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https://www.readbyqxmd.com/read/28633981/development-of-a-propidium-monoazide-polymerase-chain-reaction-assay-for-detection-of-viable-lactobacillus-brevis-in-beer
#1
Yanlin Ma, Yang Deng, Zhenbo Xu, Junyan Liu, Jianjun Dong, Hua Yin, Junhong Yu, Zongming Chang, Dongfeng Wang
The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. However, one of the drawbacks is the inability to differentiate between live and dead cells. In this paper, the combination of propidium monoazide (PMA) pretreatment and conventional PCR had been described. The established PMA-PCR identified beer spoilage Lactobacillus brevis based not on their identity, but on the presence of horA gene which we show to be highly correlated with the ability of beer spoilage LAB to grow in beer...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28633004/field-evaluations-of-newly-available-interference-free-monitors-for-nitrogen-dioxide-no2-and-ozone-o3-at-near-road-and-conventional-naaqs-compliance-sites
#2
Alan R Leston, Will M Ollison
Long standing measurement techniques for determining ground-level ozone (O3) and nitrogen dioxide (NO2) are known to be biased by interfering compounds that result in overestimates of high O3 and NO2 ambient concentrations under conducive conditions. An increasing near-ground O3 gradient (NGOG) with increasing height above ground level is also known to exist. Both the interference bias and NGOG were investigated by comparing data from a conventional Federal Equivalent Method (FEM) O3 photometer and an identical monitor upgraded with an "interference-free" nitric oxide O3 scrubber which alternatively sampled at 2 meter and 6...
June 20, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/28632401/motifsim-2-1-an-enhanced-software-platform-for-detecting-similarity-in-multiple-dna-motif-data-sets
#3
Ngoc Tam L Tran, Chun-Hsi Huang
Finding binding site motifs plays an important role in bioinformatics as it reveals the transcription factors that control the gene expression. The development for motif finders has flourished in the past years with many tools have been introduced to the research community. Although these tools possess exceptional features for detecting motifs, they report different results for an identical data set. Hence, using multiple tools is recommended because motifs reported by several tools are likely biologically significant...
June 20, 2017: Journal of Computational Biology: a Journal of Computational Molecular Cell Biology
https://www.readbyqxmd.com/read/28631554/the-effects-of-platelet-activating-factor-on-uterine-contractility-perfusion-hypoxia-and-pain-in-mice
#4
Kevin M Hellman, Peter Y Yu, Folabomi A Oladosu, Chaya Segel, Alice Han, Pottumarthi V Prasad, Tamas Jilling, Frank F Tu
It is widely hypothesized that menstrual pain is triggered by prostaglandin synthesis that evokes high-pressure uterine contractions and ischemia. However, the effects of molecules implicated in menstrual pain on uterine contractility, perfusion, and oxygenation in vivo have been rarely demonstrated. Studies in women that do not respond to nonsteroidal anti-inflammatory drugs (NSAIDs) have reported elevated levels of platelet-activating factor (PAF). To establish in vivo evidence of PAF's capability to impair uterine homeostasis and to elicit visceral pain, we examined the effects of the PAF receptor agonist (carbamyl PAF [CPAF]) in comparison to other molecules hypothesized to play a role in uterine pain in mice...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28631297/an-augmented-reality-tool-for-learning-spatial-anatomy-on-mobile-devices
#5
Nishant Jain, Patricia Youngblood, Matthew Hasel, Sakti Srivastava
INTRODUCTION: Augmented Realty (AR) offers a novel method of blending virtual and real anatomy for intuitive spatial learning. Our first aim in the study was to create a prototype AR tool for mobile devices. Our second aim was to complete a technical evaluation of our prototype AR tool focused on measuring the system's ability to accurately render digital content in the real world. MATERIALS AND METHODS: We imported Computed Tomography (CT) data derived virtual surface models into a 3D Unity engine environment and implemented an AR algorithm to display these on mobile devices...
June 20, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28631053/characterization-of-an-orf-virus-isolate-from-an-outbreak-in-heilongjiang-province-china
#6
Yongzhong Yu, Qiang Tan, Wenbo Zhao, Xue Zhang, Jinzhu Ma, Zhijun Wu, Zhanbo Zhu, Yudong Cui
Contagious ecthyma, caused by orf virus (ORFV), is an epitheliotrophic contagious disease with zoonotic implications that mainly affects sheep, goats, wild ruminants, and humans. Recently, a novel ORFV strain, OV/HLJ/04, was successfully isolated from the skin and mucosal lesions of a goat with severe clinical sore mouth symptoms in Heilongjiang province of China. The OV/HLJ/04 isolate was characterized by electron microscopy, serological tests, and experimental reproduction of disease. The purified virions exhibited a typical ovoid shape when observed by electron microscopy...
June 19, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28629960/molecular-cloning-and-mrna-expression-pattern-of-sox4-in-paramisgurnus-dabryanus
#7
Xiaohua Xia, Ruyan Wan, Weiran Huo, Linxia Zhang, Xiaopei Xia, Zhongjie Chang
Sox4 belonged to the SoxC subfamily of the Sox family, which play important roles in the development of the vertebrate gonad and nervous system. A Sox4 homologue was cloned from brain of Paramisgurnus dabryanus by using homologous cloning and rapid amplification of cDNA ends (RACE), designated as PdSox4. The full-length cDNA was 2163bp, containing the 759bp 5'-untranslated region, 267bp 3'-untranslated region and encoding a putative protein of 378 amino acids with a characteristic high mobility group box (HMG-box) DNA-binding domain of 79 amino acids with the specific motif (RPMNAFMVW)...
June 16, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28629449/delineating-the-genetic-heterogeneity-of-oca-in-hungarian-patients
#8
Beáta Fábos, Katalin Farkas, Lola Tóth, Adrienn Sulák, Kornélia Tripolszki, Mariann Tihanyi, Réka Németh, Krisztina Vas, Zsanett Csoma, Lajos Kemény, Márta Széll, Nikoletta Nagy
BACKGROUND: Oculocutaneous albinism (OCA) is a clinically and genetically heterogenic group of pigmentation abnormalities characterized by variable hair, skin, and ocular hypopigmentation. Six known genes and a locus on human chromosome 4q24 have been implicated in the etiology of isolated OCA forms (OCA 1-7). METHODS: The most frequent OCA types among Caucasians are OCA1, OCA2, and OCA4. We aimed to investigate genes responsible for the development of these OCA forms in Hungarian OCA patients (n = 13)...
June 19, 2017: European Journal of Medical Research
https://www.readbyqxmd.com/read/28629345/prevalence-and-incidence-of-overweight-and-obesity-among-vietnamese-preschool-children-a-longitudinal-cohort-study
#9
Loan Minh Do, Toan Khanh Tran, Bo Eriksson, Max Petzold, Henry Ascher
BACKGROUND: A plateau in childhood overweight and obesity has been reported in some developed countries while in almost all developing countries this problem is on the rise. The aim of this paper is to describe the changes in prevalence of overweight and obesity within a cohort of preschool children followed for 3 years, and to estimate and compare the incidences in urban and rural children of Hanoi, Vietnam. METHODS: A longitudinal study of a cohort of 2677 children aged 3 to 6 years old at the beginning of the study was conducted in urban DodaLab and rural FilaBavi, Hanoi, Vietnam...
June 19, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28627901/cone-of-gaze-in-positive-schizotypy-relationship-to-referential-thinking-and-social-functioning
#10
Heather M Wastler, Mark F Lenzenweger
Eye contact is an essential means of nonverbal communication, providing information about attention, emotion, mental state, facial expressions, and identity/gender (Itier & Batty, 2009). Although studies suggest that patients with schizophrenia endorse direct gaze more often than controls in ambiguous gaze circumstances, gaze perception in schizotypy remains unstudied. This study investigated whether individuals with positive schizotypic features incorrectly perceive that others are looking at them and whether this is related to referential thinking and psychosocial functioning...
June 19, 2017: Personality Disorders
https://www.readbyqxmd.com/read/28627467/time-to-review-the-gold-standard-for-genotyping-vancomycin-resistant-enterococci-in-epidemiology-comparing-whole-genome-sequencing-with-pfge-and-mlst-in-three-suspected-outbreaks-in-sweden-during-2013-2015
#11
Birgitta Lytsy, Lars Engstrand, Åke Gustafsson, Rene Kaden
Vancomycin-resistant enterococci (VRE) are a challenge to the health-care system regarding transmission rate and treatment of infections. VRE outbreaks have to be controlled from the first cases which means that appropriate and sensitive genotyping methods are needed. The aim of this study was to investigate the applicability of whole genome sequencing based analysis compared to Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST) in epidemiological investigations as well as the development of a user friendly method for daily laboratory use...
June 13, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28627251/direct-lc-ms-analysis-for-complete-characterization-of-recombinant-adeno-associated-virus-capsid-proteins
#12
Xiaoying Jin, Lin Liu, Shelley Nass, Catherine R O'Riordan, Eric Pastor, Kate Xiaokui Zhang
The requirement for robust analytical methods to support characterization of Adeno-Associated Virus (AAV) vectors is immediate, as the field advances more AAV gene therapeutics into the clinic and onto commercialization. AAV capsid proteins (VPs) are critical for viral infectivity and vector potency, thus, complete characterization of the constituent viral capsid proteins of AAV vector therapeutics, including their sequence and post-translational modifications (PTMs), is highly recommended to ensure AAV product quality and consistency...
June 18, 2017: Human Gene Therapy Methods
https://www.readbyqxmd.com/read/28626004/sex-chromosome-evolution-heterochiasmy-and-physiological-qtl-in-the-salmonid-brook-charr-salvelinus-fontinalis
#13
Ben J G Sutherland, Ciro Rico, Céline Audet, Louis Bernatchez
Whole genome duplication can have large impacts on genome evolution, and much remains unknown about these impacts. This includes the mechanisms of coping with a duplicated sex determination system and whether this has an impact on increasing the diversity of sex determination mechanisms. Other impacts include sexual conflict, where alleles having different optimums in each sex can result in sequestration of genes into non-recombining sex chromosomes. Sex chromosome development itself may involve sex-specific recombination rate (i...
June 16, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28625995/novel-antibiotic-resistance-determinants-from-agricultural-soil-exposed-to-antibiotics-widely-used-in-human-medicine-and-animal-farming
#14
Calvin Ho-Fung Lau, Kalene van Engelen, Stephen Gordon, Justin Renaud, Edward Topp
Antibiotic resistance has emerged globally as one of the biggest threats to human and animal health. Although the excessive use of antibiotics is recognized for accelerating the selection for resistance, there is a growing body of evidence suggesting that natural environments are "hotspots" for the development of both ancient and contemporary resistance mechanisms. Given that pharmaceuticals can be entrained onto agricultural land through anthropogenic activities, this could be a potential driver for the emergence and dissemination of resistance in soil bacteria...
June 16, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28625054/all-in-one-achieving-robust-strongly-luminescent-and-highly-dispersible-hybrid-materials-by-combining-ionic-and-coordinate-bonds-in-molecular-crystals
#15
Wei Liu, Kun Zhu, Simon J Teat, Gangotri Dey, Zeqing Shen, Lu Wang, Deirdre M O'Carroll, Jing Li
Extensive research has been pursued to develop low-cost and high-performance functional inorganic-organic hybrid materials for clean/renewable energy related applications. While great progress has been made in the recent years some key challenges remain to be tack-led. One major issue is the generally poor stability of these materials which originates from relatively fragile/weak bonds between inorganic and organic constituents. Herein, we report a unique "all-in-one" (AIO) approach in constructing robust structures with desired properties...
June 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28624470/molecular-cloning-and-expression-analysis-of-ccl25-and-its-receptor-ccr9s-from-epinephelus-coioides-post-cryptocaryon-irritans-infection
#16
Man Yang, Ling Zhou, Hai-Qing Wang, Xiao-Chun Luo, Xue-Ming Dan, Yan-Wei Li
Among other functions, CCL25/CCR9 has an important role in regulating the trafficking of developing T cells in the thymus, and in homing memory T cells to the small intestine. The function of this chemokine-receptor complex is not well studied in fish. We identified a CCL25-like (EcCCL25, 108 aa) and two CCR9-like sequences (EcCCR9aa 373 aa; and EcCCR9b, 375 aa) from a transcriptome database of orange-spotted grouper (Epinephelus coioides). EcCCL25, EcCCR9a, and EcCCR9b shared conserved structural features with homologs from mammals and from other fish, and a consistent relationship with phylogenetic trees and sequence identities...
June 14, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28624402/development-and-validation-of-a-simple-to-use-nomogram-for-predicting-5-10-and-15-year-survival-in-asymptomatic-adults-undergoing-coronary-artery-calcium-scoring
#17
Bríain Ó Hartaigh, Heidi Gransar, Tracy Callister, Leslee J Shaw, Joshua Schulman-Marcus, Wijnand J Stuijfzand, Valentina Valenti, Iksung Cho, Jackie Szymonifka, Fay Y Lin, Daniel S Berman, Hyuk-Jae Chang, James K Min
OBJECTIVES: The purpose of this study was to develop and validate a simple-to-use nomogram for prediction of 5-, 10-, and 15-year survival among asymptomatic adults. BACKGROUND: Simple-to-use prognostication tools that incorporate robust methods such as coronary artery calcium scoring (CACS) for predicting near-, intermediate- and long-term mortality are warranted. METHODS: In a consecutive series of 9,715 persons (mean age: 53.4 ± 10.5 years; 59...
June 9, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28623734/ig-seq-deep-sequencing-of-the-variable-region-of-atlantic-salmon-igm-heavy-chain-transcripts
#18
Aleksei Krasnov, Sven Martin Jørgensen, Sergey Afanasyev
Immunoglobulin M plays a key role in systemic protection of Atlantic salmon against pathogens. Until recent, studies have focused on antigen-specific antibodies and little is known about the IgM repertoire: its size, developmental changes and responses to antigens. We report the development of deep sequencing protocol to characterize the repertoire of IgM heavy chain variable region. Its structure and changes were examined at the early stages of life and after infection with virus of cardiac myopathy. Clonotypes are identified by the V and J gene segments and amino acid sequences of CDR3, which determine the contribution of the heavy chain to the antigen binding properties...
June 14, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28623326/sialic-acid-linkage-differentiation-of-glycopeptides-using-capillary-electrophoresis-electrospray-ionization-mass-spectrometry
#19
Guinevere S M Kammeijer, Bas C Jansen, Isabelle Kohler, Anthonius A M Heemskerk, Oleg A Mayboroda, Paul J Hensbergen, Julie Schappler, Manfred Wuhrer
Sialylation is a glycosylation feature that occurs in different linkages at the non-reducing end of a glycan moiety, the linkage isomers are often differentially associated with various biological processes. Due to very similar physico-chemical properties, the separation of isomeric sialylated glycopeptides remains challenging but of utmost importance in the biomedicine and biotechnology, including biomarker discovery, glyco-engineering and biopharmaceutical characterization. This study presents the implementation of a high-resolution separation platform based on capillary electrophoresis - mass spectrometry (CE-MS) allowing for the selective analysis of α2,3- and α2,6-sialylated glycopeptides...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28623052/how-physicians-draw-satisfaction-and-overcome-barriers-in-their-practices-it-sustains-me
#20
William T Branch, Amy B Weil, MaryAnn C Gilligan, Debra K Litzelman, Janet P Hafler, Margaret Plews-Ogan, Elizabeth A Rider, Lars G Osterberg, Dana Dunne, Arthur R Derse, J Richard Pittman, Richard M Frankel
OBJECTIVE: Major reorganizations of medical practice today challenge physicians' ability to deliver compassionate care. We sought to understand how physicians who completed an intensive faculty development program in medical humanism sustain their humanistic practices. METHODS: Program completers from 8 U.S. medical schools wrote reflections in answer to two open-ended questions addressing their personal motivations and the barriers that impeded their humanistic practice and teaching...
June 8, 2017: Patient Education and Counseling
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