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https://www.readbyqxmd.com/read/28636018/fluorescence-lifetime-microscopy-reveals-the-biologically-related-photophysical-heterogeneity-of-oxyblepharismin-in-light-adapted-blue-blepharisma-japonicum-cells
#1
G Checcucci, B Storti, F Ghetti, G Signore, R Bizzarri
The step-up photophobic response of the heterotrich ciliate Blepharisma japonicum is mediated by a hypericinic pigment, blepharismin, which is not present in any of the known six families of photoreceptors, namely rhodopsins, phytochromes, xanthopsins, cryptochromes, phototropins, and BLUF proteins. Upon irradiation, native cells become light-adapted (blue) by converting blepharismin into the photochemically stable oxyblepharismin (OxyBP). So far, OxyBP has been investigated mainly from a photophysical point of view in vitro, either alone or complexed with proteins...
June 21, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28630332/evolutionary-strata-on-young-mating-type-chromosomes-despite-the-lack-of-sexual-antagonism
#2
Sara Branco, Hélène Badouin, Ricardo C Rodríguez de la Vega, Jérôme Gouzy, Fantin Carpentier, Gabriela Aguileta, Sophie Siguenza, Jean-Tristan Brandenburg, Marco A Coelho, Michael E Hood, Tatiana Giraud
Sex chromosomes can display successive steps of recombination suppression known as "evolutionary strata," which are thought to result from the successive linkage of sexually antagonistic genes to sex-determining genes. However, there is little evidence to support this explanation. Here we investigate whether evolutionary strata can evolve without sexual antagonism using fungi that display suppressed recombination extending beyond loci determining mating compatibility despite lack of male/female roles associated with their mating types...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628670/combining-ability-heritability-and-genotypic-relations-of-different-physiological-traits-in-cacao-hybrids
#3
Allan Silva Pereira, Alex-Alan Furtado de Almeida, Márcia Christina da Silva Branco, Marcio Gilberto Cardoso Costa, Dario Ahnert
Selecting parents and evaluating progenies is a very important step in breeding programs and involves approaches such as understanding the initial stages of growth and characterizing the variability among genotypes for different parameters, such as physiological, growth, biomass partitioning and nutrient translocation to the aerial part. In these cases, facilitating tools can be used to understand the involved gene dynamics, such as diallel crosses and genetic and phenotypic correlations. Our main hypothesis is that the contrasting phenotypes of these parental genotypes of cocoa used are due to genetic factors, and progenies derived from crosses of these parental genotypes are useful for breeding programs related to plant architecture, physiological parameters and translocation of mineral nutrients...
2017: PloS One
https://www.readbyqxmd.com/read/28626552/a-new-microfluidic-approach-for-the-one-step-capture-amplification-and-label-free-quantification-of-bacteria-from-raw-samples
#4
Iago Pereiro, Amel Bendali, Sanae Tabnaoui, Lucile Alexandre, Jana Srbova, Zuzana Bilkova, Shane Deegan, Lokesh Joshi, Jean-Louis Viovy, Laurent Malaquin, Bruno Dupuy, Stéphanie Descroix
A microfluidic method to specifically capture and detect infectious bacteria based on immunorecognition and proliferative power is presented. It involves a microscale fluidized bed in which magnetic and drag forces are balanced to retain antibody-functionalized superparamagnetic beads in a chamber during sample perfusion. Captured cells are then cultivated in situ by infusing nutritionally-rich medium. The system was validated by the direct one-step detection of Salmonella Typhimurium in undiluted unskimmed milk, without pre-treatment...
February 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28626315/availability-of-the-two-step-test-to-evaluate-balance-in-frail-people-in-a-day-care-service
#5
Kazunori Kojima, Daisuke Kamai, Shogo Ishitani, Susumu Watanabe
[Purpose] This study evaluated balance tests in users of a day care service who needed nursing care or support, and investigated the usefulness of the Two-step Test for evaluating balance. [Subjects and Methods] The subjects were users of a day care service, and had certified need for long-term care or support. All subjects were able to undergo the balance evaluations. Balance tests included the 3-m Timed Up and Go test (TUG), the one-leg standing time, and the Two-step Test. [Results] The Two-step Test and other balance tests were strongly correlated...
June 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28626308/relationship-between-trunk-function-evaluated-using-the-trunk-righting-test-and-physical-function-in-patients-with-knee-osteoarthritis
#6
Kazuaki Kinoshita, Kazunari Ishida, Masashi Hashimoto, Yuki Yoneda, Yuta Naka, Hideyuki Kitanishi, Hiroki Oyagi, Yuichi Hoshino, Masahiro Kurosaka, Nao Shibanuma
[Purpose] The present study was to validate the importance of the trunk function evaluated by trunk righting test (TRT) with motor function in patients with knee osteoarthritis (OA) and to show the clinical use of TRT. [Subjects and Methods] This study included 50 patients with knee osteoarthritis who underwent total knee arthroplasty in our hospital. Correlations between physical functional test, such as muscle strength, balance function, and performance and TRT were statistically evaluated. [Results] The independent factors for ipsilateral TRT were maximal isometric knee extensor strength test and ipsilateral step test...
June 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28623941/development-of-a-program-theory-for-shared-decision-making-a-realist-review-protocol
#7
Gary Groot, Tamara Waldron, Tracey Carr, Linda McMullen, Lori-Ann Bandura, Shelley-May Neufeld, Vicky Duncan
BACKGROUND: The practicality of applying evidence to healthcare systems with the aim of implementing change is an ongoing challenge for practitioners, policy makers, and academics. Shared decision- making (SDM), a method of medical decision-making that allows a balanced relationship between patients, physicians, and other key players in the medical decision process, is purported to improve patient and system outcomes. Despite the oft-mentioned benefits, there are gaps in the current literature between theory and implementation that would benefit from a realist approach given the value of this methodology to analyze complex interventions...
June 17, 2017: Systematic Reviews
https://www.readbyqxmd.com/read/28623760/gait-dynamics-in-pisa-syndrome-and-camptocormia-the-role-of-stride-length-and-hip-kinematics
#8
C Tramonti, S Di Martino, E Unti, D Frosini, U Bonuccelli, B Rossi, R Ceravolo, C Chisari
This is an observational cross-sectional study evaluating gait dynamics in patients with Parkinson's Disease (PD) and severe postural deformities, PD without axial deviations and healthy subjects. Ten PS individuals with Pisa syndrome (PS) and nine subjects with Camptocormia (CC) performed 3-D Gait Analysis and were evaluated with walking and balance scales. Correlations with clinical and functional scales were investigated. Spatio-temporal and kinematic data were compared to ten PD subjects without postural deformities (PP) and ten healthy matched individuals (CG)...
June 10, 2017: Gait & Posture
https://www.readbyqxmd.com/read/28623390/disturbed-cervical-proprioception-affects-perception-of-spatial-orientation-while-in-motion
#9
Eva-Maj Malmström, Per-Anders Fransson, Terese Jaxmar Bruinen, Semir Facic, Fredrik Tjernström
The proprioceptive, visual and vestibular sensory systems interact to maintain dynamic stability during movement. The relative importance and interplay between these sensory systems is still not fully understood. Increased knowledge about spatial perception and postural orientation would provide better understanding of balance disorders, and their rehabilitation. Displacement of the body in space was recorded in 16 healthy subjects performing a sequence of stepping-in-place tests without any visual or auditory cues...
June 17, 2017: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
https://www.readbyqxmd.com/read/28623283/dosage-compensation-in-the-process-of-inactivation-reactivation-during-both-germ-cell-development-and-early-embryogenesis-in-mouse
#10
Xiaoyong Li, Zhiqiang Hu, Xuelin Yu, Chen Zhang, Binbin Ma, Lin He, Chaochun Wei, Ji Wu
Ohno proposed that dosage compensation in mammals evolved as a two-step mechanism involving X-inactivation and X-upregulation. While X-inactivation is well characterized, it remains to further analysis whether upregulation of the single activated X chromosome in mammals occurs. We obtained RNA-seq data, including single-cell RNA-seq data, from cells undergoing inactivation/reactivation in both germ cell development and early embryogenesis stages in mouse and calculated the X: A ratio from the gene expression...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622802/rapid-multiplex-dna-amplification-on-an-inexpensive-microdevice-for-human-identification-via-short-tandem-repeat-analysis
#11
Jacquelyn A DuVall, Delphine Le Roux, Brandon L Thompson, Christopher Birch, Daniel A Nelson, Jingyi Li, Daniel L Mills, An-Chi Tsuei, Martin G Ensenberger, Cindy Sprecher, Douglas R Storts, Brian E Root, James P Landers
Forensic DNA analysis requires several steps, including DNA extraction, PCR amplification, and separation of PCR fragments. Intuitively, there are numerous situations where it would be beneficial to speed up the overall DNA analysis process; in this work, we focus on the most time-consuming component in the analysis pipeline, namely the polymerase chain reaction (PCR). Primers were specially designed to target 10 human genomic loci, all yielding amplicons shorter than 350 bases, for ease of downstream integration with on-board microchip electrophoresis...
August 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28621919/insights-on-tuning-the-nanostructure-of-rgo-laminate-membranes-for-low-pressure-osmosis-process
#12
Qiuze Wang, Cyril Aubry, Yaxin Chen, Huaihe Song, Linda Zou
In this research, rGO laminates were prepared by a controlled partial reduction step, aimed to avoid aggregation and tune the interlayer spacing (d) between the rGO layers. The mild reducing agent vitamin C (L-AA) and cross-linker polycarboxilic acids were used to improve the stability of the assembled rGO laminate membranes. AFM was used for the first time to further investigate the statistical size distribution of spacing between rGO layers. Topographical images of the edges of the rGO layers were obtained with an AFM instrument, interlayer spacing profiles were extracted and then the data was plotted and fitted with Gaussian curves...
June 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28613231/melatonin-scavenger-properties-against-oxidative-and-nitrosative-stress-impact-on-gamete-handling-and-in-vitro-embryo-production-in-humans-and-other-mammals
#13
REVIEW
Pía Loren, Raúl Sánchez, María-Elena Arias, Ricardo Felmer, Jennie Risopatrón, Carolina Cheuquemán
Oxidative and nitrosative stress are common problems when handling gametes in vitro. In vitro development in mammalian embryos is highly affected by culture conditions, especially by reactive oxygen species (ROS) and reactive nitrogen species (RNS), because their absence or overproduction causes embryo arrest and changes in gene expression. Melatonin in gamete co-incubation during in vitro fertilization (IVF) has deleterious or positive effects, depending on the concentration used in the culture medium, demonstrating the delicate balance between antioxidant and pro-oxidant activity...
June 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28613066/balance-billing-by-health-care-providers-assessing-consumer-protections-across-states
#14
Kevin Lucia, Jack Hoadley, Ashley Williams
ISSUE: Privately insured consumers expect that if they pay premiums and use in-network providers, their insurer will cover the cost of medically necessary care beyond their cost-sharing. However, when obtaining care at emergency departments and in-network hospitals, patients treated by an out-of-network provider may receive an unexpected "balance bill" for an amount beyond what the insurer paid. With no explicit federal protections against balance billing, some states have stepped in to protect consumers from this costly and confusing practice...
June 2017: Issue Brief of the Commonwealth Fund
https://www.readbyqxmd.com/read/28608776/short-term-effectiveness-of-a-community-implemented-falls-prevention-referral-service
#15
Andrea Christoforou, Marietta L van der Linden, Pelagia Koufaki
PURPOSE: To evaluate the effectiveness of the 16-week evidence-based Steady Steps exercise referral scheme at improving physical function, balance confidence, and quality of life (QoL) of community-dwelling older adults at risk of falling. METHOD: A non-experimental, practice-based study involving a retrospective analysis of participant outcomes. Pre-post comparisons of three performance-based measures of gait and balance and of person-reported outcomes for balance confidence and QoL were performed...
June 13, 2017: Disability and Rehabilitation
https://www.readbyqxmd.com/read/28607951/pgc-1-coactivators-shepherding-the-mitochondrial-biogenesis-of-tumors
#16
Chi Luo, Hans R Widlund, Pere Puigserver
As coordinators of energy demands and nutritional supplies, the PGC-1 family of transcriptional coactivators regulates mitochondrial biogenesis to control the cellular bioenergetic state. Aside from maintaining normal and adapted cell physiology, recent studies indicate that PGC-1 coactivators also serve important functions in cancer cells. In fact, by balancing mitochondrial energy production with demands for cell proliferation, these factors are involved in almost every step of tumorigenesis. In this review, we discuss the interplay between PGC-1 coactivators and cancer pathogenesis, including tumor initiation, metastatic spread, and response to treatment...
October 2016: Trends in Cancer
https://www.readbyqxmd.com/read/28605727/parametric-study-for-the-optimization-of-ionic-liquid-pretreatment-of-corn-stover
#17
Gabriella Papa, Taya Feldman, Kenneth L Sale, Fabrizio Adani, Seema Singh, Blake A Simmons
A parametric study of the efficacy of the ionic liquid (IL) pretreatment (PT) of corn stover (CS) using 1-ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]) and cholinium lysinate ([Ch][Lys]) was conducted. The impact of 50% and 15% biomass loading for milled and non-milled CS on IL-PT was evaluated, as well the impact of 20 and 5mg enzyme/g glucan on saccharification efficiency. The glucose and xylose released were generated from 32 conditions - 2 ionic liquids (ILs), 2 temperatures, 2 particle sizes (S), 2 solid loadings, and 2 enzyme loadings...
May 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28604980/sealed-implantation-of-foldable-intraocular-lenses
#18
Dharmendra Nath, Eytan Z Blumenthal, Mohita Sharma, Michael Mimouni
PURPOSE: To evaluate the safety and efficacy of sealed implantation, a simple technique for implanting foldable intraocular lenses (IOLs). METHODS: This was a retrospective study including consecutive patients who underwent phacoemulsification cataract using the sealed implantation technique. Sealed implantation is a technique in which a single-piece, foldable IOL is implanted without an intraocular ophthalmic viscosurgical device (OVD) or continuous infusion (hydroimplantation) of balanced saline solution (BSS)...
June 10, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28603681/methane-emissions-from-the-marcellus-shale-in-southwestern-pennsylvania-and-northern-west-virginia-based-on-airborne-measurements
#19
Xinrong Ren, Dolly L Hall, Timothy Vinciguerra, Sarah E Benish, Phillip R Stratton, Doyeon Ahn, Jonathan R Hansford, Mark D Cohen, Sayantan Sahu, Hao He, Courtney Grimes, Ross J Salawitch, Sheryl H Ehrman, Russell R Dickerson
Natural gas production in the U.S. has increased rapidly over the past decade, along with concerns about methane (CH4) leakage (total fugitive emissions), and climate impacts. Quantification of CH4 emissions from oil and natural gas (O&NG) operations is important for establishing scientifically sound, cost-effective policies for mitigating greenhouse gases. We use aircraft measurements and a mass balance approach for three flight experiments in August and September 2015 to estimate CH4 emissions from O&NG operations in the southwestern Marcellus Shale region...
April 27, 2017: Journal of Geophysical Research. Atmospheres: JGR
https://www.readbyqxmd.com/read/28603464/reliability-and-validity-of-the-computerized-dynamic-posturography-sensory-organization-test-in-people-with-multiple-sclerosis
#20
Jeffrey R Hebert, Mark M Manago
BACKGROUND: People with multiple sclerosis (MS) frequently have impaired postural control (balance). Psychometric properties of clinical tests of balance for individuals with MS, including the computerized dynamic posturography sensory organization test (CDP-SOT), are poorly understood. This study aimed to determine the reliability and discriminant validity of the CDP-SOT in people with MS. METHODS: The CDP-SOT was performed on 30 participants with MS. A 2-week-interval, repeated-measures (sessions 1 and 2) design was implemented to investigate test-retest reliability of the CDP-SOT and the ability of the CDP-SOT to discriminate between participants with lower versus higher disability...
May 2017: International Journal of MS Care
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