keyword
MENU ▼
Read by QxMD icon Read
search

Separ

keyword
https://www.readbyqxmd.com/read/28432941/validation-of-a-spe-hplc-uv-method-for-the-quantification-of-a-new-er-specific-photosensitizer-or-141-in-blood-serum-using-total-error-concept
#1
Emilie Bony, Yohan Mace, Adán Pinto, David Delvaux, Robert Kiss, Olivier Riant, Olivier Feron, Joëlle Quetin-Leclercq
The aim of this work is to develop the first validated HPLC-UV method quantification in blood serum for a new endoplasmic reticulum (ER)-specific benzophenazine photosensitizer (OR-141). A fast solid phase extraction (SPE) cleaning sample procedure was achieved on C18 encapped (ec) SPE cartridges and the separation was performed on a RP-18e column (5μM) using an isocratic elution with methanol. The method has been fully validated according to accuracy profiles based on total error and tolerance intervals. Calibration was performed in the matrix and trueness (<4...
April 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28432930/an-innovative-procedure-for-norm-scales-treatment-and-radionuclides-separation
#2
Jamal Al Abdullah, Mohammad Said Al-Masri, Yusr Amin, Hussam Khalily, Mohammad Ammar, Samer Nassour
The present study focuses on the development of a new chemical treatment method for naturally occurring radioactive materials (NORM) scale wastes from the oil industry. The method consists of three-stages, including the separation of oil using the Fenton oxidation process at room temperature, the complete dissolution of soluble and slightly soluble salts and separation of Ra and Pb isotopes. The proposed method can be considered as an innovative procedure for NORM scales treatment and radionuclides separation, which in turn support the radioactive waste management with economic benefits...
April 5, 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/28432915/proton-induced-production-and-radiochemical-isolation-of-44-ti-from-scandium-metal-targets-for-44-ti-44-sc-generator-development
#3
Valery Radchenko, Jonathan W Engle, Dmitri G Medvedev, Joel M Maassen, Cleo M Naranjo, George A Unc, Catherine A L Meyer, Tara Mastren, Mark Brugh, Leonard Mausner, Cathy S Cutler, Eva R Birnbaum, Kevin D John, F Meiring Nortier, Michael E Fassbender
Scandium-44g (half-life 3.97h) shows promise for application in positron emission tomography (PET), due to favorable decay parameters. One of the sources of (44g)Sc is the (44)Ti/(44g)Sc generator, which can conveniently provide this radioisotope on a daily basis at a diagnostic facility. Titanium-44 (half-life 60.0 a), in turn, can be obtained via proton irradiation of scandium metal targets. A substantial (44)Ti product batch, however, requires high beam currents, long irradiation times and an elaborate chemical procedure for (44)Ti isolation and purification...
April 7, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/28432905/statistical-analysis-of-dislocations-and-dislocation-boundaries-from-ebsd-data
#4
C Moussa, M Bernacki, R Besnard, N Bozzolo
Electron BackScatter Diffraction (EBSD) is often used for semi-quantitative analysis of dislocations in metals. In general, disorientation is used to assess Geometrically Necessary Dislocations (GNDs) densities. In the present paper, we demonstrate that the use of disorientation can lead to inaccurate results. For example, using the disorientation leads to different GND density in recrystallized grains which cannot be physically justified. The use of disorientation gradients allows accounting for measurement noise and leads to more accurate results...
April 14, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28432890/characterization-of-spectral-responses-of-dissolved-organic-matter-dom-for-atrazine-binding-during-the-sorption-process-onto-black-soil
#5
Yifan Wang, Xinyuan Zhang, Xing Zhang, Qingjuan Meng, Fengjie Gao, Ying Zhang
This study was aim to investigate the interaction between soil-derived dissolved organic matter (DOM) and atrazine as a kind of pesticides during the sorption process onto black soil. According to the experimental data, the adsorption capacity of Soil + DOM, Soil and DOM were 41.80, 31.45 and 9.35 mg kg(-1), separately, which indicated that DOM significantly enhanced the adsorption efficiency of atrazine by soil. Data implied that the pseudo-second-order kinetic equation could well explain the adsorption process...
April 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28432876/effect-of-pistachio-consumption-on-the-modulation-of-urinary-gut-microbiota-related-metabolites-in-prediabetic-subjects
#6
Pablo Hernández-Alonso, Daniel Cañueto, Simona Giardina, Jordi Salas-Salvadó, Nicolau Cañellas, Xavier Correig, Mònica Bulló
The specific nutritional composition of nuts could affect different metabolic pathways involved in a broad range of metabolic diseases. We therefore investigated whether chronic consumption of pistachio nuts modifies the urine metabolome in prediabetic subjects. We designed a randomized crossover clinical trial in 39 prediabetic subjects. They consumed a pistachio-supplemented diet (PD, 50% carbohydrates, 33% fat, including 57 g/d of pistachios daily) and a control diet (CD, 55% carbohydrates, 30% fat) for 4 months each, separated by a 2-week wash-out...
April 12, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28432819/identification-of-bioactive-compounds-in-shaoyao-gancao-decoction-using-%C3%AE-2-adrenoceptor-affinity-chromatography
#7
Zehua Li, Haiyang Gao, Jiangying Li, Yajun Zhang
Shaoyao-Gancao decoction, a Chinese herbal formula, is composed of Paeoniae Radix alba and Glycyrrhiza Radix et rhizoma. It has been widely used to treat muscle spasms and asthma. However, little is known about the bioactive components of Shaoyao-Gancao decoction. In the present study, the bioactive compounds in water-extract of Shaoyao-Gancao decoction were separated by the immobilized β2 -adrenoceptor affinity column and identified using quadrupole time-of-flight mass spectrometry. The affinity constants of the separated compounds that bound to β2 -adrenoceptor were determined by frontal analysis...
April 22, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28432795/validation-of-sun-exposure-reported-annually-against-interim-self-report-and-daily-sun-diaries
#8
Laura King, Fan Xiang, Ashwin Swaminathan, Keith Dear, Simone L Harrison, Ingrid van der Mei, Michael G Kimlin, Catherine D'Este, Robyn M Lucas
Data on personal sun exposure over a period exceeding the immediate past days or weeks is typically self-reported in brief questionnaire items. The validity of such self-reporting of longer-term personal sun exposure, e.g. over a year, including detail on variation across seasons, has not previously been investigated. In a volunteer sample (n = 331) of Australian adults age 18 years and over, we assessed the 12-month reliability of sun exposure reported separately for each season, and its accuracy compared to a daily sun diary in the same season...
April 22, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28432793/single-and-dual-cyclodextrins-systems-for-the-enantiomeric-and-diastereoisomeric-separations-of-structurally-related-dihydropyridone-analogues
#9
Thibaut Delplanques, Rajaa Boulahjar, Julie Charton, Corentin Houze, Michaël Howsam, Anne-Catherine Servais, Marianne Fillet, Emmanuelle Lipka
Cyclodextrin capillary electrophoresis methods (CD-CZE) were developed for complete enantiomeric and diastereoisomeric separations of a series of ten dihydropyridone analogues, of which eight were neutral, one was anionic and one was cationic. Ten different systems comprising one or two cyclodextrins were found to successfully separate the isomers thanks to a screening approach. Among the tested cyclodextrins, HS-γ-CD, either in a single or in a dual system, in a phosphate buffer using capillaries dynamically coated with polyethylene oxide (PEO), and SBE-β-CD, either in a single or in a dual system, in a borate buffer using uncoated capillaries, were the most selective selectors...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28432777/preparative-isoelectric-focusing-in-a-cellulose-based-separation-medium
#10
Jiří Šalplachta, Marie Horká, Karel Šlais
An improved preparative method based on isoelectric focusing of analytes in a cellulose-based separation medium is described in this study. Cellulose is suspended in an aqueous solution of simple buffers, ethylene glycol, glycerol, nonionic surfactant, and colored pI markers. Water partially evaporates during focusing run and the separation takes place in an in-situ generated layer of cellulose, which has a gel-like appearance at the end of analysis. Final positions of analytes are indicated by the positions of zones of focused pI markers...
April 22, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28432770/chiral-separation-of-aryloxyphenoxy-propionate-herbicides-in-a-permethyl-%C3%AE-cyclodextrin-based-column-influence-of-temperature-and-mobile-phase-composition-on-enantioselectivity
#11
Ester Lubomirsky, Héctor Di Loreto, Juan M Padró, Cecilia B Castells
We used a permethyl-β-cyclodextrin chiral stationary phase under reversed-phase conditions for the chiral separation of four aryloxyphenoxy-propionate herbicides (fenoxaprop-p-ethyl, quizalofop-p-ethyl and tefuryl and haloxyfop-p-methyl) with mixtures of methanol, ethanol, 2-propanol, n-propanol, tert-butanol, or acetonitrile and water as mobile phases and investigated the influence of mobile phase composition and column temperature (from 0 to 50 °C) on the separation. The retention factors (k) and selectivity factors (α) of all the herbicides investigated decreased with increasing temperature...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28432747/whole-body-adipose-tissue-and-lean-muscle-volumes-and-their-distribution-across-gender-and-age-mr-derived-normative-values-in-a-normal-weight-swiss-population
#12
Erika J Ulbrich, Daniel Nanz, Olof Dahlqvist Leinhard, Magda Marcon, Michael A Fischer
PURPOSE: To determine age- and gender-dependent whole-body adipose tissue and muscle volumes in healthy Swiss volunteers in Dixon MRI in comparison with anthropometric and bioelectrical impedance (BIA) measurements. METHODS: Fat-water-separated whole-body 3 Tesla MRI of 80 healthy volunteers (ages 20 to 62 years) with a body mass index (BMI) of 17.5 to 26.2 kg/m(2) (10 men, 10 women per decade). Age and gender-dependent volumes of total adipose tissue (TAT), visceral adipose tissue (VAT), total abdominal subcutaneous adipose tissue (ASAT) and total abdominal adipose tissue (TAAT), and the total lean muscle tissue (TLMT) normalized for body height were determined by semi-automatic segmentation, and correlated with anthropometric and BIA measurements as well as lifestyle parameters...
April 22, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28432695/recent-strategies-towards-microfluidic-based-surface-enhanced-raman-spectroscopy
#13
REVIEW
Anna Tycova, Jan Prikryl, Frantisek Foret
Surface enhanced Raman spectroscopy (SERS) is an extremely powerful analytical tool, which not only yields information about the molecular structure of the analyte in the form of characteristic vibrational spectrum but also gives sensitivities approaching those in fluorescence spectroscopy. The SERS measurement on the microfluidic platform provides possibility to manufacture the device with design perfectly fulfilling the needs of the application with minimal sample consumption. This review aims at describing basic strategies for SERS measurement in microfluidic devices published in the last decade and covers current trends in microfluidics with SERS detection in the field of bioanalysis and approaches towards on-line coupling of liquid-based separation techniques with SERS detection...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28432685/chiral-analysis-of-%C3%AE-diimine-ru-ii-and-fe-ii-complexes-by-capillary-electrophoresis-using-sulfated-cyclodextrins-as-stereoselectors
#14
Petra Sázelová, Dušan Koval, Lukáš Severa, Filip Teplý, Václav Kašička
Capillary electrophoresis (CE) using randomly highly sulfated α-, β-, and γ-cyclodextrins (S-α-CD, S-β-CD, S-γ-CD), sulfobutylether-β-CD (SBE-β-CD), single isomer (6-O-sulfo) α-, β-, and γ-CDs and their derivatives as stereoselectors was applied to chiral analysis of polypyridyl complexes of [Ru(bpy)3 ](2+) , [Ru(phen)3 ](2+) , and [Fe(phen)3 ](2+) (bpy = 2,2'-bipyridine; phen = 1,10 phenanthroline). The best separations of Δ- and Λ-enantiomers of the these complexes with high resolution (up to R1,2  = 7...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28432684/characterization-of-interactions-of-metal-containing-nanoparticles-with-biomolecules-by-ce-an-update-2012-2016
#15
REVIEW
Svetlana S Aleksenko, Magdalena Matczuk, Andrei R Timerbaev
The methodological developments and applications of CE related to studying biotransformations of metal-based nanoscale particles of impending medicinal use are overviewed. This is an update to a previous review article (Aleksenko, S. S., Shmykov, A. Y., Oszwałdowski, S., Timerbaev, A. R., Metallomics 2012, 4, 1141-1148) and it covers the research papers published within the last five years. As was anticipated in that review, CE can now be seen as a customary technique in the analysis of biomolecular interactions that exert an impact on the mechanism of action of nanoparticles, comprising metabolism, delivery, cell processing, and targeting...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28432653/analyzing-glycopeptide-isomers-by-combining-differential-mobility-spectrometry-with-electron-and-collision-based-tandem-mass-spectrometry
#16
J Larry Campbell, Takashi Baba, Chang Liu, Catherine S Lane, J C Yves Le Blanc, James W Hager
Differential mobility spectrometry (DMS) has been employed to separate isomeric species in several studies. Under the right conditions, factors such as separation voltage, temperature, the presence of chemical modifiers, and residence time can combine to provide unique signal channels for isomeric species. In this study, we examined a set of glycopeptide isomers, MUC5AC-3 and MUC5AC-13, which bear an N-acetyl-galactosamine (GalNAc) group on either threonine-3 or threonine-13. When analyzed as a mixture, the resulting MS and MS/MS spectra yield fragmentation patterns that cannot discern these convolved species...
April 21, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28432618/the-identification-of-key-genes-and-pathways-in-hepatocellular-carcinoma-by-bioinformatics-analysis-of-high-throughput-data
#17
Chaoyang Zhang, Li Peng, Yaqin Zhang, Zhaoyang Liu, Wenling Li, Shilian Chen, Guancheng Li
Liver cancer is a serious threat to public health and has fairly complicated pathogenesis. Therefore, the identification of key genes and pathways is of much importance for clarifying molecular mechanism of hepatocellular carcinoma (HCC) initiation and progression. HCC-associated gene expression dataset was downloaded from Gene Expression Omnibus database. Statistical software R was used for significance analysis of differentially expressed genes (DEGs) between liver cancer samples and normal samples. Gene Ontology (GO) term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, based on R software, were applied for the identification of pathways in which DEGs significantly enriched...
June 2017: Medical Oncology
https://www.readbyqxmd.com/read/28432614/dual-activity-of-hydroxypropyl-%C3%AE-cyclodextrin-and-water-soluble-carriers-on-the-solubility-of-carvedilol
#18
Abdelmoumin Zoghbi, Tianjiao Geng, Bo Wang
Carvedilol (CAR) is a non-selective α and β blocker categorized as class II drug with low water solubility. Several recent studies have investigated ways to overcome this problem. The aim of the present study was to combine two of these methods: the inclusion complex using hydroxypropyl-β-cyclodextrin (HPβCD) with solid dispersion using two carriers: Poloxamer 188 (PLX) and Polyvinylpyrrolidone K-30 (PVP) to enhance the solubility, bioavailability, and the stability of CAR. Kneading method was used to prepare CAR-HPβCD inclusion complex (KD)...
April 21, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28432613/pediatric-tonsillectomy-is-a-resource-intensive-procedure-a-study-of-canadian-health-administrative-data
#19
Kimmo T T Murto, Sherri L Katz, Daniel I McIsaac, Matthew A Bromwich, Régis Vaillancourt, Carl van Walraven
BACKGROUND: The majority of pediatric surgeries are performed in a day surgery setting. The rate of adverse postoperative outcomes and the factors that influence them are poorly described in the Canadian setting. Concerns about the safety of adenotonsillectomy (AT) have been raised. The objective of this Ontario-based study was to determine the rates and risks of hospital readmission, emergency department (ED) visits, or deaths within 30 days following common pediatric surgeries, with an emphasis on AT...
April 21, 2017: Canadian Journal of Anaesthesia, Journal Canadien D'anesthésie
https://www.readbyqxmd.com/read/28432550/estradiol-mitigates-ischemia-reperfusion-induced-acute-renal-failure-through-nmda-receptor-antagonism-in-rats
#20
Amrit Pal Singh, Nirmal Singh, Preet Mohinder Singh Bedi
In the present study, we investigated possible involvement of N-methyl-D-aspartate receptors (NMDAR) in estradiol mediated protection against ischemia reperfusion (I/R)-induced acute renal failure (ARF) in rats. Bilateral renal ischemia of 40 min followed by reperfusion for 24 h induced ARF in male wistar rats. Quantification of serum creatinine, creatinine clearance (CrCl), blood urea nitrogen (BUN), uric acid, potassium, fractional excretion of sodium (FeNa), and urinary microproteins was done to assess I/R-induced renal damage in rats...
April 21, 2017: Molecular and Cellular Biochemistry
keyword
keyword
74535
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"