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sperm capacitation

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https://www.readbyqxmd.com/read/29782044/effect-of-various-levels-of-dissolved-oxygen-on-reactive-oxygen-species-and-cryocapacitation-like-changes-in-bull-sperm
#1
Beigh Y Amin, Jai K Prasad, Subrata K Ghosh, Shabir A Lone, Abhishek Kumar, Abdul R Mustapha, Omer Din, Ajay Kumar
The present investigation was carried out to study the effect of various levels of dissolved oxygen (DO) on reactive oxygen species (ROS) and cryocapacitation-like changes in bull sperm. Egg yolk-Tris-glycerol (EYTG) extender was split into four subextenders; viz., Extender I (control; no flushing with liquid nitrogen (LN2 )), Extender II, Extender III and Extender IV were flushed with LN2 for 40, 16 and 8 min, respectively. The DO levels were standardized to 11.7, 2, 4 and 8 ppm, respectively, in control (Extender I), Extender II, Extender III and Extender IV...
May 21, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29778923/quantitative-proteomic-profiling-indicates-the-difference-in-reproductive-efficiency-between-meishan-and-duroc-boar-spermatozoa
#2
Li Xinhong, Linqing Zhen, Jieli Fu, Lirui Wang, Qiangzhen Yang, Peifei Li, Yuhua Li
The reproductive efficiency of Meishan pigs is higher than that of Duroc pigs, but the underlying molecular mechanism for this disparity remains unclear. No systematic quantitative proteomics studies, comparing global proteins in Meishan and Duroc boar spermatozoa have been reported. Therefore, we applied iTRAQ labeling coupled with mass spectrometry, and analyzed the differences in proteins between Meishan and Duroc sperm. In the present study, a total of 1597 proteins were quantified. Of these proteins, 190 showed statistically significant fold changes between Meishan and Duroc spermatozoa...
April 30, 2018: Theriogenology
https://www.readbyqxmd.com/read/29750435/signaling-pathways-involved-in-human-sperm-hyperactivated-motility-stimulated-by-zn-2
#3
Deborah Allouche-Fitoussi, Danit Bakhshi, Haim Breitbart
To fertilize the egg, sperm cells must reside in the female reproductive tract for several hours during which they undergo chemical and motility changes collectively called capacitation. During capacitation, the sperm develop a unique type of motility known as hyper-activated motility (HAM). The semen contains Zn2+ in millimolar concentrations, whereas in the female reproductive tract the concentration is around 1 µM. In this study, we characterize the role of Zn2+ in human sperm capacitation focusing on its effect on HAM...
May 11, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29747159/seminal-plasma-influences-the-fertilizing-potential-of-cryopreserved-stallion-sperm
#4
Essraa M Al-Essawe, Margareta Wallgren, Manuela Wulf, Christine Aurich, Beatriz Macías-García, Ylva Sjunnesson, Jane M Morrell
Seminal plasma (SP) contains proteins that may influence cryosurvival and prevent capacitation-like changes due to freezing and thawing. The objective of this study was to investigate the effect of adding pooled SP from "good" (GF) or "bad" (BF) freezer stallions on sperm cells' fertilizing ability. "Good freezers" refers to stallions that usually produce ejaculates which can withstand cryopreservation, whilst "bad freezer" stallions produce ejaculates which cannot tolerate the freezing process...
April 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29743243/cftr-enac-dependent-regulation-of-membrane-potential-during-human-sperm-capacitation-is-initiated-by-bicarbonate-uptake-through-nbc
#5
Lis C Puga Molina, Nicolas A Pinto, Nicolás I Torres, Ana L Gonzalez-Cota, Guillermina M Luque, Paula A Balestrini, Ana Romarowski, Dario Krapf, Celia M Santi, Claudia L Trevino, Alberto Darszon, Mariano G Buffone
To fertilize an egg, sperm must reside in the female reproductive tract to undergo several maturational changes that are collectively referred to as capacitation. From a molecular point of view, the HCO3--dependent activation of the atypical soluble adenylyl cyclase (ADCY10) is one of the first events that occurs during capacitation and leads to the subsequent cAMP-dependent activation of protein kinase A (PKA). Capacitation is also accompanied by hyperpolarization of the sperm plasma membrane. We previously reported that PKA activation is necessary for CFTR (Cystic Fibrosis Transmembrane Conductance Regulator Channel) activity and for the modulation of membrane potential (Em)...
May 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29733332/ccdc87-is-critical-for-sperm-function-and-male-fertility
#6
Tongtong Wang, Qianqian Yin, Xuehao Ma, Ming-Han Tong, Yuchuan Zhou
Male infertility has become an increasingly common health concern in recent years. Apart from environmental factors, nutrition, lifestyle, and sexually transmitted diseases, genetic defects are important causes of male infertility. Many genes have been demonstrated to be associated with male infertility. However, the roles of some functional genes in infertility, especially those that are specifically expressed in the reproductive system, remain to be elucidated. Here, we demonstrated that the testis-specific gene coiled-coil domain-containing 87 (Ccdc87) is critical for male fertility...
May 3, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29729147/assessment-of-azadirachtin-a-a-neem-tetranortritarpinoid-on-rat-spermatozoa-during-in-vitro-capacitation
#7
Teesta V Katte, Malempati Rajyalakshmi, Ravindranath H Aladakatti
BACKGROUND: The exploration of the biological assessment of technical azadirachtin, a tetranortritarpinoid from the neem seed kernel, was reviewed. The present study was, therefore, designed to evaluate the dose-dependent in vitro effects of azadirachtin-A, particularly on the functional studies and determination of molecular events, which are critical in the process of sperm capacitation. METHODS: To assess the effects of the azadirachtin-A on the functional studies, sperm capacitation, the total sperm adenosine triphosphate levels, acrosome reaction (AR), the sperm-egg interaction and the determination of molecular events like cyclic adenosine-3',5'-monophosphate and calcium levels, the appropriate volumes of the sperm suspension were added to the medium to a final concentration of 1×106 sperm/mL and incubated in a humidified atmosphere of 5% CO2 in air at 37°C...
May 5, 2018: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/29723838/alterations-in-oxidative-responses-and-post-translational-modification-caused-by-p-p%C3%A2-dde-in-mus-spretus-testes-reveal-cys-oxidation-status-in-proteins-related-to-cell-redox-homeostasis-and-male-fertility
#8
José Alhama, Carlos A Fuentes-Almagro, Nieves Abril, Carmen Michán
The major derivate of DDT, 1,1-dichloro-2,2-bis (p-chlorophenyl) ethylene (p,p´-DDE), is a persistent pollutant previously associated with oxidative stress. Additionally, p,p´-DDE has been linked to several metabolic alterations related to sexual function in rodents. In this study, we analysed the effects of a non-lethal p,p´-DDE dose to Mus spretus mice in testes, focusing on oxidative damage to biomolecules, defence mechanisms against oxidative stress and post-translational protein modifications. No increase in lipid or DNA oxidation was observed, although antioxidative enzymatic defences and redox status of glutathione were altered in several ways...
April 30, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29717688/review-semen-handling-time-of-insemination-and-insemination-technique-in-cattle
#9
M G Diskin
In cattle artificial insemination plays not only a vital role in the successful establishment of pregnancy, which is a prerequisite for initiation of the subsequent lactation, but also in accelerating genetic improvement and facilitating the distribution of semen from genetically elite sires. The latter has been greatly facilitated by the ability to successfully cryopreserve semen. The objective of an insemination is to ensure that there is an adequate reservoir of competent, capacitated, motile sperm in the caudal region of the oviductal isthmus, the site of the main sperm reservoir in the cow, at the time of ovulation to ensure fertilisation...
May 2, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29700114/disruption-of-protein-kinase-a-localization-induces-acrosomal-exocytosis-in-capacitated-mouse-sperm
#10
Cintia Stival, Carla Ritagliati, Xinran Xu, Maria G Gervasi, Guillermina M Luque, Carolina Baro Graf, José Luis Vega-Beltran, Nicolás I Torres, Alberto Darszon, Diego Krapf, Mariano G Buffone, Pablo Visconti, Dario Krapf
Protein kinase A (PKA) is a broad-spectrum Ser/Thr kinase involved in the regulation of several cellular activities. Thus, its precise activation relies on being localized at specific subcellular places known as discrete PKA signalosomes. A-Kinase Anchoring Proteins (AKAPs) form scaffolding assemblies that play a pivotal role on PKA regulation by restricting its activity to specific micro-domains. Since one of the first signaling events observed during mammalian sperm capacitation is PKA activation, understanding how PKA activity is restricted in space and time is crucial to decipher the critical steps of sperm capacitation...
April 26, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29694849/regulation-mechanisms-and-implications-of-sperm-membrane-hyperpolarization
#11
REVIEW
Carla Ritagliati, Carolina Baró Graf, Cintia Stival, Dario Krapf
Mammalian sperm are unable to fertilize the egg immediately after ejaculation. In order to gain fertilization competence, they need to undergo a series of biochemical and physiological modifications inside the female reproductive tract, known as capacitation. Capacitation correlates with two essential events for fertilization: hyperactivation, an asymmetric and vigorous flagellar motility, and the ability to undergo the acrosome reaction. At a molecular level, capacitation is associated to: phosphorylation cascades, modification of membrane lipids, alkalinization of the intracellular pH, increase in the intracellular Ca2+ concentration and hyperpolarization of the sperm plasma membrane potential...
April 22, 2018: Mechanisms of Development
https://www.readbyqxmd.com/read/29691903/relation-between-respiratory-activity-and-sperm-parameters-in-boar-spermatozoa-cryopreserved-with-alpha-tocopherol-and-selected-by-sephadex
#12
M M Satorre, E Breininger, P D Cetica, M Córdoba
Our aim was to evaluate the effect of Sephadex filtration on respiratory activity of porcine spermatozoa and its relation with quality and functional sperm parameters. Samples were evaluated regarding oxygen uptake and sperm parameters: motility, plasma and acrosome membrane integrity, capacitation and acrosome reaction induction in vitro, plasma membrane functionality, determined by the hypo-osmotic swelling test (HOST), and lipid peroxidation assessed by thiobarbituric acid assay. Sephadex filtration improved all routine quality parameters (motility, plasma and acrosome membrane integrity) and functional parameters (HOST, in vitro capacitation and true acrosome reaction levels) and produced a significant decrease in cryocapacitation and lipid peroxidation...
April 24, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29667544/proteomic-markers-in-cervical-mucus
#13
Yolanda Fernandez-Hermida, Giuseppe Grande, Micaela Menarguez, Anna Laura Astorri, Rafael Azagra
Cervical Mucus (CM) is a viscous fluid produced by the cervical glands. It plays a key role in the transport and capacitation of sperm through the female genital tract. The CM undergoes modifications that make it have different biophysical and biochemical characteristics throughout the cycle, becoming an important element for the identification of ovulation. Since CM is rich in secreted proteins, it may represent moreover a source of putative biomarkers for female reproductive tract diseases. This review is an attempt to collect relevant knowledge about the physicochemical properties and functions of the cervical mucus, including its important role as a clinical marker of female fertility, and draws attention to CM as a source of potential proteomic biomarkers...
April 18, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29660628/transcriptional-abundance-of-type-1-endocannabinoid-receptor-cb1-and-fatty-acid-amide-hydrolase-faah-in-bull-spermatozoa-relationship-with-field-fertility
#14
Vimlesh Kumar, Arumugam Kumaresan, Pradeep Nag, Puneeth Kumar, Tirtha Kumar Datta, Rubina Kumari Baithalu, Tushar Kumar Mohanty
A highly sophisticated endogenous cannabinoid system (ECS) has been shown to play a crucial role in controlling sperm functions and fertility in men. In the present study, we report the differences in the expression level of components of ECS [type-1 endocannabinoid receptor (CB1) and fatty acid amide hydrolase (FAAH)] in spermatozoa from bulls with different field fertility ratings. Cryopreserved spermatozoa from crossbred cattle bulls (n = 40) were utilized for the study. The bulls were classified into high-, medium- and low-fertile bulls based on field conception rates...
April 8, 2018: Theriogenology
https://www.readbyqxmd.com/read/29659949/emc10-governs-male-fertility-via-maintaining-sperm-ion-balance
#15
Yuchuan Zhou, Fei Wu, Man Zhang, Zuquan Xiong, Qianqian Yin, Yanfei Ru, Huijuan Shi, Jinsong Li, Shanhua Mao, Yanliang Li, Xinyi Cao, Renming Hu, Chong Wee Liew, Qiang Ding, Xuanchun Wang, Yonglian Zhang
Infertility is a severe public health problem worldwide that prevails up to 15% in reproductive-age couples, and male infertility accounts for half of total infertility. Studies on genetically modified animal models have identified lots of genes involved in the pathogenesis of male infertility. The underlying causes, however, remain largely unclear. In this study, we provide evidence that EMC10, one subunit of endoplasmic reticulum (ER) membrane protein complex (EMC), is required for male fertility. EMC10 is significantly decreased in spermatozoa from patients with asthenozoospermia and positively associated with human sperm motility...
April 6, 2018: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/29656213/molecular-characterization-of-voltage-gated-potassium-channel-kv-and-its-importance-in-functional-dynamics-in-bull-spermatozoa
#16
Rishi Kumar Gupta, Dilip Kumar Swain, Vijay Singh, Mukul Anand, Soumen Choudhury, Sarvajeet Yadav, Atul Saxena, Satish Kumar Garg
Present study was undertaken to characterize the voltage gated potassium channel (Kv 1.1) in bull spermatozoa using sixty four ejaculates collected from four Hariana bulls. Functional characterization was undertaken using a selective blocker of Kv channel, 4-Aminopyridine (4-AP) while molecular presence of Kv on bull spermatozoa by immunoblotting and indirect immunofluorescence. Three sets of 100 μL diluted sperm samples namely-negative control (100 μL of sperm dilution medium (SDM) containing 10 × 106  cells), vehicle control (99 μL of SDM containing 10 × 106  cells, and DMSO- 1  μL) and 4-AP treatment group (99 μL of SDM containing 10 × 106  cells, and drug 1 μL 4-AP) were used in the study...
March 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29642908/putative-human-sperm-interactome-a-networks-study
#17
Alessandra Ordinelli, Nicola Bernabò, Massimiliano Orsini, Mauro Mattioli, Barbara Barboni
BACKGROUND: For over sixty years, it has been known that mammalian spermatozoa immediately after ejaculation are virtually infertile. They became able to fertilize only after they reside for long time (hours to days) within female genital tract where they complete their functional maturation, the capacitation. This process is finely regulated by the interaction with the female environment and involves, in spermatozoa, a myriad of molecules as messengers and target of signals. Since, to date, a model able to represent the molecular interaction that characterize sperm physiology does not exist, we realized the Human Sperm Interactme Network3...
April 11, 2018: BMC Systems Biology
https://www.readbyqxmd.com/read/29626105/murine-sperm-capacitation-oocyte-penetration-and-decondensation-following-moderate-alcohol-intake
#18
Melisa Sanchez, Vanina Andrea Fontana, Camila Galotto, Maite Cambiasso, Cristian Sobarzo, Lucrecia Calvo, Juan Carlos Calvo, Elisa Cebral
Male chronic alcohol abuse causes testicular failure and infertility. We analyzed the effects of moderate sub-chronic alcohol intake on sperm morphology, capacitation, fertilization and sperm head decondensation. CF-1 male mice were administered 15% ethanol in drinking water for 15 days; control mice received ethanol-free water. Similar patterns of tyrosine phosphorylation were observed in capacitated spermatozoa of control and treated males. Percentage of hyperactivation (H) and spontaneous (SAR) and progesterone-induced (IAR) acrosome reaction significantly decreased at 120 and 150 minutes of capacitation in treated males compared to controls (H: 14...
April 6, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29618635/removal-of-sialic-acid-from-bull-sperm-decreases-motility-and-mucus-penetration-ability-but-increases-zona-pellucida-binding-and-polyspermic-penetration-in-vitro
#19
Beatriz Fernandez-Fuertes, Alfonso Blanco-Fernandez, Colm J Reid, Kieran Meade, S Fair, Pat Lonergan
This study tested the hypothesis that sperm sialic acid (Sia) is required to reach the site of fertilization, and that successful fertilization requires recognition of Sia from both the sperm and oocyte to occur. In addition, it has recently been reported that Siglecs (Sia-binding-immunoglobulin-like lectins) are present on the sperm surface. Thus, the possibility that the recognition of oocyte Sia was sperm-Siglec-mediated was also addressed. Sperm exposed to neuraminidase (NMase) exhibited lower overall and progressive motility, which translated to a decreased ability to swim through cervical mucus from cows in oestrus...
April 4, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29605167/the-sperm-mitochondrion-organelle-of-many-functions
#20
REVIEW
Christa R Moraes, Stuart Meyers
This review summarizes current research in sperm mitochondrial function with specific emphasis on mitochondrial metabolism, reactive oxygen species production and mitochondrial genomics. This organelle is key in many crucial sperm functions including motility, hyperactivation, capacitation, acrosome reaction, and fertilization, thus its role in male fertility cannot be ignored. Recent studies have further elucidated sperm metabolism, placing greater emphasis on the importance of mitochondrial energy production for some species...
March 26, 2018: Animal Reproduction Science
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