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https://www.readbyqxmd.com/read/28731568/a-strong-hybrid-fatty-acid-inducible-transcriptional-sensor-built-from-yarrowia-lipolytica-upstream-activating-and-regulatory-sequences
#1
Murtaza Shabbir-Hussain, Ian Wheeldon, Mark A Blenner
The engineering of Yarrowia lipolytica to accumulate lipids with high titers and productivities has been enabled with a handful of constitutive promoters for pathway engineering. However, the development of promoters that are both strong and lipid responsive could greatly benefit the bioproduction efficiency of lipid-derived oleochemicals in oleaginous yeast. in this study, we sought to engineer a fatty acid regulated hybrid promoter for use in Y. lipolytica. We identified a 200 bp upstream regulatory sequence in the peroxisomal acyl CoA oxidase 2 (POX2) promoter...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28721723/enrichment-of-long-chain-polyunsaturated-fatty-acids-by-coordinated-expression-of-multiple-metabolic-nodes-in-the-oleaginous-microalga-phaeodactylum-tricornutum
#2
Xiang Wang, Yu-Hong Liu, Wei Wei, Xia Zhou, Wasiqi Yuan, Srinivasan Balamurugan, Ting-Bin Hao, Wei-Dong Yang, Jie-Sheng Liu, Hong-Ye Li
Microalgal long-chain polyunsaturated fatty acids (LC-PUFAs) have emerged as promising alternatives to depleting fish oils. However, the overproduction of LC-PUFAs in microalgae has remained challenging. Here, we report a sequential metabolic engineering strategy that systematically overcomes the metabolic bottlenecks and overproduces LC-PUFAs. Malonyl CoA-acyl carrier protein transacylase, catalyzing the first committed step in type II fatty acid synthesis, and desaturase 5b, involved in fatty acid desaturation, were coordinately expressed in Phaeodactylum tricornutum...
July 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28711832/biodegradation-of-phenol-via-meta-cleavage-pathway-triggers-de-novo-tag-biosynthesis-pathway-in-oleaginous-yeast
#3
Alok Patel, Km Sartaj, Neha Arora, Vikas Pruthi, Parul A Pruthi
Phenol is reported to be one of the most toxic environmental pollutants present in the discharge of various industrial effluents causing a serious threat to the existing biome. Biodegradation of phenol by oleaginous yeast Rhodosporidium kratochvilovae HIMPA1 was found to degrade 1000mg/l phenol. The pathways for phenol degradation by both ortho and meta-cleavage were proposed by the identification of metabolites and enzymatic assays of ring cleavage enzymes in the cell extracts. Results suggest that this oleaginous yeast degrade phenol via meta-cleavage pathway and accumulates a high quantity of lipid content (64...
July 6, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28706285/transcriptional-regulation-of-cellulose-biosynthesis-during-the-early-phase-of-nitrogen-deprivation-in-nannochloropsis-salina
#4
Seok Won Jeong, Seung Won Nam, Kwon HwangBo, Won Joong Jeong, Byeong-Ryool Jeong, Yong Keun Chang, Youn-Il Park
Microalgal photosynthesis provides energy and carbon-containing precursors for the biosynthesis of storage carbohydrates such as starch, chrysolaminarin, lipids, and cell wall components. Under mild nitrogen deficiency (N-), some Nannochloropsis species accumulate lipid by augmenting cytosolic fatty acid biosynthesis with a temporary increase in laminarin. Accordingly, biosynthesis of the cellulose-rich cell wall should change in response to N- stress because this biosynthetic pathway begins with utilisation of the hexose phosphate pool supplied from photosynthesis...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694845/a-type-i-diacylglycerol-acyltransferase-modulates-triacylglycerol-biosynthesis-and-fatty-acid-composition-in-the-oleaginous-microalga-nannochloropsis-oceanica
#5
Hehong Wei, Ying Shi, Xiaonian Ma, Yufang Pan, Hanhua Hu, Yantao Li, Ming Luo, Henri Gerken, Jin Liu
BACKGROUND: Photosynthetic oleaginous microalgae are considered promising feedstocks for biofuels. The marine microalga, Nannochloropsis oceanica, has been attracting ever-increasing interest because of its fast growth, high triacylglycerol (TAG) content, and available genome sequence and genetic tools. Diacylglycerol acyltransferase (DGAT) catalyzes the last and committed step of TAG biosynthesis in the acyl-CoA-dependent pathway. Previous studies have identified 13 putative DGAT-encoding genes in the genome of N...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28684180/enhancing-linalool-production-by-engineering-oleaginous-yeast-yarrowia-lipolytica
#6
Xuan Cao, Liu-Jing Wei, Jia-Yu Lin, Qiang Hua
In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20(F88W-N119W) gene resulted in further linalool production...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28681845/bacterial-lipid-droplets-bind-to-dna-via-an-intermediary-protein-that-enhances-survival-under-stress
#7
Congyan Zhang, Li Yang, Yunfeng Ding, Yang Wang, Lan Lan, Qin Ma, Xiang Chi, Peng Wei, Yongfang Zhao, Alexander Steinbüchel, Hong Zhang, Pingsheng Liu
Lipid droplets (LDs) are multi-functional organelles consisting of a neutral lipid core surrounded by a phospholipid monolayer, and exist in organisms ranging from bacteria to humans. Here we study the functions of LDs in the oleaginous bacterium Rhodococcus jostii. We show that these LDs bind to genomic DNA through the major LD protein, MLDS, which increases survival rate of the bacterial cells under nutritional and genotoxic stress. MLDS expression is regulated by a transcriptional regulator, MLDSR, that binds to the operator and promoter of the operon encoding both proteins...
July 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/28673516/metabolic-engineering-of-oleaginous-fungus-mortierella-alpina-for-high-production-of-oleic-and-linoleic-acids
#8
Takaiku Sakamoto, Eiji Sakuradani, Tomoyo Okuda, Hiroshi Kikukawa, Akinori Ando, Shigenobu Kishino, Yoshihiro Izumi, Takeshi Bamba, Jun Shima, Jun Ogawa
The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68...
June 19, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28666148/corn-bran-bioprocessing-development-of-an-integrated-process-for-microbial-lipids-production
#9
Jung-Eun Lee, Praveen V Vadlani, Jon Faubion
This study investigated the potential of corn bran as a feedstock for microbial lipid production using oleaginous yeast, Trichosporon oleaginosus ATCC20509. Different conditions (solid loading of biomass, acid loading, and pretreatment duration) were applied to optimize pretreatment processes using the Box-Behnken design. The highest sugar yield of 0.53g/g was obtained from corn bran hydrolysates at a pretreatment condition of 5% solid loading and 1% acid loading for 30min. Compared with synthetic media, up to 50% higher lipid accumulations in T...
June 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28665433/fast-microwave-assisted-acidolysis-a-new-biorefinery-approach-for-the-zero-waste-utilisation-of-lignocellulosic-biomass-to-produce-high-quality-lignin-and-fermentable-saccharides
#10
Long Zhou, Fabio Santomauro, Jiajun Fan, Duncan Macquarrie, James Clark, Christopher J Chuck, Vitaliy Budarin
Generally, biorefineries convert lignocellulosic biomass into a range of biofuels and further value added chemicals. However, conventional biorefinery processes focus mainly on the cellulose and hemicellulose fractions and therefore produce only low quality lignin, which is commonly burnt to provide process heat. To make full use of the biomass, more attention needs to be focused on novel separation techniques, where high quality lignin can be isolated that is suitable for further valorisation into aromatic chemicals and fuel components...
June 30, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28649761/functional-assessment-of-plant-and-microalgal-lipid-pathway-genes-in-yeast-to-enhance-microbial-industrial-oil-production
#11
Huadong Peng, Lalehvash Moghaddam, Anthony Brinin, Brett Williams, Sagadevan Mundree, Victoria S Haritos
As promising alternatives to fossil-derived oils, microbial lipids are important as industrial feedstocks for biofuels and oleochemicals. Our broad aim is to increase lipid content in oleaginous yeast through expression of lipid accumulation genes and use Saccharomyces cerevisiae to functionally assess genes obtained from oil-producing plants and microalgae. Lipid accumulation genes DGAT (diacylglycerol acyltransferase), PDAT (phospholipid: diacylglycerol acyltransferase) and ROD1 (phosphatidylcholine: diacylglycerol choline-phosphotransferase) were separately expressed in yeast and lipid production measured by fluorescence, solvent extraction, TLC and GC of fatty acid methyl esters...
June 25, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28642053/from-low-cost-substrates-to-single-cell-oils-synthesized-by-oleaginous-yeasts
#12
REVIEW
Lei Qin, Lu Liu, An-Ping Zeng, Dong Wei
As new feedstock for biofuels, microbial oils have received worldwide attentions due to their environmentally-friendly characters. Microbial oil production based on low-cost raw materials is significantly attractive to the current biodiesel refinery industry. In terms of SCOs production, oleaginous yeast has numerous advantages over bacteria, molds and microalgae based on their high growth rate and lipid yield. Numerous efforts have been made on the competitive lipid production combining the use of cheap raw materials as substrates by yeasts...
May 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28637506/improved-%C3%AE-linolenic-acid-production-in-mucor-circinelloides-by-homologous-overexpressing-of-delta-12-and-delta-6-desaturases
#13
Yao Zhang, Xiao Luan, Huaiyuan Zhang, Victoriano Garre, Yuanda Song, Colin Ratledge
BACKGROUND: γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding of the correlation between desaturases and GLA synthesis in oleaginous fungi is incomplete. In previous work, we found that a large amount of oleic acid (OA) had not been converted to linoleic acid (LA) or GLA in Mucor circinelloides CBS 277.49, which may be due to inadequate activities of the delta-12 or delta-6 desaturases, and thus leading to the accumulation of OA and LA...
June 21, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28636483/lumping-kinetics-of-abe-fermentation-wastewater-treatment-by-oleaginous-yeast-trichosporon-cutaneum
#14
Can Wang, Chao Huang, Xue-Fang Chen, Hai-Rong Zhang, Lian Xiong, Xiao-Mei Li, Hai-Jun Guo, Gao-Xiang Qi, Xiao-Qing Lin, Xin-De Chen
Lumping kinetics models were built for the biological treatment of ABE (acetone-butanol-ethanol) fermentation wastewater by oleaginous yeast Trichosporon cutaneum with different fermentation temperatures. Compared with high temperature (33°C, 306 K) and low temperature (23°C, 296 K), medium temperature (28°C, 301 K) was beneficial for the cell growth and COD degradation during the early stage of fermentation but the final yeast biomass and COD removal were influenced little. By lumping method, the materials in the bioconversion network were divided into five lumps (COD, lipid, polysaccharide, other intracellular products, other extracellular products), and the nine rate constants (k1-k9) for the models can well explain the bioconversion laws...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28634846/lipid-accumulation-and-metabolic-analysis-based-on-transcriptome-sequencing-of-filamentous-oleaginous-microalgae-tribonema-minus-at-different-growth-phases
#15
Hui Wang, Lili Gao, Huimin Shao, Wenjun Zhou, Tianzhong Liu
Filamentous oleaginous microalgae specie Tribonema minus is a promising feedstock for biodiesel production. However, the metabolic mechanism of lipid production in this filamentous microalgal specie remains unclear. Here, we compared the lipid accumulation of T. minus at different growth phases, and described the de novo transcriptome sequencing and assembly and identified important pathways and genes involved in TAG production. Total lipid increased by 2.5-fold and its TAG level in total lipid reached 81.1% at stationary phase...
June 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28631219/agrobacterium-tumefaciens-mediated-transformation-of-oleaginous-yeast-lipomyces-species
#16
Ziyu Dai, Shuang Deng, David E Culley, Kenneth S Bruno, Jon K Magnuson
Interest in using renewable sources of carbon, especially lignocellulosic biomass, for the production of hydrocarbon fuels and chemicals has fueled interest in exploring various organisms capable of producing hydrocarbon biofuels and chemicals or their precursors. The oleaginous (oil-producing) yeast Lipomyces starkeyi is the subject of active research regarding the production of triacylglycerides as hydrocarbon fuel precursors using a variety of carbohydrate and nutrient sources. The genome of L. starkeyi has been published, which opens the door to production strain improvements through the development and use of the tools of synthetic biology for this oleaginous species...
June 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28630295/application-of-metabolic-controls-for-the-maximization-of-lipid-production-in-semicontinuous-fermentation
#17
Jingyang Xu, Nian Liu, Kangjian Qiao, Sebastian Vogg, Gregory Stephanopoulos
Acetic acid can be generated through syngas fermentation, lignocellulosic biomass degradation, and organic waste anaerobic digestion. Microbial conversion of acetate into triacylglycerols for biofuel production has many advantages, including low-cost or even negative-cost feedstock and environmental benefits. The main issue stems from the dilute nature of acetate produced in such systems, which is costly to be processed on an industrial scale. To tackle this problem, we established an efficient bioprocess for converting dilute acetate into lipids, using the oleaginous yeast Yarrowia lipolytica in a semicontinuous system...
July 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628983/immobilized-oleaginous-microalgae-for-production-of-lipid-and-phytoremediation-of-secondary-effluent-from-palm-oil-mill-in-fluidized-bed-photobioreactor
#18
Benjamas Cheirsilp, Tipawan Thawechai, Poonsuk Prasertsan
Oleaginous microalga Nannochloropsis sp. was immobilized in alginate gel beads and cultivated under optimal conditions that their growth and lipid production were comparable to those of free cells. The immobilized cells were used in phytoremediation of secondary effluent from palm oil mill and easily recovered by simple sieving method. The immobilized cells contributed to removal of nitrogen and phosphorus >90% and CO2 mitigation >99%. They also gave the biomass and lipid production of 1.300±0.050g/L and 0...
October 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28625352/rna-seq-and-metabolic-flux-analysis-of-tetraselmis-sp-m8-during-nitrogen-starvation-reveals-a-two-stage-lipid-accumulation-mechanism
#19
David K Y Lim, Holger Schuhmann, Skye R Thomas-Hall, Kenneth C K Chan, Taylor J Wass, Felipe Aguilera, T Catalina Adarme-Vega, Cristiana G O Dal'Molin, Glen J Thorpe, Jacqueline Batley, David Edwards, Peer M Schenk
To map out key lipid-related pathways that lead to rapid triacylglyceride accumulation in oleaginous microalgae, RNA-Seq was performed with Tetraselmis sp. M8 at 24h after exhaustion of exogenous nitrogen to reveal molecular changes during early stationary phase. Further gene expression profiling by quantitative real-time PCR at 16-72h revealed a distinct shift in expression of the fatty acid/triacylglyceride biosynthesis and β-oxidation pathways, when cells transitioned from log-phase into early-stationary and stationary phase...
June 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28625124/occurrence-and-importance-of-plant-lipids-a-promising-insight-into-algae
#20
Nihed Ben Halima
BACKGROUND: Lipids are biomolecules with interesting structural variability. They are involved in many processes such as the storage of energy, in nutrition and are also of relevance for signal transduction processes, in apoptosis, cell differentiation and phagocytosis, etc. Algae and microalgae are a promising and very diverse group of organisms. These living arganisms inhabit a vaste range of ecosystems from the Antarctic to the Deserts. They account for above half the primary productivity at the base of the food chain because of their multiple nutritional products including, for instance, lipids...
June 19, 2017: Recent Patents on Food, Nutrition & Agriculture
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