keyword
https://read.qxmd.com/read/37566613/matriptase-dependent-epidermal-pre-neoplasm-in-zebrafish-embryos-caused-by-a-combination-of-hypotonic-stress-and-epithelial-polarity-defects
#21
JOURNAL ARTICLE
Julia Hatzold, Verena Nett, Stephanie Brantsch, Jin-Li Zhang, Joy Armistead, Heike Wessendorf, Rebecca Stephens, Patrick O Humbert, Sandra Iden, Matthias Hammerschmidt
Aberrantly up-regulated activity of the type II transmembrane protease Matriptase-1 has been associated with the development and progression of a range of epithelial-derived carcinomas, and a variety of signaling pathways can mediate Matriptase-dependent tumorigenic events. During mammalian carcinogenesis, gain of Matriptase activity often results from imbalanced ratios between Matriptase and its cognate transmembrane inhibitor Hai1. Similarly, in zebrafish, unrestrained Matriptase activity due to loss of hai1a results in epidermal pre-neoplasms already during embryogenesis...
August 2023: PLoS Genetics
https://read.qxmd.com/read/37456981/development-of-new-approach-methods-for-the-identification-and-characterization-of-endocrine-metabolic-disruptors-a-parc-project
#22
JOURNAL ARTICLE
Albert Braeuning, Patrick Balaguer, William Bourguet, Jordi Carreras-Puigvert, Katreece Feiertag, Jorke H Kamstra, Dries Knapen, Dajana Lichtenstein, Philip Marx-Stoelting, Jonne Rietdijk, Kristin Schubert, Ola Spjuth, Evelyn Stinckens, Kathrin Thedieck, Rik van den Boom, Lucia Vergauwen, Martin von Bergen, Neele Wewer, Daniel Zalko
In past times, the analysis of endocrine disrupting properties of chemicals has mainly been focused on (anti-)estrogenic or (anti-)androgenic properties, as well as on aspects of steroidogenesis and the modulation of thyroid signaling. More recently, disruption of energy metabolism and related signaling pathways by exogenous substances, so-called metabolism-disrupting chemicals (MDCs) have come into focus. While general effects such as body and organ weight changes are routinely monitored in animal studies, there is a clear lack of mechanistic test systems to determine and characterize the metabolism-disrupting potential of chemicals...
2023: Frontiers in toxicology
https://read.qxmd.com/read/37268261/cbf%C3%AE-is-induced-by-spring-viremia-of-carp-virus-and-promotes-virus-replication-in-zebrafish
#23
JOURNAL ARTICLE
Ning Ji, Wenji Huang, Huifeng Dang, Hehe Xiao, Yanjie Shi, Jiahong Guo, Kangyong Chen, Junya Wang, Jun Zou
The core binding factor subunit beta (CBFβ) is a transcription factor that forms a complex with virial proteins to promote viral infection. In this study, we identified a CBFβ homolog from zebrafish (zfCBFβ) and characterized the biological activity. The deduced zfCBFβ protein was highly similar to orthologs from other species. The zfcbfβ gene was constitutively expressed in tissues and was induced in immune tissues after infection with spring viremia carp virus (SVCV) and stimulation with poly(I:C)...
May 31, 2023: Developmental and Comparative Immunology
https://read.qxmd.com/read/37251091/in-vivo-drug-screening-to-identify-anti-metastatic-drugs-in-twist1a-er-t2-transgenic-zebrafish
#24
JOURNAL ARTICLE
Joji Nakayama, Hideki Makinoshima, Zhiyuan Gong
Here, we present an in vivo drug screening protocol using a zebrafish model of metastasis for the identification of anti-metastatic drugs. A tamoxifen-controllable Twist1a-ERT2 transgenic zebrafish line was established to serve as a platform for the identification. By crossing Twist1a-ERT2 with xmrk (a homolog of hyperactive form of the epidermal growth factor receptor) transgenic zebrafish, which develop hepatocellular carcinoma, approximately 80% of the double transgenic zebrafish show spontaneous cell dissemination of mCherry-labeled hepatocytes from the liver to the entire abdomen and tail regions in five days, through induction of epithelial to mesenchymal transition (EMT)...
May 20, 2023: Bio-protocol
https://read.qxmd.com/read/37141637/deciphering-the-pathogenesis-of-retinopathy-associated-with-carnitine-palmitoyltransferase-i-deficiency-in-zebrafish-model
#25
JOURNAL ARTICLE
Zulvikar Syambani Ulhaq, Yukiko Ogino, William Ka Fai Tse
Fatty acid oxidation disorders (FAODs) are a group of rare genetic metabolic disorders caused by mutations in genes responsible for transporting and metabolizing fatty acids in the mitochondria. One crucial enzyme involved in this process is carnitine palmitoyltransferase I (CPT1), which transports long-chain fatty acids to the mitochondrial matrix for beta-oxidation. Defects in beta-oxidation enzymes often lead to pigmentary retinopathy; however, the underlying mechanisms are not entirely understood. To investigate FAOD and its impact on the retina, we employed zebrafish as a model organism...
July 5, 2023: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/37067038/20-s-protopanaxatriol-improves-cognitive-function-of-alzheimer-s-disease-by-promoting-endogenous-neurogenesis
#26
JOURNAL ARTICLE
Qiang Zhang, Xuehan Yang, Jingyue Liu, Jiayi Song, Siwei Zhang, Li Chen, Ming Zhang
Neurogenesis in Alzheimer's disease (AD) is impaired, and thus promoting neurogenesis is a promising strategy for treating AD-related cognition impairment. Here, we found that 20 S -protopanaxatriol (PPT) can alleviate cognitive deficits in an AlCl3 -induced AD zebrafish model in Y and T maze tests and increase the HUC expression of the telencephalon. PPT promotes neural stem cell (NSC) line differentiation into neurons under AD condition. PPT significantly alleviated the apoptosis and cell cycle arrest of NSCs induced by amyloid-beta...
April 17, 2023: Food & Function
https://read.qxmd.com/read/37062434/sulfoxaflor-induces-immunotoxicity-in-zebrafish-danio-rerio-by-activating-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway
#27
JOURNAL ARTICLE
Kexin Wang, Yong Huang, Bo Cheng, Jing Guo, Yuyang Peng, Suwen Zeng, June Zhang, Huiqiang Lu
Sulfoxaflor is an insecticide that is widely used and affects the nervous system of sucking pests. However, studies on the molecular mechanism of the toxicity of sulfoxaflor to non-target species are limited. Zebrafish (Danio rerio) was used as an experimental subject in this study. Zebrafish embryos were exposed to 20, 25, and 30 mg/L sulfoxaflor solution to detect hatchability, mortality, heart rate, neutrophil count, oxidative stress, and expression of genes related to apoptosis and immune inflammation. The results showed that zebrafish embryos exposed to sulfoxaflor solution increased mortality and growth retardation, and the number of innate immune cells decreased significantly...
April 14, 2023: Fish & Shellfish Immunology
https://read.qxmd.com/read/36975217/next-generation-plasmids-for-transgenesis-in-zebrafish-and-beyond
#28
JOURNAL ARTICLE
Cassie L Kemmler, Hannah R Moran, Brooke F Murray, Aaron Scoresby, John R Klem, Rachel L Eckert, Elizabeth Lepovsky, Sylvain Bertho, Susan Nieuwenhuize, Sibylle Burger, Gianluca D'Agati, Charles Betz, Ann-Christin Puller, Anastasia Felker, Karolina Ditrychova, Seraina Bötschi, Markus Affolter, Nicolas Rohner, C Ben Lovely, Kristen M Kwan, Alexa Burger, Christian Mosimann
Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Gateway-compatible vector collections has transformed zebrafish transgenesis with an accessible, modular system. Here, we established several next-generation transgenesis tools for zebrafish and other species to expand and enhance transgenic applications. To facilitate gene-regulatory element testing, we generated Gateway middle entry vectors harboring the small mouse beta-globin minimal promoter coupled to several fluorophores, CreERT2, and Gal4...
March 28, 2023: Development
https://read.qxmd.com/read/36817116/-in-silico-prediction-and-biological-assessment-of-novel-angiogenesis-modulators-from-traditional-chinese-medicine
#29
JOURNAL ARTICLE
Yingli Zhu, Hongbin Yang, Liwen Han, Lewis H Mervin, Layla Hosseini-Gerami, Peihai Li, Peter Wright, Maria-Anna Trapotsi, Kechun Liu, Tai-Ping Fan, Andreas Bender
Uncontrolled angiogenesis is a common denominator underlying many deadly and debilitating diseases such as myocardial infarction, chronic wounds, cancer, and age-related macular degeneration. As the current range of FDA-approved angiogenesis-based medicines are far from meeting clinical demands, the vast reserve of natural products from traditional Chinese medicine (TCM) offers an alternative source for developing pro-angiogenic or anti-angiogenic modulators. Here, we investigated 100 traditional Chinese medicine-derived individual metabolites which had reported gene expression in MCF7 cell lines in the Gene Expression Omnibus (GSE85871)...
2023: Frontiers in Pharmacology
https://read.qxmd.com/read/36765531/anti-vimentin-nanobody-decreases-glioblastoma-cell-invasion-in-vitro-and-in-vivo
#30
JOURNAL ARTICLE
Alja Zottel, Metka Novak, Neja Šamec, Bernarda Majc, Sara Colja, Mojca Katrašnik, Miloš Vittori, Barbara Hrastar, Ana Rotter, Andrej Porčnik, Tamara Lah Turnšek, Radovan Komel, Barbara Breznik, Ivana Jovčevska
PURPOSE: Glioblastoma (GBM) is the most common primary brain tumour and one of the deadliest cancers. In addition to late diagnosis and inadequate treatment, the extremely low survival rate is also due to the lack of appropriate therapeutic biomarkers and corresponding therapeutic agents. One of the potential therapeutic biomarkers is the intermediate filament vimentin, which is associated with epithelial-mesenchymal transition (EMT). The purpose of this study was to analyse the effect of the anti-vimentin nanobody Nb79 on cell invasion in vitro and in vivo...
January 17, 2023: Cancers
https://read.qxmd.com/read/36633628/loss-of-rreb1-in-pancreatic-beta-cells-reduces-cellular-insulin-content-and-affects-endocrine-cell-gene-expression
#31
JOURNAL ARTICLE
Katia K Mattis, Nicole A J Krentz, Christoph Metzendorf, Fernando Abaitua, Aliya F Spigelman, Han Sun, Jennifer M Ikle, Swaraj Thaman, Antje K Rottner, Austin Bautista, Eugenia Mazzaferro, Marta Perez-Alcantara, Jocelyn E Manning Fox, Jason M Torres, Agata Wesolowska-Andersen, Grace Z Yu, Anubha Mahajan, Anders Larsson, Patrick E MacDonald, Benjamin Davies, Marcel den Hoed, Anna L Gloyn
AIMS/HYPOTHESIS: Genome-wide studies have uncovered multiple independent signals at the RREB1 locus associated with altered type 2 diabetes risk and related glycaemic traits. However, little is known about the function of the zinc finger transcription factor Ras-responsive element binding protein 1 (RREB1) in glucose homeostasis or how changes in its expression and/or function influence diabetes risk. METHODS: A zebrafish model lacking rreb1a and rreb1b was used to study the effect of RREB1 loss in vivo...
January 12, 2023: Diabetologia
https://read.qxmd.com/read/36567454/salidroside-alleviates-dexamethasone-induced-inhibition-of-bone-formation-via-transforming-growth-factor-beta-smad2-3-signaling-pathway
#32
JOURNAL ARTICLE
Baocheng Xie, Huan Zhou, Hongyu Liu, Shiyi Liao, Chenhui Zhou, Daohua Xu
Glucocorticoid-induced osteoporosis is the third epidemic osteoporosis following postmenopausal and senileosteoporosis. According to one study, salidroside made ovariectomized rats' bones strong. Salidroside's potential for treating glucocorticoid-induced osteoporosis remains unproven. This study aimed to investigate the protective effect and mechanism of salidroside on dexamethasone-induced osteogenic differentiation and bone formation in MC3T3-E1 cells and zebrafish. The study proved that salindroside had no harmful impact on MC3T3E1 cells...
December 25, 2022: Phytotherapy Research: PTR
https://read.qxmd.com/read/36499397/glomerular-endothelial-cell-derived-mir-200c-impairs-glomerular-homeostasis-by-targeting-podocyte-vegf-a
#33
JOURNAL ARTICLE
Raluca Ursu, Nina Sopel, Alexandra Ohs, Ramesh Tati, Lisa Buvall, Jenny Nyström, Mario Schiffer, Janina Müller-Deile
Deciphering the pathophysiological mechanisms of primary podocytopathies that can lead to end-stage renal disease and increased mortality is an unmet need. Studying how microRNAs (miRs) interfere with various signaling pathways enables identification of pathomechanisms, novel biomarkers and potential therapeutic options. We investigated the expression of miR-200c in urine from patients with different renal diseases as a potential candidate involved in podocytopathies. The role of miR-200c for the glomerulus and its potential targets were studied in cultured human podocytes, human glomerular endothelial cells and in the zebrafish model...
December 1, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/36456994/a-synthetic-pregnenolone-analog-promotes-microtubule-dynamics-and-neural-development
#34
JOURNAL ARTICLE
Viktoryia Kolas, Jose Sandino A Bandonil, Niaz Wali, Kuo-Chiang Hsia, Jiun-Jie Shie, Bon-Chu Chung
BACKGROUND: Pregnenolone (P5) is a neurosteroid that promotes microtubule polymerization. It also reduces stress and negative symptoms of schizophrenia, promotes memory, as well as recovery from spinal cord injury. P5 is the first substance in the steroid-synthetic pathway; it can be further metabolized into other steroids. Therefore, it is difficult to differentiate the roles of P5 versus its metabolites in the brain. To alleviate this problem, we synthesized and screened a series of non-metabolizable P5 derivatives for their ability to polymerize microtubules similar to P5...
December 1, 2022: Cell & Bioscience
https://read.qxmd.com/read/36418055/kras-mutation-induced-endmt-of-brain-arteriovenous-malformation-is-mediated-through-the-tgf-%C3%AE-bmp-smad4-pathway
#35
JOURNAL ARTICLE
Hongyuan Xu, Ran Huo, Hao Li, Yuming Jiao, Jiancong Weng, Jie Wang, Zihan Yan, Junze Zhang, Shaozhi Zhao, Qiheng He, Yingfan Sun, Shuo Wang, Yong Cao
OBJECTIVE: Somatic KRAS mutations have been identified in the majority of brain arteriovenous malformations (bAVMs), and subsequent in vivo experiments have confirmed that KRAS mutation in endothelial cells (ECs) causes AVMs in mouse and zebrafish models. Our previous study demonstrated that the KRASG12D mutant independently induced the endothelial-mesenchymal transition (EndMT), which was reversed by treatment with the lipid-lowering drug lovastatin. However, the underlying mechanisms of action were unclear...
November 23, 2022: Stroke and Vascular Neurology
https://read.qxmd.com/read/36410639/protective-effects-of-cyanidin-3-o-glucoside-on-bpa-induced-neurodevelopmental-toxicity-in-zebrafish-embryo-model
#36
JOURNAL ARTICLE
Guangchao Yang, Lipin Yang, Qin Liu, Zhenzhu Zhu, Qian Yang, Jining Liu, Trust Beta
Bisphenol A (BPA) is ubiquitous in the environment and poses a threat to wildlife and human health. It has been reported that BPA may cause the neurotoxicity during gestational and neonatal periods. Cyanidin-3-O-glucoside (C3G) is one of the most abundant anthocyanins that has shown multiple bio-functions. In this study, the protective effects and possible mechanism of C3G against BPA-induced neurodevelopment toxicity in zebrafish embryos/larvae were studied. The results showed that co-exposure of C3G (25 μg/mL) significantly attenuated BPA-induced deficit in locomotor behavior and restored the BPA-induced aberrant changes in brain morphology of zebrafish larvae...
November 18, 2022: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://read.qxmd.com/read/36405758/the-effect-and-underlying-mechanism-of-yeast-%C3%AE-glucan-on-antiviral-resistance-of-zebrafish-against-spring-viremia-of-carp-virus-infection
#37
JOURNAL ARTICLE
Hui Liang, Yu Li, Ming Li, Wei Zhou, Jie Chen, Zhen Zhang, Yalin Yang, Chao Ran, Zhigang Zhou
β-glucan has been used as immunostimulant for fish. However, the effect of yeast β-glucan on viral infections has been less studied in fish. In this study, we investigated the effects of β-glucan on the resistance of zebrafish against spring viraemia of carp virus (SVCV) and elucidated the underlying mechanisms. Zebrafish were fed with a control diet or diet supplemented with 0.01% and 0.025% β-glucan for 2 weeks, and were challenged by SVCV. Zebrafish embryonic fibroblast (ZF4) cells were treated with 5 μg/mL β-glucan and were infected by SVCV...
2022: Frontiers in Immunology
https://read.qxmd.com/read/36372285/nox4-promotes-osteoblast-differentiation-through-tgf-beta-signal-pathway
#38
JOURNAL ARTICLE
Zihou Cao, Gongwen Liu, Hui Zhang, Mingyong Wang, Youjia Xu
NADPH oxidase 4 (Nox4) is the main source of reactive oxygen species, which promote osteoclast formation and lead to bone loss, thereby causing osteoporosis. However, the role of Nox4 in osteoblasts during early development remains unclear. We used zebrafish to study the effect of Nox4 deletion on bone mineralization in early development. nox4-/- zebrafish showed decreased bone mineralization during early development and significantly reduced numbers of osteoblasts, osteoclasts, and chondrocytes. Transcriptome sequencing showed that the TGF-β signaling pathway was significantly disrupted in nox4-/- zebrafish...
November 10, 2022: Free Radical Biology & Medicine
https://read.qxmd.com/read/36314827/irf2-cooperates-with-phosphoprotein-of-spring-viremia-of-carp-virus-to-suppress-antiviral-response-in-zebrafish
#39
JOURNAL ARTICLE
Wenji Huang, Xin Zhao, Ning Ji, Jiahong Guo, Jianhua Feng, Kangyong Chen, Yaxin Wu, Junya Wang, Hao Feng, Jun Zou
IFN regulatory factor (IRF) 2 belongs to the IRF1 subfamily, and its functions are not yet fully understood. In this study, we showed that IRF2a was a negative regulator of the interferon (IFN) response induced by spring viremia of carp virus (SVCV). Irf2a-/- knockout zebrafish were less susceptible to SVCV than wild-type fish. Transcriptomic analysis reveals that differentially expressed genes (DEGs) in the irf2a-/- and irf2a+/+ cells derived caudal fins were mainly involved in cytokine-cytokine receptor interaction, mitogen-activated protein kinase (MAPK) signaling pathway, and transforming growth factor-beta (TGF-beta) signaling pathway...
October 31, 2022: Journal of Virology
https://read.qxmd.com/read/36247002/oxytocin-promotes-epicardial-cell-activation-and-heart-regeneration-after-cardiac-injury
#40
JOURNAL ARTICLE
Aaron H Wasserman, Amanda R Huang, Yonatan R Lewis-Israeli, McKenna D Dooley, Allison L Mitchell, Manigandan Venkatesan, Aitor Aguirre
Cardiovascular disease (CVD) is one of the leading causes of mortality worldwide, and frequently leads to massive heart injury and the loss of billions of cardiac muscle cells and associated vasculature. Critical work in the last 2 decades demonstrated that these lost cells can be partially regenerated by the epicardium, the outermost mesothelial layer of the heart, in a process that highly recapitulates its role in heart development. Upon cardiac injury, mature epicardial cells activate and undergo an epithelial-mesenchymal transition (EMT) to form epicardium-derived progenitor cells (EpiPCs), multipotent progenitors that can differentiate into several important cardiac lineages, including cardiomyocytes and vascular cells...
2022: Frontiers in Cell and Developmental Biology
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