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Lucie Pinasseau, Arnaud Verbaere, Maryline Roques, Emmanuelle Meudec, Anna Vallverdú-Queralt, Nancy Terrier, Jean-Claude Boulet, Véronique Cheynier, Nicolas Sommerer
A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour...
October 21, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Abhijeet B Shelke, Rajeev Menon, Anuj Kapadiya, Sachin Yalagudri, Daljeet Saggu, Sandeep Nair, C Narasimhan
OBJECTIVE: Alcohol septal ablation (ASA) is a therapeutic alternative to surgical myectomy in patients with hypertrophic obstructive cardiomyopathy (HOCM). However, the anatomical variability of the septal branch, risk of complete heart block, and late onset ventricular arrhythmias are limitations to its therapeutic usage. There is recent interest in the use of radiofrequency catheter ablation (RFCA) as a therapeutic option in HOCM. We aimed to assess the safety and efficacy of RFCA in the treatment of symptomatic HOCM...
September 2016: Indian Heart Journal
Remedios Guzmán-Guillén, Alexandre Campos, Joana Machado, Marisa Freitas, Joana Azevedo, Edgar Pinto, Agostinho Almeida, Ana M Cameán, Vitor Vasconcelos
Natural toxins produced by freshwater cyanobacteria, such as cylindrospermopsin, have been regarded as an emergent environmental threat. Despite the risks for food safety, the impact of these water contaminants in agriculture is not yet fully understood. Carrots (Daucus carota) are root vegetables, extensively consumed worldwide with great importance for human nourishment and economy. It is, therefore, important to evaluate the possible effects of using water contaminated with cyanotoxins on carrot cultivation...
October 21, 2016: Ecotoxicology
Zhejun Wang, Hazuki Maezono, Ya Shen, Markus Haapasalo
INTRODUCTION: The purpose of this study was to examine the level of erosion in root dentin caused by different irrigation methods and protocols. METHODS: Thirty-five extracted upper molar teeth were instrumented and divided into 7 groups to undergo treatment by different methods: negative control, GentleWave System (Sonendo Inc, Laguna Hills, CA), and syringe needle irrigation following different protocols. The teeth were instrumented to size #25/.08 or #30/.09 for needle irrigation groups and to ProTaper size S1 for the GentleWave group under 5% sodium hypochlorite (NaOCl)...
October 18, 2016: Journal of Endodontics
Tommaso Michele Moles, Antonio Pompeiano, Thais Huarancca Reyes, Andrea Scartazza, Lorenzo Guglielminetti
Landraces represent an important part of the biodiversity well-adapted under limiting environmental conditions. We investigated the response of two Southern Italy tomato landraces, the well-known San Marzano (our commercial standard) and a local accession called "Ciettaicale", to different levels of sodium chloride in water irrigation (from 0 up to 600 mM) for a short-time exposure (one week). The combination of the chlorophyll a fluorescence and gas exchange analyses suggested that Ciettaicale maintained a higher efficiency of photosystem II (PSII) photochemistry and CO2 utilization at high salinity concentrations than San Marzano...
October 12, 2016: Plant Physiology and Biochemistry: PPB
Abidelfatah M Nasser, Neta-Lee Benisti, Naomi Ofer, Sivan Hovers, Yeshayahu Nitzan
Advanced wastewater treatment processes are applied to prevent the environmental dissemination of pathogenic microorganisms. Giardia lamblia causes a severe disease called giardiasis, and is highly prevalent in untreated wastewater worldwide. Monitoring the microbial quality of wastewater effluents is usually based on testing for the levels of indicator microorganisms in the effluents. This study was conducted to compare the suitability of fecal coliforms, F+ coliphages and sulfide reducing clostridia (SRC) as indicators for the reduction of Giardia cysts in two full-scale wastewater treatment plants...
October 21, 2016: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Wei Phin Tan, Ulku C Turba, Leslie A Deane
INTRODUCTION: We describe a case of renal pelvi-ureteric fungus ball managed with placement of two nephrostomy tubes and amphotericin B irrigation through a nephrostomy tube with the other to free drain. CASE REPORT: A 46-year-old man with uncontrolled Type 2 diabetes mellitus was referred to the urology clinic for workup of recurrent urinary tract infection. Urine culture grew Candida albicans. The patient was started on oral fluconazole therapy. Cystoscopy and cystogram revealed a grade 3 left vesicoureteral reflux and right retrograde pyelogram revealed a filling defect in the right renal pelvis extending into the proximal ureter with severe hydroureteronephrosis...
October 15, 2016: Urologia
Roy George
Data sourcesCochrane Central Register of Controlled Trials, Medline, Embase and Scopus databases.Study selectionStudies that reported the precision of electronic apex locators (EALs) in locating the apical constriction (AC) in primary root canal treatment of human teeth compared with a histologic evaluation of the AC were considered.Data extraction and synthesisData were extracted and quality assessed independently by two reviewers.ResultsTen studies were included, reporting on 1105 EAL measurements. Seven studies were considered to be at high risk of bias and three at low risk...
September 2016: Evidence-based Dentistry
Giuseppe Lo Giudice, Angelo Lizio, Roberto Lo Giudice, Antonio Centofanti, Giuseppina Rizzo, Michele Runci, Angela Alibrandi, Marco Cicciù
Aim. Purpose of the present paper is to analyze the efficiency of different post-space irrigation protocols. Methods. 28 single rooted teeth were endodontically treated. After post-space preparation every sample was assigned to one of three experimental groups and to one control group. In each group different irrigation protocols were performed as follows: EDTA (Group A), 37% orthophosphoric acid (Group B), and EDTA + 37% orthophosphoric acid with ultrasounds activation (Group C). In the control group (Group D) the irrigate association was not activated by ultrasounds...
2016: International Journal of Dentistry
Guigang Li, Jingmin Guo, Rong Liu, Weikun Hu, Lingjuan Xu, Juan Wang, Subo Cai, Hong Zhang, Yingting Zhu
Background: To explore the prevalence of lacrimal duct obstruction in patients with infectious keratitis, and the necessity of lacrimal duct dredge in the treatment of human infectious keratitis. Methodology/Principle Findings: The design is prospective, non-control case series. Thirty-one eyes from twenty-eight continuous patients with infectious keratitis were included in this study. The presence/absence of lacrimal duct obstruction was determined by the lacrimal duct irrigation test. The diagnosis of infectious keratitis was made based on clinical manifestations, cornea scraping microscopic examination and bacterial/fungus culture...
2016: International Journal of Medical Sciences
Jinlu Yu, Yunbao Guo, Baofeng Xu, Kan Xu
The middle meningeal artery (MMA) is a very important artery in neurosurgery. Many diseases, including dural arteriovenous fistula (DAVF), pseudoaneurysm, true aneurysm, traumatic arteriovenous fistula (AVF), moyamoya disease (MMD), recurrent chronic subdural hematoma (CSDH), migraine and meningioma, can involve the MMA. In these diseases, the lesions occur in either the MMA itself and treatment is necessary, or the MMA is used as the pathway to treat the lesions; therefore, the MMA is very important to the development and treatment of a variety of neurosurgical diseases...
2016: International Journal of Medical Sciences
Yung-Hao Liu, Pai-Shan Chen, Shang-Da Huang
Shaker-assisted dispersive liquid-liquid microextraction (SA-DLLME) and surfactant dispersive liquid-liquid microextraction (SDLLME) have been developed to determine five diphenylether herbicides in water samples using high-performance liquid chromatography with photodiode array detection (HPLC-PDA). For SA-DLLME, an up-and-down shaker-assisted emulsification was used. Extraction was complete in 3 min. Only 30 μL of decyl acetate was required, without a dispersive solvent. The linear range was from 2 to 1000 μg L(-1), the coefficient of determination (r (2)) was better than 0...
October 19, 2016: Journal of AOAC International
Belén Martínez-Alcántara, Mary-Rus Martínez-Cuenca, Almudena Bermejo, Francisco Legaz, Ana Quiñones
The main objective of this study was to compare the performance of two liquid organic fertilizers, an animal and a plant-based fertilizer, with mineral fertilization on citrus trees. The source of the fertilizer (mineral or organic) had significant effect in the nutritional status of the organic and conventionally managed mandarins. Nutrient uptake, vegetative growth, carbohydrate synthesis and soil characteristics were analyzed. Results showed that plants fertilized with animal based liquid fertilizers exhibited higher total biomass with a more profuse development of new developing organs (leaves and fibrous roots)...
2016: PloS One
Alberto Dagna, Giulia Gastaldo, Riccardo Beltrami, Claudio Poggio
This study evaluated the root canal dentine surface by scanning electron microscope (SEM) after shaping with two reciprocating single-file NiTi systems and two rotating single-file NiTi systems, in order to verify the presence/absence of the smear layer and the presence/absence of open tubules along the walls of each sample; Forty-eight single-rooted teeth were divided into four groups and shaped with OneShape (OS), F6 SkyTaper (F6), WaveOne (WO) and Reciproc and irrigated using 5.25% NaOCl and 17% EDTA. Root canal walls were analyzed by SEM at a standard magnification of 2500×...
October 17, 2016: Journal of Functional Biomaterials
Zafar Iqbal Khan, Kafeel Ahmad, Sidrah Rehman, Samra Siddique, Humayun Bashir, Asma Zafar, Muhammad Sohail, Salem Alhajj Ali, Eugenio Cazzato, Giuseppe De Mastro
In the recent years, the use of sewage water for irrigation has attracted the attention of arid and semi-arid countries where the availability of fresh water is poor. Despite the potential use of sewage water in crop irrigation as effective and sustainable strategy, the environmental and human risks behind this use need to be deeply investigated. In this regard, an experiment was carried out under field conditions in Nursery, University College of Agriculture Sargodha, to evaluate the possible health risks of undesirable metals in wheat grains...
October 20, 2016: Environmental Science and Pollution Research International
Artur Baszko, Wojciech Telec, Piotr Kałmucki, Piotr Iwachów, Karol Kochman, Radosław Szymański, Jan Kłopocki, Stefan Ożegowski, Andrzej Szyszka, Tomasz Siminiak
Bipolar radiofrequency (RF) ablation is effective in treatment of ventricular tachycardia originating from thick interventricular septum. The RF generator and CARTO system can be used to precisely and safely perform ablation. Standard ablation catheter can be used with indifferent ablation electrode connected to the electrode receptacle in RF generator with custom-made cable.
October 2016: Clinical Case Reports
Volkan Etus, Gokmen Kahilogullari, Hakan Karabagli, Agahan Unlu
AIM: Neonatal intraventricular hemorrhage (IVH) usually results in posthemorrhagic hydrocephalus (PHH). This multicenter study describes the approach of early neuroendoscopic ventricular irrigation for the treatment of IVH/PHH and compares the results with the cases that have been initially treated only with conventional temporary CSF diversion techniques. MATERIAL AND METHODS: The data of total 74 neonatal PHH cases that have been treated at three pediatric neurosurgery centers were retrospectively analyzed...
August 30, 2016: Turkish Neurosurgery
Ru-Sen Zhu, Yi-Ming Ren, Jian-Jun Yuan, Zi-Jian Cui, Jun Wan, Bao-You Fan, Wei Lin, Xian-Hu Zhou, Xue-Li Zhang
Lumbar disc herniation (LDH) is a common disease and lumbar discectomy is the most common surgical procedure carried out for patients with low back pain and leg symptoms. Although most researchers are focusing on the surgical techniques during operation, the aim of this study is to evaluate the effect of local intervertebral lavage during microdiscectomy.In this retrospective study, 410 patients were operated on by microdiscectomy for LDH during 2011 to 2014. Retrospectively, 213 of them (group A) accepted local intervertebral irrigation with saline water before wound closure and 197 patients (group B) only had their operative field irrigated with saline water...
October 2016: Medicine (Baltimore)
Vahit Guzelburc, Mehmet Balasar, Mukaddes Colakogullari, Selcuk Guven, Abdulkadir Kandemir, Ahmet Ozturk, Pelin Karaaslan, Bulent Erkurt, Selami Albayrak
PURPOSE: Irrigation-induced increase in intrarenal pressure is of concern because it may cause infection due to increased pyelovenous and pyelolymphatic absorption. This study is the first to compare prospectively the absorbed fluid volumes during percutaneous nephrolithotomy (PCNL) and retrograde intrarenal surgery (RIRS) for stones larger than 2 cm. MATERIALS AND METHODS: General anesthesia was applied to all patients. Isotonic solution containing 1 % ethanol was used as irrigation fluid...
2016: SpringerPlus
Or Friedman, Ehud Arad, Oded Ben Amotz
In the pursuit of success in sports, some athletes are not deterred by health risks associated with the (mis)use of black market preparations of dubious origin as performance-enhancing agents. Several studies published in the recent years demonstrated that anabolic-androgenic steroids, but also stimulants and growth hormones, are misused by numerous recreational athletes from all over the world. Trenbolone is an anabolic steroid routinely used in the finishing phase of beef production to improve animal performance and feed efficiency...
September 2016: Plastic and Reconstructive Surgery. Global Open
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