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sperm capacitation

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https://www.readbyqxmd.com/read/28810916/the-catsper-channel-and-its-roles-in-male-fertility-a-systematic-review
#1
REVIEW
Xiang-Hong Sun, Ying-Ying Zhu, Lin Wang, Hong-Ling Liu, Yong Ling, Zong-Li Li, Li-Bo Sun
The Catsper channel is a sperm-specific, Ca(2+)-permeable, pH-dependent, and low voltage-dependent channel that is essential for the hyperactivity of sperm flagellum, chemotaxis towards the egg, capacitation and acrosome reaction. All of these physiological events require calcium entry into sperm cells. Remarkably, Catsper genes are exclusively expressed in the testis during spermatogenesis, and are sensitive to ion channel-induced pH change, such as NHEs, Ca(2+)ATPase, K(+) channel, Hv1 channel and HCO3(-) transporters...
August 15, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28794880/relationship-between-semenogelins-bound-to-human-sperm-and-other-semen-parameters-and-pregnancy-outcomes
#2
Kazumitsu Yamasaki, Kaoru Yoshida, Miki Yoshiike, Kazuhiko Shimada, Hiroyuki Nishiyama, Satoru Takamizawa, Kaoru Yanagida, Teruaki Iwamoto
BACKGROUND: Semenogelins (SEMGs) are major components of human seminal vesicle secretions. Due to SEMG's sperm-motility inhibitor, a significant negative correlation between sperm motility and the proportion of SEMG-bound spermatozoa (SEMG+) was found in asthenozoospermic patients. SEMGs also show intrinsic inhibitory capability for sperm capacitation; however, studies on actual clinical specimens have not been conducted. METHODS: To reveal the relationship between SEMGs and the fertilizing capacity of sperm from male infertile patients who are not restricted to asthenozoospermia, we measured the proportion of SEMG+ in the spermatozoa of 142 male infertile patients...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28770098/boar-management-and-semen-handling-factors-affect-the-quality-of-boar-extended-semen
#3
REVIEW
Alfonso Lopez Rodriguez, Ann Van Soom, Ioannis Arsenakis, Dominiek Maes
Artificial insemination (AI) is the preferred method for reproduction in the majority of the intensive pig production systems Worldwide. To this end, fresh extended ready-to-use semen doses are either purchased from AI-centres or produced by boars kept on-farm. For profitable semen production, it is necessary to obtain a maximum amount of high quality semen from each boar. This paper reviews current knowledge on factors that may affect semen quality by influencing the boar or the semen during processing. Genetic markers could be used for early detection of boars with the highest fertility potential...
2017: Porcine Health Management
https://www.readbyqxmd.com/read/28762521/sperm-cystic-fibrosis-transmembrane-conductance-regulator-expression-level-is-relevant-to-fecundity-of-healthy-couples
#4
P-B Sun, H-M Xu, K Li, H-C Li, A-J Chen, M-J Chen, H-T Dai, Y Ni
Cystic fibrosis transmembrane conductance regulator (CFTR) is relevant to sperm quality, sperm capacitation and male fertility. However, it is still unknown whether CFTR can be a potential parameter for fecundity prediction in healthy couples. In this study, 135 healthy couples were divided into groups according to their fertility. We demonstrated that the sperm CFTR expression level of healthy males who never impregnated their partners (49 cases, 38.68 ± 2.71%) was significantly lower than that of fertile men (86 cases, 46...
August 1, 2017: Andrologia
https://www.readbyqxmd.com/read/28752591/comparison-and-evaluation-of-capacitation-and-acrosomal-reaction-in-freeze-thawed-human-ejaculated-spermatozoa-treated-with-l-carnitine-and-pentoxifylline
#5
E Aliabadi, S Jahanshahi, T Talaei-Khozani, M Banaei
Cryopreservation is used to preserve the spermatozoa; however, it leads to a reduction in sperm quality. L-carnitine (LC) influences sperm motility and preserves the sperm membrane and DNA integrity. The objectives of this study were to evaluate the protective effects of LC on the membrane integrity of normal human spermatozoa and compare it with pentoxifylline (PT) during cryopreservation. Thirty normal semen samples, prepared by swim-up procedure, were divided into three aliquots: a control without any treatment and two experimental aliquots that were incubated in PT or LC for 30 min...
July 28, 2017: Andrologia
https://www.readbyqxmd.com/read/28751536/defective-sperm-head-decondensation-undermines-the-success-of-icsi-in-the-bovine
#6
Luis Águila, Ricardo Felmer, María Elena Arias, Felipe Navarrete, David Martin-Hidalgo, Hoi Chang Lee, Pablo Visconti, Rafael Fissore
The efficiency of intracytoplasmic sperm injection (ICSI) in the bovine is low compared to other species. It is unknown whether defective oocyte activation and/or sperm head decondensation limit the success of this technique in this species. To elucidate where the main obstacle lies, we used homologous and heterologous ICSI and parthenogenetic activation procedures. We also evaluated whether in vitro maturation negatively impacted the early stages of activation after ICSI. Here we showed that injected bovine sperm are resistant to nuclear decondensation by bovine oocytes and this is only partly overcome by exogenous activation...
September 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28749007/reactive-oxygen-species-as-mediators-of-sperm-capacitation-and-pathological-damage
#7
REVIEW
R J Aitken
Oxidative stress plays a major role in the life and death of mammalian spermatozoa. These gametes are professional generators of reactive oxygen species (ROS), which appear to derive from three potential sources: sperm mitochondria, cytosolic L-amino acid oxidases, and plasma membrane nicotinamide adenine dinucleotide phosphate oxidases. The oxidative stress created via these sources appears to play a significant role in driving the physiological changes associated with sperm capacitation through the stimulation of a cyclic adenosine monophosphate/Protein kinase A phosphorylation cascade, including the activation of Extracellular signal regulated kinase-like proteins, massive up-regulation of tyrosine phosphorylation in the sperm tail, as well as the induction of sterol oxidation...
July 27, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28729465/steroid-hormones-regulate-sperm-oviduct-interactions-in-the-bovine
#8
Julie Lamy, Emilie Corbin, Marie-Claire Blache, Anastasiia S Garanina, Rustem Uzbekov, Pascal Mermillod, Marie Saint-Dizier
After insemination in the cow, a sperm reservoir is formed within the oviducts, allowing the storage then progressive release of spermatozoa toward the ovulated oocyte. In order to investigate the hormonal regulation of these events in vitro, the ovarian steroids 17beta-estradiol (E2) and progesterone (P4) were added at various concentrations to monolayers of bovine oviduct epithelial cells (BOEC) before or during co-incubation with spermatozoa. Main findings demonstrate that: (1) a 18-h pretreatment of BOEC with 100 pg/mL and 100 ng/mL of E2 decreased by 25% the ability of BOEC to bind spermatozoa after 10 min, and for the highest dose of E2, 60 min of co-incubation; (2) P4 at concentrations of 10, 100 and 1000 ng/mL induced the release within 60 min of 32 to 47% of bound spermatozoa from BOEC; this sperm releasing effect was maintained after a 18-h pretreatment of BOEC with 100 pg/mL of E2; (3) E2 in concentrations above 100 pg/mL inhibited the releasing effect of P4 on bound sperm in a dose-dependent manner; (4) spermatozoa bound to then released from BOEC by the action of P4 induced higher cleavage and blastocyst rates after in vitro fertilization than the control group...
July 20, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28710294/steroid-hormone-receptors-and-direct-effects-of-steroid-hormones-on-ram-spermatozoa
#9
Silvia Gimeno-Martos, Marta González-Arto, Adriana Casao, Margarita Gallego, Jose A Cebrian-Perez, Teresa Muino Blanco, Rosaura Perez-Pe
This study was based on the assumption that steroid hormones present in the female genital tract may have a rapid effect on ram spermatozoa by interaction with specific surface receptors. We demonstrate the presence of progesterone (PR) and estrogen (ER) receptors in ram spermatozoa, their localization changes during in vitro capacitation and the actions of progesterone (P4) and 17β-estradiol (E2) on ram sperm functionality. Immunolocalization assays revealed the presence of PR mainly at the equatorial region of ram spermatozoa...
July 14, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28701622/effects-on-glycocalyx-structures-of-frozen-thawed-bovine-sperm-induced-by-flow-cytometry-and-artificial-capacitation
#10
Kohei Umezu, Yuuki Hiradate, Takashi Numabe, Kenshiro Hara, Kentaro Tanemura
Sperm sorting by flow cytometry is a useful technology in the bovine industry, but the conception rates after artificial insemination using sex-sorted sperm are lower than when using the un-sorted sperm. In this study, we have investigated the causes for these low conception rates. We have focused on changes caused by flow cytometry to the glycocalyx, which forms the outermost surface of the sperm membrane. We have also evaluated the effects of capacitation on the glycocalyx since capacitation involves a redistribution of the sperm membrane that is vital for successful fertilization and conception...
July 13, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28667465/the-role-of-vitamin-d-in-male-fertility-a-focus-on-the-testis
#11
REVIEW
Cristina de Angelis, Mariano Galdiero, Claudia Pivonello, Francesco Garifalos, Davide Menafra, Federica Cariati, Ciro Salzano, Giacomo Galdiero, Mariangela Piscopo, Alfonso Vece, Annamaria Colao, Rosario Pivonello
In the last decade, vitamin D has emerged as a pleiotropic molecule with a multitude of autocrine, paracrine and endocrine functions, mediated by classical genomic as well as non-classical non-genomic actions, on multiple target organs and systems. The expression of vitamin D receptor and vitamin D metabolizing enzymes in male reproductive system, particularly in the testis, suggests the occurrence of vitamin D synthesis and regulation as well as function in the testis. The role of vitamin D in the modulation of testis functions, including hormone production and spermatogenesis, has been investigated in animals and humans...
September 2017: Reviews in Endocrine & Metabolic Disorders
https://www.readbyqxmd.com/read/28638922/-palmitoylation-of-heat-shock-protein-90-in-mouse-sperm
#12
Rui Li, Kun Li, Yue Yang, Pei-Bei Sun, Ai-Jun Chen, Ya Ni
Protein palmitoylation, one of post-translation modifications, refers to the addition of saturated 16-carbon palmitic acid to cysteine residues via the thioester bond. It plays key roles in various functional activities, such as the interaction, stability and location of proteins. Heat shock protein 90 (Hsp90), an important molecular chaperone, has been reported to be involved in sperm capacitation. However, it remains unclear whether protein palmitoylation exists in sperm and whether Hsp90 in sperm is palmitoylated under different physiological conditions...
June 25, 2017: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/28597551/testis-specific-isoform-of-na-k-atpase-atp1a4-regulates-sperm-function-and-fertility-in-dairy-bulls-through-potential-mechanisms-involving-reactive-oxygen-species-calcium-and-actin-polymerization
#13
G D Rajamanickam, T Kroetsch, J P Kastelic, J C Thundathil
Traditional bull breeding soundness evaluation (BBSE) eliminates bulls that are grossly abnormal; however, bulls classified as satisfactory potential breeders still vary in field fertility, implying submicroscopic differences in sperm characteristics. The testis-specific isoform of Na/K-ATPase (ATP1A4) is involved in regulation of sperm motility and capacitation in bulls through well-established enzyme activity and signaling functions. The objective was to determine ATP1A4 content, activity and their relationship to post-thaw sperm function and field fertility, using semen samples from low-fertility (LF) and high-fertility (HF) Holstein bulls (n = 20 each) with known FERTSOL rates (measure of field fertility, based on non-return rate)...
June 8, 2017: Andrology
https://www.readbyqxmd.com/read/28585678/human-sperm-motility-capacitation-and-acrosome-reaction-are-impaired-by-2-arachidonoylglycerol-endocannabinoid
#14
M M Francou, J L Girlea, A De Juan, J Ten, R Bernabeu, J De Juan
The endocannabinoids are cannabinoids synthesized by mammalian tissues. These compounds are closely related to the regulation of the male reproductive system. However, little is known about the effects produced by 2-arachidonoylglycerol (2AG) on in vitro human sperm functions. This study was undertaken to determine the effects produced by 2AG on fresh human sperm and in the capacitation technique. Semen samples from healthy young men were exposed to different concentrations of 2AG before and during capacitation technique...
June 6, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/28559132/effect-of-reduced-glutathione-supplementation-in-semen-extender-on-tyrosine-phosphorylation-and-apoptosis-like-changes-in-frozen-thawed-hariana-bull-spermatozoa
#15
Nadeem Shah, Vijay Singh, Hanuman Prasad Yadav, Meena Verma, Dharmendra Singh Chauhan, Atul Saxena, Sarvajeet Yadav, Dilip Kumar Swain
To provide new insights into the mechanisms through which reduced glutathione (GSH) is able to protect spermatozoa, we tested the hypothesis that cryocapacitation and apoptosis like changes can contribute to the negative effect of freezing and thawing on bull spermatozoa, and that GSH prevent this damage. Having known protective effects of GSH in terms of a potent antioxidant, we evaluated capacitation, tyrosine phosphorylation and apoptosis like changes in bull spermatozoa after freezing and thawing in egg yolk tris glycerol extender containing (0...
May 20, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28543743/detection-and-localization-of-regucalcin-in-spermatozoa-of-water-buffalo-bubalus-bubalis-a-calcium-regulating-multifunctional-protein
#16
H Pillai, A M Shende, M S Parmar, J Thomas, H S Kartha, G Taru Sharma, S K Ghosh, S K Bhure
Regucalcin (RGN) is a calcium-regulating, anti-apoptotic, antioxidative and antiproliferative multifunctional protein predominantly seen in liver and kidney. All these functions are very crucial during spermatogenesis and sperm maturation process until fertilization of the ovum. Although many studies have reported the wide distribution of regucalcin in the male reproductive tract of the rat, human and bovine, its presence in spermatozoa is yet to be demonstrated wherein calcium has a pivotal role in the transport, capacitation, acrosomal reaction and further fusion with ova...
May 24, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28521061/extracellular-camp-activates-molecular-signalling-pathways-associated-with-sperm-capacitation-in-bovines
#17
Carlos Agustín I Alonso, Claudia E Osycka-Salut, Luciana Castellano, Andreína Cesari, Nicolás Di Siervi, Adrián Mutto, Anders Johannisson, Jane M Morrell, Carlos Davio, Silvina Perez-Martinez
STUDY QUESTION: Is extracellular cAMP involved in the regulation of signalling pathways in bovine sperm capacitation? SUMMARY ANSWER: Extracellular cAMP induces sperm capacitation through the activation of different signalling pathways that involve phospholipase C (PLC), PKC/ERK1-2 signalling and an increase in sperm Ca2+ levels, as well as soluble AC and cAMP/protein kinase A (PKA) signalling. WHAT IS KNOWN ALREADY: In order to fertilize the oocyte, ejaculated spermatozoa must undergo a series of changes in the female reproductive tract, known as capacitation...
August 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28513911/sperm-capacitation-pretreatment-positively-impacts-bovine-intracytoplasmic-sperm-injection
#18
Luis Águila, Fabiola Zambrano, Maria E Arias, Ricardo Felmer
The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is low compared to other species due in part to inadequate egg activation and sperm nucleus decondensation after injection. We hypothesized that this low efficiency is due to the lack of complete sperm capacitation, so we evaluated the effects of isobutylmethylxanthine (IBMX) and methyl-β-cyclodextrin (MβCD) on bovine sperm capacitation and on the preimplantation developmental potential of bovine embryos generated by ICSI. Treatment with IBMX and MβCD decreased sperm viability (between 13-30%); nevertheless, 0...
July 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28507119/regulation-of-the-sperm-calcium-channel-catsper-by-endogenous-steroids-and-plant-triterpenoids
#19
Nadja Mannowetz, Melissa R Miller, Polina V Lishko
The calcium channel of sperm (CatSper) is essential for sperm hyperactivated motility and fertility. The steroid hormone progesterone activates CatSper of human sperm via binding to the serine hydrolase ABHD2. However, steroid specificity of ABHD2 has not been evaluated. Here, we explored whether steroid hormones to which human spermatozoa are exposed in the male and female genital tract influence CatSper activation via modulation of ABHD2. The results show that testosterone, estrogen, and hydrocortisone did not alter basal CatSper currents, whereas the neurosteroid pregnenolone sulfate exerted similar effects as progesterone, likely binding to the same site...
May 30, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28488738/comparison-of-three-different-extenders-on-murrah-buffaloes-bubalus-bubalis-semen-freezability
#20
M F Zorzetto, I Martin, Y F R Sancler-Silva, S Zoca, C P Freitas-Dell'Aqua, F O Papa, A A Ramos, J F Nunes, C C M Salgueiro, E Oba
The use of frozen semen for artificial insemination is the main approach utilised for the genetic improvement of most domesticated species. The advantages include lower transportation costs, continuous availability of semen, fewer occurrences of sexually transmitted diseases and the incorporation of desirable genes in a relatively short amount of time. Nevertheless, the use of frozen semen in buffalo herds remains limited due to the loss of sperm quality when buffalo semen is frozen. So, the goal of this study was to evaluate the pre- and post-cryopreservation quality of buffalo semen diluted in three distinct freezing media: Tris-egg yolk, Botu-bov® (BB) and ACP-111®...
May 10, 2017: Andrologia
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