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https://www.readbyqxmd.com/read/29645029/regulation-of-both-the-structure-and-function-by-a-de-novo-designed-disulfide-bond-a-case-study-of-heme-proteins-in-myoglobin
#1
Lu-Lu Yin, Hong Yuan, Ke-Jie Du, Bo He, Shu-Qin Gao, Ge-Bo Wen, Xiangshi Tan, Ying-Wu Lin
A de novo designed intramolecular disulfide bond in myoglobin, resembling that in cytoglobin without structural evidence, was confirmed by an X-ray structure for the first time and was demonstrated to regulate both the structure and function of this protein, which fulfills the design of an artificial dehaloperoxidase, with an activity exceeding that of a native enzyme.
April 12, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29534101/stagnation-of-histopathological-improvement-is-a-predictor-of-hepatocellular-carcinoma-development-after-hepatitis-c-virus-eradication
#2
Hiroyuki Motoyama, Akihiro Tamori, Shoji Kubo, Sawako Uchida-Kobayashi, Shigekazu Takemura, Shogo Tanaka, Satoko Ohfuji, Yuga Teranishi, Ritsuzo Kozuka, Etsushi Kawamura, Atsushi Hagihara, Hiroyasu Morikawa, Masaru Enomoto, Yoshiki Murakami, Norifumi Kawada
BACKGROUND: Hepatocellular carcinoma (HCC) develops in some patients who achieve sustained virological response (SVR) against hepatitis C virus (HCV) infection via anti-HCV therapy. To examine the pathogenesis of HCC development after HCV eradication, histopathological changes and clinical markers were evaluated in SVR patients. METHODS: Of 654 SVR patients treated with interferon (IFN)-based therapies, 34 patients who had undergone liver biopsy before initiating IFN therapy and after SVR achievement were enrolled: 11 patients with HCC and 23 patients without HCC (male/female, 9/2 and 8/15, respectively: age, 58 ± 5 and 54 ± 11 years, respectively)...
2018: PloS One
https://www.readbyqxmd.com/read/29523849/p63-is-a-key-regulator-of-irhom2-signalling-in-the-keratinocyte-stress-response
#3
Paola Arcidiacono, Catherine M Webb, Matthew A Brooke, Huiqing Zhou, Paul J Delaney, Keat-Eng Ng, Diana C Blaydon, Andrew Tinker, David P Kelsell, Anissa Chikh
Hyperproliferative keratinocytes induced by trauma, hyperkeratosis and/or inflammation display molecular signatures similar to those of palmoplantar epidermis. Inherited gain-of-function mutations in RHBDF2 (encoding iRHOM2) are associated with a hyperproliferative palmoplantar keratoderma and squamous oesophageal cancer syndrome (termed TOC). In contrast, genetic ablation of rhbdf2 in mice leads to a thinning of the mammalian footpad, and reduces keratinocyte hyperproliferation and migration. Here, we report that iRHOM2 is a novel target gene of p63 and that both p63 and iRHOM2 differentially regulate cellular stress-associated signalling pathways in normal and hyperproliferative keratinocytes...
March 9, 2018: Nature Communications
https://www.readbyqxmd.com/read/29503620/oxidative-stress-and-renal-fibrosis-recent-insights-for-the-development-of-novel-therapeutic-strategies
#4
REVIEW
Wenshan Lv, George W Booz, Fan Fan, Yangang Wang, Richard J Roman
Chronic kidney disease (CKD) is a significant worldwide healthcare problem. Regardless of the initial injury, renal fibrosis is the common final pathway leading to end stage renal disease. Although the underlying mechanisms are not fully defined, evidence indicates that besides inflammation, oxidative stress plays a crucial role in the etiology of renal fibrosis. Oxidative stress results from an imbalance between the production of free radicals that are often increased by inflammation and mitochondrial dysfunction, and reduced anti-oxidant defenses...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29278960/maternal-cytoglobin-cygb-serum-levels-in-normal-and-preeclamptic-pregnancies
#5
Ahter Tanay Tayyar, Ahmet Tayyar, Sukran Kozali, Resul Karakus, Seren Karakus, Ilkbal Temel Yuksel, Ismail Dag, Gonca Yetkin Yildirim, Oya Demirci
OBJECTIVE: To investigate cytoglobin levels in women with preeclampsia and women with uncomplicated pregnancies. MATERIALS AND METHODS: A cross-sectional study including 26 pregnant women complicated with early-onset preeclampsia (EO-PE) and 26 pregnant women complicated with late-onset preeclampsia (LO-PE) were recruited for the study group. Twenty-seven healthy pregnant women selected randomly were included in the control group. The serum CYGB concentrations were measured using an enzyme-linked immunosorbent assay...
January 7, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29155105/functional-diversification-of-sea-lamprey-globins-in-evolution-and-development
#6
Angela Fago, Kim Rohlfing, Elin E Petersen, Agnieszka Jendroszek, Thorsten Burmester
Agnathans have a globin repertoire that markedly differs from that of jawed (gnathostome) vertebrates. The sea lamprey (Petromyzon marinus) harbors at least 18 hemoglobin, two myoglobin, two globin X, and one cytoglobin genes. However, agnathan hemoglobins and myoglobins are not orthologous to their cognates in jawed vertebrates. Thus, blood-based O2 transport and muscle-based O2 storage proteins emerged twice in vertebrates from a tissue-globin ancestor. Notably, the sea lamprey displays three switches in hemoglobin expression in its life cycle, analogous to hemoglobin switching in vertebrates...
February 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29149638/roles-of-n-and-c-terminal-domains-in-the-ligand-binding-properties-of-cytoglobin
#7
Shumpei Hanai, Hirofumi Tsujino, Taku Yamashita, Ryo Torii, Hitomi Sawai, Yoshitsugu Shiro, Koji Oohora, Takashi Hayashi, Tadayuki Uno
Cytoglobin (Cygb) is a member of the hexacoordinated globin protein family and is expressed ubiquitously in rat and human tissues. Although Cygb is reportedly upregulated under hypoxic conditions both in vivo and in vitro, suggesting a physiological function to protect cells under hypoxic/ischemic conditions by scavenging reactive oxygen species or by signal transduction, the mechanisms associated with this function have not been fully elucidated. Recent studies comparing Cygbs among several species suggest that mammalian Cygbs show a distinctly longer C-terminal domain potentially involved in unique physiological functions...
February 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29128400/strong-modulation-of-nitrite-reductase-activity-of-cytoglobin-by-disulfide-bond-oxidation-implications-for-nitric-oxide-homeostasis
#8
Brandon J Reeder, John Ukeri
Globin-mediated nitric oxide (NO) dioxygenase and nitrite reductase activities have been proposed to serve protective functions within the cell by scavenging or generating NO respectively. Cytoglobin has rapid NO dioxygenase activity, similar to other globins, however, the apparent rates of nitrite reductase activity have been reported as slow or negligible. Here we report that the activity of cytoglobin nitrite reductase activity is strongly dependent on the oxidation state of the two surface-exposed cysteine residues...
January 30, 2018: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28954806/cytoglobin-at-the-crossroads-of-vascular-remodeling
#9
EDITORIAL
Matthew B Amdahl, Anthony W DeMartino, Jesús Tejero, Mark T Gladwin
No abstract text is available yet for this article.
October 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28954528/recombinant-human-cytoglobin-prevents-atherosclerosis-by-regulating-lipid-metabolism-and-oxidative-stress
#10
Lingling Ou, Xin Li, Baihong Chen, Zhenhuang Ge, Junyi Zhang, Ye Zhang, Gaotai Cai, Zhen Li, Ping Wang, Wenqi Dong
Atherosclerosis is a chronic inflammatory vascular disease characterized by lipid accumulation and endothelial dysfunction. Cytoglobin has been shown to exert protective effects under oxidative stress conditions. The aim of this study was to determine whether recombinant human cytoglobin (rhCYGB) has protective effects against atherosclerosis. We intraperitoneally injected rhCYGB (0, 4, or 7 mg/kg BW) into the atherosclerotic rats daily for 60 days. The rhCYGB injections reduced low-density lipoprotein cholesterol (LDL-C) levels and increased high-density lipoprotein cholesterol levels in a dose-dependent manner, rhCYGB (7 mg/kg) significantly attenuated atherosclerosis...
January 1, 2017: Journal of Cardiovascular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28929379/erratum-to-anatomical-specificity-of-the-brain-in-the-modulation-of-neuroglobin-and-cytoglobin-genes-after-chronic-bisphenol-a-exposure
#11
Rodrigo Rodrigues da Conceição, Janaina Sena de Souza, Kelen Carneiro de Oliveira, Rui Monteiro de Barros Maciel, Marco Aurélio Romano, Renata Marino Romano, Magnus Régios Dias da Silva, Maria Izabel Chiamolera, Gisele Giannocco
No abstract text is available yet for this article.
December 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28918749/effect-of-low-temperature-on-globin-expression-respiratory-metabolic-enzyme-activities-and-gill-structure-of-litopenaeus-vannamei
#12
Meng Wu, Nan Chen, Chun-Xiao Huang, Yan He, Yong-Zhen Zhao, Xiao-Han Chen, Xiu-Li Chen, Huan-Ling Wang
Low temperature frequently influences growth, development, and even survival of aquatic animals. In the present study, physiological and molecular responses to low temperature in Litopenaeus vannamei were investigated. The cDNA sequences of two oxygen-carrying proteins, cytoglobin (Cygb) and neuroglobin (Ngb), were isolated. Protein structure analysis revealed that both proteins share a globin superfamily domain. Real-time PCR analysis indicated that Cygb and Ngb mRNA levels gradually increased during decrease in temperatures from 25 to 15°C and then decreased at 10°C in muscle, brain, stomach, and heart, except for a continuing increase in gills, whereas they showed a different expression trend in the hepatopancreas...
July 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28916723/fibroblast-growth-factor-2-fgf2-regulates-cytoglobin-expression-and-activation-of-human-hepatic-stellate-cells-via-jnk-signaling
#13
Misako Sato-Matsubara, Tsutomu Matsubara, Atsuko Daikoku, Yoshinori Okina, Lisa Longato, Krista Rombouts, Le Thi Thanh Thuy, Jun Adachi, Takeshi Tomonaga, Kazuo Ikeda, Katsutoshi Yoshizato, Massimo Pinzani, Norifumi Kawada
Cytoglobin (CYGB) belongs to the mammalian globin family and is exclusively expressed in hepatic stellate cells (HSCs) in the liver. In addition to its gas-binding ability, CYGB is relevant to hepatic inflammation, fibrosis, and cancer because of its anti-oxidative properties; however, the regulation of CYGB gene expression remains unknown. Here, we sought to identify factors that induce CYGB expression in HSCs and to clarify the molecular mechanism involved. We used the human HSC cell line HHSteC and primary human HSCs isolated from intact human liver tissues...
November 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28883470/cytoglobin-promotes-cardiac-progenitor-cell-survival-against-oxidative-stress-via-the-upregulation-of-the-nf%C3%AE%C2%BAb-inos-signal-pathway-and-nitric-oxide-production
#14
Shuning Zhang, Xiuchun Li, Frances L Jourd'heuil, Shunlin Qu, Neil Devejian, Edward Bennett, David Jourd'heuil, Chuanxi Cai
Human cardiac stem/progenitor cells (hCPCs) may serve in regenerative medicine to repair the infarcted heart. However, this approach is severely limited by the poor survival of donor cells. Recent studies suggest that the mammalian globin cytoglobin (CYGB) regulates nitric oxide (NO) metabolism and cell death. In the present study, we found that CYGB is expressed in hCPCs. Through molecular approaches aimed at increasing or decreasing CYGB expression in hCPCs, we found that CYGB functions as a pro-survival factor in response to oxidative stress...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28798140/the-hemoglobin-homolog-cytoglobin-in-smooth-muscle-inhibits-apoptosis-and-regulates-vascular-remodeling
#15
Frances L Jourd'heuil, Haiyan Xu, Timothy Reilly, Keneta McKellar, Chaymae El Alaoui, Julia Steppich, Yong Feng Liu, Wen Zhao, Roman Ginnan, David Conti, Reynold Lopez-Soler, Arif Asif, Rebecca K Keller, John J Schwarz, Le Thi Thanh Thuy, Norifumi Kawada, Xiaochun Long, Harold A Singer, David Jourd'heuil
OBJECTIVE: The role of hemoglobin and myoglobin in the cardiovascular system is well established, yet other globins in this context are poorly characterized. Here, we examined the expression and function of cytoglobin (CYGB) during vascular injury. APPROACH AND RESULTS: We characterized CYGB content in intact vessels and primary vascular smooth muscle (VSM) cells and used 2 different vascular injury models to examine the functional significance of CYGB in vivo. We found that CYGB was strongly expressed in medial arterial VSM and human veins...
October 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28721559/anatomical-specificity-of-the-brain-in-the-modulation-of-neuroglobin-and-cytoglobin-genes-after-chronic-bisphenol-a-exposure
#16
Rodrigo Rodrigues da Conceição, Janaina Sena de Souza, Kelen Carneiro de Oliveira, Rui Monteiro de Barros Maciel, Marco Aurélio Romano, Renata Marino Romano, Magnus Régios Dias da Silva, Maria Izabel Chiamolera, Gisele Giannocco
The aim of this study was to investigate the influence of Bisphenol A (BPA) exposure on Neuroglobin (Ngb) and Cytoglobin (Cygb) as well as oxidative stress gene expression in the cerebellum, hippocampus, hypothalamus and cortex. Male Wistar rats were randomly divided into 3 groups: Control and two groups receiving 2 different daily BPA dosages, 5 or 25 mg/kg from postnatal day 50 (PND50) through PND90 and they were euthanized at PND105. In the cortex, we found an increase in Ngb gene expression and also in superoxide dismutase 1 and Catalase (Cat)...
December 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28671819/efficient-reduction-of-vertebrate-cytoglobins-by-the-cytochrome-b-5-cytochrome-b-5-reductase-nadh-system
#17
COMPARATIVE STUDY
Matthew B Amdahl, Courtney E Sparacino-Watkins, Paola Corti, Mark T Gladwin, Jesús Tejero
Cytoglobin is a heme-containing protein ubiquitous in mammalian tissues. Unlike the evolutionarily related proteins hemoglobin and myoglobin, cytoglobin shows a six-coordinated heme binding, with the heme iron coordinated by two histidine side chains. Cytoglobin is involved in cytoprotection pathways through yet undefined mechanisms, and it has recently been demonstrated that cytoglobin has redox signaling properties via nitric oxide (NO) and nitrite metabolism. The reduced, ferrous cytoglobin can bind oxygen and will react with NO in a dioxygenation reaction to form nitrate, which dampens NO signaling...
August 1, 2017: Biochemistry
https://www.readbyqxmd.com/read/28662027/the-different-expressed-serum-proteins-in-rhcygb-treated-rat-model-of-liver-fibrosis-by-the-optimized-two-dimensional-gel-electrophoresis
#18
Gaotai Cai, Bohong Chen, Zhen Li, Wei Wei, Ping Wang, Wenqi Dong
Liver fibrosis, a common pathological process of chronic liver diseases, is the final stage of liver dysfunction that has severely deleterious impact on human health. Cytoglobin was first discovered in 2001 by proteomic analysis in rat stellate cells and was reported to play an important role in controlling tissue fibrosis. However, the mechanism by which cytoglobin inhibits or reverses the progression of fibrosis remains unclear. The present study examines the effect of recombinant human cytoblobin (rhCygb) in a rat model of liver fibrosis...
2017: PloS One
https://www.readbyqxmd.com/read/28620820/cytoglobin-inhibits-migration-through-pi3k-akt-mtor-pathway-in-fibroblast-cells
#19
Selami Demirci, Ayşegül Doğan, Hüseyin Apdik, Emre Can Tuysuz, Sukru Gulluoglu, Omer Faruk Bayrak, Fikrettin Şahin
Cell proliferation and migration are crucial in many physiological processes including development, cancer, tissue repair, and wound healing. Cell migration is regulated by several signaling molecules. Identification of genes related to cell migration is required to understand molecular mechanism of non-healing chronic wounds which is a major concern in clinics. In the current study, the role of cytoglobin (CYGB) gene in fıbroblast cell migration and proliferation was described. L929 mouse fibroblast cells were transduced with lentiviral particles for CYGB and GFP, and analyzed for cell proliferation and migration ability...
January 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28593139/oxygen-binding-and-nitric-oxide-dioxygenase-activity-of-cytoglobin-are-altered-to-different-extents-by-cysteine-modification
#20
Danlei Zhou, Craig Hemann, James Boslett, Aiqin Luo, Jay L Zweier, Xiaoping Liu
Cytoglobin (Cygb), like other members of the globin family, is a nitric oxide (NO) dioxygenase, metabolizing NO in an oxygen (O2)-dependent manner. We examined the effect of modification of cysteine sulfhydryl groups of Cygb on its O2 binding and NO dioxygenase activity. The two cysteine sulfhydryls of Cygb were modified to form either an intramolecular disulfide bond (Cygb_SS), thioether bonds to N-ethylmaleimide (NEM; Cygb_SC), or were maintained as free SH groups (Cygb_SH). It was observed that the NO dioxygenase activity of Cygb only slightly changed (~ 25%) while the P50 of O2 binding to Cygb changed over four-fold with these modifications...
June 2017: FEBS Open Bio
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