keyword
MENU ▼
Read by QxMD icon Read
search

sperm capacitation

keyword
https://www.readbyqxmd.com/read/28339693/neuronal-signaling-repertoire-in-the-mammalian-sperm-functionality
#1
Alfredo Ramírez-Reveco, Franz Villarroel-Espíndola, Joan E Rodríguez-Gil, Ilona I Concha
The common embryonic origin has been a recurrent explanation to understand the presence of "neural receptors" in sperm. However, this designation has conditioned a bias marked by the classical neurotransmission model, dismissing the possibility that neurotransmitters can play specific roles in the sperm function by themselves. For instance, the launching of acrosome reaction, a fundamental sperm function, includes several steps that recall the process of presynaptic secretion. Unlike of postsynaptic neuron, whose activation is mediated by molecular interaction between neurotransmitter and postsynaptic receptors, the oocyte activation is not mediated by receptors, but by cytosolic translocation of sperm phospholipase (PLCζ)...
February 20, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28297559/molecular-changes-and-signaling-events-occurring-in-spermatozoa-during-epididymal-maturation
#2
REVIEW
M G Gervasi, P E Visconti
After leaving the testis, spermatozoa have not yet acquired the ability to move progressively and are unable to fertilize oocytes. To become fertilization competent, they must go through an epididymal maturation process in the male, and capacitation in the female tract. Epididymal maturation can be defined as those changes occurring to spermatozoa in the epididymis that render the spermatozoa the ability to capacitate in the female tract. As part of this process, sperm cells undergo a series of biochemical and physiological changes that require incorporation of new molecules derived from the epididymal epithelium, as well as post-translational modifications of endogenous proteins synthesized during spermiogenesis in the testis...
March 2017: Andrology
https://www.readbyqxmd.com/read/28280418/intracellular-calcium-dependent-regulation-of-the-sperm-specific-calcium-activated-potassium-channel-hslo3-by-the-bkca-activator-ldd175
#3
Tharaka Darshana Wijerathne, Jihyun Kim, Dongki Yang, Kyu Pil Lee
Plasma membrane hyperpolarization associated with activation of Ca(2+)-activated K(+) channels plays an important role in sperm capacitation during fertilization. Although Slo3 (slowpoke homologue 3), together with the auxiliary γ2-subunit, LRRC52 (leucine-rich-repeat-containing 52), is known to mediate the pH-sensitive, sperm-specific K(+) current KSper in mice, the molecular identity of this channel in human sperm remains controversial. In this study, we tested the classical BKCa activators, NS1619 and LDD175, on human Slo3, heterologously expressed in HEK293 cells together with its functional interacting γ2 subunit, hLRRC52...
March 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28261842/sperm-rna-preparation-for-transcriptomic-analysis-review-of-the-techniques-and-personal-experience
#4
S El Fekih, M-H Nguyen, A Perrin, D Beauvillard, F Morel, A Saad, H Ben Ali, M De Braekeleer
In the last 10 years, several approaches, including microarrays, have been applied to investigate sperm transcript levels. However, success using microarray profiling is highly dependent of the quality of the RNA obtained. Therefore, the development of methods that deliver highly purified and intact RNA is of utmost importance. The three steps used to achieve this goal, purification of spermatozoa, RNA extraction and evaluation of RNA quality, are reviewed. Following that review and preliminary experiments, we processed sperm samples from seven normozoospermic men with a combination of gradient centrifugation and somatic cell lysis...
March 6, 2017: Andrologia
https://www.readbyqxmd.com/read/28250239/src-family-kinases-in-hamster-spermatozoa-evidence-for-the-presence-of-lck
#5
Durgesh Kumar Singh, Rohit Kumar Deshmukh, Praveen Kumar Narayanan, Shivaji Sisinthy, Archana Bharadwaj Siva
Sperm capacitation is a prerequisite for successful fertilization. Increase in tyrosine phosphorylation is considered the hallmark of capacitation and attempts to understand its regulation are ongoing. In this regard, we attempted to study the role of SRC family kinases (SFKs) in the hamster sperm functions. Interestingly, we found the presence of the lymphocyte-specific protein tyrosine kinase, LCK in mammalian spermatozoa and further characterized it in terms of its localization and function. LCK was found in spermatozoa of several species and its transcript was identified in the hamster testis...
March 1, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28247940/plasma-membrane-calcium-atpase-4-pmca4-co-ordinates-calcium-and-nitric-oxide-signaling-in-regulating-murine-sperm-functional-activity
#6
Kristine E Olli, Kun Li, Deni S Galileo, Patricia A Martin-DeLeon
Reduced sperm motility (asthenospermia) and resulting infertility arise from deletion of the Plasma Membrane Ca(2+) -ATPase 4 (Pmca4) gene which encodes the highly conserved Ca(2+) efflux pump, PMCA4. This is the major Ca(2+) clearance protein in murine sperm. Since the mechanism underlying asthenospermia in PMCA4's absence or reduced activity is unknown, we investigated if sperm PMCA4 negatively regulates nitric oxide synthases (NOSs) and when absent NO, peroxynitrite, and oxidative stress levels are increased...
March 1, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28244620/testis-specific-isoform-of-angiotensin-converting-enzyme-tace-is-involved-in-the-regulation-of-bovine-sperm-capacitation
#7
Mina Ojaghi, John Kastelic, Jacob Thundathil
We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine testes and sperm; and (iii) determine the role of tACE in capacitation. A 110-kDa protein, consistent with the mass of tACE, was detected in sperm extract by our anti-tACE immunoserum. This immunotarget localized at the acrosomal region and principal piece, but was only expressed in testis of mature bulls...
February 28, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28238840/study-of-the-effect-of-ulipristal-acetate-on-human-sperm-ability-to-interact-with-tubal-tissue-and-cumulus-oocyte-complexes
#8
Carlos Zumoffen, Matías D Gómez-Elías, Adriana M Caille, Luis Bahamondes, Patricia S Cuasnicú, Débora J Cohen, María José Munuce
OBJECTIVE: Ulispristal acetate (UPA) is a selective progesterone receptor modulator widely used for emergency contraception (EC). The described main mechanism of action is by inhibiting or delaying ovulation; however, the postovulatory effects of the drug are still on debate. Therefore, the aim of this study was to determine whether UPA could interfere with human sperm fertilizing ability. STUDY DESIGN: Human motile spermatozoa were incubated under capacitating conditions with or without UPA, and then used to inseminate human tubal explants, mouse cumulus-oocyte complexes and zona-free hamster eggs...
February 24, 2017: Contraception
https://www.readbyqxmd.com/read/28238530/a-membrane-associated-adenylate-cyclase-modulates-lactate-dehydrogenase-and-creatine-kinase-activities-required-for-bull-sperm-capacitation-induced-by-hyaluronic-acid
#9
Silvina Fernández, Mariana Córdoba
Hyaluronic acid, as well as heparin, is a glycosaminoglycan present in the female genital tract of cattle. The aim of this study was to evaluate oxidative metabolism and intracellular signals mediated by a membrane-associated adenylate cyclase (mAC), in sperm capacitation with hyaluronic acid and heparin, in cryopreserved bull sperm. The mAC inhibitor, 2',5'-dideoxyadenosine, was used in the present study. Lactate dehydrogenase (LDH) and creatine kinase (CK) activities and lactate concentration were determined spectrophotometrically in the incubation medium...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28238445/pentoxifylline-effects-on-capacitation-and-fertility-of-stallion-epididymal-sperm
#10
P N Guasti, G A Monteiro, R R D Maziero, M T Carmo, J A Dell'Aqua, A M Crespilho, E A Rifai, F O Papa
The aims of this study were to determinate whether pentoxifylline (PTX) increases the motion parameters of fresh and frozen-thawed equine epididymal spermatozoa, to evaluate the tyrosine phosphorylation of frozen-thawed epididymal sperm in the presence of PTX and to determine whether the PTX-treatment of stallion epididymal sperm prior to freezing improves the fertility response of mares to a reduced number of spermatozoa per insemination dose. Fifty epididymis were flushed with a skim milk based extender with or without PTX...
April 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28237350/cryopreservation-of-bull-semen-is-associated-with-carbonylation-of-sperm-proteins
#11
Agnieszka Mostek, Mariola Aleksandra Dietrich, Mariola Słowińska, Andrzej Ciereszko
Artificial insemination with cryopreserved semen enables affordable, large-scale dissemination of gametes with superior genetics. However, cryopreservation can cause functional and structural damage to spermatozoa that is associated with reactive oxygen species (ROS) production, impairment of sperm motility and decreased fertilizing potential, but little attention has been paid to protein changes. The goal of this study was to investigate the oxidative modifications (measured as carbonylation level changes) of bull spermatozoa proteins triggered by the cryopreservation process...
April 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28236106/progesterone-requires-heat-shock-protein-90-hsp90-in-human-sperm-to-regulate-motility-and-acrosome-reaction
#12
Vrushali Sagare-Patil, Rashmi Bhilawadikar, Mosami Galvankar, Kusum Zaveri, Indira Hinduja, Deepak Modi
PURPOSE: The aims of this paper were to study whether heat shock protein 90 (HSP90) is a regulator of sperm functions and to determine its association with oligoasthenozoospermia. METHODS: The levels of HSP90 in sperm lysates were measured by ELISA. Localization of HSP90 and its isoforms was evaluated by immunofluorescence. Sperm motility and kinetics were assessed by computer-assisted sperm analysis. Acrosome reaction was determined by lectin staining. RESULTS: The levels of HSP90 were lower in oligoasthenozoospermic men and correlated positively with the number of motile spermatozoa...
February 24, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28217948/shrna-mediated-ablation-of-prostate-and-testis-expressed-pate-messenger-rna-results-in-impaired-sperm-function-and-fertility
#13
A Rajesh, S Yenugu
Spermatogenesis and sperm maturation are complex processes mediated by a variety of proteins present in the testicular and epididymal mileu. In the recent years, functional characterization of these proteins is being studied by genetic manipulations that involve targeted over expression or knock down of specific genes. In this study, we adopted FuGENE 6-based in vivo transfection of rat cauda epididymis with pGFP-V-RS plasmid that encodes shRNA to knock down Pate mRNA levels to implicate a possible role for this gene in sperm function...
February 19, 2017: Andrology
https://www.readbyqxmd.com/read/28194463/improved-bovine-embryo-production-in-an-oviduct-on-a-chip-system-prevention-of-poly-spermic-fertilization-and-parthenogenic-activation
#14
Marcia A M M Ferraz, Heiko H W Henning, Pedro F Costa, Jos Malda, Ferry P Melchels, R Wubbolts, Tom A E Stout, Peter L A M Vos, Bart M Gadella
The oviduct provides the natural micro-environment for gamete interaction, fertilization and early embryo development in mammals, such as the cow. In conventional culture systems, bovine oviduct epithelial cells (BOEC) undergo a rapid loss of essential differentiated cell properties; we aimed to develop a more physiological in vitro oviduct culture system capable of supporting fertilization. U-shaped chambers were produced using stereo-lithography and mounted with polycarbonate membranes, which were used as culture inserts for primary BOECs...
February 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28185602/impaired-hypothalamic-pituitary-testicular-axis-activity-spermatogenesis-and-sperm-function-promote-infertility-in-males-with-lead-poisoning
#15
Jason Gandhi, Rafael J Hernandez, Andrew Chen, Noel L Smith, Yefim R Sheynkin, Gargi Joshi, Sardar Ali Khan
Lead poisoning is a stealthy threat to human physiological systems as chronic exposure can remain asymptomatic for long periods of time before symptoms manifest. We presently review the biophysical mechanisms of lead poisoning that contribute to male infertility. Environmental and occupational exposure of lead may adversely affect the hypothalamic-pituitary-testicular axis, impairing the induction of spermatogenesis. Dysfunction at the reproductive axis, namely testosterone suppression, is most susceptible and irreversible during pubertal development...
February 10, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28176444/an-intact-acrosome-is-required-for-the-chemotactic-response-to-progesterone-in-mouse-spermatozoa
#16
H A Guidobaldi, N Hirohashi, M Cubilla, M G Buffone, L C Giojalas
Mammalian sperm become fertilization-competent in the oviduct, during a process known as capacitation that involves the acquisition of the ability to exocytose the acrosome but also the chemotactic responses - both of which contribute to successful fertilization. Chemotaxis is used by spermatozoa to orient and to locate the egg; the acrosome reaction facilitates sperm binding to and fusing with the egg membrane. Mammalian spermatozoa are able to sense picomolar concentrations of progesterone, which drives chemotactic behavior...
February 8, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28167483/quercetin-inhibits-human-sperm-functions-by-reducing-sperm-ca2-i-and-tyrosine-phosphorylation
#17
Xiaolei Liang, Zhili Xia, Jiexi Yan, Yiqing Wang, Shilong Xue, Xuehong Zhang
Quercetin is widely known as potent natural antioxidant and scavenger of reactive oxygen species (ROS) and nitric oxide both in vitro and in vivo. Quercetin has a wide range of biological functions and health-promoting effects. There are more and more interests in the addition of this flavonol to various traditional food products. However, the in vitro toxicity of quercetin to mature human sperm remains unknown. In this study, we investigated the in vitro effects of quercetin on human sperm functions. The results showed that the total sperm motility were significantly inhibited compared to the controls following exposure to 100, 200 and 400µM quercetin for 6 and 12h; quercetin did not affect human sperm viability...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28166971/functional-and-molecular-characterization-of-voltage-gated-sodium-channel-nav-1-8-in-bull-spermatozoa
#18
Dharmendra Singh Chauhan, Dilip Kumar Swain, Nadeem Shah, Hanuman Prasad Yadav, Udayraj P Nakade, Vijay Kumar Singh, Rajesh Nigam, Sarvajeet Yadav, Satish Kumar Garg
The aim of our study was to characterize the voltage gated sodium channel Nav 1.8 in bull spermatozoa. Forty ejaculates were collected from four Hariana bulls and semen samples were pooled in view of the nonsignificant variations between different ejaculates. Functional characterization was undertaken using A-803467, a selective blocker of Nav1.8, and veratridine as an opener of the voltage gated sodium channels while molecular characterization was done using western blotting and indirect immunofluorescence assays...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28120399/the-effect-of-cooling-to-different-subzero-temperatures-on-dog-sperm-cryosurvival
#19
A Alcantar-Rodriguez, A Medrano
The objective was to assess the effect of cooling to different subzero temperatures around ice formation (-5°C) on dog sperm cryosurvival and plasma membrane fluidity. Semen was centrifuged, and sperm were resuspended in a Tris-egg yolk medium (3% glycerol). Diluted sperm were cooled from 22 to 5°C, and then, a Tris-egg yolk medium containing 7% glycerol was added (final concentration of 5% glycerol and 200 × 10(6)  cells/ml). Sperm were packaged in 0.5-ml plastic straws, and equilibration was done 16 hr at 5°C before freezing...
January 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28115407/biological-basis-for-human-capacitation-revisited
#20
REVIEW
Christopher De Jonge
BACKGROUND: A little more than a decade ago a review entitled 'Biological basis for human capacitation' was published. A primary conclusion of the review was that with all the technological advances that have been made since the first experiments demonstrated the in vivo requirement of capacitation for fertilization, very little progress had since been made, most significantly for human. OBJECTIVE AND RATIONALE: The present review was carried out to provide an update on the biological basis for human capacitation...
January 23, 2017: Human Reproduction Update
keyword
keyword
74886
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"