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https://www.readbyqxmd.com/read/28954806/cytoglobin-at-the-crossroads-of-vascular-remodeling
#1
EDITORIAL
Matthew B Amdahl, Anthony W DeMartino, Jesús Tejero, Mark T Gladwin
No abstract text is available yet for this article.
October 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28954528/recombinant-human-cytoglobin-prevents-atherosclerosis-by-regulating-lipid-metabolism-and-oxidative-stress
#2
Lingling Ou, Xin Li, Baihong Chen, Zhenhuang Ge, Junyi Zhang, Ye Zhang, Gaotai Cai, Zhen Li, Ping Wang, Wenqi Dong
Atherosclerosis is a chronic inflammatory vascular disease characterized by lipid accumulation and endothelial dysfunction. Cytoglobin has been shown to exert protective effects under oxidative stress conditions. The aim of this study was to determine whether recombinant human cytoglobin (rhCYGB) has protective effects against atherosclerosis. We intraperitoneally injected rhCYGB (0, 4, or 7 mg/kg BW) into the atherosclerotic rats daily for 60 days. The rhCYGB injections reduced low-density lipoprotein cholesterol (LDL-C) levels and increased high-density lipoprotein cholesterol levels in a dose-dependent manner, rhCYGB (7 mg/kg) significantly attenuated atherosclerosis...
January 1, 2017: Journal of Cardiovascular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28929379/erratum-to-anatomical-specificity-of-the-brain-in-the-modulation-of-neuroglobin-and-cytoglobin-genes-after-chronic-bisphenol-a-exposure
#3
Rodrigo Rodrigues da Conceição, Janaina Sena de Souza, Kelen Carneiro de Oliveira, Rui Monteiro de Barros Maciel, Marco Aurélio Romano, Renata Marino Romano, Magnus Régios Dias da Silva, Maria Izabel Chiamolera, Gisele Giannocco
No abstract text is available yet for this article.
September 20, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28918749/effect-of-low-temperature-on-globin-expression-respiratory-metabolic-enzyme-activities-and-gill-structure-of-litopenaeus-vannamei
#4
Meng Wu, Nan Chen, Chun-Xiao Huang, Yan He, Yong-Zhen Zhao, Xiao-Han Chen, Xiu-Li Chen, Huan-Ling Wang
Low temperature frequently influences growth, development, and even survival of aquatic animals. In the present study, physiological and molecular responses to low temperature in Litopenaeus vannamei were investigated. The cDNA sequences of two oxygen-carrying proteins, cytoglobin (Cygb) and neuroglobin (Ngb), were isolated. Protein structure analysis revealed that both proteins share a globin superfamily domain. Real-time PCR analysis indicated that Cygb and Ngb mRNA levels gradually increased during decrease in temperatures from 25 to 15°C and then decreased at 10°C in muscle, brain, stomach, and heart, except for a continuing increase in gills, whereas they showed a different expression trend in the hepatopancreas...
July 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28916723/fibroblast-growth-factor-2-fgf2-regulates-cytoglobin-expression-and-activation-of-human-hepatic-stellate-cells-via-jnk-signaling
#5
Misako Sato-Matsubara, Tsutomu Matsubara, Atsuko Daikoku, Yoshinori Okina, Lisa Longato, Krista Rombouts, Le Thi Thanh Thuy, Jun Adachi, Takeshi Tomonaga, Kazuo Ikeda, Katsutoshi Yoshizato, Massimo Pinzani, Norifumi Kawada
Cytoglobin (CYGB) belongs to the mammalian globin family and is exclusively expressed in hepatic stellate cells (HSCs) in the liver. In addition to its gas-binding ability, CYGB is relevant to hepatic inflammation, fibrosis, and cancer because of its antioxidative properties; however, the regulation of CYGB gene expression remains unknown. Here, we sought to identify factors that induce CYGB expression in HSCs and to clarify the molecular mechanism involved. We used the human HSC cell line HHSteC and primary human HSCs isolated from intact human liver tissues...
September 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28883470/cytoglobin-promotes-cardiac-progenitor-cell-survival-against-oxidative-stress-via-the-upregulation-of-the-nf%C3%AE%C2%BAb-inos-signal-pathway-and-nitric-oxide-production
#6
Shuning Zhang, Xiuchun Li, Frances L Jourd'heuil, Shunlin Qu, Neil Devejian, Edward Bennett, David Jourd'heuil, Chuanxi Cai
Human cardiac stem/progenitor cells (hCPCs) may serve in regenerative medicine to repair the infarcted heart. However, this approach is severely limited by the poor survival of donor cells. Recent studies suggest that the mammalian globin cytoglobin (CYGB) regulates nitric oxide (NO) metabolism and cell death. In the present study, we found that CYGB is expressed in hCPCs. Through molecular approaches aimed at increasing or decreasing CYGB expression in hCPCs, we found that CYGB functions as a pro-survival factor in response to oxidative stress...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28798140/the-hemoglobin-homolog-cytoglobin-in-smooth-muscle-inhibits-apoptosis-and-regulates-vascular-remodeling
#7
Frances L Jourd'heuil, Haiyan Xu, Timothy Reilly, Keneta McKellar, Chaymae El Alaoui, Julia Steppich, Yong Feng Liu, Wen Zhao, Roman Ginnan, David Conti, Reynold Lopez-Soler, Arif Asif, Rebecca K Keller, John J Schwarz, Le Thi Thanh Thuy, Norifumi Kawada, Xiaochun Long, Harold A Singer, David Jourd'heuil
OBJECTIVE: The role of hemoglobin and myoglobin in the cardiovascular system is well established, yet other globins in this context are poorly characterized. Here, we examined the expression and function of cytoglobin (CYGB) during vascular injury. APPROACH AND RESULTS: We characterized CYGB content in intact vessels and primary vascular smooth muscle (VSM) cells and used 2 different vascular injury models to examine the functional significance of CYGB in vivo. We found that CYGB was strongly expressed in medial arterial VSM and human veins...
October 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28721559/anatomical-specificity-of-the-brain-in-the-modulation-of-neuroglobin-and-cytoglobin-genes-after-chronic-bisphenol-a-exposure
#8
Rodrigo Rodrigues da Conceição, Janaina Sena de Souza, Kelen Carneiro de Oliveira, Rui Monteiro de Barros Maciel, Marco Aurélio Romano, Renata Marino Romano, Magnus Régios Dias da Silva, Maria Izabel Chiamolera, Gisele Giannocco
The aim of this study was to investigate the influence of Bisphenol A (BPA) exposure on Neuroglobin (Ngb) and Cytoglobin (Cygb) as well as oxidative stress gene expression in the cerebellum, hippocampus, hypothalamus and cortex. Male Wistar rats were randomly divided into 3 groups: Control and two groups receiving 2 different daily BPA dosages, 5 or 25 mg/kg from postnatal day 50 (PND50) through PND90 and they were euthanized at PND105. In the cortex, we found an increase in Ngb gene expression and also in superoxide dismutase 1 and Catalase (Cat)...
July 18, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28671819/efficient-reduction-of-vertebrate-cytoglobins-by-the-cytochrome-b5-cytochrome-b5-reductase-nadh-system
#9
COMPARATIVE STUDY
Matthew B Amdahl, Courtney E Sparacino-Watkins, Paola Corti, Mark T Gladwin, Jesús Tejero
Cytoglobin is a heme-containing protein ubiquitous in mammalian tissues. Unlike the evolutionarily related proteins hemoglobin and myoglobin, cytoglobin shows a six-coordinated heme binding, with the heme iron coordinated by two histidine side chains. Cytoglobin is involved in cytoprotection pathways through yet undefined mechanisms, and it has recently been demonstrated that cytoglobin has redox signaling properties via nitric oxide (NO) and nitrite metabolism. The reduced, ferrous cytoglobin can bind oxygen and will react with NO in a dioxygenation reaction to form nitrate, which dampens NO signaling...
August 1, 2017: Biochemistry
https://www.readbyqxmd.com/read/28662027/the-different-expressed-serum-proteins-in-rhcygb-treated-rat-model-of-liver-fibrosis-by-the-optimized-two-dimensional-gel-electrophoresis
#10
Gaotai Cai, Bohong Chen, Zhen Li, Wei Wei, Ping Wang, Wenqi Dong
Liver fibrosis, a common pathological process of chronic liver diseases, is the final stage of liver dysfunction that has severely deleterious impact on human health. Cytoglobin was first discovered in 2001 by proteomic analysis in rat stellate cells and was reported to play an important role in controlling tissue fibrosis. However, the mechanism by which cytoglobin inhibits or reverses the progression of fibrosis remains unclear. The present study examines the effect of recombinant human cytoblobin (rhCygb) in a rat model of liver fibrosis...
2017: PloS One
https://www.readbyqxmd.com/read/28620820/cytoglobin-inhibits-migration-through-pi3k-akt-mtor-pathway-in-fibroblast-cells
#11
Selami Demirci, Ayşegül Doğan, Hüseyin Apdik, Emre Can Tuysuz, Sukru Gulluoglu, Omer Faruk Bayrak, Fikrettin Şahin
Cell proliferation and migration are crucial in many physiological processes including development, cancer, tissue repair, and wound healing. Cell migration is regulated by several signaling molecules. Identification of genes related to cell migration is required to understand molecular mechanism of non-healing chronic wounds which is a major concern in clinics. In the current study, the role of cytoglobin (CYGB) gene in fıbroblast cell migration and proliferation was described. L929 mouse fibroblast cells were transduced with lentiviral particles for CYGB and GFP, and analyzed for cell proliferation and migration ability...
June 15, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28593139/oxygen-binding-and-nitric-oxide-dioxygenase-activity-of-cytoglobin-are-altered-to-different-extents-by-cysteine-modification
#12
Danlei Zhou, Craig Hemann, James Boslett, Aiqin Luo, Jay L Zweier, Xiaoping Liu
Cytoglobin (Cygb), like other members of the globin family, is a nitric oxide (NO) dioxygenase, metabolizing NO in an oxygen (O2)-dependent manner. We examined the effect of modification of cysteine sulfhydryl groups of Cygb on its O2 binding and NO dioxygenase activity. The two cysteine sulfhydryls of Cygb were modified to form either an intramolecular disulfide bond (Cygb_SS), thioether bonds to N-ethylmaleimide (NEM; Cygb_SC), or were maintained as free SH groups (Cygb_SH). It was observed that the NO dioxygenase activity of Cygb only slightly changed (~ 25%) while the P50 of O2 binding to Cygb changed over four-fold with these modifications...
June 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28540429/identification-of-markers-for-quiescent-pancreatic-stellate-cells-in-the-normal-human-pancreas
#13
Michael Friberg Bruun Nielsen, Michael Bau Mortensen, Sönke Detlefsen
Pancreatic stellate cells (PSCs) play a central role as source of fibrogenic cells in pancreatic cancer and chronic pancreatitis. In contrast to quiescent hepatic stellate cells (qHSCs), a specific marker for quiescent PSCs (qPSCs) that can be used in formalin-fixed and paraffin embedded (FFPE) normal human pancreatic tissue has not been identified. The aim of this study was to identify a marker enabling the identification of qPSCs in normal human FFPE pancreatic tissue. Immunohistochemical (IHC), double-IHC, immunofluorescence (IF) and double-IF analyses were carried out using a tissue microarray consisting of cores with normal human pancreatic tissue...
May 25, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28501743/antarctic-fish-versus-human-cytoglobins-the-same-but-yet-so-different
#14
Bert Cuypers, Stijn Vermeylen, Dietmar Hammerschmid, Stanislav Trashin, Vanoushe Rahemi, Albert Konijnenberg, Amy De Schutter, C-H Christina Cheng, Daniela Giordano, Cinzia Verde, Karolien De Wael, Frank Sobott, Sylvia Dewilde, Sabine Van Doorslaer
The cytoglobins of the Antarctic fish Chaenocephalus aceratus and Dissostichus mawsoni have many features in common with human cytoglobin. These cytoglobins are heme proteins in which the ferric and ferrous forms have a characteristic hexacoordination of the heme iron, i.e. axial ligation of two endogenous histidine residues, as confirmed by electron paramagnetic resonance, resonance Raman and optical absorption spectroscopy. The combined spectroscopic analysis revealed only small variations in the heme-pocket structure, in line with the small variations observed for the redox potential...
April 28, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28428250/functional-roles-of-globin-proteins-in-hypoxia-tolerant-ectothermic-vertebrates
#15
Angela Fago
Globins are heme-containing proteins ubiquitously expressed in vertebrates, where they serve a broad range of biological functions, directly or indirectly related to the tight control of oxygen levels and its toxic products in vivo. Perhaps the most investigated of all proteins, hemoglobin and myoglobin are primarily involved in oxygen transport and storage, but also in facilitating arterial vasodilation, suppressing mitochondrial respiration and preventing tissue oxidative damage via accessory redox enzymatic activities during hypoxia...
April 20, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28393874/cytoglobin-regulates-blood-pressure-and-vascular-tone-through-nitric-oxide-metabolism-in-the-vascular-wall
#16
Xiaoping Liu, Mohamed A El-Mahdy, James Boslett, Saradhadevi Varadharaj, Craig Hemann, Tamer M Abdelghany, Raed S Ismail, Sean C Little, Danlei Zhou, Le Thi Thanh Thuy, Norifumi Kawada, Jay L Zweier
The identity of the specific nitric oxide dioxygenase (NOD) that serves as the main in vivo regulator of O2-dependent NO degradation in smooth muscle remains elusive. Cytoglobin (Cygb) is a recently discovered globin expressed in fibroblasts and smooth muscle cells with unknown function. Cygb, coupled with a cellular reducing system, efficiently regulates the rate of NO consumption by metabolizing NO in an O2-dependent manner with decreased NO consumption in physiological hypoxia. Here we show that Cygb is a major regulator of NO degradation and cardiovascular tone...
April 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28258342/col1a1-prpf40a-and-ucp2-correlate-with-hypoxia-markers-in-non-small-cell-lung-cancer
#17
Urszula Oleksiewicz, Triantafillos Liloglou, Kalliopi-Maria Tasopoulou, Nikoleta Daskoulidou, John R Gosney, John K Field, George Xinarianos
PURPOSE: Collagen 1A1 (COL1A1), RNA-binding and pre-mRNA Processing Factor (PRPF40A), and Uncoupling Protein 2 (UCP2) were identified as downstream effectors of cytoglobin (CYGB), which was shown implicated in tumour biology. Although these three genes have been previously associated with cancer, little is known about their status in lung malignancies. METHODS: Hereby, we investigated the expression and promoter methylation of COL1A1, PRPF40A, and UCP2 in 156 non-small cell lung cancer (NSCLC) and adjacent normal tissues...
July 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28218331/ultrafast-photochemistry-of-the-bc1-complex
#18
Marten H Vos, Brandon J Reeder, Fevzi Daldal, Ursula Liebl
We present a full investigation of ultrafast light-induced events in the membraneous cytochrome bc1 complex by transient absorption spectroscopy. This energy-transducing complex harbors four redox-active components per monomer: heme c1, two 6-coordinate b-hemes and a [2Fe-2S] cluster. Using excitation of these components in different ratios under various excitation conditions, probing in the full visible range and under three well-defined redox conditions, we demonstrate that for all ferrous hemes of the complex photodissociation of axial ligands takes place and that they rebind in 5-7 ps, as in other 6-coordinate heme proteins, including cytoglobin, which is included as a reference in this study...
March 1, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28157235/possible-involvement-of-nitric-oxide-in-enhanced-liver-injury-and-fibrogenesis-during-cholestasis-in-cytoglobin-deficient-mice
#19
Tuong Thi Van Thuy, Le Thi Thanh Thuy, Katsutoshi Yoshizato, Norifumi Kawada
This study clarified the role of Cygb, the fourth globin in mammals originally discovered in rat hepatic stellate cells (HSCs), in cholestatic liver disease. Bile duct ligation (BDL) augmented inflammatory reactions as revealed by increased infiltrating neutrophils, CD68(+)-macrophages, and chemokine expression in Cygb(-/-) mice. In these mice, impairment of bile canalicular indicated by the loss of CD10 expression, down-regulation of bile salt transporters, increased total bile acid, and massive apoptotic and necrotic hepatocytes occurred with the release of cytochrome c, activation of caspase 3, resulting in reduced animal survival compared to wild-type mice...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28128325/protective-effects-of-recombinant-human-cytoglobin-against-chronic-alcohol-induced-liver-disease-in-vivo-and-in-vitro
#20
Jian Wen, Yongbin Wu, Wei Wei, Zhen Li, Ping Wang, Shiwei Zhu, Wenqi Dong
Alcoholic liver disease (ALD) is an important worldwide public health issue with no satisfying treatment available since now. Here we explore the effects of recombinant human cytoglobin (rhCygb) on chronic alcohol-induced liver injury and the underlying mechanisms. In vivo studies showed that rhCygb was able to ameliorate alcohol-induced liver injury, significantly reversed increased serum index (ALT, AST, TG, TC and LDL-C) and decreased serum HDL-C. Histopathology observation of the liver of rats treated with rhCygb confirmed the biochemical data...
January 27, 2017: Scientific Reports
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