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Burkholderia pseudomallei

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https://www.readbyqxmd.com/read/28225601/rational-optimization-of-diphenyl-ether-binding-kinetics-to-the-enoyl-acp-reductase-fabi1-from-burkholderia-pseudomallei
#1
Carla Maya Neckles, Sandra Eltschkner, Jason Ellsworth Cummings, Maria Hirschbeck, Fereidoon Daryaee, Gopal R Bommineni, Zhuo Zhang, Lauren A Spagnuolo, Weixuan Yu, Shabnam Davoodi, Richard A Slayden, Caroline Kisker, Peter J Tonge
There is growing awareness of the link between drug-target residence time and in vivo drug activity, and there are increasing efforts to determine the molecular factors that control the lifetime of a drug-target complex. Rational alterations in drug-target residence time require knowledge of both the ground and transition states on the inhibition reaction coordinate, and we have determined the structure-kinetic relationship for 22 ethyl or hexyl substituted diphenyl ethers that are slow-binding inhibitors of bpFabI1, the enoyl-ACP reductase FabI1 from Burkholderia pseudomallei...
February 22, 2017: Biochemistry
https://www.readbyqxmd.com/read/28216665/interleukin-10-inhibits-pro-inflammatory-cytokine-responses-and-killing-of-burkholderia-pseudomallei
#2
Bianca Kessler, Darawan Rinchai, Chidchamai Kewcharoenwong, Arnone Nithichanon, Rachael Biggart, Catherine M Hawrylowicz, Gregory J Bancroft, Ganjana Lertmemongkolchai
Melioidosis, caused by Burkholderia pseudomallei, is endemic in northeastern Thailand and Northern Australia. Severe septicemic melioidosis is associated with high levels of pro-inflammatory cytokines and is correlated with poor clinical outcomes. IL-10 is an immunoregulatory cytokine, which in other infections can control the expression of pro-inflammatory cytokines, but its role in melioidosis has not been addressed. Here, whole blood of healthy seropositive individuals (n = 75), living in N. E. Thailand was co-cultured with B...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28188208/quantitative-detection-of-burkholderia-pseudomallei-in-soil-samples-using-multiple-gene-targets-in-real-time-pcr
#3
A Göhler, T T Trung, V Hopf, C Kohler, J Hartleib, V Wuthiekanun, S J Peacock, D Limmathurotsakul, A Tuanyok, I Steinmetz
Burkholderia pseudomallei is present in the environment in many parts of the world and causes the often-fatal disease melioidosis. The sensitive detection and quantification of B. pseudomallei in the environment is a prerequisite to assess the risk of infection. We recently reported the direct detection of B. pseudomallei in soil samples using a qPCR targeting a single type three secretion system 1 (TTSS1) gene. Here, we extend the qPCR-based analysis of B. pseudomallei in soil by validating novel qPCR gene targets selected from comparative genomic analysis...
February 10, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28187198/two-stable-variants-of-burkholderia-pseudomallei-strain-mshr5848-express-broadly-divergent-in-vitro-phenotypes-associated-with-their-virulence-differences
#4
A A Shea, R C Bernhards, C K Cote, C J Chase, J W Koehler, C P Klimko, J T Ladner, D A Rozak, M J Wolcott, D P Fetterer, S J Kern, G I Koroleva, S P Lovett, G F Palacios, R G Toothman, J A Bozue, P L Worsham, S L Welkos
Burkholderia pseudomallei (Bp), the agent of melioidosis, causes disease ranging from acute and rapidly fatal to protracted and chronic. Bp is highly infectious by aerosol, can cause severe disease with nonspecific symptoms, and is naturally resistant to multiple antibiotics. However, no vaccine exists. Unlike many Bp strains, which exhibit random variability in traits such as colony morphology, Bp strain MSHR5848 exhibited two distinct and relatively stable colony morphologies on sheep blood agar plates: a smooth, glossy, pale yellow colony and a flat, rough, white colony...
2017: PloS One
https://www.readbyqxmd.com/read/28186697/complement-activated-vitronectin-enhances-the-invasion-of-non-phagocytic-cells-by-bacterial-pathogens-burkholderia-and-klebsiella
#5
Yi Han Tan, Akshamal Gamage, Yunn-Hwen Gan
Burkholderia pseudomallei is a serum-resistant Gram-negative bacterium capable of causing disseminated infections with metastatic complications. However, their interaction with non-phagocytic cells is poorly understood. We observed that exposure of B. pseudomallei and the closely related yet avirulent B. thailandensis to human plasma increased epithelial cell invasion by >20 fold. Enhanced invasion was primarily driven by a plasma factor which required a functional complement cascade, but surprisingly, was downstream of C3 mediated opsonisation...
February 10, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28179407/calprotectin-as-a-biomarker-for-melioidosis-disease-progression-and-management
#6
Mohan Natesan, Enoka Corea, Shivankari Krishnananthasivam, Harindra Darshana Sathkumara, Jennifer L Dankmeyer, Beverly K Dyas, Kei Amemiya, Aruna Dharshan De Silva, Robert G Ulrich
Melioidosis is a neglected tropical disease that is caused by the bacterium Burkholderia pseudomallei, and is underreported in many endemic countries. A long and costly administration of antibiotics is required to clear infections, and there is an unmet need for biomarkers to guide antibiotic treatment and increase the number of patients that complete therapy. We identified calprotectin as a lead biomarker of Burkholderia pseudomallei infections, and examined correlations between this serum protein and antibiotic treatment outcomes of melioidosis patients...
February 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28177319/expression-and-crystallographic-studies-of-d-glycero-%C3%AE-d-manno-heptose-1-phosphate-adenylyltransferase-from-burkholderia-pseudomallei
#7
Jimin Park, Hyojin Kim, Suwon Kim, Daeun Lee, Dong Hae Shin
The Gram-negative bacterium Burkholderia pseudomallei is the causative agent of melioidosis. D-glycero-β-D-manno-Heptose-1-phosphate adenylyltransferase (HldC) is the fourth enzyme of the ADP-L-glycero-β-D-manno-heptose biosynthesis pathway, which produces an essential carbohydrate comprising the inner core of lipopolysaccharide. Therefore, HldC is a potential target of antibiotics against melioidosis. In this study, HldC from B. pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data from a selenomethionine-substituted HldC crystal were also collected to 2...
February 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28166272/activation-of-mapk-erk-signaling-by-burkholderia-pseudomallei-cycle-inhibiting-factor-cif
#8
Mei Ying Ng, Mei Wang, Patrick J Casey, Yunn-Hwen Gan, Thilo Hagen
Cycle inhibiting factors (Cifs) are virulence proteins secreted by the type III secretion system of some Gram-negative pathogenic bacteria including Burkholderia pseudomallei. Cif is known to function to deamidate Nedd8, leading to inhibition of Cullin E3 ubiquitin ligases (CRL) and consequently induction of cell cycle arrest. Here we show that Cif can function as a potent activator of MAPK/ERK signaling without significant activation of other signaling pathways downstream of receptor tyrosine kinases. Importantly, we found that the ability of Cif to activate ERK is dependent on its deamidase activity, but independent of Cullin E3 ligase inhibition...
2017: PloS One
https://www.readbyqxmd.com/read/28166079/osteomyelitis-of-humerus-and-intramuscular-abscess-due-to-melioidosis
#9
G S Vijaykumar, P Thilakavathy, S S Jeremiah, G Vithiya
Melioidosis is a clinically diverse disease caused by gram negative bacterium Burkholderia pseudomallei. It is a potential bioterrorism agent. The high risk group includes the agricultural and construction workers whose contact with contaminated soil and water may expose them to bacteria. The clinical manifestations varies from asymptomatic infection to overwhelming sepsis. To diagnose melioidosis a high index of suspicion along with isolation and identification of the organism from the clinical samples is needed...
April 2016: Kathmandu University Medical Journal (KUMJ)
https://www.readbyqxmd.com/read/28141587/osteoarticular-and-soft-tissue-melioidosis-in-malaysia-clinical-characteristics-and-molecular-typing-of-the-causative-agent
#10
AbdelRahman Mohammad Zueter, Mahmoud Abumarzouq, Mohd Imran Yusof, Wan Faisham Wan Ismail, Azian Harun
INTRODUCTION: Melioidosis involving bone, joints, and soft tissue is rare and reported usually following dissemination of disease from infection elsewhere in the body; to a lesser degree, it can also be reported as the primary manifestation of melioidosis. METHODOLOGY: The orthopedic registry at Hospital University Sains Malaysia from 2008 until 2014 was retrospectively reviewed and was followed by molecular typing of Burkholderia pseudomallei. RESULTS: Out of 20 cases identified, 19 patients were confirmed to have osteoarticular and/or soft-tissue melioidosis...
January 30, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28138332/erratum-to-pediatric-suppurative-parotitis-caused-by-burkholderia-pseudomallei
#11
Zengzhen Fu, Yingzi Lin, Qiang Wu, Qianfeng Xia
[This corrects the article DOI: 10.1186/s40409-016-0086-3.].
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28124968/-accurate-diagnosis-of-pseudomonas-luteola-in-routine-microbiology-laboratory-on-the-occasion-of-two-isolates
#12
Muharrem Çiçek, Gülşen Hasçelik, H Kaan Müştak, K Serdar Diker, Burçin Şener
Pseudomonas luteola which was previously known as Chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. It is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. It has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. Thus, early and accurate identification of this rare species is important for the treatment and also to provide information about the epidemiology of P...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28115683/validation-of-a-monoclonal-antibody-based-immunofluorescent-assay-to-detect-burkholderia-pseudomallei-in-blood-cultures
#13
Adul Dulsuk, Suporn Paksanont, Adisak Sangchankoom, Peeraya Ekchariyawat, Rungnapa Phunpang, Yaowaruk Jutrakul, Narisara Chantratita, T Eoin West
BACKGROUND: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. METHODS: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016. RESULTS: Sensitivity of the IFA was 100% and specificity was 99...
January 22, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28112723/global-and-regional-dissemination-and-evolution-of-burkholderia-pseudomallei
#14
Claire Chewapreecha, Matthew T G Holden, Minna Vehkala, Niko Välimäki, Zhirong Yang, Simon R Harris, Alison E Mather, Apichai Tuanyok, Birgit De Smet, Simon Le Hello, Chantal Bizet, Mark Mayo, Vanaporn Wuthiekanun, Direk Limmathurotsakul, Rattanaphone Phetsouvanh, Brian G Spratt, Jukka Corander, Paul Keim, Gordon Dougan, David A B Dance, Bart J Currie, Julian Parkhill, Sharon J Peacock
The environmental bacterium Burkholderia pseudomallei causes an estimated 165,000 cases of human melioidosis per year worldwide and is also classified as a biothreat agent. We used whole genome sequences of 469 B. pseudomallei isolates from 30 countries collected over 79 years to explore its geographic transmission. Our data point to Australia as an early reservoir, with transmission to Southeast Asia followed by onward transmission to South Asia and East Asia. Repeated reintroductions were observed within the Malay Peninsula and between countries bordered by the Mekong River...
January 23, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28105344/molecular-tracking-investigation-of-melioidosis-cases-reveals-regional-endemicity-in-hainan-china
#15
Yingzi Lin, Qiang Wu, Xiang Liu, Sufang Dong, Lixian Wu, Hua Pei, Ke Xu, Qianfeng Xia
Sporadic cases of melioidosis have been reported in Hainan, China for decades; however, to the best of our knowledge, there are no accurate source-identification investigations confirming that melioidosis is endemic. Four indigenous melioidosis cases were identified, which prompted the performance of contact microbiologic and molecular techniques to evaluate endemicity. Environmental samples were collected from various locations surrounding each patient's residence. The samples were screened for Burkholderia pseudomallei (B...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28104185/causes-and-outcomes-of-sepsis-in-southeast-asia-a-multinational-multicentre-cross-sectional-study
#16
(no author information available yet)
BACKGROUND: Improved understanding of pathogens that cause sepsis would aid management and antimicrobial selection. In this study, we aimed to identify the causative pathogens of sepsis in southeast Asia. METHODS: In this multinational multicentre cross-sectional study of community-acquired sepsis and severe sepsis, we prospectively recruited children (age ≥30 days and <18 years) and adults (age ≥18 years) at 13 public hospitals in Indonesia (n=3), Thailand (n=4), and Vietnam (n=6)...
February 2017: Lancet Global Health
https://www.readbyqxmd.com/read/28096229/disseminated-melioidosis-in-the-head-and-neck
#17
Tze Ling Loh, Sergios Latis, Graeme Crossland, Hemi Patel
A 35-year-old man was admitted to an intensive care unit with unilateral facial swelling and septic shock after multiple presentations to the emergency department with non-specific unilateral pain over the parotid area. A CT scan of his neck showed diffuse right-sided facial soft tissue infection, mastoid effusion and temporal lobe cerebritis. The upper lobes of his lungs had cannonball lesions that were suggestive of septic lung metastases. Blood cultures and ear canal swabs were positive for Burkholderia pseudomallei The temporal lobe cerebritis eventually developed into an abscess, necessitating a cortical mastoidectomy, craniectomy and temporal lobectomy...
January 17, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28081127/transient-in-vivo-resistance-mechanisms-of-burkholderia-pseudomallei-to-ceftazidime-and-molecular-markers-for-monitoring-treatment-response
#18
Jason E Cummings, Richard A Slayden
Much is known about the mode of action of drugs and resistance mechanisms under laboratory growth conditions, but research on the bacterial transcriptional response to drug pressure in vivo or efficacious mode of action and transient resistance mechanisms of clinically employed drugs is limited. Accordingly, to assess active alternative metabolism and transient resistance mechanisms, and identify molecular markers of treatment response, the in vivo transcriptional response of Burkholderia pseudomallei 1026b to treatment with ceftazidime in infected lungs was compared to the in vitro bacterial response in the presence of drug...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28067495/upconversion-nanocrystals-mediated-lateral-flow-nanoplatform-for-in-vitro-detection
#19
Zhiqin Liang, Xiaochen Wang, Wei Zhu, Pingping Zhang, Yongxin Yang, Chongyun Sun, Junjie Zhang, Xinrui Wang, Zheng Xu, Yong Zhao, Ruifu Yang, Suling Zhao, Lei Zhou
Upconversion phosphors (UCPs) that are free from interference from biological sample autofluorescence have attracted attention for in vivo and in vitro bioapplications. However, UCPs need to be water-dispersible, nanosized, and highly luminous to realize broad applications. Therefore, the aim of this research is to develop UCPs that meet these comprehensive criteria for in vitro diagnosis. To combine nano size with high luminous intensity, β-NaYF4:Yb(3+),Er(3+) upconversion nanocrystals (UCNPs) codoped with Li(+) and K(+) are prepared that display high upconversion intensities as well as small size...
February 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28066900/boosting-of-post-exposure-human-t-cell-and-b-cell-recall-responses-in-vivo-by-burkholderia-pseudomallei-related-proteins
#20
Arnone Nithichanon, Louise J Gourlay, Gregory J Bancroft, Manabu Ato, Yoshimasa Takahashi, Ganjana Lertmemongkolchai
Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease with high incidence and mortality in South East Asia and northern Australia. To date there is no protective vaccine and antibiotic treatment is prolonged and not always effective. Most people living in endemic areas have been exposed to the bacteria and have developed some immunity, which may have helped to prevent disease. Here, we used a humanized mouse model (hu-PBL-SCID), reconstituted with human peripheral blood mononuclear cells from seropositive donors, to illustrate the potential of three known antigens (FliC, OmpA and N-PilO2) for boosting both T-cell and B-cell immune responses...
January 9, 2017: Immunology
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