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https://www.readbyqxmd.com/read/28924452/greenhouse-gas-performance-of-biochemical-biodiesel-production-from-straw-soil-organic-carbon-changes-and-time-dependent-climate-impact
#1
Hanna Karlsson, Serina Ahlgren, Mats Sandgren, Volkmar Passoth, Ola Wallberg, Per-Anders Hansson
BACKGROUND: Use of bio-based diesel is increasing in Europe. It is currently produced from oilseed crops, but can also be generated from lignocellulosic biomass such as straw. However, removing straw affects soil organic carbon (SOC), with potential consequences for the climate impact of the biofuel. This study assessed the climate impacts and energy balance of biodiesel production from straw using oleaginous yeast, with subsequent biogas production from the residues, with particular emphasis on SOC changes over time...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28918933/structural-proteomics-topology-and-relative-accessibility-of-plant-lipid-droplet-associated-proteins
#2
Pascale Jolivet, Laure Aymé, Alexandre Giuliani, Frank Wien, Thierry Chardot, Yann Gohon
Lipid droplets are the major stock of lipids in oleaginous plant seeds. Despite their economic importance for oil production and biotechnological issues (biofuels, lubricants and plasticizers), numerous questions about their formation, structure and regulation are still unresolved. To determine water accessible domains of protein coating at lipid droplets surface, a structural proteomic approach has been performed. This technique relies on the millisecond timescale production of hydroxyl radicals by the radiolysis of water using Synchrotron X-ray white beam...
September 14, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28918245/biodiesel-production-from-lipid-of-carbon-dioxide-sequestrating-bacterium-and-lipase-of-psychrotolerant-pseudomonas-sp-istpl3-immobilized-on-biochar
#3
Khushboo Khosla, Rashmi Rathour, Raj Maurya, Neha Maheshwari, Edgard Gnansounou, Christian Larroche, Indu Shekhar Thakur
An extracellular lipase was purified and characterized from psychrotolerant bacterium Pseudomonas sp. ISTPL3 isolated from Pangong lake. Lipase was purified by sequential methods of ammonium sulphate precipitation, dialysis, DEAE-cellulose ion exchange chromatography and Sephadex G-100 gel filtration chromatography, resulting in a purification fold of 6.53 and yield of 5.45%. The molecular weight was approximately 31kDa. The purified lipase was used for transesterification of lipids produced by oleaginous chemolithotrophic bacterium Serratia sp...
September 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28904365/rnai-mediated-silencing-of-a-pyruvate-dehydrogenase-kinase-enhances-triacylglycerol-biosynthesis-in-the-oleaginous-marine-alga-nannochloropsis-salina
#4
Xiaonian Ma, Lina Yao, Bo Yang, Yuan Kun Lee, Feng Chen, Jin Liu
Oleaginous microalgae have been emerging as the third-generation feedstocks for biofuel production. Genetic manipulation for improving triacylglycerol (TAG) accumulation represents a promising approach towards the economics of microalgal biofuels. Acetyl-CoA, the essential carbon precursor for de novo fatty acid biosynthesis, can be derived from pyruvate catalyzed by pyruvate dehydrogenase, which is negatively regulated by pyruvate dehydrogenase kinase (PDK). In the present study, we characterized a PDK gene (NsPDK) from Nannochloropsis salina...
September 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28900030/multiple-mechanisms-contribute-to-increased-neutral-lipid-accumulation-in-yeast-producing-recombinant-variants-of-plant-diacylglycerol-acyltransferase-1
#5
Yang Xu, Guanqun Chen, Michael S Greer, Kristian Mark P Caldo, Geetha Ramakrishnan, Saleh Shah, Limin Wu, M Joanne Lemieux, Jocelyn Ozga, Randall J Weselake
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT activity using protein engineering could lead to improvements in seed oil yield, for example in canola-type Brassica napus. Directed evolution of B. napus DGAT1 (BnaDGAT1) previously revealed that one of the regions where amino acid residue substitutions leading to higher performance in BnaDGAT1 was in the ninth predicted transmembrane domain (PTMD9)...
September 12, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28898857/production-of-eicosapentaenoic-acid-by-high-cell-density-cultivation-of-the-marine-oleaginous-diatom-fistulifera-solaris
#6
Tsuyoshi Tanaka, Takashi Yabuuchi, Yoshiaki Maeda, Daisuke Nojima, Mitsufumi Matsumoto, Tomoko Yoshino
Polyunsaturated fatty acids (PUFAs), including eicosapentaenoic acid (EPA), have attracted attention owing to their health benefits for humans, as well as their importance in aquaculture and animal husbandry. Establishing a sustainable PUFA supply based on fish oils has been difficult due to their increasing demand. Therefore, alternative sources of PUFAs are required. In this research, we examined the potential of the marine oleaginous diatom Fistulifera solaris as an alternative producer of PUFAs. Optimization of culture conditions was carried out for high cell density cultivation, and a maximal biomass productivity of 1...
September 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28892702/production-of-wax-esters-via-microbial-oil-synthesis-from-food-industry-waste-and-by-product-streams
#7
Aikaterini Papadaki, Athanasios Mallouchos, Maria-Nefeli Efthymiou, Chryssavgi Gardeli, Nikolaos Kopsahelis, Erika C G Aguieiras, Denise M G Freire, Seraphim Papanikolaou, Apostolis A Koutinas
The production of wax esters using microbial oils was demonstrated in this study. Microbial oils produced from food waste and by-product streams by three oleaginous yeasts were converted into wax esters via enzymatic catalysis. Palm oil was initially used to evaluate the influence of temperature and enzyme activity on wax ester synthesis catalysed by Novozyme 435 and Lipozyme lipases using cetyl, oleyl and behenyl alcohols. The highest conversion yields (up to 79.6%) were achieved using 4U/g of Novozyme 435 at 70°C...
August 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28870008/oleaginous-yeast-rhodosporidium-toruloides-as-a-tool-for-rapid-evaluation-of-anti-obesity-candidates-inhibitory-effect-of-persimmon-leaf-fermentate-on-lipid-accumulation
#8
Nam Keun Lee
The aim of this study was to examine the efficiency of Rhodosporidium toruloides as a new tool to evaluate the triglyceride (TG) reduction effects of anti-obesity candidate materials. Unfermented and fermented persimmon leaf hot water extracts (UFPLE and FPLE) were used as anti-obesity agents. The content of TG in R. toruloides treated with FPLE was less than those with UFPLE by about 11% (p < 0.05) compared to the control (R. toruloides incubated in YPD medium without the agents). Fat reduction in 3T3-L1 cells achieved FPLE was about 13% higher than that achieved by UFPLE...
September 5, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28865010/genome-characterization-of-oleaginous-aspergillus-oryzae-bcc7051-a-potential-fungal-based-platform-for-lipid-production
#9
Chinae Thammarongtham, Intawat Nookaew, Tayvich Vorapreeda, Tanawut Srisuk, Miriam L Land, Sukanya Jeennor, Kobkul Laoteng
The selected robust fungus, Aspergillus oryzae strain BCC7051 is of interest for biotechnological production of lipid-derived products due to its capability to accumulate high amount of intracellular lipids using various sugars and agro-industrial substrates. Here, we report the genome sequence of the oleaginous A. oryzae BCC7051. The obtained reads were de novo assembled into 25 scaffolds spanning of 38,550,958 bps with predicted 11,456 protein-coding genes. By synteny mapping, a large rearrangement was found in two scaffolds of A...
September 1, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28857665/efficient-bioconversion-from-acid-hydrolysate-of-waste-oleaginous-yeast-biomass-after-microbial-oil-extraction-to-bacterial-cellulose-by-komagataeibacter-xylinus
#10
Mu-Tan Luo, Chao Huang, Xue-Fang Chen, Qian-Lin Huang, Gao-Xiang Qi, Lan-Lan Tian, Lian Xiong, Hai-Long Li, Xin-De Chen
Biomass acid hydrolysate of oleaginous yeast Trichosporon cutaneum after microbial oil extraction was applied as substrate for bacterial cellulose (BC) production by Komagataeibacter xylinus (also named as Gluconacetobacter xylinus previously) for the first time. BC was synthesized in static culture for ten days and the maximum BC yield (2.9 g/ L) was got at the 4th day of fermentation. Most carbon sources in the substrate (glucose, mannose, formic acid, acetic acid, etc.) can be utilized by K. xylinus...
August 31, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28852423/single-cell-oil-production-by-trichosporon-cutaneum-from-steam-exploded-corn-stover-and-its-upgradation-for-production-of-long-chain-%C3%AE-%C3%AF-dicarboxylic-acids
#11
Chen Zhao, Hao Fang, Shaolin Chen
BACKGROUND: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based bioprocess to more valuable ones is becoming a more and more important issue. RESULTS: Differently sourced cellulases were compared in the enzymatic hydrolysis of the steam-exploded corn stover (SECS) and the cellulase from the mixed culture of Trichoderma reesei and Aspergillus niger was found to have the highest enzymatic hydrolysis yield 86...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28832949/a-metabolic-engineering-strategy-for-producing-free-fatty-acids-by-the-yarrowia-lipolytica-yeast-based-on-impairment-of-glycerol-metabolism
#12
Evgeniya Y Yuzbasheva, Elizaveta B Mostova, Natalia I Andreeva, Tigran V Yuzbashev, Alexander S Fedorov, Irina A Konova, Sergey P Sineoky
In recent years, bio-based production of free fatty acids from renewable resources has attracted attention for their potential as precursors for the production of biofuels and biochemicals. In this study, the oleaginous yeast Yarrowia lipolytica was engineered to produce free fatty acids by eliminating glycerol metabolism. Free fatty acid production was monitored under lipogenic conditions with glycerol as a limiting factor. Firstly, the strain W29 (Δgpd1), which is deficient in glycerol synthesis, was obtained...
August 19, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28827048/bioconversion-of-biodiesel-derived-crude-glycerol-into-lipids-and-carotenoids-by-an-oleaginous-red-yeast-sporidiobolus-pararoseus-km281507-in-an-airlift-bioreactor
#13
Atchara Manowattana, Charin Techapun, Masanori Watanabe, Thanongsak Chaiyaso
Here we tested the bioconversion of biodiesel-derived crude glycerol by the oleaginous red yeast Sporidiobolus pararoseus KM281507 in two bioreactors types (stirred-tank and airlift). High production yields (biomass, 10.62 ± 0.21 g/L; lipids, 3.26 ± 0.13 g/L; β-carotene, 30.64 ± 0.05 mg/L; total carotenoids, 46.59 ± 0.07 mg/L) were achieved in a 3.0 L airlift bioreactor under uncontrolled pH regimes (initial pH 5.63). Under optimized conditions (6.0 vvm aeration rate; 60 ± 5% constant dissolved oxygen [DO] maintained by flushing pure oxygen [O2] into the vessel; 10,000 Lux light irradiation) volumetric production in the airlift bioreactor was further increased (biomass, 19...
August 18, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28819197/mortierella-alpina-cs10e4-an-oleaginous-fungal-endophyte-of-crocus-sativus-l-enhances-apocarotenoid-biosynthesis-and-stress-tolerance-in-the-host-plant
#14
Zahoor Ahmed Wani, Amit Kumar, Phalisteen Sultan, Kushal Bindu, Syed Riyaz-Ul-Hassan, Nasheeman Ashraf
Crocus sativus is the only plant species which produces apocarotenoids like crocin, picrocrocin and safranal in significant amounts. These compounds impart organoleptic properties to saffron (dried stigmas of Crocus flower) making it world's costliest spice. Crocus apocarotenoids have tremendous medicinal properties as well. Effect of endophytes on Crocus apocarotenoid production and the molecular mechanism involved has not been reported so far. Here we studied the effect of an oleaginous fungal endophyte, Mortierella alpina CS10E4 on Crocus growth, apocarotenoid metabolism and tolerance to corm rot disease...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28814974/time-resolved-transcriptome-analysis-and-lipid-pathway-reconstruction-of-the-oleaginous-green-microalga-monoraphidium-neglectum-reveal-a-model-for-triacylglycerol-and-lipid-hyperaccumulation
#15
Daniel Jaeger, Anika Winkler, Jan H Mussgnug, Jörn Kalinowski, Alexander Goesmann, Olaf Kruse
BACKGROUND: Oleaginous microalgae are promising production hosts for the sustainable generation of lipid-based bioproducts and as bioenergy carriers such as biodiesel. Transcriptomics of the lipid accumulation phase, triggered efficiently by nitrogen starvation, is a valuable approach for the identification of gene targets for metabolic engineering. RESULTS: An explorative analysis of the detailed transcriptional response to different stages of nitrogen availability was performed in the oleaginous green alga Monoraphidium neglectum...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28812146/engineering-rhodosporidium-toruloides-for-the-production-of-very-long-chain-monounsaturated-fatty-acid-rich-oils
#16
Sandy Fillet, Carmen Ronchel, Carla Callejo, María-José Fajardo, Helena Moralejo, José L Adrio
Erucic acid (cis-docosa-13-enoic acid, C22:1∆13) and nervonic acid (cis-tetracosa-15-enoic acid, C24:1 ∆15) are important renewable feedstocks in plastic, cosmetic, nylon, and lubricant industries. Furthermore, nervonic acid is also applied to the treatment of some neurological diseases. However, the production of these two very long-chain fatty acids (VLCFA) is very limited as both are not present in the main vegetable oils (e.g., soybean, rapeseed, sunflower, and palm). Ectopic integration and heterologous expression of fatty acid elongases (3-ketoacyl-CoA synthases, KCS) from different plants in Rhodosporidium toruloides resulted in the de novo synthesis of erucic acid and nervonic acid in this oleaginous yeast...
August 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28810867/new-inducible-promoter-for-gene-expression-and-synthetic-biology-in-yarrowia-lipolytica
#17
Marion Trassaert, Marie Vandermies, Fréderic Carly, Olivia Denies, Stéphane Thomas, Patrick Fickers, Jean-Marc Nicaud
BACKGROUND: The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed based either on the constitutive pTEF promoter or on oleic acid inducible promoters such as pPOX2 and pLIP2. Although these promoters are highly efficient, there is still a lack of versatile inducible promoters for gene expression in Y. lipolytica. RESULTS: We have isolated and characterized the promoter of the EYK1 gene coding for an erythrulose kinase...
August 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28810217/porous-cellulose-as-promoter-of-oil-production-by-the-oleaginous-yeast-lipomyces-starkeyi-using-mixed-agroindustrial-wastes
#18
Vassilios Ganatsios, Athanasios A Koutinas, Argyro Bekatorou, Vassilios Panagopoulos, Ibrahim M Banat, Antonia Terpou, Nikolaos Kopsahelis
Enhanced single cell oil (SCO) production by the oleaginous yeast Lipomyces starkeyi DSM 70296, immobilised on delignified porous cellulose, is reported. Pure glucose media were initially used. The effects of substrate pH and treatment temperature were evaluated, showing that 30°C and pH 5.0 were the optimum conditions for SCO production by the immobilised yeast. The immobilisation technique led to increased lipid accumulation and cell growth by 44% and 8%, respectively, in the glucose media, compared to free cells in suspension...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28801795/storage-lipid-and-polysaccharide-metabolism-in-yarrowia-lipolytica-and-umbelopsis-isabellina
#19
Marianna Dourou, Panagiotis Mizerakis, Seraphim Papanikolaou, George Aggelis
Complex biochemical mechanisms are being involved in oleaginous microorganisms during storage lipid and polysaccharide metabolism. Detailed biochemical analyses and monitoring of key enzymes involved in carbon metabolism were performed in Yarrowia lipolytica and Umbelopsis isabellina, which are often used as model oleaginous microorganisms. It was found that during the early oleaginous phase, the carbon source (glucose) was channeled to lipid accumulation, but also to polysaccharide biosynthesis. However, during transition from the early to the late oleaginous phase, glucose was exclusively converted to lipids, while in U...
August 11, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28794923/a-sustainable-use-of-low-cost-raw-substrates-for-biodiesel-production-by-the-oleaginous-yeast-wickerhamomyces-anomalus
#20
Fatma Arous, Imen Ben Atitallah, Moncef Nasri, Tahar Mechichi
Over the past decade, the increasing demand of vegetable oils for biodiesel production has highlighted the need for alternative oil feedstocks that do not compete with food production. In this context, the combined use of agro-industrial wastes and oleaginous microorganisms could be a promising strategy for sustainable biodiesel production. The present investigation involves the performance of the oleaginous yeast Wickerhamomyces anomalus strain EC28 to produce lipids from different agro-industrial wastewaters (i...
August 2017: 3 Biotech
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