keyword
MENU ▼
Read by QxMD icon Read
search

sperm capacitation

keyword
https://www.readbyqxmd.com/read/28077244/the-catsper-channel-modulates-boar-sperm-motility-during-capacitation
#1
Alejandro Vicente-Carrillo, Manuel Álvarez-Rodríguez, Heriberto Rodríguez-Martínez
The cation channel of sperm (CatSper) comprises four transmembrane subunits specifically expressed in human, equine, murine and ovine spermatozoa, apparently implicated in capacitation, hyperactivation and acrosome exocytosis. Western blotting and immunocytochemistry showed hereby that CatSper subunits are also present in boar spermatozoa, primarily over the sperm neck, tail and cytoplasmic droplets; albeit CatSper -1 presented in addition some distribution over the membrane of the acrosome and CatSper -2 and -4 over the membrane of the post-acrosome...
January 8, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28076980/status-of-sperm-born-oocyte-activating-factors-pawp-plc%C3%AE-and-sperm-chromatin-in-uncapacitated-capacitated-and-acrosome-reacted-conditions
#2
Marziyeh Tavalaee, Kazem Parivar, Abdol-Hossein Shahverdi, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani
The aim of this study was to investigate the value of sperm factors PAWP and PLCζ involved in oocyte activation and sperm chromatin status, as indicators of sperm quality during uncapacitated, capacitated and acrosome-reacted conditions. Semen samples collected from 10 normozoospermic men and sperm factors PAWP, PLCζ, protamine deficiency and DNA fragmentation evaluated in fresh samples, processed sample by DGC (density gradient centrifugation), capacitated and acrosome reacted sperm, using immunofluorescence and flow cytometry...
January 12, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28063926/luteinizing-hormone-modulates-intracellular-calcium-protein-tyrosine-phosphorylation-and-motility-during-human-sperm-capacitation
#3
Aideé S López-Torres, María E González-González, Esperanza Mata-Martínez, Fernando Larrea, Claudia L Treviño, Mayel Chirinos
In order to fertilize, spermatozoa must undergo physiological and biochemical changes during their transit along the female reproductive tract before reaching and fusing with the oocyte, process known as capacitation. Sperm modifications associated with capacitation are modulated by their interaction with molecules present in the female reproductive tract. During the woman fertile window, some reproductive hormones reach their maximum concentrations in serum, such as the luteinizing hormone (LH). Since spermatozoa preparing to fertilize may be exposed to LH, the purpose of this work was to study the effects of this hormone on intracellular Ca(2+) concentrations ([Ca(2+)]i), protein tyrosine phosphorylation, sperm motility and acrosome reaction under capacitating conditions...
January 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28054406/surfactant-protein-d-regulates-murine-testicular-immune-milieu-and-sperm-functions
#4
Sushama Rokade, Uday Kishore, Taruna Madan
PROBLEM: Surfactant protein D (SP-D), a pattern recognition protein that regulates inflammation and immune homoeostasis, is expressed by testicular germ cells under the influence of testosterone. This study investigates the role of SP-D in testicular immune privilege and sperm functions. METHOD OF STUDY: Testicular levels of cytokines and immunoregulatory molecules were evaluated in lipopolysaccharide (LPS)-challenged SP-D gene knockout mice (SP-D(-/-) ). Further, sperm functions were assessed by computer-assisted sperm analyser (CASA) and in vitro capacitation...
January 5, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28049872/involvement-of-calcium-channels-and-intracellular-calcium-in-bull-sperm-thermotaxis
#5
Md Anisuzzaman Mondal, Yuji Takagi, Shoji A Baba, Koh-Ichi Hamano
Thermotaxis that sperm migrate to higher temperature area has been confirmed in rabbit and human. In this study, we examined the migration ability of bull sperm in a temperature gradient to confirm thermotaxis and elucidate the involvement of calcium in such thermotaxis, as well as the relation between sperm capacitation and bull fertility. Thermotaxis was evaluated in a temperature gradient of 34-42ºC using a cross-type column 22-mm long, 40-mm wide, and 100-μm deep. Significantly more sperm migrated to the high-temperature area of 39ºC in a 2ºC temperature gradient, and to 40ºC in a 1ºC temperature gradient...
December 31, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28043371/sperm-bound-antisperm-antibodies-prevent-capacitation-of-bovine-spermatozoa
#6
M S Ferrer, J Klabnik-Bradford, D E Anderson, A C Bullington, R A Palomares, L M J Miller, R Stawicki, M Miesner
It was hypothesized here that sperm-bound antisperm antibodies (ASAs) impair the ability of bovine spermatozoa to undergo capacitation, bind to the zona pellucida, and complete the acrosome reaction. The effect of ASA binding on these functions was evaluated in frozen/thawed spermatozoa from four bulls before and after induction of ASAs. Ejaculates were divided into ASA negative (<10% immunoglobulin [Ig]G- and IgA-bound spermatozoa) or ASA positive (≥20% IgG and/or IgA-bound spermatozoa). The percentage of capacitated (Merocyanine 540 positive) live spermatozoa in response to heparin was lower in ASA-positive than ASA-negative ejaculates (P < 0...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28025393/peroxiredoxins-prevent-oxidative-stress-during-human-sperm-capacitation
#7
Donghyun Lee, Adel R Moawad, Tania Morielli, Maria C Fernandez, Cristian O'Flaherty
STUDY QUESTION: Do peroxiredoxins (PRDXs) control reactive oxygen species (ROS) levels during human sperm capacitation? SUMMARY ANSWER: PRDXs are necessary to control the levels of ROS generated during capacitation allowing spermatozoa to achieve fertilizing ability. WHAT IS KNOWN ALREADY: Sperm capacitation is an oxidative event that requires low and controlled amounts of ROS to trigger phosphorylation events. PRDXs are antioxidant enzymes that not only act as scavengers but also control ROS action in somatic cells...
December 26, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28017538/sedimentation-properties-in-density-gradients-correspond-with-levels-of-sperm-dna-fragmentation-chromatin-compaction-and-binding-affinity-to-hyaluronic-acid
#8
Forough Torabi, Adel Binduraihem, David Miller
Mature spermatozoa bind hyaluronic acid in the extracellular matrix via hyaladherins. Immature spermatozoa may be unable to interact because they do not express the appropriate hyaladherins on their surface. Fresh human semen samples were fractionated using differential density gradient centrifugation (DDGC) and the ability of these fractions to bind hyaluronic acid was evaluated. The presence of sperm hyaladherins was also assessed. CD44 was located mainly on the hyaluronic and equatorial segment and became more restricted to the equatorial segment in capacitated spermatozoa...
December 15, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/27992447/heat-sensing-receptor-trpv1-is-a-mediator-of-thermotaxis-in-human-spermatozoa
#9
Luca De Toni, Andrea Garolla, Massimo Menegazzo, Sabina Magagna, Andrea Di Nisio, Iva Šabović, Maria Santa Rocca, Valentina Scattolini, Andrea Filippi, Carlo Foresta
The molecular bases of sperm thermotaxis, the temperature-oriented cell motility, are currently under investigation. Thermal perception relies on a subclass of the transient receptor potential [TRP] channels, whose member TRPV1 is acknowledged as the heat sensing receptor. Here we investigated the involvement of TRPV1 in human sperm thermotaxis. We obtained semen samples from 16 normozoospermic subjects attending an infertility survey programme, testis biopsies from 6 patients with testicular germ cell cancer and testis fine needle aspirates from 6 patients with obstructive azoospermia undergoing assisted reproductive technologies...
2016: PloS One
https://www.readbyqxmd.com/read/27921298/female-induced-remote-regulation-of-sperm-physiology-may-provide-opportunities-for-gamete-level-mate-choice
#10
Jukka Kekäläinen, Jonathan P Evans
In sedentary externally fertilizing species, direct interactions between mating partners are limited and prefertilization communication between sexes occurs largely at the gamete level. Certain combinations of eggs and sperm often have higher fertilization success than others, which may be contingent on egg-derived chemical factors that preferentially attract sperm from compatible males. Here, we examine the mechanisms underlying such effects in the marine mussel Mytilus galloprovincialis, where differential sperm attraction has recently been shown to be associated with variation in offspring viability...
December 6, 2016: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/27912195/mass-spectrometry-profiling-of-oxysterols-in-human-sperm-identifies-25-hydroxycholesterol-as-a-marker-of-sperm-function
#11
Chiara Zerbinati, Luisa Caponecchia, Rosa Puca, Marco Ciacciarelli, Pietro Salacone, Annalisa Sebastianelli, Antonio Pastore, Giovanni Palleschi, Vincenzo Petrozza, Natale Porta, Rocco Rago, Antonio Carbone, Luigi Iuliano
Cholesterol is a main lipid component of sperm cell that is essential for sperm membrane fluidity, capacitation, and acrosomal reaction. Recent data obtained in bovine sperm showed that sperm capacitation is associated to the formation of oxysterols, oxidized products of cholesterol. The aim of this study was to profile oxysterol content in human semen, and to investigate their potential role in sperm pathophysiology. Among the 12 oxysterols analyzed, 25-hydroxycholesterol (25-HC) resulted the most represented in normozoospermic samples, and its concentration positively correlated with spermatozoa number...
November 22, 2016: Redox Biology
https://www.readbyqxmd.com/read/27902451/vitamin-d-and-male-reproductive-system
#12
Pablo R Costanzo, Pablo Knoblovits
Vitamin D deficiency is a highly prevalent worldwide condition and affects people of all ages. The most important role of vitamin D is the regulation of intestinal calcium absorption and metabolism of calcium and phosphorus to maintain muscle and bone homeostasis. Furthermore, in recent years it has been discovered that the vitamin D receptor (VDR) is widely distributed in many organs and tissues where vitamin D can perform other actions that include the modulation of the immune response, insulin secretion, anti-proliferative effect on cells of vascular smooth muscle, modulation of the renin-angiotensin-aldosterone system and regulates cell growth in several organs...
December 1, 2016: Hormone Molecular Biology and Clinical Investigation
https://www.readbyqxmd.com/read/27901058/a-kinase-anchoring-protein-4-akap4-is-an-erk1-2-substrate-and-a-switch-molecule-between-camp-pka-and-pkc-erk1-2-in-human-spermatozoa
#13
Liat Rahamim Ben-Navi, Tal Almog, Zhong Yao, Rony Seger, Zvi Naor
Mammalian spermatozoa undergo capacitation and acrosome reaction in order to fertilize the egg. The PKC-ERK1/2 pathway plays an important role in human spermatozoa motility, capacitation and the acrosome reaction. Here we demonstrate that ERK1/2 phosphorylates proAKAP4 on Thr265 in human spermatozoa in vitro and in vivo. Cyclic AMP (cAMP) had no effect on ERK1/2 activity in human spermatozoa, but stimulated the MAPK in mouse pituitary LβT2 gonadotrope cells. cAMP via PKA attenuates PKC-dependent ERK1/2 activation only in the presence of proAKAP4...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27895910/proteomic-identification-of-cryostress-in-epididymal-spermatozoa
#14
Sung Jae Yoon, Md Saidur Rahman, Woo Sung Kwon, Do Yeal Ryu, Yoo Jin Park, Myung Geol Pang
BACKGROUND: Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. We assessed the motility (%), motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining...
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/27883267/translocation-of-tektin-3-to-the-equatorial-segment-of-heads-in-bull-spermatozoa-exposed-to-dibutyryl-camp-and-calyculin-a
#15
Mariko Tsukamoto, Erina Hiyama, Karen Hirotani, Takafumi Gotoh, Tetsuichiro Inai, Hiroshi Iida
Tektins (TEKTs) are filamentous proteins associated with microtubules in cilia, flagella, basal bodies, and centrioles. Five TEKTs (TEKT1, -2, -3, -4, and -5) have been identified as components of mammalian sperm flagella. We previously reported that TKET1 and -3 are also present in the heads of rodent spermatozoa. The present study clearly demonstrates that TEKT2 is present at the acrosome cap whereas TEKT3 resides just beneath the plasma membrane of the post-acrosomal region of sperm heads in unactivated bull spermatozoa, and builds on the distributional differences of TEKT1, -2, and -3 on sperm heads...
November 23, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27876896/the-reproductive-toxicity-of-cdse-zns-quantum-dots-on-the-in-vivo-ovarian-function-and-in-vitro-fertilization
#16
Gaixia Xu, Guimiao Lin, Suxia Lin, Na Wu, Yueyue Deng, Gang Feng, Qiang Chen, Junle Qu, Danni Chen, Siping Chen, Hanben Niu, Shujiang Mei, Ken-Tye Yong, Xiaomei Wang
Despite the usefulness of quantum dots (QDs) in biomedicine and optoelectronics, their toxicity risks remain a major obstacle for clinical usages. Hence, we studied the reproductive toxicity of CdSe/ZnS QDs on two aspects, (i) in vivo ovarian functions and (ii) in vitro fertilization process. The body weight, estrous cycles, biodistribution of QDs, and oocyte maturation are evaluated on female mice treated with QDs. The mRNA level of the follicle-stimulating hormone receptor (FSHr) and luteinizing hormone receptor (LHr) in ovaries are assayed...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27865407/resveratrol-prevents-capacitation-like-changes-and-improves-in%C3%A2-vitro-fertilizing-capability-of-buffalo-frozen-thawed-sperm
#17
Valentina Longobardi, Gianluigi Zullo, Angela Salzano, Carolina De Canditiis, Andrea Cammarano, Luca De Luise, Maria Valeria Puzio, Gianluca Neglia, Bianca Gasparrini
The aim of this study was to evaluate the effect of resveratrol supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. After the initial semen assessment, buffalo semen was cryopreserved in BioXcell containing 0 (control group), 0.5, 1, 10, and 50-μM resveratrol. After thawing, viability, motility, and capacitation status (assessed by localization of phosphotyrosine-containing proteins) were evaluated. Based on the results of the dose-response trial, the concentration of 50 μM was selected for further assessments, such as membrane integrity, total antioxidant capacity, reactive oxygen species, and lipid peroxidation (LPO) levels...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27864906/role-and-regulation-of-glycogen-synthase-kinase-3-beta-in-bovine-spermatozoa
#18
Michael Belenky, Haim Breitbart
The serine/threonine kinase Glycogen synthase kinase 3 (GSK-3) is a master switch that regulates a multitude of cellular pathways, including the acrosome reaction in sperm. In epididymal sperm cells, for example, GSK-3 activity correlates with inhibition of motility-yet no direct pathways connecting GSK-3 activation with loss of motility have been described. Indeed, the details of how GSK-3 is regulated during sperm capacitation and the acrosome reaction remains obscure. To this end, we addressed the involvement of the GSK-3 beta isoform in several known pathways that contribute to motility and the acrosome reaction...
November 19, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27861489/characterizing-transcriptional-networks-in-male-rainbow-darter-etheostoma-caeruleum-that-regulate-testis-development-over-a-complete-reproductive-cycle
#19
Paulina A Bahamonde, Mark E McMaster, Mark R Servos, Christopher J Martyniuk, Kelly R Munkittrick
Intersex is a condition that has been associated with exposure to sewage effluents in male rainbow darter (Etheostoma caeruleum). To better understand changes in the transcriptome that are associated with intersex, we characterized annual changes in the testis transcriptome in wild, unexposed fish. Rainbow darter males were collected from the Grand River (Ontario, Canada) in May (spawning), August (post-spawning), October (recrudescence), January (developing) and March (pre-spawning). Histology was used to determine the proportion of spermatogenic cell types that were present during each period of testicular maturation...
2016: PloS One
https://www.readbyqxmd.com/read/27860455/papaverine-sensitive-phosphodiesterase-activity-is-measured-in-bovine-spermatozoa
#20
A Bergeron, A Hébert, C Guillemette, A Laroche, M-P Poulin, J P Aragon, P Leclerc, R Sullivan, P Blondin, C Vigneault, F J Richard
Cyclic adenosine monophosphate (cAMP) plays a crucial role as a signaling molecule for capacitation, motility, and acrosome reaction in mammalian spermatozoa. It is well-known that cAMP degradation by phosphodiesterase (PDE) enzyme has a major impact on sperm functions. This study was undertaken to characterize cAMP-PDE activity in bovine spermatozoa. Total cAMP-PDE activity in cauda epididymal and ejaculated spermatozoa was 543.2 ± 49.5 and 1252.6 ± 86.5 fmoles/min/10(6) spermatozoa, respectively. Using different family-specific PDE inhibitors, we showed that in cauda epididymal and ejaculated spermatozoa, the major cAMP-PDE activity was papaverine-sensitive (44...
January 2017: Andrology
keyword
keyword
74886
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"