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lactobacillus rhamnosus

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https://www.readbyqxmd.com/read/29236340/flow-cytometry-a-versatile-technology-for-specific-quantification-and-viability-assessment-of-microorganisms-in-multi-strain-probiotic-products
#1
Camille Chiron, Thomas A Tompkins, Pierre Burguière
AIMS: Classical microbiology techniques are the gold standard for probiotic enumeration. However, these techniques are limited by parameters of time, specificity and incapacity to detect viable but non-culturable (VBNC) microorganisms and non-viable cells. The aim of the study was to evaluate flow cytometry as a novel method for the specific quantification of viable and non-viable probiotics in multi-strain products. METHODS AND RESULTS: Custom polyclonal antibodies were produced against five probiotic strains from different species (Bifidobacterium bifidum R0071, Bifidobacterium longum ssp...
December 13, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29231922/gene-expression-profiling-in-the-intestinal-mucosa-of-obese-rats-administered-probiotic-bacteria
#2
Julio Plaza-Díaz, Cándido Robles-Sánchez, Francisco Abadía-Molina, María José Sáez-Lara, Laura María Vilchez-Padial, Ángel Gil, Carolina Gómez-Llorente, Luis Fontana
We investigated whether the administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 modulate the expression of genes in the intestinal mucosa of obese Zucker rats. Forty-eight Zucker-Leprfa/fa and 16 Zucker lean Lepr+/fa rats were used. Eight Zucker lean Lepr+/fa and 8 Zucker-Leprfa/fa rats were euthanized as a reference. The remaining 40 Zucker-Leprfa/fa rats were then assigned to receive 1010 colony forming units (CFU) of one of the three probiotic strains, a mixture of L...
December 12, 2017: Scientific Data
https://www.readbyqxmd.com/read/29220366/the-distinct-effects-of-orally-administered-lactobacillus-rhamnosus-gg-and-lactococcus-lactis-subsp-lactis-c59-on-gene-expression-in-the-murine-small-intestine
#3
Chise Suzuki, Ayako Aoki-Yoshida, Reiji Aoki, Keisuke Sasaki, Yoshiharu Takayama, Koko Mizumachi
The molecular mechanisms of strain-specific probiotic effects and the impact of the oral administration of probiotic strains on the host's gene expression are not yet well understood. The aim of this study was to investigate the strain-specific effects of probiotic strain intake on gene expression in the murine small intestine. Two distinct strains of lactic acid bacteria, Lactobacillus rhamnosus GG (GG) and Lactococcus lactis subsp. lactis C59 (C59), were orally administered to BALB/c mice, daily for 2 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/29218505/antibacterial-and-antibiofilm-activity-of-lactic-acid-bacteria-isolated-from-traditional-artisanal-milk-cheese-from-northeast-china-against-enteropathogenic-bacteria
#4
Xingyang Cui, Yunjia Shi, Shanshan Gu, Xin Yan, Hongyan Chen, Junwei Ge
The present study aims to investigate the probiotic properties of novel strains of lactic acid bacteria isolated from traditional artisanal milk cheese from Northeast China and to explore their antibacterial activity against enteropathogenic bacteria. Of the 321 isolates, 86 exhibited survival in low pH, resistance to pancreatin, and tolerance to bile salts; of these, 12 inhibited the growth of more than seven enteropathogenic bacteria and exhibited antibiofilm activities against Staphylococcus aureus CMCC26003 and/or Escherichia coli CVCC230...
December 7, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29212822/effect-of-probiotics-on-pharmacokinetics-of-orally-administered-acetaminophen-in-mice
#5
Jeon-Kyung Kim, Min Sun Choi, Jin-Ju Jeong, Su-Min Lim, In Sook Kim, Hye Hyun Yoo, Dong-Hyun Kim
Orally administered probiotics change gut microbiota composition and enzyme activities. Thus, coadministration of probiotics with drugs may lead to changes in the pharmacokinetic parameters of the drugs. In this study, we investigated the pharmacokinetics of acetaminophen in rats treated with probiotics. Oral administration of probiotics changed the gut microbiota composition in the mice. Of these probiotics, Lactobacillus reuteri K8 increased the numbers of clostridia, bifidobacteria, and enterococci, and Lactobacillus rhamnosus K9 decreased the number of bifidobacteria, determined by culturing in selective media...
December 6, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29204452/recurrent-lactobacillus-bacteremia-in-a-patient-with-leukemia
#6
Paurush Ambesh, Sarah Stroud, Eva Franzova, Joseph Gotesman, Kavita Sharma, Lawrence Wolf, Stephan Kamholz
Lactobacillus species are a commensal flora of the human gastrointestinal and the female genitourinary tract. Lactobacilli especially the rhamnosus species, are common components of commercial probiotics. They are rarely associated with pathology in immunocompetent people, but they have been known to cause dental caries, bacteremia, and endocarditis in patients with suppressed immune function. Cases of Lactobacillus bacteremia have been reported in patients with acute myeloid leukemia, large granular lymphocytic leukemia, and in transplant recipients...
October 2017: Journal of Investigative Medicine High Impact Case Reports
https://www.readbyqxmd.com/read/29198543/exposure-to-gastric-acid-inhibitors-increases-the-risk-of-infection-in-preterm-very-low-birth-weight-infants-but-concomitant-administration-of-lactoferrin-counteracts-this-effect
#7
Paolo Manzoni, Ruben García Sánchez, Michael Meyer, Ilaria Stolfi, Lorenza Pugni, Hubert Messner, Silvia Cattani, Pasqua Maria Betta, Luigi Memo, Lidia Decembrino, Lina Bollani, Matteo Rinaldi, Maria Fioretti, Michele Quercia, Milena Maule, Elena Tavella, Alessandro Mussa, Chryssoula Tzialla, Nicola Laforgia, Fabio Mosca, Rosario Magaldi, Michael Mostert, Daniele Farina
OBJECTIVE: To investigate whether exposure to inhibitors of gastric acidity, such as H2 blockers or proton pump inhibitors, can independently increase the risk of infections in very low birth weight (VLBW) preterm infants in the neonatal intensive care unit. STUDY DESIGN: This is a secondary analysis of prospectively collected data from a multicenter, randomized controlled trial of bovine lactoferrin (BLF) supplementation (with or without the probiotic Lactobacillus rhamnosus GG) vs placebo in prevention of late-onset sepsis (LOS) and necrotizing enterocolitis (NEC) in preterm infants...
November 30, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/29195991/molecular-identification-and-technological-characterization-of-lactic-acid-bacteria-isolated-from-fermented-kidney-beans-flours-phaseolus-vulgaris-l-and-p-coccineus-in-northwestern-argentina
#8
Gabriel D Sáez, Elvira M Hébert, Lucila Saavedra, Gabriela Zárate
Legumes are an important protein source in developing countries and their flours represent an attractive alternative for the manufacture of gluten free products. In the present study, 4 kidney bean varieties (Alubia, Pallar, Black and Red beans) commonly cultivated in northwestern Argentina, were milled and spontaneously fermented in order to isolate and select autochthonous lactic acid bacteria (LAB) with relevant technological and functional properties for usage as starter cultures. Twelve doughs were fermented with daily back-slopping at 37°C for 6days and evolution of total mesophiles, lactic acid bacteria, and yeasts and molds populations were followed by plate counting...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29177947/effect-of-lactobacillus-rhamnosus-on-the-response-of-galleria-mellonella-against-staphylococcus-aureus-and-escherichia-coli-infections
#9
Adeline Lacerda Jorjão, Felipe Eduardo de Oliveira, Mariella Vieira Pereira Leão, Antonio Olavo Cardoso Jorge, Luciane Dias de Oliveira
This study evaluated the prophylactic effects of the live or heat-killed probiotic strain Lactobacillus rhamnosus ATCC 7469 in Galleria mellonella, inoculated with Staphylococcus aureus or Escherichia coli. L. rhamnosus suspension was prepared and a part of it was autoclaved to obtain heat-killed lactobacilli. The larvae were inoculated of these suspensions and pathogenic. The survival of the larvae was observed during 7 days and after 24 h of inoculation haemocytes counted, melanization and nitric oxide production were analyzed...
November 24, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/29173065/neurobehavioural-effects-of-lactobacillus-rhamnosus-gg-alone-and-in-combination-with-prebiotics-polydextrose-and-galactooligosaccharide-in-male-rats-exposed-to-early-life-stress
#10
Karen-Anne McVey Neufeld, Siobhain M O'Mahony, Alan E Hoban, Rosaline V Waworuntu, Brian M Berg, Timothy G Dinan, John F Cryan
Early life is a period of significant brain development when the brain is at its most plastic and vulnerable. Stressful episodes during this window of development have long-lasting effects on the central nervous system. Rodent maternal separation (MS) is a reliable model of early-life stress and induces alterations in both physiology and behaviour. Intriguingly, the gut microbiota of MS offspring differ from that of non-separated offspring, suggesting a mechanistic role for the microbiota-gut-brain axis. Hence, we tested whether dietary factors known to affect the gut microbiota alter the neurobehavioural effects of MS...
November 27, 2017: Nutritional Neuroscience
https://www.readbyqxmd.com/read/29170992/clinical-strains-of-lactobacillus-reduce-the-filamentation-of-candida-albicans-and-protect-galleria-mellonella-against-experimental-candidiasis
#11
Rodnei Dennis Rossoni, Marisol Dos Santos Velloso, Lívia Mara Alves Figueiredo, Carolina Pistille Martins, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira
Candida albicans is the most common human fungal pathogen and can grow as yeast or filaments, depending on the environmental conditions. The filamentous form is of particular interest because it can play a direct role in adherence and pathogenicity. Therefore, the purpose of this study was to evaluate the effects of three clinical strains of Lactobacillus on C. albicans filamentation as well as their probiotic potential in pathogen-host interactions via an experimental candidiasis model study in Galleria mellonella...
November 23, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29168358/the-effect-of-early-administration-of-combined-multi-strain-and-multi-species-probiotics-on-gastrointestinal-morbidities-and-mortality-in-preterm-infants-a-randomized-controlled-trial-in-a-tertiary-care-unit
#12
İpek Güney-Varal, Nilgün Köksal, Hilal Özkan, Onur Bağcı, Pelin Doğan
Güney-Varal İ, Köksal N, Özkan H, Bağcı O, Doğan P. The effect of early administration of combined multi-strain and multi-species probiotics on gastrointestinal morbidities and mortality in preterm infants: A randomized controlled trial in a tertiary care unit. Turk J Pediatr 2017; 59: 13-19. Necrotizing enterocolitis (NEC) is a gastrointestinal emergency of the neonatal period. The aim of this study was to demonstrate the use of multistrain and multispecies probiotic on gastrointestinal morbidities and mortality...
2017: Turkish Journal of Pediatrics
https://www.readbyqxmd.com/read/29166640/lactic-acid-bacteria-from-andean-grain-amaranth-a-source-of-vitamins-and-functional-value-enzymes
#13
Silvana L Carrizo, Cecilia E Montes de Oca, María Elvira Hébert, Lucila Saavedra, Graciela Vignolo, Jean Guy LeBlanc, Graciela Celestina Rollán
Amaranth is a rediscovered pseudocereal with high nutritional properties. Lactic acid fermentation can increase the functional and nutritional value of foods. The aims of this study were to isolate and evaluate the functionality of lactic acid bacteria (LAB) from amaranth. LAB strains (n = 29) isolated from amaranth sourdough and grains included Lactobacillus (L.) plantarum (n = 8), L. rhamnosus (n = 6), Enterococcus (E.) mundtii (n = 4), E. hermanniensis (n = 3), E. durans (n = 1), Enterococcus sp. (n = 1), Leuconostoc (Lc...
November 23, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29164486/adaptation-and-probiotic-potential-of-lactobacilli-isolated-from-the-oral-cavity-and-intestines-of-healthy-people
#14
Yulia Chervinets, Vyacheslav Chervinets, Boris Shenderov, Ekaterina Belyaeva, Andrey Troshin, Sergey Lebedev, Valery Danilenko
The present study shows that, from 300 Lactobacillus strains isolated from the oral cavity and large intestine of 600 healthy people, only 9 had high antagonistic activity against pathogens and opportunistic pathogens. All antagonistic strains of lactobacilli have been identified by 16S rRNA sequencing and assigned to four species: Lactobacillus fermentum, Lactobacillus rhamnosus, Lactobacillus plantarum, and Lactobacillus casei. In addition, these lactobacilli appeared to be nonpathogenic and had some probiotic potential: the strains produced lactic acid and bacteriocins, showed high sensitivity to broad-spectrum antibiotics, and were capable of forming biofilms in vitro...
November 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29159508/comparative-genomics-and-transcriptome-analysis-of-lactobacillus-rhamnosus-atcc-11443-and-the-mutant-strain-sct-10-10-60-with-enhanced-l-lactic-acid-production-capacity
#15
Liang Sun, Zhilong Lu, Jianxiu Li, Feifei Sun, Ribo Huang
Mechanisms for high L-lactic acid production remain unclear in many bacteria. Lactobacillus rhamnosus SCT-10-10-60 was previously obtained from L. rhamnosus ATCC 11443 via mutagenesis and showed improved L-lactic acid production. In this study, the genomes of strains SCT-10-10-60 and ATCC 11443 were sequenced. Both genomes are a circular chromosome, 2.99 Mb in length with a GC content of approximately 46.8%. Eight split genes were identified in strain SCT-10-10-60, including two LytR family transcriptional regulators, two Rex redox-sensing transcriptional repressors, and four ABC transporters...
November 20, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29132740/effects-of-using-symbiotics-in-the-clinical-nutritional-evolution-of-patients-with-chronic-pancreatitis-study-prospective-randomized-controlled-double-blind
#16
Patricia Quadros Dos Santos, Jorge C Guedes, Rosangela P de Jesus, Raquel Rocha Dos Santos, Rosemeiri L Fiaconne
Patients with chronic pancreatitis (CP) present malabsorption and changes in nutritional status. In this prospective, randomized, controlled, double-blinded, intervention study, developed at the Clinic of Pancreas, we aimed to assess whether the use of symbiotics changes the nutritional status, the biochemical data and the intestinal rate of these patients. The intervention consisted of administering 12 g/day of symbiotics composed of Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium bifidum and fructooligosaccharides to the intervention group and 12 g/day of medium absorption complex carbohydrate to the control group...
April 2017: Clinical Nutrition ESPEN
https://www.readbyqxmd.com/read/29128317/lack-of-efficacy-of-lactobacillus-gg-in-reducing-pulmonary-exacerbations-and-hospital-admissions-in-children-with-cystic-fibrosis-a-randomised-placebo-controlled-trial
#17
Eugenia Bruzzese, Valeria Raia, Eliana Ruberto, Riccardo Scotto, Antonietta Giannattasio, Dario Bruzzese, Maria Cristina Cavicchi, Michela Francalanci, Carla Colombo, Nadia Faelli, Valeria Daccò, Giuseppe Magazzù, Stefano Costa, Vincenzina Lucidi, Fabio Majo, Alfredo Guarino
BACKGROUND: Intestinal dysbiosis has been described in Cystic Fibrosis (CF) and probiotics have been proposed to restore microbial composition. Aim of the study was to investigate the effects of Lactobacillus rhamnosus GG (LGG) on clinical outcomes in children with cystic fibrosis (CF). METHODS: A multicentre, randomised double-blind, clinical trial was conducted in children with CF. After 6months of baseline assessment, enrolled children (2 to 16years of age) received Lactobacillus GG (6×109CFU/day) or placebo for 12months...
November 8, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/29121515/the-genetic-basis-underlying-variation-in-production-of-the-flavour-compound-diacetyl-by-lactobacillus-rhamnosus-strains-in-milk
#18
Raquel Lo, Van Thi Thuy Ho, Nidhi Bansal, Mark S Turner
Diacetyl and the closely related compound acetoin impart desirable buttery flavour and odour to many foods including cheese and are generated through the metabolism of citrate by lactic acid bacteria (LAB). To increase the levels of these compounds, adjunct cultures capable of producing them can be added to cheese fermentations. In this study, we compared the diacetyl and acetoin producing abilities of 13 Lactobacillus rhamnosus strains from cheese sources. Diacetyl and acetoin production was found to be a common feature of Lb...
October 31, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29121411/probiotic-lactobacillus-rhamnosus-gg-prevents-alveolar-bone-loss-in-a-mouse-model-of-experimental-periodontitis
#19
Simona M Gatej, Victor Marino, Richard Bright, Tracy R Fitzsimmons, Neville Gully, Peter Zilm, Rachel J Gibson, Suzanne Edwards, Peter M Bartold
AIM: This study investigated the role of Lactobacillus rhamnosus GG on bone loss and local and systemic inflammation in an in vivo mouse model of experimental periodontitis. MATERIALS AND METHODS: Experimental periodontitis was induced in mice by oral inoculation with Porphyromonas gingivalis and Fusobacterium nucleatum over a period of 44 days. The probiotic LGG was administered via oral inoculation or oral gavage prior to, and during disease induction. The antimicrobial activity of LGG on the inoculum was also tested...
November 9, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/29120368/lactobacillus-bulgaricus-lactobacillus-rhamnosus-and-lactobacillus-paracasei-attenuate-salmonella-enteritidis-salmonella-heidelberg-and-salmonella-typhimurium-colonization-and-virulence-gene-expression-in-vitro
#20
Muhammed Shafeekh Muyyarikkandy, Mary Anne Amalaradjou
Salmonella Enteritidis (SE), Salmonella Typhimurium (ST), and Salmonella Heidelberg (SH) have been responsible for numerous outbreaks associated with the consumption of poultry meat and eggs. Salmonella colonization in chicken is characterized by initial attachment to the cecal epithelial cells (CEC) followed by dissemination to the liver, spleen, and oviduct. Since cecal colonization is critical to Salmonella transmission along the food chain continuum, reducing this intestinal association could potentially decrease poultry meat and egg contamination...
November 9, 2017: International Journal of Molecular Sciences
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