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https://www.readbyqxmd.com/read/28919685/prevalence-of-listeria-monocytogenes-yersinia-enterocolitica-staphylococcusaureus-and-salmonella-enterica-typhimurium-in-meat-and-meat-products-using-multiplex-polymerase-chain-reaction
#1
C Latha, C J Anu, V J Ajaykumar, B Sunil
AIM: The objective of the study was to investigate the occurrence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcusaureus, and Salmonella enterica Typhimurium in meat and meat products using the multiplex polymerase chain reaction (PCR) method. MATERIALS AND METHODS: The assay combined an enrichment step in tryptic soy broth with yeast extract formulated for the simultaneous growth of target pathogens, DNA isolation and multiplex PCR. A total of 1134 samples including beef (n=349), chicken (n=325), pork (n=310), chevon (n=50), and meat products (n=100) were collected from different parts of Kerala, India...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28919035/development-of-a-fast-and-efficient-method-for-hepatitis-a-virus-concentration-from-green-onion
#2
Yan Zheng, Yuan Hu
Hepatitis A virus (HAV) can cause serious liver disease and even death. HAV outbreaks are associated with the consumption of raw or minimally processed produce, making it a major public health concern. Infections have occurred despite the fact that effective HAV vaccine has been available. Development of a rapid and sensitive HAV detection method is necessary for an investigation of an HAV outbreak. Detection of HAV is complicated by the lack of a reliable culture method. In addition, due to the low infectious dose of HAV, these methods must be very sensitive...
September 14, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28887421/impact-of-raised-without-antibiotics-beef-cattle-production-practices-on-occurrences-of-antimicrobial-resistance
#3
Amit Vikram, Pablo Rovira, Getahun E Agga, Terrance M Arthur, Joseph M Bosilevac, Tommy L Wheeler, Paul S Morley, Keith E Belk, John W Schmidt
The specific antimicrobial resistance (AMR) decreases that can be expected from reducing antimicrobial (AM) use in United States (US) beef production have not been defined. To address this data gap, feces were recovered from 36 lots of "raised without antibiotics" (RWA) and 36 lots of "conventional" (CONV) beef cattle. Samples (N = 719) were collected during harvest and distributed over a year. AMR was assessed by: i) culture of six AM-resistant bacteria (ARB); ii) qPCR for 10 AMR genes (ARGs); iii) an qPCR array of 84 ARGs; and iv) metagenomic sequencing...
September 8, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28873602/application-of-chemometrics-to-assess-the-influence-of-ultrasound-frequency-lactobacillus-sakei-culture-and-drying-on-beef-jerky-manufacture-impact-on-amino-acid-profile-organic-acids-texture-and-colour
#4
K Shikha Ojha, Daniel Granato, Gaurav Rajuria, Francisco J Barba, Joseph P Kerry, Brijesh K Tiwari
The effects of ultrasound (US) frequency, addition of Lactobacillus sakei culture and drying time on key nutritional (protein, amino acids, and organic acids) and physicochemical properties (texture and colour) of cultured and uncultured beef jerky were evaluated. Cultured and uncultured jerky samples were subjected to US frequencies of 25kHz, 33kHz and 45kHz for 30min prior to marination and drying. Principal component analysis demonstrated a significant effect of beef jerky processing conditions on physicochemical properties...
January 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28842275/preferential-isolation-of-megasphaera-elsdenii-from-pig-feces
#5
Yu Kajihara, Shota Yoshikawa, Yuichiro Cho, Toshiyuki Ito, Hirokuni Miyamoto, Hiroaki Kodama
Lactic acid produced by intestinal bacteria is fermented by lactate-utilizing bacteria. In this study, we developed a selective culture medium (KMI medium) for Megasphaera elsdenii, a lactate-utilizing bacterium that is abundant in pig intestines. Supplementation of the medium with lactate and beef extract powder was necessary for the preferential growth of M. elsdenii. In addition, we designed a species-specific primer set to detect M. elsdenii. When pig fecal samples were plated on KMI agar medium, approximately 60-100% of the resulting colonies tested positive using the M...
August 28, 2017: Anaerobe
https://www.readbyqxmd.com/read/28807288/prevalence-of-tritrichomonas-foetus-in-tennessee-bulls
#6
Chika C Okafor, Lew G Strickland, Brittni M Jones, Stephen Kania, David E Anderson, Brian K Whitlock
The prevalence of bovine trichomonosis (BT) in TN bulls was estimated through both active screening of bulls and review of previous laboratory records. During the active bull screening, preputial smegma specimens were collected from 458 TN beef bulls at 2 cattle slaughterhouses and 2 stockyards, which serve most beef bulls in TN, between March 2014 and June 2015. Each specimen was cultured for Tritrichomonas foetus (T. foetus) as well as evaluated microscopically every other day for seven days for any protozoa resembling T...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28803866/pericarditis-caused-by-campylobacter-fetus-subspecies-fetus-associated-with-ingestion-of-raw-beef-liver
#7
Yoshihiko Kiyasu, Daiki Akiyama, Yoko Kurihara, Hiroshi Koganemaru, Shigemi Hitomi
Campylobacter fetus is an organism residing primarily in the gastrointestinal tracts of cattle and sheep and transmitting to humans through ingestion of contaminated food products or surface water. The organism has caused various extraintestinal infections but, to date, purulent pericarditis due to the organism has rarely been described. We report a case of purulent pericarditis due to C. fetus subsp. fetus, occurring in a patient having several predisposing conditions, including receiving hemodialysis therapy, recent surgery for cecal cancer, and administration of esomeprazole...
August 10, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28773945/application-of-a-loop-type-laboratory-biofilm-reactor-to-the-evaluation-of-biofilm-for-some-metallic-materials-and-polymers-such-as-urinary-stents-and-catheters
#8
Hideyuki Kanematsu, Hikonaru Kudara, Shun Kanesaki, Takeshi Kogo, Hajime Ikegai, Akiko Ogawa, Nobumitsu Hirai
A laboratory biofilm reactor (LBR) was modified to a new loop-type closed system in order to evaluate novel stents and catheter materials using 3D optical microscopy and Raman spectroscopy. Two metallic specimens, pure nickel and cupronickel (80% Cu-20% Ni), along with two polymers, silicone and polyurethane, were chosen as examples to ratify the system. Each set of specimens was assigned to the LBR using either tap water or an NB (Nutrient broth based on peptone from animal foods and beef extract mainly)-cultured solution with E-coli formed over 48-72 h...
October 11, 2016: Materials
https://www.readbyqxmd.com/read/28739600/whole-genome-sequencing-and-concordance-between-antimicrobial-susceptibility-genotypes-and-phenotypes-of-bacterial-isolates-associated-with-bovine-respiratory-disease
#9
Joseph R Owen, Noelle Noyes, Amy E Young, Daniel J Prince, Patricia C Blanchard, Terry W Lehenbauer, Sharif S Aly, Jessica H Davis, Sean M O'Rourke, Zaid Abdo, Keith Belk, Michael R Miller, Paul Morley, Alison L Van Eenennaam
Extended laboratory culture and antimicrobial susceptibility testing timelines hinder rapid species identification and susceptibility profiling of bacterial pathogens associated with bovine respiratory disease, the most prevalent cause of cattle mortality in the United States. Whole-genome sequencing offers a culture-independent alternative to current bacterial identification methods, but requires a library of bacterial reference genomes for comparison. To contribute new bacterial genome assemblies and evaluate genetic diversity and variation in antimicrobial resistance genotypes, whole-genome sequencing was performed on bovine respiratory disease-associated bacterial isolates (Histophilus somni, Mycoplasma bovis, Mannheimia haemolytica, and Pasteurella multocida) from dairy and beef cattle...
September 7, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28717239/a-novel-pathogen-capturing-device-for-removal-and-detection
#10
Gwangseong Kim, Horatiu Vinerean, Angelo Gaitas
A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. By recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher concentration of pathogens in a small volume. The described method can provide an effective and economical solution to microbiology techniques that rely on enrichment, thereby expediting diagnostics. Using this method 80...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28696055/practices-and-exploration-on-competition-of-molecular-biological-detection-technology-among-students-in-food-quality-and-safety-major
#11
Yaning Chang, Yuke Peng, Pengfei Li, Yingping Zhuang
With the increasing importance in the application of the molecular biological detection technology in the field of food safety, strengthening education in molecular biology experimental techniques is more necessary for the culture of the students in food quality and safety major. However, molecular biology experiments are not always in curricula of Food quality and safety Majors. This paper introduced a project "competition of molecular biological detection technology for food safety among undergraduate sophomore students in food quality and safety major", students participating in this project needed to learn the fundamental molecular biology experimental techniques such as the principles of molecular biology experiments and genome extraction, PCR and agarose gel electrophoresis analysis, and then design the experiments in groups to identify the meat species in pork and beef products using molecular biological methods...
July 8, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28693300/investigation-of-bovine-tuberculosis-outbreaks-using-the-trace-back-system-and-molecular-typing-in-korean-hanwoo-beef-cattle
#12
Bok Kyung Ku, Bo-Young Jeon, Jae Myung Kim, Young-Boo Jang, Hyeyoung Lee, Jae Young Choi, Suk Chan Jung, Hyang-Mi Nam, Hun Park, Sang-Nae Cho
Bovine tuberculosis is a chronic contagious disease responsible for major agricultural economic losses. Abattoir monitoring and trace-back systems are an appropriate method to control bovine tuberculosis, particularly in beef cattle. In the present study, a trace-back system was applied to bovine tuberculosis outbreaks of Korean native Hanwoo beef cattle. Bovine tuberculosis was detected in three index beef cattle during abattoir monitoring in Jeonbuk Province, Korea, and the original herds were traced back from each index cattle...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28686492/effect-of-equilibrated-ph-and-indigenous-spoilage-microorganisms-on-the-inhibition-of-proteolytic-clostridium-botulinum-toxin-production-in-experimental-meals-under-temperature-abuse
#13
Max C Golden, Brandon J Wanless, Jairus R D David, D Scott Lineback, Ryan J Talley, Bala Kottapalli, Kathleen A Glass
Clostridium botulinum is a foreseeable biological hazard in prepared refrigerated meals that needs to be addressed in food safety plans. The objective of this study was to evaluate the effect of product composition and storage temperature on the inhibition of botulinum toxin formation in nine experimental meals (meat, vegetable, or carbohydrate based). Treatments were inoculated with proteolytic C. botulinum, vacuum packaged, cooked at 90°C for 10 min, and assayed for botulinum toxin in samples stored at 25°C for up to 96 h for phase 1, or at 25°C for 12 h and then transferred to 12...
July 7, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28669383/field-performance-of-six-mycobacterium-avium-subsp-paratuberculosis-antigens-in-a-20h-interferon-gamma-release-assay-in-belgium
#14
Kathy Dernivoix, Virginie Roupie, Sarah Welby, Sophie Roelandt, Sophie Viart, Jean-Jacques Letesson, Ruddy Wattiez, Kris Huygen, Marc Govaerts
Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic granulomatous enteritis which primarily affects domestic and wild ruminants, resulting in serious economic losses for dairy and beef industry around the world. There is no satisfactory cure or vaccine, and actual diagnostic tests need improvement, particularly for the initial stages of the disease. Map specific cell-mediated immune responses may allow early detection of the infection at subclinical stages. In this study, over a period of 39 months, we collected 548 blood samples in two culture-confirmed Map-infected herds, 95 blood samples in five dairy herds that scored negative during 3 consecutive years of Map serology testing and 79 samples in three culture-confirmed M...
July 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28656639/comparison-of-immunomagnetic-separation-beads-for-detection-of-six-non-o157-shiga-toxin-producing-escherichia-coli-serogroups-in-different-matrices
#15
A L Kraft, D W Lacher, W L Shelver, J S Sherwood, T M Bergholz
Immunomagnetic separation used with culture based methods has been a useful technique in the detection of pathogens. However, previous studies have not answered many of the necessary questions for real world applications. The objective of this study was to assess the efficacy of different immunomagnetic separation (IMS) bead types in recovery of the correct serogroup from a mixture of big six non-O157 Shiga toxin-producing Escherichia coli strains. To determine the impact of different matrices on recovery, samples of sterile phosphate buffered saline (PBS), sterile and non-sterile cattle faeces, ground beef and lettuce were inoculated with 10 CFU per ml mixture of isolates representing the six serogroups...
June 28, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28649235/detection-of-mycobacteria-by-culture-and-dna-based-methods-in-animal-derived-food-products-purchased-at-spanish-supermarkets
#16
Iker A Sevilla, Elena Molina, Maitane Tello, Natalia Elguezabal, Ramón A Juste, Joseba M Garrido
Mycobacteria include obligate and opportunistic pathogens that cause significant human and animal disease. The burden of tuberculosis has been largely reduced in developed territories but remains a huge problem worldwide. The significance of nontuberculous mycobacteria is growing considerably, especially in developed regions with higher life expectancy and more therapy-related immunosuppressed individuals. Due to their robustness mycobacteria can contaminate animal products by direct transmission from infected individuals or by environmental contamination during processing...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28631590/comparative-evaluation-of-veriflow-%C3%A2-salmonella-species-to-usda-and-fda-culture-based-methods-for-the-detection-of-salmonella-spp-in-food-and-environmental-samples
#17
Amrita Puri, Adam C Joelsson, Shawn P Terkhorn, Ashley S Brown, Zara E Gaudioso, Nicholas A Siciliano
Veriflow® Salmonella species (Veriflow SS) is a molecular-based assay for the presumptive detection of Salmonella spp. from environmental surfaces (stainless steel, sealed concrete, plastic, and ceramic tile), dairy (2% milk), raw meat (20% fat ground beef), chicken carcasses, and ready-to-eat (RTE) food (hot dogs). The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post-PCR amplification and requires only an 18 h enrichment for maximum sensitivity...
September 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28552545/prevalence-of-tuberculous-lymphadenitis-in-slaughtered-cattle-in-eastern-cape-south-africa
#18
Nolwazi L Bhembe, Ishmael F Jaja, Uchechukwu U Nwodo, Anthony I Okoh, Ezekiel Green
OBJECTIVE: To detect the prevalence of Mycobacterium tuberculosis complex (MTBC) in the lymph nodes of slaughtered cattle collected from selected abattoirs in Eastern Cape Province of South Africa. METHODS: A total of 376 lymph nodes were collected from slaughtered cattle over a period of 12 months. Certain characteristics (sex, age, body condition score, and breed) were observed to be associated with MTBC among slaughtered cattle. Collected samples were cultured and tested for acid-fast bacilli (AFB)...
May 24, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28551188/regulatory-effect-of-dietary-intake-of-chromium-propionate-on-the-response-of-monocyte-derived-macrophages-from-holstein-cows-in-mid-lactation
#19
M Garcia, Y Qu, C M Scholte, D O'Connor, W Rounds, K M Moyes
Chromium (Cr) has been reported to enhance immune function and improve insulin sensitivity and performance in beef and dairy cattle. However, its effect on bovine macrophage inflammatory and metabolic response is unknown. The objective of this study was to characterize the effect of dietary Cr on the inflammatory and metabolic response of polarized macrophages ex vivo. Twelve primiparous and 16 multiparous healthy Holstein cows in mid lactation (143 ± 37 d in milk) were enrolled in this study. Cows were fed a common total mixed ration once per day that was top-dressed with 200 g of ground corn containing 1 of 2 dietary treatments: control (CTL, no Cr supplementation) or Cr propionate (CrP, 8 mg of Cr/cow per day) for 35 d...
May 24, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28550057/prevalence-and-genomic-characterization-of-escherichia-coli-o157-h7-in-cow-calf-herds-throughout-california
#20
Jay N Worley, Kristopher A Flores, Xun Yang, Jennifer A Chase, Guojie Cao, Shuai Tang, Jianghong Meng, Edward R Atwill
Escherichia coli serotype O157:H7 is a zoonotic food- and waterborne bacterial pathogen that causes a high hospitalization rate and can cause life-threatening complications. Increasingly, E. coli O157:H7 infections appear to originate from fresh produce. Ruminants, such as cattle, are a prominent reservoir of E. coli O157:H7 in the United States. California is one of the most agriculturally productive regions in the world for fresh produce, beef, and milk. The close proximity of fresh produce and cattle presents food safety challenges on a uniquely large scale...
August 15, 2017: Applied and Environmental Microbiology
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