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lactobacillus rhamnosus

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https://www.readbyqxmd.com/read/28230607/effects-of-probiotics-on-nonalcoholic-fatty-liver-disease-in-obese-children-and-adolescents
#1
Fatemeh Famouri, Zainab Shariat, Mahin Hashemipour, Mojtaba Keikha, Roya Kelishadi
OBJECTIVES: This study aims to evaluate the effects of some probiotics on sonographic and biochemical nonalcoholic fatty liver disease (NAFLD). METHODS: This randomized triple-blind trial was conducted among 64 obese children with sonographic NAFLD. They were randomly allocated to receive probiotic capsule (containing Lactobacillus acidophilus ATCC B3208, 3 × 10 colony forming units [CFU]; Bifidobacterium lactis DSMZ 32269, 6 × 10 CFU; Bifidobacterium bifidum ATCC SD6576, 2 × 10 CFU; Lactobacillus rhamnosus DSMZ 21690, 2 × 10 CFU) or placebo for 12 weeks...
March 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28213763/the-lactobacillus-rhamnosus-and-lactobacillus-fermentum-strains-from-human-biotopes-characterized-with-mlst-and-toxin-antitoxin-gene-polymorphism
#2
E U Poluektova, R A Yunes, M V Epiphanova, V S Orlova, V N Danilenko
The diversity of Lb. rhamnosus and Lb. fermentum strains isolated from feces, saliva, and the vaginal cavity of 18-22-year-old healthy women residing in central regions of the Russian Federation has been characterized. The results obtained using multilocus sequence typing were identical to those obtained with the analysis of genetic and genomic polymorphism in TA systems. Different as well as identical Lb. rhamnosus and Lb. fermentum sequence types (ST) were isolated from various parts of the body of the same person...
February 17, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28210256/transcriptomic-analysis-of-the-innate-antiviral-immune-response-in-porcine-intestinal-epithelial-cells-influence-of-immunobiotic-lactobacilli
#3
Leonardo Albarracin, Hisakazu Kobayashi, Hikaru Iida, Nana Sato, Tomonori Nochi, Hisashi Aso, Susana Salva, Susana Alvarez, Haruki Kitazawa, Julio Villena
Lactobacillus rhamnosus CRL1505 and Lactobacillus plantarum CRL1506 are immunobiotic strains able to increase protection against viral intestinal infections as demonstrated in animal models and humans. To gain insight into the host-immunobiotic interaction, the transcriptomic response of porcine intestinal epithelial (PIE) cells to the challenge with viral molecular associated pattern poly(I:C) and the changes in the transcriptomic profile induced by the immunobiotics strains CRL1505 and CRL1506 were investigated in this work...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28193570/live-and-heat-killed-lactobacillus-spp-interfere-with-streptococcus-mutans-and-streptococcus-oralis-during-biofilm-development-on-titanium-surface
#4
E Ciandrini, R Campana, W Baffone
OBJECTIVES: This research investigates the ability of live and heat-killed (HK) Lactic Acid Bacteria (LAB) to interfere with Streptococcus mutans ATCC 25175 and Streptococcus oralis ATCC 9811 during biofilm formation. DESIGN: Eight Lactobacillus spp. and two oral colonizers, pathogenic Streptococcus mutans and resident Streptococcus oralis, were characterized for their aggregation abilities, cell surface properties and biofilm formation ability on titanium surface...
February 4, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28193434/the-effect-of-non-standard-heat-treatment-of-sheep-s-milk-on-physico-chemical-properties-sensory-characteristics-and-the-bacterial-viability-of-classical-and-probiotic-yogurt
#5
Šimun Zamberlin, Dubravka Samaržija
Classical and probiotic set yogurt were made using non-standard heat treatment of sheep's milk at 60°C/5min. Physico-chemical properties, sensory characteristics, and the viability of bacteria that originated from cultures in classical and probiotic yogurt were analysed during 21days of storage at 4°C. For the production of yogurt, a standard yogurt culture and a probiotic strain Lactobacillus rhamnosus GG were used. At the end of storage time of the classical and probiotic yogurt the totals of non-denatured whey proteins were 92...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28185076/prebiotic-effects-of-bovine-lactoferrin-on-specific-probiotic-bacteria
#6
Po-Wen Chen, Zhen-Shu Liu, Tai-Chen Kuo, Min-Chi Hsieh, Zhe-Wei Li
Bovine lactoferrin (bLf) is a natural iron-binding protein and it has been suggested to be a prebiotic agent, but this finding remains inconclusive. This study explores the prebiotic potential of bLf in 14 probiotics. Initially, bLf (1-32 mg/mL) treatment showed occasional and slight prebiotic activity in several probiotics only during the late experimental period (48, 78 h) at 37 °C. We subsequently supposed that bLf exerts stronger prebiotic effects when probiotic growth has been temperately retarded...
February 9, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28178767/probiotic-bacteria-inhibit-the-bovine-respiratory-pathogen-mannheimia-haemolytica-serotype-1-in-vitro
#7
S Amat, S Subramanian, E Timsit, T W Alexander
This study evaluated the potential of probiotic bacteria to inhibit growth and cell adhesion of the bovine respiratory pathogen Mannheimia haemoltyica serotype 1. The inhibitory effects of nine probiotic strains (Lactobacillus acidophilus, L. casei, L. helveticus, L. plantarum, L. rhamnosus, Lactococcus lactis, Streptococcus thermophilus and two Paenibacillus polymyxa strains) against M. haemolytica were evaluated using a spot-on-lawn method. Probiotic strains were then tested for their adherence to bovine bronchial epithelial (BBE) cells and the ability to displace and compete against M...
February 8, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28165193/polysaccharides-l900-2-and-l900-3-isolated-from-lactobacillus-rhamnosus-lock-0900-modulate-allergic-sensitization-to-ovalbumin-in-a-mouse-model
#8
Sabina Górska, Martin Schwarzer, Dagmar Srutkova, Petra Hermanova, Ewa Brzozowska, Hana Kozakova, Andrzej Gamian
Here, we compared the abilities of polysaccharides L900/2 and L900/3, which were previously isolated from Lactobacillus rhamnosus LOCK 0900, to modulate the immune response to bystander antigens in a mouse model of ovalbumin (OVA) sensitization. In vivo, both polysaccharides reduced the levels of OVA-specific IgE, IgE-dependent basophil degranulation and IgG2a antibodies, but had no effect on the levels of OVA-specific IgA or IgG1. Interestingly, both polysaccharides triggered recall cellular responses with distinct properties...
February 6, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28161181/effects-of-bacterial-direct-fed-microbials-on-ruminal-characteristics-methane-emission-and-milk-fatty-acid-composition-in-cows-fed-high-or-low-starch-diets
#9
C Philippeau, A Lettat, C Martin, M Silberberg, D P Morgavi, A Ferlay, C Berger, P Nozière
This study investigated the effects of bacterial direct-fed microbials (DFM) on ruminal fermentation and microbial characteristics, methane (CH4) emission, diet digestibility, and milk fatty acid (FA) composition in dairy cows fed diets formulated to induce different ruminal volatile fatty acid (VFA) profiles. Eight ruminally cannulated dairy cows were divided into 2 groups based on parity, days in milk, milk production, and body weight. Cows in each group were fed either a high-starch (38%, HS) or a low-starch (2%, LS) diet in a 55:45 forage-to-concentrate ratio on a dry matter (DM) basis...
February 1, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28160762/dietary-therapy-in-patients-with-inflammatory-arthritis
#10
Rauli Mäkelä, Heli Mäkilä, Ritva Peltomaa
Context • The exact etiology of rheumatoid disease is currently unknown. Changes in the microbiota of the gastrointestinal (GI) tract, chronic bacterial infection of the upper urinary tract, increased permeability of the GI tract, and food sensitivities have been classified among the factors that may cause or aggravate the disease. Dietary deficiencies also may render patients more susceptible to inflammatory conditions and disorders of the immunological system. Objective • The study intended to determine the effects of a therapy consisting of dietary elimination, dietary supplements, and probiotics, collectively called DDP therapy, which is given either independently or in combination with conventional pharmacological agents...
January 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/28132503/effect-of-drying-methods-on-protein-and-dna-conformation-changes-in-lactobacillus-rhamnosus-gg-cells-by-fourier-transform-infrared-spectroscopy
#11
Mya M Hlaing, Bayden R Wood, Don McNaughton, DanYang Ying, Geoff Dumsday, Mary Ann Augustin
Microencapsulation protects cells against environmental stress encountered during the production of probiotics, which are used as live microbial food ingredients. Freeze-drying and spray-drying are used in the preparation of powdered microencapsulated probiotics. This study examines the ability of Fourier transform infrared (FTIR) spectroscopy to detect differences in cells exposed to freeze-drying and spray-drying of encapsulated Lactobacillus rhamnosus GG cells. The FTIR analysis clearly demonstrated there were more significant molecular changes in lipid, fatty acid content, protein, and DNA conformation of nonencapsulated compared to encapsulated bacterial cells...
February 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28109094/-lactobacillus-rhamnosus-gg-conditioned-medium-prevents-e-coli-meningitis-by-inhibiting-nuclear-factor-%C3%AE%C2%BAb-pathway
#12
Qing Zeng, Xiao-Long He, Han-Sheng Xiao, Lei DU, Yu-Jing Li, Le-Cheng Chen, Hui-Wen Tian, Sheng-He Huang, Hong Cao
OBJECTIVE: To investigate whether Lactobacillus rhamnosus GG conditioned medium(LGG-CM)has preventive effect against E. coli K1-induced neuropathogenicity in vitro by inhibiting nuclear factor-κB (NF-κB) signaling pathway. METHODS: An in vitro blood-brain barrier (BBB) model was constructed using human brain microvascular endothelial cells (HBMECs). The effect of LGG-CM on E. coli-actived NF-κB signaling pathway was assayed using Western blotting. Invasion assay and polymorphonuclear leukocyte (PMN) transmigration assay were performed to explore whether LGG-CM could inhibit E...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28107866/potential-of-zimbabwean-commercial-probiotic-products-and-strains-of-lactobacillus-plantarum-as-prophylaxis-and-therapy-against-diarrhoea-caused-by-escherichia-coli-in-children
#13
Walter Chingwaru, Jerneja Vidmar
OBJECTIVE: To evaluate the potential of commercial fermented products sold in the country, and strains of Lactobacillus plantarum (L. plantarum) as prophylaxis and therapy against diarrhoea in children. METHODS: The antimicrobial potential of cultures of lactobacilli enriched from 4 Zimbabwean commercial food/beverage products: Dairibord Lacto sour milk (DLSM), Probrand sour milk (PSM), Kefalos Vuka cheese (KVC) and Chibuku opaque beer (COB); and four strains of L...
January 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28106205/synbiotic-amazonian-palm-berry-a%C3%A3-ai-euterpe-oleracea-mart-ice-cream-improved-lactobacillus-rhamnosus-gg-survival-to-simulated-gastrointestinal-stress
#14
Mayra Garcia Maia Costa, Gabriela Namur Ooki, Antônio Diogo Silva Vieira, Raquel Bedani, Susana Marta Isay Saad
The effect of açai pulp ice cream and of its supplementation with inulin (I), whey protein concentrate (WC), and/or whey protein isolate (WI) on the viability and resistance to simulated gastrointestinal stress of the probiotic Lactobacillus (Lb.) rhamnosus GG strain throughout storage at -18 °C for up to 112 days was evaluated and morphological changes during stress were monitored. Lb. rhamnosus GG viability was stable in all formulations for up to 112 days of storage, preserving populations around 9 log CFU g(-1)...
January 20, 2017: Food & Function
https://www.readbyqxmd.com/read/28103868/probiotics-for-vaginal-health-in-south-africa-what-is-on-retailers-shelves
#15
Anna-Ursula Happel, Shameem Z Jaumdally, Tanya Pidwell, Tracy Cornelius, Heather B Jaspan, Remy Froissart, Shaun L Barnabas, Jo-Ann S Passmore
BACKGROUND: Probiotics are widely used to improve gastrointestinal (GI) health, but they may also be useful to prevent or treat gynaecological disorders, including bacterial vaginosis (BV) and candidiasis. BV prevalence is high in South Africa and is associated with increased HIV risk and pregnancy complications. We aimed to assess the availability of probiotics for vaginal health in retail stores (pharmacies, supermarkets and health stores) in two major cities in South Africa. METHODS: A two-stage cluster sampling strategy was used in the Durban and Cape Town metropoles...
January 19, 2017: BMC Women's Health
https://www.readbyqxmd.com/read/28100014/mg-2-improves-the-thermotolerance-of-probiotic-lactobacillus-rhamnosus-gg-lactobacillus-casei-zhang-and-lactobacillus-plantarum-p-8
#16
Yi Yang, Song Huang, Juan Wang, Gwénaël Jan, Romain Jeantet, Xiao Dong Chen
Food related carbohydrates and proteins are often used as thermoprotectant for probiotic lactobacilli during industrial production and processing. However, the effect of inorganic salts is rarely reported. Magnesium is the second-most abundant cation in bacteria, and commonly found in various foods. Mg(2+) homeostasis is important in Salmonella and has been reported to play a critical role in their thermotolerance. However, Mg(2+) role in thermotolerance of other bacteria, in particular probiotic bacteria, still remains a hypothesis...
January 18, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28079729/effect-of-probiotics-on-diarrhea-in-children-with-severe-acute-malnutrition-a-randomized-controlled-study-in-uganda
#17
Benedikte Grenov, Hanifa Namusoke, Betty Lanyero, Nicolette Nabukeera-Barungi, Christian Ritz, Christian Mølgaard, Henrik Friis, Kim F Michaelsen
OBJECTIVES: To assess the effect of probiotics on diarrhea during in- and outpatient treatment of children with severe acute malnutrition (SAM). METHODS: A randomized, double-blind, placebo-controlled study was conducted involving 400 children admitted with SAM. Patients received one daily dose of a blend of Bifidobacterium animalis subsp. lactis (BB-12) and Lactobacillus rhamnosus (LGG) (10 billion colony-forming units, 50:50) or placebo during hospitalization followed by an 8-12 week outpatient treatment period, depending on patients' recovery rate...
January 11, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28073366/oral-treatment-with-lactobacillus-rhamnosus-attenuates-behavioural-deficits-and-immune-changes-in-chronic-social-stress
#18
Aadil Bharwani, M Firoz Mian, Michael G Surette, John Bienenstock, Paul Forsythe
BACKGROUND: Stress-related disorders involve systemic alterations, including disruption of the intestinal microbial community. Given the putative connections between the microbiota, immunity, neural function, and behaviour, we investigated the potential for microbe-induced gut-to-brain signalling to modulate the impact of stress on host behaviour and immunoregulation. METHODS: Male C57BL/6 mice treated orally over 28 days with either Lactobacillus rhamnosus (JB-1) ™ or vehicle were subjected to chronic social defeat and assessed for alterations in behaviour and immune cell phenotype...
January 11, 2017: BMC Medicine
https://www.readbyqxmd.com/read/28069821/characterization-of-engineered-preq1-riboswitches-for-inducible-gene-regulation-in-mycobacteria
#19
Erik R Van Vlack, Shana Topp, Jessica C Seeliger
: We report here the behavior of naturally occurring and rationally engineered preQ1 riboswitches and their application to inducible gene regulation in mycobacteria. Because mycobacteria lack preQ1 biosynthetic genes, we hypothesized that preQ1 could be used as an exogenous non-metabolite ligand to control riboswitches in mycobacteria. Selected naturally occurring preQ1 riboswitches were assayed and successfully drove preQ1-dependent repression of a GFP reporter in Mycobacterium smegmatis Using structure-based design, we engineered three preQ1 riboswitches from Thermoanaerobacter tencongensis, Bacillus subtilis, and Lactobacillus rhamnosus towards achieving higher response ratios and increased repression...
January 9, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28067041/utility-of-thermostable-xylanases-of-mycothermus-thermophilus-in-generating-prebiotic-xylooligosaccharides
#20
Rui Ma, Yingguo Bai, Huoqing Huang, Huiying Luo, Sanfeng Chen, Yunliu Fan, Lei Cai, Bin Yao
Xylooligosaccharides as emerging prebiotics are able to promote the growth of probiotic bacteria. In the present study, four neutral, thermostable xylanases (MtXyn11A, MtXyn11At, MtXyn11B, and MtXyn11C) from compost fungus Mycothermus thermophilus CGMCC3.18119 were overexpressed in Pichia pastoris GS115 and used to produce xylooligosaccharides from beechwood xylan. The enzymes showed similar enzymatic properties (maximal activities at pH 6.0-6.5 and 65 °C) but varied in catalytic efficiency and cleaving actions...
February 3, 2017: Journal of Agricultural and Food Chemistry
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