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https://www.readbyqxmd.com/read/28412527/aquaporins-as-potential-drought-tolerance-inducing-proteins-toward-instigating-stress-tolerance
#1
Sajad Majeed Zargar, Preeti Nagar, Rupesh Deshmukh, Muslima Nazir, Aijaz Ahmad Wani, Khalid Zaffar Masoodi, Ganesh Kumar Agrawal, Randeep Rakwal
Aquaporins (AQPs) are primarily involved in maintaining cellular water homeostasis. Their role in diverse physiological processes has fascinated plant scientists for more than a decade, particularly concerning abiotic stresses. Increasing examples of evidence in various crop plants indicate that the AQPs are responsible for precise regulation of water movement and consequently play a crucial role in the drought stress tolerance. Since drought is one of the major abiotic stresses affecting agricultural production worldwide, it has become a critical agenda to focus research on the development of drought tolerant crop plants...
April 13, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28405801/urine-exosomes-from-healthy-and-hypertensive-pregnancies-display-elevated-level-of-%C3%AE-subunit-and-cleaved-%C3%AE-and-%C3%AE-subunits-of-the-epithelial-sodium-channel-enac
#2
Maria R Nielsen, Britta Frederiksen-Møller, Rikke Zachar, Jan S Jørgensen, Mie R Hansen, Rikke Ydegaard, Per Svenningsen, Kristian Buhl, Boye L Jensen
Preeclampsia is characterized by hypertension, proteinuria, suppression of plasma renin-angiotensin-aldosterone, and impaired urine sodium excretion. Aberrantly filtered plasmin in urine may activate proteolytically the γ-subunit of the epithelial sodium channel (ENaC) and promote Na(+) reabsorption and urine K(+) loss. Plasma and urine was sampled from patients with preeclampsia, healthy pregnant controls and non-pregnant women, and from patients with nephrostomy catheters. Aldosterone concentration, urine plasminogen, and protein were determined...
April 12, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28399644/effects-of-gonadectomy-and-testosterone-treatment-on-aquaporin-expression-in-the-kidney-of-normotensive-and-hypertensive-rats
#3
Su Yi Loh, Nelli Giribabu, Naguib Salleh
We tested the hypothesis that testosterone-induced increase in blood pressure was due to changes in aquaporin (AQP) expression in kidneys. In this study, expression level of kidney AQPs was investigated under testosterone influence. Adult normotensive Wistar Kyoto (WKY) and hypertensive SHR male and female rats underwent gonadectomy. For female rats, testosterone was given for six weeks duration, two weeks following ovariectomy via subcutaneous silastic implant. Mean arterial pressure (MAP) was measured in all the rats after eight weeks via carotid artery cannulation and the rats were then sacrificed and kidneys were harvested for analyses of AQP-1, 2, 3, 4, 6, and 7 mRNA and protein expressions by quantitative real-time PCR and Western blotting, respectively...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28396190/protective-effect-of-4-methoxy-benzyl-alcohol-on-the-blood-brain-barrier-after-cerebral-ischemia-reperfusion-injury
#4
Fangyan He, Xiaohua Duan, Rong Dai, Yan Li, Qing Lin
Damage of the blood-brain barrier (BBB) during the process of cerebral ischemic injury is a key factor that influences the therapeutic efficacy to the cerebral ischemic injury. This work was designed to investigate the mechanisms underlying the protective effects of 4-methoxy benzyl alcohol (4-MA) on the BBB by developing a cerebral ischemia/reperfusion model of rats (MCAO/R). The MCAO/R was developed through a thread embolism method. The neurologic scales, the brain infarct rate, and the Evans blue (EB) contents of the brains were detected...
April 7, 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/28390076/sugar-and-hexokinase-suppress-expression-of-pip-aquaporins-and-reduce-leaf-hydraulics-that-preserves-leaf-water-potential
#5
Gilor Kelly, Nir Sade, Adi Doron-Faigenboim, Stephen Lerner, Arava Shatil-Cohen, Yelena Yeselson, Aiman Egbaria, Jayaram Kottapalli, Arthur A Schaffer, Menachem Moshelion, David Granot
Sugars affect central aspects of plant physiology, including photosynthesis, stomatal behavior and the loss of water through the stomata. Yet, the potential effects of sugars on plant aquaporins (AQPs) and water conductance have not been examined. We used database and transcriptional analyses, as well as cellular and whole-plant functional techniques to examine the link between sugar-related genes and AQPs. Database analyses revealed a high level of correlation between the expression of AQPs and that of sugar-related genes, including the Arabidopsis hexokinases 1 (AtHXK1)...
April 8, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28380294/improving-permeation-and-antifouling-performance-of-polyamide-nanofiltration-membranes-through-the-incorporation-of-arginine
#6
Lin Fan, Qi Zhang, Zhen Yang, Runnan Zhang, Ya-Nan Liu, Mingrui He, Zhongyi Jiang, Yanlei Su
Inspired by the hydrophilicity effect of arginine (Arg) in water channel aquaporins (AQPs), Arg was incorporated into the polyamide layer during interfacial polymerization to enhance the permeation and antifouling performance of the nanofiltration (NF) membranes. Due to the presence of active amine groups, Arg became another aqueous phase monomer along with piperazine (PIP) to react with trimesoyl chloride (TMC) during interfacial polymerization, which was incorporated into the polyamide network. The resulting polyamide NF membranes were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), atomic force microscopy (AFM), static water contact angle, zeta potential, and positron annihilation spectroscopy (PAS) measurement...
April 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28377226/aquaporin-3-facilitates-tumor-growth-in-pancreatic-cancer-by-modulating-mtor-signaling
#7
Xunwei Huang, Li Huang, Minhua Shao
Aquaporins (AQP) have been demonstrated to be dysregulated in many human cancers and is thought to be involved in pancreatic carcinogenesis and progression. However, the oncogenic roles and underlying mechanism of AQP in pancreatic ductal adenocarcinoma (PDAC) remain largely unknown. In this study, by data mining of TCGA dataset and CCLE database, we identified that AQP3 is the major AQP expressed in PDAC. Then, the microRNA-874, was demonstrated to be a key regulator of AQP3 expression in PDAC cells. Genetic silencing of AQP3 expression had pronounced effects on cell proliferation and apoptosis of the PDAC cell lines BXPC3 and HPAFII...
April 1, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28370569/aquaporin-9-is-expressed-in-the-epididymis-of-immature-and-mature-pigs
#8
B C Schimming, Cae Baumam, Pff Pinheiro, R de Matteis, R F Domeniconi
Aquaporins (AQPs) are channel proteins that facilitate the transepithelial and bidirectional movement of water. AQP9 is an aquaporin that is expressed in the mammalian epididymis. This water transport contributes to epididymal sperm concentration. This study aimed to examine the morphology of epididymal epithelium in piglets and boars, as well as the expression and immunolocalization of AQP9. The piglets presented an epididymal epithelium in differentiation with principal, basal and apical cells. The cellular population of the epididymal epithelium in boars consisted of principal, basal, apical, clear and narrow cells...
March 30, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28360107/electrostatic-attraction-of-weak-monoacid-anions-increases-probability-for-protonation-and-passage-through-aquaporins
#9
Monja Rothert, Deike Rönfeldt, Eric Beitz
A positive electrostatic field emanating from the center of the aquaporin (AQP) water and solute channel is responsible for the repulsion of cations. At the same time, however, a positive field will attract anions. In this regard, l-lactate/lactic acid permeability has been shown for various isoforms of the otherwise highly water and neutral substrate selective AQP family. The structural requirements rendering certain AQPs permeable for weak monoacids and the mechanism of conduction have remained unclear. Here, we show by profiling pH-dependent substrate permeability, measurements of media alkalization, and proton decoupling, that AQP9 acts as a channel for the protonated, neutral monocarboxylic acid species...
March 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28358927/aquaglyceroporin-null-trypanosomes-display-glycerol-transport-defects-and-respiratory-inhibitor-sensitivity
#10
Laura Jeacock, Nicola Baker, Natalie Wiedemar, Pascal Mäser, David Horn
Aquaglyceroporins (AQPs) transport water and glycerol and play important roles in drug-uptake in pathogenic trypanosomatids. For example, AQP2 in the human-infectious African trypanosome, Trypanosoma brucei gambiense, is responsible for melarsoprol and pentamidine-uptake, and melarsoprol treatment-failure has been found to be due to AQP2-defects in these parasites. To further probe the roles of these transporters, we assembled a T. b. brucei strain lacking all three AQP-genes. Triple-null aqp1-2-3 T. b. brucei displayed only a very moderate growth defect in vitro, established infections in mice and recovered effectively from hypotonic-shock...
March 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28351378/melatonin-alleviates-brain-and-peripheral-tissue-edema-in-a-neonatal-rat-model-of-hypoxic-ischemic-brain-damage-the-involvement-of-edema-related-proteins
#11
Li-Xiao Xu, Yuan Lv, Yan-Hong Li, Xin Ding, Ying Wang, Xing Han, Ming-Hua Liu, Bin Sun, Xing Feng
BACKGROUND: Previous studies have indicated edema may be involved in the pathophysiology following hypoxic-ischemic encephalopathy (HIE), and melatonin may exhibit neuro-protection against brain insults. However, little is known regarding the mechanisms that involve the protective effects of melatonin in the brain and peripheral tissues after HIE. The present study aimed to examine the effects of melatonin on multiple organs, and the expression of edema related proteins in a neonatal rat model of hypoxic-ischemic brain damage (HIBD)...
March 28, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28341573/quercetin-alters-uterine-fluid-volume-and-aquaporin-aqp-subunits-aqp-1-2-5-7-expression-in-the-uterus-in-the-presence-of-sex-steroids-in-rats
#12
Huma Shahzad, Nelli Giribabu, Kamarulzaman Karim, Sekaran Muniandy, Normadiah M Kassim, Naguib Salleh
Effects of quercetin on uterine fluid volume and aquaporin (AQP) expression in the uterus were investigated. Estradiol (E) or estradiol followed by progesterone (E+P) were given to ovariectomised rats with or without quercetin (10, 50 or 100mg/kg/day) treatment. Uteri were harvested and its inner/outer circumference ratio was determined. AQP-1, 2, 5 and 7 mRNA and protein levels in uterus were quantified by Real-time PCR and Western blotting respectively. Protein distribution was observed by immunohistochemistry...
March 22, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28335427/laxative-effects-of-total-diterpenoids-extracted-from-the-roots-of-euphorbia-pekinensis-are-attributable-to-alterations-of-aquaporins-in-the-colon
#13
Kuilong Wang, Lian Liu, Jianyu Huang, Hongli Yu, Hao Wu, Yu Duan, Xiaobing Cui, Xingde Zhang, Liping Liu, Wei Wang
This study was designed to evaluate the toxic effects of total diterpenoids extracted from the roots of Euphorbia pekinensis (TDEP) on the mouse colon and to clarify the mechanism. Dried powdered roots of E. pekinensis were extracted with chloroform, and then the extract (6.7 g) was subjected to column chromatography and preparative TLC, giving TDEP. Using the HPLC-DAD method, the purity of TDEP was determined as 85.26%. Mice were orally administered with TDEP (3.942, 19.71 and 39.42 mg/kg), after which fecal water content and colon water content were examined...
March 14, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28318025/maternal-chronic-hypoxia-increases-expression-of-genes-regulating-lung-liquid-movement-and-surfactant-maturation-in-male-fetuses-in-late-gestation
#14
Erin V McGillick, Sandra Orgeig, Beth J Allison, Kirsty L Brain, Youguo Niu, Nozomi Itani, Katie L Skeffington, Andrew D Kane, Emilio A Herrera, Dino A Giussani, Janna L Morrison
Chronic fetal hypoxaemia is a common pregnancy complication that may arise from maternal, placental and/or fetal factors. Respiratory outcome of the infant at birth likely depends on the duration, timing and severity of the hypoxaemic insult. We have isolated the effect of maternal chronic hypoxia (MCH) for a month in late gestation on fetal lung development. Pregnant ewes were exposed to normoxia (21% O2 ) or hypoxia (10% O2 ) from 105-138 d gestation (term ∼145 d). At 138 d, gene expression in fetal lung tissue was determined by qRT-PCR...
March 20, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28297654/role-of-pore-lining-residues-in-defining-the-rate-of-water-conduction-by-aquaporin-0
#15
Patrick O Saboe, Chiara Rapisarda, Shreyas Kaptan, Yu-Shan Hsiao, Samantha R Summers, Rita De Zorzi, Danijela Dukovski, Jiaheng Yu, Bert L de Groot, Manish Kumar, Thomas Walz
Compared to other aquaporins (AQPs), lens-specific AQP0 is a poor water channel, and its permeability was reported to be pH-dependent. To date, most water conduction studies on AQP0 were performed on protein expressed in Xenopus oocytes, and the results may therefore also reflect effects introduced by the oocytes themselves. Experiments with purified AQP0 reconstituted into liposomes are challenging because the water permeability of AQP0 is only slightly higher than that of pure lipid bilayers. By reconstituting high amounts of AQP0 and using high concentrations of cholesterol to reduce the permeability of the lipid bilayer, we improved the signal-to-noise ratio of water permeability measurements on AQP0 proteoliposomes...
March 14, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28295351/when-size-matters-transient-receptor-potential-vanilloid-4-channel-as-a-volume-sensor-rather-than-an-osmo-sensor
#16
Trine L Toft-Bertelsen, David Krízaj, Nanna MacAulay
Transient receptor potential channel 4 of the vanilloid subfamily (TRPV4) is activated by a diverse range of molecular cues, such as heat, lipid metabolites, synthetic agonists, in addition to hyposmotic challenges. As a non-selective cation channel permeable to Ca(2+) , it transduces physical stress in the form of osmotic cell swelling into intracellular Ca(2+) -dependent signaling events. Its contribution to cell volume regulation might include interactions with aquaporin (AQP) water channel isoforms, although the proposed requirement for a TRPV4-AQP4 macromolecular complex remains to be resolved...
March 10, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28286329/aquaporin-in-different-moult-stages-of-a-freshwater-decapod-crustacean-expression-and-participation-in-muscle-hydration-control
#17
Kamila Foguesatto, Robert T Boyle, Monique T Rovani, Carolina A Freire, Marta M Souza
Crustaceans, during their moult cycle, at the stages of both pre-moult and post-moult, need water uptake. This movement of water creates a challenge for the regulation of cell volume. The cells of freshwater decapods require a high regulatory capacity to deal with hyposmotic stresses, given the need to face dilution of the haemolymph during their moult cycles. This study investigated the variation in the expression of water channels (aquaporins) along the moult cycle of a freshwater palaemonid shrimp, focusing on their role in cell volume regulation...
March 9, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28283882/aquaporin-4-as-a-new-target-against-methamphetamine-induced-brain-alterations-focus-on-the-neurogliovascular-unit-and-motivational-behavior
#18
Ricardo Alexandre Leitão, José Sereno, João Miguel Castelhano, Sónia Isabel Gonçalves, Vanessa Coelho-Santos, Carlos Fontes-Ribeiro, Miguel Castelo-Branco, Ana Paula Silva
Methamphetamine (METH) abuse/misuse is a worldwide problem, and despite extensive characterization of its neurotoxicity over the last years, many questions remain unanswered. Recently, it was shown that METH compromises the blood-brain barrier (BBB) and causes a disturbance in the water homeostasis leading to brain edema. Importantly, water transport at BBB is regulated by water channels, aquaporins (AQPs), with AQP4 being expressed in astrocytic end-feet surrounding brain endothelium. Thus, the main goal of this work was to unravel the role of AQP4 under conditions of METH consumption...
March 10, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28279171/single-amino-acid-substitutions-in-the-selectivity-filter-render-nbxip1-1%C3%AE-aquaporin-water-permeable
#19
Henry Ampah-Korsah, Yonathan Sonntag, Angelica Engfors, Andreas Kirscht, Per Kjellbom, Urban Johanson
BACKGROUND: Aquaporins (AQPs) are integral membrane proteins that facilitate transport of water and/or other small neutral solutes across membranes in all forms of life. The X Intrinsic Proteins (XIPs) are the most recently recognized and the least characterized aquaporin subfamily in higher plants. XIP1s have been shown to be impermeable to water but permeable to boric acid, glycerol, hydrogen peroxide and urea. However, uncertainty regarding the determinants for selectivity and lack of an activity that is easy to quantify have hindered functional investigations...
March 9, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28265203/overexpression-of-aquaporin-4-in-articular-chondrocytes-exacerbates-the-severity-of-adjuvant-induced-arthritis-in-rats-an-in-vivo-and-in-vitro-study
#20
Li Cai, Chao Lei, Rong Li, Wei-Na Chen, Cheng-Mu Hu, Xiao-Yu Chen, Chun-Mei Li
BACKGROUND: The dysfunction of articular chondrocytes is a crucial step in rheumatoid arthritis (RA) pathogenesis while its molecular mechanisms are not fully known. This study was aimed to investigate the expression of aquaporin 4 (AQP4) in articular chondrocytes of adjuvant-induced arthritis (AIA) rats and its involvement in AIA development. METHODS: Thirty rats were divided into normal and AIA group (n = 15). Rat AIA was induced by intradermal injection of complete Freund's adjuvant and evaluated by secondary paw swelling and histological assessments on knee joint damage...
2017: Journal of Inflammation
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