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https://www.readbyqxmd.com/read/29449386/draft-genome-sequence-of-lactobacillus-rhamnosus-nrrl-b-442-a-potential-probiotic-strain
#1
Muhammed Shafeekh Muyyarikkandy, Fahad H Alqahtani, Ion Mandoiu, Mary Anne Amalaradjou
Lactic acid bacteria are known to exhibit probiotic properties through various mechanisms, including competitive exclusion, pathogen inhibition, production of antimicrobial substances, and maintenance of eubiosis. Here, we present the draft genome sequence of a novel probiotic strain, Lactobacillus rhamnosus strain NRRL B-442, which exhibits potent antivirulence activity against Salmonella enterica .
February 15, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29446057/in-vitro-evaluation-of-probiotic-potential-of-lactic-acid-bacteria-isolated-from-yunnan-de-ang-pickled-tea
#2
Zhenhui Cao, Hongbin Pan, Shijun Li, Chongying Shi, Sifan Wang, Fuyi Wang, Pengfei Ye, Junjing Jia, Changrong Ge, Qiuye Lin, Zhiyong Zhao
This study aimed to investigate the probiotic potential of lactic acid bacteria (LAB) strains isolated from De'ang pickled tea, a traditional food consumed by the De'ang nationality of Yunnan, China. Twenty-six LAB strains isolated from De'ang pickled tea were subjected to identification based on 16S rRNA gene sequence analysis. Twenty-four belonged to Lactobacillus plantarum, one belonged to Enterococcus casseliflavus, and one belonged to Lactobacillus acidophilus. Eighteen out of 26 LAB strains which showed a higher capability to tolerate simulated gastrointestinal juices were chosen to further evaluate their probiotic properties...
February 14, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29444150/probiotic-and-anti-inflammatory-potential-of-lactobacillus-rhamnosus-4b15-and-lactobacillus-gasseri-4m13-isolated-from-infant-feces
#3
Nam Su Oh, Jae Yeon Joung, Ji Young Lee, Younghoon Kim
A total of 22 Lactobacillus strains, which were isolated from infant feces were evaluated for their probiotic potential along with resistance to low pH and bile salts. Eight isolates (L. reuteri 3M02 and 3M03, L. gasseri 4M13, 4R22, 5R01, 5R02, and 5R13, and L. rhamnosus 4B15) with high tolerance to acid and bile salts, and ability to adhere to the intestine were screened from 22 strains. Further, functional properties of 8 Lactobacillus strains, such as anti-oxidation, inhibition of α-glucosidase activity, cholesterol-lowering, and anti-inflammation were evaluated...
2018: PloS One
https://www.readbyqxmd.com/read/29442170/surface-proteins-involved-in-the-adhesion-of-streptococcus-salivarius-to-human-intestinal-epithelial-cells
#4
Fanny Chaffanel, Florence Charron-Bourgoin, Claire Soligot, Mounira Kebouchi, Stéphane Bertin, Sophie Payot, Yves Le Roux, Nathalie Leblond-Bourget
The adhesion properties of 14 Streptococcus salivarius strains to mucus (HT29-MTX) and non-mucus secreting (Caco-2/TC7) human intestinal epithelial cells were investigated. Ability to adhere to these two eukaryotic cell lines greatly differs between strains. The presence of mucus played a major factor in adhesion, likely due to high adhesiveness to mucins present in the native human mucus layer covering the whole cell surface. Only one S. salivarius strain (F6-1), isolated from the feces of a healthy baby, was found to strongly adhere to HT-29 MTX cells at a level comparable to that of Lactobacillus rhamnosus GG, a probiotic strain considered to be highly adherent...
February 13, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29434069/vaginal-lactobacilli-profile-in-pregnant-women-with-normal-abnormal-vaginal-flora
#5
Thirupathaiah Yeruva, Hemalatha Rajkumar, Vasundhara Donugama
Background & objectives: Lactobacilli species that are better adapted to vaginal environment of women may colonize better and offer protection against vaginal pathogenic bacteria. In this study, the distribution of common Lactobacillus species was investigated in pregnant women. Methods: Sixty seven pregnant women were included in the study and vaginal samples were collected for Gram staining. Women were classified as normal vaginal flora, intermediate flora and bacterial vaginosis (BV) based on Nugent's score...
October 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29428745/technological-characterization-of-lactobacillus-in-semihard-artisanal-goat-cheeses-from-different-mediterranean-areas-for-potential-use-as-nonstarter-lactic-acid-bacteria
#6
Zhaoxu Meng, Lanwei Zhang, Liang Xin, Kai Lin, HuaXi Yi, Xue Han
The potential of 25 Lactobacillus isolates from 8 semihard artisanal goat cheeses manufactured in 4 different Mediterranean areas was examined for use as nonstarter lactic acid bacteria. The isolates were identified using 16S rDNA sequence analysis. Sixteen strains belonged to Lactobacillus paracasei and 9 to Lactobacillus rhamnosus. The isolates were first screened for salt tolerance, exopolysaccharide and diacetyl production, proteolytic and lipolytic activity, and acidification and autolyzing capacities...
February 7, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29427224/chemoprevention-by-probiotics-during-1-2-dimethylhydrazine-induced-colon-carcinogenesis-in-rats
#7
Sohini Walia, Rozy Kamal, D K Dhawan, S S Kanwar
BACKGROUND: Probiotics are believed to have properties that lower the risk of colon cancer. However, the mechanisms by which they exert their beneficial effects are relatively unknown. AIM: To assess the impact of probiotics in preventing induction of colon carcinogenesis in rats. METHODS: The rats were divided into six groups viz., normal control, Lactobacillus plantarum (AdF10)-treated, Lactobacillus rhamnosus GG (LGG)-treated, 1,2-dimethylhydrazine (DMH)-treated, L...
February 9, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29417378/in-vitro-screening-of-indigenous-lactobacillus-isolates-for-selecting-organisms-with-better-health-promoting-attributes
#8
Hemanti Mandal, Tamishraha Bagchi
Lactobacilli have several attributes that provide health benefits to the host. The aim of this study was to screen indigenous lactobacilli from human gut and fermented foods for such attributes as production of β- and α-galactosidase and also their ability to reduce serum cholesterol. Lactobacilli were cultured on MRS broth and β-galactosidase activity was determined using o-nitrophenyl-β-D-galactopyranoside (ONPG) as a substrate. Three isolates Lactobacillus fermentum GPI-3 and L. fermentum GPI-6 and Lactobacillus salivarius GPI-1(S) showed better β-galactosidase activity than the standard strains Lactobacillus rhamnosus GG (LGG) and Lactobacillus plantarum ATCC 8014...
February 8, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29417300/stress-influenced-the-aerotolerance-of-lactobacillus-rhamnosus-hsryfm-1301
#9
Chenchen Zhang, Jingyu Lu, Duo Yang, Xia Chen, Yujun Huang, Ruixia Gu
OBJECTIVE: To investigate the aerotolerance of Lactobacillus rhamnosus hsryfm 1301 and its influencing factors. RESULTS: The growth rate of L. rhamnosus hsryfm 1301 weakened noticeably when the concentration of supplemented H2O2 reached 1 mM, and only 2% of all L. rhamnosus hsryfm 1301 cells survived in MRS broth supplemented with 2 mM H2O2 for 1 h. After pretreatment with 0.5 mM H2O2, the surviving cells of L. rhamnosus hsryfm 1301 in the presence of 5 mM H2O2 for 1 h increased from 3...
February 7, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29415330/maternal-supplementation-alone-with-l-rhamnosus-hn001-during-pregnancy-and-breastfeeding-does-not-reduce-infant-eczema
#10
Kristin Wickens, Christine Barthow, Edwin A Mitchell, Thorsten V Stanley, Gordon Purdie, Judy Rowden, Janice Kang, Fiona Hood, Lieke van den Elsen, Elizabeth Forbes-Blom, Isobel Franklin, Phillipa Barnes, Penny Fitzharris, Robyn M Maude, Peter Stone, Peter Abels, Rinki Murphy, Julian Crane
BACKGROUND: In a randomised placebo-controlled trial, we previously found that the probiotic Lactobacillus rhamnosus HN001 (HN001) taken by mothers from 35 weeks gestation until 6 months post-partum if breastfeeding and their child from birth to age 2 years halved the risk of eczema during the first 2 years of life. We aimed to test whether maternal supplementation alone is sufficient to reduce eczema, and compare this to our previous study when both mothers and their child were supplemented...
February 7, 2018: Pediatric Allergy and Immunology
https://www.readbyqxmd.com/read/29409331/lactobacillus-salivarius-la307-and-lactobacillus-rhamnosus-la305-attenuate-skin-inflammation-in-mice
#11
S Holowacz, C Blondeau, I Guinobert, A Guilbot, S Hidalgo, J F Bisson
Oral probiotics potential for the management of dermatological diseases is vast. However, results of available studies in skin diseases, such as atopic dermatitis (AD), are inconsistent, partly because probiotic effects are strain specific. Careful selection of probiotic strains is therefore indispensable to ensure efficacy of treatment. In this study, Lactobacillus salivarius LA307, Lactobacillus rhamnosus LA305 and Bifidobacterium bifidum PI22, three strains that were previously identified for their interesting immunomodulatory properties in allergy and/or colitis models, were assessed in the prevention of chronic skin inflammation induced by repeated applications of 12-O-tetradecanoylphorbol-13-acetate in hairless SKH-1 mice...
February 7, 2018: Beneficial Microbes
https://www.readbyqxmd.com/read/29402455/routine-supplementation-of-lactobacillus-rhamnosus-gg-and-risk-of-necrotizing-enterocolitis-in-very-low-birth-weight-infants
#12
Andrea F Kane, Anisha D Bhatia, Patricia W Denning, Andi L Shane, Ravi Mangal Patel
OBJECTIVE: To evaluate if routine supplementation of Lactobacillus rhamnosus GG ATCC 53103 (LGG) is associated with a decreased risk of necrotizing enterocolitis in very low birth weight (VLBW) infants. STUDY DESIGN: Retrospective observational cohort study of VLBW (<1500 g) infants at a single center from 2008 to 2016. LGG supplementation with Culturelle at a dose of 2.5 to 5 × 109 CFU/day began in 2014. We used multivariable logistic regression to evaluate the association between LGG supplementation and necrotizing enterocolitis (modified Bell stage IIA or greater), after adjusting for potential confounders...
February 2, 2018: Journal of Pediatrics
https://www.readbyqxmd.com/read/29393319/probiotic-attributes-and-prevention-of-lps-induced-pro-inflammatory-stress-in-raw264-7-macrophages-and-human-intestinal-epithelial-cell-line-caco-2-by-newly-isolated-weissella-cibaria-strains
#13
Shashank Singh, Ruchika Bhatia, Ankit Singh, Paramdeep Singh, Ramandeep Kaur, Pragyanshu Khare, Ravi K Purama, Ravneet K Boparai, Praveen Rishi, Padma Ambalam, Sanjay Kumar Bhadada, Mahendra Bishnoi, Jaspreet Kaur, Kanthi Kiran Kondepudi
Probiotic lactic acid bacteria are known to modulate gut associated immune responses. Not many studies have reported on the role of Weissella species in preventing lipopolysaccharide (LPS) induced proinflammatory stress in murine macrophages as well as in human intestinal epithelial cells (Caco-2). Therefore, the present study was taken up to evaluate the probiotic attributes of four newly isolated Weissella strains (two each from fermented dosa batter and a human infant faecal sample); these attributes are cholesterol reduction, adhesion to Caco-2 cells and mucin and their ability to prevent LPS-induced nitric oxide and proinflammatory cytokine (IL-6, IL-1β and TNFα) production by the murine macrophages and IL-8 production by the human epithelial cells...
February 2, 2018: Food & Function
https://www.readbyqxmd.com/read/29391756/effect-of-lactobacillus-rhamnosus-gg-supernatant-on-serotonin-transporter-expression-in-rats-with-post-infectious-irritable-bowel-syndrome
#14
Ya-Nan Cao, Li-Juan Feng, Yuan-Yuan Liu, Kui Jiang, Mao-Jun Zhang, Yi-Xin Gu, Bang-Mao Wang, Jia Gao, Ze-Lan Wang, Yu-Ming Wang
AIM: To evaluate the effect of Lactobacillus rhamnosus GG supernatant (LGG-s) on the expression of serotonin transporter (SERT) in rats with post-infectious irritable bowel syndrome (PI-IBS). METHODS: Campylobacter jejuni 81-176 (1010 CFU/mL) was used to induce intestinal infection to develop a PI-IBS model. After evaluation of the post-infectious phase by biochemical tests, DNA agarose gel electrophoresis, abdominal withdrawal reflex (AWR) test, and the intestinal motility test, four PI-IBS groups received different concentrations of LGG-s for 4 wk...
January 21, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29391694/effect-of-probiotic-supplementation-on-organic-feed-to-alternative-antibiotic-growth-promoter-on-production-performance-and-economics-analysis-of-quail
#15
W P Lokapirnasari, A R Dewi, A Fathinah, S Hidanah, N Harijani, Soeharsono, B Karimah, A D Andriani
Aim: The purpose of this study was to know the production performance and economic analysis in quail which use probiotic supplementation to alternate antibiotic growth promoter (AGP) to feed consumption, water consumption, egg production, egg mass, feed conversion, and feed efficiency. Materials and Methods: About 240 quails (Coturnix coturnix japonica) at 14 weeks of age were completely randomized into four treatments, each treatment consisted of six replications and each replication consisted by 10 heads...
December 2017: Veterinary World
https://www.readbyqxmd.com/read/29390976/probiotics-and-infective-endocarditis-in-patients-with-hereditary-hemorrhagic-telangiectasia-a-clinical-case-and-a-review-of-the-literature
#16
Evangelo Boumis, Alessandro Capone, Vincenzo Galati, Carolina Venditti, Nicola Petrosillo
BACKGROUND: In the last decades, probiotics have been widely used as food supplements because of their putative beneficial health effects. They are generally considered safe but rare reports of serious infections caused by bacteria included in the definition of probiotics raise concerns on their potential pathogenic role in patients with particular predisposing factors. Patients with hereditary hemorrhagic telangiectasia (HHT) are exposed to infections because of telangiectasias and arteriovenous malformations (AVMs)...
February 1, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29389591/optimization-of-culture-conditions-for-gamma-aminobutyric-acid-production-in-fermented-adzuki-bean-milk
#17
Hung Yi Song, Roch Chui Yu
γ-Aminobutyric acid (GABA), a nonprotein amino acid, is widely distributed in nature and fulfills several physiological functions. In this study, various lactic acid strains commonly used to produce fermented milk products were inoculated into adzuki bean milk for producing GABA. The high GABA producing strain was selected in further experiment to improve the GABA production utilizing culture medium optimization. The results demonstrated that adzuki bean milk inoculated with Lactobacillus rhamnosus GG increased GABA content from 0...
January 2018: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/29387999/root-caries-prevention-via-sodium-fluoride-chlorhexidine-and-silver-diamine-fluoride-in-vitro
#18
Gerd Göstemeyer, Anna Kohls, Sebastian Paris, Falk Schwendicke
Uncertainty exists as to how to best prevent root caries development. The aim of the present study was to compare sodium fluoride (NaF), chlorhexidine (CHX) and silver diamine fluoride (SDF) varnishes (V) and rinses (R) regarding their caries preventive effect in an artificial caries biofilm model. 140 bovine root dentin samples were cut, polished and embedded. Samples were allocated to seven treatment groups (n = 20/group): Four varnishes (applied once prior biofilm challenge): 38% SDF (SDFV), 35% CHX-varnish (CHXV), 22,600 ppm NaF-varnish (NaFV), placebo-varnish (PV); two rinses (applied once daily during biofilm challenge): 500 ppm NaF solution (NaFR), 0...
January 31, 2018: Odontology
https://www.readbyqxmd.com/read/29383669/probiotics-for-prevention-and-treatment-of-clostridium-difficile-infection
#19
Lorena Valdés-Varela, Miguel Gueimonde, Patricia Ruas-Madiedo
Probiotics have been claimed as a valuable tool to restore the balance in the intestinal microbiota following a dysbiosis caused by, among other factors, antibiotic therapy. This perturbed environment could favor the overgrowth of Clostridium difficile and, in fact, the occurrence of C. difficile-associated infections (CDI) is being increasing in recent years. In spite of the high number of probiotics able to in vitro inhibit the growth and/or toxicity of this pathogen, its application for treatment or prevention of CDI is still scarce since there are not enough well-defined clinical studies supporting efficacy...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29380647/antifungal-activity-of-clinical-lactobacillus-strains-against-candida-albicans-biofilms-identification-of-potential-probiotic-candidates-to-prevent-oral-candidiasis
#20
Rodnei Dennis Rossoni, Patrícia Pimentel de Barros, Janaina Araújo de Alvarenga, Felipe de Camargo Ribeiro, Marisol Dos Santos Velloso, Beth Burgwyn Fuchs, Eleftherios Mylonakis, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira
This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation...
January 30, 2018: Biofouling
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