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https://www.readbyqxmd.com/read/29050333/characterization-of-cadd522-a-small-molecule-that-inhibits-runx2-dna-binding-and-exhibits-antitumor-activity
#1
Myoung Sook Kim, Ramkishore Gernapudi, Eun Yong Choi, Rena G Lapidus, Antonino Passaniti
The RUNX2 transcription factor promotes breast cancer growth and metastasis through interactions with a variety of cofactors that activate or repress target genes. Using a direct drug discovery approach we identified CADD522 as a small molecule that inhibits the DNA binding of the runt box domain protein, RUNX2. The current study defines the effect of CADD522 on breast cancer growth and metastasis, and addresses the mechanisms by which it exerts its anti-tumor activity. CADD522 treatment resulted in significant growth inhibition, clonogenic survival, tumorsphere formation, and invasion of breast cancer cells...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29049982/characterization-of-soluble-microbial-products-in-a-partial-nitrification-sequencing-batch-biofilm-reactor-treating-high-ammonia-nitrogen-wastewater
#2
Jibin Li, Jinglin Wei, Huu Hao Ngo, Wenshan Guo, Haibao Liu, Bin Du, Qin Wei, Dong Wei
In present study, the characterization of soluble microbial products (SMP) was evaluated in a partial nitrification sequencing batch biofilm reactor (SBBR). During the stable operation of SBBR, the NH4(+)-N removal efficiency and nitrite accumulation ratio were 96.70±0.41% and 93.77±1.04%, respectively. According to excitation-emission matrix (EEM), the intensities of protein-like substances were reduced under anoxic and aerobic phases, whereas humic-like substances had little change during the whole cycle...
October 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29049012/transcriptome-profiling-uncovers-potential-common-mechanisms-in-fetal-trisomies-18-and-21
#3
Marija Volk, Aleš Maver, Alenka Hodžić, Luca Lovrečić, Borut Peterlin
Human trisomies have recently been investigated using transcriptomics approaches to identify the gene expression (GE) signatures characteristic of each of these specific aneuploidy conditions. We hypothesized that the viability of cells with gross genomic imbalances might be associated with the activation of resilience mechanisms that are common to different trisomies and that are reflected by specific shared GE patterns. We report in this article our microarray GE analyses of amniocytes from fetuses with viable trisomy conditions, trisomy 21 or trisomy 18, to detect such common expression signatures...
October 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/29048645/establishment-and-characterization-of-human-osteosarcoma-cells-resistant-to-pyropheophorbide-%C3%AE-methyl-ester-mediated-photodynamic-therapy
#4
Yong Tao, Yunsheng Ou, Hang Yin, Yanyang Chen, Shenxi Zhong, Yongjian Gao, Zenghui Zhao, Bin He, Qiu Huang, Qianxing Deng
The present study was performed to establish and characterize new human osteosarcoma cell lines resistant to pyropheophorbide-α methyl ester‑mediated photodynamic therapy (MPPa-PDT). MPPa-PDT-resistant cells are isolated from the human osteosarcoma MG63 and HOS cell lines and two resistant populations were finally acquired, including MG63/PDT and HOS/PDT. Cell Counting Kit-8 (CCK-8) assay was used to determine the MPPa-PDT, cisplatin (CDDP) resistance and proliferation of MG63, MG63/PDT, HOS and HOS/PDT cells...
September 27, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29048635/novel-focal-adhesion-kinase-1-inhibitor-sensitizes-lung-cancer-cells-to-radiation-in-a-p53-independent-manner
#5
Seung-Youn Jung, Seongho Kho, Kyung-Hee Song, Jiyeon Ahn, In-Chul Park, Ky-Youb Nam, Sang-Gu Hwang, Seon-Young Nam, Seong-Jun Cho, Jie-Young Song
Focal adhesion kinase 1 (FAK1) is known to promote tumor progression and metastasis by controlling cell movement, invasion, survival and the epithelial-to-mesenchymal transition in the tumor microenvironment. As recent reports imply that FAK1 is highly associated with tumor cell development and malignancy, the inhibition of FAK1 activity could be an effective therapeutic approach for inhibiting the growth and metastasis of tumor cells. In this study, we aimed to determine the effect of a novel synthetic FAK1 inhibitor 2-[2-(2-methoxy-4-morpholin-4-yl-phenylamino)-5-trifluoromethyl-pyrimidin-4-ylamino]-N-methyl-benzamide, (MPAP) on lung cancer cells...
September 29, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29048622/enhancement-of-cytotoxic-effects-of-gemcitabine-by-dclk1-inhibition-through-suppression-of-chk1-phosphorylation-in-human-pancreatic-cancer-cells
#6
Daichi Kawamura, Yoshihiro Takemoto, Arata Nishimoto, Koji Ueno, Tohru Hosoyama, Bungo Shirasawa, Toshiki Tanaka, Naruji Kugimiya, Eijiro Harada, Kimikazu Hamano
Although gemcitabine (GEM) is frequently used in the treatment of pancreatic cancer, the effects are limited. To increase the inhibitory effect of GEM, the identification of a molecular target is needed. Recent studies have revealed that doublecortin-like kinase 1 (Dclk1) positively regulates tumor growth, invasion, metastasis, factors related to epithelial-mesenchymal transition (EMT), pluripotency, angiogenesis, and anti-apoptosis in pancreatic cancer cells. Therefore, Dclk1 is a potential therapeutic target for pancreatic cancer...
September 20, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29048444/substoichiometric-ribose-methylations-in-spliceosomal-snrnas
#7
Nicolai Krogh, Martin Kongsbak-Wismann, Carsten Geisler, Henrik Nielsen
Sequencing-based profiling of ribose methylations is a new approach that allows for experiments addressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells...
October 19, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29048351/bacterial-substrate-transformation-tracked-by-stable-isotope-guided-nmr-metabolomics-application-in-a-natural-aquatic-microbial-community
#8
Mario Uchimiya, Yuuri Tsuboi, Kengo Ito, Yasuhiro Date, Jun Kikuchi
The transformation of organic substrates by heterotrophic bacteria in aquatic environments constitutes one of the key processes in global material cycles. The development of procedures that would enable us to track the wide range of organic compounds transformed by aquatic bacteria would greatly improve our understanding of material cycles. In this study, we examined the applicability of nuclear magnetic resonance spectroscopy coupled with stable-isotope labeling to the investigation of metabolite transformation in a natural aquatic bacterial community...
October 19, 2017: Metabolites
https://www.readbyqxmd.com/read/29047357/effects-of-inbreeding-and-other-systematic-effects-on-fertility-of-black-forest-draught-horses-in-germany
#9
Maarit Müller-Unterberg, Sandra Wallmann, Ottmar Distl
BACKGROUND: The Black Forest Draught horse (BFDH) is an endangered German coldblood breed with its origin in the area of the Black Forest in South Germany. In this retrospective study, the influence of the inbreeding coefficient on foaling rates was investigated using records from ten breeding seasons. Due to the small population size of BFDH, the level of inbreeding is increasing and may have an effect on foaling rates.The data of the present study included all coverings reported for 1024 BFDH mares in the years 2001-2009...
October 18, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/29047294/deep-transverse-metatarsal-ligament-transection-in-morton-s-neuroma-excision-a-cadaveric-study-examining-effects-on-metatarsal-alignment
#10
Nathaniel Preston, Daniel Peterson, Jamey Allen, Jill S Kawalec, Jeffrey Whitaker
BACKGROUND: In the dorsal incisional approach for Morton's neuroma, it is required to transect the deep transverse metatarsal ligament (DTML) that lies in the interspace between the third and fourth metatarsal heads. The purpose of this study was to evaluate the relationship between transection of the DTML in the third intermetatarsal space and the metatarsal alignment. METHODS: Nine human cadaveric lower extremity limbs were used for this study. Each limb was mounted to the MTS 858 Mini Bionix biomechanical test system and loaded to 120% of the donor's documented body weight at a rate of 15 lbf/s, in order to simulate peak weightbearing ground reactive forces on the forefoot...
October 1, 2017: Foot & Ankle Specialist
https://www.readbyqxmd.com/read/29046991/implications-of-allometric-model-selection-for-county-level-biomass-mapping
#11
Laura Duncanson, Wenli Huang, Kristofer Johnson, Anu Swatantran, Ronald E McRoberts, Ralph Dubayah
BACKGROUND: Carbon accounting in forests remains a large area of uncertainty in the global carbon cycle. Forest aboveground biomass is therefore an attribute of great interest for the forest management community, but the accuracy of aboveground biomass maps depends on the accuracy of the underlying field estimates used to calibrate models. These field estimates depend on the application of allometric models, which often have unknown and unreported uncertainties outside of the size class or environment in which they were developed...
October 18, 2017: Carbon Balance and Management
https://www.readbyqxmd.com/read/29046620/the-dental-calculus-metabolome-in-modern-and-historic-samples
#12
Irina M Velsko, Katherine A Overmyer, Camilla Speller, Lauren Klaus, Matthew J Collins, Louise Loe, Laurent A F Frantz, Krithivasan Sankaranarayanan, Cecil M Lewis, Juan Bautista Rodriguez Martinez, Eros Chaves, Joshua J Coon, Greger Larson, Christina Warinner
INTRODUCTION: Dental calculus is a mineralized microbial dental plaque biofilm that forms throughout life by precipitation of salivary calcium salts. Successive cycles of dental plaque growth and calcification make it an unusually well-preserved, long-term record of host-microbial interaction in the archaeological record. Recent studies have confirmed the survival of authentic ancient DNA and proteins within historic and prehistoric dental calculus, making it a promising substrate for investigating oral microbiome evolution via direct measurement and comparison of modern and ancient specimens...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/29046452/deep-sequencing-of-foot-and-mouth-disease-virus-reveals-rna-sequences-involved-in-genome-packaging
#13
Grace Logan, Joseph Newman, Caroline F Wright, Lidia Lasecka-Dykes, Daniel T Haydon, Eleanor M Cottam, Tobias J Tuthill
Non-enveloped viruses protect their genomes by packaging them into an outer shell or capsid of virus-encoded proteins. Packaging and capsid assembly in RNA viruses can involve interactions between capsid proteins and secondary structures in the viral genome as exemplified by the RNA bacteriophage MS2 and as proposed for other RNA viruses of plants, animals and human. In the picornavirus family of non-enveloped RNA viruses, the requirements for genome packaging remain poorly understood. Here we show a novel and simple approach to identify predicted RNA secondary structures involved in genome packaging in the picornavirus foot-and-mouth disease virus (FMDV)...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29046388/the-early-dendritic-cell-signaling-induced-by-virulent-francisellatularensis-strain-occurs-in-phases-and-involves-the-activation-of-erks-and-p38-in-the-later-stage
#14
Ivo Fabrik, Marek Link, Daniela Putzova, Lenka Plzakova, Zuzana Lubovska, Vlada Philimonenko, Ivona Pavkova, Pavel Rehulka, Zuzana Krocova, Pavel Hozak, Marina Santic, Jiri Stulik
Dendritic cells (DCs) infected by Francisella tularensis are poorly activated and do not undergo classical maturation process. While reasons of such unresponsiveness are not fully understood, their impact on the priming of immunity is well appreciated. Previous attempts to explain the behavior of Francisella-infected DCs were hypothesis-driven and focused on events at later stages of infection. Here, we took an alternative unbiased approach by applying methods of global phosphoproteomics to analyze the dynamics of cell signaling in primary DCs during the first hour of infection by Francisella tularensis...
October 18, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29046028/-aria-2016-executive-summary-integrated-care-pathways-for-predictive-medicine-throughout-the-life-cycle-in-argentina
#15
Juan Carlos Ivancevich, Hugo Neffen, Mario E Zernotti, Estrella Asayag, Ariel Blua, Alberto Cicerán, Edgardo J Jares, Alberto J Lavrut, Jorge F Máspero, Ioana Agache, Claus Bachert, Anna Bedbrook, Giorgio W Canonica, Thomas B Casale, Álvaro A Cruz, Wytske J Fokkens, Peter W Hellings, Boleslaw Samolinski, Jean Bousquet
The ARIA initiative was started during a World Health Organization workshop in 1999. The initial goals were to propose a new classification for allergic rhinitis, to promote the concept of multi-morbidity in asthma and rhinitis and to develop guidelines with stakeholders for world-wide use. ARIA is now focused on the implementation of emerging technologies for individualized and predictive medicine. MASK: MACVIA-Aria Sentinel Network uses mobile technology to develop care pathways that enable management by a multidisciplinary group or by patients themselves...
July 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/29045061/suppression-of-mapk-signaling-in-braf-activated-pten-deficient-melanoma-by-blocking-%C3%AE-catenin-signaling-in-cancer-associated-fibroblasts
#16
Linli Zhou, Kun Yang, Spencer Dunaway, Zalfa Abdel-Malek, Thomas Andl, Ana Luisa Kadekaro, Yuhang Zhang
Cancer-associated fibroblasts (CAFs) in the tumor microenvironment have been associated with formation of a dynamic and optimized niche for tumor cells to grow and evade cell death induced by therapeutic agents. We recently reported that ablation of β-catenin expression in stromal fibroblasts and CAFs disrupted their biological activities in in vitro studies and in an in vivo B16F10 mouse melanoma model. Here, we show that the development of a BRAF-activated PTEN-deficient mouse melanoma was significantly suppressed in vivo after blocking β-catenin signaling in CAFs...
October 17, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/29044433/pregnancy-induced-changes-in-metabolome-and-proteome-in-ovine-uterine-flushings
#17
Jared J Romero, Bethany E Liebig, Corey D Broeckling, Jessica E Prenni, Thomas R Hansen
Mass spectrometry (MS) approaches were used herein to identify metabolites and proteins in uterine flushings (UF) that may contribute to nourishing the conceptus. Ovine uteri collected on Day 12 of the estrous cycle (n = 5 ewes exposed to vasectomized ram) or Days 12 (n = 4), 14 (n = 5), or 16 (n = 5) of pregnancy (bred with fertile ram) were flushed using buffered saline. Metabolites were extracted using 80% methanol and profiled using ultraperformance liquid chromatography (LC) tandem mass spectrometry. The proteome was examined by digestion with trypsin, followed by the analysis of peptides with LC-MS/MS...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29044370/the-ecology-and-epidemiology-of-ross-river-and-murray-valley-encephalitis-viruses-in-western-australia-examples-of-one-health-in-action
#18
John S Mackenzie, Michael D A Lindsay, David W Smith, Allison Imrie
Arboviruses are maintained and transmitted through an alternating biological cycle in arthropods and vertebrates, with largely incidental disease in humans and animals. As such, they provide excellent examples of One Health, as their health impact is inextricably linked to their vertebrate hosts, their arthropod vectors and the environment. Prevention and control requires a comprehensive understanding of these interactions, and how they may be effectively and safely modified. This review concentrates on human disease due to Ross River and Murray Valley encephalitis viruses, the two major arboviral pathogens in Australia...
June 1, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29043797/metal-oxides-in-surface-sediment-control-nickel-bioavailability-to-benthic-macroinvertebrates
#19
Raissa Marques Mendonca, Jennifer M Daley, Michelle L Hudson, Christian Schlekat, G Allen Burton, David Costello
In aquatic ecosystems, the cycling and toxicity of nickel (Ni) are coupled to other elemental cycles that can limit its bioavailability. Current sediment risk assessment approaches consider acid-volatile sulfide (AVS) as the major binding phase for Ni, but have not yet incorporated ligands that are present in oxic sediments. Our study aimed to assess how metal oxides play a role in Ni bioavailability in surficial sediments exposed to effluent from two mine sites. We coupled spatially explicit sediment geochemistry (i...
October 18, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29043637/assays-to-study-repair-of-inducible-dna-double-strand-breaks-at-telomeres
#20
Roxanne Oshidari, Karim Mekhail
The ends of linear chromosomes are constituted of repetitive DNA sequences called telomeres. Telomeres, nearby regions called subtelomeres, and their associated factors prevent chromosome erosion over cycles of DNA replication and prevent chromosome ends from being recognized as DNA double-strand breaks (DSBs). This raises the question of how cells repair DSBs that actually occur near chromosome ends. One approach is to edit the genome and engineer cells harboring inducible DSB sites within the subtelomeric region of different chromosome ends...
2018: Methods in Molecular Biology
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