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Jiaxin Chen, Xiaolei Zhang, Rajeshwar Dayal Tyagi, Patrick Drogui
In this work, methanol in crude glycerol solution was used to assist the lipid production with oleaginous yeast Trichosporon oleaginosus cultivated under non-sterilized conditions. The investigated methanol concentration was 0%, 1.4%, 2.2%, 3.3% and 4.4% (w/v). The results showed that methanol played a significant role in the non-sterilized fermentation for lipid production. The optimal methanol concentration was around 1.4% (w/v) in which the growth of T. oleaginosus was promoted and overcame that of the contaminants...
January 3, 2018: Bioresource Technology
Jiaxin Chen, Rajeshwar Dayal Tyagi, Ji Li, Xiaolei Zhang, Patrick Drogui, Feiyun Sun
Currently, there are mainly two pathways of the biodiesel production from wastewater sludge including 1) directly extracting the lipid in sludge and then converting the lipid to biodiesel through trans-esterification, and 2) employing sludge as medium to cultivate oleaginous microorganism to accumulate lipid and then transferring the lipid to biodiesel. So far, the study was still in research stage and its cost feasibility was not yet investigated. In this study, biodiesel production from wastewater sludge was designed and the cost was estimated with SuperPro Designer...
January 4, 2018: Bioresource Technology
Lauryn G Chan, Joshua L Cohen, Juliana Maria Leite Nobrega de Moura Bell
The design of new food products and increased agricultural activities have produced a diversity of waste streams or by-products that contain a high load of organic matter. The underutilization of these streams presents a serious threat to the environment and to the financial viability of the agricultural sector and the food industry. Oleaginous microorganisms, such as yeast and microalgae, have been used to convert the organic matter present in many agricultural waste streams into an oil-rich biomass. Filamentous fungi are promising oleaginous microorganisms because of their high lipid accumulation potential and simple biomass recovery, the latter being related to their pellet-like growth morphology in submerged cultivation...
January 12, 2018: Annual Review of Food Science and Technology
Hong-Yu Ren, Fanying Kong, Jun Ma, Lei Zhao, Guo-Jun Xie, Defeng Xing, Wan-Qian Guo, Bing-Feng Liu, Nan-Qi Ren
Synergistic system of dark fermentation and algal culture was initially operated at batch mode to investigate the energy production and nutrients removal from molasses wastewater in butyrate-type, ethanol-type and propionate-type fermentations. Butyrate-type fermentation was the most appropriate fermentation type for the synergistic system and exhibited the accumulative hydrogen volume of 658.3 mL L-1 and hydrogen yield of 131.7 mL g-1 COD. By-products from dark fermentation (mainly acetate and butyrate) were further used to cultivate oleaginous microalgae...
December 29, 2017: Bioresource Technology
Carlos Eduardo Robles-Rodríguez, Rafael Muñoz-Tamayo, Carine Bideaux, Nathalie Gorret, Stéphane E Guillouet, Carole Molina-Jouve, Gilles Roux, César Arturo Aceves-Lara
Oleaginous yeasts have been seen as a feasible alternative to produce the precursors of biodiesel due to their capacity to accumulate lipids as triacylglycerol having profiles with high content of unsaturated fatty acids. The yeast Yarrowia lipolytica is a promising microorganism that can produce lipids under nitrogen depletion conditions and excess of the carbon source. However, under these conditions, this yeast also produces citric acid (overflow metabolism) decreasing lipid productivity. This work presents two mathematical models for lipid production by Yarrowia lipolytica from glucose...
December 30, 2017: Biotechnology and Bioengineering
Cory Schwartz, Keith Frogue, Joshua Misa, Ian Wheeldon
Carotenoids are a class of molecules with commercial value as food and feed additives with nutraceutical properties. Shifting carotenoid synthesis from petrochemical-based precursors to bioproduction from sugars and other biorenewable carbon sources promises to improve process sustainability and economics. In this work, we engineered the oleaginous yeast Yarrowia lipolytica to produce the carotenoid lycopene. To enhance lycopene production, we tested a series of strategies to modify host cell physiology and metabolism, the most successful of which were mevalonate pathway overexpression and alleviating auxotrophies previously engineered into the PO1f strain of Y...
2017: Frontiers in Microbiology
Luning Zhang, Huaiyuan Zhang, Yuanda Song
Acyl-CoA:diacylglycerol acyltransferase (DGAT) is an pivotal regulator of triacylglycerol (TAG) synthesis. The oleaginous fungus Mucor circinelloides has four putative DGATs, McDGAT1A, McDGAT1B, McDGAT2A and McDGAT2B, classified into the DGAT1 and DGAT2 subfamilies, respectively. To identify and characterize DGATs in M. circinelloides, these four genes were expressed in Saccharomyces cerevisiae H1246 (TAG-deficient quadruple mutant), individually. TAG biosynthesis was restored only by the expression of McDGAT2B, and TAG content was significantly higher in the mutants with McDGAT2B expression than in a S...
December 20, 2017: Journal of Agricultural and Food Chemistry
Iuliia Ielchishcheva, Barbara Stachowiak, Artur Szwengiel, Anatoliy Bozhkov
The oleaginous yeast Rhodosporidium diobovatum is a poorly described producer of carotenoids and may be of interest in biotechnology. This study investigated the effects of culture medium and illumination on effective carotenoid production by R. diobovatum IMB Y-5023. Yeast was cultured on carrot, bran extract, and modified YM medium at illuminations in the 0-5,000 lx range. Biomass, total carotenoids, and their profile were assessed after cultivation. In order to samples comparison, cluster analysis (CA) and principle component analysis (PCA) were used to visualize the relationships between the variables and samples...
December 4, 2017: FEMS Microbiology Letters
Lynn Wong, Jake Engel, Erqing Jin, Benjamin Holdridge, Peng Xu
Effective metabolic engineering of microorganisms relies on balanced expression of both heterologous and endogenous genes to channel metabolic flux towards products of interest while achieving reasonable biomass buildup. To facilitate combinatorial pathway engineering and facile genetic operation, we engineered a set of modular cloning vectors compatible with BioBrick standards, called YaliBricks, to allow for rapid assembly of multigene pathways with customized genetic control elements (promoters, intronic sequences and terminators) in the oleaginous yeast Yarrowia lipolytica...
December 2017: Metabolic Engineering Communications
Pirapan Polburee, Takao Ohashi, Yung-Yu Tsai, Thitinun Sumyai, Noppon Lertwattanasakul, Savitree Limtong, Kazuhito Fujiyama
Triacylglycerol (TAG) is a major component of lipid storage in yeast. The acyl CoA: diacylgycerol acyltransferase (DGAT) that catalyzes the final and rate-limiting step in the production of TAG is rather interesting. Consequently, cloning and analysis of the gene-encoding TAG synthase, diacylglycerol acyltransferase gene (DGA1), of the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253 were undertaken. Analysis of the deduced amino acid sequence of DGA1 from R. fluvialis DMKU-RK253 (RfDGA1) showed similarity with the acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) from other organisms...
November 28, 2017: Microbiology
Miao Yang, Yan Fan, Pei-Chun Wu, Ya-Dong Chu, Pei-Li Shen, Song Xue, Zhan-You Chi
Microalgae represent a third generation biofuel feedstock due to their high triacylglycerol (TAG) content under adverse environmental conditions. Microalgal TAG resides in a single cell and serves as a lipid class mixed with complicated compositions. We previously showed that TAG possessed characteristic fatty acids (CFAs) for quantification and was linearly correlated with the relative abundance of CFA within certain limits in microalgae. Here, we defined the application range of the linear correlation between TAG and CFA in the oleaginous microalgae Chlamydomonas reinhardtii and Phaeodactylum tricornutum...
2017: Frontiers in Plant Science
Macrina Pérez-López, Rodolfo García-Contreras, Marcos Soto-Hernández, José Salud Rodríguez-Zavala, Mariano Martínez-Vázquez, Francisco Javier Prado-Galbarro, Israel Castillo-Juárez
Seed oils from oleaginous plants are rich in fatty acids (FAs) that play important roles in the health of the consumers. Recent studies indicate that FA also can play an important role in communication and regulation of virulence in bacteria. Nevertheless, evidence demonstrating protection against bacterial infections mediated by their quorum sensing inhibition (QSI) activity is scarce. In this study, sunflower, chia, and amaranth oils, were assayed for their QSI capacity by inhibiting violacein production and alkaline exoprotease activity of Chromobacterium violaceum...
November 27, 2017: Journal of Medicinal Food
Shuobo Shi, Huimin Zhao
Oleaginous yeasts have been increasingly explored for production of chemicals and fuels via metabolic engineering. Particularly, there is a growing interest in using oleaginous yeasts for the synthesis of lipid-related products due to their high lipogenesis capability, robustness, and ability to utilize a variety of substrates. Most of the metabolic engineering studies in oleaginous yeasts focused on Yarrowia that already has plenty of genetic engineering tools. However, recent advances in systems biology and synthetic biology have provided new strategies and tools to engineer those oleaginous yeasts that have naturally high lipid accumulation but lack genetic tools, such as Rhodosporidium, Trichosporon, and Lipomyces...
2017: Frontiers in Microbiology
Chao Ma, Yan-Bo Zhang, Shih-Hsin Ho, De-Feng Xing, Nan-Qi Ren, Bing-Feng Liu
Background: The light/dark cycle is one of the most important factors affecting the microalgal growth and lipid accumulation. Biomass concentration and lipid productivity could be enhanced by optimization of light/dark cycles, and this is considered an effective control strategy for microalgal cultivation. Currently, most research on effects of light/dark cycles on algae is carried out under autotrophic conditions and little information is about the effects under mixotrophic cultivation...
2017: Biotechnology for Biofuels
Nianci Shi, Weian Mao, Xiaoxia He, Zhe Chi, Zhenming Chi, Guanglei Liu
Yarrowia lipolytica is a promising platform for the single cell oil (SCO) production. In this study, a transformant X+N8 in which exo- and endo-inulinase genes were co-expressed could produce an inulinase activity of 124.33 U/mL within 72 h. However, the inulinase activity of a transformant X2 carrying a single exo-inulinase gene was only 47.33 U/mL within 72 h. Moreover, the transformant X+N8 could accumulate 48.13% (w/w) SCO from inulin and the cell dry weight reached 13.63 g/L within 78 h, which were significantly higher than those of the transformant X2 (41...
November 18, 2017: Applied Biochemistry and Biotechnology
Seunghyun Ryu, Cong T Trinh
Pentoses including xylose and arabinose are the second-most prevalent sugars of lignocellulosic biomass that can be harnessed for biological conversion. Although Yarrowia lipolytica has emerged as a promising industrial microorganism for production of high-value chemicals and biofuels, its native pentose metabolism is poorly understood. Our previous study demonstrated that Y. lipolytica (ATCC MYA-2613) has endogenous enzymes for D-xylose assimilation, but inefficient xylitol dehydrogenase causes Y. lipolytica to assimilate xylose poorly...
November 17, 2017: Applied and Environmental Microbiology
Allison Yaguchi, Alana Robinson, Erin Mihealsick, Mark Blenner
BACKGROUND: The oleaginous yeast, Trichosporon oleaginosus, has been extensively studied for its ability to metabolize non-conventional feedstocks. These include phenol-containing waste streams, such as distillery wastewater, or streams consisting of non-conventional sugars, such as hydrolyzed biomass and various bagasse. An initial BLAST search suggests this yeast has putative aromatic metabolizing genes. Given the desirability to valorize underutilized feedstocks such as lignin, we investigated the ability of T...
November 17, 2017: Microbial Cell Factories
Gergely Kosa, Volha Shapaval, Achim Kohler, Boris Zimmermann
BACKGROUND: Analyses of substrate and metabolites are often bottleneck activities in high-throughput screening of microbial bioprocesses. We have assessed Fourier transform infrared spectroscopy (FTIR), in combination with high throughput micro-bioreactors and multivariate statistical analyses, for analysis of metabolites in high-throughput screening of microbial bioprocesses. In our previous study, we have demonstrated that high-throughput (HTS) FTIR can be used for estimating content and composition of intracellular metabolites, namely triglyceride accumulation in oleaginous filamentous fungi...
November 13, 2017: Microbial Cell Factories
Yong-Su Jin, Jamie Hd Cate
Production of biofuels from lignocellulosic biomass remains an unsolved challenge in industrial biotechnology. Efforts to use yeast for conversion face the question of which host organism to use, counterbalancing the ease of genetic manipulation with the promise of robust industrial phenotypes. Saccharomyces cerevisiae remains the premier host for metabolic engineering of biofuel pathways, due to its many genetic, systems and synthetic biology tools. Numerous engineering strategies for expanding substrate ranges and diversifying products of S...
November 8, 2017: Current Opinion in Chemical Biology
Sujit Sadashiv Jagtap, Christopher V Rao
The sugar alcohol D-arabitol is one of the Department of Energy's top twelve bio-based building block chemicals. In this study, we found that the oleaginous yeast Rhodosporidium toruloides IFO0880 produces D-arabitol during growth on xylose in nitrogen-rich medium. Efficient xylose utilization was a prerequisite for extracellular D-arabitol production. During growth in complex media, R. toruloides produced 22 ± 2, 32 ± 2, and 49 ± 2 g/L D-arabitol from 70, 105, and 150 g/L xylose, respectively. In addition, we found that R...
November 11, 2017: Applied Microbiology and Biotechnology
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