Verena Haage, John F Tuddenham, Natacha Comandante-Lou, Alex Bautista, Anna Monzel, Rebecca Chiu, Masashi Fujita, Frankie G Garcia, Prabesh Bhattarai, Ronak Patel, Alice Buonfiglioli, Juan Idiarte, Mathieu Herman, Alison Rinderspacher, Angeliki Mela, Wenting Zhao, Michael G Argenziano, Julia L Furnari, Matei A Banu, Donald W Landry, Jeffrey N Bruce, Peter Canoll, Ya Zhang, Tal Nuriel, Caghan Kizil, Andrew A Sproul, Lotje D de Witte, Peter A Sims, Vilas Menon, Martin Picard, Philip L De Jager
While efforts to identify microglial subtypes have recently accelerated, the relation of transcriptomically defined states to function has been largely limited to in silico annotations. Here, we characterize a set of pharmacological compounds that have been proposed to polarize human microglia towards two distinct states - one enriched for AD and MS genes and another characterized by increased expression of antigen presentation genes. Using different model systems including HMC3 cells, iPSC-derived microglia and cerebral organoids, we characterize the effect of these compounds in mimicking human microglial subtypes in vitro ...
February 6, 2024: bioRxiv