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https://www.readbyqxmd.com/read/28812146/engineering-rhodosporidium-toruloides-for-the-production-of-very-long-chain-monounsaturated-fatty-acid-rich-oils
#1
Sandy Fillet, Carmen Ronchel, Carla Callejo, María-José Fajardo, Helena Moralejo, José L Adrio
Erucic acid (cis-docosa-13-enoic acid, C22:1∆13) and nervonic acid (cis-tetracosa-15-enoic acid, C24:1 ∆15) are important renewable feedstocks in plastic, cosmetic, nylon, and lubricant industries. Furthermore, nervonic acid is also applied to the treatment of some neurological diseases. However, the production of these two very long-chain fatty acids (VLCFA) is very limited as both are not present in the main vegetable oils (e.g., soybean, rapeseed, sunflower, and palm). Ectopic integration and heterologous expression of fatty acid elongases (3-ketoacyl-CoA synthases, KCS) from different plants in Rhodosporidium toruloides resulted in the de novo synthesis of erucic acid and nervonic acid in this oleaginous yeast...
August 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28810867/new-inducible-promoter-for-gene-expression-and-synthetic-biology-in-yarrowia-lipolytica
#2
Marion Trassaert, Marie Vandermies, Fréderic Carly, Olivia Denies, Stéphane Thomas, Patrick Fickers, Jean-Marc Nicaud
BACKGROUND: The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed based either on the constitutive pTEF promoter or on oleic acid inducible promoters such as pPOX2 and pLIP2. Although these promoters are highly efficient, there is still a lack of versatile inducible promoters for gene expression in Y. lipolytica. RESULTS: We have isolated and characterized the promoter of the EYK1 gene coding for an erythrulose kinase...
August 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28810217/porous-cellulose-as-promoter-of-oil-production-by-the-oleaginous-yeast-lipomyces-starkeyi-using-mixed-agroindustrial-wastes
#3
Vassilios Ganatsios, Athanasios A Koutinas, Argyro Bekatorou, Vassilios Panagopoulos, Ibrahim M Banat, Antonia Terpou, Nikolaos Kopsahelis
Enhanced single cell oil (SCO) production by the oleaginous yeast Lipomyces starkeyi DSM 70296, immobilised on delignified porous cellulose, is reported. Pure glucose media were initially used. The effects of substrate pH and treatment temperature were evaluated, showing that 30°C and pH 5.0 were the optimum conditions for SCO production by the immobilised yeast. The immobilisation technique led to increased lipid accumulation and cell growth by 44% and 8%, respectively, in the glucose media, compared to free cells in suspension...
July 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28801795/storage-lipid-and-polysaccharide-metabolism-in-yarrowia-lipolytica-and-umbelopsis-isabellina
#4
Marianna Dourou, Panagiotis Mizerakis, Seraphim Papanikolaou, George Aggelis
Complex biochemical mechanisms are being involved in oleaginous microorganisms during storage lipid and polysaccharide metabolism. Detailed biochemical analyses and monitoring of key enzymes involved in carbon metabolism were performed in Yarrowia lipolytica and Umbelopsis isabellina, which are often used as model oleaginous microorganisms. It was found that during the early oleaginous phase, the carbon source (glucose) was channeled to lipid accumulation, but also to polysaccharide biosynthesis. However, during transition from the early to the late oleaginous phase, glucose was exclusively converted to lipids, while in U...
August 11, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28794923/a-sustainable-use-of-low-cost-raw-substrates-for-biodiesel-production-by-the-oleaginous-yeast-wickerhamomyces-anomalus
#5
Fatma Arous, Imen Ben Atitallah, Moncef Nasri, Tahar Mechichi
Over the past decade, the increasing demand of vegetable oils for biodiesel production has highlighted the need for alternative oil feedstocks that do not compete with food production. In this context, the combined use of agro-industrial wastes and oleaginous microorganisms could be a promising strategy for sustainable biodiesel production. The present investigation involves the performance of the oleaginous yeast Wickerhamomyces anomalus strain EC28 to produce lipids from different agro-industrial wastewaters (i...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28762006/oleaginous-microalgae-from-dairy-farm-wastewater-for-biodiesel-production-isolation-characterization-and-mass-cultivation
#6
Zheng Sun, Xiao-Peng Fang, Xiao-Yang Li, Zhi-Gang Zhou
Producing biodiesel from microalgae grown in wastewater is environment-friendly and cost-effective. The present study investigated the algae found in wastewater of a local dairy farm for their potential as biodiesel feedstocks. Thirteen native algal strains were isolated. On the basis of morphology and 16S/18S rRNA gene sequences, one strain was identified to be a member of cyanobacteria, while other 12 strains belong to green algae. After screening, two Scenedesmus strains out of the 13 microalgae isolates demonstrated superiority in growth rate, lipid productivity, and sedimentation properties, and therefore were selected for further scale-up outdoor cultivation...
July 31, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28760603/simultaneous-determination-of-intracellular-nucleotides-and-coenzymes-in-yarrowia-lipolytica-producing-lipid-and-lycopene-by-capillary-zone-electrophoresis
#7
Chen Zhao, Yan Yang, Linfang Wei, Qinghua Guo, Hao Fang, Shaolin Chen, Qiang Hua
Yarrowia lipolytica is an oleaginous yeast with promise in producing terpenoids such as lycopene. Though methods for analyzing primary metabolic intermediates have been established, further work is needed to better analyze nucleotides and coenzymes. Here, we presented an optimized method for the separation of nucleotides and coenzymes in Y. lipolytica using the capillary electrophoresis. The separation of twelve metabolites including four coenzymes, five nucleotides and three nucleosides was achieved within 32min using a voltage of 15kV and 70mM sodium carbonate/hydrogencarbonate buffer with 1...
July 24, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28752265/the-augmented-lipid-productivity-in-an-emerging-oleaginous-model-alga-coccomyxa-subellipsoidea-by-nitrogen-manipulation-strategy
#8
Chao Wang, Zhenyao Wang, Fei Luo, Yuqin Li
The lipid productivity controlled by both of biomass and lipid content was really crucial for economic-feasibility of microalgae-based biofuels production. This study attempted at augmenting lipid productivity in an emerging oleaginous model alga Coccomyxa subellipsoidea by different nitrogen manipulation including one-stage continuous N-sufficiency (OCNS), N-deprivation (OCND), N-limitation (OCNL), and also two-stage batch N-starvation (TBNS). Amongst four tested nitrogen manipulation strategies, OCNS performed remarkable promoting effect on cell metabolic growth and the maximum biomass was achieved by 7...
August 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28738851/expression-and-secretion-of-fungal-endoglucanase-ii-and-chimeric-cellobiohydrolase-i-in-the-oleaginous-yeast-lipomyces-starkeyi
#9
Qi Xu, Eric P Knoshaug, Wei Wang, Markus Alahuhta, John O Baker, Shihui Yang, Todd Vander Wall, Stephen R Decker, Michael E Himmel, Min Zhang, Hui Wei
BACKGROUND: Lipomyces starkeyi is one of the leading lipid-producing microorganisms reported to date; its genetic transformation was only recently reported. Our aim is to engineer L. starkeyi to serve in consolidated bioprocessing (CBP) to produce lipid or fatty acid-related biofuels directly from abundant and low-cost lignocellulosic substrates. RESULTS: To evaluate L. starkeyi in this role, we first conducted a genome analysis, which revealed the absence of key endo- and exocellulases in this yeast, prompting us to select and screen four signal peptides for their suitability for the overexpression and secretion of cellulase genes...
July 24, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28736146/lipid-accumulation-potential-of-oleaginous-yeasts-a-comparative-evaluation-using-food-waste-leachate-as-a-substrate
#10
Davidraj Johnravindar, Obulisamy Parthiba Karthikeyan, Ammaiyappan Selvam, Kumarasamy Murugesan, Jonathan W C Wong
In present study, the efficiency of three oleaginous yeasts i.e., Yarrowia lipolytica, Rhodotorula glutinis and Cryptococcus curvatus were compared for their lipid assimilation capacities using three different FW-leachates as a medium. The FW-leachates were collected from dry anaerobic digesters and diluted to achieve carbohydrate content of 25gL(-1) prior to yeast inoculations. Around 5% of yeast cultures were individually mixed in three different FW-leachate mediums and incubated under 30°C and 150rpm agitation for 6days...
June 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28731568/a-strong-hybrid-fatty-acid-inducible-transcriptional-sensor-built-from-yarrowia-lipolytica-upstream-activating-and-regulatory-sequences
#11
Murtaza Shabbir-Hussain, Ian Wheeldon, Mark A Blenner
The engineering of Yarrowia lipolytica to accumulate lipids with high titers and productivities has been enabled with a handful of constitutive promoters for pathway engineering. However, the development of promoters that are both strong and lipid responsive could greatly benefit the bioproduction efficiency of lipid-derived oleochemicals in oleaginous yeast. in this study, we sought to engineer a fatty acid regulated hybrid promoter for use in Y. lipolytica. We identified a 200 bp upstream regulatory sequence in the peroxisomal acyl CoA oxidase 2 (POX2) promoter...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28721723/enrichment-of-long-chain-polyunsaturated-fatty-acids-by-coordinated-expression-of-multiple-metabolic-nodes-in-the-oleaginous-microalga-phaeodactylum-tricornutum
#12
Xiang Wang, Yu-Hong Liu, Wei Wei, Xia Zhou, Wasiqi Yuan, Srinivasan Balamurugan, Ting-Bin Hao, Wei-Dong Yang, Jie-Sheng Liu, Hong-Ye Li
Microalgal long-chain polyunsaturated fatty acids (LC-PUFAs) have emerged as promising alternatives to depleting fish oils. However, the overproduction of LC-PUFAs in microalgae has remained challenging. Here, we report a sequential metabolic engineering strategy that systematically overcomes the metabolic bottlenecks and overproduces LC-PUFAs. Malonyl CoA-acyl carrier protein transacylase, catalyzing the first committed step in type II fatty acid synthesis, and desaturase 5b, involved in fatty acid desaturation, were coordinately expressed in Phaeodactylum tricornutum...
July 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28711832/biodegradation-of-phenol-via-meta-cleavage-pathway-triggers-de-novo-tag-biosynthesis-pathway-in-oleaginous-yeast
#13
Alok Patel, Km Sartaj, Neha Arora, Vikas Pruthi, Parul A Pruthi
Phenol is reported to be one of the most toxic environmental pollutants present in the discharge of various industrial effluents causing a serious threat to the existing biome. Biodegradation of phenol by oleaginous yeast Rhodosporidium kratochvilovae HIMPA1 was found to degrade 1000mg/l phenol. The pathways for phenol degradation by both ortho and meta-cleavage were proposed by the identification of metabolites and enzymatic assays of ring cleavage enzymes in the cell extracts. Results suggest that this oleaginous yeast degrade phenol via meta-cleavage pathway and accumulates a high quantity of lipid content (64...
July 6, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28706285/transcriptional-regulation-of-cellulose-biosynthesis-during-the-early-phase-of-nitrogen-deprivation-in-nannochloropsis-salina
#14
Seok Won Jeong, Seung Won Nam, Kwon HwangBo, Won Joong Jeong, Byeong-Ryool Jeong, Yong Keun Chang, Youn-Il Park
Microalgal photosynthesis provides energy and carbon-containing precursors for the biosynthesis of storage carbohydrates such as starch, chrysolaminarin, lipids, and cell wall components. Under mild nitrogen deficiency (N-), some Nannochloropsis species accumulate lipid by augmenting cytosolic fatty acid biosynthesis with a temporary increase in laminarin. Accordingly, biosynthesis of the cellulose-rich cell wall should change in response to N- stress because this biosynthetic pathway begins with utilisation of the hexose phosphate pool supplied from photosynthesis...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694845/a-type-i-diacylglycerol-acyltransferase-modulates-triacylglycerol-biosynthesis-and-fatty-acid-composition-in-the-oleaginous-microalga-nannochloropsis-oceanica
#15
Hehong Wei, Ying Shi, Xiaonian Ma, Yufang Pan, Hanhua Hu, Yantao Li, Ming Luo, Henri Gerken, Jin Liu
BACKGROUND: Photosynthetic oleaginous microalgae are considered promising feedstocks for biofuels. The marine microalga, Nannochloropsis oceanica, has been attracting ever-increasing interest because of its fast growth, high triacylglycerol (TAG) content, and available genome sequence and genetic tools. Diacylglycerol acyltransferase (DGAT) catalyzes the last and committed step of TAG biosynthesis in the acyl-CoA-dependent pathway. Previous studies have identified 13 putative DGAT-encoding genes in the genome of N...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28684180/enhancing-linalool-production-by-engineering-oleaginous-yeast-yarrowia-lipolytica
#16
Xuan Cao, Liu-Jing Wei, Jia-Yu Lin, Qiang Hua
In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20(F88W-N119W) gene resulted in further linalool production...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28681845/bacterial-lipid-droplets-bind-to-dna-via-an-intermediary-protein-that-enhances-survival-under-stress
#17
Congyan Zhang, Li Yang, Yunfeng Ding, Yang Wang, Lan Lan, Qin Ma, Xiang Chi, Peng Wei, Yongfang Zhao, Alexander Steinbüchel, Hong Zhang, Pingsheng Liu
Lipid droplets (LDs) are multi-functional organelles consisting of a neutral lipid core surrounded by a phospholipid monolayer, and exist in organisms ranging from bacteria to humans. Here we study the functions of LDs in the oleaginous bacterium Rhodococcus jostii. We show that these LDs bind to genomic DNA through the major LD protein, MLDS, which increases survival rate of the bacterial cells under nutritional and genotoxic stress. MLDS expression is regulated by a transcriptional regulator, MLDSR, that binds to the operator and promoter of the operon encoding both proteins...
July 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/28673516/metabolic-engineering-of-oleaginous-fungus-mortierella-alpina-for-high-production-of-oleic-and-linoleic-acids
#18
Takaiku Sakamoto, Eiji Sakuradani, Tomoyo Okuda, Hiroshi Kikukawa, Akinori Ando, Shigenobu Kishino, Yoshihiro Izumi, Takeshi Bamba, Jun Shima, Jun Ogawa
The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68...
June 19, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28666148/corn-bran-bioprocessing-development-of-an-integrated-process-for-microbial-lipids-production
#19
Jung-Eun Lee, Praveen V Vadlani, Jon Faubion
This study investigated the potential of corn bran as a feedstock for microbial lipid production using oleaginous yeast, Trichosporon oleaginosus ATCC20509. Different conditions (solid loading of biomass, acid loading, and pretreatment duration) were applied to optimize pretreatment processes using the Box-Behnken design. The highest sugar yield of 0.53g/g was obtained from corn bran hydrolysates at a pretreatment condition of 5% solid loading and 1% acid loading for 30min. Compared with synthetic media, up to 50% higher lipid accumulations in T...
June 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28665433/fast-microwave-assisted-acidolysis-a-new-biorefinery-approach-for-the-zero-waste-utilisation-of-lignocellulosic-biomass-to-produce-high-quality-lignin-and-fermentable-saccharides
#20
Long Zhou, Fabio Santomauro, Jiajun Fan, Duncan Macquarrie, James Clark, Christopher J Chuck, Vitaliy Budarin
Generally, biorefineries convert lignocellulosic biomass into a range of biofuels and further value added chemicals. However, conventional biorefinery processes focus mainly on the cellulose and hemicellulose fractions and therefore produce only low quality lignin, which is commonly burnt to provide process heat. To make full use of the biomass, more attention needs to be focused on novel separation techniques, where high quality lignin can be isolated that is suitable for further valorisation into aromatic chemicals and fuel components...
June 30, 2017: Faraday Discussions
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