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https://www.readbyqxmd.com/read/28441870/protein-nanocellulose-interactions-in-paper-filters-for-advanced-separation-applications
#1
Simon Johan Gustafsson, Levon Manukyan, Albert Mihranyan
Protein-based pharmaceutics are widely explored for healthcare applications, and six out of ten best selling drugs today are biologicals. The goal of this work was to evaluate the protein nanocellulose interactions in paper filter for advanced separation applications such as virus removal filtration and bioprocessing. The protein recovery was measured for bovine serum albumin (BSA), γ-Globulin and Lysozyme using biuret total protein reagent and polyacrylamide gel electrophoresis (SDS-PAGE), and the throughput was characterized in terms of flux values from fixed volume filtrations at various protein concentrations and under worst-case experimental conditions...
April 26, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28437742/biological-treatment-of-model-dyes-and-textile-wastewaters
#2
Alicia Paz, Julia Carballo, María José Pérez, José Manuel Domínguez
Previous works conducted in our laboratory, reveled that Bacillus aryabhattai DC100 produce ligninolytic enzymes such as laccases and/or peroxidases, opening new applications in different bioprocesses, including the treatment of disposal residues such as dyestuffs from textile processing industries. This work described the degradation of three commercial model dyes Coomassie Brilliant Blue G-250 (CBB), Indigo Carmine (IC) and Remazol Brilliant Blue R (RBBR) under different culture media and operational conditions...
April 18, 2017: Chemosphere
https://www.readbyqxmd.com/read/28436005/fast-and-reliable-strain-characterization-of-streptomyces-lividans-through-micro-scale-cultivation
#3
Joachim Koepff, Matthias Keller, C Konstantinos Tsolis, Tobias Busche, Christian Rückert, Mohamed B Hamed, Jozef Anné, Joern Kalinowski, Wolfgang Wiechert, Anastassios Economou, Marco Oldiges
Filamentous organisms of the genus Streptomyces play an important role in industrial production processes, due to their extensive secondary metabolism variability, as well as their ability to secrete efficiently large amounts of (heterologous) proteins. While genetic engineering tools are available to rapidly build up large strain libraries, the subsequent strain screening and bioprocess development still constitutes a bottleneck. This is due to the lack of reliable parallelized and accelerated cultivation techniques for morphologically challenging organisms...
April 24, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28423945/on-line-soft-sensing-in-upstream-bioprocessing
#4
Judit Randek, Carl-Fredrik Mandenius
This review provides an overview and a critical discussion of novel possibilities of applying soft sensors for on-line monitoring and control of industrial bioprocesses. Focus is on bio-product formation in the upstream process but also the integration with other parts of the process is addressed. The term soft sensor is used for the combination of analytical hardware data (from sensors, analytical devices, instruments and actuators) with mathematical models that create new real-time information about the process...
April 20, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28409424/enhanced-saccharification-of-lignocellulosic-agricultural-biomass-and-increased-bioethanol-titre-using-acclimated-clostridium-thermocellum-dsm1313
#5
M Nisha, K Saranyah, Mukund Shankar, L M Saleena
Consolidated bioprocess assures an efficient lignocellulosic conversion to fermentable sugars and subsequently to bioethanol. Such a single-step hydrolysis and anaerobic fermentation was achieved with acclimated Clostridium thermocellum DSM 1313 on different mildly pre-treated agricultural lignocellulosic residues without any additional enzymes/and strains. Acclimation was achieved by serially sub-culturing in increasing concentration of individual substrates, such as rice husk, sugarcane bagasse, and banana pseudostem in the standard media, with cellobiose as an adjunct...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28405217/bioprocessing-of-tea-oil-fruit-hull-with-acetic-acid-organosolv-pretreatment-in-combination-with-alkaline-h2o2
#6
Song Tang, Rukuan Liu, Fubao Fuelbiol Sun, Chunying Dong, Rui Wang, Zhongyuan Gao, Zhanying Zhang, Zhihong Xiao, Changzhu Li, Hui Li
BACKGROUND: As a natural renewable biomass, the tea oil fruit hull (TOFH) mainly consists of lignocellulose, together with some bioactive substances. Our earlier work constructed a two-stage solvent-based process, including one aqueous ethanol organosolv extraction and an atmospheric glycerol organosolv (AGO) pretreatment, for bioprocessing of the TOFH into diverse bioproducts. However, the AGO pretreatment is not as selective as expected in removing the lignin from TOFH, resulting in the limited delignification and simultaneously high cellulose loss...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28400329/deletion-of-type-i-glutamine-synthetase-deregulates-nitrogen-metabolism-and-increases-ethanol-production-in-clostridium-thermocellum
#7
Thomas Rydzak, David Garcia, David M Stevenson, Margaret Sladek, Dawn M Klingeman, Evert K Holwerda, Daniel Amador-Noguez, Steven D Brown, Adam M Guss
Clostridium thermocellum rapidly deconstructs cellulose and ferments resulting hydrolysis products into ethanol and other products, and is thus a promising platform organism for the development of cellulosic biofuel production via consolidated bioprocessing. While recent metabolic engineering strategies have targeted eliminating canonical fermentation products (acetate, lactate, formate, and H2), C. thermocellum also secretes amino acids, which has limited ethanol yields in engineered strains to approximately 70% of the theoretical maximum...
April 8, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28400170/enhanced-simultaneous-saccharification-and-fermentation-of-pretreated-beech-wood-by-in-situ-treatment-with-the-white-rot-fungus-irpex-lacteus-in-a-membrane-aerated-biofilm-reactor
#8
Simone Brethauer, Shahab Robert Lawrence, Studer Michael Hans-Peter
The aim of the present study was to investigate the combination of steam pretreatment and biological treatment with lignin degrading fungal strains in order to enable efficient bioprocessing of beech wood to ethanol. In a sequential process of steam and fungal pretreatment followed by enzymatic hydrolysis, Irpex lacteus almost doubled the glucose yield for mildly pretreated beech wood, but could not improve yields for more severely pretreated substrates. However, when simultaneous saccharification and fermentation is combined with in situ I...
March 18, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28400137/tools-for-the-determination-of-population-heterogeneity-caused-by-inhomogeneous-cultivation-conditions
#9
REVIEW
Anja Lemoine, Frank Delvigne, Anika Bockisch, Peter Neubauer, Stefan Junne
Population heterogeneity among a microbial culture can occur in various stages of a bioprocess. When a nutrient-limited fed-batch process is applied in the large scale, gradient formation (e.g. at the substrate concentration or the pH-value) can have a tremendous impact on the process performance. It might also have an impact on population heterogeneity, as the cells are opposed to stressful conditions that is an oscillating environment, when they pass the different zones evolved in the liquid phase. Nevertheless, the question whether these conditions support heterogeneity of a culture has not been clearly answered so far...
April 8, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28399663/upstream-biomanufacturing-of-pharmaceutical-colchicine
#10
Ganapathy Sivakumar
The plant-based colchicine potentially affects major diseases such as cardiovascular events, cancers and gout. Gloriosa superba seeds are a conventional pharmaceutical source of colchicine. The demand for pharmaceutical-grade colchicine has increased due to the shortage of feasible upstream manufacturing, encompassing all stages in the processes of biosynthesis and biomanufacturing before the raw material is ready for purification. Consequently, developing sustainable upstream industrial colchicine biofactories is imperative, especially in curtailing drug costs...
April 12, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28391735/diet-derived-changes-by-sourdough-fermented-rye-bread-in-exhaled-breath-aspiration-ion-mobility-spectrometry-profiles-in-individuals-with-mild-gastrointestinal-symptoms
#11
Kaisa Raninen, Jenni Lappi, Mikko Kolehmainen, Marjukka Kolehmainen, Hannu Mykkänen, Kaisa Poutanen, Olavi Raatikainen
The potential of utilising exhaled breath volatile organic compound (VOC) profiles in studying diet-derived metabolic changes was examined. After a four-week initial diet period with white wheat bread (WW), seven participants received in randomised order high-fibre diets containing sourdough whole grain rye bread (WGR) or white wheat bread enriched with bioprocessed rye bran (WW + BRB), both for 4 weeks. Alveolar exhaled breath samples were analysed with ChemPro(®)100i analyser (Environics OY, Mikkeli, Finland) at the end of each diet period in fasting state and after a standardised meal...
April 9, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28385593/a-genome-scale-model-of-geobacillus-thermoglucosidasius-c56-ys93-reveals-its-biotechnological-potential-on-rice-straw-hydrolysate
#12
Ahmad Ahmad, Hassan B Hartman, S Krishnakumar, David A Fell, Mark G Poolman, Shireesh Srivastava
Rice straw is a major crop residue which is burnt in many countries, creating significant air pollution. Thus, alternative routes for disposal of rice straw are needed. Biotechnological treatment of rice straw hydrolysate has potential to convert this agriculture waste into valuable biofuel(s) and platform chemicals. Geobacillus thermoglucosidasius is a thermophile with properties specially suited for use as a biocatalyst in lignocellulosic bioprocesses, such as high optimal temperature and tolerance to high levels of ethanol...
April 4, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28373050/microbial-based-synthesis-of-highly-elastomeric-biodegradable-poly-3-hydroxybutyrate-co-4-hydroxybutyrate-thermoplastic
#13
Kai-Hee Huong, Chin-Hoe Teh, A A Amirul
This study reports the production of P(3HB-co-4HB) [Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)] in possession of high molecular weight and elastomeric properties by Cupriavidus sp. USMAA1020 in single-stage mixed-substrate cultivation system. 1,4-butanediol and 1,6-hexanediol are found to be efficient substrate mixture that has resulted in high copolymer yield, occupying a maximum of 70wt% of the total biomass and producing higher 4HB monomer composition ranging from 31mol% to 41mol%. In substrate mixtures involving 1,6-hexanediol, cleavage of the 6-hydroxyhexanoyl-CoA produces Acetyl-CoA and 4-hydroxybutyryl-CoA...
April 1, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28371718/comparability-study-of-rituximab-originator-and-follow-on-biopharmaceutical
#14
Othman Montacir, Houda Montacir, Murat Eravci, Andreas Springer, Stephan Hinderlich, Amirhossein Saadati, Maria Kristina Parr
Immunglobolin G (IgG)-based biopharmaceuticals are emerging on the pharmaceuticals market due to their high target selectivity in different diseases. In parallel, a growing interest by other companies to produce similar or highly similar follow-on biologics exits, once the patent of blockbuster biotherapeutics is about to expire. In correlation to their complex structure, an analytical challenge is facing the approval of these biosimilars. Health authorities (e.g. FDA and EMA) have issued several guidelines to define critical quality attributes during manufacturing process changes...
June 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28371651/metals-and-minerals-as-a-biotechnology-feedstock-engineering-biomining-microbiology-for-bioenergy-applications
#15
REVIEW
Indrani Banerjee, Brittany Burrell, Cara Reed, Alan C West, Scott Banta
Developing new feedstocks for the efficient production of biochemicals and biofuels will be a critical challenge as we diversify away from petrochemicals. One possible opportunity is the utilization of sulfide-based minerals in the Earth's crust. Non-photosynthetic chemolithoautotrophic bacteria are starting to be developed to produce biochemicals from CO2 using energy obtained from the oxidation of inorganic feedstocks. Biomining of metals like gold and copper already exploit the native metabolism of these bacteria and these represent perhaps the largest-scale bioprocesses ever developed...
March 31, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28371494/quality-by-control-towards-model-predictive-control-of-mammalian-cell-culture-bioprocesses
#16
REVIEW
Wolfgang Sommeregger, Bernhard Sissolak, Kulwant Kandra, Moritz von Stosch, Martin Mayer, Gerald Striedner
The industrial production of complex biopharmaceuticals using recombinant mammalian cell lines is still mainly built on a quality by testing approach, which is represented by fixed process conditions and extensive testing of the end-product. In 2004 the FDA launched the process analytical technology initiative, aiming to guide the industry towards advanced process monitoring and better understanding of how critical process parameters affect the critical quality attributes. Implementation of process analytical technology into the bio-production process enables moving from the quality by testing to a more flexible quality by design approach...
March 31, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28367228/upgrading-syngas-fermentation-effluent-using-clostridium-kluyveri-in-a-continuous-fermentation
#17
Sylvia Gildemyn, Bastian Molitor, Joseph G Usack, Mytien Nguyen, Korneel Rabaey, Largus T Angenent
BACKGROUND: The product of current syngas fermentation systems is an ethanol/acetic acid mixture and the goal is to maximize ethanol recovery. However, ethanol currently has a relatively low market value and its separation from the fermentation broth is energy intensive. We can circumvent these disadvantages of ethanol production by converting the dilute ethanol/acetic acid mixture into products with longer carbon backbones, which are of higher value and are more easily extracted than ethanol...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28366567/centrifugal-partition-chromatography-in-a-biorefinery-context-optimisation-and-scale-up-of-monosaccharide-fractionation-from-hydrolysed-sugar-beet-pulp
#18
David P Ward, Peter Hewitson, Max Cárdenas-Fernández, Charlotte Hamley-Bennett, Alba Díaz-Rodríguez, Nathalie Douillet, Joseph P Adams, David J Leak, Svetlana Ignatova, Gary J Lye
The isolation of component sugars from biomass represents an important step in the bioprocessing of sustainable feedstocks such as sugar beet pulp. Centrifugal partition chromatography (CPC) is used here, as an alternative to multiple resin chromatography steps, to fractionate component monosaccharides from crude hydrolysed sugar beet pulp pectin. CPC separation of samples, prepared in the stationary phase, was carried out using an ethanol: ammonium sulphate (300gL(-1)) phase system (0.8:1.8v:v) in ascending mode...
March 6, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28365988/improvement-of-squalene-production-from-co2-in-synechococcus-elongatus-pcc-7942-by-metabolic-engineering-and-scalable-production-in-a-photobioreactor
#19
Sun Young Choi, Jin-Young Wang, Ho Seok Kwak, Sun-Mi Lee, Youngsoon Um, Yunje Kim, Sang Jun Sim, Jong-Il Choi, Han Min Woo
The push-and-pull strategy for metabolic engineering was successfully demonstrated in Synechococcus elongatus PCC 7942, a model photosynthetic bacterium, to produce squalene from CO2. Squalene synthase (SQS) was fused to either a key enzyme (farnesyl diphosphate synthase) of the methylerythritol phosphate pathway or the β-subunit of phycocyanin (CpcB1). Engineered cyanobacteria with expression of a fusion CpcB1-SQS protein showed a squalene production level (7.16 ± 0.05 mg/L/OD730) that was increased by 1...
April 3, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28365342/a-continuous-system-for-biocatalytic-hydrogenation-of-co2-to-formate
#20
Cláudia Mourato, Mónica Martins, Sofia M da Silva, Inês A C Pereira
In this work a novel bioprocess for hydrogenation of CO2 to formate was developed, using whole cell catalysis by a sulfate-reducing bacterium. Three Desulfovibrio species were tested (D. vulgaris Hildenborough, D. alaskensis G20, and D. desulfuricans ATCC 27774), of which D. desulfuricans showed the highest activity, producing 12mM of formate in batch, with a production rate of 0.09mMh(-1). Gene expression analysis indicated that among the three formate dehydrogenases and five hydrogenases, the cytoplasmic FdhAB and the periplasmic [FeFe] HydAB are the main enzymes expressed in D...
March 20, 2017: Bioresource Technology
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