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https://www.readbyqxmd.com/read/28620820/cytoglobin-inhibits-migration-through-pi3k-akt-mtor-pathway-in-fibroblast-cells
#1
Selami Demirci, Ayşegül Doğan, Hüseyin Apdik, Emre Can Tuysuz, Sukru Gulluoglu, Omer Faruk Bayrak, Fikrettin Şahin
Cell proliferation and migration are crucial in many physiological processes including development, cancer, tissue repair, and wound healing. Cell migration is regulated by several signaling molecules. Identification of genes related to cell migration is required to understand molecular mechanism of non-healing chronic wounds which is a major concern in clinics. In the current study, the role of cytoglobin (CYGB) gene in fıbroblast cell migration and proliferation was described. L929 mouse fibroblast cells were transduced with lentiviral particles for CYGB and GFP, and analyzed for cell proliferation and migration ability...
June 15, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28593139/oxygen-binding-and-nitric-oxide-dioxygenase-activity-of-cytoglobin-are-altered-to-different-extents-by-cysteine-modification
#2
Danlei Zhou, Craig Hemann, James Boslett, Aiqin Luo, Jay L Zweier, Xiaoping Liu
Cytoglobin (Cygb), like other members of the globin family, is a nitric oxide (NO) dioxygenase, metabolizing NO in an oxygen (O2)-dependent manner. We examined the effect of modification of cysteine sulfhydryl groups of Cygb on its O2 binding and NO dioxygenase activity. The two cysteine sulfhydryls of Cygb were modified to form either an intramolecular disulfide bond (Cygb_SS), thioether bonds to N-ethylmaleimide (NEM; Cygb_SC), or were maintained as free SH groups (Cygb_SH). It was observed that the NO dioxygenase activity of Cygb only slightly changed (~ 25%) while the P50 of O2 binding to Cygb changed over four-fold with these modifications...
June 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28540429/identification-of-markers-for-quiescent-pancreatic-stellate-cells-in-the-normal-human-pancreas
#3
Michael Friberg Bruun Nielsen, Michael Bau Mortensen, Sönke Detlefsen
Pancreatic stellate cells (PSCs) play a central role as source of fibrogenic cells in pancreatic cancer and chronic pancreatitis. In contrast to quiescent hepatic stellate cells (qHSCs), a specific marker for quiescent PSCs (qPSCs) that can be used in formalin-fixed and paraffin embedded (FFPE) normal human pancreatic tissue has not been identified. The aim of this study was to identify a marker enabling the identification of qPSCs in normal human FFPE pancreatic tissue. Immunohistochemical (IHC), double-IHC, immunofluorescence (IF) and double-IF analyses were carried out using a tissue microarray consisting of cores with normal human pancreatic tissue...
May 25, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28501743/antarctic-fish-versus-human-cytoglobins-the-same-but-yet-so-different
#4
Bert Cuypers, Stijn Vermeylen, Dietmar Hammerschmid, Stanislav Trashin, Vanoushe Rahemi, Albert Konijnenberg, Amy De Schutter, C-H Christina Cheng, Daniela Giordano, Cinzia Verde, Karolien De Wael, Frank Sobott, Sylvia Dewilde, Sabine Van Doorslaer
The cytoglobins of the Antarctic fish Chaenocephalus aceratus and Dissostichus mawsoni have many features in common with human cytoglobin. These cytoglobins are heme proteins in which the ferric and ferrous forms have a characteristic hexacoordination of the heme iron, i.e. axial ligation of two endogenous histidine residues, as confirmed by electron paramagnetic resonance, resonance Raman and optical absorption spectroscopy. The combined spectroscopic analysis revealed only small variations in the heme-pocket structure, in line with the small variations observed for the redox potential...
April 28, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28428250/functional-roles-of-globin-proteins-in-hypoxia-tolerant-ectothermic-vertebrates
#5
Angela Fago
Globins are heme-containing proteins ubiquitously expressed in vertebrates, where they serve a broad range of biological functions, directly or indirectly related to the tight control of oxygen levels and its toxic products in vivo. Perhaps the most investigated of all proteins, hemoglobin and myoglobin are primarily involved in oxygen transport and storage, but also in facilitating arterial vasodilation, suppressing mitochondrial respiration and preventing tissue oxidative damage via accessory redox enzymatic activities during hypoxia...
April 20, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28393874/cytoglobin-regulates-blood-pressure-and-vascular-tone-through-nitric-oxide-metabolism-in-the-vascular-wall
#6
Xiaoping Liu, Mohamed A El-Mahdy, James Boslett, Saradhadevi Varadharaj, Craig Hemann, Tamer M Abdelghany, Raed S Ismail, Sean C Little, Danlei Zhou, Le Thi Thanh Thuy, Norifumi Kawada, Jay L Zweier
The identity of the specific nitric oxide dioxygenase (NOD) that serves as the main in vivo regulator of O2-dependent NO degradation in smooth muscle remains elusive. Cytoglobin (Cygb) is a recently discovered globin expressed in fibroblasts and smooth muscle cells with unknown function. Cygb, coupled with a cellular reducing system, efficiently regulates the rate of NO consumption by metabolizing NO in an O2-dependent manner with decreased NO consumption in physiological hypoxia. Here we show that Cygb is a major regulator of NO degradation and cardiovascular tone...
April 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28258342/col1a1-prpf40a-and-ucp2-correlate-with-hypoxia-markers-in-non-small-cell-lung-cancer
#7
Urszula Oleksiewicz, Triantafillos Liloglou, Kalliopi-Maria Tasopoulou, Nikoleta Daskoulidou, John R Gosney, John K Field, George Xinarianos
PURPOSE: Collagen 1A1 (COL1A1), RNA-binding and pre-mRNA Processing Factor (PRPF40A), and Uncoupling Protein 2 (UCP2) were identified as downstream effectors of cytoglobin (CYGB), which was shown implicated in tumour biology. Although these three genes have been previously associated with cancer, little is known about their status in lung malignancies. METHODS: Hereby, we investigated the expression and promoter methylation of COL1A1, PRPF40A, and UCP2 in 156 non-small cell lung cancer (NSCLC) and adjacent normal tissues...
March 3, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28218331/ultrafast-photochemistry-of-the-bc1-complex
#8
Marten H Vos, Brandon J Reeder, Fevzi Daldal, Ursula Liebl
We present a full investigation of ultrafast light-induced events in the membraneous cytochrome bc1 complex by transient absorption spectroscopy. This energy-transducing complex harbors four redox-active components per monomer: heme c1, two 6-coordinate b-hemes and a [2Fe-2S] cluster. Using excitation of these components in different ratios under various excitation conditions, probing in the full visible range and under three well-defined redox conditions, we demonstrate that for all ferrous hemes of the complex photodissociation of axial ligands takes place and that they rebind in 5-7 ps, as in other 6-coordinate heme proteins, including cytoglobin, which is included as a reference in this study...
March 1, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28157235/possible-involvement-of-nitric-oxide-in-enhanced-liver-injury-and-fibrogenesis-during-cholestasis-in-cytoglobin-deficient-mice
#9
Tuong Thi Van Thuy, Le Thi Thanh Thuy, Katsutoshi Yoshizato, Norifumi Kawada
This study clarified the role of Cygb, the fourth globin in mammals originally discovered in rat hepatic stellate cells (HSCs), in cholestatic liver disease. Bile duct ligation (BDL) augmented inflammatory reactions as revealed by increased infiltrating neutrophils, CD68(+)-macrophages, and chemokine expression in Cygb(-/-) mice. In these mice, impairment of bile canalicular indicated by the loss of CD10 expression, down-regulation of bile salt transporters, increased total bile acid, and massive apoptotic and necrotic hepatocytes occurred with the release of cytochrome c, activation of caspase 3, resulting in reduced animal survival compared to wild-type mice...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28128325/protective-effects-of-recombinant-human-cytoglobin-against-chronic-alcohol-induced-liver-disease-in-vivo-and-in-vitro
#10
Jian Wen, Yongbin Wu, Wei Wei, Zhen Li, Ping Wang, Shiwei Zhu, Wenqi Dong
Alcoholic liver disease (ALD) is an important worldwide public health issue with no satisfying treatment available since now. Here we explore the effects of recombinant human cytoglobin (rhCygb) on chronic alcohol-induced liver injury and the underlying mechanisms. In vivo studies showed that rhCygb was able to ameliorate alcohol-induced liver injury, significantly reversed increased serum index (ALT, AST, TG, TC and LDL-C) and decreased serum HDL-C. Histopathology observation of the liver of rats treated with rhCygb confirmed the biochemical data...
January 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28099346/liuwei-dihuang-pill-treats-diabetic-nephropathy-in-rats-by-inhibiting-of-tgf-%C3%AE-smads-mapk-and-nf-kb-and-upregulating-expression-of-cytoglobin-in-renal-tissues
#11
Zhong Ju Xu, Shi Shu, Zhi Jie Li, Yu Min Liu, Rui Yi Zhang, Yue Zhang
Liuwei Dihuang pill (LDP) was assessed for its effects on renal deficiency.90 STZ induced DN rats were divided into groups (n = 22) without treatment (STZ) and LDP treated (STZ-L) (n = 23), Zhenwu decoction treated (STZ-Z) (n = 22), and valsartan treated (STZ-V) (n = 23) groups, with 16 normal control rats. Total urine protein (TP), blood urea nitrogen (BUN), and serum creatinine (Cr) were measured. Superoxide dismutase (SOD), nitric oxide synthase (NOS), and malondialdehyde (MDA) concentrations as well as expression/phosphorylation of SMAD3, SMAD2, and α-SMA, TGF-β, RI /II, P38, ERK, and NF-kB in renal tissues were determined...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27928650/cytoglobin-a-potential-marker-for-adipogenic-differentiation-in-preadipocytes-in-vitro
#12
Ayşegül Doğan, Selami Demirci, Binnur Kıratlı, Fikrettin Şahin
Obesity, mainly characterized by the excess fat storage, is a global health problem resulting in serious morbidity and mortality. Identification of molecular mechanisms in adipogenic differentiation pathway might lead to development of new strategies for diagnosis, prevention and therapy of obesity and associated diseases. Discovery of new genes and proteins in the differentiation pathway could help to understand the key specific regulators of the adipogenesis. Cytoglobin (Cygb), identified as a new globin family member protein, is expressed in various tissues...
February 2017: Cytotechnology
https://www.readbyqxmd.com/read/27867483/brain-globins-in-physiology-and-pathology
#13
REVIEW
Luo-Kun Xie, Shao-Hua Yang
Globins are globular proteins for either transport or storage of oxygen which are critical for cellular metabolism. Four globins have been identified in rodent and human brains. Among them, neuroglobin, cytoglobin and hemoglobin chains are constitutively expressed in normal brain, while myoglobin is only expressed in some neurological disorders. Studies on the molecular structure, expression and functional features of these brain globins indicated that they may play crucial roles in maintenance of neural cell survival and activity, including neurons and astrocytes...
July 2016: Medical Gas Research
https://www.readbyqxmd.com/read/27678372/the-novel-thiosemicarbazone-di-2-pyridylketone-4-cyclohexyl-4-methyl-3-thiosemicarbazone-dpc-inhibits-neuroblastoma-growth-in-vitro-and-in-vivo-via-multiple-mechanisms
#14
Zhu-Ling Guo, Des R Richardson, Danuta S Kalinowski, Zaklina Kovacevic, Kian Cheng Tan-Un, Godfrey Chi-Fung Chan
BACKGROUND: Neuroblastoma is a relatively common and highly belligerent childhood tumor with poor prognosis by current therapeutic approaches. A novel anti-cancer agent of the di-2-pyridylketone thiosemicarbazone series, namely di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT), demonstrates promising anti-tumor activity. Recently, a second-generation analogue, namely di-2-pyridylketone 4-cyclohexyl-4-methyl-3-thiosemicarbazone (DpC), has entered multi-center clinical trials for the treatment of advanced and resistant tumors...
September 27, 2016: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/27637274/redox-and-peroxidase-activities-of-the-hemoglobin-superfamily-relevance-to-health-and-disease
#15
Brandon J Reeder
SIGNIFICANCE: Erythrocyte hemoglobin (Hb) and myocyte myoglobin, although primarily oxygen-carrying proteins, have the capacity to do redox chemistry. Such redox activity in the wider family of globins now appears to have important associations with the mechanisms of cell stress response. In turn, an understanding of such mechanisms in vivo may have a potential in the understanding of cancer therapy resistance and neurodegenerative disorders such as Alzheimer's. Recent Advances: There has been an enhanced understanding of the redox chemistry of the globin superfamily in recent years, leading to advances in development of Hb-based blood substitutes and in hypotheses relating to specific disease mechanisms...
May 10, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/27512111/unusual-diversity-of-myoglobin-genes-in-the-lungfish
#16
Jonas Koch, Julia Lüdemann, Rieke Spies, Marco Last, Chris T Amemiya, Thorsten Burmester
Myoglobin is a respiratory protein that serves as a model system in a variety of biological fields. Its main function is to deliver and store O2 in the heart and skeletal muscles, but myoglobin is also instrumental in homeostasis of nitric oxide (NO) and detoxification of reactive oxygen species (ROS). Almost every vertebrate harbors a single myoglobin gene; only some cyprinid fishes have two recently duplicated myoglobin genes. Here we show that the West African lungfish Protopterus annectens has at least seven distinct myoglobin genes (PanMb1-7), which diverged early in the evolution of lungfish and showed an enhanced evolutionary rate...
December 2016: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/27496969/dna-hypermethylation-analysis-in-sputum-of-asymptomatic-subjects-at-risk-for-lung-cancer-participating-in-the-nelson-trial-argument-for-maximum-screening-interval-of-2%C3%A2-years
#17
RANDOMIZED CONTROLLED TRIAL
A Jasmijn Hubers, Daniëlle A M Heideman, Sylvia Duin, Birgit I Witte, Harry J de Koning, Harry J M Groen, Clemens F M Prinsen, Anne S Bolijn, Mandy Wouters, Susanne E van der Meer, Renske D M Steenbergen, Peter J F Snijders, Anne Uyterlinde, Hans Berkhof, Egbert F Smit, Erik Thunnissen
AIMS: Lung cancer is the major contributor to cancer mortality due to metastasised disease at time of presentation. The current study investigated DNA hypermethylation of biomarkers RASSF1A, APC, cytoglobin, 3OST2, FAM19A4, PHACTR3 and PRDM14 in sputum of asymptomatic high-risk individuals from the NELSON lung cancer low-dose spiral CT screening trial to detect lung cancer at preclinical stage. METHODS: Subjects were selected with (i) lung cancer in follow-up (cases; n=65), (ii) minor cytological aberrations (controls; n=120) and (iii) a random selection of subjects without cytological aberrations (controls; n=99)...
March 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27441981/evaluation-of-globins-expression-in-brain-heart-and-lung-in-rats-exposed-to-side-stream-cigarette-smoke
#18
Barbara Tae, Kelen Carneiro Oliveira, Rodrigo Rodrigues da Conceição, Vitor Engrácia Valenti, Janaina Sena de Souza, Roberto Laureano-Melo, Monica Akemi Sato, Rui Monteiro de Barros Maciel, Gisele Giannocco
The side stream cigarette smoke (SSCS) is a contributing factor in the pathogenesis of cigarette smoking-induced toxicity. Hemoglobin (Hb), myoglobin (Mb), neuroglobin (Ngb), and cytoglobin (Cygb) are globins with different distributions and functions in the tissues and have similar actions by providing O2 (oxygen) for respiratory chain, detoxification of ROS and nitric oxide (NO), and protect tissues against irreversible lesions. We aimed to investigate the effects of SSCS exposure on gene and protein expression of Ngb, Cygb, and Mb in different tissue...
April 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/27431913/changes-in-mitochondrial-stability-during-the-progression-of-the-barrett-s-esophagus-disease-sequence
#19
N J O'Farrell, R Feighery, S L Picardo, N Lynam-Lennon, M Biniecka, S A McGarrigle, J J Phelan, F MacCarthy, D O'Toole, E J Fox, N Ravi, J V Reynolds, J O'Sullivan
BACKGROUND: Barrett's esophagus follows the classic step-wise progression of metaplasia-dysplasia-adenocarcinoma. While Barrett's esophagus is a leading known risk factor for esophageal adenocarcinoma, the pathogenesis of this disease sequence is poorly understood. Mitochondria are highly susceptible to mutations due to high levels of reactive oxygen species (ROS) coupled with low levels of DNA repair. The timing and levels of mitochondria instability and dysfunction across the Barrett's disease progression is under studied...
2016: BMC Cancer
https://www.readbyqxmd.com/read/27407144/globin-x-is-a-six-coordinate-globin-that-reduces-nitrite-to-nitric-oxide-in-fish-red-blood-cells
#20
Paola Corti, Jianmin Xue, Jesús Tejero, Nadeem Wajih, Ming Sun, Donna B Stolz, Michael Tsang, Daniel B Kim-Shapiro, Mark T Gladwin
The discovery of novel globins in diverse organisms has stimulated intense interest in their evolved function, beyond oxygen binding. Globin X (GbX) is a protein found in fish, amphibians, and reptiles that diverged from a common ancestor of mammalian hemoglobins and myoglobins. Like mammalian neuroglobin, GbX was first designated as a neuronal globin in fish and exhibits six-coordinate heme geometry, suggesting a role in intracellular electron transfer reactions rather than oxygen binding. Here, we report that GbX to our knowledge is the first six-coordinate globin and the first globin protein apart from hemoglobin, found in vertebrate RBCs...
July 26, 2016: Proceedings of the National Academy of Sciences of the United States of America
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