keyword
MENU ▼
Read by QxMD icon Read
search

sperm capacitation

keyword
https://www.readbyqxmd.com/read/27921298/female-induced-remote-regulation-of-sperm-physiology-may-provide-opportunities-for-gamete-level-mate-choice
#1
Jukka Kekäläinen, Jonathan P Evans
In sedentary externally fertilizing species, direct interactions between mating partners are limited and pre-fertilization communication between sexes occur largely at the gamete level. Certain combinations of eggs and sperm often have higher fertilization success than others, which may be contingent on egg-derived chemical factors that preferentially attract sperm from compatible males. Here, we examine the mechanisms underlying such effects in the marine mussel Mytilus galloprovincialis, where differential sperm attraction has recently been shown to be associated with variation in offspring viability...
December 6, 2016: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/27912195/mass-spectrometry-profiling-of-oxysterols-in-human-sperm-identifies-25-hydroxycholesterol-as-a-marker-of-sperm-function
#2
Chiara Zerbinati, Luisa Caponecchia, Rosa Puca, Marco Ciacciarelli, Pietro Salacone, Annalisa Sebastianelli, Antonio Pastore, Giovanni Palleschi, Vincenzo Petrozza, Natale Porta, Rocco Rago, Antonio Carbone, Luigi Iuliano
Cholesterol is a main lipid component of sperm cell that is essential for sperm membrane fluidity, capacitation, and acrosomal reaction. Recent data obtained in bovine sperm showed that sperm capacitation is associated to the formation of oxysterols, oxidized products of cholesterol. The aim of this study was to profile oxysterol content in human semen, and to investigate their potential role in sperm pathophysiology. Among the 12 oxysterols analyzed, 25-hydroxycholesterol (25-HC) resulted the most represented in normozoospermic samples, and its concentration positively correlated with spermatozoa number...
November 22, 2016: Redox Biology
https://www.readbyqxmd.com/read/27902451/vitamin-d-and-male-reproductive-system
#3
Pablo R Costanzo, Pablo Knoblovits
Vitamin D deficiency is a highly prevalent worldwide condition and affects people of all ages. The most important role of vitamin D is the regulation of intestinal calcium absorption and metabolism of calcium and phosphorus to maintain muscle and bone homeostasis. Furthermore, in recent years it has been discovered that the vitamin D receptor (VDR) is widely distributed in many organs and tissues where vitamin D can perform other actions that include the modulation of the immune response, insulin secretion, anti-proliferative effect on cells of vascular smooth muscle, modulation of the renin-angiotensin-aldosterone system and regulates cell growth in several organs...
December 1, 2016: Hormone Molecular Biology and Clinical Investigation
https://www.readbyqxmd.com/read/27901058/a-kinase-anchoring-protein-4-akap4-is-an-erk1-2-substrate-and-a-switch-molecule-between-camp-pka-and-pkc-erk1-2-in-human-spermatozoa
#4
Liat Rahamim Ben-Navi, Tal Almog, Zhong Yao, Rony Seger, Zvi Naor
Mammalian spermatozoa undergo capacitation and acrosome reaction in order to fertilize the egg. The PKC-ERK1/2 pathway plays an important role in human spermatozoa motility, capacitation and the acrosome reaction. Here we demonstrate that ERK1/2 phosphorylates proAKAP4 on Thr265 in human spermatozoa in vitro and in vivo. Cyclic AMP (cAMP) had no effect on ERK1/2 activity in human spermatozoa, but stimulated the MAPK in mouse pituitary LβT2 gonadotrope cells. cAMP via PKA attenuates PKC-dependent ERK1/2 activation only in the presence of proAKAP4...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27895910/proteomic-identification-of-cryostress-in-epididymal-spermatozoa
#5
Sung Jae Yoon, Md Saidur Rahman, Woo Sung Kwon, Do Yeal Ryu, Yoo Jin Park, Myung Geol Pang
BACKGROUND: Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. We assessed the motility (%), motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining...
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/27883267/translocation-of-tektin-3-to-the-equatorial-segment-of-heads-in-bull-spermatozoa-exposed-to-dibutyryl-camp-and-calyculin-a
#6
Mariko Tsukamoto, Erina Hiyama, Karen Hirotani, Takafumi Gotoh, Tetsuichiro Inai, Hiroshi Iida
Tektins (TEKTs) are filamentous proteins associated with microtubules in cilia, flagella, basal bodies, and centrioles. Five TEKTs (TEKT1, -2, -3, -4, and -5) have been identified as components of mammalian sperm flagella. We previously reported that TKET1 and TEKT3 are also present in the heads of rodent spermatozoa. The present study clearly demonstrates that TEKT2 is present at the acrosome cap whereas TEKT3 resides just beneath the plasma membrane of the post-acrosomal region of sperm heads in unactivated bull spermatozoa, and builds on the distributional differences of TEKT1, -2, and -3 on sperm heads...
November 23, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27876896/the-reproductive-toxicity-of-cdse-zns-quantum-dots-on-the-in-vivo-ovarian-function-and-in-vitro-fertilization
#7
Gaixia Xu, Guimiao Lin, Suxia Lin, Na Wu, Yueyue Deng, Gang Feng, Qiang Chen, Junle Qu, Danni Chen, Siping Chen, Hanben Niu, Shujiang Mei, Ken-Tye Yong, Xiaomei Wang
Despite the usefulness of quantum dots (QDs) in biomedicine and optoelectronics, their toxicity risks remain a major obstacle for clinical usages. Hence, we studied the reproductive toxicity of CdSe/ZnS QDs on two aspects, (i) in vivo ovarian functions and (ii) in vitro fertilization process. The body weight, estrous cycles, biodistribution of QDs, and oocyte maturation are evaluated on female mice treated with QDs. The mRNA level of the follicle-stimulating hormone receptor (FSHr) and luteinizing hormone receptor (LHr) in ovaries are assayed...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27865407/resveratrol-prevents-capacitation-like-changes-and-improves-in%C3%A2-vitro-fertilizing-capability-of-buffalo-frozen-thawed-sperm
#8
Valentina Longobardi, Gianluigi Zullo, Angela Salzano, Carolina De Canditiis, Andrea Cammarano, Luca De Luise, Maria Valeria Puzio, Gianluca Neglia, Bianca Gasparrini
The aim of this study was to evaluate the effect of resveratrol supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. After the initial semen assessment, buffalo semen was cryopreserved in BioXcell containing 0 (control group), 0.5, 1, 10, and 50-μM resveratrol. After thawing, viability, motility, and capacitation status (assessed by localization of phosphotyrosine-containing proteins) were evaluated. Based on the results of the dose-response trial, the concentration of 50 μM was selected for further assessments, such as membrane integrity, total antioxidant capacity, reactive oxygen species, and lipid peroxidation (LPO) levels...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27864906/role-and-regulation-of-glycogen-synthase-kinase-3-beta-in-bovine-spermatozoa
#9
Michael Belenky, Haim Breitbart
The serine/threonine kinase Glycogen synthase kinase 3 (GSK-3) is a master switch that regulates a multitude of cellular pathways, including the acrosome reaction in sperm. In epididymal sperm cells, for example, GSK-3 activity correlates with inhibition of motility-yet no direct pathways connecting GSK-3 activation with loss of motility have been described. Indeed, the details of how GSK-3 is regulated during sperm capacitation and the acrosome reaction remains obscure. To this end, we addressed the involvement of the GSK-3 beta isoform in several known pathways that contribute to motility and the acrosome reaction...
November 19, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27861489/characterizing-transcriptional-networks-in-male-rainbow-darter-etheostoma-caeruleum-that-regulate-testis-development-over-a-complete-reproductive-cycle
#10
Paulina A Bahamonde, Mark E McMaster, Mark R Servos, Christopher J Martyniuk, Kelly R Munkittrick
Intersex is a condition that has been associated with exposure to sewage effluents in male rainbow darter (Etheostoma caeruleum). To better understand changes in the transcriptome that are associated with intersex, we characterized annual changes in the testis transcriptome in wild, unexposed fish. Rainbow darter males were collected from the Grand River (Ontario, Canada) in May (spawning), August (post-spawning), October (recrudescence), January (developing) and March (pre-spawning). Histology was used to determine the proportion of spermatogenic cell types that were present during each period of testicular maturation...
2016: PloS One
https://www.readbyqxmd.com/read/27860455/papaverine-sensitive-phosphodiesterase-activity-is-measured-in-bovine-spermatozoa
#11
A Bergeron, A Hébert, C Guillemette, A Laroche, M-P Poulin, J P Aragon, P Leclerc, R Sullivan, P Blondin, C Vigneault, F J Richard
Cyclic adenosine monophosphate (cAMP) plays a crucial role as a signaling molecule for capacitation, motility, and acrosome reaction in mammalian spermatozoa. It is well-known that cAMP degradation by phosphodiesterase (PDE) enzyme has a major impact on sperm functions. This study was undertaken to characterize cAMP-PDE activity in bovine spermatozoa. Total cAMP-PDE activity in cauda epididymal and ejaculated spermatozoa was 543.2 ± 49.5 and 1252.6 ± 86.5 fmoles/min/10(6) spermatozoa, respectively. Using different family-specific PDE inhibitors, we showed that in cauda epididymal and ejaculated spermatozoa, the major cAMP-PDE activity was papaverine-sensitive (44...
November 16, 2016: Andrology
https://www.readbyqxmd.com/read/27852807/mechanical-tuning-of-mammalian-sperm-behaviour-by-hyperactivation-rheology-and-substrate-adhesion-a-numerical-exploration
#12
Kenta Ishimoto, Eamonn A Gaffney
The great mammalian sperm race encounters numerous microenvironments to which sperm must adapt and a fundamental sperm response is the change in its waveform owing to both fluid rheology and capacitation, with the latter associated with a hyperactivated beat pattern. Hence, in this modelling study, we examine the effect of different flagellar waveforms for sperm behaviour near adhesive substrates, which are representative of epithelia in female tract sperm reservoirs and the zona pellucida (ZP), which surrounds the mammalian egg...
November 2016: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/27836756/cyclic-nucleotide-phosphodiesterases-in-human-spermatozoa-and-seminal-fluid-presence-of-an-active-pde10a-in-human-spermatozoa
#13
Loïze Maréchal, Christine Guillemette, Serge Goupil, Patrick Blondin, Pierre Leclerc, François J Richard
BACKGROUND: Cyclic adenosine monophosphate (cAMP) plays a crucial role as a signaling molecule for sperm functions such as capacitation, motility and acrosome reaction. It is well known that cAMP degradation by phosphodiesterase (PDE) enzyme has a major impact on sperm functions. The present study was undertaken to characterize cAMP-PDE activity in human semen. METHODS: cAMP-PDE activity was measured in human sperm and seminal plasma using family specific PDE inhibitors...
November 9, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27836535/effect-of-the-herbicide-atrazine-and-its-metabolite-dact-on-bovine-sperm-quality
#14
Alisa Komsky-Elbaz, Zvi Roth
Atrazine (ATZ), one of the most extensively used herbicides, is considered a ubiquitous environmental contaminant. ATZ is a known endocrine disruptor, and deleterious effects on reproductive function have been shown, even at low, ecologically relevant doses (0.1-3μg/L). Once it enters the body, ATZ is metabolized to various metabolites, which are further detected in the urine, serum and tissues. In mammals, the major ATZ metabolite is diaminochlorotriazine (DACT). The current study focuses on direct effects of low doses of ATZ and DACT on bovine sperm isolated from ejaculates or epididymis compartments (head, body and tail)...
November 9, 2016: Reproductive Toxicology
https://www.readbyqxmd.com/read/27807702/content-of-testis-specific-isoform-of-na-k-atpase-atp1a4-is-increased-during-bovine-sperm-capacitation-through-translation-in-mitochondrial-ribosomes
#15
Gayathri D Rajamanickam, John P Kastelic, Jacob C Thundathil
Capacitation comprises a series of structural and functional modifications of sperm that confer fertilizing ability. We previously reported that the testis-specific isoform of Na/K-ATPase (ATP1A4) regulated bovine sperm capacitation through signaling mechanisms involving kinases. During subsequent investigations to elucidate mechanisms by which ATP1A4 regulates sperm capacitation, we observed that ATP1A4 was localised in both raft and non-raft fractions of the sperm plasma membrane and that its total content was increased in both membrane fractions during capacitation...
November 2, 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27793453/cholesterol-loaded-cyclodextrins-prevent-cryocapacitation-damages-in-buffalo-bubalus-bubalis-cryopreserved-sperm
#16
Valentina Longobardi, Giuseppe Albero, Carolina De Canditiis, Angela Salzano, Antonio Natale, Anna Balestrieri, Gianluca Neglia, Giuseppe Campanile, Bianca Gasparrini
The aim of this study was to investigate the effect of cholesterol-loaded cyclodextrins (CLC) on motility, viability, capacitation status, and in vivo fertility of buffalo frozen-thawed sperm. After the initial semen assessment, buffalo sperm were diluted in BULLXcell extender containing 0- (control), 1.5-, and 3-mg/mL CLC and cryopreserved. At thawing, sperm motility was evaluated by phase contrast microscopy, and viability-capacitation status was assessed by Hoechst 33258-chlortetracycline (CTC) assay. Capacitation status was also evaluated by an indirect immunofluorescence assay to localize phosphotyrosine-containing proteins...
October 3, 2016: Theriogenology
https://www.readbyqxmd.com/read/27769574/carnitine-supplementation-decreases-capacitation-like-changes-of-frozen-thawed-buffalo-spermatozoa
#17
Valentina Longobardi, Angela Salzano, Giuseppe Campanile, Raffaele Marrone, Francesco Palumbo, Milena Vitiello, Gianluigi Zullo, Bianca Gasparrini
The aim of this study was to evaluate the effect of carnitine supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. Buffalo semen was cryopreserved in BioXcell containing 0 (control group), 2.5 and 7.5-mM carnitine. After thawing, viability, motility, membrane integrity and capacitation status (assessed by localization of phosphotyrosine-containing proteins and chlortetracycline, chlortetracycline assay) were evaluated. Furthermore, total antioxidant capacity, reactive oxygen species (ROS) and lipid peroxidation levels, as well as adenosine triphosphate (ATP) content and phospholipids concentration were assessed...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27714810/essential-role-of-cftr-in-pka-dependent-phosphorylation-alkalinization-and-hyperpolarization-during-human-sperm-capacitation
#18
Lis C Puga Molina, Nicolás A Pinto, Paulina Torres Rodríguez, Ana Romarowski, Alberto Vicens Sanchez, Pablo E Visconti, Alberto Darszon, Claudia L Treviño, Mariano G Buffone
Mammalian sperm require to spend a limited period of time in the female reproductive tract to become competent to fertilize in a process called capacitation. It is well established that HCO3(-) is essential for capacitation because it activates the atypical soluble adenylate cyclase ADCY10 leading to cAMP production, and promotes alkalinization of cytoplasm and membrane hyperpolarization. However, how HCO3(-) is transported into the sperm is not well understood. There is evidence that CFTR activity is involved in the human sperm capacitation but how this channel is integrated in the complex signaling cascades associated with this process remains largely unknown...
October 7, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27707713/cauda-epididymis-specific-beta-defensin-126-promotes-sperm-motility-but-not-fertilizing-ability-in-cattle
#19
Beatriz Fernandez-Fuertes, Fernando Narciandi, Cliona O'Farrelly, Alan K Kelly, Sean Fair, Kieran G Meade, Patrick Lonergan
Bovine beta-defensin 126 (BBD126) exhibits preferential expression for the cauda epididymis of males, where it is absorbed onto the tail and postacrosomal region of the sperm. The aim of this study was to examine the role of BBD126 in bull sperm function. Fresh and frozen-thawed semen were incubated in the presence of different capacitating agents as well as with phosphatidylinositol-specific phospholipase C. These treatments, which have been successful in releasing beta-defensin 126 from macaque sperm, proved to be ineffective in bull sperm...
December 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27707521/use-of-heterospermic-inseminations-and-paternity-testing-to-evaluate-the-relative-contributions-of-common-sperm-traits-and-seminal-plasma-proteins-in-boar-fertility
#20
W L Flowers, F Deller, K R Stewart
The objective of this study was to evaluate relationships between common semen quality estimates including sperm motility, sperm morphology, spontaneous capacitation status and seminal plasma proteins and boar fertility using heterospermic inseminations and subsequent paternity testing. All boars (n=12) used in the study had excellent semen quality (≥70% normal sperm) that resulted in average farrowing rates and litter sizes of 88.9±0.7% and 11.7±0.1 pigs, respectively. Their ejaculates were combined to make heterospermic insemination doses in such a way that each boar was tested against all of his contemporaries...
September 29, 2016: Animal Reproduction Science
keyword
keyword
74886
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"