keyword
MENU ▼
Read by QxMD icon Read
search

Lpmo

keyword
https://www.readbyqxmd.com/read/28535872/fungal-secretomics-to-probe-the-biological-functions-of-lytic-polysaccharide-monooxygenases
#1
Jean-Guy Berrin, Marie-Noëlle Rosso, Maher Abou Hachem
Enzymatic degradation of plant biomass is of growing interest for the development of a sustainable bio-based industry. Filamentous fungi, which degrade complex and recalcitrant plant polymers, are proficient secretors of enzymes acting on the lignocellulose composite of plant cell walls in addition to starch, the main carbon storage reservoir. In this review, we focus on the identification of lytic polysaccharide monooxygenases (LPMOs) and their redox partners in fungal secretomes to highlight the biological functions of these remarkable enzyme systems and we discuss future trends related to LPMO-potentiated bioconversion...
May 17, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28515785/recombinant-expression-of-thermostable-processive-mteg5-endoglucanase-and-its-synergism-with-mtlpmo-from-myceliophthora-thermophila-during-the-hydrolysis-of-lignocellulosic-substrates
#2
Anthi Karnaouri, Madhu Nair Muraleedharan, Maria Dimarogona, Evangelos Topakas, Ulrika Rova, Mats Sandgren, Paul Christakopoulos
BACKGROUND: Filamentous fungi are among the most powerful cellulolytic organisms in terrestrial ecosystems. To perform the degradation of lignocellulosic substrates, these microorganisms employ both hydrolytic and oxidative mechanisms that involve the secretion and synergism of a wide variety of enzymes. Interactions between these enzymes occur on the level of saccharification, i.e., the release of neutral and oxidized products, but sometimes also reflected in the substrate liquefaction...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28491137/boosting-lpmo-driven-lignocellulose-degradation-by-polyphenol-oxidase-activated-lignin-building-blocks
#3
Matthias Frommhagen, Sumanth Kumar Mutte, Adrie H Westphal, Martijn J Koetsier, Sandra W A Hinz, Jaap Visser, Jean-Paul Vincken, Dolf Weijers, Willem J H van Berkel, Harry Gruppen, Mirjam A Kabel
BACKGROUND: Many fungi boost the deconstruction of lignocellulosic plant biomass via oxidation using lytic polysaccharide monooxygenases (LPMOs). The application of LPMOs is expected to contribute to ecologically friendly conversion of biomass into fuels and chemicals. Moreover, applications of LPMO-modified cellulose-based products may be envisaged within the food or material industry. RESULTS: Here, we show an up to 75-fold improvement in LPMO-driven cellulose degradation using polyphenol oxidase-activated lignin building blocks...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28434716/a-bioinformatics-analysis-of-3400-lytic-polysaccharide-oxidases-from-family-aa9
#4
Nicolas Lenfant, Matthieu Hainaut, Nicolas Terrapon, Elodie Drula, Vincent Lombard, Bernard Henrissat
Lytic polysaccharide monooxygenases of family AA9 catalyze the oxidative cleavage of glycosidic bonds in cellulose and related polysaccharides. The N-terminal half of AA9 LPMOs displays a huge sequence variability that is in contradiction with the substrate simplicity so far observed for these enzymes. To understand the cause of the high multigenicity that prevails in the family, we have performed a clustering analysis of the N-terminal region of 3400 sequences of family AA9 LPMOs, and have evaluated the coincidence of the clusters with distal visible features that may accompany functional differences...
April 13, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28417362/analyzing-activities-of-lytic-polysaccharide-monooxygenases-by-liquid-chromatography-and-mass-spectrometry
#5
Bjørge Westereng, Magnus Ø Arntzen, Jane Wittrup Agger, Gustav Vaaje-Kolstad, Vincent G H Eijsink
Lytic polysaccharide monooxygenases perform oxidative cleavage of glycosidic bonds in various polysaccharides. The majority of LMPOs studied so far possess activity on either cellulose or chitin and analysis of these activities is therefore the main focus of this review. Notably, however, the number of LPMOs that are active on other polysaccharides is increasing. The products generated by LPMOs from cellulose are either oxidized in the downstream end (at C1) or upstream end (at C4), or at both ends. These modifications only result in small structural changes, which makes both chromatographic separation and product identification by mass spectrometry challenging...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28411891/recombinant-expression-of-thermobifida-fusca-e7-lpmo-in-pichia-pastoris-and-escherichia-coli-and-their-functional-characterization
#6
Kelly B Rodrigues, Jéssica K A Macêdo, Tallyta Teixeira, Jéssica S Barros, Ana C B Araújo, Fernanda P Santos, Betânia F Quirino, Bruno S A F Brasil, Thaís F C Salum, Patrícia V Abdelnur, Léia C L Fávaro
The discovery of lytic polysaccharides monooxygenases copper dependent (LPMOs) revolutionized the classical concept that the cleavage of cellulose is a hydrolytic process in recent years. These enzymes carry out oxidative cleavage of cellulose (and other polysaccharides), acting synergistically with cellulases and other hydrolases. In fact, LPMOs have the potential for increasing the efficiency of the lignocellulosic biomass conversion in biofuels and high value chemicals. Among a small number of microbial LPMOs that have been characterized, some LPMOs were expressed and characterized biochemically from the bacteria Thermobifida fusca, using the host Escherichia coli...
April 9, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28394946/the-integrative-omics-of-white-rot-fungus-pycnoporus-coccineus-reveals-co-regulated-cazymes-for-orchestrated-lignocellulose-breakdown
#7
Shingo Miyauchi, David Navarro, Sacha Grisel, Didier Chevret, Jean-Guy Berrin, Marie-Noelle Rosso
Innovative green technologies are of importance for converting plant wastes into renewable sources for materials, chemicals and energy. However, recycling agricultural and forestry wastes is a challenge. A solution may be found in the forest. Saprotrophic white-rot fungi are able to convert dead plants into consumable carbon sources. Specialized fungal enzymes can be utilized for breaking down hard plant biopolymers. Thus, understanding the enzymatic machineries of such fungi gives us hints for the efficient decomposition of plant materials...
2017: PloS One
https://www.readbyqxmd.com/read/28366436/fast-purification-method-of-functional-lpmos-from-streptomyces-ambofaciens-by-affinity-adsorption
#8
Susana V Valenzuela, Guillem Ferreres, Gerard Margalef, F I Javier Pastor
A simple purification method by affinity adsorption was developed to obtain functional lytic polysaccharide monooxygenases (LPMOs). The system allows the successful purification to homogeneity of the most characterized bacterial LPMO, CBP21 from Serratia marcescens, and two LPMOs from Streptomyces ambofaciens, which have not been previously characterized. The first of these new LPMOs, named SamLPMO10B is a small enzyme (15 kDa) belonging to family 10 of auxiliary activities (AA10), showing activity on β-chitin...
February 21, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28364950/unliganded-and-substrate-bound-structures-of-the-cellooligosaccharide-active-lytic-polysaccharide-monooxygenase-lsaa9a-at-low-ph
#9
Kristian E H Frandsen, Jens-Christian N Poulsen, Tobias Tandrup, Leila Lo Leggio
Lytic polysaccharide monooxygenases (LPMOs) have been found to be key components in microbial (bacterial and fungal) degradation of biomass. They are copper metalloenzymes that degrade polysaccharides oxidatively and act in synergy with glycoside hydrolases. Recently crystallographic studies carried out at pH 5.5 of the LPMO from Lentinus similis belonging to the fungal LPMO family AA9 have provided the first atomic resolution view of substrate-LPMO interactions. The LsAA9A structure presented here determined at pH 3...
March 24, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28335986/on-the-formation-and-role-of-reactive-oxygen-species-in-light-driven-lpmo-oxidation-of-phosphoric-acid-swollen-cellulose
#10
K B Möllers, H Mikkelsen, T I Simonsen, D Cannella, K S Johansen, M J Bjerrum, C Felby
Light-driven activation of lytic polysaccharide monooxygenases (LPMOs) has been attributed to the transfer of high redox potential electrons from excited photopigments to the enzyme. However, due to the formation of reactive oxygen species (ROS) in such a system, not only electrons from the pigments but also ROS could be part of the enzyme mechanism. This work investigates the role of ROS in the oxidation of phosphoric acid swollen cellulose (PASC) by a light-driven LPMO system. Our results clearly show that the addition of superoxide dismutase or catalase to remove ROS did not attenuate the capacity of the light-driven LPMO system to oxidize PASC, as measured by formation of oxidized oligosaccharides...
March 18, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28302276/rp-uhplc-uv-esi-ms-ms-analysis-of-lpmo-generated-c4-oxidized-gluco-oligosaccharides-after-non-reductive-labeling-with-2-aminobenzamide
#11
Matthias Frommhagen, Gijs van Erven, Mark Sanders, Willem J H van Berkel, Mirjam A Kabel, Harry Gruppen
Lytic polysaccharide monooxygenases (LPMOs) are able to cleave recalcitrant polysaccharides, such as cellulose, by oxidizing the C1 and/or C4 atoms. The analysis of the resulting products requires a variety of analytical techniques. Up to now, these techniques mainly focused on the identification of non-oxidized and C1-oxidized oligosaccharides. The analysis of C4-oxidized gluco-oligosaccharides is mostly performed by using high pressure anion exchange chromatography (HPAEC). However, the alkaline conditions used during HPAEC analysis lead to tautomerization of C4-oxidized gluco-oligosaccharides, which limits the use of this technique...
March 6, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28293293/the-podospora-anserina-lytic-polysaccharide-monooxygenase-palpmo9h-catalyzes-oxidative-cleavage-of-diverse-plant-cell-wall-matrix-glycans
#12
Mathieu Fanuel, Sona Garajova, David Ropartz, Nicholas McGregor, Harry Brumer, Hélène Rogniaux, Jean-Guy Berrin
BACKGROUND: The enzymatic conversion of plant biomass has been recently revolutionized by the discovery of lytic polysaccharide monooxygenases (LPMO) that catalyze oxidative cleavage of polysaccharides. These powerful enzymes are secreted by a large number of fungal saprotrophs and are important components of commercial enzyme cocktails used for industrial biomass conversion. Among the 33 AA9 LPMOs encoded by the genome of Podospora anserina, the PaLPMO9H enzyme catalyzes mixed C1/C4 oxidative cleavage of cellulose and cello-oligosaccharides...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28291519/structural-studies-of-neurospora-crassa-lpmo9d-and-redox-partner-cdhiia-using-neutron-crystallography-and-small-angle-scattering
#13
Annette M Bodenheimer, William B O'Dell, Christopher B Stanley, Flora Meilleur
Sensitivity to hydrogen/deuterium and lack of observable radiation damage makes cold neutrons an ideal probe the structural studies of proteins with highly photosensitive groups such as the copper center of lytic polysaccharide monooxygenases (LPMOs) and flavin adenine dinucleotide (FAD) and heme redox cofactors of cellobiose dehydrogenases (CDHs). Here, neutron crystallography and small-angle neutron scattering are used to investigate Neurospora crassa LPMO9D (NcLPMO9D) and CDHIIA (NcCDHIIA), respectively...
March 4, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28291518/a-novel-expression-system-for-lytic-polysaccharide-monooxygenases
#14
Gaston Courtade, Simone Balzer Le, Gerd Inger Sætrom, Trygve Brautaset, Finn L Aachmann
Lytic polysaccharide monooxygenases (LPMOs) are key enzymatic players of lignocellulosic biomass degradation processes. As such, they have been introduced in cellulolytic cocktails for more efficient and less expensive lignocellulose saccharification. The recombinant production of LPMOs in bacteria for scientific investigations using vectors typically based on the T7 and lacUV5 promoters has been hampered by low yields. Reasons for this have been catabolite repression when producing the proteins in defined media with glucose as the sole carbon source, as well as the lack of an inducible expression system that allows controlled production of LPMOs that are correctly processed during translocation to the periplasmic space...
February 14, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28250814/type-dependent-action-modes-of-ttaa9e-and-taaa9a-acting-on-cellulose-and-differently-pretreated-lignocellulosic-substrates
#15
In Jung Kim, Nari Seo, Hyun Joo An, Jae-Han Kim, Paul V Harris, Kyoung Heon Kim
BACKGROUND: Lytic polysaccharide monooxygenase (LPMO) is a group of recently identified proteins that catalyze oxidative cleavage of the glycosidic linkages of cellulose and other polysaccharides. By utilizing the oxidative mode of action, LPMOs are able to enhance the efficiency of cellulase in the hydrolysis of cellulose. Particularly, auxiliary activity family 9 (AA9) is a group of fungal LPMOs that show a type-dependent regioselectivity on cellulose in which Types 1, 2, and 3 hydroxylate at C1, C4, and C1 and C4 positions, respectively...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28222763/the-discovery-of-novel-lpmo-families-with-a-new-hidden-markov-model
#16
Gerben P Voshol, Erik Vijgenboom, Peter J Punt
BACKGROUND: Renewable biopolymers, such as cellulose, starch and chitin are highly resistance to enzymatic degradation. Therefore, there is a need to upgrade current degradation processes by including novel enzymes. Lytic polysaccharide mono-oxygenases (LPMOs) can disrupt recalcitrant biopolymers, thereby enhancing hydrolysis by conventional enzymes. However, novel LPMO families are difficult to identify using existing methods. Therefore, we developed a novel profile Hidden Markov model (HMM) and used it to mine genomes of ascomycetous fungi for novel LPMOs...
February 21, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28177316/crystallization-of-a-fungal-lytic-polysaccharide-monooxygenase-expressed-from-glycoengineered-pichia-pastoris-for-x-ray-and-neutron-diffraction
#17
William B O'Dell, Paul D Swartz, Kevin L Weiss, Flora Meilleur
Lytic polysaccharide monooxygenases (LPMOs) are carbohydrate-disrupting enzymes secreted by bacteria and fungi that break glycosidic bonds via an oxidative mechanism. Fungal LPMOs typically act on cellulose and can enhance the efficiency of cellulose-hydrolyzing enzymes that release soluble sugars for bioethanol production or other industrial uses. The enzyme PMO-2 from Neurospora crassa (NcPMO-2) was heterologously expressed in Pichia pastoris to facilitate crystallographic studies of the fungal LPMO mechanism...
February 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28140411/charge-transport-mechanisms-in-sol-gel-grown-la0-7pb0-3mno3-laalo3-manganite-films
#18
Eesh Vaghela, M J Keshvani, Keval Gadani, Zalak Joshi, Hetal Boricha, K Asokan, D Venkateshwarlu, V Ganesan, N A Shah, P S Solanki
In this communication, structural, microstructural, transport and magnetotransport properties are reported for La0.7Pb0.3MnO3/LaAlO3 (LPMO/LAO) manganite films having different thicknesses. All the films were irradiated with 200 MeV Ag(+15) swift heavy ions (SHI). Films were grown using the sol-gel method by employing the acetate precursor route. Structural measurements were carried out using the X-ray diffraction (XRD) method at room temperature, while atomic force microscopy (AFM) was performed for the surface morphology...
January 31, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28125213/microplate-based-detection-of-lytic-polysaccharide-monooxygenase-activity-by-fluorescence-labeling-of-insoluble-oxidized-products
#19
Thu V Vuong, Bing Liu, Mats Sandgren, Emma R Master
Most existing methods for screening the activity of lytic polysaccharide mono-oxygenases (LPMOs) on polysaccharides are based on the detection of soluble oxidized sugars. This approach might underestimate the total performance of LPMOs since oxidation events that do not lead to oligosaccharide release are not detected. Using PcLPMO9D as a model enzyme, a microplate-based method has been developed to detect C1-oxidizing LPMO activity by covalently linking a water-soluble fluorophore to oxidized positions within the cellulose fiber...
January 26, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28123349/metadata-analysis-of-phanerochaete-chrysosporium-gene-expression-data-identified-common-cazymes-encoding-gene-expression-profiles-involved-in-cellulose-and-hemicellulose-degradation
#20
Ayyappa Kumar Sista Kameshwar, Wensheng Qin
In literature, extensive studies have been conducted on popular wood degrading white rot fungus, Phanerochaete chrysosporium about its lignin degrading mechanisms compared to the cellulose and hemicellulose degrading abilities. This study delineates cellulose and hemicellulose degrading mechanisms through large scale metadata analysis of P. chrysosporium gene expression data (retrieved from NCBI GEO) to understand the common expression patterns of differentially expressed genes when cultured on different growth substrates...
2017: International Journal of Biological Sciences
keyword
keyword
74392
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"