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https://www.readbyqxmd.com/read/29764480/a-stepped-approach-for-the-management-of-symptomatic-internal-derangement-of-the-temporomandibular-joint
#1
Candan Efeoglu, Aylin Sipahi Calis, Huseyin Koca, Esra Yuksel
BACKGROUND: Internal derangement is the clinical and pathological condition of disc displacement of the temporomandibular joint. Management of these cases involve conservative and surgical treatment options. Minimally invasive surgical procedures namely arthrocentesis and arthroscopy are promising techniques in the management of internal derangement. However patient selection algorithms, indications for minimally invasive procedures and details of the techniques should be further studied for safe and cost effective management of these cases...
May 15, 2018: Journal of Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/29759498/the-extent-of-mechanical-esophageal-deviation-to-avoid-esophageal-heating-during-catheter-ablation-of-atrial-fibrillation
#2
Chandrasekar Palaniswamy, Jacob S Koruth, Alexander J Mittnacht, Marc A Miller, Subbarao Choudry, Rahul Bhardwaj, Dinesh Sharma, Jonathan M Willner, Sujata S Balulad, Elizabeth Verghese, Georgios Syros, Anurag Singh, Srinivas R Dukkipati, Vivek Y Reddy
OBJECTIVES: This study sought to determine the extent of lateral esophageal displacement required during mechanical esophageal deviation (MED) and to eliminate luminal esophageal temperature elevation (LETElev ) during pulmonary vein (PV) isolation. BACKGROUND: MED is a conceptually attractive strategy of minimizing esophageal injury while allowing uninterrupted energy delivery along the posterior left atrium during PV isolation. METHODS: MED was performed using a malleable metal stylet within a plastic tube placed within the esophagus...
October 2017: JACC. Clinical Electrophysiology
https://www.readbyqxmd.com/read/29746102/chimeric-guides-probe-and-enhance-cas9-biochemical-activity
#3
Zachary J Kartje, Christopher L Barkau, Kushal J Rohilla, Eman A Ageely, Keith T Gagnon
DNA substitutions in RNA can probe the importance of A-form struc-ture, 2'-hydroxyl contacts, and conformational constraints within RNA-guided enzymes. Using this approach, we found that Cas9 bio-chemical activity tolerated significant substitution with DNA nucleo-tides in the CRISPR RNA (crRNA). Only minimal RNA content was needed in or near the seed region. Simultaneous substitution at all positions with predicted crRNA-Cas9 2'-hydroxyl contacts had no effect on enzyme activity. The trans-activating crRNA (tracrRNA) also tolerated over 50% substitution with DNA...
May 10, 2018: Biochemistry
https://www.readbyqxmd.com/read/29745829/a-gene-profiling-deconvolution-approach-to-estimating-immune-cell-composition-from-complex-tissues
#4
Shu-Hwa Chen, Wen-Yu Kuo, Sheng-Yao Su, Wei-Chun Chung, Jen-Ming Ho, Henry Horng-Shing Lu, Chung-Yen Lin
BACKGROUND: A new emerged cancer treatment utilizes intrinsic immune surveillance mechanism that is silenced by those malicious cells. Hence, studies of tumor infiltrating lymphocyte populations (TILs) are key to the success of advanced treatments. In addition to laboratory methods such as immunohistochemistry and flow cytometry, in silico gene expression deconvolution methods are available for analyses of relative proportions of immune cell types. RESULTS: Herein, we used microarray data from the public domain to profile gene expression pattern of twenty-two immune cell types...
May 8, 2018: BMC Bioinformatics
https://www.readbyqxmd.com/read/29745639/using-alkali-metal-ions-to-template-the-synthesis-of-interlocked-molecules
#5
Alex Inthasot, Shun-Te Tung, Sheng-Hsien Chiu
In 1987, Pedersen, Cram, and Lehn were awarded the Nobel Prize in Chemistry to honor their achievements in, among other things, the selective recognition of alkali metal ions by synthetic hosts. Almost three decades later, the 2016 Nobel Prize went to Stoddart, Sauvage, and Feringa for the development of artificial molecular machines, in which interlocked molecules play a significant role. Surprisingly, although many rotaxane- and catenane-based molecular machines have been constructed using various templating approaches, alkali metal ions, which are good templates for crown ether synthesis, have only rarely been applied as templates for the assembly of these interlocked molecules...
May 10, 2018: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29728382/phylotype-level-profiling-of-lactobacilli-in-highly-complex-environments-by-means-of-an-its-based-metagenomic-approach
#6
Christian Milani, Sabrina Duranti, Marta Mangifesta, Gabriele Andrea Lugli, Francesca Turroni, Leonardo Mancabelli, Alice Viappiani, Rosaria Anzalone, Giulia Alessandri, Maria Cristina Ossiprandi, Douwe van Sinderen, Marco Ventura
The genus Lactobacillus is a widespread taxon, members of which are highly relevant to functional and fermented foods, while they are also commonly present in host-associated gut and vaginal microbiota. Substantial efforts have been undertaken to disclose the genetic repertoire of all members of the genus Lactobacillus , yet their species-level profiling in complex matrices is still undeveloped due to the poor phylotype resolution of profiling approaches based on the 16S rRNA gene. To overcome this limitation, an ITS-based profiling method was developed to accurately profile lactobacilli at species-level...
May 4, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29727002/hormone-replacement-therapy-and-opioid-tapering-for-opioid-induced-hypogonadism-among-patients-with-chronic-noncancer-pain-a-systematic-review
#7
Mahmood AminiLari, Priya Manjoo, Samantha Craigie, Rachel Couban, Li Wang, Jason W Busse
Objective: To systematically review evidence addressing the efficacy of testosterone replacement therapy (TRT) and opioid tapering for opioid-induced hypogonadism among patients with chronic noncancer pain. Study Design: Systematic review of randomized controlled trials (RCTs) and observational studies. Methods: We searched MEDLINE, CINAHL, AMED, CENTRAL, CINAHL, DARE, EMBASE, and PsycINFO through August 2017. Eligible studies enrolled ≥10 patients with chronic noncancer pain and opioid-induced hypogonadism and reported the effect of TRT or opioid tapering on a patient-important outcome collected ≥14 days after treatment...
May 2, 2018: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/29721720/comparison-of-paired-human-nasal-and-bronchial-airway-epithelial-cell-responses-to-rhinovirus-infection-and-il-13-treatment
#8
Nicole Roberts, Reem Al Mubarak, David Francisco, Monica Kraft, Hong Wei Chu
BACKGROUND: Because of its advantage as a minimally invasive procedure, nasal brushings have been increasingly used and proposed as a valuable approach to study lower airway diseases in lieu of bronchial epithelial cells. However, there is limited or conflicting evidence pertaining to whether nasal samples can be surrogates to bronchial samples. The goal of the present study is to test whether nasal epithelial cells have similar antiviral and inflammatory responses to IL-13 treatment and rhinovirus infection, a condition mimicking virally induced asthma exacerbation...
May 2, 2018: Clinical and Translational Medicine
https://www.readbyqxmd.com/read/29719907/perioperative-and-long-term-oncological-results-of-minimally-invasive-pancreatoduodenectomy-as-hybrid-technique-a-matched-pair-analysis-of-120-cases
#9
Steffen Deichmann, Louisa Romina Bolm, Kim Christin Honselmann, Ulrich Friedrich Wellner, Hryhoriy Lapshyn, Tobias Keck, Dirk Bausch
BACKGROUND: Laparoscopic pancreatoduodenectomy is a highly challenging procedure. The aim of this study was to analyse post-operative morbidity and mortality as well as long term overall survival in patients undergoing hybrid LPD, as compared to open pancreaticoduodenecomy (OPD) in a single surgeon series. METHODS: Patients undergoing pancreatoduodenectomy (PD) in the period from 2000 to 2015 were identified from a prospectively maintained database. All LPD procedures were performed by one specialised pancreatic surgeon (TK)...
April 2018: Zentralblatt Für Chirurgie
https://www.readbyqxmd.com/read/29718816/a-post-marketing-surveillance-study-of-chronic-wounds-treated-with-a-native-collagen-calcium-alginate-dressing
#10
Matthew Sabo, Lam Le, Raphael A Yaakov, Marissa Carter, Thomas E Serena
Chronic wounds (ie, wounds that fail to progress through a normal, orderly, timely sequence of repair) continue to pose significant clinical and economic burdens. A prospective, descriptive, 3-week post-marketing surveillance study was conducted across 3 wound care centers in the United States to evaluate the effectiveness of a collagen calcium alginate dressing on chronic wounds in conjunction with standard care (SC) practices (eg, offloading, debridement, compression) to support healing. Eligible participants had to be >18 years of age, have at least 1 chronic wound, and no known sensitivity to collagen...
April 2018: Ostomy/wound Management
https://www.readbyqxmd.com/read/29718662/high-spatial-resolution-imaging-of-mouse-pancreatic-islets-using-nanospray-desorption-electrospray-ionization-mass-spectrometry
#11
Ruichuan Yin, Jennifer E Kyle, Kristin E Burnum-Johnson, Kent Bloodsworth, Lori Sussel, Charles Ansong, Julia Laskin
Nanospray Desorption Electrospray Ionization mass spectrometry imaging (nano-DESI MSI) enables ambient imaging of biological samples with high sensitivity and minimal sample pretreatment. Recently, we developed an approach for constant-distance mode MSI using shear force microscopy to precisely control the distance between the sample and the nano-DESI probe. Herein, we demonstrate the power of this approach for robust imaging of pancreatic islets with high spatial resolution of ~11 μm. Pancreatic islets are difficult to characterize using traditional mass spectrometry approaches due to their small size (~100 μm) and molecular heterogeneity...
May 2, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29713986/point-counterpoint-screening-and-surveillance-for-barrett-s-esophagus-is-it-worthwhile
#12
REVIEW
Fouad Otaki, Prasad G Iyer
The exponential rise in incidence of esophageal adenocarcinoma (EAC), paired with persistently poor survival, continues to drive efforts to improve and optimize screening and surveillance practices. While advancements in endoscopic therapy have generated a shift in management and significantly improved the outcomes of patients with early-stage EAC, the majority of prevalent EAC continues to be diagnosed at advanced stages, remaining ineligible for curative therapy. Barrett's esophagus (BE) screening, when applied to high-yield target populations, using minimally or noninvasive accurate tests, followed by endoscopic surveillance to detect prevalent or incident dysplasia/EAC (which can then be treated successfully) is the cornerstone of the current BE management paradigm...
April 30, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29709359/minimally-invasive-mitral-valve-surgery-is-associated-with-excellent-resource-utilization-cost-and-outcomes
#13
Robert B Hawkins, J Hunter Mehaffey, Samuel M Kessel, Jolian J Dahl, Irving L Kron, John A Kern, Leora T Yarboro, Gorav Ailawadi
OBJECTIVES: Minimally invasive mitral valve surgery (mini-MVR) has numerous associated benefits. However, many studies fail to include greater-risk patients. We hypothesized that a minimally invasive approach in a representative cohort provides excellent outcomes with reduced resource utilization. METHODS: Mitral valve surgical records from 2011 to 2016 were paired with institutional financial records. Patients were stratified by approach and propensity-score matched to balance preoperative difference...
April 3, 2018: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29689441/development-and-validation-of-a-method-for-gefitinib-quantification-in-dried-blood-spots-using-liquid-chromatography-tandem-mass-spectrometry-application-to-finger-prick-clinical-blood-samples-of-patients-with-non-small-cell-lung-cancer
#14
Kei Irie, Saori Shobu, Seika Hiratsuji, Yuta Yamasaki, Shigeki Nanjo, Chiyuki Kokan, Akito Hata, Reiko Kaji, Katsuhiro Masago, Shiro Fujita, Yutaka Okada, Nobuyuki Katakami, Shoji Fukushima
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of gefitinib in dried blood spots (DBSs). Gefitinib was extracted with methanol from DBS of 3 mm in diameter and detected using a triple quadrupole mass spectrometer. The method was validated by evaluating its precision, accuracy, selectivity, carryover, matrix effect, recovery, and stability. For clinical validation, paired finger-prick DBS and plasma concentrations were compared for 10 patients with non-small cell lung cancer (NSCLC) taking gefitinib...
April 13, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29672399/minimally-invasive-lobectomy-is-associated-with-lower-noncancer-specific-mortality-in-elderly-patients-a-propensity-score-matched-competing-risks-analysis
#15
Boris Hristov, Takashi Eguchi, Sarina Bains, Joe Dycoco, Kay See Tan, James M Isbell, Bernard J Park, David R Jones, Prasad S Adusumilli
OBJECTIVE: To investigate cancer- and noncancer-specific mortality following lobectomy by minimally invasive surgery (MIS) versus open thoracotomy in elderly patients with nonsmall cell lung cancer (NSCLC). BACKGROUND: Two-thirds of patients with NSCLC are ≥65 years of age. As age increases, the risk of competing events, such as noncancer death, also increases. METHODS: Elderly patients (≥65 yrs of age) who have undergone curative-intent lobectomy for stage I-III NSCLC without induction therapy (2002-2013) were included (n=1,303)...
April 18, 2018: Annals of Surgery
https://www.readbyqxmd.com/read/29657895/restricted-single-isocenter-for-multiple-targets-dynamic-conformal-arc-rsimt-dca-technique-for-brain-stereotactic-radiosurgery-srs-planning
#16
Jenghwa Chang, A Gabriella Wernicke, Susan C Pannullo
Purpose/Objectives: In stereotactic radiosurgery (SRS), the multiple isocenters for multiple targets dynamic conformal arc (MIMT DCA) technique is traditionally used to treat multiple brain metastases, with one isocenter for each target. The single isocenter for multiple targets (SIMT) technique has recently been adopted to reduce the treatment time at the cost of plan quality. The objective of this study was to develop a restricted single isocenter for multiple targets DCA (RSIMT DCA) technique that can significantly reduce the treatment time but still maintain similar plan quality as the MIMT DCA technique...
2018: Journal of Radiosurgery and SBRT
https://www.readbyqxmd.com/read/29642497/computational-modelling-of-large-scale-phage-production-using-a-two-stage-batch-process
#17
Konrad Krysiak-Baltyn, Gregory J O Martin, Sally L Gras
Cost effective and scalable methods for phage production are required to meet an increasing demand for phage, as an alternative to antibiotics. Computational models can assist the optimization of such production processes. A model is developed here that can simulate the dynamics of phage population growth and production in a two-stage, self-cycling process. The model incorporates variable infection parameters as a function of bacterial growth rate and employs ordinary differential equations, allowing application to a setup with multiple reactors...
April 8, 2018: Pharmaceuticals
https://www.readbyqxmd.com/read/29628508/a-pilot-study-of-ultra-deep-targeted-sequencing-of-plasma-dna-identifies-driver-mutations-in-hepatocellular-carcinoma
#18
Ismail Labgaa, Carlos Villacorta-Martin, Delia D'Avola, Amanda J Craig, Johann von Felden, Sebastiao N Martins-Filho, Daniela Sia, Ashley Stueck, Stephen C Ward, M Isabel Fiel, Milind Mahajan, Parissa Tabrizian, Swan N Thung, Celina Ang, Scott L Friedman, Josep M Llovet, Myron Schwartz, Augusto Villanueva
Cellular components of solid tumors including DNA are released into the bloodstream, but data on circulating-free DNA (cfDNA) in hepatocellular carcinoma (HCC) are still scarce. This study aimed at analyzing mutations in cfDNA and their correlation with tissue mutations in patients with HCC. We included 8 HCC patients treated with surgical resection for whom we collected paired tissue and plasma/serum samples. We analyzed 45 specimens, including multiregional tumor tissue sampling (n = 24), peripheral blood mononuclear cells (PMBC, n = 8), plasma (n = 8) and serum (n = 5)...
April 9, 2018: Oncogene
https://www.readbyqxmd.com/read/29605184/compensation-of-signal-spillover-in-suspension-and-imaging-mass-cytometry
#19
Stéphane Chevrier, Helena L Crowell, Vito R T Zanotelli, Stefanie Engler, Mark D Robinson, Bernd Bodenmiller
The advent of mass cytometry increased the number of parameters measured at the single-cell level while decreasing the extent of crosstalk between channels relative to dye-based flow cytometry. Although reduced, spillover still exists in mass cytometry data, and minimizing its effect requires considerable expert knowledge and substantial experimental effort. Here, we describe a novel bead-based compensation workflow and R-based software that estimates and corrects for interference between channels. We performed an in-depth characterization of the spillover properties in mass cytometry, including limitations defined by the linear range of the mass cytometer and the reproducibility of the spillover over time and across machines...
March 23, 2018: Cell Systems
https://www.readbyqxmd.com/read/29587943/-a-paired-case-controlled-study-comparing-the-short-term-outcomes-of-da-vinci-rats-and-vats-approach-for-non-small-cell-lung-cancer
#20
Feng Dai, Shiguang Xu, Wei Xu, Renquan Ding, Bo Liu, Hao Meng, Yunteng Kang, Xiangrui Meng, Jie Lin, Shumin Wang
BACKGROUND: Da Vinci Surgical System is one of the greatest inventions of the 20th century, which represents the development direction of the precise minimally invasive surgical techniques, the aim of this study was to comparing the short-term outcomes between da Vinci robot-assisted lobectomy and video-assisted thoracic surgery (VATS) lobectomy for non-small cell lung cancer. METHODS: 45 pairs of non-small cell lung cancer patients underwent pulmonary lobectomy with da Vinci Robotic assisted thoracoscopic (RATS) and VATS approach during the same period from January 2014 to January 2017...
March 20, 2018: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
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