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https://www.readbyqxmd.com/read/29134302/nuclear-organization-during-in-vitro-differentiation-of-porcine-mesenchymal-stem-cells-mscs-into-adipocytes
#1
Joanna Stachecka, Agnieszka Walczak, Beata Kociucka, Błażej Ruszczycki, Grzegorz Wilczyński, Izabela Szczerbal
Differentiation of progenitor cells into adipocytes is accompanied by remarkable changes in cell morphology, cytoskeletal organization, and gene expression profile. Mature adipocytes are filled with a large lipid droplet and the nucleus tends to move to the cell periphery. It was hypothesized that the differentiation process is also associated with changes of nuclear organization. The aim of this study was to determine the number and distribution of selected components of nuclear architecture during porcine in vitro adipogenesis...
November 13, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29127365/the-primary-cilium-is-necessary-for-the-differentiation-and-the-maintenance-of-human-adipose-progenitors-into-myofibroblasts
#2
N Arrighi, K Lypovetska, C Moratal, S Giorgetti-Peraldi, C A Dechesne, C Dani, P Peraldi
The primary cilium is an organelle, present at the cell surface, with various biological functions. We, and others, have shown that it plays a role in the differentiation of adipose progenitors (APs) into adipocytes. APs can also differentiate into myofibroblasts when treated with TGF-β1. Several components of the TGF-β1 pathway are located within the cilium suggesting a function for this organelle in AP myofibrogenesis. We studied differentiation of APs into myofibroblasts in two human models: APs of the adipose tissue (aAPs) and APs resident in the skeletal muscles (mAPs)...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29125884/comparative-characterization-of-cd271-and-cd271-subpopulations-of-cd34-human-adipose-derived-stromal-cells
#3
Liziane Raquel Beckenkamp, Lucas Eduardo Botelho Souza, Fernanda Ursoli Ferreira Melo, Carolina Hassibe Thomé, Daniele Aparecida Rosa Magalhães, Patrícia Viana Bonini Palma, Dimas Tadeu Covas
INTRODUCTION: Adipose-derived stromal/stem cells (ASCs) are promising candidates for cell-based therapies. However, the lack of markers able to unequivocally identify these cells, the differential expression of cell surface molecules among stromal progenitors from different tissues and cellular alterations caused by culture are phenomena that need to be comprehensively addressed in order to improve ASC purification and consequently refine our knowledge about their function and therapeutic efficiency...
November 10, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29120093/imbalanced-osteogenesis-and-adipogenesis-in-mice-deficient-of-the-chemokine-cxcl12-sdf1-in-the-bone-mesenchymal-stem-progenitor-cells
#4
Yi-Shiuan Tzeng, Ni-Chun Chung, Yu-Ren Chen, Hsin-Yi Huang, Wen-Po Chunang, Dar-Ming Lai
Bone and bone marrow serve as an imperative ecosystem to various types of cells participating in critical body functions. The chemokine Cxcl12, also known as stromal cell-derived factor 1 (Sdf1), is one of the communication factors in the marrow microenvironment that regulate hematopoietic stem/progenitor cell homeostasis. However, the function of Cxcl12 in other bone marrow cells in vivo is yet to be discovered. Here we report a novel function of Cxcl12 in postnatal bone development and homeostasis. Targeted deletion of Cxcl12 in Prx1- or Osx-expressing mesenchymal stem/progenitor cells (MSPCs), but not in mature osteoblasts, resulted in marrow adiposity and reduced trabecular bone content...
November 9, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29116032/single-cell-transcriptomics-suggest-that-human-adipocyte-progenitor-cells-constitute-a-homogeneous-cell-population
#5
Juan R Acosta, Simon Joost, Kasper Karlsson, Anna Ehrlund, Xidan Li, Myriam Aouadi, Maria Kasper, Peter Arner, Mikael Rydén, Jurga Laurencikiene
Regulation of adipose tissue stem cells (ASCs) and adipogenesis impact the development of excess body fat-related metabolic complications. Animal studies have suggested the presence of distinct subtypes of ASCs with different differentiation properties. In addition, ASCs are becoming the biggest source of mesenchymal stem cells used in therapies, which requires deep characterization. Using unbiased single cell transcriptomics we aimed to characterize ASC populations in human subcutaneous white adipose tissue (scWAT)...
November 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29113460/impact-of-age-on-human-adipose-stem-cells-for-bone-tissue-engineering
#6
Denis Dufrane
Bone nonunion is a pathological condition in which all bone healing processes have stopped, resulting in abnormal mobility between 2 bone segments. The incidence of bone-related injuries will increase in an aging population, leading to such injuries reaching epidemic proportions. Tissue engineering and cell therapy using mesenchymal stem cells (MSCs) have raised the possibility of implanting living tissue for bone reconstruction. Bone marrow was first proposed as the source of stem cells for bone regeneration...
September 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29102342/bioengineered-lungs-a-challenge-and-an-opportunity
#7
Ramon Farré, Jordi Otero, Isaac Almendros, Daniel Navajas
Lung biofabrication is a new tissue engineering and regenerative development aimed at providing organs for potential use in transplantation. Lung biofabrication is based on seeding cells into an acellular organ scaffold and on culturing them in an especial purpose bioreactor. The acellular lung scaffold is obtained by decellularizing a non-transplantable donor lung by means of conventional procedures based on application of physical, enzymatic and detergent agents. To avoid immune recipient's rejection of the transplanted bioengineered lung, autologous bone marrow/adipose tissue-derived mesenchymal stem cells, lung progenitor cells or induced pluripotent stem cells are used for biofabricating the bioengineered lung...
November 1, 2017: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/29079730/engineering-of-an-angiogenic-niche-by-perfusion-culture-of-adipose-derived-stromal-vascular-fraction-cells
#8
Giulia Cerino, Emanuele Gaudiello, Manuele Giuseppe Muraro, Friedrich Eckstein, Ivan Martin, Arnaud Scherberich, Anna Marsano
In vitro recapitulation of an organotypic stromal environment, enabling efficient angiogenesis, is crucial to investigate and possibly improve vascularization in regenerative medicine. Our study aims at engineering the complexity of a vascular milieu including multiple cell-types, a stromal extracellular matrix (ECM), and molecular signals. For this purpose, the human adipose stromal vascular fraction (SVF), composed of a heterogeneous mix of pericytes, endothelial/stromal progenitor cells, was cultured under direct perfusion flow on three-dimensional (3D) collagen scaffolds...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29068928/the-effect-of-processing-technique-on-fat-graft-survival
#9
COMPARATIVE STUDY
Orlando Canizares, Jennifer E Thomson, Robert J Allen, Edward H Davidson, John P Tutela, Pierre B Saadeh, Stephen M Warren, Alexes Hazen
BACKGROUND: Wide variations in fat graft survival have been reported. The authors hypothesize that treating the adipose tissue on Telfa gauze creates a processed lipoaspirate with a more functional adipokine profile that improves fat graft survival. METHODS: Suction-assisted lipoaspirate was harvested from humans and was either processed by centrifugation, rolled on Telfa gauze, or left unprocessed. Progenitor cell populations were quantified and characterized by flow cytometry...
November 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29050915/decellularized-matrix-of-adipose-derived-mesenchymal-stromal-cells-enhanced-retinal-progenitor-cell-proliferation-via-the-akt-erk-pathway-and-neuronal-differentiation
#10
Jing Ji, Dandan Zhang, Wei Wei, Bingqiao Shen, Yi Zhang, Yuyao Wang, Zhimin Tang, Ni Ni, Hao Sun, Jiaqiang Liu, Xianqun Fan, Ping Gu
BACKGROUND AIMS: Retinal progenitor cells (RPCs) are a promising cell therapy treatment for retinal degenerative diseases. However, problems with limited proliferation ability and differentiation preference toward glia rather than neurons restrict the clinical application of these RPCs. The extracellular matrix (ECM) has been recognized to provide an appropriate microenvironment to support stem cell adhesion and direct cell behaviors, such as self-renewal and differentiation. METHODS: In this study, decellularized matrix of adipose-derived mesenchymal stromal cells (DMA) was manufactured using a chemical agent method (0...
October 16, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29038672/safety-analysis-of-autologous-stem-cell-therapy-in-a-variety-of-degenerative-diseases-and-injuries-using-the-stromal-vascular-fraction
#11
Kristin Comella, Michelle Parlo, Rosemary Daly, Vincent Depasquale, Eric Edgerton, Patrick Mallory, Roy Schmidt, Walter P Drake
BACKGROUND: Stem cells from adipose tissue offer a novel therapy for patients with damaged tissue. Stromal vascular fraction (SVF) injected into patients may reduce inflammation, promote healing, and repair damaged/scarred tissue. SVF can be isolated from fat (adipose) tissue in an outpatient procedure. The SVF population includes mesenchymal stem cells (MSCs), pericytes, endothelial/progenitor cells, fibroblasts and growth factors where the adipocyte (fat cell) population has been removed...
November 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/29027120/elucidating-the-preadipocyte-and-its-role-in-adipocyte-formation-a-comprehensive-review
#12
REVIEW
Christos N Sarantopoulos, Derek A Banyard, Mary E Ziegler, Beatrice Sun, Ashkaun Shaterian, Alan D Widgerow
Adipogenesis is a complex process whereby the multipotent adipose-derived stem cell is converted to a preadipocyte before terminal differentiation into the mature adipocyte. Preadipocytes are present throughout adult life, exhibit adipose fat depot specificity, and differentiate and proliferate from distinct progenitor cells. The mechanisms that promote preadipocyte commitment and maturation involve numerous protein factor regulators, epigenetic factors, and miRNAs. Detailed characterization of this process is currently an area of intense research and understanding the roles of preadipocytes in tissue plasticity may provide insight into novel approaches for tissue engineering, regenerative medicine and treating a host of obesity-related conditions...
October 13, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/29025212/use-of-freshly-isolated-human-adipose-stromal-cells-for-clinical-applications
#13
Ryan A Lockhart, Joel A Aronowitz, Severiano Dos-Anjos Vilaboa
The clinical use of adipose-derived cells is being explored very actively around the world for various human diseases. Adipose tissue is an abundant tissue source that can be easily harvested using liposuction. Human lipoaspirates contain a significant amount of mesenchymal stromal cells, as well as other progenitors and terminally differentiated cell types. This review covers the isolation of adipose stromal vascular fraction (SVF), the quality control and safety analysis of freshly isolated cell suspensions...
July 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29023156/stem-cell-therapy-for-articular-cartilage-repair-review-of-the-entity-of-cell-populations-used-and-the-result-of-the-clinical-application-of-each-entity
#14
Yong-Beom Park, Chul-Won Ha, Ji Heon Rhim, Han-Jun Lee
BACKGROUND: Following successful preclinical studies, stem cell therapy is emerging as a candidate for the treatment of articular cartilage lesions. Because stem cell therapy for cartilage repair in humans is at an early phase, confusion and errors are found in the literature regarding use of the term stem cell therapy in this field. PURPOSE: To provide an overview of the outcomes of cartilage repair, elucidating the various cell populations used, and thus reduce confusion with regard to using the term stem cell therapy...
October 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29022261/the-non-cardiomyocyte-cells-of-the-heart-their-possible-roles-in-exercise-induced-cardiac-regeneration-and-remodeling
#15
Ivan Varga, Jan Kyselovič, Paulina Galfiova, Lubos Danisovic
The non-cardiomyocyte cellular microenvironment of the heart includes diverse types of cells of mesenchymal origin. During development, the majority of these cells derive from the epicardium, while a subset derives from the endothelium/endocardium and neural crest derived mesenchyme. This subset includes cardiac fibroblasts and telocytes, the latter of which are a controversial type of "connecting cell" that support resident cardiac progenitors in the postnatal heart. Smooth muscle cells, pericytes, and endothelial cells are also present, in addition to adipocytes, which accumulate as epicardial adipose connective tissue...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29021914/retinoic-acid-induces-white-adipose-tissue-browning-by-increasing-adipose-vascularity-and-inducing-beige-adipogenesis-of-pdgfr%C3%AE-adipose-progenitors
#16
Bo Wang, Xing Fu, Xingwei Liang, Jeanene M Deavila, Zhixiu Wang, Liang Zhao, Qiyu Tian, Junxing Zhao, Noe Alberto Gomez, Sophie C Trombetta, Mei-Jun Zhu, Min Du
Formation of beige adipocytes within white adipose tissue enhances energy expenditure, which is a promising strategy to reduce obesity and prevent metabolic symptoms. Vitamin A and its bioactive metabolite, retinoic acid (RA), have regulatory roles in lipid metabolism. Here we report that RA induces white adipose tissue browning via activating vascular endothelial growth factor (VEGF) signaling. RA triggered angiogenesis and elicited de novo generation of platelet-derived growth factor receptor α positive (PDGFRα(+)) adipose precursor cells via VEGFA/VEGFR2 signaling...
2017: Cell Discovery
https://www.readbyqxmd.com/read/29020119/human-adipose-derived-stem-cells-support-the-growth-of-limbal-stem-progenitor-cells
#17
Hua Mei, Sheyla González, Martin N Nakatsu, Elfren R Baclagon, Felix V Chen, Sophie X Deng
The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro. To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D)...
2017: PloS One
https://www.readbyqxmd.com/read/29018484/infrapatellar-fat-pad-stem-cells-from-developmental-biology-to-cell-therapy
#18
REVIEW
Ronaldo J F C do Amaral, Henrique V Almeida, Daniel J Kelly, Fergal J O'Brien, Cathal J Kearney
The ideal cell type to be used for cartilage therapy should possess a proven chondrogenic capacity, not cause donor-site morbidity, and should be readily expandable in culture without losing their phenotype. There are several cell sources being investigated to promote cartilage regeneration: mature articular chondrocytes, chondrocyte progenitors, and various stem cells. Most recently, stem cells isolated from joint tissue, such as chondrogenic stem/progenitors from cartilage itself, synovial fluid, synovial membrane, and infrapatellar fat pad (IFP) have gained great attention due to their increased chondrogenic capacity over the bone marrow and subcutaneous adipose-derived stem cells...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28988272/pre-culture-in-endothelial-growth-medium-enhances-the-angiogenic-properties-of-adipose-derived-stem-stromal-cells
#19
Lucas E B Souza, Liziane R Beckenkamp, Lays M Sobral, Daianne M C Fantacini, Fernanda U F Melo, Josiane S Borges, Andréia M Leopoldino, Simone Kashima, Dimas Tadeu Covas
Considerable progress has been made on the development of adipose-derived stem/stromal cells (ASCs) as pro-angiogenic therapeutic tools. However, variable clinical results highlight the need for devising strategies to enhance their therapeutic efficacy. Since ASCs proliferate and stabilize newly formed vessels during the angiogenic phase of adipose tissue formation, we hypothesized that mimicking an angiogenic milieu during culture of ASCs would enhance their capacity to support endothelial cell survival and angiogenesis...
October 7, 2017: Angiogenesis
https://www.readbyqxmd.com/read/28983600/cd146-skeletal-stem-cells-from-growth-plate-exhibit-specific-chondrogenic-differentiation-capacity-in%C3%A2-vitro
#20
Ying-Xing Wu, Xing-Zhi Jing, Yue Sun, Ya-Ping Ye, Jia-Chao Guo, Jun-Ming Huang, Wei Xiang, Jia-Ming Zhang, Feng-Jing Guo
Skeletal stem cells (SSCs) are a population of progenitor cells which give rise to postnatal skeletal tissues including bone, cartilage and bone marrow stroma, however not to adipose, haematopoietic or muscle tissue. Growth plate chondrocytes exhibit the ability of continuous proliferation and differentiation, which contributes to the continuous physiological growth. The growth plate has been hypothesized to contain SSCs which exhibit a desirable differentiation capacity to generate bone and cartilage. Due to the heterogeneity of the growth plate chondrocytes, SSCs in the growth plate are not well studied...
December 2017: Molecular Medicine Reports
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