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https://www.readbyqxmd.com/read/28529666/a-microfluidic-model-of-hemostasis-sensitive-to-platelet-function-and-coagulation
#1
R M Schoeman, K Rana, N Danes, M Lehmann, J A Di Paola, A L Fogelson, K Leiderman, K B Neeves
Hemostasis is the process of sealing a vascular injury with a thrombus to arrest bleeding. The type of thrombus that forms depends on the nature of the injury and hemodynamics. There are many models of intravascular thrombus formation whereby blood is exposed to prothrombotic molecules on a solid substrate. However, there are few models of extravascular thrombus formation whereby blood escapes into the extravascular space through a hole in the vessel wall. Here, we describe a microfluidic model of hemostasis that includes vascular, vessel wall, and extravascular compartments...
February 2017: Cellular and Molecular Bioengineering
https://www.readbyqxmd.com/read/28516356/morphology-ultrastructure-and-element-uptake-in-calophyllum-brasiliense-cambess-calophyllaceae-j-agardh-seedlings-under-cadmium-exposure
#2
Alezania Silva Pereira, Priscila Andressa Cortez, Alex-Alan Furtado de Almeida, Majeti Narashima Vara Prasad, Marcel Giovanni Costa França, Maura da Cunha, Raildo Mota de Jesus, Pedro Antônio Oliveira Mangabeira
Cadmium (Cd) is a metal known for its genotoxicity and cytotoxicity, much concerned for its potential environmental and human health impacts. This study evaluates the toxic effect of Cd in Calophyllum brasiliense plants. The plants were cultivated for 30 days in full nutrient solution in order to adapt, and for 15 days in nutrient solution without Cd or with 4, 8, 16, and 32 μmol Cd L(-1). Anatomical analysis of the leaf showed no significant effects of Cd on epidermal thickness in abaxial and adaxial sides, palisade, and spongy parenchyma...
May 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28500525/ftsz-constriction-force-curved-protofilaments-bending-membranes
#3
Harold P Erickson, Masaki Osawa
FtsZ assembles in vitro into protofilaments (pfs) that are one subunit thick and ~50 subunits long. In vivo these pfs assemble further into the Z ring, which, along with accessory division proteins, constricts to divide the cell. We have reconstituted Z rings in liposomes in vitro, using pure FtsZ that was modified with a membrane targeting sequence to directly bind the membrane. This FtsZ-mts assembled Z rings and constricted the liposomes without any accessory proteins. We proposed that the force for constriction was generated by a conformational change from straight to curved pfs...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28492354/experimental-evaluation-of-the-optimal-suture-pattern-with-a-flexible-endoscopic-suturing-system
#4
Peter Halvax, Michele Diana, Yoshihiro Nagao, Jacques Marescaux, Lee Swanström
BACKGROUND: The ability to perform reliable, secure endoluminal closure of the gastrointestinal tract wall, is a prerequisite to support the progress of the emerging field of endoluminal surgery. Along with advanced clipping systems, flexible endoscopic suturing devices are commercially available. Current systems can replicate traditional surgical suturing patterns in the endoluminal environment. The aim of this study was to evaluate the optimal endoluminal suturing technique using a flexible endoscopic suturing device...
June 2017: Surgical Innovation
https://www.readbyqxmd.com/read/28491879/listeria-endocarditis-a-diagnostic-challenge
#5
Wilhelmina J A R M Valckx, Suzanne P M Lutgens, Hortence E Haerkens-Arends, Peter C Barneveld, Jaap J Beutler, Ellen K Hoogeveen
A 74-year-old hemodialysis patient with a history of an atrial septum defect closure, coronary bypass surgery, and a St. Jude aortic prosthetic valve was diagnosed with pneumonia and volume overload. Blood cultures were positive for Listeria monocytogenes, and amoxicillin was given for 2 weeks. Immediately after discontinuation of amoxicillin, fever relapsed. Transthoracic and transesophageal echocardiography showed no sign of endocarditis. Given the fever relapse and 3 positive minor Duke criteria, an (18)F-FDG PET-CT scan ((18)F-fluorodeoxyglucose-positron emission tomography-computed tomography) scan was performed...
April 2017: Journal of Investigative Medicine High Impact Case Reports
https://www.readbyqxmd.com/read/28488072/modified-components-separation-technique-experience-treating-large-complex-ventral-hernias-at-a-university-hospital
#6
A Torregrosa-Gallud, J Sancho Muriel, J Bueno-Lledó, P García Pastor, J Iserte-Hernandez, S Bonafé-Diana, O Carreño-Sáenz, F Carbonell-Tatay
BACKGROUND: An increasing number of patients have large or complex abdominal wall defects. Component separation technique (CST) is a very effective method for reconstructing complex midline abdominal wall defects in a manner that restores innervated muscle function without excessive tension. Our goal is to show our results by a modified CST for treating large ventral hernias. MATERIALS AND METHODS: A total of 351 patients with complex ventral hernias have been treated over a 10-year period...
May 9, 2017: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/28480928/differential-flap-dynamics-in-l-d-transpeptidase2-from-mycobacterium-tuberculosis-revealed-by-molecular-dynamics
#7
Zeynab Fakhar, Thavendran Govender, Glenn E M Maguire, Gyanu Lamichhane, Ross C Walker, Hendrik G Kruger, Bahareh Honarparvar
Despite the advances in tuberculosis treatment, TB is still one the most deadly infectious diseases and remains a major global health quandary. Mycobacterium tuberculosis (Mtb) is the only known mycobacterium with a high content of 3→3 crosslinks in the biosynthesis of peptidoglycan, which is negligible in most bacterial species. An Mtb lacking LdtMt2 leads to alteration of the colony morphology and loss of virulence which makes this enzyme an attractive target. Regardless of the vital role of LdtMt2 for cell wall survival, the impact of ligand binding on the dynamics of the β-hairpin flap is still unknown...
May 8, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28479768/laparoscopic-sacrocolpopexy-using-barbed-sutures-for-mesh-fixation-and-peritoneal-closure-a-safe-option-to-reduce-operational-times
#8
Panagiotis Kallidonis, Abdulrahman Al-Aown, Marinos Vasilas, Iason Kyriazis, Vasilis Panagopoulos, Fotini Fligou, Anastasios Athanasopoulos, Bagheri Fariborz, Evangelos Liatsikos, Mehmet Özsoy
INTRODUCTION: Laparoscopic sacrocolpopexy (LSC) has established itself as a safe method for the management of pelvic organ prolapse (POP). Laparoscopic suturing is a time-consuming intraoperative task during LSC. Self-retaining barbed sutures (SBSs) are known to reduce the operative time in laparoscopic cases. The current study aimed to evaluate the efficacy and safety of SBS during the performance of LSC. MATERIALS AND METHODS: Twenty female patients with symptomatic POP were treated with LSC by an expert surgeon...
April 2017: Urology Annals
https://www.readbyqxmd.com/read/28442692/viscocanalostomy-and-canaloplasty-ab-externo-schlemm-s-canal-surgery
#9
Matthias C Grieshaber
Ab externo Schlemm's canal (SC) surgery (e.g., viscocanalostomy and canaloplasty) is a valuable alternative to glaucoma filtration surgery. It targets the abnormally high resistance to outflow in the trabecular meshwork (TM) and reestablishes the physiologic outflow system. In viscocanalostomy, viscoelastic substance is injected to dilate SC, which in turn leads to microdisruptions of the inner wall. In canaloplasty, the additional intracanalicular stent (suture or Stegmann Canal Expander®) keeps the canal patent and enhances the circumferential flow...
2017: Developments in Ophthalmology
https://www.readbyqxmd.com/read/28442685/deep-sclerectomy
#10
Sylvain Roy, André Mermoud
Filtering surgery evolved from the classic trabeculectomy, in which penetration into the anterior chamber is a necessary step, toward nonpenetrating deep sclerectomy. The first procedure presents several serious complications, such as durable hypotony, hyphema, flat anterior chamber, choroidal detachment, endophthalmitis, and surgery-induced cataract. To avoid such drawbacks, a novel nonpenetrating technique was designed to improve the predictability of the intraocular pressure (IOP)-lowering action, while reducing the incidence of the immediate postoperative complications encountered with the penetrating method...
2017: Developments in Ophthalmology
https://www.readbyqxmd.com/read/28441239/traumatic-abdominal-wall-hernia-in-children-by-handlebar-injury-when-to-suspect-scan-and-call-the-surgeon
#11
Victoria Elisa Rinaldi, Mirko Bertozzi, Elisa Magrini, Sara Riccioni, Giuseppe Di Cara, Antonino Appignani
Traumatic abdominal wall hernias (TAWHs) can be defined as a herniation through disrupted musculature and fascia associated with blunt trauma. They are seen in approximately 1% of patients with blunt abdominal trauma. Data on TAWH in the pediatric population are very limited and principally based on case reports and a few case series. Past reports have indicated that the presence of the "handlebar sign" confers an increased risk of internal injury. Concomitant internal injuries are reported with an incidence between 25% and 70%, and occult hernias may also occur and are usually detected only by abdominal computed tomography scan and ultrasonography...
April 24, 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/28435855/first-clinical-experiences-with-a-novel-endoscopic-over-the-scope-clip-system
#12
Marco Dinelli, Barbara Omazzi, Paolo Andreozzi, Nicola Zucchini, Alessandro Redaelli, Gianpiero Manes
We describe our experience with a new over-the-scope clip (OTSC) system (Padlock Clip?) in the treatment of 14 patients. Eight of the 14 patients were treated for closure of gastrointestinal fistulas (n?=?4), iatrogenic gastrointestinal perforations (n?=?2), or hemostasis of post-polypectomy bleeding (n?=?2). The site of clipping was the lower gastrointestinal tract in five patients and the upper gastrointestinal tract in three patients. The clip was successfully delivered in seven out of the eight patients and clinical success was achieved in all patients...
March 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28429087/the-abdoman-an-artificial-abdominal-wall-simulator-for-biomechanical-studies-on-laparotomy-closure-techniques
#13
L F Kroese, J J Harlaar, C Ordrenneau, J Verhelst, G Guérin, F Turquier, R H M Goossens, G-J Kleinrensink, J Jeekel, J F Lange
PURPOSE: Incisional hernia remains a frequent complication after abdominal surgery associated with significant morbidity and high costs. Animal and clinical studies have exhibited some limitations. The purpose of this study was to develop an artificial human abdominal wall (AW) simulator in order to enable investigations on closure modalities. We hypothesized that a physical model of the human AW would give new insight into commonly used suture techniques representing a substantial complement or alternative to clinical and animal studies...
April 20, 2017: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/28424715/temporal-petal-closure-benefits-reproductive-development-of-magnolia-denudata-magnoliaceae-in-early-spring
#14
Liya Liu, Chulan Zhang, Xiangyu Ji, Zhixiang Zhang, Ruohan Wang
The Magnoliaceae shows strong phylogenetic niche conservatism, in which temporal petal closure has been extensively reported. However, it is yet elusive whether temporal petal closure is an idle floral character inherited from their ancestors or an adaptive trait to their habitats. Here, we monitored the process of temporal floral closure and re-opening in a thermogenic plant, Magnolia denudata (Magnoliaceae). Furthermore, we artificially interrupted temporal petal closure and investigated its effects on development of female and male gametophytes...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28415122/p1138cardiac-shear-wave-velocity-in-healthy-individuals
#15
M Strachinaru, M L Geleijnse, J G Bosch, N De Jong, Afw Van Der Steen, B M Van Dalen, H J Vos
Background: The closure of the valves generates shear waves in the heart walls. The propagation velocity of shear waves relates to stiffness. This could potentially be used to estimate the stiffness of the myocardium, with huge potential implications in pathologies characterized by a deterioration of the diastolic properties of the left ventricle. In an earlier phantom study we already validated shear wave tracking with a clinical ultrasound system in cardiac mode. Purpose: In this study we aimed to measure the shear waves velocity in normal individuals...
December 1, 2016: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28411456/urinary-bladder-injury-during-cesarean-delivery-maternal-outcome-from-a-contemporary-large-case-series
#16
Lina Salman, Shachar Aharony, Anat Shmueli, Arnon Wiznitzer, Rony Chen, Rinat Gabbay-Benziv
BACKGROUND: Urinary bladder injury is a rare complication during cesarean delivery. Little is known on maternal outcome following this injury. OBJECTIVE: To evaluate short and long-term maternal outcome following bladder injury during cesarean delivery. STUDY DESIGN: A retrospective case series of all pregnancies complicated by full-thickness bladder injury during cesarean delivery in a single university affiliated tertiary medical center (August 2007-June 2016)...
April 5, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28400035/percutaneous-closure-of-post-myocardial-infarction-ventricular-septal-rupture-a-single-centre-experience
#17
Rajendra Kumar Premchand, Ravikanth Garipalli, T N C Padmanabhan, Geetesh Manik
BACKGROUND: Post-infarction ventricular septal rupture (VSR) is a rare but lethal mechanical complication of an acute myocardial infarction (AMI). Survival to 1 month without intervention is 6%. Given high surgical mortality, transcatheter closure has emerged as a potential strategy in selected cases. Indian data on percutaneous device closure of post AMI-VSR is scarce hence we report our single-centre experience with ASD occluder device (Amplatzer and lifetech) for closure of post-AMI VSR...
April 2017: Indian Heart Journal
https://www.readbyqxmd.com/read/28397296/reconstruction-of-late-esophagus-perforation-after-anterior-cervical-spine-fusion-with-an-adipofascial-anterolateral-thigh-free-flap-a-case-report
#18
Ruben Coelho, Tomas Ekberg, Malin Svensson, Maria Mani, Andres Rodriguez-Lorenzo
Reconstruction of late esophageal perforation usually requires flap surgery to achieve wound healing. However, restoring the continuity between the digestive tract and retropharyngeal space to allow for normal swallowing remains a technical challenge. In this report, we describe the use of a thin and pliable free adipofascial anterolateral thigh (ALT) flap in a 47-year-old tetraplegic man with a history of C5-C6 fracture presented with a large posterior esophagus wall perforation allowing an easier flap insetting for a successful wound closure...
April 11, 2017: Microsurgery
https://www.readbyqxmd.com/read/28393001/neuro-tracing-approach-to-study-kidney-innervation-a-technical-note
#19
Sanghee Lee, Anna P Malykhina
Neuro-tracing approach is a great option to study innervation of the visceral organs including the kidneys. Important factors contributing to the success of this technique include the choice of a neuro-tracer, and delivery methods to result in successful labeling of peripheral sensory and motor ganglia. The neuro-tracer is usually applied directly to the kidney accessed via a surgical opening of the abdominal wall under deep anesthesia. A series of local microinjections of the dye are performed followed by a wound closure, and recovery period from the surgery...
March 2017: Kidney Research and Clinical Practice
https://www.readbyqxmd.com/read/28386914/laparoscopy-assisted-full-thickness-resection-of-the-duodenum-for-patients-with-gastrointestinal-stromal-tumor-with-ulceration
#20
Nobutsugu Abe, Yoshikazu Hashimoto, Hirohisa Takeuchi, Atsuko Ohki, Gen Nagao, Yutaka Suzuki, Tadahiko Masaki, Toshiyuki Mori, Masanori Sugiyama
INTRODUCTION: Gastrointestinal stromal tumor (GIST) with ulceration may potentially disseminate into the peritoneal cavity after laparoscopic local wedge resection (full-thickness resection) when the intestinal wall is opened under the aeroperitoneum. To prevent this intraoperative tumor seeding, we developed laparoscopy-assisted full-thickness resection (LAFTR) of the duodenum for GIST with ulceration. Here, we present the preliminary results of LAFTR. METHODS: Three patients with duodenal GIST with ulceration underwent LAFTR...
April 7, 2017: Asian Journal of Endoscopic Surgery
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