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https://www.readbyqxmd.com/read/28544731/taking-control-over-microbial-populations-current-approaches-for-exploiting-biological-noise-in-bioprocesses
#1
REVIEW
Frank Delvigne, Jonathan Baert, Hosni Sassi, Patrick Fickers, Alexander Grünberger, Christian Dusny
Phenotypic plasticity of microbial cells has attracted much attention and several research efforts have been dedicated to the description of methods aiming at characterizing phenotypic heterogeneity and its impact on microbial populations. However, different approaches have also been suggested in order to take benefit from noise in a bioprocess perspective, e.g. by increasing the robustness or productivity of a microbial population. This review is dedicated to outline these controlling methods. A common issue, that has still to be addressed, is the experimental identification and the mathematical expression of noise...
May 24, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28535986/intracellular-cellobiose-metabolism-and-its-applications-in-lignocellulose-based-biorefineries
#2
REVIEW
Vinuselvi Parisutham, Sathesh-Prabu Chandran, Aindrila Mukhopadhyay, Sung Kuk Lee, Jay D Keasling
Complete hydrolysis of cellulose has been a key characteristic of biomass technology because of the limitation of industrial production hosts to use cellodextrin, the partial hydrolysis product of cellulose. Cellobiose, a β-1,4-linked glucose dimer, is a major cellodextrin of the enzymatic hydrolysis (via endoglucanase and exoglucanase) of cellulose. Conversion of cellobiose to glucose is executed by β-glucosidase. The complete extracellular hydrolysis of celluloses has several critical barriers in biomass technology...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28534676/applications-of-raman-spectroscopy-in-biopharmaceutical-manufacturing-a-short-review
#3
Kevin Buckley, Alan G Ryder
The production of active pharmaceutical ingredients (APIs) is currently undergoing its biggest transformation in a century. The changes are based on the rapid and dramatic introduction of protein- and macromolecule-based drugs (collectively known as biopharmaceuticals) and can be traced back to the huge investment in biomedical science (in particular in genomics and proteomics) that has been ongoing since the 1970s. Biopharmaceuticals (or biologics) are manufactured using biological-expression systems (such as mammalian, bacterial, insect cells, etc...
June 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28533816/conferring-cellulose-degrading-ability-to-yarrowia-lipolytica-to-facilitate-a-consolidated-bioprocessing-approach
#4
Zhong-Peng Guo, Sophie Duquesne, Sophie Bozonnet, Gianluca Cioci, Jean-Marc Nicaud, Alain Marty, Michael Joseph O'Donohue
BACKGROUND: Yarrowia lipolytica, one of the most widely studied "nonconventional" oleaginous yeast species, is unable to grow on cellulose. Recently, we identified and overexpressed two endogenous β-glucosidases in Y. lipolytica, thus enabling this yeast to use cello-oligosaccharides as a carbon source for growth. Using this engineered yeast platform, we have now gone further toward building a fully cellulolytic Y. lipolytica for use in consolidated bioprocessing of cellulose. RESULTS: Initially, different essential enzyme components of a cellulase cocktail (i...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28531862/unravelling-the-suitability-of-biological-induction-for-halophilic-lipase-production-by-halomonas-sp-lm1c-cultures
#5
Esther Gutiérrez-Arnillas, María Arellano, Francisco J Deive, Ana Rodríguez, María Ángeles Sanromán
In this study, the viability of using biological induction as an alternative to the conventional chemical induction in lipase production by a novel halophilic microorganism, Halomonas sp. LM1C, has been demonstrated. Thus, a 9-times increase of lipase activity (3000U/L) was recorded when Staphylococcus equorum sp. AMC7 was present in the medium, which is competitive with the results obtained when Triton X-100 was added as chemical inducer. The GC-MS data allowed concluding the true nature of the biological inducer effect, as the existence of high percentages of isomeric forms of pentadecanoic acid were detected...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28529041/treatment-of-supermarket-vegetable-wastes-to-be-used-as-alternative-substrates-in-bioprocesses
#6
Ana Isabel Díaz, Amanda Laca, Adriana Laca, Mario Díaz
Fruits and vegetables have the highest wastage rates at retail and consumer levels. These wastes have promising potential for being used as substrates in bioprocesses. However, an effective hydrolysis of carbohydrates that form these residues has to be developed before the biotransformation. In this work, vegetable wastes from supermarket (tomatoes, green peppers and potatoes) have been separately treated by acid, thermal and enzymatic hydrolysis processes in order to maximise the concentration of fermentable sugars in the final broth...
May 18, 2017: Waste Management
https://www.readbyqxmd.com/read/28528952/an-overview-of-natural-renewable-bio-polymer-lignin-towards-nano-and-biotechnological-applications
#7
REVIEW
Selvaraj Mohana Roopan
Lignin were said to be major bio-polymer next to cellulose which is an abundant biopolymer. This type of lignin was mostly isolated from woods which were named after their physical, morphological appearances and majorly on extracting sources. Still now there are very few reports on isolation, identification of pure lignin and isolating pathways are also not well defined. Molecular weight of lignin varies from thousands to ten thousands which are not explored accurately. Even-though lignins were surrounded by these many hurdles it has various application studies which were studied and reported...
May 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28528086/cellulosic-ethanol-status-and-innovation
#8
REVIEW
Lee R Lynd, Xiaoyu Liang, Mary J Biddy, Andrew Allee, Hao Cai, Thomas Foust, Michael E Himmel, Mark S Laser, Michael Wang, Charles E Wyman
Although the purchase price of cellulosic feedstocks is competitive with petroleum on an energy basis, the cost of lignocellulose conversion to ethanol using today's technology is high. Cost reductions can be pursued via either in-paradigm or new-paradigm innovation. As an example of new-paradigm innovation, consolidated bioprocessing using thermophilic bacteria combined with milling during fermentation (cotreatment) is analyzed. Acknowledging the nascent state of this approach, our analysis indicates potential for radically improved cost competitiveness and feasibility at smaller scale compared to current technology, arising from (a) R&D-driven advances (consolidated bioprocessing with cotreatment in lieu of thermochemical pretreatment and added fungal cellulase), and (b) configurational changes (fuel pellet coproduction instead of electricity, gas boiler(s) in lieu of a solid fuel boiler)...
May 18, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28524856/histone-variant-h3-3-orchestrates-neural-stem-cell-differentiation-in-the-developing-brain
#9
Wenlong Xia, Jianwei Jiao
During the brain development, the process of neural stem cells (NSCs) proliferation and differentiation is precisely regulated. The deficiency in the embryonic brain development will cause serious developmental disorders. Epigenetic modifications play critical roles in controlling proliferation and differentiation in different types of stem cells. Histone variants, as one of epigenetic regulators, have been reported to be associated with many bioprocesses. Among different variants, H3.3 is one of the important epigenetic regulators, but its role in embryonic NSCs remains unclear...
May 19, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28518086/ptr-tof-ms-coupled-with-an-automated-sampling-system-and-tailored-data-analysis-for-food-studies-bioprocess-monitoring-screening-and-nose-space-analysis
#10
Vittorio Capozzi, Sine Yener, Iuliia Khomenko, Brian Farneti, Luca Cappellin, Flavia Gasperi, Matteo Scampicchio, Franco Biasioli
Proton Transfer Reaction (PTR), combined with a Time-of-Flight (ToF) Mass Spectrometer (MS) is an analytical approach based on chemical ionization that belongs to the Direct-Injection Mass Spectrometric (DIMS) technologies. These techniques allow the rapid determination of volatile organic compounds (VOCs), assuring high sensitivity and accuracy. In general, PTR-MS requires neither sample preparation nor sample destruction, allowing real time and non-invasive analysis of samples. PTR-MS are exploited in many fields, from environmental and atmospheric chemistry to medical and biological sciences...
May 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28514082/universal-label-free-in-process-quantification-of-influenza-virus-like-particles
#11
Sofia B Carvalho, Mafalda G Moleirinho, David Wheatley, John Welsh, René Gantier, Paula M Alves, Cristina Peixoto, Manuel J T Carrondo
Virus-like particles (VLPs) are becoming established as vaccines, in particular for influenza pandemics, increasing the interest in the development of VLPs manufacturing bioprocess. However, for complex VLPs, the analytical tools used for quantification are not yet able to keep up with the bioprocess progress. Currently, quantification for Influenza relies on traditional methods: hemagglutination assay or Single Radial Immunodiffusion. These analytical technologies are time-consuming, cumbersome, and not supportive of efficient downstream process development and monitoring...
May 17, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28511586/bioprocess-optimization-of-phb-homopolymer-and-copolymer-p3-hb-co-hv-by-acinetobacter-junii-bp25-utilizing-rice-mill-effluent-as-sustainable-substrate
#12
S Poorna Chandrika, D Sabarinathan, P Anburajan, K Preethi
The potential use of parboiled rice mill effluent as a cheap substrate for the production of homopolymer and copolymer of Polyhydroxyalkanoates (PHAs) by Acinetobacter junii BP 25 was investigated for the first time. Process optimization by one factor at a time led to homopolymer polyhydroxybutyrate (PHB) production of 2.64 ± 0.18 g/l with 94.28% PHB content using a two stage batch cultivation mode. BP 25 furthermore produced polyhydroxybutyrate-co-hydroxyvalerate (P3 (HB-co-HV)), with the addition of valeric acid as an additive to the substrate, yielding (2...
May 16, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28506668/characterization-of-selected-municipal-solid-waste-components-to-estimate-their-biodegradability
#13
R Bayard, H Benbelkacem, R Gourdon, P Buffière
Biological treatments of Residual Municipal Solid Waste (RMSW) allow to divert biodegradable materials from landfilling and recover valuable alternative resources. The biodegradability of the waste components needs however to be assessed in order to design the bioprocesses properly. The present study investigated complementary approaches to aerobic and anaerobic biotests for a more rapid evaluation. A representative sample of residual MSW was collected from a Mechanical Biological Treatment (MBT) plant and sorted out into 13 fractions according to the French standard procedure MODECOM™...
May 12, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28504349/polysome-profiling-of-mab-producing-cho-cell-lines-links-translational-control-of-cell-proliferation-and-recombinant-mrna-loading-onto-ribosomes-with-global-and-recombinant-protein-synthesis
#14
Charlotte L Godfrey, Emma J Mead, Olalekan Daramola, Sarah Dunn, Diane Hatton, Ray Field, Gary Pettman, C Mark Smales
mRNA translation is a key process determining growth, proliferation and duration of a Chinese hamster ovary (CHO) cell culture and influences recombinant protein synthesis rate. During bioprocessing, CHO cells can experience stresses leading to reprogramming of translation and decreased global protein synthesis. Here we apply polysome profiling to determine reprogramming and translational capabilities in host and recombinant monoclonal antibody-producing (mAb) CHO cell lines during batch culture. Recombinant cell lines with the fastest cell specific growth rates were those with the highest global translational efficiency...
May 15, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28503195/strategies-for-improved-isopropanol-butanol-production-by-a-clostridium-strain-from-glucose-and-hemicellulose-through-consolidated-bioprocessing
#15
Fengxue Xin, Tianpeng Chen, Yujiang Jiang, Weiliang Dong, Wenming Zhang, Min Zhang, Hao Wu, Jiangfeng Ma, Min Jiang
BACKGROUND: High cost of traditional substrates and formation of by-products (such as acetone and ethanol) in acetone-butanol-ethanol (ABE) fermentation hindered the large-scale production of biobutanol. Here, we comprehensively characterized a newly isolated solventogenic and xylanolytic Clostridium species, which could produce butanol at a high ratio with elimination of ethanol and conversion of acetone to more value-added product, isopropanol. Ultimately, direct butanol production from hemicellulose was achieved with efficient expression of indigenous xylanase by the novel strain via consolidated bioprocessing...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28499817/use-of-cellnetanalyzer-in-biotechnology-and-metabolic-engineering
#16
Axel von Kamp, Sven Thiele, Oliver Hädicke, Steffen Klamt
Mathematical models of the cellular metabolism have become an essential tool for the optimization of biotechnological processes. They help to obtain a systemic understanding of the metabolic processes in the used microorganisms and to find suitable genetic modifications maximizing the production performance. In particular, methods of stoichiometric and constraint-based modeling are frequently used in the context of metabolic and bioprocess engineering. Since metabolic networks can be complex and comprise hundreds or even thousands of metabolites and reactions, dedicated software tools are required for an efficient analysis...
May 9, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28498115/field-campaign-on-sediment-transport-behaviour-in-a-pressure-main-from-pumping-station-to-wastewater-treatment-plant-in-berlin
#17
M Gunkel, E Pawlowsky-Reusing
As part of the project KURAS, the Berliner Wasserbetriebe realized a field campaign in 2015 in order to increase the process knowledge regarding the behaviour of transported sediment in the pressure main leading from the pumpstation to the wastewater treatment plant. The field campaign was conducted because of a lack of knowledge about the general condition of the pressure main due to its bad accessibility and the suspicion of deposits caused by hydraulic underload. The practical evidence of the sediment transport performance of this part of the sewer system, dependent on different load cases, should present a basis for further analysis, for example regarding flushing measures...
May 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28497711/production-and-covalent-immobilisation-of-the-recombinant-bacterial-carbonic-anhydrase-sspca-onto-magnetic-nanoparticles
#18
Rosa Perfetto, Sonia Del Prete, Daniela Vullo, Giovanni Sansone, Carmela M A Barone, Mosè Rossi, Claudiu T Supuran, Clemente Capasso
Carbonic anhydrases (CAs; EC 4.2.1.1) are metalloenzymes with a pivotal potential role in the biomimetic CO2 capture process (CCP) because these biocatalysts catalyse the simple but physiologically crucial reaction of carbon dioxide hydration to bicarbonate and protons in all life kingdoms. The CAs are among the fastest known enzymes, with kcat values of up to 10(6) s(-1) for some members of the superfamily, providing thus advantages when compared with other CCP methods, as they are specific for CO2. Thermostable CAs might be used in CCP technology because of their ability to perform catalysis in operatively hard conditions, typical of the industrial processes...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28497573/simplex-based-optimization-of-numerical-and-categorical-inputs-in-early-bioprocess-development-case-studies-in-ht-chromatography
#19
Spyridon Konstantinidis, Nigel Titchener-Hooker, Ajoy Velayudhan
Bioprocess development studies often involve the investigation of numerical and categorical inputs via the adoption of Design of Experiments (DoE) techniques. An attractive alternative is the deployment of a grid compatible Simplex variant which has been shown to yield optima rapidly and consistently. In this work, the method is combined with dummy variables and it is deployed in three case studies wherein spaces are comprised of both categorical and numerical inputs, a situation intractable by traditional Simplex methods...
May 12, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28497290/medium-chain-length-polyhydroxyalkanoates-synthesis-by-pseudomonas-putida-kt2440-rela-spot-mutant-bioprocess-characterization-and-transcriptome-analysis
#20
Justyna Mozejko-Ciesielska, Dorota Dabrowska, Agnieszka Szalewska-Palasz, Slawomir Ciesielski
Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli are still not clear. The rearrangement of the metabolism in response to environmental stress could be controlled by stringent response that modulates the transcription of many genes in order to promote survival under nutritional deprivation conditions...
December 2017: AMB Express
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