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https://www.readbyqxmd.com/read/28337556/homologous-gene-targeting-of-a-carotenoids-biosynthetic-gene-in-rhodosporidium-toruloides-by-agrobacterium-mediated-transformation
#1
Wenyi Sun, Xiaobing Yang, Xueying Wang, Xinping Lin, Yanan Wang, Sufang Zhang, Yushi Luan, Zongbao K Zhao
OBJECTIVES: To target a carotenoid biosynthetic gene in the oleaginous yeast Rhodosporidium toruloides by using the Agrobacterium-mediated transformation (AMT) method. RESULTS: The RHTO_04602 locus of R. toruloides NP11, previously assigned to code the carotenoid biosynthetic gene CRTI, was amplified from genomic DNA and cloned into the binary plasmid pZPK-mcs, resulting in pZPK-CRT. A HYG-expression cassette was inserted into the CRTI sequence of pZPK-CRT by utilizing the restriction-free clone strategy...
March 23, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28303649/transforming-sugars-into-fat-lipid-biosynthesis-using-different-sugars-in-yarrowia-lipolytica
#2
Piotr Hapeta, Magdalena Rakicka, Remi Dulermo, Heber Gamboa-Meléndez, Anne-Marie Crutz-Le Coq, Jean-Marc Nicaud, Zbigniew Lazar
In an era of ever-increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as Yarrowia lipolytica to convert waste materials into biofuels. Here, we constructed two Y. lipolytica strains that displayed both increased lipid accumulation and a more efficient use of biomass-derived sugars, including glucose, fructose, galactose, and inulin. The first strain, Y. lipolytica YLZ150, was derived from the French wild-type strain W29. It had inhibited triacylglycerol mobilization (∆tgl4) and β-oxidation (∆pox1-6), and it overexpressed GPD1, DGA2, HXK1, the native Leloir pathway, SUC2 from Saccharomyces cerevisiae, and INU1 from Kluyveromyces marxianus...
March 17, 2017: Yeast
https://www.readbyqxmd.com/read/28302588/nuclear-transformation-and-functional-gene-expression-in-the-oleaginous-microalga-monoraphidium-neglectum
#3
Daniel Jaeger, Wolfgang Hübner, Thomas Huser, Jan H Mussgnug, Olaf Kruse
Photosynthetic microalgae hold great promise as non-food feedstocks for the sustainable production of a range of bio-products and genetic engineering is an increasingly important strategy to improve the natural cellular features. For the vast majority of microalgal strains, however, genetic engineering is not yet possible and the establishment of efficient genetic tools for a broad range of microalgal species is an important task to reach biotechnological success. Stable DNA transformation is one of the crucial steps for most genetic engineering approaches...
March 13, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28295166/engineering-oxidative-stress-defense-pathways-to-build-a-robust-lipid-production-platform-in-yarrowia-lipolytica
#4
Peng Xu, Kangjian Qiao, Gregory Stephanopoulos
Microbially-derived lipids have recently attracted renewed interests due to their broad applications in production of green diesels, cosmetic additives and oleochemicals. Metabolic engineering efforts have targeted a large portfolio of biosynthetic pathways to efficiently convert sugar to lipids in oleaginous yeast. In the engineered overproducing strains, endogenous cell metabolism typically generates harmful electrophilic molecules that compromise cell fitness and productivity. Lipids, particularly unsaturated fatty acids, are highly susceptible to oxygen radical attack and the resulting oxidative species are detrimental to cell metabolism and limit lipid productivity...
March 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28270203/the-effects-of-torc-signal-interference-on-lipogenesis-in-the-oleaginous-yeast-trichosporon-oleaginosus
#5
Felix Bracharz, Veronika Redai, Kathrin Bach, Farah Qoura, Thomas Brück
BACKGROUND: Oleaginous organisms are a promising, renewable source of single cell oil. Lipid accumulation is mainly induced by limitation of nutrients such as nitrogen, phosphorus or sulfur. The oleaginous yeast Trichosporon oleaginosus accumulates up to 70% w/w lipid under nitrogen stress, while cultivation in non-limiting media only yields 9% w/w lipid. Uncoupling growth from lipid accumulation is key for the industrial process applicability of oleaginous yeasts. This study evaluates the effects of rapamycin on TOR specific signaling pathways associated with lipogenesis in Trichosporon oleaginosus for the first time...
March 7, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28266581/the-role-of-lipid-droplets-in-mortierella-alpina-aging-revealed-by-integrative-subcellular-and-whole-cell-proteome-analysis
#6
Yadong Yu, Tao Li, Na Wu, Ling Jiang, Xiaojun Ji, He Huang
Lipid droplets (LDs) participate in many cellular processes in oleaginous microorganisms. However, the exact function of LDs in the Mortierella alpina aging process remains elusive. Herein, subcellular proteomics was employed to unveil the composition and dynamics of the LD proteome in the aging M. alpina for the first time. More than 400 proteins were detected in LDs and 62 of them changed expression significantly during aging. By combining the LD proteomic data with whole-cell data, we found that the carbohydrate metabolism and de novo lipid biosynthesis were all inhibited during aging of M...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28258805/recent-advances-and-industrial-viewpoint-for-biological-treatment-of-wastewaters-by-oleaginous-microorganisms
#7
REVIEW
Chao Huang, Mu-Tan Luo, Xue-Fang Chen, Lian Xiong, Xiao-Mei Li, Xin-De Chen
Recently, technology of using oleaginous microorganisms for biological treatment of wastewaters has become one hot topic in biochemical and environmental engineering for its advantages such as easy for operation in basic bioreactor, having potential to produce valuable bio-products, efficient wastewaters treatment in short period, etc. To promote its industrialization, this article provides some comprehensive analysis of this technology such as its advances, issues, and outlook especially from industrial viewpoint...
February 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28231637/preen-oil-and-bird-fitness-a-critical-review-of-the-evidence
#8
Gregorio Moreno-Rueda
The uropygial gland is a holocrine complex exclusive to birds that produces an oleaginous secretion (preen oil) whose function is still debated. Herein, I examine critically the evidence for the many hypotheses of potential functions of this gland. The main conclusion is that our understanding of this gland is still in its infancy. Even for functions that are considered valid by most researchers, real evidence is scarce. Although it seems clear that preen oil contributes to plumage maintenance, we do not know whether this is due to a role in reducing mechanical abrasion or in reducing feather degradation by keratinophilic organisms...
February 23, 2017: Biological Reviews of the Cambridge Philosophical Society
https://www.readbyqxmd.com/read/28228091/exploring-the-transcriptome-of-non-model-oleaginous-microalga-dunaliella-tertiolecta-through-high-throughput-sequencing-and-high-performance-computing
#9
Lina Yao, Kenneth Wei Min Tan, Tin Wee Tan, Yuan Kun Lee
BACKGROUND: RNA-Seq technology has received a lot of attention in recent years for microalgal global transcriptomic profiling. It is widely used in transcriptome-wide analysis of gene expression., particularly for microalgal strains with potential as biofuel sources. However, insufficient genomic or transcriptomic information of non-model microalgae has limited the understanding of their regulatory mechanisms and hampered genetic manipulation to enhance biofuel production. As such, an optimal microalgal transcriptomic database construction is a subject of urgent investigation...
February 22, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28220353/exploitation-of-genus-rhodosporidium-for-microbial-lipid-production
#10
REVIEW
Jingyang Xu, Dehua Liu
Oleaginous microorganisms are receiving significant attention worldwide for their utility in biodiesel production and the potentiality to produce some specialty-type lipids. There is an increasing interest in isolation/adaption of robust microbe strains and design of innovative fermentation processes to make microbial lipid production a more efficient and economically feasible bio-process. Currently, the genus Rhodosporidium has been considered an important candidate, for the reason that several strains belonging to this genus have shown excellent capabilities of lipid accumulation, broad adaptabilities to various substrates, and co-production of some carotenoids...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28220186/efficient-gene-targeting-in-non-homologous-end-joining-deficient-lipomyces-starkeyi-strains
#11
Yoshifumi Oguro, Harutake Yamazaki, Satoshi Ara, Yosuke Shida, Wataru Ogasawara, Masamichi Takagi, Hiroaki Takaku
Microbial lipids are sustainable feedstock for the production of oleochemicals and biodiesel. Oleaginous yeasts have recently been proposed as alternative lipid producers to plants and animals to promote sustainability in the chemical and fuel industries. The oleaginous yeast Lipomyces starkeyi has great industrial potential as an excellent lipid producer. However, improvement of its lipid productivity is essential for the cost-effective production of oleochemicals and fuels. Genetic and metabolic engineering of L...
February 20, 2017: Current Genetics
https://www.readbyqxmd.com/read/28218992/differently-localized-lysophosphatidic-acid-acyltransferases-crucial-for-triacylglycerol-biosynthesis-in-the-oleaginous-alga-nannochloropsis
#12
Takashi Nobusawa, Koichi Hori, Hiroshi Mori, Ken Kurokawa, Hiroyuki Ohta
Production of renewable bioenergy will be necessary to meet rising global fossil fuel demands. Members of the marine microalgae genus Nannochloropsis produce large amounts of oils (triacylglycerols; TAGs), and this genus is regarded as one of the most promising for biodiesel production. Recent genome sequencing and transcriptomic studies on Nannochloropsis have provided a foundation for understanding its oleaginous trait, but the mechanism underlying oil accumulation remains to be clarified. Here we report Nannochloropsis knockout strains of four extraplastidic lysophosphatidic acid acyltransferases (LPAT1-4), which catalyze a major de novo biosynthetic step of TAGs and membrane lipids...
February 20, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28217743/regulation-of-nitrogen-metabolism-by-gata-zinc-finger-transcription-factors-in-yarrowia-lipolytica
#13
Kyle R Pomraning, Erin L Bredeweg, Scott E Baker
Fungi accumulate lipids in a manner dependent on the quantity and quality of the nitrogen source on which they are growing. In the oleaginous yeast Yarrowia lipolytica, growth on a complex source of nitrogen enables rapid growth and limited accumulation of neutral lipids, while growth on a simple nitrogen source promotes lipid accumulation in large lipid droplets. Here we examined the roles of nitrogen catabolite repression and its regulation by GATA zinc finger transcription factors on lipid metabolism in Y...
January 2017: MSphere
https://www.readbyqxmd.com/read/28213136/systematic-development-of-a-two-stage-fed-batch-process-for-lipid-accumulation-in-rhodotorula-glutinis
#14
Eric Lorenz, Dennis Runge, Anna-Maria Marbà-Ardébol, Maximilian Schmacht, Ulf Stahl, Martin Senz
The application of oleaginous yeast cells as feed supplement, for instance in aqua culture, can be a meaningful alternative for fish meal and oil additives. Therefore, a two-stage fed-batch process split into growth and lipogenesis phase was systematically developed to enrich the oleaginous yeast Rhodotorula glutinis Rh-00301 with high amounts of lipids at industrial relevant biomasses. Thereby, the different carbon sources glucose, sucrose and glycerol were investigated concerning their abilities to serve as a suited raw material for growth and/or lipid accumulation...
March 20, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28194497/erratum-to-development-of-a-sufficient-and-effective-procedure-for-transformation-of-an-oleaginous-yeast-rhodosporidium-toruloides-dmku3-tk16
#15
Yung-Yu Tsai, Takao Ohashi, Takenori Kanazawa, Pirapan Polburee, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama
No abstract text is available yet for this article.
February 13, 2017: Current Genetics
https://www.readbyqxmd.com/read/28188651/the-torc1-sch9-rim15-signaling-pathway-represses-yeast-to-hypha-transition-in-response-to-glycerol-availability-in-the-oleaginous-yeast-yarrowia-lipolytica
#16
Shu-Heng Liang, Heng Wu, Rui-Rui Wang, Qiang Wang, Tao Shu, Xiang-Dong Gao
The yeast-to-hypha dimorphic transition is important for survival under nutrient starvation in fungi. The oleaginous yeast Yarrowia lipolytica grows in the oval-shaped yeast form in glycerol media whereas it adopts a filamentous form in glucose media. It is not clear why this yeast responds differently to glycerol and glucose. Here, we show that glycerol blocks dimorphic transition even in the presence of glucose whereas glycerol depletion induces filamentous growth, suggesting that dimorphic transition is repressed in response to glycerol availability...
February 11, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28188644/rnai-based-targeted-gene-knockdown-in-the-model-oleaginous-microalgae-nannochloropsis-oceanica
#17
Li Wei, Yi Xin, Qintao Wang, Juan Yang, Hanhua Hu, Jian Xu
Microalgae are promising feedstock for renewable fuels such as biodiesel, yet development of industrial oleaginous strains has been hindered by the paucity and inefficiency of reverse genetics tools. Here we established an efficient RNAi-based targeted gene-knockdown method for Nannochloropsis spp., which are emerging model organisms for industrial microalgal oil production. The method achieved a 40-80% success rate in Nannochloropsis oceanica strain IMET1. When transcript level of one carbonic anhydrase (CA) was inhibited by 62-83% via RNAi, mutant cells exhibited photosynthetic oxygen evolution (POE) rates that were 68-100% higher than wild-type (WT) at pH 6...
November 7, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28169024/a-sustainable-use-of-ricotta-cheese-whey-for-microbial-biodiesel-production
#18
Eleonora Carota, Silvia Crognale, Alessandro D'Annibale, Anna Maria Gallo, Silvia Rita Stazi, Maurizio Petruccioli
The increasing demand of plant oils for biodiesel production has highlighted the need for alternative strategies based either on non-food crops or agro-industrial wastes that do not compete with food and feed production. In this context, the combined use of wastewater and oleaginous microorganisms could be a valuable production option. Ricotta cheese whey (RCW), one of the major byproducts of the dairy industry, is produced in very high and steadily increasing amounts and, due to its high organic load, its disposal is cost-prohibitive...
April 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28168314/cocoa-butter-like-lipid-production-ability-of-non-oleaginous-and-oleaginous-yeasts-under-nitrogen-limited-culture-conditions
#19
Yongjun Wei, Verena Siewers, Jens Nielsen
Cocoa butter (CB) extracted from cocoa beans is the main raw material for chocolate production. However, growing chocolate demands and limited CB production has resulted in a shortage of CB supply. CB is mainly composed of three different kinds of triacylglycerols (TAGs), POP (C16:0-C18:1-C16:0), POS (C16:0-C18:1-C18:0), and SOS (C18:0-C18:1-C18:0). The storage lipids of yeasts, mainly TAGs, also contain relative high-level of C16 and C18 fatty acids and might be used as CB-like lipids (CBL). In this study, we cultivated six different yeasts, including one non-oleaginous yeast strain, Saccharomyces cerevisiae CEN...
February 6, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28155157/nile-red-staining-of-neutral-lipids-in-yeast
#20
Kerry Ann Rostron, Clare Louise Lawrence
Determination of cellular neutral lipid levels in yeast is important for both the biotechnology industry and biomedical research. However, many of the currently available methods are labor intensive and time consuming. Here we describe a rapid and repeatable method for the detection of neutral lipids, which can be utilized in both oleaginous and non-oleaginous yeast species. The method utilizes the fluorescent dye, Nile red, which enables neutral lipid levels to either be visualized via microscopy or quantified using a 96-well plate assay...
2017: Methods in Molecular Biology
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