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https://www.readbyqxmd.com/read/29345271/flocculation-on-a-chip-a-novel-screening-approach-to-determine-floc-growth-rates-and-select-flocculating-agents
#1
Anand N P Radhakrishnan, Marco P C Marques, Matthew J Davies, Brian O'Sullivan, Daniel G Bracewell, Nicolas Szita
Flocculation is a key purification step in cell-based processes for the food and pharmaceutical industry where the removal of cells and cellular debris is aided by adding flocculating agents. However, finding the best suited flocculating agent and optimal conditions to achieve rapid and effective flocculation is a non-trivial task. In conventional analytical systems, turbulent mixing creates a dynamic equilibrium between floc growth and breakage, constraining the determination of floc formation rates. Furthermore, these systems typically rely on end-point measurements only...
January 18, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29341493/modeling-the-downstream-processing-of-monoclonal-antibodies-reveals-cost-advantages-for-continuous-methods-for-a-broad-range-of-manufacturing-scales
#2
Jonathan Hummel, Mark Pagkaliwangan, Xhorxhi Gjoka, Terence Davidovits, Rick Stock, Thomas Ransohoff, Rene Gantier, Mark Schofield
The biopharmaceutical industry is evolving in response to changing market conditions, including increasing competition and growing pressures to reduce costs. Single-use technologies and continuous bioprocessing have attracted attention as potential facilitators of cost-optimized manufacturing for monoclonal antibodies. While disposable bioprocessing has been adopted at many scales of manufacturing, continuous bioprocessing has yet to reach the same level of implementation. In this study, the cost of goods of Pall Life Science's integrated, continuous bioprocessing platform was modeled, along with that of purification processes in stainless-steel and single-use batch formats...
January 17, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29341427/lc-ms-based-intact-igg-and-released-glycan-analysis-for-bioprocessing-applications
#3
Tianhua Wang, Kong Meng Hoi, Henning Stöckmann, Corrine Wan, Lyn Chiin Sim, Noor Hayati Bte Kamari, Shi Jie Tay, Ce Huang Poo, Susanto Woen, Yuangsheng Yang, Peiqing Zhang, Pauline M Rudd
Robust plate based antibody glycan analysis platforms are urgently needed for biopharmaceutical development and manufacturing as well as for clinical biomarker research. A 96-well plate based workflow has been developed to analyze both intact IgG antibodies and released N-glycans using an Orbitrap Fusion Mass Spectrometer and an LC/MS method on the Waters UNIFI platform. Here we describe such a workflow including protein A purification, PNGaseF digestion, 2-AB labeling and SPE clean-up. The measured IgG glycan profile is consistent with that obtained from non-plate based method and commercial kit and has the advantage of less hands-on time...
January 17, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29339118/aerobic-and-anaerobic-cellulose-utilization-by-paenibacillus-sp-caa11-and-enhancement-of-its-cellulolytic-ability-by-expressing-a-heterologous-endoglucanase
#4
Eun Sook Kim, Byeong-Soo Kim, Ki-Yeon Kim, Han-Min Woo, Sun-Mi Lee, Youngsoon Um
For cost-effective lignocellulosic biofuel/chemical production, consolidated bioprocessing (CBP)-enabling microorganisms utilizing cellulose as well as producing biofuel/chemical are required. A novel strain Paenibacillus sp. CAA11 isolated from sediment was found to be not only as a cellulose degrader under both aerobic and strict anaerobic conditions but also as a producer of cellulosic biofuel/chemicals. Paenibacillus sp. CAA11 secreted cellulolytic enzymes by its own secretion system and produced ethanol as well as short-chain organic acids (formic acid, acetic acid, lactic acid) from cellulose...
January 12, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29331827/pilot-scale-outdoor-photobioreactor-culture-of-the-marine-dinoflagellate-karlodinium-veneficum-production-of-a-karlotoxins-rich-extract
#5
L López-Rosales, A Sánchez-Mirón, F García-Camacho, A R Place, Yusuf Chisti, E Molina-Grima
A pilot-scale bioprocess was developed for the production of karlotoxin-enriched extracts of the marine algal dinoflagellate Karlodinium veneficum. A bubble column and a flat-panel photobioreactors (80-281 L) were used for comparative assessment of growth. Flow hydrodynamics and energy dissipation rates (EDR) in the bioreactors were characterized through robust computational fluid dynamic simulations. All cultures were conducted monoseptically outdoors. Bubble column (maximum cell productivity in semicontinuous operation of 58 × 103 cell mL-1 day-1) proved to be a better culture system for this alga...
December 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29330927/defined-serum-free-medium-for-bioreactor-culture-of-an-immortalized-human-erythroblast-cell-line
#6
Esmond Lee, Zhong Ri Lim, Hong Yu-Chen, Bin Xia Yang, Alan Tin-Lun Lam, Allen Kuan-Liang Chen, Jaichandran Sivalingam, Shaul Reuveny, Yuin-Han Loh, Steve Kah-Weng Oh
Anticipated shortages in donated blood supply have prompted investigation of alternative approaches for in-vitro production of red blood cells (RBCs), such as expansion of conditional immortalization erythroid progenitors. However, there is a bioprocessing challenge wherein factors promoting maximal cell expansion and growth-limiting inhibitory factors are yet to be investigated. We used an erythroblast cell line (ImEry) derived from immortalizing CD71+CD235a+ erythroblast from adult peripheral blood for optimization of expansion culture conditions...
January 13, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29330710/glycogen-production-in-marine-cyanobacterial-strain-synechococcus-sp-nkbg-15041c
#7
Amr Badary, Shouhei Takamatsu, Mitsuharu Nakajima, Stefano Ferri, Peter Lindblad, Koji Sode
An important feature offered by marine cyanobacterial strains over freshwater strains is the capacity to grow in seawater, replacing the need for often-limited freshwater. However, there are only limited numbers of marine cyanobacteria that are available for genetic manipulation and bioprocess applications. The marine unicellular cyanobacteria Synechococcus sp. strain NKBG 15041c (NKBG15041c) has been extensively studied. Recombinant DNA technologies are available for this strain, and its genomic information has been elucidated...
January 12, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29330649/growth-of-leptospirillum-ferriphilum-in-sulfur-medium-in-co-culture-with-acidithiobacillus-caldus
#8
Sarah L Smith, D Barrie Johnson
Leptospirillum ferriphilum and Acidithiobacillus caldus are both thermotolerant acidophilic bacteria that frequently co-exist in natural and man-made environments, such as biomining sites. Both are aerobic chemolithotrophs; L. ferriphilum is known only to use ferrous iron as electron donor, while A. caldus can use zero-valent and reduced sulfur, and also hydrogen, as electron donors. It has recently been demonstrated that A. caldus reduces ferric iron to ferrous when grown aerobically on sulfur. Experiments were carried out which demonstrated that this allowed L...
January 12, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29329775/recent-advances-and-strategies-in-process-and-strain-engineering-for-the-production-of-butyric-acid-by-microbial-fermentation
#9
REVIEW
Hongzhen Luo, Rongling Yang, Yuping Zhao, Zhaoyu Wang, Zheng Liu, Mengyu Huang, Qingwei Zeng
Butyric acid is an important platform chemical, which is widely used in the fields of food, pharmaceutical, energy, etc. Microbial fermentation as an alternative approach for butyric acid production is attracting great attention as it is an environmentally friendly bioprocessing. However, traditional fermentative butyric acid production is still not economically competitive compared to chemical synthesis route, due to the low titer, low productivity, and high production cost. Therefore, reduction of butyric acid production cost by utilization of alternative inexpensive feedstock, and improvement of butyric acid production and productivity has become an important target...
January 3, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29327394/photothermal-responsive-conjugated-polymer-nanoparticles-for-remote-control-of-gene-expression-in-living-cells
#10
Yunxia Wang, Shengliang Li, Pengbo Zhang, Haotian Bai, Liheng Feng, Fengting Lv, Libing Liu, Shu Wang
Remote control and noninvasive manipulation of cellular bioprocess has received intensive attention as a powerful technology to control cell functions. Here, a strategy is developed to remotely control intracellular gene expression with high spatial and temporal resolutions by using photothermal-responsive conjugated polymer nanoparticles (CPNs) as the transducer under near-infrared light irradiation. After being modified with positive charged peptide, the CPNs with superior photothermal conversion capacity could effectively coat on the surface of living cells and generate localized heat to trigger target gene expression...
January 12, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29321810/metabolic-engineering-of-pichia-pastoris-for-production-of-isobutanol-and-isobutyl-acetate
#11
Wiparat Siripong, Philipp Wolf, Theodora Puspowangi Kusumoputri, Joe James Downes, Kanokarn Kocharin, Sutipa Tanapongpipat, Weerawat Runguphan
Background: Interests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive alternative to the traditional production of transportation fuels. Here, we engineered Pichia pastoris, an industrial workhorse in heterologous enzyme production, to produce the biofuel isobutanol from two renewable carbon sources, glucose and glycerol...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29318200/genome-and-metabolic-engineering-in-non-conventional-yeasts-current-advances-and-applications
#12
REVIEW
Ann-Kathrin Löbs, Cory Schwartz, Ian Wheeldon
Microbial production of chemicals and proteins from biomass-derived and waste sugar streams is a rapidly growing area of research and development. While the model yeast Saccharomyces cerevisiae is an excellent host for the conversion of glucose to ethanol, production of other chemicals from alternative substrates often requires extensive strain engineering. To avoid complex and intensive engineering of S. cerevisiae, other yeasts are often selected as hosts for bioprocessing based on their natural capacity to produce a desired product: for example, the efficient production and secretion of proteins, lipids, and primary metabolites that have value as commodity chemicals...
September 2017: Synthetic and Systems Biotechnology
https://www.readbyqxmd.com/read/29318199/engineering-bacteria-for-enhanced-polyhydroxyalkanoates-pha-biosynthesis
#13
REVIEW
Guo-Qiang Chen, Xiao-Ran Jiang
Polyhydroxyalkanoates (PHA) have been produced by some bacteria as bioplastics for many years. Yet their commercialization is still on the way. A few issues are related to the difficulty of PHA commercialization: namely, high cost and instabilities on molecular weights (Mw) and structures, thus instability on thermo-mechanical properties. The high cost is the result of complicated bioprocessing associated with sterilization, low conversion of carbon substrates to PHA products, and slow growth of microorganisms as well as difficulty of downstream separation...
September 2017: Synthetic and Systems Biotechnology
https://www.readbyqxmd.com/read/29316500/temperature-dependent-cellulase-adsorption-on-lignin-from-sugarcane-bagasse
#14
Ariane Zanchetta, Antonio Carlos Freitas Dos Santos, Eduardo Ximenes, Christiane da Costa Carreira Nunes, Maurício Boscolo, Eleni Gomes, Michael R Ladisch
Extents of adsorption of cellulolytic enzymes on lignin, derived from sugarcane bagasse, were an inverse function of incubation temperature and varied with type of lignin extraction. At 45 °C, lignin derived from acid hydrolyzed liquid hot water pretreated bagasse completely adsorbed cellulolytic enzymes from Trichoderma reesei within 90 min. Lignin derived from enzyme hydrolyzed liquid hot water pretreated bagasse adsorbed only 60% of T. reesei endoglucanase, exoglucanase and β-glucosidase activities...
December 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29315476/consolidated-bioprocessing-of-lignocellulosic-biomass-to-lactic-acid-by-a-synthetic-fungal-bacterial-consortium
#15
Robert Lawrence Shahab, Jeremy S Luterbacher, Simone Brethauer, Michael Hans-Peter Studer
Consolidated bioprocessing (CBP) of lignocellulosic feedstocks to platform chemicals requires complex metabolic processes, which are commonly executed by single genetically engineered microorganisms. Alternatively, synthetic consortia can be employed to compartmentalize the required metabolic functions among different specialized microorganisms as demonstrated in this work for the direct production of lactic acid from lignocellulosic biomass. We composed an artificial cross-kingdom consortium and co-cultivated the aerobic fungus Trichoderma reesei for the secretion of cellulolytic enzymes with facultative anaerobic lactic acid bacteria...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29313328/efficient-biosynthesis-of-natural-yellow-pigments-by-monascus-purpureus-in-a-novel-integrated-fermentation-system
#16
Jun Lv, Gao-Fei Qian, Lei Chen, Huan Liu, Hai-Xiao Xu, Gan-Rong Xu, Bo-Bo Zhang, Chan Zhang
Due to the increasing demand for healthy and safe food, Monascus spp. have gained much attention as a sustainable source of natural food colorant. In this study, a novel integrated fermentation system consisting of surfactant and in situ extractant was established for efficiently producing yellow pigments by M. purpureus sjs-6. The maximum production of Monascus yellow pigment (669.2 U/mL) was obtained when 40% soybean oil (as extractant) was supplied at the beginning and 5 g/L Span-80 (as surfactant) was supplied at the 72th h, resulting in production 27...
January 9, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29313024/evolving-trends-in-mab-production-processes
#17
REVIEW
Abhinav A Shukla, Leslie S Wolfe, Sigma S Mostafa, Carnley Norman
Monoclonal antibodies (mAbs) have established themselves as the leading biopharmaceutical therapeutic modality. The establishment of robust manufacturing platforms are key for antibody drug discovery efforts to seamlessly translate into clinical and commercial successes. Several drivers are influencing the design of mAb manufacturing processes. The advent of biosimilars is driving a desire to achieve lower cost of goods and globalize biologics manufacturing. High titers are now routinely achieved for mAbs in mammalian cell culture...
March 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/29311542/versatile-and-on-demand-biologics-co-production-in-yeast
#18
Jicong Cao, Pablo Perez-Pinera, Ky Lowenhaupt, Ming-Ru Wu, Oliver Purcell, Cesar de la Fuente-Nunez, Timothy K Lu
Current limitations to on-demand drug manufacturing can be addressed by technologies that streamline manufacturing processes. Combining the production of two or more drugs into a single batch could not only be useful for research, clinical studies, and urgent therapies but also effective when combination therapies are needed or where resources are scarce. Here we propose strategies to concurrently produce multiple biologics from yeast in single batches by multiplexing strain development, cell culture, separation, and purification...
January 8, 2018: Nature Communications
https://www.readbyqxmd.com/read/29311513/inhibition-of-mushroom-tyrosinase-activity-by-orsellinates
#19
Thiago Inácio Barros Lopes, Roberta Gomes Coelho, Neli Kika Honda
Several applications have been proposed for tyrosinase inhibitors in the pharmaceutical, food bioprocessing, and environmental industries. However, only a few compounds are known to serve as effective tyrosinase inhibitors. This study evaluated the tyrosinase-related activity of resorcinol (1), orcinol (2) lecanoric acid (3), and derivatives of this acid (4-15). Subjected to alcoholysis, lecanoric acid (3), a depside isolated from the lichen Parmotrema tinctorum, produces orsellinic acid (2,4-dihydroxy-6-methylbenzoic acid) (4) and orsellinates (2,4-dihydroxy-6-methyl benzoates) (5-15)...
2018: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29305513/production-of-4-hydroxybenzoic-acid-by-an-aerobic-growth-arrested-bioprocess-using-metabolically-engineered-corynebacterium-glutamicum
#20
Yukihiro Kitade, Ryoma Hashimoto, Masako Suda, Kazumi Hiraga, Masayuki Inui
Corynebacterium glutamicum was metabolically engineered to produce 4-hydroxybenzoic acid (4-HBA), a valuable aromatic compound used as a raw material for the production of liquid crystal polymers and paraben. C. glutamicum was found to have a higher tolerance to 4-HBA toxicity than previously reported hosts used for the production of genetically engineered 4-HBA. To obtain higher titers of 4-HBA, we employed a stepwise overexpression of all seven target genes in the shikimate pathway in C. glutamicum Specifically, multiple chromosomal integrations of a mutated aroG from Escherichia coli, encoding a DAHP synthase, and wild-type aroCKB from C...
January 5, 2018: Applied and Environmental Microbiology
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