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lactobacillus rhamnosus

Marzia Sichetti, Stefania De Marco, Rita Pagiotti, Giovanna Traina, Donatella Pietrella
OBJECTIVE: In recent years, a great number of studies have been directed toward the evaluation of gastrointestinal microbiota modulation through the introduction of beneficial microorganisms, also known as probiotics. Many studies have highlighted how this category of bacteria is very important for the good development, functioning, and maintenance of our immune system. There is a delicate balance between the immune system, located under the gut epithelial barrier, and the microbiota, but many factors can induce a disequilibrium that leads to an inflammatory state and dysbiosis...
February 14, 2018: Nutrition
Jeong A Kim, Sung-Hak Kim, In Sung Kim, Da Yoon Yu, Sung Chan Kim, Seung Ho Lee, Sang Suk Lee, Cheol-Heui Yun, In Soon Choi, Kwang Keun Cho
Atopic dermatitis is a chronic and recurrent inflammatory skin disease. Recently, probiotics have been shown to suppress allergic symptoms through immunomodulatory responses. In the present study, combinatorial effects on allergic symptoms were identified in BALB/c mice fed with a mixture of four species of probiotics, Bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus plantarum, and sodium butyrate. Following sensitization with whey protein, the mice were challenged and divided into two groups: (1) mice administered with phosphate-buffered saline as a control and (2) mice administered with the probiotic mixture and sodium butyrate...
March 20, 2018: Journal of Medicinal Food
Nwadiuto O Nwamaioha, Salam A Ibrahim
Modified reinforced clostridial medium (mRCM) was developed and evaluated for the differential enumeration of Lactobacillus delbrueckii ssp. bulgaricus. Lactobacillus bulgaricus, an important species of lactic acid bacteria with health benefits, is used in the production of yogurt and other fermented foods. Our results showed that supplementing reinforced clostridial medium with 0.025% CaCl2 , 0.01% uracil, and 0.2% Tween 80 (mRCM) significantly enhanced the growth rate of L. bulgaricus RR and ATCC 11842 strains as measured by the optical densities of these strains after 12 h of incubation at 42°C...
April 4, 2018: Journal of Dairy Science
Justine Guerin, Claire Soligot, Jennifer Burgain, Marion Huguet, Gregory Francius, Sofiane El-Kirat-Chatel, Faustine Gomand, Sarah Lebeer, Yves Le Roux, Frederic Borges, Joël Scher, Claire Gaiani
Milk is the most popular matrix for the delivery of lactic acid bacteria, but little is known about how milk impacts bacterial functionality. Here, the adhesion mechanisms of Lactobacillus rhamnosus GG (LGG) surface mutants to a milk component, the milk fat globule membrane (MFGM), were compared using atomic force microscopy (AFM). AFM results revealed the key adhesive role of the LGG SpaCBA pilus in relation to MFGM. A LGG mutant without exopolysaccharides but with highly exposed pili improved the number of adhesive events between LGG and MFGM compared to LGG wild type (WT)...
March 28, 2018: Colloids and Surfaces. B, Biointerfaces
Gioacchini Giorgia, Ciani Elia, Pessina Andrea, Cecchini Cinzia, Silvi Stefania, Rodiles Ana, Merrifield L Daniel, Olivotto Ike, Carnevali Oliana
In the present study, Nile tilapia Oreochromis niloticus was used as experimental model to study the molecular effects of a new probiotic preparation, Lactogen 13 (Lactobacillus rhamnosus IMC 501® encapsulated with vegetable fat matrices by spray chilling and further indicated as probiotic microgranules), on growth and appetite during larval development. Probiotic microgranules were administered for 30 days to tilapia larvae starting from first feeding. Molecular analysis using high-throughput sequencing revealed that the probiotic could populate the gastrointestinal tract and modulate the microbial communities by significantly increasing the proportion of Lactobacillus as well as reducing the proportion of potential pathogens such as members of the Family Microbacteriaceae, Legionellaceae, and Weeksellaceae...
April 4, 2018: Microbial Ecology
Anna Lorusso, Rossana Coda, Marco Montemurro, Carlo Giuseppe Rizzello
This study aimed at investigating the suitability of quinoa for making yogurt-like beverages. After the selection of the adequate technological parameters, the fermentation was carried out by using different lactic acid bacteria strains: a probiotic ( Lactobacillus rhamnosus SP1), an exopolysaccharides (EPS)-producing ( Weissella confusa DSM 20194), and one isolated from quinoa ( Lactobacillus plantarum T6B10). During the 20 h of fermentation, W. confusa caused the highest viscosity increase. All the strains had improved concentration of free amino acids and γ-Aminobutyric acid (GABA), polyphenols availability, antioxidant activity (up to 54%), and protein digestibility...
April 1, 2018: Foods (Basel, Switzerland)
Qin Xiang Ng, Christina Peters, Nandini Venkatanarayanan, Yan Yih Goh, Collin Yih Xian Ho, Wee-Song Yeo
Urinary tract infections (UTIs) are the most common bacterial infections seen in the community, especially amongst females. The widespread use of antibiotics has led to the increased occurrence of E. coli resistant isolates worldwide. A promising non-antibiotic approach is the use of probiotic lactobacilli strains. This paper hypothesizes that Lactobacillus spp. containing products are able to prevent recurrent urinary tract infections in females. Using the keywords [lactobacillus OR lactobacilli OR probiotic] and [urinary tract infection OR UTI OR cystitis], a preliminary search on the PubMed, Ovid, Google Scholar and ClinicalTrials...
May 2018: Medical Hypotheses
Jonathan W Richter, Gabriella M Shull, John H Fountain, Zhongyuan Guo, Laura P Musselman, Anthony C Fiumera, Gretchen J Mahler
Nanosized titanium dioxide (TiO2 ) is a common additive in food and cosmetic products. The goal of this study was to investigate if TiO2 nanoparticles affect intestinal epithelial tissues, normal intestinal function, or metabolic homeostasis using in vitro and in vivo methods. An in vitro model of intestinal epithelial tissue was created by seeding co-cultures of Caco-2 and HT29-MTX cells on a Transwell permeable support. These experiments were repeated with monolayers that had been cultured with the beneficial commensal bacteria Lactobacillus rhamnosus GG (L...
March 30, 2018: Nanotoxicology
Bin Zhou, Defeng Zhang
Infection following orthopedic surgery is a major complication that can have serious implications on patient health. The present study aimed to investigate the antibacterial effects of bacteriocins obtained from Lactobacillus rhamnosus on a rabbit model of Staphylococcus aureus infection following knee replacement surgery. Blood samples were collected 1, 2, 3, 4 and 5 days after bacteriocin injection, and C-reactive protein (CRP) and interleukin (IL)-6 levels were measured using commercial ELISA kits. In addition, biofilm formation was evaluated by fluorescence microscopy...
March 2018: Experimental and Therapeutic Medicine
Fatih Tornuk, Osman Sagdic, Mehmet Hancer, Hasan Yetim
In this study, a novel procedure was performed for grafting of nanoclays (montmorillonite (MMT) and halloysite (HNT)) with essential oil constituents (thymol (THY), eugenol (EUG) and carvacrol (CRV)) using Tween 80 as surfactant and then the nanoclay particles were incorporated into LLDPE pellets (5 wt%) to produce active nanocomposite films using a twin screw extruder. The resulting nanocomposite films were analyzed for antimicrobial and antioxidant capacity as well as thickness, mechanical, color, barrier, thermal properties and surface morphology and molecular composition...
May 2018: Food Research International
Maryam Pourhajibagher, Abbas Bahador
BACKGROUND: Several microbial species that have been implicated in the pathogenesis of endodontic diseases colonize the infected root canal system. Since the complete removal of endopathogenic agents is essential in endodontic infections therapy, photo-activated disinfection (PAD) is suggested as an alternative method to traditional antimicrobial therapy. Recent studies reported that the molecular methods with low sensitivity and high efficiency to identify fastidious anaerobic endopathogenic microbiota can be replaced by the cultivation-based approaches...
March 22, 2018: Photodiagnosis and Photodynamic Therapy
Lorena Zudaire, Inmaculada Viñas, Lucía Plaza, María Belén Iglesias, Maribel Abadias, Ingrid Aguiló-Aguayo
BACKGROUND: Biological preservation with probiotic bacteria has arisen as an alternative to control the growth of foodborne pathogens on food. The objective of this work was to evaluate the effect of postharvest calcium application and biopreservation with Lactobacillus rhamnosus GG on the quality and bioaccessibility of total phenolic content and antioxidant activity in fresh-cut pears. RESULTS: The immersion of whole pears in a calcium chloride solution did not provide added value...
March 25, 2018: Journal of the Science of Food and Agriculture
Yun Xia, Maixin Lu, Gang Chen, Jianmeng Cao, Fengying Gao, Miao Wang, Zhigang Liu, Defeng Zhang, Huaping Zhu, Mengmeng Yi
The present study aimed to evaluate the individual and combined effects of Lactobacillus rhamnosus (LR) JCM1136 and Lactococcus lactis subsp. lactis (LL) JCM5805 on the growth, intestinal microbiota, intestinal morphology, immune response and disease resistance of juvenile Nile tilapia (Oreochromis niloticus). A total of 720 apparently healthy juvenile Nile tilapia (0.20 ± 0.05 g) were randomly divided into four equal groups. Fish were fed with a basal diet (CK) supplemented with JCM1136 (LR), JCM5805 (LL), and JCM1136 + JCM5805 (LR+LL) at 1 × 108  CFU/g basal diet for 6 weeks, followed by a basal diet for 1 week...
March 14, 2018: Fish & Shellfish Immunology
Guillaume Gobert, Aurélie Cotillard, Candice Fourmestraux, Laurence Pruvost, Jean Miguet, Mickaël Boyer
Analysing correlations between the observed health effects of ingested probiotics and their survival in digestive tract allows adapting their preparations for food. Tracking ingested probiotic in faecal samples requires accurate and specific tools to quantify live vs dead cells at strain level. Traditional culture-based methods are simpler to use but they do not allow quantifying viable but non-cultivable (VBNC) cells and they are poorly discriminant below the species level. We have set up a viable PCR (vPCR) assay combining propidium monoazide (PMA) treatment and either real time quantitative PCR (qPCR) or droplet digital PCR (ddPCR) to quantify a Lactobacillus rhamnosus and two Lactobacillus paracasei subsp...
March 13, 2018: Journal of Microbiological Methods
Shoshannah Eggers, Anna K Barker, Susan Valentine, Timothy Hess, Megan Duster, Nasia Safdar
BACKGROUND: Infection by Staphylococcus aureus (S. aureus) is a major cause of morbidity and mortality. Colonization by S. aureus increases the risk of infection. Little is known about decolonization strategies for S. aureus beyond antibiotics, however probiotics represent a promising alternative. A randomized controlled trial was conducted to determine the efficacy of Lactobacillus rhamnosus (L. rhamnosus) HN001 in reducing carriage of S. aureus at multiple body sites. METHODS: One hundred thirteen subjects, positive for S...
March 14, 2018: BMC Infectious Diseases
Yanina Kolling, Susana Salva, Julio Villena, Susana Alvarez
Previously, we reported that Lactobacillus rhamnosus CRL1505 peptidoglycan (PG05) improves the innate immune response in immunocompromised-malnourished mice after Streptococcus pneumoniae infection. This study extends those previous findings by demonstrating that the dietary recovery of malnourished mice with nasal administration of PG05 improves not only the innate immune response but the respiratory and systemic adaptive humoral response as well. PG05 enhanced the Th2 response, the recovery of B cells, and the concentration and opsonophagocytic activity of anti-pneumococcal antibodies...
2018: PloS One
Jason W Arnold, Joshua B Simpson, Jeffrey Roach, Jakub Kwintkiewicz, M Andrea Azcarate-Peril
Large-scale microbiome studies have established that most of the diversity contained in the gastrointestinal tract is represented at the strain level; however, exhaustive genomic and physiological characterization of human isolates is still lacking. With increased use of probiotics as interventions for gastrointestinal disorders, genomic and functional characterization of novel microorganisms becomes essential. In this study, we explored the impact of strain-level genomic variability on bacterial physiology of two novel human Lactobacillus rhamnosus strains (AMC143 and AMC010) of probiotic potential in relation to stress resistance...
2018: Frontiers in Microbiology
Jiayi Cai, Chunyang Zhao, Yajie Du, Yaqiong Zhang, Mingyi Zhao, Qingchun Zhao
Background: Probiotics are commonly used for the prevention of antibiotic-associated diarrhea (AAD). However, the optimum regimen remains controversial. Objective: The objective of this article is to compare and rank the relative efficacy and tolerability among all available probiotic agents for AAD through a network meta-analysis. Methods: Eligible studies were identified by searching PubMed, Embase, Medline, Cochrane library and Web of Science for randomized controlled trials (RCTs) that examined the efficacy of probiotic therapy for AAD...
March 2018: United European Gastroenterology Journal
Teresa Marggraf, Petra Ganas, Sebastian Paris, Falk Schwendicke
Sealing can arrest caries lesions. We aimed to evaluate if sealing effects and kinetics are bacterial-strain and sealing-material specific. Human dentin discs were mounted in a dual-chamber device. Caries lesions were induced chemically and contaminated with either Lactobacillus rhamnosus (LR) or Streptococcus sobrinus (SS). For (1) kinetics assessment, the initial bacterial load and the sealing period were varied, and lesions sealed using a self-etch adhesive and composite. For (2) comparing materials, six sealing protocols (#1-#6) were evaluated: 1# Self-etch adhesive plus composite placed without a liner, or #2 calcium hydroxide, or #3 mineral trioxide aggregate, or #4 Biodentine liners; #5 antibacterial adhesive plus composite; #6 glass ionomer cement...
February 28, 2018: Scientific Reports
Diane G Carnathan, Joseph J Mackel, Shelby L Sweat, Chiamaka A Enemuo, Etse H Gebru, Pallavi Dhadvai, Sailaja Gangadhara, Sakeenah Hicks, Thomas H Vanderford, Rama R Amara, José Esparza, Wei Lu, Jean-Marie Andrieu, Guido Silvestri
A major obstacle to development of an effective AIDS vaccine is that along with intended beneficial responses, immunization regimen may activate CD4+ T cells that can facilitate acquisition of HIV by serving as target cells for the virus. Lu et al. reported that intra-gastric administration of chemically inactivated SIVmac239 (iSIV) and Lactobacillus plantarum (LP) (iSIV+LP) protected 15/16 Chinese-origin rhesus macaques (RMs) from high-dose intra-rectal SIVmac239 challenge at three months post-immunization...
February 28, 2018: Journal of Virology
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