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https://www.readbyqxmd.com/read/29045912/the-lived-experience-of-first-year-undergraduate-student-nurses-a-hermeneutic-phenomenological-study
#1
Debra J Porteous, Alison Machin
INTRODUCTION: This study gives insight into the experiences and perceptions of one group of undergraduate nursing students as they make the transition into Higher Education and the nursing profession, during the first year, of their three-year programme. Research has shown that first year undergraduate experience is complex and challenging for any student. For undergraduate nursing students, the process of achieving additional professional practice competencies required for United Kingdom nursing registration adds additional responsibility and potentially, more pressure...
October 3, 2017: Nurse Education Today
https://www.readbyqxmd.com/read/29045846/identification-of-genetically-intact-hiv-1-proviruses-in-specific-cd4-t-cells-from-effectively-treated-participants
#2
Bonnie Hiener, Bethany A Horsburgh, John-Sebastian Eden, Kirston Barton, Timothy E Schlub, Eunok Lee, Susanne von Stockenstrom, Lina Odevall, Jeffrey M Milush, Teri Liegler, Elizabeth Sinclair, Rebecca Hoh, Eli A Boritz, Daniel Douek, Rémi Fromentin, Nicolas Chomont, Steven G Deeks, Frederick M Hecht, Sarah Palmer
Latent replication-competent HIV-1 persists in individuals on long-term antiretroviral therapy (ART). We developed the Full-Length Individual Proviral Sequencing (FLIPS) assay to determine the distribution of latent replication-competent HIV-1 within memory CD4(+) T cell subsets in six individuals on long-term ART. FLIPS is an efficient, high-throughput assay that amplifies and sequences near full-length (∼9 kb) HIV-1 proviral genomes and determines potential replication competency through genetic characterization...
October 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/29045837/the-circadian-clock-gene-bmal1-controls-thyroid-hormone-mediated-spectral-identity-and-cone-photoreceptor-function
#3
Onkar B Sawant, Amanda M Horton, Olivia F Zucaro, Ricky Chan, Vera L Bonilha, Ivy S Samuels, Sujata Rao
Circadian clocks regulate various aspects of photoreceptor physiology, but their contribution to photoreceptor development and function is unclear. Cone photoreceptors are critical for color vision. Here, we define the molecular function of circadian activity within cone photoreceptors and reveal a role for the clock genes Bmal1 and Per2 in regulating cone spectral identity. ChIP analysis revealed that BMAL1 binds to the promoter region of the thyroid hormone (TH)-activating enzyme type 2 iodothyronine deiodinase (Dio2) and thus regulates the expression of Dio2...
October 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/29045826/a-cell-migration-tracking-tool-supports-coupling-of-tissue-rotation-to-elongation
#4
Dong-Yuan Chen, Justin Crest, David Bilder
Cell migration is indispensable to morphogenesis and homeostasis. Live imaging allows mechanistic insights, but long-term observation can alter normal biology, and tools to track movements in vivo without perturbation are lacking. We develop here a tool called M-TRAIL (matrix-labeling technique for real-time and inferred location), which reveals migration histories in fixed tissues. Using clones that overexpress GFP-tagged extracellular matrix (ECM) components, motility trajectories are mapped based on durable traces deposited onto basement membrane...
October 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/29045192/a-dna-vaccine-delivered-by-dermal-electroporation-fully-protects-cynomolgus-macaques-against-lassa-fever
#5
Kathleen A Cashman, Eric R Wilkinson, Carl I Shaia, Paul R Facemire, Todd M Bell, Jeremy J Bearss, Joshua D Shamblin, Suzanne E Wollen, Kate E Broderick, Niranjan Y Sardesai, Connie S Schmaljohn
Lassa virus (LASV) is an ambisense RNA virus in the Arenaviridae family and is the etiological agent of Lassa fever, a severe hemorrhagic disease endemic to West and Central Africa (1,2) . There are no U.S. Food and Drug Administration (FDA)-licensed vaccines available to prevent Lassa fever (1,2) . in our previous studies, we developed a gene-optimized DNA vaccine that encodes the glycoprotein precursor gene of LASV (Josiah strain) and demonstrated that three vaccinations accompanied by dermal electroporation protected guinea pigs from LASV-associated illness and death...
October 18, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29044963/using-sds-page-gel-finger-printing-to-identify-soft-bodied-wood-boring-insect-larvae-to-species
#6
Mark A O'Neill, Mia Denos, Daniel Reed
BACKGROUND: This paper describes the progress which we have made assessing the feasibility of "finger printing" using imaged SDS-PAGE gels of haemolymph proteins, to identify soft bodied wood boring insect larvae such as the Asian longhorn beetle, Anoplophora glabripennis (Motscholsky, 1853) (Coleoptera: Cerambycidae). Due to stringent import restrictions and difficulty in obtaining licences to work with these organisms, we opted to work with four species of scarab beetle, Mecynorhina polyphemus (Fabricius, 1781), Pachnoda sinuata (Fabricius ,1775), Eucidella shiratica (Csiki, 1909) and Eucidella shultzeorum (Kolbe, 1906) which have near identical larval morphologies...
October 17, 2017: Pest Management Science
https://www.readbyqxmd.com/read/29044430/molecular-regulation-of-sex-change-induced-by-methyltestosterone-feeding-and-methyltestosterone-feeding-withdrawal-in-the-protogynous-orange-spotted-grouper
#7
Qing Wang, Yun Liu, Cheng Peng, Xiang Wang, Ling Xiao, Dengdong Wang, Jiaxing Chen, Haifa Zhang, Huihong Zhao, Shuisheng Li, Yong Zhang, Haoran Lin
The sex identity of fish can be easily manipulated by exogenous hormones. Treatment with 17-methyltestosterone (MT) has been widely used to induce a male fate, but the molecular and cellular processes underlying sex changes induced by MT treatments and the withdrawal of MT are not well studied. In this study, we systematically investigated gonadal histology, gene expression profiles, sex steroid hormone levels, and cellular changes during sex changes induced by MT-feeding and MT-feeding withdrawal in the protogynous orange-spotted grouper, Epinephelus coioides...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29044428/expression-of-stra8-is-conserved-in-therian-mammals-but-expression-of-cyp26b1-differs-between-marsupials-and-mice
#8
Danielle E Hickford, Siew Fen Lisa Wong, Stephen R Frankenberg, Geoff Shaw, Hongshi Yu, Keng Yih Chew, Marilyn B Renfree
The first sign of mammalian germ cell sexual differentiation is the initiation of meiosis in females and of mitotic arrest in males. In the mouse, retinoic acid induces ovarian Stra8 expression and entry of germ cells into meiosis. In developing mouse testes, cytochrome P450 family 26, subfamily b, polypeptide 1 (CYP26B1) produced by the Sertoli cells degrades retinoic acid, preventing Stimulated by Retinoic Acid Gene 8 (Stra8), expression and inhibiting meiosis. However, in developing humans, there is no evidence that CYP26B1 acts a meiosis-inhibiting factor...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29043798/jozilebomines-a-and-b-naphthylisoquinoline-dimers-from-the-congolese-liana-ancistrocladus-ileboensis-with-antiausterity-activities-against-the-panc-1-human-pancreatic-cancer-cell-line
#9
Jun Li, Raina Seupel, Torsten Bruhn, Doris Feineis, Marcel Kaiser, Reto Brun, Virima Mudogo, Suresh Awale, Gerhard Bringmann
Two new naphthylisoquinoline dimers, jozilebomines A (1a) and B (1b), were isolated from the roots of the Congolese plant Ancistrocladus ileboensis, along with the known dimer jozimine A2 (2). These compounds are Dioncophyllaceae-type metabolites, i.e., lacking oxygen functions at C-6 and with an R-configuration at C-3 in their tetrahydroisoquinoline moieties. The dimers 1a and 1b consist of two 7,1'-coupled naphthylisoquinoline monomers linked through an unprecedented 3',6″-coupling in the binaphthalene core and not, as in 2, via the C-3-positions of the two naphthalene units...
October 18, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29042670/from-est-to-novel-spider-silk-gene-identification-for-production-of-spidroin-based-biomaterials
#10
Weidong Huang, Yan Zhang, Yifan Chen, Yin Wang, Wensu Yuan, Ning Zhang, Toong Jin Lam, Zhiyuan Gong, Daiwen Yang, Zhi Lin
A cDNA library from a pool of all the seven silk glands from a tropical spider species was constructed. More than 1000 expressed sequence tag (EST) clones were created. Almost 65% of the EST clones were identified and around 50% were annotated. The cellular and functional distribution of the EST clones indicated high protein synthesis activity in spider silk glands. Novel clones with repetitive amino acid sequences, which is one of the most important characteristics of spider silk genes, were isolated. One of these clones, namely TuSp2 in current research, contains two almost identical fragments with one short C-terminal domain...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042504/unintended-laboratory-driven-evolution-reveals-genetic-requirements-for-biofilm-formation-by-desulfovibrio-vulgaris-hildenborough
#11
Kara B De León, Grant M Zane, Valentine V Trotter, Gregory P Krantz, Adam P Arkin, Gareth P Butland, Peter J Walian, Matthew W Fields, Judy D Wall
Biofilms of sulfate-reducing bacteria (SRB) are of particular interest as members of this group are culprits in corrosion of industrial metal and concrete pipelines as well as being key players in subsurface metal cycling. Yet the mechanism of biofilm formation by these bacteria has not been determined. Here we show that two supposedly identical wild-type cultures of the SRB Desulfovibrio vulgaris Hildenborough maintained in different laboratories have diverged in biofilm formation. From genome resequencing and subsequent mutant analyses, we discovered that a single nucleotide change within DVU1017, the ABC transporter of a type I secretion system (T1SS), was sufficient to eliminate biofilm formation in D...
October 17, 2017: MBio
https://www.readbyqxmd.com/read/29042015/identification-of-monoclonal-antibodies-cross-reactive-with-bottlenose-dolphin-orthologues-of-the-major-histocompatibility-complex-and-leukocyte-differentiation-molecules
#12
Mahmoud M Elnaggar, Gaber S Abdellrazeq, Stephanie K Venn-Watson, Eric D Jensen, Victoria Hulubei, Lindsay M Fry, Randy E Sacco, William C Davis
The slow progress in understanding immunotoxic effects of environmental contaminants and their influence on disease susceptibility in whales is largely due to the limited information available on the immune systems and immune function of species included in the Cetancodontamorpha clade. Studies in species in the other major clades included in the Artiodactylamorpha, Ruminantiamorpha, Suinamorpha, and Camelidamorpha have revealed the immune systems are similar, but not identical. The present study was undertaken to expand the available monoclonal antibody reagents needed to gain insight into the composition, function, and evolution of the immune system in Cetancodontamorpha, using the dolphin (Tursiops truncatus) as a model cetacean species...
October 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29041987/the-effects-of-melatonin-on-colonization-of-neonate-spermatogonial-mouse-stem-cells-in-a-three-dimensional-soft-agar-culture-system
#13
Shadan Navid, Mehdi Abbasi, Yumi Hoshino
BACKGROUND: Melatonin is a pleiotropic hormone with powerful antioxidant activity both in vivo and in vitro. The present study aimed to investigate the effects of melatonin on the proliferation efficiency of neonatal mouse spermatogonial stem cells (SSCs) using a three-dimensional soft agar culture system (SACS) which has the capacity to induce development of SSCs similar to in vivo conditions. METHODS: SSCs were isolated from testes of neonate mice and their purities were assessed by flow cytometry using PLZF antibody...
October 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29041241/sub-picosecond-laser-damage-growth-on-high-reflective-coatings-for-high-power-applications
#14
Martin Sozet, Stéphane Bouillet, Johann Berthelot, Jérôme Neauport, Laurent Lamaignère, Laurent Gallais
Growth of laser damage on High Reflection (HR) thin film coatings is investigated at the wavelength of 1.030µm in the sub-picosecond regime. An experimental laser damage setup in a pump / probe configuration is used to study the growth behavior of engineered damage sites as well as laser damage sites. Results demonstrate that engineered sites and laser damage sites grow identically which indicates that the growth phenomenon is intrinsic to materials and stack design. In order to analyze the experimental results, we have developed a numerical model to simulate growth...
October 16, 2017: Optics Express
https://www.readbyqxmd.com/read/29041221/modal-noise-in-an-integrated-photonic-lantern-fed-diffraction-limited-spectrograph
#15
N Cvetojevic, N Jovanovic, S Gross, B Norris, I Spaleniak, C Schwab, M J Withford, M Ireland, P Tuthill, O Guyon, F Martinache, J S Lawrence
In an attempt to develop a streamlined astrophotonic instrument, we demonstrate the realization of an all-photonic device capable of both multimode to single mode conversion and spectral dispersion on an 8-m class telescope with efficient coupling. The device was a monolithic photonic spectrograph which combined an integrated photonic lantern and an efficient arrayed waveguide grating device. During on-sky testing, we discovered a previously unreported type of noise that made spectral extraction and calibration extremely difficult...
October 16, 2017: Optics Express
https://www.readbyqxmd.com/read/29040600/robust-inference-in-summary-data-mendelian-randomization-via-the-zero-modal-pleiotropy-assumption
#16
Fernando Pires Hartwig, George Davey Smith, Jack Bowden
Background: Mendelian randomization (MR) is being increasingly used to strengthen causal inference in observational studies. Availability of summary data of genetic associations for a variety of phenotypes from large genome-wide association studies (GWAS) allows straightforward application of MR using summary data methods, typically in a two-sample design. In addition to the conventional inverse variance weighting (IVW) method, recently developed summary data MR methods, such as the MR-Egger and weighted median approaches, allow a relaxation of the instrumental variable assumptions...
July 12, 2017: International Journal of Epidemiology
https://www.readbyqxmd.com/read/29040564/n-cadherin-identifies-human-endometrial-epithelial-progenitor-cells-by-in-vitro-stem-cell-assays
#17
Hong P T Nguyen, L Xiao, James A Deane, Ker-Sin Tan, Fiona L Cousins, Hirotaka Masuda, Carl N Sprung, Anna Rosamilia, Caroline E Gargett
STUDY QUESTION: Is there a specific surface marker that identifies human endometrial epithelial progenitor cells with adult stem cell activity using in vitro assays? SUMMARY ANSWER: N-cadherin isolates clonogenic, self-renewing human endometrial epithelial progenitor cells with high proliferative potential that differentiate into cytokeratin+ gland-like structures in vitro and identifies their location in some cells of gland profiles predominantly in basalis endometrium adjacent to the myometrium...
September 27, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29040061/validation-of-a-novel-biomechanical-test-bench-for-the-knee-joint-with-six-degrees-of-freedom
#18
Christian H Heinrichs, Dominik Knierzinger, Hannes Stofferin, Werner Schmoelz
A novel biomechanical test bench has been developed for in-vitro evaluation of the knee joint. The test bench allows the kinematics of the knee joint to be studied in all six degrees of freedom. Flexion-extension knee movements are induced by quadriceps and hamstring muscle forces simulated by five pneumatic cylinders. The kinematics of the knee and the actively applied muscle forces are measured simultaneously. The aim of this study was to validate the sensitivity and reproducibility of this novel test bench...
October 17, 2017: Biomedizinische Technik. Biomedical Engineering
https://www.readbyqxmd.com/read/29040012/outcome-of-acute-lymphoblastic-leukemia-in-children-with-down-syndrome-polish-pediatric-leukemia-and-lymphoma-study-group-report
#19
Joanna Zawitkowska, Teresa Odój, Katarzyna Drabko, Agnieszka Zaucha-Prażmo, Julia Rudnicka, Michał Romiszewski, Michał Matysiak, Kinga Kwiecińska, Magdalena Ćwiklińska, Walentyna Balwierz, Joanna Owoc-Lempach, Katarzyna Derwich, Jacek Wachowiak, Maciej Niedźwiecki, Elżbieta Adamkiewicz-Drożyńska, Joanna Trelińska, Wojciech Młynarski, Andrzej Kołtan, Mariusz Wysocki, Renata Tomaszewska, Tomasz Szczepański, Marcin Płonowski, Maryna Krawczuk-Rybak, Tomasz Ociepa, Tomasz Urasiński, Agnieszka Mizia-Malarz, Grażyna Sobol-Milejska, Grażyna Karolczyk, Jerzy Kowalczyk
Children with Down syndrome (DS) have a 20-fold increased risk of developing leukemia compared with the general population. The aim of the study was to analyze the outcome of patients diagnosed with Down syndrome and acute lymphoblastic leukemia (ALL) in Poland between the years 2003 and 2010. A total of 1848 children were diagnosed with ALL (810 females and 1038 males). Of those, 41 (2.2%) had DS. The children were classified into three risk groups: a standard-risk group-14 patients, an intermediate-risk group-24, a high-risk group-3...
October 17, 2017: Pediatric Hematology and Oncology
https://www.readbyqxmd.com/read/29039597/whole%C3%A2-genome-scale-identification-of-methylation-markers-specific-for-cerebral-palsy-in-monozygotic-discordant-twins
#20
Zhe Jiao, Zhimei Jiang, Jingtao Wang, Hui Xu, Qiang Zhang, Shuang Liu, Ning Du, Yuanyuan Zhang, Hongbin Qiu
Cerebral palsy (CP) is a severe type of brain disease affecting movement and posture. Although CP has strong genetic and environmental components, considerable differences in the methylome between monozygotic (MZ) twins discordant for CP implicates epigenetic contributors as well. In order to determine the differences in methylation in patients with CP without interference of the interindividual genomic variation, four pairs of MZ twins discordant for CP were profiled for DNA methylation changes using reduced representation bisulfite sequencing on the genomic‑scale...
October 17, 2017: Molecular Medicine Reports
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