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https://www.readbyqxmd.com/read/28429279/improvement-of-lipid-production-by-the-oleaginous-yeast-rhodosporidium-toruloides-through-uv-mutagenesis
#1
Ryosuke Yamada, Tomomi Kashihara, Hiroyasu Ogino
Oleaginous yeasts are considered a promising alternative lipid source for biodiesel fuel production. In this study, we attempted to improve the lipid productivity of the oleaginous yeast Rhodosporidium toruloides through UV irradiation mutagenesis and selection based on ethanol and H2O2 tolerance or cerulenin, a fatty acid synthetase inhibitor. Glucose consumption, cell growth, and lipid production of mutants were evaluated. The transcription level of genes involved in lipid production was also evaluated in mutants...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28427626/manothermosonication-as-a-useful-tool-for-lipid-extraction-from-oleaginous-microorganisms
#2
A Meullemiestre, C Breil, M Abert-Vian, F Chemat
Manothermonication is a recognized and efficient method used for sterilization in food preservation. The synergistic effect of sonication combined with pressure and temperature allows enhancing the cavitation activity. Never employed for extraction, this study is about the transposition of this process as a tool of extraction. In this study, Rhodosporidium toruloides yeast was submitted to extraction by four modes of sonication, with a temperature ranged from 20 to 55°C and a pressure between 1 to 2bars. The lipids extraction yields were compared to the conventional maceration...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28419226/sugar-versus-fat-elimination-of-glycogen-storage-improves-lipid-accumulation-in-yarrowia-lipolytica
#3
Govindprasad Bhutada, Martin Kavšcek, Rodrigo Ledesma-Amaro, Stéphane Thomas, Gerald N Rechberger, Jean-Marc Nicaud, Klaus Natter
Triacylglycerol (TAG) and glycogen are the two major metabolites for carbon storage in most eukaryotic organisms. We investigated the glycogen metabolism of the oleaginous Y. lipolytica and found that this yeast accumulates up to 16% glycogen in its biomass. Assuming that elimination of glycogen synthesis would result in an improvement of lipid accumulation, we characterized and deleted the single gene coding for glycogen synthase, YlGSY1. The mutant was grown under lipogenic conditions with glucose and glycerol as substrates and we obtained up to 60% improvement in TAG accumulation compared to the wild type strain...
April 17, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28411053/fostering-triacylglycerol-accumulation-in-novel-oleaginous-yeast-cryptococcus-psychrotolerans-iitrfd-utilizing-groundnut-shell-for-improved-biodiesel-production
#4
Farha Deeba, Vikas Pruthi, Yuvraj S Negi
The investigation was carried out to examine the potential of triacylglycerol (TAG) accumulation by novel oleaginous yeast isolate Cryptococcus psychrotolerans IITRFD on utilizing groundnut shell acid hydrolysate (GSH) as cost-effective medium. The maximum biomass productivity and lipid productivity of 0.095±0.008g/L/h and 0.044±0.005g/L/h, respectively with lipid content 46% was recorded on GSH. Fatty acid methyl ester (FAME) profile obtained by GC-MS analysis revealed oleic acid (37.8%), palmitic (29.4%) and linoleic (32...
April 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28403867/accelerated-triacylglycerol-production-and-altered-fatty-acid-composition-in-oleaginous-microalga-neochloris-oleoabundans-by-overexpression-of-diacylglycerol-acyltransferase-2
#5
Paeka Klaitong, Sirirat Fa-Aroonsawat, Wipa Chungjatupornchai
BACKGROUND: Microalgae are promising sources of lipid triacylglycerol (TAG) for biodiesel production. However, to date, microalgal biodiesel is technically feasible, but not yet economically viable. Increasing TAG content and productivity are important to achieve economic viability of microalgal biodiesel. To increase TAG content, oleaginous microalga Neochloris oleoabundans was genetically engineered with an endogenous key enzyme diacylglycerol acyltransferase 2 (NeoDGAT2) responsible for TAG biosynthesis...
April 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28396881/allelopathic-activity-of-extracts-from-different-brazilian-peanut-arachis-hypogaea-l-cultivars-on-lettuce-lactuca-sativa-and-weed-plants
#6
G S Casimiro, E Mansur, G Pacheco, R Garcia, I C R Leal, N K Simas
Peanut (Arachis hypogaea L.) is the fourth most consumed oleaginous plant in the world, producing seeds with high contents of lipids, proteins, vitamins, and carbohydrates. Biological activities of different extracts of this species have already been evaluated by many researchers, including antioxidant, antitumoral, and antibacterial. In this work, the allelopathic activity of extracts from different Brazilian peanut cultivars against lettuce (Lactuca sativa) and two weed plants (Commelina benghalensis and Ipomoea nil) was studied...
2017: TheScientificWorldJournal
https://www.readbyqxmd.com/read/28386673/development-of-an-agrobacterium-mediated-transformation-method-and-evaluation-of-two-exogenous-constitutive-promoters-in-oleaginous-yeast-lipomyces-starkeyi
#7
Xinping Lin, Sasa Liu, Ruiqi Bao, Ning Gao, Sufang Zhang, Rongqian Zhu, Zongbao Kent Zhao
Oleaginous yeast Lipomyces starkeyi, a promising strain of great biotechnical importance, is able to accumulate over 60% of its cell biomass as triacylglycerols (TAGs). It is promising to directly produce the derivatives of TAGs, such as long-chain fatty acid methyl esters and alkanes, in L. starkeyi. However, techniques for genetic modification of this oleaginous yeast are lacking, thus, further research is needed to develop genetic tools and functional elements. Here, we used two exogenous promoters (pGPD and pPGK) from oleaginous yeast Rhodosporidium toruloides to establish a simpler Agrobacterium-mediated transformation (AMT) method for L...
April 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28385836/draft-genome-sequence-of-subantarctic-rhodococcus-sp-strain-1139
#8
Akhikun Nahar, Anthony L Baker, Michael A Charleston, Margaret L Britz
The draft genome sequence of subantarctic Rhodococcus sp. strain 1139 is reported here. The genome size is 7.04 Mb with high G+C content (62.3%) and it contains a large number of genes involved in lipid synthesis. This lipid synthesis system is characteristic of oleaginous Actinobacteria, which are of interest for biofuel production.
April 6, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28378827/real-time-itraq-based-proteome-profiling-revealed-the-central-metabolism-involved-in-nitrogen-starvation-induced-lipid-accumulation-in-microalgae
#9
Vineeta Rai, Muthusivaramapandian Muthuraj, Mayuri N Gandhi, Debasish Das, Sanjeeva Srivastava
To understand the post-transcriptional molecular mechanisms attributing to oleaginousness in microalgae challenged with nitrogen starvation (N-starvation), the longitudinal proteome dynamics of Chlorella sp. FC2 IITG was investigated using multipronged quantitative proteomics and multiple reaction monitoring assays. Physiological data suggested a remarkably enhanced lipid accumulation with concomitant reduction in carbon flux towards carbohydrate, protein and chlorophyll biosynthesis. The proteomics-based investigations identified the down-regulation of enzymes involved in chlorophyll biosynthesis (porphobilinogen deaminase) and photosynthetic carbon fixation (sedoheptulose-1,7 bisphosphate and phosphoribulokinase)...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28374798/dynamic-metabolic-profiling-together-with-transcription-analysis-reveals-salinity-induced-starch-to-lipid-biosynthesis-in-alga-chlamydomonas-sp-jsc4
#10
Shih-Hsin Ho, Akihito Nakanishi, Yuichi Kato, Hiroaki Yamasaki, Jo-Shu Chang, Naomi Misawa, Yuu Hirose, Jun Minagawa, Tomohisa Hasunuma, Akihiko Kondo
Biodiesel production using microalgae would play a pivotal role in satisfying future global energy demands. Understanding of lipid metabolism in microalgae is important to isolate oleaginous strain capable of overproducing lipids. It has been reported that reducing starch biosynthesis can enhance lipid accumulation. However, the metabolic mechanism controlling carbon partitioning from starch to lipids in microalgae remains unclear, thus complicating the genetic engineering of algal strains. We here used "dynamic" metabolic profiling and essential transcription analysis of the oleaginous green alga Chlamydomonas sp...
April 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28371542/evaluation-of-green-solvents-oil-extraction-from-oleaginous-yeast-lipomyces-starkeyi-using-cyclopentyl-methyl-ether-cpme
#11
Kyle V Probst, Michael D Wales, Mary E Rezac, Praveen V Vadlani
Cyclopentyl methyl ether (CPME) was evaluated for extracting oil or triacylglycerol (TAG) from wet cells of the oleaginous yeast Lipomyces starkeyi. CPME is a greener alternative to chloroform as a potential solvent for oil recovery. A monophasic system of CPME and biphasic system of CPME:water (1:0.7) performed poorly having the lowest TAG extraction efficiency and TAG selectivity compared to other monophasic systems of hexane and chloroform and the biphasic Bligh and Dyer method (chloroform:methanol:water)...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28369336/fast-and-efficient-genetic-transformation-of-oleaginous-yeast-rhodosporidium-toruloides-by-using-electroporation
#12
Hongdi Liu, Xiang Jiao, Yanan Wang, Xiaobing Yang, Wenyi Sun, Jihui Wang, Sufang Zhang, Zongbao Kent Zhao
Metabolic engineering of Rhodosporidium toruloides, a robust lipid and caroteinoid producer, is of great importance for oleochemicals and carotenoids production. However, the Agrobacterium-mediated gene transformation is tedious and time consuming. Here, we described a fast and efficient genetic transformation of R. toruloides using electroporation with linear DNA fragments, and the process was optimized. The results showed that 2 × 103 transformants can be obtained at 0.7 kV/μg linear DNA by using hygromycin and bleomycin as selection markers after the competent cells pretreated with 25 mM DTT and 100 mM LiAc...
March 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28366694/augmented-lipid-accumulation-in-ethyl-methyl-sulphonate-mutants-of-oleaginous-microalga-for-biodiesel-production
#13
Juhi Mehtani, Neha Arora, Alok Patel, Priyanka Jain, Parul A Pruthi, Kirshna Mohan Poluri, Vikas Pruthi
The aim of this work was to generate high lipid accumulating mutants of Chlorella minutissima (CM) using ethyl methyl sulphonate (EMS) as a random chemical mutagen. Amid the 5% surviving cells after exposure to EMS (2M), three fast growing mutants (CM2, CM5, CM7) were selected and compared with wild type for lipid productivity and biochemical composition. Among these mutants, CM7 showed the maximum biomass (2.4g/L) and lipid content (42%) as compared to wild type (1.5g/L; 27%). Further, the mutant showed high photosynthetic pigments with low starch content signifying the re-allocation of carbon flux to lipid...
March 21, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28357546/a-metabolic-engineering-strategy-for-producing-conjugated-linoleic-acids-using-the-oleaginous-yeast-yarrowia-lipolytica
#14
Nabila Imatoukene, Jonathan Verbeke, Athanasios Beopoulos, Abdelghani Idrissi Taghki, Brigitte Thomasset, Claude-Olivier Sarde, Maurice Nonus, Jean-Marc Nicaud
Conjugated linoleic acids (CLAs) have been found to have beneficial effects on human health when used as dietary supplements. However, their availability is limited because pure, chemistry-based production is expensive, and biology-based fermentation methods can only create small quantities. In an effort to enhance microbial production of CLAs, four genetically modified strains of the oleaginous yeast Yarrowia lipolytica were generated. These mutants presented various genetic modifications, including the elimination of β-oxidation (pox1-6∆), the inability to store lipids as triglycerides (dga1∆ dga2∆ are1∆ lro1∆), and the overexpression of the Y...
March 29, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28352301/functional-overexpression-of-genes-involved-in-erythritol-synthesis-in-the-yeast-yarrowia-lipolytica
#15
Aleksandra M Mirończuk, Anna Biegalska, Adam Dobrowolski
BACKGROUND: Erythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is a natural sweetener produced on an industrial scale for decades. Despite the fact that the yeast Yarrowia lipolytica has been reported since the 1970s as an erythritol producer, the metabolic pathway of this polyol has never been characterized. It was shown that erythritol synthesis in yeast occurs via the pentose phosphate pathway (PPP). The oleaginous yeast Y. lipolytica is a good host for converting inexpensive glycerol into a value-added product such as erythritol...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28346334/outdoor-cultivation-of-marine-diatoms-for-year-round-production-of-biofuels
#16
Mitsufumi Matsumoto, Daisuke Nojima, Tomomi Nonoyama, Kiichi Ikeda, Yoshiaki Maeda, Tomoko Yoshino, Tsuyoshi Tanaka
Biofuel production using microalgae is believed to have the advantage of continuous year-round production over crop plants, which have strong seasonality. However, actual year-round production of microalgal lipids using outdoor mass cultivation has rarely been demonstrated. In our previous study, it was demonstrated that the oleaginous diatom, Fistulifera solaris, was culturable in outdoor bioreactors from spring to autumn, whereas biomass and lipid production in winter failed because F. solaris did not grow below 15 °C...
March 25, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28345304/chemical-evidence-for-the-effect-of-urochloa-ruziziensis-on-glyphosate-resistant-soybeans
#17
Mariluce Nepomuceno, Nuria Chinchilla, Rosa M Varela, José M G Molinillo, Rodney Lacret, Pedro L C A Alves, Francisco A Macias
BACKGROUND: Soybean (Glycine max) is an important oleaginous legume that has been cultivated in new areas in Brazil, including pastures. Problems of reduced production yields have been reported by soybean growers when the crop is sown immediately after desiccation of pastures of Urochloa spp. using glyphosate. The objective of this work was to extract, isolate, and identify the major chemicals from U. ruziziensis that have phytotoxic activity and to evaluate the possible relation between this effect and the reduced soybean yield...
March 26, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28337556/homologous-gene-targeting-of-a-carotenoids-biosynthetic-gene-in-rhodosporidium-toruloides-by-agrobacterium-mediated-transformation
#18
Wenyi Sun, Xiaobing Yang, Xueying Wang, Xinping Lin, Yanan Wang, Sufang Zhang, Yushi Luan, Zongbao K Zhao
OBJECTIVES: To target a carotenoid biosynthetic gene in the oleaginous yeast Rhodosporidium toruloides by using the Agrobacterium-mediated transformation (AMT) method. RESULTS: The RHTO_04602 locus of R. toruloides NP11, previously assigned to code the carotenoid biosynthetic gene CRTI, was amplified from genomic DNA and cloned into the binary plasmid pZPK-mcs, resulting in pZPK-CRT. A HYG-expression cassette was inserted into the CRTI sequence of pZPK-CRT by utilizing the restriction-free clone strategy...
March 23, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28303649/transforming-sugars-into-fat-lipid-biosynthesis-using-different-sugars-in-yarrowia-lipolytica
#19
Piotr Hapeta, Magdalena Rakicka, Remi Dulermo, Heber Gamboa-Meléndez, Anne-Marie Cruz-Le Coq, Jean-Marc Nicaud, Zbigniew Lazar
In an era of ever-increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as Yarrowia lipolytica to convert waste materials into biofuels. Here, we constructed two Y. lipolytica strains that displayed both increased lipid accumulation and more efficient use of biomass-derived sugars, including glucose, fructose, galactose and inulin. The first strain, Y. lipolytica YLZ150, was derived from the French wild-type strain W29. It had inhibited triacylglycerol mobilization (∆tgl4) and β-oxidation (∆pox1-6), and it overexpressed GPD1, DGA2, HXK1, the native Leloir pathway, SUC2 from Saccharomyces cerevisiae and INU1 from Kluyveromyces marxianus...
March 17, 2017: Yeast
https://www.readbyqxmd.com/read/28302588/nuclear-transformation-and-functional-gene-expression-in-the-oleaginous-microalga-monoraphidium-neglectum
#20
Daniel Jaeger, Wolfgang Hübner, Thomas Huser, Jan H Mussgnug, Olaf Kruse
Photosynthetic microalgae hold great promise as non-food feedstocks for the sustainable production of a range of bio-products and genetic engineering is an increasingly important strategy to improve the natural cellular features. For the vast majority of microalgal strains, however, genetic engineering is not yet possible and the establishment of efficient genetic tools for a broad range of microalgal species is an important task to reach biotechnological success. Stable DNA transformation is one of the crucial steps for most genetic engineering approaches...
March 14, 2017: Journal of Biotechnology
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