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Zebrafish beta cell

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https://www.readbyqxmd.com/read/29777274/the-natural-agent-4-vinylphenol-targets-metastasis-and-stemness-features-in-breast-cancer-stem-like-cells
#1
Hoi-Wing Leung, Chun-Hay Ko, Grace Gar-Lee Yue, Ingrid Herr, Clara Bik-San Lau
BACKGROUND AND PURPOSE: Cancer stem-like cells (CSC) are regarded as the source of tumour origins, metastasis and drug resistance, and limits current treatment regimens. Previously, we reported the first study of the anti-angiogenic and anti-tumour activities of 4-vinylphenol. To further examine the therapeutic role of 4-vinylphenol, the inhibitory effects of 4-vinylphenol on cancer stemness, drug resistance and metastasis in breast cancer were investigated in the present study. STUDY DESIGN AND METHODS: We enriched parental MDA-MB-231 cells with CSCs in serum-free medium to give spheroids...
May 18, 2018: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/29732682/structure-dissection-of-zebrafish-progranulins-identifies-a-well-folded-granulin-epithelin-module-protein-with-pro-cell-survival-activities
#2
Ping Wang, Babykumari Chitramuthu, Andrew Bateman, Hugh P J Bennett, Ping Xu, Feng Ni
The ancient and pluripotent progranulins contain multiple repeats of a cysteine-rich sequence motif of ∼60 amino acids, called the granulin/epithelin module (GEM) with a prototypic structure of four β-hairpins zipped together by six inter-hairpin disulfide bonds. Prevalence of this disulfide-enforced structure is assessed here by an expression screening of 19 unique GEM sequences of the four progranulins in the zebrafish genome, progranulins 1, 2, A and B. While a majority of the expressed GEM peptides did not exhibit uniquely-folded conformations, module AaE from progranulin A and AbB from progranulin B were found to fold into the protopypic 4-hairpin structure along with disulfide formation...
May 7, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29663654/nifurpirinol-a-more-potent-and-reliable-substrate-compared-to-metronidazole-for-nitroreductase-mediated-cell-ablations
#3
David Bergemann, Laura Massoz, Jordane Bourdouxhe, Claudio A Carril Pardo, Marianne L Voz, Bernard Peers, Isabelle Manfroid
The zebrafish is a popular animal model with well-known regenerative capabilities. To study regeneration in this fish, the nitroreductase/metronidazole-mediated system is widely used for targeted ablation of various cell types. Nevertheless, we highlight here some variability in ablation efficiencies with the metronidazole prodrug that led us to search for a more efficient and reliable compound. Herein, we present nifurpirinol, another nitroaromatic antibiotic, as a more potent prodrug compared to metronidazole to trigger cell-ablation in nitroreductase expressing transgenic models...
April 17, 2018: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29655639/glia-maturation-factor-beta-is-required-for-reactive-gliosis-after-traumatic-brain-injury-in-zebrafish
#4
Guo Yin, Mingjun Du, Rong Li, Ke Li, Xiaomin Huang, Dongbei Duan, Xiaolan Ai, Fang Yao, Lanlan Zhang, Ziyou Hu, Bingyi Wu
Gliosis is a hallmark of neural pathology that occurs after most forms of central nervous system (CNS) injuries including traumatic brain injury (TBI). Identification of genes that control gliosis may provide novel treatment targets for patients with diverse CNS injuries. Glia maturation factor beta (GMFB) is crucial in brain development and stress response. In the present study, GMFB was found to be widely expressed in adult zebrafish telencephalon. A gmfb mutant zebrafish was created using CRISPR/cas9. In the uninjured zebrafish telencephalon, glial fibrillary acidic protein (GFAP) fibers in gmfb mutants were disorganized and shorter than wild type zebrafish...
July 2018: Experimental Neurology
https://www.readbyqxmd.com/read/29624168/age-related-islet-inflammation-marks-the-proliferative-decline-of-pancreatic-beta-cells-in-zebrafish
#5
Sharan Janjuha, Sumeet Pal Singh, Anastasia Tsakmaki, S Neda Mousavy Gharavy, Priyanka Murawala, Judith Konantz, Sarah Birke, David J Hodson, Guy A Rutter, Gavin A Bewick, Nikolay Ninov
The pancreatic islet, a cellular community harboring the insulin-producing beta-cells, is known to undergo age-related alterations. However, only a handful of signals associated with aging have been identified. By comparing beta-cells from younger and older zebrafish, here we show that the aging islets exhibit signs of chronic inflammation. These include recruitment of tnfα -expressing macrophages and the activation of NF-kB signaling in beta-cells. Using a transgenic reporter, we show that NF-kB activity is undetectable in juvenile beta-cells, whereas cells from older fish exhibit heterogeneous NF-kB activity...
April 6, 2018: ELife
https://www.readbyqxmd.com/read/29565969/the-rna-binding-protein-celf1-post-transcriptionally-regulates-p27kip1-and-dnase2b-to-control-fiber-cell-nuclear-degradation-in-lens-development
#6
Archana D Siddam, Carole Gautier-Courteille, Linette Perez-Campos, Deepti Anand, Atul Kakrana, Christine A Dang, Vincent Legagneux, Agnès Méreau, Justine Viet, Jeffrey M Gross, Luc Paillard, Salil A Lachke
Opacification of the ocular lens, termed cataract, is a common cause of blindness. To become transparent, lens fiber cells undergo degradation of their organelles, including their nuclei, presenting a fundamental question: does signaling/transcription sufficiently explain differentiation of cells progressing toward compromised transcriptional potential? We report that a conserved RNA-binding protein Celf1 post-transcriptionally controls key genes to regulate lens fiber cell differentiation. Celf1-targeted knockout mice and celf1-knockdown zebrafish and Xenopus morphants have severe eye defects/cataract...
March 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29524446/beta-glucan-enhances-the-response-to-svcv-infection-in-zebrafish
#7
Regla M Medina-Gali, María Del Mar Ortega-Villaizan, Luis Mercado, Beatriz Novoa, Julio Coll, Luis Perez
The antiviral effects of beta-glucan, an immunostimulatory agent were studied in zebrafish both in vitro and in vivo. Here we show that zebrafish ZF4 cells as well as whole fish primed with yeast β-glucan zymosan exhibited increased cytokine expression and elevated response to spring viremia of carp virus (SVCV) infection. In vitro, previous treatment of β-glucan enhanced ZF4 cell viability against SVCV infection which is associated to the activation of interferon signaling pathway and inflammatory cytokines gene expression...
July 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29516647/pancreatic-beta-cells-are-a-sensitive-target-of-embryonic-exposure-to-butylparaben-in-zebrafish-danio-rerio
#8
Sarah E Brown, Karilyn E Sant, Shana M Fleischman, Olivia Venezia, Monika A Roy, Ling Zhao, Alicia R Timme-Laragy
BACKGROUND: Butylparaben (butyl p-hydroxybenzoic acid) is a common cosmetic and pharmaceutical preservative reported to induce oxidative stress and endocrine disruption. Embryonic development is sensitive to oxidative stress, with redox potentials playing critical roles in progenitor cell fate decisions. Because pancreatic beta cells have been reported to have low antioxidant gene expression, they may be sensitive targets of oxidative stress. We tested the hypotheses that butylparaben causes oxidative stress in the developing embryo, and that pancreatic beta cells are a sensitive target of butylparaben embryotoxicity...
March 8, 2018: Birth Defects Research
https://www.readbyqxmd.com/read/29504607/the-long-non-coding-rna-uc-4-influences-cell-differentiation-through-the-tgf-beta-signaling-pathway
#9
Zijie Cheng, Qijun Zhang, Anwen Yin, Mengwen Feng, Hua Li, Hailang Liu, Yun Li, Lingmei Qian
In a previous study, we screened thousands of long non-coding RNAs (lncRNAs) to assess their potential relationship with congenital heart disease (CHD). In this study, uc.4 attracted our attention because of its high level of evolutionary conservation and its antisense orientation to the CASZ1 gene, which is vital for heart development. We explored the function of uc.4 in cells and in zebrafish, and describe a potential mechanism of action. P19 cells were used to investigate the function of uc.4. We studied the effect of uc...
February 16, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29499231/elucidation-of-possible-molecular-mechanisms-underlying-the-estrogen-induced-disruption-of-cartilage-development-in-zebrafish-larvae
#10
Hanliang He, Chunqing Wang, Qifeng Tang, Fan Yang, Youjia Xu
Estrogen can affect the cartilage development of zebrafish; however, the mechanism underlying its effects is not completely understood. Four-day-old zebrafish larvae were treated with 0.8 μM estrogen, the 5 days post fertilization (dpf) zebrafish larvae did not demonstrate obvious abnormalities during development; however, the 6 dpf and 7 dpf larvae exhibited abnormal craniofacial bone development along with craniofacial bone degradation. RNA deep sequencing was performed to elucidate the mechanism involved...
June 1, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29386244/-in-vivo-imaging-of-emerging-endocrine-cells-reveals-a-requirement-for-pi3k-regulated-motility-in-pancreatic-islet-morphogenesis
#11
Julia Freudenblum, José A Iglesias, Martin Hermann, Tanja Walsen, Armin Wilfinger, Dirk Meyer, Robin A Kimmel
The three-dimensional architecture of the pancreatic islet is integral to beta cell function, but the process of islet formation remains poorly understood due to the difficulties of imaging internal organs with cellular resolution. Within transparent zebrafish larvae, the developing pancreas is relatively superficial and thus amenable to live imaging approaches. We performed in vivo time-lapse and longitudinal imaging studies to follow islet development, visualizing both naturally occurring islet cells and cells arising with an accelerated timecourse following an induction approach...
February 8, 2018: Development
https://www.readbyqxmd.com/read/29274062/metformin-beta-cell-development-and-novel-processes-following-beta-cell-ablation-in-zebrafish
#12
Georgia Wyett, Yann Gibert, Megan Ellis, Hozana A Castillo, Jan Kaslin, Kathryn Aston-Mourney
PURPOSE: Type 1 and 2 diabetes are characterized by a loss of insulin-producing beta-cells. Current treatments help maintain blood glucose levels but cannot provide a cure. As such, a vital target for the cure of diabetes is a way to restore beta-cell mass. The drug metformin can protect cultured beta-cells/islets from hyperglycemia-induced dysfunction and death. Further, treatment of pregnant mice with metformin results in an enhanced beta-cell fraction in the embryos; however, whether this occurs via a direct effect is unknown...
February 2018: Endocrine
https://www.readbyqxmd.com/read/29140251/vg1-nodal-heterodimers-are-the-endogenous-inducers-of-mesendoderm
#13
Tessa G Montague, Alexander F Schier
Nodal is considered the key inducer of mesendoderm in vertebrate embryos and embryonic stem cells. Other TGF-beta-related signals, such as Vg1/Dvr1/Gdf3, have also been implicated in this process but their roles have been unclear or controversial. Here we report that zebrafish embryos without maternally provided vg1 fail to form endoderm and head and trunk mesoderm, and closely resemble nodal loss-of-function mutants. Although Nodal is processed and secreted without Vg1, it requires Vg1 for its endogenous activity...
November 15, 2017: ELife
https://www.readbyqxmd.com/read/29065151/g-protein-coupled-estrogen-receptor-regulates-embryonic-heart-rate-in-zebrafish
#14
Shannon N Romano, Hailey E Edwards, Jaclyn Paige Souder, Kevin J Ryan, Xiangqin Cui, Daniel A Gorelick
Estrogens act by binding to estrogen receptors alpha and beta (ERα, ERβ), ligand-dependent transcription factors that play crucial roles in sex differentiation, tumor growth and cardiovascular physiology. Estrogens also activate the G protein-coupled estrogen receptor (GPER), however the function of GPER in vivo is less well understood. Here we find that GPER is required for normal heart rate in zebrafish embryos. Acute exposure to estrogens increased heart rate in wildtype and in ERα and ERβ mutant embryos but not in GPER mutants...
October 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29044715/bayesian-genome-wide-association-analysis-for-body-weight-in-farmed-atlantic-salmon-salmo-salar-l
#15
G M Yoshida, J P Lhorente, R Carvalheiro, J M Yáñez
We performed a genome-wide association study to detect markers associated with growth traits in Atlantic salmon. The analyzed traits included body weight at tagging (BWT) and body weight at 25 months (BW25M). Genotypes of 4662 animals were imputed from the 50K SNP chip to the 200K SNP chip using fimpute software. The markers were simultaneously modeled using Bayes C to identify genomic regions associated with the traits. We identified windows explaining a maximum of 3.71% and 3.61% of the genetic variance for BWT and BW25M respectively...
December 2017: Animal Genetics
https://www.readbyqxmd.com/read/28939870/different-developmental-histories-of-beta-cells-generate-functional-and-proliferative-heterogeneity-during-islet-growth
#16
Sumeet Pal Singh, Sharan Janjuha, Theresa Hartmann, Özge Kayisoglu, Judith Konantz, Sarah Birke, Priyanka Murawala, Ezzaldin Ahmed Alfar, Kei Murata, Anne Eugster, Naoki Tsuji, Edward R Morrissey, Michael Brand, Nikolay Ninov
The proliferative and functional heterogeneity among seemingly uniform cells is a universal phenomenon. Identifying the underlying factors requires single-cell analysis of function and proliferation. Here we show that the pancreatic beta-cells in zebrafish exhibit different growth-promoting and functional properties, which in part reflect differences in the time elapsed since birth of the cells. Calcium imaging shows that the beta-cells in the embryonic islet become functional during early zebrafish development...
September 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28817646/genetic-dissection-of-endothelial-transcriptional-activity-of-zebrafish-aryl-hydrocarbon-receptors-ahrs
#17
Wade W Sugden, Roberto C Leonardo-Mendonça, Darío Acuña-Castroviejo, Arndt F Siekmann
The aryl hydrocarbon receptor (AHR) is a basic helix-loop-helix transcription factor conserved across phyla from flies to humans. Activated by a number of endogenous ligands and environmental toxins, studies on AHR function and gene regulation have largely focused on a toxicological perspective relating to aromatic hydrocarbons generated by human activities and the often-deleterious effects of exposure on vertebrates mediated by AHR activation. A growing body of work has highlighted the importance of AHR in physiologic processes, including immune cell differentiation and vascular patterning...
2017: PloS One
https://www.readbyqxmd.com/read/28636676/mitochondrion-to-endoplasmic-reticulum-apposition-length-in-zebrafish-embryo-spinal-progenitors-is-unchanged-in-response-to-perturbations-associated-with-alzheimer-s-disease
#18
Morgan Newman, Lena Halter, Anne Lim, Michael Lardelli
Mutations in the human genes PRESENILIN1 (PSEN1), PRESENILIN2 (PSEN2) and AMYLOID BETA A4 PRECURSOR PROTEIN (APP) have been identified in familial Alzheimer's disease (AD). The length of mitochondrion-endoplasmic reticulum (M-ER) appositions is increased in Psen1-/-/Psen2-/- double knockout murine embryonic fibroblasts and in fibroblasts from AD-affected individuals. Development of an easily accessible, genetically manipulable, in vivo system for studying M-ER appositions would be valuable so we attempted to manipulate M-ER apposition length in zebrafish (Danio rerio) embryos...
2017: PloS One
https://www.readbyqxmd.com/read/28559136/intradiencephalon-injection-of-histamine-inhibited-the-recovery-of-locomotor-function-of-spinal-cord-injured-zebrafish
#19
Shu-Bing Huang, Hou-De Zhao, Lin-Fang Wang, Meng-Fei Sun, Ying-Li Zhu, Yi-Bo Wu, Yi-Da Xu, Shi-Xiao Peng, Chun Cui, Yan-Qin Shen
Human spinal cord injury (SCI) usually causes irreversible disability beneath the injured site due to poor neural regeneration. On the contrary, zebrafish show significant regenerative ability after SCI, thus is usually worked as an animal model for studying neuroregeneration. Most of the previous SCI studies focused on the local site of SCI, the supraspinal-derived signals were rarely mentioned. Here we showed that intradiencephalon injection of histamine (HA) inhibited the locomotor recovery in adult zebrafish post-SCI...
July 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28530658/endoglin-controls-blood-vessel-diameter-through-endothelial-cell-shape-changes-in-response-to-haemodynamic-cues
#20
Wade W Sugden, Robert Meissner, Tinri Aegerter-Wilmsen, Roman Tsaryk, Elvin V Leonard, Jeroen Bussmann, Mailin J Hamm, Wiebke Herzog, Yi Jin, Lars Jakobsson, Cornelia Denz, Arndt F Siekmann
The hierarchical organization of properly sized blood vessels ensures the correct distribution of blood to all organs of the body, and is controlled via haemodynamic cues. In current concepts, an endothelium-dependent shear stress set point causes blood vessel enlargement in response to higher flow rates, while lower flow would lead to blood vessel narrowing, thereby establishing homeostasis. We show that during zebrafish embryonic development increases in flow, after an initial expansion of blood vessel diameters, eventually lead to vessel contraction...
June 2017: Nature Cell Biology
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