Cassie L Kemmler, Hannah R Moran, Brooke F Murray, Aaron Scoresby, John R Klem, Rachel L Eckert, Elizabeth Lepovsky, Sylvain Bertho, Susan Nieuwenhuize, Sibylle Burger, Gianluca D'Agati, Charles Betz, Ann-Christin Puller, Anastasia Felker, Karolina Ditrychova, Seraina Bötschi, Markus Affolter, Nicolas Rohner, C Ben Lovely, Kristen M Kwan, Alexa Burger, Christian Mosimann
Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Gateway-compatible vector collections has transformed zebrafish transgenesis with an accessible, modular system. Here, we established several next-generation transgenesis tools for zebrafish and other species to expand and enhance transgenic applications. To facilitate gene-regulatory element testing, we generated Gateway middle entry vectors harboring the small mouse beta-globin minimal promoter coupled to several fluorophores, CreERT2, and Gal4...
March 28, 2023: Development