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https://www.readbyqxmd.com/read/29279817/multi-level-statistical-optimization-of-l-asparaginase-from-bacillus-subtilis-vuvd001
#1
Rajeswara Reddy Erva, T C Venkateswarulu, Bangaraiah Pagala
Physical and chemical factors influencing the anti-leukemic l-asparaginase enzyme production by Bacillus subtilis VUVD001 were optimized using multi-stage optimization on the basis of preliminary experimental outcomes obtained by conventional one-factor-at-a-time approach using shake flasks. Process variables namely carbon, nitrogen sources, pH and temperature were taken into consideration during response surface methodology (RSM) optimization. The finest enzyme activity of 0.51 IUml-1 obtained by OFAT method was enhanced by 3...
January 2018: 3 Biotech
https://www.readbyqxmd.com/read/29279092/weight-gain-and-resistance-to-gastrointestinal-nematode-infections-in-two-genetically-diverse-groups-of-cattle
#2
Johan Höglund, Anna Hessle, Konstantinos Zaralis, Katarina Arvidsson-Segerkvist, Spiridoula Athanasiadou
Body weight gain (BWG) and gastrointestinal nematode challenge (GIN) were investigated in two genetically diverse groups of cattle. Thirty-two dairy calves (D=Swedish Red/Holstein) and 31 dairy×beef crosses (C=Swedish Red/Holstein×Charolais) pairwise matched by dam breed and birth dates, were monitored for ≈20 weeks on a pasture grazed by cattle in the previous year. At turn-out, animals (between 6 and 12 months age) from each genotype were either infected with 5000 third stage (L3) Ostertagia ostertagi (50%) and Cooperia oncophora (50%) larvae (H, high-exposure); or treated monthly with 0...
January 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29276663/detection-of-viable-but-non-culturable-escherichia-coli-o157-h7-by-pcr-in-combination-with-propidium-monoazide
#3
Junliang Zhong, Xihong Zhao
The aim of this study was to evaluate the applicability of the conventional PCR detection method combined with propidium monoazide (PMA) treatment for the detection of viable but non-culturable (VBNC) state Escherichia coli O157:H7 in ground beef meatballs. Under low temperature, E. coli O157:H7 cells were induced into the VBNC state in ground beef meatballs at - 20 °C after 152 days. The optimal PMA concentration of 5 µg/mL was obtained in beef meatball samples, which could completely inhibit the DNA amplification on dead cells (106 cells/mL) but with no inhibition on viable cells...
January 2018: 3 Biotech
https://www.readbyqxmd.com/read/29275223/antimicrobial-residues-and-resistance-against-critically-important-antimicrobials-in-non-typhoidal-salmonella-from-meat-sold-at-wet-markets-and-supermarkets-in-vietnam
#4
Nguyen Thi Nhung, Nguyen Thi Bich Van, Nguyen Van Cuong, Truong Thi Quy Duong, Tran Thi Nhat, Tran Thi Thu Hang, Nguyen Thi Hong Nhi, Bach Tuan Kiet, Vo Be Hien, Pham Thi Ngoc, James Campbell, Guy Thwaites, Juan Carrique-Mas
Excessive antimicrobial usage and deficiencies in hygiene in meat production systems may result in undesirable human health hazards, such as the presence of antimicrobial drug residues and non-typhoidal Salmonella (NTS), including antimicrobial resistant (AMR) NTS. Recently, Vietnam has witnessed the emergence of integrated intensive animal production systems, coexisting with more traditional, locally-sourced wet markets. To date no systematic studies have been carried out to compare health hazards in beef, pork and chicken in different production systems...
December 17, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29274052/epidemiology-and-effect-of-gastrointestinal-nematodes-on-beef-cattle-from-tropical-argentina
#5
V H Suarez, G M Martínez, J F Micheloud, A E Viñabal
The aim of this work was to study the epidemiology and the harmful effect of gastrointestinal nematodes (GINs) on beef cattle in the piedmont forest and grassland region of northwestern Argentina, between March 2015 and March 2016. Sixty weaned female calves were divided into three groups: STG-calves treated systematically with 200 mcg/kg moxidectin every 40-50 days; LTG-calves treated as usually locally implemented, with 200 mcg/kg ivermectin on May 4 and August 5; and UTG-calves not receiving treatment...
December 22, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29246181/detection-and-identification-of-antimicrobial-resistant-salmonella-in-raw-beef-at-wolaita-sodo-municipal-abattoir-southern-ethiopia
#6
Wondimu Wabeto, Yishak Abraham, Antehun Alemayehu Anjulo
BACKGROUND: The consumption of multidrug resistant Salmonella isolates along with a raw meat dish is directly relevant to the global public health crisis of antimicrobial resistance. All countries around the globe are suffering from food-borne diseases. In developing countries, more than one billion individuals suffering from gastroenteritis and around five million infected individuals die annually. METHODS: A cross-sectional study was carried out from December 2015 to May 2016 to show the risk of Salmonella associated with consuming traditional raw meat dishes and to characterize the antimicrobial resistance profile at Wolaita Sodo municipal abattoir...
December 16, 2017: Journal of Health, Population, and Nutrition
https://www.readbyqxmd.com/read/29244862/application-of-system-dynamics-and-participatory-spatial-group-model-building-in-animal-health-a-case-study-of-east-coast-fever-interventions-in-lundazi-and-monze-districts-of-zambia
#7
Chisoni Mumba, Eystein Skjerve, Magda Rich, Karl M Rich
East Coast Fever (ECF) is the most economically important production disease among traditional beef cattle farmers in Zambia. Despite the disease control efforts by the government, donors, and farmers, ECF cases are increasing. Why does ECF oscillate over time? Can alternative approaches such as systems thinking contribute solutions to the complex ECF problem, avoid unintended consequences, and achieve sustainable results? To answer these research questions and inform the design and implementation of ECF interventions, we qualitatively investigated the influence of dynamic socio-economic, cultural, and ecological factors...
2017: PloS One
https://www.readbyqxmd.com/read/29225756/prevalence-of-clostridium-difficile-and-its-toxigenic-genotype-in-beef-samples-in-west-of-iran
#8
Malihe Kheradmand, Somayeh Jalilian, Amirhooshang Alvandi, Ramin Abiri
Background and Objectives: Clostridium difficile is the leading cause of nosocomial diarrhea and pseudomembranous colitis. The prevalence of C. difficile infection differs in various geographical areas. The aim of this study was to determine the prevalence of C. difficile isolates and the prevalence of cdd3, tcdA and tcdB genes in beef samples in Kermanshah Province. Materials and Methods: One hundred ground beef samples were randomly collected from the butchers of Kermanshah province during March 2014 to March 2015...
June 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29196005/inactivation-of-escherichia-coli-o157-h7-and-bacillus-cereus-by-power-ultrasound-during-the-curing-processing-in-brining-liquid-and-beef
#9
Dacheng Kang, Yuhe Jiang, Lujuan Xing, Guanghong Zhou, Wangang Zhang
The objective of this study was to evaluate the effects of ultrasound on the number of Escherichia coli O157:H7 and vegetative cells of Bacillus cereus in brining and beef during the curing processing. The mechanisms of the inactivation for the pathogens were preliminarily explored. The ultrasound intensity (2.39, 6.23, 11.32 and 20.96Wcm-2) and sonication time (30, 60, 90 and 120min) at 10°C were assessed in brine and meat. Plate count data indicated that 20.96Wcm-2 for 120min was the optimal treatment for bacterial reductions...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29193894/phenotypic-and-genotypic-comparison-of-salmonellae-from-diarrhoeic-and-healthy-humans-and-cattle-nigeria
#10
K Fashae, P Leekitcharoenphon, R S Hendriksen
The sources and modes of transmission of non-typhoidal Salmonella particularly zoonotic transmission are poorly understood in Africa. This study compared phenotypic and genotypic characteristics of Salmonellae isolated from cattle and humans. Faecal samples of diarrhoeic patients (n = 234), and a healthy population (n = 160), beef cattle at slaughter (n = 250), farms (n = 72) and market (n = 100) were cultured for salmonellae and serotyping and antimicrobial susceptibility were determined. Whole-genome sequence typing (WGST) of selected isolates and bioinformatic analysis were used to identify the multilocus sequence type (MLST), plasmid replicons, antimicrobial resistance genes and genetic relatedness by single nucleotide polymorphism (SNP) analysis...
November 28, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/29191491/a-nested-pcr-strategy-for-molecular-diagnosis-of-mollicutes-in-uncultured-biological-samples-from-cows-with-vulvovaginitis
#11
Daniele Cristina Voltarelli, Brígida Kussumoto de Alcântara, Michele Lunardi, Alice Fernandes Alfieri, Raquel de Arruda Leme, Amauri Alcindo Alfieri
Bacteria classified in Mycoplasma (M. bovis and M. bovigenitalium) and Ureaplasma (U. diversum) genera are associated with granular vulvovaginitis that affect heifers and cows at reproductive age. The traditional means for detection and speciation of mollicutes from clinical samples have been culture and serology. However, challenges experienced with these laboratory methods have hampered assessment of their impact in pathogenesis and epidemiology in cattle worldwide. The aim of this study was to develop a PCR strategy to detect and primarily discriminate between the main species of mollicutes associated with reproductive disorders of cattle in uncultured clinical samples...
November 24, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29186410/induction-of-a-viable-but-non-culturable-state-in-salmonella-typhimurium-by-thermosonication-and-factors-affecting-resuscitation
#12
Hongmei Liao, Lifen Jiang, Ruirui Zhang
The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhimurium cells in BPY broth, apple/carrot juice, physiological saline and phosphate buffer solution. The logarithmic and decline phases of growth were more sensitive to the TS treatment compared to stationary phase cells...
November 27, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29155021/suitability-of-a-chocolate-agar-based-medium-for-the-transportation-and-long-term-storage-of-neisseria-gonorrhoeae-isolates-at-room-temperature
#13
Sidharath D Thakur, Jo-Anne R Dillon
Neisseria gonorrhoeae isolates were grown on chocolate agar slants comprising GC Agar Medium Base supplemented with 1% modified Kellogg's supplement and 1% beef blood hemoglobin. The bacterial growth was covered with sterilized paraffin oil. Cultures were used for transportation and storage of Neisseria gonorrhoeae for 3months at room temperature.
November 15, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29130766/molecular-characterization-of-salmonella-from-beef-carcasses-and-fecal-samples-from-an-integrated-feedlot-and-abattoir-in-mexico
#14
Diana Ayala, Kendra Nightingale, Claudia Narvaez-Bravo, Mindy M Brashears
Nontyphoid Salmonella strains are some of the leading causes of foodborne illnesses worldwide; however, there is very limited information on the presence and characteristics of Salmonella in the food production chain in developing countries. In this study, pulsed-field gel electrophoresis (PFGE) was used for molecular subtyping and for monitoring the ecology and transmission of Salmonella isolates in a slaughter facility in Mexico in an attempt to determine specific steps that need to be improved to reduce Salmonella contamination in beef carcasses...
December 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29119377/meat-retail-conditions-within-the-establishments-of-kigali-city-rwanda-bacteriological-quality-and-risk-factors-for-salmonella-occurrence
#15
Eugène Niyonzima, Martin Patrick Ongol, Yves Brostaux, Nicolas Korsak, Georges Daube, Anastase Kimonyo, Marianne Sindic
Meat constitutes one of the major vehicles for human foodborne infections. This study aimed to assess the retail conditions and to determine the microbiological quality and safety of meat retailed within the establishments of Kigali (Rwanda). A questionnaire survey was carried out in 150 retail outlets to characterise meat retail conditions. Additionally, 270 retail meat samples were analysed for the enumeration of hygiene indicator bacteria (total mesophilic bacteria and Escherichia coli) and for the qualitative detection of Salmonella, using conventional culture methods...
November 8, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29108068/rapid-communication-16s-ribosomal-ribonucleic-acid-characterization-of-liver-abscesses-in-feedlot-cattle-from-three-states-in-the-united-states
#16
M D Weinroth, C R Carlson, J N Martin, J L Metcalf, P S Morley, K E Belk
Liver abscesses are a major economic burden to beef producers. Although a few causative organisms have been cultured from purulent material, the full polymicrobial diversity of liver abscesses has not been reported. The objective of this study was to characterize purulent material collected from liver abscess in beef cattle produced in different production systems in 3 cattle producing states in the United States using 16S rRNA gene sequencing. Differences between purulent material microbial communities among geographic region of feeding and application of a common antimicrobial were also investigated...
October 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/29108044/consequences-of-exposure-of-embryos-produced-in-vitro-in-a-serum-containing-medium-to-dickkopf-related-protein-1-and-colony-stimulating-factor-2-on-blastocyst-yield-pregnancy-rate-and-birth-weight
#17
P Tríbulo, B H Bernal Ballesteros, A Ruiz, A Tríbulo, R J Tríbulo, H E Tríbulo, G A Bo, P J Hansen
Embryokines are molecules secreted by the mother that regulate embryonic development. Among these molecules in cattle are colony stimulating factor 2 (CSF2) and dickkopf-related protein 1 (DKK1). Here, we evaluated actions of CSF2 and DKK1 alone or in combination on characteristics of embryos produced in vitro in the presence of serum. A total of 70 beef cows from 4 farms were subjected to oocyte retrieval on 1 to 4 occasions. Within each farm, donors were randomly allocated to 1 of 4 treatment groups (vehicle, CSF2, DKK1, CSF2 + DKK1)...
October 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/29091044/dispersion-risk-associated-with-surgical-debridement-devices
#18
Mark Granick, Liel Rubinsky, Chaitra Parthiban, Mayilvahanan Shanmugam, Narayanan Ramasubbu
INTRODUCTION: Surgical instrumentation is now available to facilitate wound debridement. The 2 primary options involve different energy applications, but both have the potential to spray. The Versajet II (Smith & Nephew, London, UK) utilizes a high-powered water jet to disrupt tissue and remove debris by means of the Venturi effect. The SonicVac (Misonix, Farmingdale, NY) is a direct-contact, low-frequency ultrasound debriding device. It delivers a high-energy ultrasound to a wound surface via a fluid medium, causing bubble cavitation, a physical effect of rapid pressure waves causing bubbles to form and implode that releases mechanical energy...
October 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/29059725/bio-protective-potential-of-lactic-acid-bacteria-effect-of-lactobacillus-sakei-and-lactobacillus-curvatus-on-changes-of-the-microbial-community-in-vacuum-packaged-chilled-beef
#19
Yimin Zhang, Lixian Zhu, Pengcheng Dong, Rongrong Liang, Yanwei Mao, Shubing Qiu, Xin Luo
Objective: This study was to determine the bacterial diversity and monitor the community dynamic changes during storage of vacuum-packaged sliced raw beef as affected by Lactobacillus sakei and Lactobacillus curvatus. Methods: L. sakei and L. curvatus were separately incubated in vacuumed-packaged raw beef as bio-protective cultures to inhibit the naturally contaminating microbial load. Dynamic changes of the microbial diversity of inoculated or non-inoculated (control) samples were monitored at 4 °C for 0 to 38 days, using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)...
October 20, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/29023016/indigenous-halomonas-spp-the-potential-nitrifying-bacteria-for-saline-ammonium-waste-water-treatment
#20
Yutthapong Sangnoi, Sunipa Chankaew, Sompong O-Thong
BACKGROUND AND OBJECTIVE: Toxic nitrogen compounds are one cause decreasing of shrimp production and water pollution. Indigenous Halomonas spp., isolated from Pacific white shrimp farm are benefitted for saline ammonium waste water treatment. This study aimed to isolate the heterotrophic-halophilic Halomonas spp. and investigate their ammonium removal efficiency. MATERIALS AND METHODS: Halomonas spp., were isolated by culturing of samples collected from shrimp farm into modified Pep-Beef-AOM medium...
2017: Pakistan Journal of Biological Sciences: PJBS
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