Ai-hua Liao, Rebmann Vera, Grosse-Wilde Hans, Yan Wang, Ling Zhang
AIM: To explore the optimal in vitro culture condition for producing antibodies to HLA class I molecules by human B lymphocyte cells. METHODS: By applying EBV-transformed B lymphoblast cell line (BLCL) from a sensitized kidney transplanted subject as a model, the capacity of Ig and specific HLA class I antibody production of BLCL cells was investigated by ELISA and ELISpot, under three different culture systems, RPMI1640+OPTI mAb, RPMI1640+0.1 mmol/L non-essential amino acid+1 mmol/L sodium pyruvate+40 mg/L apo-transferrin,and serum free medium (SFM) for hybridoma culture, and three different culture conditions, culture medium only, culture medium supplemented with IL-4, IL-10 and alpha-CD40, and culture medium supplemented with IL-4, IL-10 and CD40L...
July 2007: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology