecília Erthal-Martins, Maria Cecília Almeida Cardoso, Cristiane Ramos
BACKGROUND: Leptin and its receptor are present in spermatozoa; however, the role of leptin in sperm function is still controversial. Our present study aimed at demonstrating the effect of cryopreservation on sperm DNA fragmentation (DNAf) and investigating the possible effects of sperm capacitation techniques and leptin in vitro incubation on frozen-thawed sperm DNAf and oxidative stress. METHODS: Samples of 45 normospermic men attending for infertility investigation at Vida Centro de Fertilidade, Rio de Janeiro, Brazil, were frozen and thawed with or without capacitation and leptin incubation prior to freezing...
January 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28377504/a-genetic-variant-of-the-sperm-specific-slo3-k-channel-has-altered-ph-and-ca2-sensitivities
#13
Yanyan Geng, Juan J Ferreira, Victor Dzikunu, Alice Butler, Pascale Lybaert, Peng Yuan, Karl L Magleby, Lawrence Salkoff, Celia M Santi
To fertilize an oocyte, sperm must first undergo capacitation in which the sperm plasma membrane becomes hyperpolarized via activation of potassium (K+) channels and resultant K+ efflux. Sperm-specific SLO3 K+ channels are responsible for these membrane potential changes critical for fertilization in mouse sperm and they are only sensitive to pHi. However, in human sperm, the major K+ conductance is both Ca2+ and pHi-sensitive. It is been debated whether Ca2+-sensitive SLO1 channels substitute for hSLO3 in human sperm, or whether human SLO3 channels have acquired Ca2+ sensitivity...
April 4, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28375100/quercetin-inhibits-human-sperm-functions-by-reducing-sperm-ca2-i-and-tyrosine-phosphorylation
#14
Xiaolei Liang, Zhili Xia, Jiexi Yan, Yiqing Wang, Shilong Xue, Xuehong Zhang
Quercetin is widely known as potent natural antioxidant and scavenger of reactive oxygen species (ROS) and nitric oxide both in vitro and in vivo. Quercetin has a wide range of biological functions and health-promoting effects. There are more and more interests in the addition of this flavonol to various traditional food products. However, the in vitro toxicity of quercetin to mature human sperm remains unknown. In this study, we investigated the in vitro effects of quercetin on human sperm functions. The results showed that the total sperm motility were significantly inhibited compared to the controls following exposure to 100, 200 and 400µM quercetin for 6 and 12h; quercetin did not affect human sperm viability...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28356183/cryopreservation-of-saltwater-crocodile-crocodylus-porosus-spermatozoa
#15
S D Johnston, E Qualischefski, J Cooper, R McLeod, J Lever, B Nixon, A L Anderson, R Hobbs, J Gosálvez, C López-Fernández, T Keeley
The aim of the present study was to develop a protocol for the successful cryopreservation of Saltwater crocodile spermatozoa. Sperm cells were frozen above liquid nitrogen vapour in phosphate-buffered saline (PBS) containing either 0.3M trehalose, 0.3M raffinose or 0.3M sucrose and compared with glycerol (0.3-2.7M). Although the highest levels of mean post-thaw motility were observed following cryopreservation in 0.3M trehalose (7.6%) and 0.3M sucrose (7.3%), plasma membrane integrity (PI) was best following cryopreservation in 2...
March 30, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28344405/biochemical-components-of-seminal-plasma-and-their-correlation-to-the-fresh-seminal-characteristics-in-marwari-stallions-and-poitou-jacks
#16
Thirumala Rao Talluri, Gorakh Mal, Sanjay Kumar Ravi
AIM: To investigate various biochemical components of seminal plasma in Marwari stallions and Poitou Jacks and to find out their correlation with that of the seminal characteristics. MATERIALS AND METHODS: In this study, semen was collected from six Marwari stallions and six Poitou jacks aged from 4 to 6 years and with known fertility status. The semen collection from the stallions were collected during the breeding season, i.e., between the months of April and June...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28339693/neuronal-signaling-repertoire-in-the-mammalian-sperm-functionality
#17
Alfredo Ramírez-Reveco, Franz Villarroel-Espíndola, Joan E Rodríguez-Gil, Ilona I Concha
The common embryonic origin has been a recurrent explanation to understand the presence of "neural receptors" in sperm. However, this designation has conditioned a bias marked by the classical neurotransmission model, dismissing the possibility that neurotransmitters can play specific roles in the sperm function by themselves. For instance, the launching of acrosome reaction, a fundamental sperm function, includes several steps that recall the process of presynaptic secretion. Unlike of postsynaptic neuron, whose activation is mediated by molecular interaction between neurotransmitter and postsynaptic receptors, the oocyte activation is not mediated by receptors, but by cytosolic translocation of sperm phospholipase (PLCζ)...
March 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28297559/molecular-changes-and-signaling-events-occurring-in-spermatozoa-during-epididymal-maturation
#18
REVIEW
M G Gervasi, P E Visconti
After leaving the testis, spermatozoa have not yet acquired the ability to move progressively and are unable to fertilize oocytes. To become fertilization competent, they must go through an epididymal maturation process in the male, and capacitation in the female tract. Epididymal maturation can be defined as those changes occurring to spermatozoa in the epididymis that render the spermatozoa the ability to capacitate in the female tract. As part of this process, sperm cells undergo a series of biochemical and physiological changes that require incorporation of new molecules derived from the epididymal epithelium, as well as post-translational modifications of endogenous proteins synthesized during spermiogenesis in the testis...
March 2017: Andrology
https://www.readbyqxmd.com/read/28280418/intracellular-calcium-dependent-regulation-of-the-sperm-specific-calcium-activated-potassium-channel-hslo3-by-the-bkca-activator-ldd175
#19
Tharaka Darshana Wijerathne, Jihyun Kim, Dongki Yang, Kyu Pil Lee
Plasma membrane hyperpolarization associated with activation of Ca(2+)-activated K(+) channels plays an important role in sperm capacitation during fertilization. Although Slo3 (slowpoke homologue 3), together with the auxiliary γ2-subunit, LRRC52 (leucine-rich-repeat-containing 52), is known to mediate the pH-sensitive, sperm-specific K(+) current KSper in mice, the molecular identity of this channel in human sperm remains controversial. In this study, we tested the classical BKCa activators, NS1619 and LDD175, on human Slo3, heterologously expressed in HEK293 cells together with its functional interacting γ2 subunit, hLRRC52...
March 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28261842/sperm-rna-preparation-for-transcriptomic-analysis-review-of-the-techniques-and-personal-experience
#20
S El Fekih, M-H Nguyen, A Perrin, D Beauvillard, F Morel, A Saad, H Ben Ali, M De Braekeleer
In the last 10 years, several approaches, including microarrays, have been applied to investigate sperm transcript levels. However, success using microarray profiling is highly dependent of the quality of the RNA obtained. Therefore, the development of methods that deliver highly purified and intact RNA is of utmost importance. The three steps used to achieve this goal, purification of spermatozoa, RNA extraction and evaluation of RNA quality, are reviewed. Following that review and preliminary experiments, we processed sperm samples from seven normozoospermic men with a combination of gradient centrifugation and somatic cell lysis...
March 6, 2017: Andrologia
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